Rh5BG162600

Class I heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016287
Physical Location & Seq
Forward (+)
16955513 .. 16956676
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG162600.1

Sequence Viewer

Length: 663 bp
ATGGACCCAAAAAAAAAATCGATCAAACTCTATCCAAATCTAGAAATTTCAAGAAATCACTGTCCCCTCTTGCCCTCCAATATAAATATACCCATTCACCAGCAAACTGATCCAGACCAAAACAATCCAATTAAACAACACCTAAGAAGAAGCTCAGTTTCTCAAACCCTATTAACAGAGTCCCAGAAAGGCAGAAAATTAACAACAATGTCCCTCTTGCAATCTCTCTTGGATCCTTTCAGAGTTTTCAACTCAGATTTGGACGATACTCAAATGGACTGGAAGGAGACCTCGAACTCCCACGTTTTCGAAATTGATCTTCCTGGCCTTACAAAAGAGGACGTGAAGCTTGAACTGCATGAAGACAGAGTGCTTCATGTTAGTGCAGAGAGAAAGGATGAAGAGCCTCCCGAAGGGGATCAGAAGATTAAGGAGACGTGGCACTGCAGGGAGAGATCAAGTGGCAGCTTCTCGAAGCATTTCCGGTTGCCTGAGAATGCCAAGGTTGATGAGATTAAGGCTTCAATGCGTGATGGGGTGTTAGTCATTACGGTGCCAAAGGAGGACTTGAAGAAAAAGCCTAAACACAAAGAAGTTGAAATCTCGGGGGATGAAGAGCACGTTTCTAAAGGACTTGGTCGTTTTGTGTGCTGCAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

220

Amino Acids

25.29

Weight (kDa)

6.8

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 93 - 202 3.3e-26 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016287)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13900
malus_domestica MD05G1240300.v1.1 MD10G1218300.v1.1
prunus_persica Prupe.4G125800_v2.0.a1
pyrus_communis pycom05g21630
rosa_chinensis RchiOBHm_Chr5g0023111
rosa_laevigata RLG00000032699
rosa_roxburghii Rroxscaffold_1G00056240
rosa_rugosa Rorug05G0077200
rosa_samantha Rh5AG163300 Rh5BG162600 Rh5CG177500 Rh5DG163900
rosa_wichuraiana Rw5G014730 Rw5G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 553
AclWI GGATC 4 cut(s) 104, 227, 240, 426
AcsI RAATTY 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 6 cut(s) 51, 250, 353, 525, 571, 599
AjiI CACGTC 2 cut(s) 343, 438
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 4 cut(s) 153, 349, 468, 659
AluI AGCT 4 cut(s) 153, 349, 468, 659
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 2 cut(s) 281, 428
AlwI GGATC 4 cut(s) 104, 227, 240, 426
Ama87I CYCGRG 1 cut(s) 604
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 2 cut(s) 465, 651
ApoI RAATTY 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 479
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 89
AsuII TTCGAA 1 cut(s) 309
AvaI CYCGRG 1 cut(s) 604
AvaII GGWCC 1 cut(s) 4
BamHI GGATCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 553
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 2 cut(s) 477, 638
BccI CCATC 1 cut(s) 527
BciT130I CCWGG 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 281, 428
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 445
BisI GCNGC 2 cut(s) 466, 652
BlsI GCNGC 2 cut(s) 467, 653
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 604
BmgBI CACGTC 2 cut(s) 343, 438
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 3 cut(s) 6, 234, 555
BmrFI CCNGG 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 501
BsaWI WCCGGW 1 cut(s) 483
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 324
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 2 cut(s) 403, 616
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 3 cut(s) 168, 267, 483
BseNI ACTGG 1 cut(s) 284
BseXI GCAGC 2 cut(s) 477, 638
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 1 cut(s) 327
BshNI GGYRCC 1 cut(s) 553
BshVI ATCGAT 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 604
BsiSI CCGG 1 cut(s) 484
BslFI GGGAC 3 cut(s) 48, 166, 196
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 2 cut(s) 281, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 3 cut(s) 48, 166, 196
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 281
BsoBI CYCGRG 1 cut(s) 604
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 6 cut(s) 21, 109, 232, 316, 418, 455
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 3 cut(s) 167, 266, 484
BspDI ATCGAT 1 cut(s) 20
BspLI GGNNCC 3 cut(s) 6, 234, 555
BspMAI CTGCAG 1 cut(s) 449
BspPI GGATC 4 cut(s) 104, 227, 240, 426
BspQI GCTCTTC 2 cut(s) 396, 609
BspT104I TTCGAA 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 553
BspTNI GGTCTC 1 cut(s) 281
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 6 cut(s) 21, 109, 232, 316, 418, 455
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 62, 553
Bst6I CTCTTC 2 cut(s) 396, 609
BstBI TTCGAA 1 cut(s) 309
BstDEI CTNAG 4 cut(s) 143, 154, 253, 492
BstENI CCTNNNNNAGG 1 cut(s) 411
BstF5I GGATG 2 cut(s) 403, 616
BstKTI GATC 6 cut(s) 24, 112, 235, 319, 421, 458
BstMAI GTCTC 2 cut(s) 281, 428
BstMBI GATC 6 cut(s) 21, 109, 232, 316, 418, 455
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 445
BstV1I GCAGC 2 cut(s) 477, 638
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 20
BsuRI GGCC 1 cut(s) 327
BsuTUI ATCGAT 1 cut(s) 20
BtrI CACGTC 2 cut(s) 343, 438
BtsCI GGATG 2 cut(s) 403, 616
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 58, 442
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 20
CviAII CATG 2 cut(s) 359, 377
CviJI RGCY 8 cut(s) 153, 327, 349, 406, 468, 521, 580, 659
CviKI_1 RGCY 8 cut(s) 153, 327, 349, 406, 468, 521, 580, 659
DdeI CTNAG 4 cut(s) 143, 154, 253, 492
DpnI GATC 6 cut(s) 23, 111, 234, 318, 420, 457
DpnII GATC 6 cut(s) 21, 109, 232, 316, 418, 455
Eam1104I CTCTTC 2 cut(s) 396, 609
EarI CTCTTC 2 cut(s) 396, 609
Eco130I CCWWGG 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 1 cut(s) 604
EcoNI CCTNNNNNAGG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 2 cut(s) 362, 380
FaiI YATR 4 cut(s) 83, 89, 360, 378
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 3 cut(s) 48, 166, 196
FatI CATG 2 cut(s) 358, 376
Fnu4HI GCNGC 2 cut(s) 466, 652
FokI GGATG 2 cut(s) 410, 623
Fsp4HI GCNGC 2 cut(s) 466, 652
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 466, 652
HaeIII GGCC 1 cut(s) 327
HapII CCGG 1 cut(s) 484
Hin1II CATG 2 cut(s) 362, 380
HindIII AAGCTT 2 cut(s) 347, 657
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 484
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 3 cut(s) 242, 256, 423
Hpy188III TCNNGA 5 cut(s) 41, 51, 113, 410, 472
HpyAV CCTTC 2 cut(s) 277, 407
HpyCH4III ACNGT 2 cut(s) 62, 553
HpyCH4IV ACGT 4 cut(s) 303, 342, 437, 621
HpyCH4V TGCA 5 cut(s) 220, 358, 386, 447, 654
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 4 cut(s) 143, 154, 253, 492
HpySE526I ACGT 4 cut(s) 303, 342, 437, 621
Hsp92II CATG 2 cut(s) 362, 380
Kzo9I GATC 6 cut(s) 21, 109, 232, 316, 418, 455
LguI GCTCTTC 2 cut(s) 396, 609
LpnPI CCDG 9 cut(s) 113, 126, 197, 265, 309, 336, 433, 497, 504
Lsp1109I GCAGC 2 cut(s) 477, 638
MaeI CTAG 1 cut(s) 41
MaeII ACGT 4 cut(s) 303, 342, 437, 621
MalI GATC 6 cut(s) 23, 111, 234, 318, 420, 457
MboI GATC 6 cut(s) 21, 109, 232, 316, 418, 455
MboII GAAGA 7 cut(s) 159, 311, 374, 413, 436, 583, 626
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 4 cut(s) 45, 129, 197, 312
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 7 cut(s) 77, 85, 224, 301, 331, 417, 556
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 6 cut(s) 132, 173, 200, 429, 516, 661
MslI CAYNNNNRTG 2 cut(s) 381, 551
MspI CCGG 1 cut(s) 484
MspR9I CCNGG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 502
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 355
NdeII GATC 6 cut(s) 21, 109, 232, 316, 418, 455
NlaIII CATG 2 cut(s) 362, 380
NlaIV GGNNCC 3 cut(s) 6, 234, 555
NspV TTCGAA 1 cut(s) 309
PciSI GCTCTTC 2 cut(s) 396, 609
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 479
PflFI GACNNNGTC 1 cut(s) 636
PkrI GCNGC 2 cut(s) 467, 653
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 3 cut(s) 6, 234, 555
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 232
PsyI GACNNNGTC 1 cut(s) 636
RseI CAYNNNNRTG 2 cut(s) 381, 551
SapI GCTCTTC 2 cut(s) 396, 609
SaqAI TTAA 6 cut(s) 132, 173, 200, 429, 516, 661
SatI GCNGC 2 cut(s) 466, 652
Sau3AI GATC 6 cut(s) 21, 109, 232, 316, 418, 455
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 1 cut(s) 621
SfcI CTRYAG 1 cut(s) 445
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 381, 551
Sse9I AATT 4 cut(s) 45, 129, 197, 312
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 62, 553
TaiI ACGT 4 cut(s) 306, 345, 440, 624
TaqI TCGA 4 cut(s) 20, 293, 309, 473
TasI AATT 4 cut(s) 45, 129, 197, 312
Tru1I TTAA 6 cut(s) 132, 173, 200, 429, 516, 661
Tru9I TTAA 6 cut(s) 132, 173, 200, 429, 516, 661
TscAI CASTG 2 cut(s) 65, 449
TseI GCWGC 2 cut(s) 465, 651
TspDTI ATGAA 4 cut(s) 365, 375, 414, 627
TspRI CASTG 2 cut(s) 65, 449
Tth111I GACNNNGTC 1 cut(s) 636
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 411
XapI RAATTY 1 cut(s) 45
XbaI TCTAGA 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.