MD05G1245500.v1.1
ERF Family

Protein kinase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014690
Physical Location & Seq
Reverse (-)
37741241 .. 37743532
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1245500.v1.1.491

Sequence Viewer

Length: 2292 bp
ATGTTAGCAATGAAATTAGGTTACCAACATAACGATTTCGATTCGTCTTTGGAGCAAAGTTTGAGTGGAAGTTTCAGAAAGTTCACATCTGGTTTGCTGCAAAATGATATGGGACTGCTGTCTCCGGGCCATAGCTCGAATGGTAGTTTTCGGAGGTCTAACTCCGTTATTTCCACACAGTCCGCCGCTTCAAGTAAGTATGCCTCTTCTTCCAGAAGAGTGTCCAATGGGCTAAAGGACTATGCAAGGAAACTTGCTGACCTCGAGTTGTTCACACATTGTATTGAGGATTGGGTTTCGGAGAATACGTGTGAGGATTCAGACACTGGTTTCAGTCCCCCATTCATGATTGATGAATTGCGCAAGCTTGATGTGGCGTTGGAGGGTGTTCTGTTTCAGCAACTCGTCCGGATGCCATGCTCACCTAATGTTTCCGGTGATCCAAATGAAGATGAGTATCTTGCGTTGGAAGACTTCCTTCAGGCTGTAGTGAGTGGTTTATGGCATGCATTTTGGCATAAAAGAGGTGAGCTGCCGCTATTTGTATCTTGTCCGCGTTCTCTTGGATCCAAGTTTTATAGTGTAGAGAAGGCAATATCAAGGGGAGGGCTTAGAAAGCTCTGCGGTTTATCTTTGATATCGAAAATTGGGGGTGATCAGAAAGTCCAATGGGATCAGATCATGGAGTTTGCCCTGTTTAAAGCTGATATATTGTCTGGAAATGAGATGAAATTATCAGCTCCCGTTATTTGTGAAGCCCTCTTTTATGGTTTTCATATACTTGTTTCTAGGTGTTTGAGCAAGGTTAAAGCTACAAAGAGCAGTTCTGTTTTCCTTTTGGTTCTGGATTCTAATTATGGAGGGGTGGTTAAACTTGGCGGTGATCTTAGTAAACTCGATTTAAGCTCAAGTAATCCGTACAAGTCTGCAGTTGAATGGATCAAAAATCATGCTGAAGTTTGTGTTTCTCCAGTGGACCGGATATGGAACAAATTCGGGAATGCAAATTGGGGGGACCTAGGAACTCTGCAGGTACTTTTGGCAACTTACAATGCTATTGTACAATGGAATGGACTGCCTAGGAAGTCAGTAGCTTCATTAGTCTCAGATCATAGCCTTCGCCTTCAGAAGCGTAGAATGGAGTTCTGCCTCAGTCAGAATGGGAATGTTCTGGTTCCCTTCCAACAATCTAGCCACCAGCAAGAAGATATTGTTGAAGTTGACCAAACTAATGGTCGAGCTGTCAAACACAATGCTTCGCGCTTAAGGCTTAATCAGGGCGAAGTTTTGCTACTGGAAGATCAACAGCAGGGGCAGAAAACTTTCCAAGTACAAGAGTCTCTGGTGGGAGGGAACCATTACCTCTATAGTGCGGTTTGTGTAGATTGTCCGGCGCAGCTGTTGACTTTATACATTGGCGCGCATCCGACCAGACTTGAACCTTGTTGGGAGGACATGAGTCTTTGGTACCAAGTCCAGAGGCAAACAAAAGTGCTAAACATCTTCAAGCATCAGGGAATTACAAGCAAGTATTTGCCGGAAATGATTGCCTCAGGGCGGATTTTGCATTCTGGTCCCTGCAAGAAGCAGACCCCCGGGGGTCGATGTGATCATCCGTTGTGTGGGACTCCAATACTAGTGACATCTCCAGTTGGTGAACCTGTTTCATACGTTGTTTCTCAAGATGGCCCCCTTTCCCCTGAGGAGGCAATTCGCTGCTGCAGAGACTGCCTAGCTGCTCTAAGAAGTGCAGCAATGCCAAATGTGCAACATGGTGATATTTGTCCGGAAAACATAATTCGGGTTGTTGACGAACAAGGCTCAAGAAACAGCAGTTTCTATGTTCCAATATCATGGGGCCGCGCAGTTTTGGAAGACCGGGACAGTCCAGCCATAAACCTACAATTCTCTTCATCACATGCACTTCAGCATGGGAAACTTTGTCCATCATCAGACGCGGAAAGCCTTGTTTACCTCATGTTGTTCGTATGCGGTGGAGCAATGCAGCAGCAGGACTCCATCGAATCTGCGTTGCAATGGAGGGAGACGAGCTGGGCAAAGCGCTCAATCCAGCAGCAGCTAGGTGAGGTTTCACCTCTCCTCAAGGCATTTGCTGACTATGTGGACAGCCTTTGTGGAACACCATATCCTGTGGACTATGACATATGGTTGAACAGATTGAGCAGGGCCGTGGATGGCGTGGGTGAGAGGGGTAAAATGATCGAAGAAGTGGCGGTTGCGTTGAGGTTGAAGGATGTTGCTGAGTCCTCGGGGACATCTGCAGGGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

764

Amino Acids

84.3

Weight (kDa)

6.17

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13040
malus_domestica MD05G1245500.v1.1 MD10G1226800.v1.1
prunus_persica Prupe.4G118400_v2.0.a1
pyrus_communis pycom05g22160 pycom10g19070
rosa_chinensis RchiOBHm_Chr5g0021231
rosa_laevigata RLG00000032597
rosa_multiflora Rmu_sc0003392.1_g000017 Rmu_sc0004847.1_g000001
rosa_roxburghii Rroxscaffold_1G00057270
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154400 Rh5BG154000 Rh5CG167600 Rh5DG154500
rosa_wichuraiana Rw5G013760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 362
Acc36I ACCTGC 1 cut(s) 1021
Acc65I GGTACC 1 cut(s) 1467
AccB1I GGYRCC 1 cut(s) 1467
AccII CGCG 5 cut(s) 556, 1261, 1421, 1863, 1958
AccIII TCCGGA 2 cut(s) 408, 1786
AclWI GGATC 5 cut(s) 434, 561, 574, 681, 947
AcsI RAATTY 1 cut(s) 992
AcuI CTGAAG 4 cut(s) 464, 975, 1109, 1910
AfaI GTAC 5 cut(s) 920, 1035, 1062, 1332, 1469
AfeI AGCGCT 1 cut(s) 2063
AfiI CCNNNNNNNGG 3 cut(s) 1557, 1622, 1705
AflII CTTAAG 1 cut(s) 1264
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 7 cut(s) 192, 935, 1217, 1439, 1507, 2173, 2251
AhlI ACTAGT 1 cut(s) 1636
AloI GAACNNNNNNTCC 2 cut(s) 2131, 2163
Alw26I GTCTC 5 cut(s) 126, 1108, 1344, 1719, 2039
AlwI GGATC 5 cut(s) 434, 561, 574, 681, 947
AlwNI CAGNNNCTG 2 cut(s) 326, 1728
Ama87I CYCGRG 3 cut(s) 263, 1595, 2269
Aor13HI TCCGGA 2 cut(s) 408, 1786
Aor51HI AGCGCT 1 cut(s) 2063
AoxI GGCC 4 cut(s) 127, 1687, 1858, 2187
ApoI RAATTY 1 cut(s) 992
ArsI GACNNNNNNTTYG 2 cut(s) 1219, 1251
Asp700I GAANNNNTTC 2 cut(s) 992, 1322
Asp718I GGTACC 1 cut(s) 1467
AspA2I CCTAGG 2 cut(s) 1018, 1079
AspLEI GCGC 7 cut(s) 363, 1263, 1396, 1421, 1423, 1865, 2064
AspS9I GGNCC 8 cut(s) 127, 976, 1015, 1574, 1688, 1858, 2187, 2286
AsuC2I CCSGG 4 cut(s) 126, 1596, 1597, 1880
AvaI CYCGRG 3 cut(s) 263, 1595, 2269
AvaII GGWCC 4 cut(s) 976, 1015, 1574, 2286
AvrII CCTAGG 2 cut(s) 1018, 1079
AxyI CCTNAGG 2 cut(s) 1552, 1701
BamHI GGATCC 1 cut(s) 566
BanI GGYRCC 1 cut(s) 1467
BarI GAAGNNNNNNTAC 2 cut(s) 1275, 1307
BbsI GAAGAC 2 cut(s) 477, 1881
BccI CCATC 4 cut(s) 1679, 1954, 2027, 2189
BceAI ACGGC 1 cut(s) 2174
BclI TGATCA 2 cut(s) 655, 1609
BcnI CCSGG 4 cut(s) 126, 1596, 1597, 1880
BcoDI GTCTC 5 cut(s) 126, 1108, 1344, 1719, 2039
BcuI ACTAGT 1 cut(s) 1636
BfaI CTAG 8 cut(s) 789, 1019, 1080, 1191, 1637, 1733, 2081, 2290
BfmI CTRYAG 6 cut(s) 486, 927, 1028, 1366, 1720, 2280
BfoI RGCGCY 1 cut(s) 2065
BfrI CTTAAG 1 cut(s) 1264
BfuAI ACCTGC 1 cut(s) 1021
BlnI CCTAGG 2 cut(s) 1018, 1079
Bme1390I CCNGG 4 cut(s) 126, 1596, 1597, 1880
Bme18I GGWCC 4 cut(s) 976, 1015, 1574, 2286
BmeT110I CYCGRG 3 cut(s) 263, 1595, 2269
BmgT120I GGNCC 8 cut(s) 127, 976, 1015, 1574, 1688, 1858, 2187, 2286
BmiI GGNNCC 9 cut(s) 568, 1016, 1176, 1355, 1469, 1576, 1690, 1859, 2287
BmrFI CCNGG 4 cut(s) 126, 1596, 1597, 1880
BmsI GCATC 3 cut(s) 402, 1432, 1519
BoxI GACNNNNGTC 2 cut(s) 118, 1458
BpiI GAAGAC 2 cut(s) 477, 1881
BpmI CTGGAG 2 cut(s) 954, 1632
BpuEI CTTGAG 4 cut(s) 892, 1665, 1807, 2087
BpuMI CCSGG 4 cut(s) 126, 1596, 1597, 1880
BsaAI YACGTR 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 7 cut(s) 1018, 1079, 1594, 1595, 1596, 2190, 2268
BsaWI WCCGGW 4 cut(s) 408, 434, 978, 1786
BsaXI ACNNNNNCTCC 4 cut(s) 293, 323, 852, 882
Bsc4I CCNNNNNNNGG 3 cut(s) 1557, 1622, 1705
Bse1I ACTGG 4 cut(s) 331, 971, 1299, 1649
Bse21I CCTNAGG 2 cut(s) 1552, 1701
Bse3DI GCAATG 4 cut(s) 15, 1761, 2007, 2042
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 2 cut(s) 408, 1786
BseDI CCNNGG 7 cut(s) 1018, 1079, 1594, 1595, 1596, 2190, 2268
BseGI GGATG 5 cut(s) 417, 1423, 1612, 2200, 2260
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 1557, 1622, 1705
BseMI GCAATG 4 cut(s) 15, 1761, 2007, 2042
BseMII CTCAG 5 cut(s) 1119, 1165, 1566, 1692, 2253
BseNI ACTGG 4 cut(s) 331, 971, 1299, 1649
BsePI GCGCGC 1 cut(s) 1419
BseRI GAGGAG 2 cut(s) 1718, 2090
BseYI CCCAGC 1 cut(s) 2052
BsgI GTGCAG 1 cut(s) 1770
Bsh1236I CGCG 5 cut(s) 556, 1261, 1421, 1863, 1958
BshFI GGCC 4 cut(s) 129, 1689, 1860, 2189
BshNI GGYRCC 1 cut(s) 1467
BsiHKCI CYCGRG 3 cut(s) 263, 1595, 2269
BsiSI CCGG 9 cut(s) 125, 409, 435, 979, 1391, 1538, 1596, 1787, 1879
BslFI GGGAC 7 cut(s) 126, 321, 1028, 1560, 1639, 1895, 2287
BslI CCNNNNNNNGG 3 cut(s) 1557, 1622, 1705
BsmAI GTCTC 5 cut(s) 126, 1108, 1344, 1719, 2039
BsmBI CGTCTC 1 cut(s) 2039
BsmFI GGGAC 7 cut(s) 126, 321, 1028, 1560, 1639, 1895, 2287
BsmI GAATGC 2 cut(s) 1006, 1567
BsnI GGCC 4 cut(s) 129, 1689, 1860, 2189
BsoBI CYCGRG 3 cut(s) 263, 1595, 2269
Bsp13I TCCGGA 2 cut(s) 408, 1786
Bsp1407I TGTACA 1 cut(s) 1060
BspANI GGCC 4 cut(s) 129, 1689, 1860, 2189
BspCNI CTCAG 5 cut(s) 1118, 1164, 1565, 1693, 2254
BspEI TCCGGA 2 cut(s) 408, 1786
BspFNI CGCG 5 cut(s) 556, 1261, 1421, 1863, 1958
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 9 cut(s) 568, 1016, 1176, 1355, 1469, 1576, 1690, 1859, 2287
BspMAI CTGCAG 4 cut(s) 931, 1032, 1724, 2284
BspMI ACCTGC 1 cut(s) 1021
BspPI GGATC 5 cut(s) 434, 561, 574, 681, 947
BspT107I GGYRCC 1 cut(s) 1467
BspTI CTTAAG 1 cut(s) 1264
BsrDI GCAATG 4 cut(s) 15, 1761, 2007, 2042
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 4 cut(s) 331, 971, 1299, 1649
BssECI CCNNGG 7 cut(s) 1018, 1079, 1594, 1595, 1596, 2190, 2268
BssHII GCGCGC 1 cut(s) 1419
BssT1I CCWWGG 2 cut(s) 1018, 1079
Bst4CI ACNGT 2 cut(s) 180, 1886
Bst6I CTCTTC 3 cut(s) 211, 211, 1915
BstAFI CTTAAG 1 cut(s) 1264
BstAPI GCANNNNNTGC 1 cut(s) 1728
BstAUI TGTACA 1 cut(s) 1060
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 3 cut(s) 365, 507, 1421
BstDEI CTNAG 8 cut(s) 611, 887, 1105, 1151, 1552, 1701, 1742, 2262
BstDSI CCRYGG 1 cut(s) 2190
BstEII GGTNACC 1 cut(s) 20
BstF5I GGATG 5 cut(s) 417, 1423, 1612, 2200, 2260
BstFNI CGCG 5 cut(s) 556, 1261, 1421, 1863, 1958
BstH2I RGCGCY 1 cut(s) 2065
BstHHI GCGC 7 cut(s) 363, 1263, 1396, 1421, 1423, 1865, 2064
BstMAI GTCTC 5 cut(s) 126, 1108, 1344, 1719, 2039
BstMWI GCNNNNNNNGC 5 cut(s) 616, 1267, 1564, 1728, 1765
BstNSI RCATGY 2 cut(s) 509, 1922
BstPAI GACNNNNGTC 2 cut(s) 118, 1458
BstPI GGTNACC 1 cut(s) 20
BstSCI CCNGG 4 cut(s) 124, 1594, 1595, 1878
BstSFI CTRYAG 6 cut(s) 486, 927, 1028, 1366, 1720, 2280
BstUI CGCG 5 cut(s) 556, 1261, 1421, 1863, 1958
BstV2I GAAGAC 2 cut(s) 477, 1881
BstX2I RGATCY 1 cut(s) 566
BstXI CCANNNNNNTGG 2 cut(s) 1232, 1854
BstYI RGATCY 1 cut(s) 566
Bsu36I CCTNAGG 2 cut(s) 1552, 1701
BsuRI GGCC 4 cut(s) 129, 1689, 1860, 2189
BtgI CCRYGG 1 cut(s) 2190
BtsCI GGATG 5 cut(s) 417, 1423, 1612, 2200, 2260
BtsIMutI CAGTG 2 cut(s) 324, 978
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 3 cut(s) 365, 507, 1421
CaiI CAGNNNCTG 2 cut(s) 326, 1728
CciI TCATGA 1 cut(s) 345
CfoI GCGC 7 cut(s) 363, 1263, 1396, 1421, 1423, 1865, 2064
Cfr13I GGNCC 8 cut(s) 127, 976, 1015, 1574, 1688, 1858, 2187, 2286
Cfr9I CCCGGG 1 cut(s) 1595
CseI GACGC 1 cut(s) 1964
Csp6I GTAC 5 cut(s) 919, 1034, 1061, 1331, 1468
CviQI GTAC 5 cut(s) 919, 1034, 1061, 1331, 1468
DdeI CTNAG 8 cut(s) 611, 887, 1105, 1151, 1552, 1701, 1742, 2262
DraI TTTAAA 1 cut(s) 700
Eam1104I CTCTTC 3 cut(s) 211, 211, 1915
EarI CTCTTC 3 cut(s) 211, 211, 1915
EciI GGCGGA 2 cut(s) 172, 1573
Eco130I CCWWGG 2 cut(s) 1018, 1079
Eco32I GATATC 1 cut(s) 639
Eco47I GGWCC 4 cut(s) 976, 1015, 1574, 2286
Eco47III AGCGCT 1 cut(s) 2063
Eco57I CTGAAG 4 cut(s) 464, 975, 1109, 1910
Eco81I CCTNAGG 2 cut(s) 1552, 1701
Eco88I CYCGRG 3 cut(s) 263, 1595, 2269
Eco91I GGTNACC 1 cut(s) 20
EcoO109I RGGNCCY 2 cut(s) 1015, 2286
EcoO65I GGTNACC 1 cut(s) 20
EcoRV GATATC 1 cut(s) 639
EcoT14I CCWWGG 2 cut(s) 1018, 1079
EcoT22I ATGCAT 1 cut(s) 511
ErhI CCWWGG 2 cut(s) 1018, 1079
Esp3I CGTCTC 1 cut(s) 2039
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 7 cut(s) 126, 321, 1028, 1560, 1639, 1895, 2287
FauNDI CATATG 1 cut(s) 2165
FbaI TGATCA 2 cut(s) 655, 1609
FokI GGATG 5 cut(s) 424, 1410, 1599, 2207, 2267
FspBI CTAG 8 cut(s) 789, 1019, 1080, 1191, 1637, 1733, 2081, 2290
FspI TGCGCA 1 cut(s) 362
GlaI GCGC 7 cut(s) 362, 1262, 1395, 1420, 1422, 1864, 2063
GsaI CCCAGC 1 cut(s) 2056
GsuI CTGGAG 2 cut(s) 954, 1632
HaeII RGCGCY 1 cut(s) 2065
HaeIII GGCC 4 cut(s) 129, 1689, 1860, 2189
HapII CCGG 9 cut(s) 125, 409, 435, 979, 1391, 1538, 1596, 1787, 1879
HgaI GACGC 1 cut(s) 1964
HhaI GCGC 7 cut(s) 363, 1263, 1396, 1421, 1423, 1865, 2064
Hin6I GCGC 7 cut(s) 361, 1261, 1394, 1419, 1421, 1863, 2062
HinP1I GCGC 7 cut(s) 361, 1261, 1394, 1419, 1421, 1863, 2062
HincII GTYRAC 3 cut(s) 1222, 1404, 1810
HindII GTYRAC 3 cut(s) 1222, 1404, 1810
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 9 cut(s) 41, 317, 848, 1337, 1459, 1627, 2015, 2024, 2264
HpaII CCGG 9 cut(s) 125, 409, 435, 979, 1391, 1538, 1596, 1787, 1879
HpyAV CCTTC 6 cut(s) 488, 583, 1127, 1133, 1189, 2245
HpyCH4III ACNGT 2 cut(s) 180, 1886
HpyCH4IV ACGT 2 cut(s) 308, 1671
HpyF10VI GCNNNNNNNGC 5 cut(s) 616, 1267, 1564, 1728, 1765
HpyF3I CTNAG 8 cut(s) 611, 887, 1105, 1151, 1552, 1701, 1742, 2262
HpySE526I ACGT 2 cut(s) 308, 1671
HspAI GCGC 7 cut(s) 361, 1261, 1394, 1419, 1421, 1863, 2062
Kpn2I TCCGGA 2 cut(s) 408, 1786
KpnI GGTACC 1 cut(s) 1471
Ksp22I TGATCA 2 cut(s) 655, 1609
LmnI GCTCC 3 cut(s) 52, 745, 1997
LweI GCATC 3 cut(s) 402, 1432, 1519
MaeI CTAG 8 cut(s) 789, 1019, 1080, 1191, 1637, 1733, 2081, 2290
MaeII ACGT 2 cut(s) 308, 1671
MaeIII GTNAC 2 cut(s) 20, 1639
MflI RGATCY 1 cut(s) 566
MlyI GAGTC 5 cut(s) 1346, 1468, 1621, 2009, 2273
MmeI TCCRAC 4 cut(s) 360, 447, 1207, 1451
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 2 cut(s) 408, 1786
MroXI GAANNNNTTC 2 cut(s) 992, 1322
MseI TTAA 6 cut(s) 699, 807, 870, 902, 1265, 1272
MspA1I CMGCKG 1 cut(s) 1399
MspCI CTTAAG 1 cut(s) 1264
MspI CCGG 9 cut(s) 125, 409, 435, 979, 1391, 1538, 1596, 1787, 1879
MspR9I CCNGG 4 cut(s) 126, 1596, 1597, 1880
Mva1269I GAATGC 2 cut(s) 1006, 1567
MvnI CGCG 5 cut(s) 556, 1261, 1421, 1863, 1958
MwoI GCNNNNNNNGC 5 cut(s) 616, 1267, 1564, 1728, 1765
NciI CCSGG 4 cut(s) 126, 1596, 1597, 1880
NdeI CATATG 1 cut(s) 2165
NlaIV GGNNCC 9 cut(s) 568, 1016, 1176, 1355, 1469, 1576, 1690, 1859, 2287
NmuCI GTSAC 1 cut(s) 1639
NsbI TGCGCA 1 cut(s) 362
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 2 cut(s) 509, 1922
PaeI GCATGC 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 263
PagI TCATGA 1 cut(s) 345
PauI GCGCGC 1 cut(s) 1419
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PctI GAATGC 2 cut(s) 1006, 1567
PdmI GAANNNNTTC 2 cut(s) 992, 1322
PfeI GAWTC 4 cut(s) 41, 317, 848, 2024
PleI GAGTC 5 cut(s) 1345, 1467, 1621, 2009, 2272
PpsI GAGTC 5 cut(s) 1345, 1467, 1621, 2009, 2272
Ppu21I YACGTR 1 cut(s) 309
PpuMI RGGWCCY 2 cut(s) 1015, 2286
PshAI GACNNNNGTC 2 cut(s) 118, 1458
Psp5II RGGWCCY 2 cut(s) 1015, 2286
PspEI GGTNACC 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 2052
PspN4I GGNNCC 9 cut(s) 568, 1016, 1176, 1355, 1469, 1576, 1690, 1859, 2287
PspPI GGNCC 8 cut(s) 127, 976, 1015, 1574, 1688, 1858, 2187, 2286
PspPPI RGGWCCY 2 cut(s) 1015, 2286
PspXI VCTCGAGB 1 cut(s) 263
PstI CTGCAG 4 cut(s) 931, 1032, 1724, 2284
PstNI CAGNNNCTG 2 cut(s) 326, 1728
PsuI RGATCY 1 cut(s) 566
PteI GCGCGC 1 cut(s) 1419
PvuII CAGCTG 1 cut(s) 1399
RsaI GTAC 5 cut(s) 920, 1035, 1062, 1332, 1469
RsaNI GTAC 5 cut(s) 919, 1034, 1061, 1331, 1468
SaqAI TTAA 6 cut(s) 699, 807, 870, 902, 1265, 1272
Sau96I GGNCC 8 cut(s) 127, 976, 1015, 1574, 1688, 1858, 2187, 2286
SchI GAGTC 5 cut(s) 1346, 1468, 1621, 2009, 2273
ScrFI CCNGG 4 cut(s) 126, 1596, 1597, 1880
SfaNI GCATC 3 cut(s) 402, 1432, 1519
SfcI CTRYAG 6 cut(s) 486, 927, 1028, 1366, 1720, 2280
Sfr274I CTCGAG 1 cut(s) 263
SinI GGWCC 4 cut(s) 976, 1015, 1574, 2286
SlaI CTCGAG 1 cut(s) 263
SmaI CCCGGG 1 cut(s) 1597
SmlI CTYRAG 6 cut(s) 263, 907, 1264, 1680, 1822, 2102
SmoI CTYRAG 6 cut(s) 263, 907, 1264, 1680, 1822, 2102
SpeI ACTAGT 1 cut(s) 1636
SphI GCATGC 1 cut(s) 509
SspMI CTAG 8 cut(s) 789, 1019, 1080, 1191, 1637, 1733, 2081, 2290
StyD4I CCNGG 4 cut(s) 124, 1594, 1595, 1878
StyI CCWWGG 2 cut(s) 1018, 1079
TaaI ACNGT 2 cut(s) 180, 1886
TaiI ACGT 2 cut(s) 311, 1674
TaqI TCGA 9 cut(s) 39, 137, 264, 641, 897, 1237, 1603, 2022, 2223
TatI WGTACW 2 cut(s) 1060, 1330
TauI GCSGC 3 cut(s) 188, 538, 1863
TfiI GAWTC 4 cut(s) 41, 317, 848, 2024
Tru1I TTAA 6 cut(s) 699, 807, 870, 902, 1265, 1272
Tru9I TTAA 6 cut(s) 699, 807, 870, 902, 1265, 1272
TscAI CASTG 2 cut(s) 331, 978
TseFI GTSAC 1 cut(s) 1639
Tsp45I GTSAC 1 cut(s) 1639
TspDTI ATGAA 9 cut(s) 26, 334, 369, 462, 743, 764, 1086, 1656, 1902
TspGWI ACGGA 3 cut(s) 154, 906, 1605
TspMI CCCGGG 1 cut(s) 1595
TspRI CASTG 2 cut(s) 331, 978
Vha464I CTTAAG 1 cut(s) 1264
VpaK11BI GGWCC 4 cut(s) 976, 1015, 1574, 2286
XapI RAATTY 1 cut(s) 992
XceI RCATGY 2 cut(s) 509, 1922
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XhoI CTCGAG 1 cut(s) 263
XmaI CCCGGG 1 cut(s) 1595
XmaJI CCTAGG 2 cut(s) 1018, 1079
XmnI GAANNNNTTC 2 cut(s) 992, 1322
XspI CTAG 8 cut(s) 789, 1019, 1080, 1191, 1637, 1733, 2081, 2290
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.