Rroxscaffold_1G00057270
ERF Family

Protein kinase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014690
Physical Location & Seq
Reverse (-)
79232762 .. 79235750
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057270.1

Sequence Viewer

Length: 2313 bp
ATGAAATTAGGTCATCAGCAGAGTGATTTTGATGCATCCCTTGAGGAAATTTTGAATGGGAGTTTCAGGAAATTCACATCTGGTTTATTGCAAAATGATCCGGAGTTATCTCCAGGCCATAGCTCCAATGGGAGTAGTTTTCGGAGGTCGAGTTCTATTATGTCCACACATAGTTTATCAGGCAATTCAGCTTCAAGCAAGTTTGCTCCTTCTAAGAGAAGAGTGTTCAAGGGCTTAAAAGACTATGCTAGGAAACTTGTTGACCTGGAATTGTTCACTCATAGTCTTGAGGATTGGGTTTTGGAGAATTCGTGTGAAGATTCAGCCAATGTGGAGCAGGCTTTTAGTGCTCCCTTTATGATGGATGAATTGCGCAAGTTGGATTTGGCATTGGAGGGTGTTTTGTTTCAGCAACTACTGCGAATGCCATGTTCGAGTTATGGTTCTAGTGGTGCAAATGAAGATGAGTATCTTGCATTGGAGGACTTCCTTCATGCCATAGTGAGTAGTTTGTGGCATGCATTTTGGCATAAAAGAGCACAACCGCCAATGTCTGTATCTTGTCCACGCGGTCTTGGATCAAAATTTTATACTGTGGATAAGGCGATAGCAAGGGGAAGGCTTAATGAGATGAGTGGTTTGGCTTTGATGTCGAAAAATGGGAGTGATCAACAAGTTCAGTGGGATCAGATTATGGAGTTTGCTTTATTTAAGCAAGATATATTGGCAGGTAATGAGTTGAAATTATCGTCTCCCATTATTTGTGAAGCCCTCTTTTATGGTTTTCATATACTTGTTTCTAGGAGTTTGAGCAAGACTAGACCTGCAAATAGCAGTTCTGTTTTTCTTTTGGTTTTAGATTCAAGGTATGGAGGCGTAGTGAAAGTAGGTGGTGATCTTAGCAAACTTGAATTAAACTCAAGTAACCCGTACAAATCTGTGGCTGAATGGATAAAAAATTATGCTGAAGTCAGTGTTTCGCCAGTGGACCGGATATGGAACAAGTTCGGAAATGCAAATTGGGGGGACCTAGGAACCCTACAGATACTTTTGGCGACTTACAACTCCATTGTGCAATGGAATGGACCACCAAGGAAGTCAATTGCCTCGCTAGTTTCAGATCATAGCCTTCGTCTTCAGAAGCGTAGGATGGAGTTCTGCCTCACTGAGAGTGAGAATGGTCTGGTTCCGTACCAACAATCTAGCCACCAGCAAGGACAAGGAGAGATTGTTGAACTTGAGCAAACTAGTAGTCGAGCTTTCAAAAGCAAAGCTTCACGTTTAAATCTCAAGCAGGGTGAGGTATTGCTACTGGAGGATCAACAGCAGGGTCAGAAAACTTTCCAAGTACAGGAGTCTCTGGTAGGAGGGAGCCATTACTTCTATAGTGCTGTTTGTCTAGATTGTCCGACAGAGTTGTTGACTTTATATGTCGGTGCCCATCCATCCAGACTTGAACCATCATGGGAGGATATGAGTCTCTGGTACCAAGTACAAAGACAAACAAAAGTACTAAACATCTTTAAGCATCAAGGAATCACAAGTAACTATTTGCCGGAAATTTTTGCCTCTGGCAGAATTTTGCATTCTGGTCCGTGCCAGAAGCAGGCCCCTGGGGGTCGATGTGATCACCCGTGGTGTGGGACTCCTATACTAGTGACATCTCCAGTCGGTGAACCTGTTTCATATGTTGTTTCTCAAGAAGGCCCTCTTTCTGCTGAGGAAGCAATTCGCTGCTGTCGAGACTGCCTAGCTGCTCTAAGAAGTGCAGCAATGGCGAATGTCCAGCACGGTGATTTGTGTCCAGACAACATTATTCGGGTTGTTGGTGTACAAGGTGCAAGAAACAATGTTCTTTATGTTCCAATATCATGGGGTCGTGCAGTTTTGGAAGACCGGGACAGCCCAGCTATAAACTTGCAATTCTCTTCATCTCATGCACTTCAACATGGGAAACTTTGCCCATCATCAGATGCTGAAAGCCTTGTTTACCTCATGTTGTTCATGTGTGGGGGAACAATGCAGCAGCAGGATTCAATCGAATCTGCATTGCAATGGAGAGAAACAAGCTGGGCAAAGCGCTTGATCCAACAGCAGCTTGGTGAGGTCTCAGCTCTTCTAAAGGCATTTGCTGATTATGTGGATAGCCTTTGTGGAACACCATACCCCGTTGACTACGATATATGGTTGAAAAGATTGAGCAGGGCTGTGGATGGTGGAGCCGATAGAGGTAAAATGATTGAAGAAGTAACTGCTACATTGAGATTGAAGGATGTTGCCGAGTCCTCAGGAACATCTGGAGGTGGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

770

Amino Acids

85.2

Weight (kDa)

6.1

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13040
malus_domestica MD05G1245500.v1.1 MD10G1226800.v1.1
prunus_persica Prupe.4G118400_v2.0.a1
pyrus_communis pycom05g22160 pycom10g19070
rosa_chinensis RchiOBHm_Chr5g0021231
rosa_laevigata RLG00000032597
rosa_multiflora Rmu_sc0003392.1_g000017 Rmu_sc0004847.1_g000001
rosa_roxburghii Rroxscaffold_1G00057270
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154400 Rh5BG154000 Rh5CG167600 Rh5DG154500
rosa_wichuraiana Rw5G013760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 374
Acc36I ACCTGC 2 cut(s) 719, 832
Acc65I GGTACC 1 cut(s) 1485
AccB1I GGYRCC 2 cut(s) 1436, 1485
AccII CGCG 1 cut(s) 570
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 2 cut(s) 545, 570
AclWI GGATC 5 cut(s) 92, 586, 693, 1326, 2080
AcsI RAATTY 6 cut(s) 48, 71, 307, 584, 1560, 1578
AcuI CTGAAG 2 cut(s) 987, 1121
AfaI GTAC 7 cut(s) 932, 1193, 1350, 1487, 1494, 1512, 1833
AfeI AGCGCT 1 cut(s) 2081
AfiI CCNNNNNNNGG 3 cut(s) 1351, 1606, 1640
AhlI ACTAGT 2 cut(s) 1247, 1654
AjnI CCWGG 3 cut(s) 112, 264, 1612
AjuI GAANNNNNNNTTGG 2 cut(s) 1003, 1035
AloI GAACNNNNNNTCC 2 cut(s) 136, 168
AluBI AGCT 9 cut(s) 123, 191, 1259, 1274, 1754, 1910, 2070, 2098, 2114
AluI AGCT 9 cut(s) 123, 191, 1259, 1274, 1754, 1910, 2070, 2098, 2114
Alw21I GWGCWC 2 cut(s) 352, 541
Alw26I GTCTC 5 cut(s) 756, 1362, 1484, 1737, 2113
AlwI GGATC 5 cut(s) 92, 586, 693, 1326, 2080
AlwNI CAGNNNCTG 1 cut(s) 1976
Aor13HI TCCGGA 1 cut(s) 100
Aor51HI AGCGCT 1 cut(s) 2081
AoxI GGCC 3 cut(s) 115, 1608, 1705
ApeKI GCWGC 6 cut(s) 1734, 1754, 1769, 2023, 2026, 2095
ApoI RAATTY 6 cut(s) 48, 71, 307, 584, 1560, 1578
ArsI GACNNNNNNTTYG 2 cut(s) 1237, 1269
Asp700I GAANNNNTTC 3 cut(s) 1004, 1340, 1728
Asp718I GGTACC 1 cut(s) 1485
AspA2I CCTAGG 1 cut(s) 1030
AspLEI GCGC 2 cut(s) 375, 2082
AspS9I GGNCC 6 cut(s) 988, 1027, 1085, 1592, 1609, 1706
AsuC2I CCSGG 1 cut(s) 1898
AsuHPI GGTGA 6 cut(s) 905, 1310, 1622, 1685, 1805, 2114
AvaII GGWCC 4 cut(s) 988, 1027, 1085, 1592
AvrII CCTAGG 1 cut(s) 1030
AxyI CCTNAGG 1 cut(s) 2287
BaeGI GKGCMC 1 cut(s) 1441
BanI GGYRCC 2 cut(s) 1436, 1485
BbsI GAAGAC 2 cut(s) 1127, 1899
Bbv12I GWGCWC 2 cut(s) 352, 541
BbvCI CCTCAGC 1 cut(s) 1719
BbvI GCAGC 6 cut(s) 1721, 1741, 1781, 2035, 2038, 2107
BccI CCATC 7 cut(s) 355, 1144, 1449, 1453, 1468, 1972, 2207
BciT130I CCWGG 3 cut(s) 114, 266, 1614
BclI TGATCA 2 cut(s) 667, 1627
BcnI CCSGG 1 cut(s) 1898
BcoDI GTCTC 5 cut(s) 756, 1362, 1484, 1737, 2113
BcuI ACTAGT 2 cut(s) 1247, 1654
BfmI CTRYAG 2 cut(s) 1040, 1384
BfoI RGCGCY 1 cut(s) 2083
BfuAI ACCTGC 2 cut(s) 719, 832
BisI GCNGC 6 cut(s) 1735, 1755, 1770, 2024, 2027, 2096
BlnI CCTAGG 1 cut(s) 1030
BlsI GCNGC 6 cut(s) 1736, 1756, 1771, 2025, 2028, 2097
BmcAI AGTACT 1 cut(s) 1512
Bme1390I CCNGG 4 cut(s) 114, 266, 1614, 1898
Bme18I GGWCC 4 cut(s) 988, 1027, 1085, 1592
BmgT120I GGNCC 6 cut(s) 988, 1027, 1085, 1592, 1609, 1706
BmiI GGNNCC 8 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221
BmrFI CCNGG 4 cut(s) 114, 266, 1614, 1898
BmsI GCATC 4 cut(s) 22, 44, 1537, 1963
BpiI GAAGAC 2 cut(s) 1127, 1899
BpmI CTGGAG 3 cut(s) 96, 1334, 1650
Bpu10I CCTNAGC 1 cut(s) 1719
BpuEI CTTGAG 6 cut(s) 62, 308, 904, 1259, 1274, 1683
BpuMI CCSGG 1 cut(s) 1898
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 2113
BsaJI CCNNGG 5 cut(s) 1030, 1091, 1612, 1613, 1634
BsaWI WCCGGW 2 cut(s) 100, 990
BsaXI ACNNNNNCTCC 8 cut(s) 136, 166, 296, 326, 864, 894, 1215, 1245
Bsc4I CCNNNNNNNGG 3 cut(s) 1351, 1606, 1640
Bse1I ACTGG 3 cut(s) 983, 1317, 1667
Bse21I CCTNAGG 1 cut(s) 2287
Bse3DI GCAATG 4 cut(s) 1082, 1779, 2048, 2060
Bse8I GATNNNNATC 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 3 cut(s) 114, 266, 1614
BseDI CCNNGG 5 cut(s) 1030, 1091, 1612, 1613, 1634
BseGI GGATG 7 cut(s) 35, 370, 1155, 1441, 1445, 2218, 2278
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 3 cut(s) 1351, 1606, 1640
BseMI GCAATG 4 cut(s) 1082, 1779, 2048, 2060
BseMII CTCAG 4 cut(s) 1158, 1710, 2124, 2301
BseNI ACTGG 3 cut(s) 983, 1317, 1667
BseSI GKGCMC 1 cut(s) 1441
BseXI GCAGC 6 cut(s) 1721, 1741, 1781, 2035, 2038, 2107
BseYI CCCAGC 2 cut(s) 1906, 2070
BsgI GTGCAG 2 cut(s) 1788, 1902
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 3 cut(s) 117, 1610, 1707
BshNI GGYRCC 2 cut(s) 1436, 1485
BsiHKAI GWGCWC 2 cut(s) 352, 541
BsiSI CCGG 4 cut(s) 101, 991, 1556, 1897
BslFI GGGAC 3 cut(s) 1040, 1657, 1913
BslI CCNNNNNNNGG 3 cut(s) 1351, 1606, 1640
BsmAI GTCTC 5 cut(s) 756, 1362, 1484, 1737, 2113
BsmBI CGTCTC 1 cut(s) 756
BsmFI GGGAC 3 cut(s) 1040, 1657, 1913
BsmI GAATGC 2 cut(s) 429, 1585
BsnI GGCC 3 cut(s) 117, 1610, 1707
Bso31I GGTCTC 1 cut(s) 2113
Bsp1286I GDGCHC 3 cut(s) 352, 541, 1441
Bsp13I TCCGGA 1 cut(s) 100
Bsp1407I TGTACA 1 cut(s) 1831
Bsp143I GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
BspACI CCGC 2 cut(s) 545, 570
BspANI GGCC 3 cut(s) 117, 1610, 1707
BspCNI CTCAG 4 cut(s) 1159, 1711, 2123, 2300
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 8 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221
BspMI ACCTGC 2 cut(s) 719, 832
BspPI GGATC 5 cut(s) 92, 586, 693, 1326, 2080
BspQI GCTCTTC 1 cut(s) 2121
BspT107I GGYRCC 2 cut(s) 1436, 1485
BspTNI GGTCTC 1 cut(s) 2113
BsrDI GCAATG 4 cut(s) 1082, 1779, 2048, 2060
BsrGI TGTACA 1 cut(s) 1831
BsrI ACTGG 3 cut(s) 983, 1317, 1667
BssECI CCNNGG 5 cut(s) 1030, 1091, 1612, 1613, 1634
BssMI GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
BssT1I CCWWGG 2 cut(s) 1030, 1091
Bst2UI CCWGG 3 cut(s) 114, 266, 1614
Bst4CI ACNGT 2 cut(s) 595, 1793
Bst6I CTCTTC 3 cut(s) 214, 1933, 2121
BstAPI GCANNNNNTGC 1 cut(s) 418
BstAUI TGTACA 1 cut(s) 1831
BstC8I GCNNGC 3 cut(s) 339, 519, 1608
BstDEI CTNAG 7 cut(s) 213, 899, 1167, 1719, 1760, 2110, 2287
BstDSI CCRYGG 1 cut(s) 1634
BstF5I GGATG 7 cut(s) 35, 370, 1155, 1441, 1445, 2218, 2278
BstFNI CGCG 1 cut(s) 570
BstH2I RGCGCY 1 cut(s) 2083
BstHHI GCGC 2 cut(s) 375, 2082
BstKTI GATC 9 cut(s) 100, 581, 670, 688, 898, 1123, 1321, 1630, 2088
BstMAI GTCTC 5 cut(s) 756, 1362, 1484, 1737, 2113
BstMBI GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
BstMWI GCNNNNNNNGC 4 cut(s) 347, 418, 1724, 1775
BstNI CCWGG 3 cut(s) 114, 266, 1614
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 4 cut(s) 112, 264, 1612, 1896
BstSFI CTRYAG 2 cut(s) 1040, 1384
BstSLI GKGCMC 1 cut(s) 1441
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 6 cut(s) 1721, 1741, 1781, 2035, 2038, 2107
BstV2I GAAGAC 2 cut(s) 1127, 1899
BstXI CCANNNNNNTGG 1 cut(s) 1872
Bsu36I CCTNAGG 1 cut(s) 2287
BsuRI GGCC 3 cut(s) 117, 1610, 1707
BtgI CCRYGG 1 cut(s) 1634
BtsCI GGATG 7 cut(s) 35, 370, 1155, 1441, 1445, 2218, 2278
BtsIMutI CAGTG 4 cut(s) 686, 979, 990, 1164
BveI ACCTGC 2 cut(s) 719, 832
Cac8I GCNNGC 3 cut(s) 339, 519, 1608
CaiI CAGNNNCTG 1 cut(s) 1976
CfoI GCGC 2 cut(s) 375, 2082
Cfr13I GGNCC 6 cut(s) 988, 1027, 1085, 1592, 1609, 1706
Csp6I GTAC 7 cut(s) 931, 1192, 1349, 1486, 1493, 1511, 1832
CspCI CAANNNNNGTGG 2 cut(s) 662, 697
CviAII CATG 9 cut(s) 429, 494, 518, 1464, 1872, 1937, 1949, 1996, 2005
CviQI GTAC 7 cut(s) 931, 1192, 1349, 1486, 1493, 1511, 1832
DdeI CTNAG 7 cut(s) 213, 899, 1167, 1719, 1760, 2110, 2287
DpnI GATC 9 cut(s) 99, 580, 669, 687, 897, 1122, 1320, 1629, 2087
DpnII GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
DraI TTTAAA 1 cut(s) 1284
Eam1104I CTCTTC 3 cut(s) 214, 1933, 2121
EarI CTCTTC 3 cut(s) 214, 1933, 2121
Eco130I CCWWGG 2 cut(s) 1030, 1091
Eco31I GGTCTC 1 cut(s) 2113
Eco47I GGWCC 4 cut(s) 988, 1027, 1085, 1592
Eco47III AGCGCT 1 cut(s) 2081
Eco57I CTGAAG 2 cut(s) 987, 1121
Eco81I CCTNAGG 1 cut(s) 2287
EcoO109I RGGNCCY 3 cut(s) 1027, 1609, 1706
EcoRI GAATTC 1 cut(s) 307
EcoRII CCWGG 3 cut(s) 112, 264, 1612
EcoT14I CCWWGG 2 cut(s) 1030, 1091
EcoT22I ATGCAT 2 cut(s) 37, 523
ErhI CCWWGG 2 cut(s) 1030, 1091
Esp3I CGTCTC 1 cut(s) 756
FaeI CATG 9 cut(s) 432, 497, 521, 1467, 1875, 1940, 1952, 1999, 2008
FalI AAGNNNNNCTT 4 cut(s) 1258, 1290, 1695, 1727
FaqI GGGAC 3 cut(s) 1040, 1657, 1913
FatI CATG 9 cut(s) 428, 493, 517, 1463, 1871, 1936, 1948, 1995, 2004
FauNDI CATATG 1 cut(s) 1687
FbaI TGATCA 2 cut(s) 667, 1627
Fnu4HI GCNGC 6 cut(s) 1735, 1755, 1770, 2024, 2027, 2096
FokI GGATG 7 cut(s) 22, 377, 1162, 1428, 1432, 2225, 2285
Fsp4HI GCNGC 6 cut(s) 1735, 1755, 1770, 2024, 2027, 2096
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 2 cut(s) 374, 2081
GluI GCNGC 6 cut(s) 1735, 1755, 1770, 2024, 2027, 2096
GsaI CCCAGC 2 cut(s) 1910, 2074
GsuI CTGGAG 3 cut(s) 96, 1334, 1650
HaeII RGCGCY 1 cut(s) 2083
HaeIII GGCC 3 cut(s) 117, 1610, 1707
HapII CCGG 4 cut(s) 101, 991, 1556, 1897
HhaI GCGC 2 cut(s) 375, 2082
Hin1II CATG 9 cut(s) 432, 497, 521, 1467, 1875, 1940, 1952, 1999, 2008
Hin6I GCGC 2 cut(s) 373, 2080
HinP1I GCGC 2 cut(s) 373, 2080
HincII GTYRAC 3 cut(s) 262, 1422, 2173
HindII GTYRAC 3 cut(s) 262, 1422, 2173
HindIII AAGCTT 1 cut(s) 1272
HinfI GANTC 9 cut(s) 320, 860, 1355, 1477, 1536, 1645, 2033, 2042, 2282
HpaII CCGG 4 cut(s) 101, 991, 1556, 1897
HphI GGTGA 6 cut(s) 905, 1310, 1622, 1685, 1805, 2114
Hpy188I TCNGA 8 cut(s) 144, 690, 1010, 1120, 1140, 1335, 1410, 1972
HpyAV CCTTC 6 cut(s) 219, 500, 612, 1139, 1697, 2263
HpyCH4III ACNGT 2 cut(s) 595, 1793
HpyCH4IV ACGT 1 cut(s) 1279
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 418, 1724, 1775
HpyF3I CTNAG 7 cut(s) 213, 899, 1167, 1719, 1760, 2110, 2287
HpySE526I ACGT 1 cut(s) 1279
Hsp92II CATG 9 cut(s) 432, 497, 521, 1467, 1875, 1940, 1952, 1999, 2008
HspAI GCGC 2 cut(s) 373, 2080
Kpn2I TCCGGA 1 cut(s) 100
KpnI GGTACC 1 cut(s) 1489
Ksp22I TGATCA 2 cut(s) 667, 1627
Kzo9I GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
LguI GCTCTTC 1 cut(s) 2121
LmnI GCTCC 6 cut(s) 128, 211, 334, 355, 1371, 2219
Lsp1109I GCAGC 6 cut(s) 1721, 1741, 1781, 2035, 2038, 2107
LweI GCATC 4 cut(s) 22, 44, 1537, 1963
MaeII ACGT 1 cut(s) 1279
MaeIII GTNAC 4 cut(s) 923, 1544, 1657, 2248
MalI GATC 9 cut(s) 99, 580, 669, 687, 897, 1122, 1320, 1629, 2087
MboI GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
MboII GAAGA 8 cut(s) 231, 329, 473, 1127, 1904, 1920, 2108, 2255
MfeI CAATTG 1 cut(s) 1101
MhlI GDGCHC 3 cut(s) 352, 541, 1441
MlyI GAGTC 4 cut(s) 1364, 1486, 1639, 2291
MmeI TCCRAC 3 cut(s) 360, 1433, 2113
Mph1103I ATGCAT 2 cut(s) 37, 523
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 3 cut(s) 1004, 1340, 1728
MseI TTAA 6 cut(s) 236, 624, 711, 914, 1283, 1524
MslI CAYNNNNRTG 1 cut(s) 2053
MspI CCGG 4 cut(s) 101, 991, 1556, 1897
MspR9I CCNGG 4 cut(s) 114, 266, 1614, 1898
MunI CAATTG 1 cut(s) 1101
Mva1269I GAATGC 2 cut(s) 429, 1585
MvaI CCWGG 3 cut(s) 114, 266, 1614
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 4 cut(s) 347, 418, 1724, 1775
NciI CCSGG 1 cut(s) 1898
NdeI CATATG 1 cut(s) 1687
NdeII GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
NlaIII CATG 9 cut(s) 432, 497, 521, 1467, 1875, 1940, 1952, 1999, 2008
NlaIV GGNNCC 8 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221
NmeAIII GCCGAG 1 cut(s) 2305
NmuCI GTSAC 1 cut(s) 1657
NsbI TGCGCA 1 cut(s) 374
NsiI ATGCAT 2 cut(s) 37, 523
NspI RCATGY 1 cut(s) 521
PaeI GCATGC 1 cut(s) 521
PasI CCCWGGG 1 cut(s) 1613
PciSI GCTCTTC 1 cut(s) 2121
PcsI WCGNNNNNNNCGW 1 cut(s) 1738
PctI GAATGC 2 cut(s) 429, 1585
PdmI GAANNNNTTC 3 cut(s) 1004, 1340, 1728
PfeI GAWTC 5 cut(s) 320, 860, 1536, 2033, 2042
PkrI GCNGC 6 cut(s) 1736, 1756, 1771, 2025, 2028, 2097
PleI GAGTC 4 cut(s) 1363, 1485, 1639, 2290
PpsI GAGTC 4 cut(s) 1363, 1485, 1639, 2290
PpuMI RGGWCCY 1 cut(s) 1027
Psp5II RGGWCCY 1 cut(s) 1027
Psp6I CCWGG 3 cut(s) 112, 264, 1612
PspFI CCCAGC 2 cut(s) 1906, 2070
PspGI CCWGG 3 cut(s) 112, 264, 1612
PspN4I GGNNCC 8 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221
PspPI GGNCC 6 cut(s) 988, 1027, 1085, 1592, 1609, 1706
PspPPI RGGWCCY 1 cut(s) 1027
PstNI CAGNNNCTG 1 cut(s) 1976
RsaI GTAC 7 cut(s) 932, 1193, 1350, 1487, 1494, 1512, 1833
RsaNI GTAC 7 cut(s) 931, 1192, 1349, 1486, 1493, 1511, 1832
RseI CAYNNNNRTG 1 cut(s) 2053
SapI GCTCTTC 1 cut(s) 2121
SaqAI TTAA 6 cut(s) 236, 624, 711, 914, 1283, 1524
SatI GCNGC 6 cut(s) 1735, 1755, 1770, 2024, 2027, 2096
Sau3AI GATC 9 cut(s) 97, 578, 667, 685, 895, 1120, 1318, 1627, 2085
Sau96I GGNCC 6 cut(s) 988, 1027, 1085, 1592, 1609, 1706
ScaI AGTACT 1 cut(s) 1512
SchI GAGTC 4 cut(s) 1364, 1486, 1639, 2291
ScrFI CCNGG 4 cut(s) 114, 266, 1614, 1898
SduI GDGCHC 3 cut(s) 352, 541, 1441
SfaNI GCATC 4 cut(s) 22, 44, 1537, 1963
SfcI CTRYAG 2 cut(s) 1040, 1384
SinI GGWCC 4 cut(s) 988, 1027, 1085, 1592
SmiMI CAYNNNNRTG 1 cut(s) 2053
SmlI CTYRAG 6 cut(s) 41, 287, 919, 1238, 1289, 1698
SmoI CTYRAG 6 cut(s) 41, 287, 919, 1238, 1289, 1698
SpeI ACTAGT 2 cut(s) 1247, 1654
SphI GCATGC 1 cut(s) 521
SsiI CCGC 2 cut(s) 545, 570
StyD4I CCNGG 4 cut(s) 112, 264, 1612, 1896
StyI CCWWGG 2 cut(s) 1030, 1091
TaaI ACNGT 2 cut(s) 595, 1793
TaiI ACGT 1 cut(s) 1282
TaqI TCGA 7 cut(s) 149, 434, 653, 1255, 1621, 1741, 2040
TatI WGTACW 4 cut(s) 1348, 1492, 1510, 1831
TfiI GAWTC 5 cut(s) 320, 860, 1536, 2033, 2042
Tru1I TTAA 6 cut(s) 236, 624, 711, 914, 1283, 1524
Tru9I TTAA 6 cut(s) 236, 624, 711, 914, 1283, 1524
TscAI CASTG 4 cut(s) 686, 979, 990, 1171
TseFI GTSAC 1 cut(s) 1657
TseI GCWGC 6 cut(s) 1734, 1754, 1769, 2023, 2026, 2095
Tsp45I GTSAC 1 cut(s) 1657
TspDTI ATGAA 8 cut(s) 17, 381, 474, 482, 776, 1674, 1920, 1993
TspGWI ACGGA 2 cut(s) 1179, 1584
TspRI CASTG 4 cut(s) 686, 979, 990, 1171
VpaK11BI GGWCC 4 cut(s) 988, 1027, 1085, 1592
XapI RAATTY 6 cut(s) 48, 71, 307, 584, 1560, 1578
XbaI TCTAGA 1 cut(s) 1399
XceI RCATGY 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 2 cut(s) 125, 2096
XmaJI CCTAGG 1 cut(s) 1030
XmnI GAANNNNTTC 3 cut(s) 1004, 1340, 1728
ZrmI AGTACT 1 cut(s) 1512
Zsp2I ATGCAT 2 cut(s) 37, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.