Rmu_sc0004847.1_g000001
ERF Family

Protein kinase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004847.1
OG0014690
Physical Location & Seq
Forward (+)
301 .. 6429
6129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004847.1_g000001.1.cds

Sequence Viewer

Length: 3432 bp
atgaaattaggtcatcagcagagtgattttgatgcatcccttgaggaaattttgaatgggagtttcaggaaatttacatctggtttattgcaaaacgatccggagttatctccaggccatagctccaatgggagtagttttcggaggtcgagttctattatgtccacacatagtttatcaggcaattcagcttcaagcaagtttgctccttctaagagaagagtgttcaagggcttaaaagactattctaggaaacttgttgacctggaattgttcactcaaagtcttgaggattgggttttggagaattcgtgtgaagattcagcgaatggggagcaggcttttagtgctccctttatgatggatgaattgcgcaagttggatttggcattggagggtgttttgtttcagcaactactgcgaatgccatgttcgagttatggttctagtggtgcaaatgaagatgagtatcttgcattggaggacttccttcatgccatagtgagtggtttgtggcatgcattttggcataaaagagcgcaaccgccaatgtctgtatcttgtccacgcggtcttggatcaaaattttatactgtggataaggcgatagcaaggggaaggcttaatgagatgagtggtttggctttgatgtcgaaaaatgggagtgatcaacaagttcagtgggatcagattatgcagtttgctttatttaagcaagatatattggcaggtaatgagttgaaattatcgtcttccattatttgtgaagccctcttttatggttttcatatacttgtttctaggagtttgagcaagaccagacctgcaaatagcagttctgtttttcttttggttttagattcaaggtatggaggcgtagtgaaagtaggtggtgatcttagcaaacttgaattaaactctagtaacccgtacaaatctgtggctgaatggataaaaaattatgctgaagtcagtgtttcgccagtggaccggatatggaacaagttcggaaatgcaaattggggggacctaggaaccctacagatacttttggcgacttacaactccattgtgcaatggaatggaccaccaaggaagtcaattgcctcgttagtttcagatcatagccttcgtcttcagaagcgtaggatggagttctgcctcactgagaatgagaatggtctggttccgtaccaacaatctagccaccagcaaggacaaggagagattgttgaacttgagcaaactagtagtcgagctttcaaaagcaaagcttcacgtttaaatctcaagcagggtgaggtattgctactggaggatcaacagcagggtcagaaaactttccaagtacaggagtctctggtaggagggagccattacttctatagtgctgtttgtctagattgtccgacagagttgttgactttatatgtcggtgcccatccatccagacttgaaccatcttgggaggatatgagtctctggtaccaagtacaaagacaaacaaaagtactaaacatctttaagcatcaaggaatcacaagtaactatttgccggaaatttttgcctctggcagaattttgcattctggtccgtgccagaagcaggcccccgggggtcgatgtgatcacccgtggtgtggaactcctatactagtgacatctccagtcggtgaacctgtttcatatgttgtttctcaagaaggccctctttctgctgaggaagcaattcgctgctgtcgagactgcctagctgctctaagaagtgcagcaatggcgaatgtccagcatggtgatttgtgtccagacaacattattcgggttgttggtatacaaggtgcaagaaacaatgttctctatgttccaatatcatggggtcgtgcagttttggaagaccgggacagcccagctataaacttgcaattctcttcatctcatgcacttcaacatgggaaactttgcccatcatcagatgcagaaagccttgtttacctcatgttgttcatgtgtgggggaacaatgcagcagcaggattcaatcgaatctgcattgcaatggagagaaacaagctgggcaaagcgcttgatccaacagcagcttggtgaggtctcagctcttctaaaggcatttgctgattatgtggatagcctttgtggaacaccataccccgttgactacgatatatggttgaaaagattgagcagggctgtggatggtggagccgatagaggtaaaatgattgaagaagtaactgctacattgagattgaaggatgtagccgagtcctcaggaacatctggaggtgtctgtctacatagtactgatgctctgagcactgctagtgccaatatggagaatcacagcaaaacccagcttcaaagtttcctcaaacatcttcctcctgttgagttctgctgtgtttatgggtcatcactacacccaaatcgtgacaatgccaagtcaacaatggtggattgcattcttggagtatcagatcccctacaatggcattctgagaatctgaaattgaacaaggatcactatgcctcttggatgtcgctgctcggtggggccagactggttactaatgttgcagattacataggtgtgggagtgcacttcaatccttttgttagttggaacgacaagatgttcaagtatggggttgtccgcatgcatgacttggtgcaggacatatcaaattgggagaggttctacttgagtggccggttacaaaagccagttcaaataatttctgataatttgaatgttgcaaatgtaaactcggttaatttgagggctgcaatagctgctgctctccttcttttgccatccaagttcactgaggaggatctgtatgccaaaatatgtagcctctcatatacgggtgacttacgtatgctctttgcagaagacagaaataaggtgaagaaaatagtacaagggcaatttgagttgtttcggtcaatgtataagccatttattcaagagtatgagactaatgagttgttgagatactcattgtctggaaatcctcaaccagtcgtttctcaggattgtgatttatcagcggctcgatcccttgtttcttctctgcccccactggtcagaagccaaatgggagtgaagcttggagagaagaaaaggctgtgtgactctggtatgaacttcaactcctacacattctctgtgaagacaaggtttattgcattcatcatacttcaatttaattcaatagcaggtcgagttattaacgaagttgtgattggctcaagacaagaggctgctcaatgcatgcagacaattttgaagcgaaaggtaatggtttcaagtgcaagacaggctgtctctggtctgctggctgttggtggtgttaatggcatgagatatcttggcgctaaaatgcgcaaggcttggaattcgtggagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1143

Amino Acids

127.1

Weight (kDa)

8.14

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13040
malus_domestica MD05G1245500.v1.1 MD10G1226800.v1.1
prunus_persica Prupe.4G118400_v2.0.a1
pyrus_communis pycom05g22160 pycom10g19070
rosa_chinensis RchiOBHm_Chr5g0021231
rosa_laevigata RLG00000032597
rosa_multiflora Rmu_sc0003392.1_g000017 Rmu_sc0004847.1_g000001
rosa_roxburghii Rroxscaffold_1G00057270
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154400 Rh5BG154000 Rh5CG167600 Rh5DG154500
rosa_wichuraiana Rw5G013760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 374, 3407
Acc36I ACCTGC 3 cut(s) 719, 832, 3230
Acc65I GGTACC 1 cut(s) 1485
AccB1I GGYRCC 2 cut(s) 1436, 1485
AccI GTMKAC 2 cut(s) 1831, 2311
AccII CGCG 1 cut(s) 570
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 4 cut(s) 545, 570, 2671, 3071
AclWI GGATC 9 cut(s) 92, 586, 693, 1326, 2080, 2489, 2544, 2859, 3072
AcoI YGGCCR 1 cut(s) 2725
AcsI RAATTY 7 cut(s) 48, 71, 307, 584, 1560, 1578, 3418
AcuI CTGAAG 2 cut(s) 987, 1121
AfaI GTAC 8 cut(s) 932, 1193, 1350, 1487, 1494, 1512, 2320, 2940
AfeI AGCGCT 1 cut(s) 2081
AfiI CCNNNNNNNGG 4 cut(s) 1351, 1606, 1640, 2505
AhdI GACNNNNNGTC 1 cut(s) 3344
AhlI ACTAGT 2 cut(s) 1247, 1654
AjnI CCWGG 2 cut(s) 112, 264
AjuI GAANNNNNNNTTGG 4 cut(s) 1003, 1035, 3249, 3281
AloI GAACNNNNNNTCC 4 cut(s) 136, 168, 3158, 3190
Alw21I GWGCWC 3 cut(s) 352, 2336, 2619
Alw26I GTCTC 6 cut(s) 1362, 1484, 1737, 2113, 2990, 3352
Alw44I GTGCAC 1 cut(s) 2615
AlwI GGATC 9 cut(s) 92, 586, 693, 1326, 2080, 2489, 2544, 2859, 3072
Ama87I CYCGRG 1 cut(s) 1613
Aor13HI TCCGGA 1 cut(s) 100
Aor51HI AGCGCT 1 cut(s) 2081
AoxI GGCC 5 cut(s) 115, 1608, 1705, 2571, 2725
ApaLI GTGCAC 1 cut(s) 2615
ApoI RAATTY 7 cut(s) 48, 71, 307, 584, 1560, 1578, 3418
ArsI GACNNNNNNTTYG 2 cut(s) 1237, 1269
Asp700I GAANNNNTTC 3 cut(s) 1004, 1340, 1728
Asp718I GGTACC 1 cut(s) 1485
AspA2I CCTAGG 1 cut(s) 1030
AspLEI GCGC 5 cut(s) 375, 541, 2082, 3398, 3408
AspS9I GGNCC 7 cut(s) 988, 1027, 1085, 1592, 1609, 1706, 2571
AsuC2I CCSGG 3 cut(s) 1614, 1615, 1898
AsuHPI GGTGA 8 cut(s) 905, 1310, 1622, 1685, 1805, 2114, 2900, 2938
AvaI CYCGRG 1 cut(s) 1613
AvaII GGWCC 4 cut(s) 988, 1027, 1085, 1592
AvrII CCTAGG 1 cut(s) 1030
AxyI CCTNAGG 1 cut(s) 2287
BaeGI GKGCMC 2 cut(s) 1441, 2619
BanI GGYRCC 2 cut(s) 1436, 1485
BbsI GAAGAC 5 cut(s) 744, 1127, 1899, 2919, 3200
Bbv12I GWGCWC 3 cut(s) 352, 2336, 2619
BbvCI CCTCAGC 1 cut(s) 1719
BccI CCATC 8 cut(s) 355, 1144, 1449, 1453, 1468, 1972, 2207, 2839
BciT130I CCWGG 2 cut(s) 114, 266
BclI TGATCA 2 cut(s) 667, 1627
BcnI CCSGG 3 cut(s) 1614, 1615, 1898
BcoDI GTCTC 6 cut(s) 1362, 1484, 1737, 2113, 2990, 3352
BcuI ACTAGT 2 cut(s) 1247, 1654
BfmI CTRYAG 2 cut(s) 1040, 1384
BfoI RGCGCY 2 cut(s) 2083, 3399
BfuAI ACCTGC 3 cut(s) 719, 832, 3230
BlnI CCTAGG 1 cut(s) 1030
BmcAI AGTACT 2 cut(s) 1512, 2320
Bme1390I CCNGG 5 cut(s) 114, 266, 1614, 1615, 1898
Bme18I GGWCC 4 cut(s) 988, 1027, 1085, 1592
BmeRI GACNNNNNGTC 1 cut(s) 3344
BmeT110I CYCGRG 1 cut(s) 1613
BmgT120I GGNCC 7 cut(s) 988, 1027, 1085, 1592, 1609, 1706, 2571
BmiI GGNNCC 9 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221, 2572
BmrFI CCNGG 5 cut(s) 114, 266, 1614, 1615, 1898
BmsI GCATC 5 cut(s) 22, 44, 1537, 1963, 2314
BpiI GAAGAC 5 cut(s) 744, 1127, 1899, 2919, 3200
BpmI CTGGAG 4 cut(s) 96, 1334, 1650, 2319
Bpu10I CCTNAGC 1 cut(s) 1719
BpuEI CTTGAG 7 cut(s) 62, 308, 1259, 1274, 1683, 2740, 3256
BpuMI CCSGG 3 cut(s) 1614, 1615, 1898
BsaAI YACGTR 1 cut(s) 2897
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 2113
BsaJI CCNNGG 6 cut(s) 1030, 1091, 1612, 1613, 1614, 1634
BsaWI WCCGGW 2 cut(s) 100, 990
Bsc4I CCNNNNNNNGG 4 cut(s) 1351, 1606, 1640, 2505
Bse118I RCCGGY 1 cut(s) 2727
Bse1I ACTGG 7 cut(s) 983, 1317, 1667, 2583, 2741, 3041, 3108
Bse21I CCTNAGG 1 cut(s) 2287
Bse3DI GCAATG 4 cut(s) 1082, 1779, 2048, 2060
Bse8I GATNNNNATC 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 2 cut(s) 114, 266
BseDI CCNNGG 6 cut(s) 1030, 1091, 1612, 1613, 1614, 1634
BseGI GGATG 9 cut(s) 35, 370, 1155, 1441, 1445, 2218, 2278, 2559, 2831
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 4 cut(s) 1351, 1606, 1640, 2505
BseMI GCAATG 4 cut(s) 1082, 1779, 2048, 2060
BseMII CTCAG 8 cut(s) 1158, 1710, 2124, 2301, 2321, 2505, 2835, 3065
BseNI ACTGG 7 cut(s) 983, 1317, 1667, 2583, 2741, 3041, 3108
BseRI GAGGAG 1 cut(s) 2861
BseSI GKGCMC 2 cut(s) 1441, 2619
BseYI CCCAGC 3 cut(s) 1906, 2070, 2370
BsgI GTGCAG 3 cut(s) 1788, 1902, 2708
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 5 cut(s) 117, 1610, 1707, 2573, 2727
BshNI GGYRCC 2 cut(s) 1436, 1485
BsiHKAI GWGCWC 3 cut(s) 352, 2336, 2619
BsiHKCI CYCGRG 1 cut(s) 1613
BsiSI CCGG 6 cut(s) 101, 991, 1556, 1614, 1897, 2728
BslFI GGGAC 2 cut(s) 1040, 1913
BslI CCNNNNNNNGG 4 cut(s) 1351, 1606, 1640, 2505
BsmAI GTCTC 6 cut(s) 1362, 1484, 1737, 2113, 2990, 3352
BsmFI GGGAC 2 cut(s) 1040, 1913
BsmI GAATGC 5 cut(s) 429, 1585, 2478, 2509, 3209
BsnI GGCC 5 cut(s) 117, 1610, 1707, 2573, 2727
Bso31I GGTCTC 1 cut(s) 2113
BsoBI CYCGRG 1 cut(s) 1613
Bsp1286I GDGCHC 4 cut(s) 352, 1441, 2336, 2619
Bsp13I TCCGGA 1 cut(s) 100
BspACI CCGC 4 cut(s) 545, 570, 2671, 3071
BspANI GGCC 5 cut(s) 117, 1610, 1707, 2573, 2727
BspCNI CTCAG 8 cut(s) 1159, 1711, 2123, 2300, 2322, 2506, 2836, 3064
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 9 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221, 2572
BspMI ACCTGC 3 cut(s) 719, 832, 3230
BspPI GGATC 9 cut(s) 92, 586, 693, 1326, 2080, 2489, 2544, 2859, 3072
BspQI GCTCTTC 1 cut(s) 2121
BspT107I GGYRCC 2 cut(s) 1436, 1485
BspTNI GGTCTC 1 cut(s) 2113
BsrDI GCAATG 4 cut(s) 1082, 1779, 2048, 2060
BsrFI RCCGGY 1 cut(s) 2727
BsrI ACTGG 7 cut(s) 983, 1317, 1667, 2583, 2741, 3041, 3108
BssAI RCCGGY 1 cut(s) 2727
BssECI CCNNGG 6 cut(s) 1030, 1091, 1612, 1613, 1614, 1634
BssNAI GTATAC 1 cut(s) 1832
BssT1I CCWWGG 2 cut(s) 1030, 1091
Bst1107I GTATAC 1 cut(s) 1832
Bst2UI CCWGG 2 cut(s) 114, 266
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 3 cut(s) 214, 1933, 2121
BstAPI GCANNNNNTGC 2 cut(s) 418, 2810
BstBAI YACGTR 1 cut(s) 2897
BstC8I GCNNGC 6 cut(s) 339, 519, 1608, 2675, 3296, 3360
BstDSI CCRYGG 1 cut(s) 1634
BstF5I GGATG 9 cut(s) 35, 370, 1155, 1441, 1445, 2218, 2278, 2559, 2831
BstFNI CGCG 1 cut(s) 570
BstH2I RGCGCY 2 cut(s) 2083, 3399
BstHHI GCGC 5 cut(s) 375, 541, 2082, 3398, 3408
BstMAI GTCTC 6 cut(s) 1362, 1484, 1737, 2113, 2990, 3352
BstMWI GCNNNNNNNGC 7 cut(s) 347, 418, 1724, 1775, 2807, 2810, 3341
BstNI CCWGG 2 cut(s) 114, 266
BstNSI RCATGY 3 cut(s) 521, 2677, 3298
BstSCI CCNGG 5 cut(s) 112, 264, 1612, 1613, 1896
BstSFI CTRYAG 2 cut(s) 1040, 1384
BstSLI GKGCMC 2 cut(s) 1441, 2619
BstSNI TACGTA 1 cut(s) 2897
BstUI CGCG 1 cut(s) 570
BstV2I GAAGAC 5 cut(s) 744, 1127, 1899, 2919, 3200
BstX2I RGATCY 2 cut(s) 2494, 2851
BstXI CCANNNNNNTGG 1 cut(s) 1872
BstYI RGATCY 2 cut(s) 2494, 2851
BstZ17I GTATAC 1 cut(s) 1832
Bsu36I CCTNAGG 1 cut(s) 2287
BsuRI GGCC 5 cut(s) 117, 1610, 1707, 2573, 2727
BtgI CCRYGG 1 cut(s) 1634
BtsCI GGATG 9 cut(s) 35, 370, 1155, 1441, 1445, 2218, 2278, 2559, 2831
BtsI GCAGTG 1 cut(s) 2334
BtsIMutI CAGTG 7 cut(s) 686, 979, 990, 1164, 2334, 2841, 3101
BveI ACCTGC 3 cut(s) 719, 832, 3230
Cac8I GCNNGC 6 cut(s) 339, 519, 1608, 2675, 3296, 3360
CfoI GCGC 5 cut(s) 375, 541, 2082, 3398, 3408
Cfr10I RCCGGY 1 cut(s) 2727
Cfr13I GGNCC 7 cut(s) 988, 1027, 1085, 1592, 1609, 1706, 2571
Cfr9I CCCGGG 1 cut(s) 1613
Csp6I GTAC 8 cut(s) 931, 1192, 1349, 1486, 1493, 1511, 2319, 2939
CspCI CAANNNNNGTGG 4 cut(s) 662, 697, 2451, 2486
CviQI GTAC 8 cut(s) 931, 1192, 1349, 1486, 1493, 1511, 2319, 2939
DraI TTTAAA 1 cut(s) 1284
DriI GACNNNNNGTC 1 cut(s) 3344
EaeI YGGCCR 1 cut(s) 2725
Eam1104I CTCTTC 3 cut(s) 214, 1933, 2121
Eam1105I GACNNNNNGTC 1 cut(s) 3344
EarI CTCTTC 3 cut(s) 214, 1933, 2121
Eco105I TACGTA 1 cut(s) 2897
Eco130I CCWWGG 2 cut(s) 1030, 1091
Eco31I GGTCTC 1 cut(s) 2113
Eco32I GATATC 1 cut(s) 3389
Eco47I GGWCC 4 cut(s) 988, 1027, 1085, 1592
Eco47III AGCGCT 1 cut(s) 2081
Eco57I CTGAAG 2 cut(s) 987, 1121
Eco81I CCTNAGG 1 cut(s) 2287
Eco88I CYCGRG 1 cut(s) 1613
EcoO109I RGGNCCY 3 cut(s) 1027, 1609, 1706
EcoRI GAATTC 2 cut(s) 307, 3418
EcoRII CCWGG 2 cut(s) 112, 264
EcoRV GATATC 1 cut(s) 3389
EcoT14I CCWWGG 2 cut(s) 1030, 1091
EcoT22I ATGCAT 4 cut(s) 37, 523, 2679, 3296
ErhI CCWWGG 2 cut(s) 1030, 1091
FalI AAGNNNNNCTT 4 cut(s) 1258, 1290, 1695, 1727
FaqI GGGAC 2 cut(s) 1040, 1913
FauNDI CATATG 1 cut(s) 1687
FbaI TGATCA 2 cut(s) 667, 1627
FblI GTMKAC 2 cut(s) 1831, 2311
FokI GGATG 9 cut(s) 22, 377, 1162, 1428, 1432, 2225, 2285, 2566, 2818
FspI TGCGCA 2 cut(s) 374, 3407
GlaI GCGC 5 cut(s) 374, 540, 2081, 3397, 3407
GsaI CCCAGC 3 cut(s) 1910, 2074, 2374
GsuI CTGGAG 4 cut(s) 96, 1334, 1650, 2319
HaeII RGCGCY 2 cut(s) 2083, 3399
HaeIII GGCC 5 cut(s) 117, 1610, 1707, 2573, 2727
HapII CCGG 6 cut(s) 101, 991, 1556, 1614, 1897, 2728
HhaI GCGC 5 cut(s) 375, 541, 2082, 3398, 3408
Hin6I GCGC 5 cut(s) 373, 539, 2080, 3396, 3406
HinP1I GCGC 5 cut(s) 373, 539, 2080, 3396, 3406
HincII GTYRAC 4 cut(s) 262, 1422, 2173, 2463
HindII GTYRAC 4 cut(s) 262, 1422, 2173, 2463
HindIII AAGCTT 2 cut(s) 1272, 3128
HpaII CCGG 6 cut(s) 101, 991, 1556, 1614, 1897, 2728
HphI GGTGA 8 cut(s) 905, 1310, 1622, 1685, 1805, 2114, 2900, 2938
HpyAV CCTTC 7 cut(s) 219, 500, 612, 1139, 1697, 2263, 2831
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4IV ACGT 2 cut(s) 1279, 2896
HpyF10VI GCNNNNNNNGC 7 cut(s) 347, 418, 1724, 1775, 2807, 2810, 3341
HpySE526I ACGT 2 cut(s) 1279, 2896
HspAI GCGC 5 cut(s) 373, 539, 2080, 3396, 3406
Kpn2I TCCGGA 1 cut(s) 100
KpnI GGTACC 1 cut(s) 1489
Ksp22I TGATCA 2 cut(s) 667, 1627
LguI GCTCTTC 1 cut(s) 2121
LmnI GCTCC 6 cut(s) 128, 211, 334, 355, 1371, 2219
LweI GCATC 5 cut(s) 22, 44, 1537, 1963, 2314
MaeII ACGT 2 cut(s) 1279, 2896
MaeIII GTNAC 9 cut(s) 923, 1544, 1657, 2248, 2447, 2581, 2730, 2888, 3152
MfeI CAATTG 1 cut(s) 1101
MflI RGATCY 2 cut(s) 2494, 2851
MhlI GDGCHC 4 cut(s) 352, 1441, 2336, 2619
MlyI GAGTC 4 cut(s) 1364, 1486, 2291, 3149
MmeI TCCRAC 4 cut(s) 360, 1433, 2113, 2618
Mph1103I ATGCAT 4 cut(s) 37, 523, 2679, 3296
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 3 cut(s) 1004, 1340, 1728
MslI CAYNNNNRTG 2 cut(s) 2053, 2606
MspA1I CMGCKG 1 cut(s) 3071
MspI CCGG 6 cut(s) 101, 991, 1556, 1614, 1897, 2728
MspR9I CCNGG 5 cut(s) 114, 266, 1614, 1615, 1898
MunI CAATTG 1 cut(s) 1101
Mva1269I GAATGC 5 cut(s) 429, 1585, 2478, 2509, 3209
MvaI CCWGG 2 cut(s) 114, 266
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 7 cut(s) 347, 418, 1724, 1775, 2807, 2810, 3341
NciI CCSGG 3 cut(s) 1614, 1615, 1898
NdeI CATATG 1 cut(s) 1687
NlaIV GGNNCC 9 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221, 2572
NmeAIII GCCGAG 1 cut(s) 2305
NmuCI GTSAC 4 cut(s) 1657, 2447, 2888, 3152
NsbI TGCGCA 2 cut(s) 374, 3407
NsiI ATGCAT 4 cut(s) 37, 523, 2679, 3296
NspI RCATGY 3 cut(s) 521, 2677, 3298
PaeI GCATGC 3 cut(s) 521, 2677, 3298
PciSI GCTCTTC 1 cut(s) 2121
PcsI WCGNNNNNNNCGW 1 cut(s) 1738
PctI GAATGC 5 cut(s) 429, 1585, 2478, 2509, 3209
PdmI GAANNNNTTC 3 cut(s) 1004, 1340, 1728
PfeI GAWTC 7 cut(s) 320, 860, 1536, 2033, 2042, 2356, 2518
PleI GAGTC 4 cut(s) 1363, 1485, 2290, 3149
PpsI GAGTC 4 cut(s) 1363, 1485, 2290, 3149
Ppu21I YACGTR 1 cut(s) 2897
PpuMI RGGWCCY 1 cut(s) 1027
Psp5II RGGWCCY 1 cut(s) 1027
Psp6I CCWGG 2 cut(s) 112, 264
PspFI CCCAGC 3 cut(s) 1906, 2070, 2370
PspGI CCWGG 2 cut(s) 112, 264
PspN4I GGNNCC 9 cut(s) 1028, 1036, 1188, 1373, 1438, 1487, 1611, 2221, 2572
PspPI GGNCC 7 cut(s) 988, 1027, 1085, 1592, 1609, 1706, 2571
PspPPI RGGWCCY 1 cut(s) 1027
PsuI RGATCY 2 cut(s) 2494, 2851
RsaI GTAC 8 cut(s) 932, 1193, 1350, 1487, 1494, 1512, 2320, 2940
RsaNI GTAC 8 cut(s) 931, 1192, 1349, 1486, 1493, 1511, 2319, 2939
RseI CAYNNNNRTG 2 cut(s) 2053, 2606
SapI GCTCTTC 1 cut(s) 2121
Sau96I GGNCC 7 cut(s) 988, 1027, 1085, 1592, 1609, 1706, 2571
ScaI AGTACT 2 cut(s) 1512, 2320
SchI GAGTC 4 cut(s) 1364, 1486, 2291, 3149
ScrFI CCNGG 5 cut(s) 114, 266, 1614, 1615, 1898
SduI GDGCHC 4 cut(s) 352, 1441, 2336, 2619
SfaNI GCATC 5 cut(s) 22, 44, 1537, 1963, 2314
SfcI CTRYAG 2 cut(s) 1040, 1384
SinI GGWCC 4 cut(s) 988, 1027, 1085, 1592
SmaI CCCGGG 1 cut(s) 1615
SmiMI CAYNNNNRTG 2 cut(s) 2053, 2606
SmlI CTYRAG 7 cut(s) 41, 287, 1238, 1289, 1698, 2719, 3271
SmoI CTYRAG 7 cut(s) 41, 287, 1238, 1289, 1698, 2719, 3271
SnaBI TACGTA 1 cut(s) 2897
SpeI ACTAGT 2 cut(s) 1247, 1654
SphI GCATGC 3 cut(s) 521, 2677, 3298
SsiI CCGC 4 cut(s) 545, 570, 2671, 3071
StyD4I CCNGG 5 cut(s) 112, 264, 1612, 1613, 1896
StyI CCWWGG 2 cut(s) 1030, 1091
TaaI ACNGT 1 cut(s) 595
TaiI ACGT 2 cut(s) 1282, 2899
TaqI TCGA 9 cut(s) 149, 434, 653, 1255, 1621, 1741, 2040, 3076, 3244
TaqII GACCGA 1 cut(s) 2952
TatI WGTACW 5 cut(s) 1348, 1492, 1510, 2318, 2938
TauI GCSGC 1 cut(s) 3074
TfiI GAWTC 7 cut(s) 320, 860, 1536, 2033, 2042, 2356, 2518
TscAI CASTG 7 cut(s) 686, 979, 990, 1171, 2341, 2848, 3108
TseFI GTSAC 4 cut(s) 1657, 2447, 2888, 3152
Tsp45I GTSAC 4 cut(s) 1657, 2447, 2888, 3152
TspGWI ACGGA 2 cut(s) 1179, 1584
TspMI CCCGGG 1 cut(s) 1613
TspRI CASTG 7 cut(s) 686, 979, 990, 1171, 2341, 2848, 3108
VneI GTGCAC 1 cut(s) 2615
VpaK11BI GGWCC 4 cut(s) 988, 1027, 1085, 1592
XapI RAATTY 7 cut(s) 48, 71, 307, 584, 1560, 1578, 3418
XbaI TCTAGA 1 cut(s) 1399
XceI RCATGY 3 cut(s) 521, 2677, 3298
XcmI CCANNNNNNNNNTGG 3 cut(s) 125, 2096, 2464
XmaI CCCGGG 1 cut(s) 1613
XmaJI CCTAGG 1 cut(s) 1030
XmiI GTMKAC 2 cut(s) 1831, 2311
XmnI GAANNNNTTC 3 cut(s) 1004, 1340, 1728
ZrmI AGTACT 2 cut(s) 1512, 2320
Zsp2I ATGCAT 4 cut(s) 37, 523, 2679, 3296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.