Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010683

Physical Location & Seq

Genomic Coordinates Forward (+)

38549934 .. 38552583

Gene Structure 2650 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1255000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 666 bp

ATGGGGCAGAAGGCGCTGATCTACGCGTTCGTGGCGCGCGGCACTGTGGTTCTCGCCGAGTACACGGAATTCAGCGGCAATTTCAACTCCATTGCGTTTCAGTGCCTCCAGAAGCTTCCTGCTACCAACAACAAGTTCACCTACAACTGCGATGGCCACACCTTCAATTACCTCGTCGACAATGGCTACACATACTGTGTGGTTGCAGATGAATCGAGTGGAAGACAAGTACCTATTGCTTTTCTGGAGCGTGTCAAGGATGACTTTGTCGCGAAATATGGTGCTGGAAAGGCTGCTACAGCTGCTGCCAACAGCCTTAACAAGGAATTTGGGTCAAAATTGAAGGAACATATGCAATACTGTGTTGATCATCCTGAAGAAATAAGCAAGCTTGCAAAGGTGAAGGCCCAGGTTTCAGAAGTTAAAGGCGTTATGATGGAGAATATTGAAAAGGTTTTGGATAGGGGGGAAAAGATAGAGCTTTTAGTCGACAAGACTGAGAACCTTCATCAACAGGCACAGGATTTTCGGACTGTGGGGACTAAAATGCGGAGGAAGATGTGGCTGCAGAACATGAAGGTGAAGCTGATTGTTTTAGGAATCCTGATCGCCTTAATCCTTATCATAATACTCTCCATTTGCCATGGTTTCAACTGCGGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0097708 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

222

Amino Acids

24.77

Weight (kDa)

8.8

Isoelectric Point (pI)

26.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	34 - 111	2.8e-23	Regulated-SNARE-like domain
Synaptobrevin	PF00957	130 - 210	2.6e-29	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04750 AT1G04760 AT1G04760 AT2G32670 AT2G33120 AT2G33120
fragaria_vesca	FvH4_3g12050
malus_domestica	MD05G1255000.v1.1 MD10G1235100.v1.1
prunus_persica	Prupe.4G108800_v2.0.a1
pyrus_communis	pycom05g23320 pycom10g19680
rosa_chinensis	RchiOBHm_Chr5g0019761
rosa_laevigata	RLG00000032466
rosa_multiflora	Rmu_ssc0000062.1_g000027
rosa_roxburghii	Rroxscaffold_1G00058370
rosa_rugosa	Rorug05G0053600
rosa_samantha	Rh5AG144200 Rh5BG142900
rosa_wichuraiana	Rw5G012750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	177, 489
AccII	CGCG	4 cut(s)	26, 37, 39, 272
AciI	CCGC	4 cut(s)	39, 75, 550, 657
AcoI	YGGCCR	1 cut(s)	154
AcsI	RAATTY	2 cut(s)	68, 326
AcuI	CTGAAG	1 cut(s)	396
AfaI	GTAC	2 cut(s)	62, 231
AfiI	CCNNNNNNNGG	1 cut(s)	322
AflIII	ACRYGT	1 cut(s)	24
AgsI	TTSAA	5 cut(s)	85, 166, 343, 449, 652
AjnI	CCWGG	1 cut(s)	408
AjuI	GAANNNNNNNTTGG	2 cut(s)	119, 151
AluBI	AGCT	5 cut(s)	115, 302, 391, 481, 586
AluI	AGCT	5 cut(s)	115, 302, 391, 481, 586
AlwNI	CAGNNNCTG	1 cut(s)	305
AoxI	GGCC	2 cut(s)	154, 405
ApeKI	GCWGC	4 cut(s)	293, 302, 305, 565
ApoI	RAATTY	2 cut(s)	68, 326
AspLEI	GCGC	3 cut(s)	16, 37, 39
AspS9I	GGNCC	1 cut(s)	406
AsuHPI	GGTGA	3 cut(s)	130, 412, 592
BalI	TGGCCA	1 cut(s)	156
BbsI	GAAGAC	1 cut(s)	229
BbvI	GCAGC	4 cut(s)	280, 289, 292, 552
BccI	CCATC	2 cut(s)	146, 430
BcgI	CGANNNNNNTGC	2 cut(s)	195, 229
BciT130I	CCWGG	1 cut(s)	410
BclI	TGATCA	1 cut(s)	367
BfmI	CTRYAG	2 cut(s)	297, 566
BfoI	RGCGCY	1 cut(s)	17
BisI	GCNGC	6 cut(s)	40, 76, 294, 303, 306, 566
BlsI	GCNGC	6 cut(s)	41, 77, 295, 304, 307, 567
Bme1390I	CCNGG	1 cut(s)	410
BmgT120I	GGNCC	1 cut(s)	406
BmrFI	CCNGG	1 cut(s)	410
BpiI	GAAGAC	1 cut(s)	229
BpmI	CTGGAG	2 cut(s)	92, 266
BsaJI	CCNNGG	2 cut(s)	408, 643
Bsc4I	CCNNNNNNNGG	1 cut(s)	322
Bse3DI	GCAATG	1 cut(s)	90
BseBI	CCWGG	1 cut(s)	410
BseDI	CCNNGG	2 cut(s)	408, 643
BseGI	GGATG	2 cut(s)	265, 370
BseLI	CCNNNNNNNGG	1 cut(s)	322
BseMI	GCAATG	1 cut(s)	90
BseMII	CTCAG	1 cut(s)	489
BsePI	GCGCGC	1 cut(s)	35
BseXI	GCAGC	4 cut(s)	280, 289, 292, 552
Bsh1236I	CGCG	4 cut(s)	26, 37, 39, 272
BshFI	GGCC	2 cut(s)	156, 407
BslFI	GGGAC	1 cut(s)	553
BslI	CCNNNNNNNGG	1 cut(s)	322
BsmFI	GGGAC	1 cut(s)	553
BsnI	GGCC	2 cut(s)	156, 407
Bsp143I	GATC	3 cut(s)	18, 367, 606
Bsp19I	CCATGG	1 cut(s)	643
Bsp68I	TCGCGA	1 cut(s)	272
BspACI	CCGC	4 cut(s)	39, 75, 550, 657
BspANI	GGCC	2 cut(s)	156, 407
BspCNI	CTCAG	1 cut(s)	490
BspFNI	CGCG	4 cut(s)	26, 37, 39, 272
BspMAI	CTGCAG	1 cut(s)	570
BsrDI	GCAATG	1 cut(s)	90
BssECI	CCNNGG	2 cut(s)	408, 643
BssHII	GCGCGC	1 cut(s)	35
BssMI	GATC	3 cut(s)	18, 367, 606
BssT1I	CCWWGG	1 cut(s)	643
Bst2UI	CCWGG	1 cut(s)	410
Bst4CI	ACNGT	4 cut(s)	46, 197, 362, 535
BstC8I	GCNNGC	3 cut(s)	37, 389, 393
BstDEI	CTNAG	1 cut(s)	498
BstDSI	CCRYGG	1 cut(s)	643
BstENI	CCTNNNNNAGG	1 cut(s)	320
BstF5I	GGATG	2 cut(s)	265, 370
BstFNI	CGCG	4 cut(s)	26, 37, 39, 272
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	3 cut(s)	16, 37, 39
BstKTI	GATC	3 cut(s)	21, 370, 609
BstMBI	GATC	3 cut(s)	18, 367, 606
BstMWI	GCNNNNNNNGC	5 cut(s)	13, 32, 290, 299, 302
BstNI	CCWGG	1 cut(s)	410
BstSCI	CCNGG	1 cut(s)	408
BstSFI	CTRYAG	2 cut(s)	297, 566
BstUI	CGCG	4 cut(s)	26, 37, 39, 272
BstV1I	GCAGC	4 cut(s)	280, 289, 292, 552
BstV2I	GAAGAC	1 cut(s)	229
BsuRI	GGCC	2 cut(s)	156, 407
BtgI	CCRYGG	1 cut(s)	643
BtgZI	GCGATG	1 cut(s)	165
BtsCI	GGATG	2 cut(s)	265, 370
BtsIMutI	CAGTG	2 cut(s)	42, 107
BtuMI	TCGCGA	1 cut(s)	272
Cac8I	GCNNGC	3 cut(s)	37, 389, 393
CaiI	CAGNNNCTG	1 cut(s)	305
CfoI	GCGC	3 cut(s)	16, 37, 39
Cfr13I	GGNCC	1 cut(s)	406
Csp6I	GTAC	2 cut(s)	61, 230
CviAII	CATG	2 cut(s)	574, 644
CviQI	GTAC	2 cut(s)	61, 230
DdeI	CTNAG	1 cut(s)	498
DpnI	GATC	3 cut(s)	20, 369, 608
DpnII	GATC	3 cut(s)	18, 367, 606
EaeI	YGGCCR	1 cut(s)	154
Eco130I	CCWWGG	1 cut(s)	643
Eco57I	CTGAAG	1 cut(s)	396
EcoNI	CCTNNNNNAGG	1 cut(s)	320
EcoRI	GAATTC	1 cut(s)	68
EcoRII	CCWGG	1 cut(s)	408
EcoT14I	CCWWGG	1 cut(s)	643
ErhI	CCWWGG	1 cut(s)	643
FaeI	CATG	2 cut(s)	577, 647
FaiI	YATR	8 cut(s)	193, 279, 351, 353, 434, 575, 626, 645
FalI	AAGNNNNNCTT	2 cut(s)	248, 280
FaqI	GGGAC	1 cut(s)	553
FatI	CATG	2 cut(s)	573, 643
FauNDI	CATATG	1 cut(s)	351
FbaI	TGATCA	1 cut(s)	367
FblI	GTMKAC	2 cut(s)	177, 489
Fnu4HI	GCNGC	6 cut(s)	40, 76, 294, 303, 306, 566
FokI	GGATG	2 cut(s)	272, 357
Fsp4HI	GCNGC	6 cut(s)	40, 76, 294, 303, 306, 566
GlaI	GCGC	3 cut(s)	15, 36, 38
GluI	GCNGC	6 cut(s)	40, 76, 294, 303, 306, 566
GsuI	CTGGAG	2 cut(s)	92, 266
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	2 cut(s)	156, 407
HhaI	GCGC	3 cut(s)	16, 37, 39
Hin1II	CATG	2 cut(s)	577, 647
Hin6I	GCGC	3 cut(s)	14, 35, 37
HinP1I	GCGC	3 cut(s)	14, 35, 37
HincII	GTYRAC	2 cut(s)	178, 490
HindII	GTYRAC	2 cut(s)	178, 490
HindIII	AAGCTT	2 cut(s)	113, 389
HinfI	GANTC	2 cut(s)	212, 600
HphI	GGTGA	3 cut(s)	130, 412, 592
Hpy166II	GTNNAC	4 cut(s)	63, 138, 178, 490
Hpy188I	TCNGA	2 cut(s)	418, 531
Hpy188III	TCNNGA	5 cut(s)	109, 245, 271, 374, 604
Hpy8I	GTNNAC	4 cut(s)	63, 138, 178, 490
Hpy99I	CGWCG	1 cut(s)	179
HpyAV	CCTTC	6 cut(s)	4, 172, 337, 397, 515, 571
HpyCH4III	ACNGT	4 cut(s)	46, 197, 362, 535
HpyCH4V	TGCA	4 cut(s)	206, 355, 395, 568
HpyF10VI	GCNNNNNNNGC	5 cut(s)	13, 32, 290, 299, 302
HpyF3I	CTNAG	1 cut(s)	498
Hsp92II	CATG	2 cut(s)	577, 647
HspAI	GCGC	3 cut(s)	14, 35, 37
Ksp22I	TGATCA	1 cut(s)	367
Kzo9I	GATC	3 cut(s)	18, 367, 606
LmnI	GCTCC	1 cut(s)	247
Lsp1109I	GCAGC	4 cut(s)	280, 289, 292, 552
MalI	GATC	3 cut(s)	20, 369, 608
MboI	GATC	3 cut(s)	18, 367, 606
MboII	GAAGA	3 cut(s)	234, 389, 568
MlsI	TGGCCA	1 cut(s)	156
MluCI	AATT	5 cut(s)	68, 79, 166, 326, 338
MluI	ACGCGT	1 cut(s)	24
MluNI	TGGCCA	1 cut(s)	156
MnlI	CCTC	3 cut(s)	116, 182, 546
Mox20I	TGGCCA	1 cut(s)	156
MscI	TGGCCA	1 cut(s)	156
MseI	TTAA	3 cut(s)	318, 423, 614
MslI	CAYNNNNRTG	1 cut(s)	578
Msp20I	TGGCCA	1 cut(s)	156
MspA1I	CMGCKG	2 cut(s)	75, 302
MspR9I	CCNGG	1 cut(s)	410
MvaI	CCWGG	1 cut(s)	410
MvnI	CGCG	4 cut(s)	26, 37, 39, 272
MwoI	GCNNNNNNNGC	5 cut(s)	13, 32, 290, 299, 302
NcoI	CCATGG	1 cut(s)	643
NdeI	CATATG	1 cut(s)	351
NdeII	GATC	3 cut(s)	18, 367, 606
NlaIII	CATG	2 cut(s)	577, 647
NmeAIII	GCCGAG	1 cut(s)	82
NruI	TCGCGA	1 cut(s)	272
PauI	GCGCGC	1 cut(s)	35
PfeI	GAWTC	2 cut(s)	212, 600
PflFI	GACNNNGTC	1 cut(s)	266
PkrI	GCNGC	6 cut(s)	41, 77, 295, 304, 307, 567
Psp6I	CCWGG	1 cut(s)	408
PspGI	CCWGG	1 cut(s)	408
PspPI	GGNCC	1 cut(s)	406
PstI	CTGCAG	1 cut(s)	570
PstNI	CAGNNNCTG	1 cut(s)	305
PsyI	GACNNNGTC	1 cut(s)	266
PteI	GCGCGC	1 cut(s)	35
PvuII	CAGCTG	1 cut(s)	302
RruI	TCGCGA	1 cut(s)	272
RsaI	GTAC	2 cut(s)	62, 231
RsaNI	GTAC	2 cut(s)	61, 230
RseI	CAYNNNNRTG	1 cut(s)	578
SalI	GTCGAC	2 cut(s)	176, 488
SaqAI	TTAA	3 cut(s)	318, 423, 614
SatI	GCNGC	6 cut(s)	40, 76, 294, 303, 306, 566
Sau3AI	GATC	3 cut(s)	18, 367, 606
Sau96I	GGNCC	1 cut(s)	406
ScrFI	CCNGG	1 cut(s)	410
SfcI	CTRYAG	2 cut(s)	297, 566
SmiMI	CAYNNNNRTG	1 cut(s)	578
Sse9I	AATT	5 cut(s)	68, 79, 166, 326, 338
SsiI	CCGC	4 cut(s)	39, 75, 550, 657
SspI	AATATT	1 cut(s)	445
StyD4I	CCNGG	1 cut(s)	408
StyI	CCWWGG	1 cut(s)	643
TaaI	ACNGT	4 cut(s)	46, 197, 362, 535
TaqI	TCGA	3 cut(s)	177, 215, 489
TasI	AATT	5 cut(s)	68, 79, 166, 326, 338
TatI	WGTACW	1 cut(s)	60
TauI	GCSGC	2 cut(s)	42, 78
TfiI	GAWTC	2 cut(s)	212, 600
Tru1I	TTAA	3 cut(s)	318, 423, 614
Tru9I	TTAA	3 cut(s)	318, 423, 614
TscAI	CASTG	2 cut(s)	49, 107
TseI	GCWGC	4 cut(s)	293, 302, 305, 565
TspDTI	ATGAA	3 cut(s)	225, 497, 590
TspGWI	ACGGA	1 cut(s)	80
TspRI	CASTG	2 cut(s)	49, 107
Tth111I	GACNNNGTC	1 cut(s)	266
XagI	CCTNNNNNAGG	1 cut(s)	320
XapI	RAATTY	2 cut(s)	68, 326
XmiI	GTMKAC	2 cut(s)	177, 489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.