MD05G1310700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012590
Physical Location & Seq
Forward (+)
44116324 .. 44118313
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1310700.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGTTGCAGAGGATCTTGAAATGCACCATGGATGGCCCCTAGGGCTTGAGATCATGAACATGAGACTTAGAGTGGTGGGGAGCTTACCAGCTGCAGTTGTAGAACACCAGTCCTCGCACCTTCGCTCCGCCAGTTTCACCTCCTTTTCGTCCTCCAACCTTGACACCGAGTCCTCAGCATCCTTCTTCCAAGACCACAGCATGTCACTAGGCCGACTAATTGGGATTCGAACGGGTGATAGAGGACGGTTGTACTTCCCCAATTCCATCCGCTTCGAAGAGCATGACAGGATTTCGATCAAGGGTTCGCAGTCTGAGGTCTCCGCGAGACGTCGAGTAGACATGTCTCGGCGTATCTGCATCCCGCTGCTGCTTGGAGCTCTAGTAAAGATCAGCAGGAGTAAGAGCAAGAGCAAGCCTAAGAAGGCAATGTTCGATGCCAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.69

Weight (kDa)

10.38

Isoelectric Point (pI)

58.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 339
AccII CGCG 1 cut(s) 326
AciI CCGC 4 cut(s) 129, 271, 324, 365
AclWI GGATC 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 331
AfaI GTAC 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 1 cut(s) 20
AhdI GACNNNNNGTC 1 cut(s) 169
AluBI AGCT 3 cut(s) 84, 92, 380
AluI AGCT 3 cut(s) 84, 92, 380
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 4 cut(s) 58, 322, 325, 351
AlwI GGATC 1 cut(s) 21
AoxI GGCC 2 cut(s) 35, 211
ApeKI GCWGC 3 cut(s) 92, 367, 370
AspA2I CCTAGG 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 36
AsuHPI GGTGA 2 cut(s) 130, 248
AsuII TTCGAA 2 cut(s) 229, 276
AvrII CCTAGG 1 cut(s) 40
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvCI CCTCAGC 1 cut(s) 175
BbvI GCAGC 3 cut(s) 79, 354, 357
BccI CCATC 2 cut(s) 27, 275
BcoDI GTCTC 4 cut(s) 58, 322, 325, 351
BfaI CTAG 3 cut(s) 41, 209, 383
BfmI CTRYAG 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 43
BisI GCNGC 3 cut(s) 93, 368, 371
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 3 cut(s) 94, 369, 372
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 1 cut(s) 38
BmsI GCATC 3 cut(s) 188, 369, 427
Bpu10I CCTNAGC 1 cut(s) 175
Bpu14I TTCGAA 2 cut(s) 229, 276
BpuEI CTTGAG 1 cut(s) 68
BsaBI GATNNNNATC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 331
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 2 cut(s) 28, 40
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 369, 399
Bse1I ACTGG 2 cut(s) 109, 132
Bse3DI GCAATG 1 cut(s) 435
Bse8I GATNNNNATC 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 28, 40
BseGI GGATG 4 cut(s) 38, 179, 267, 360
BseJI GATNNNNATC 1 cut(s) 296
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 2 cut(s) 189, 306
BseNI ACTGG 2 cut(s) 109, 132
BseXI GCAGC 3 cut(s) 79, 354, 357
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 2 cut(s) 37, 213
BsiHKAI GWGCWC 1 cut(s) 382
BsmAI GTCTC 4 cut(s) 58, 322, 325, 351
BsmBI CGTCTC 1 cut(s) 322
BsnI GGCC 2 cut(s) 37, 213
Bso31I GGTCTC 1 cut(s) 325
Bsp119I TTCGAA 2 cut(s) 229, 276
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 4 cut(s) 13, 51, 297, 390
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 4 cut(s) 129, 271, 324, 365
BspANI GGCC 2 cut(s) 37, 213
BspCNI CTCAG 2 cut(s) 188, 307
BspFNI CGCG 1 cut(s) 326
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 1 cut(s) 21
BspQI GCTCTTC 1 cut(s) 273
BspT104I TTCGAA 2 cut(s) 229, 276
BspTNI GGTCTC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 435
BsrI ACTGG 2 cut(s) 109, 132
BssECI CCNNGG 2 cut(s) 28, 40
BssMI GATC 4 cut(s) 13, 51, 297, 390
BssNI GRCGYC 1 cut(s) 331
BssT1I CCWWGG 2 cut(s) 28, 40
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 273
BstACI GRCGYC 1 cut(s) 331
BstBI TTCGAA 2 cut(s) 229, 276
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 4 cut(s) 68, 175, 315, 420
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 4 cut(s) 38, 179, 267, 360
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 4 cut(s) 16, 54, 300, 393
BstMAI GTCTC 4 cut(s) 58, 322, 325, 351
BstMBI GATC 4 cut(s) 13, 51, 297, 390
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNSI RCATGY 2 cut(s) 205, 346
BstSFI CTRYAG 1 cut(s) 93
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 3 cut(s) 79, 354, 357
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 2 cut(s) 37, 213
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 4 cut(s) 38, 179, 267, 360
Cac8I GCNNGC 1 cut(s) 416
CciI TCATGA 1 cut(s) 54
Cfr13I GGNCC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 253
CviAII CATG 6 cut(s) 29, 55, 61, 202, 284, 343
CviJI RGCY 8 cut(s) 37, 46, 84, 92, 213, 380, 418, 447
CviKI_1 RGCY 8 cut(s) 37, 46, 84, 92, 213, 380, 418, 447
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 4 cut(s) 68, 175, 315, 420
DpnI GATC 4 cut(s) 15, 53, 299, 392
DpnII GATC 4 cut(s) 13, 51, 297, 390
DriI GACNNNNNGTC 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 273
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 28, 40
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 380
EcoT14I CCWWGG 2 cut(s) 28, 40
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 28, 40
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 6 cut(s) 32, 58, 64, 205, 287, 346
FaiI YATR 6 cut(s) 30, 56, 62, 203, 285, 344
FatI CATG 6 cut(s) 28, 54, 60, 201, 283, 342
FauI CCCGC 1 cut(s) 372
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 3 cut(s) 93, 368, 371
FokI GGATG 4 cut(s) 45, 166, 254, 347
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 3 cut(s) 93, 368, 371
FspBI CTAG 3 cut(s) 41, 209, 383
GluI GCNGC 3 cut(s) 93, 368, 371
HaeIII GGCC 2 cut(s) 37, 213
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 6 cut(s) 32, 58, 64, 205, 287, 346
HinfI GANTC 2 cut(s) 170, 226
HphI GGTGA 2 cut(s) 130, 248
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 17, 55
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 3 cut(s) 131, 193, 418
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 1 cut(s) 331
HpyCH4V TGCA 4 cut(s) 8, 25, 95, 360
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 4 cut(s) 68, 175, 315, 420
HpySE526I ACGT 1 cut(s) 331
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 6 cut(s) 32, 58, 64, 205, 287, 346
Kzo9I GATC 4 cut(s) 13, 51, 297, 390
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 3 cut(s) 81, 131, 377
LpnPI CCDG 5 cut(s) 102, 122, 145, 274, 382
Lsp1109I GCAGC 3 cut(s) 79, 354, 357
LweI GCATC 3 cut(s) 188, 369, 427
MaeI CTAG 3 cut(s) 41, 209, 383
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 1 cut(s) 204
MalI GATC 4 cut(s) 15, 53, 299, 392
MboI GATC 4 cut(s) 13, 51, 297, 390
MboII GAAGA 2 cut(s) 178, 290
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 2 cut(s) 219, 262
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 8 cut(s) 4, 124, 151, 163, 184, 236, 310, 437
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 2 cut(s) 92, 367
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 43
NcoI CCATGG 1 cut(s) 28
NdeII GATC 4 cut(s) 13, 51, 297, 390
NlaIII CATG 6 cut(s) 32, 58, 64, 205, 287, 346
NlaIV GGNNCC 1 cut(s) 38
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 204
NspI RCATGY 2 cut(s) 205, 346
NspV TTCGAA 2 cut(s) 229, 276
PagI TCATGA 1 cut(s) 54
PciI ACATGT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 3 cut(s) 94, 369, 372
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PscI ACATGT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 382
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 1 cut(s) 36
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 273
SatI GCNGC 3 cut(s) 93, 368, 371
Sau3AI GATC 4 cut(s) 13, 51, 297, 390
Sau96I GGNCC 1 cut(s) 36
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 1 cut(s) 382
SetI ASST 8 cut(s) 86, 94, 123, 143, 162, 321, 334, 382
SfaNI GCATC 3 cut(s) 188, 369, 427
SfcI CTRYAG 1 cut(s) 93
SfuI TTCGAA 2 cut(s) 229, 276
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 2 cut(s) 219, 262
SsiI CCGC 4 cut(s) 129, 271, 324, 365
SspMI CTAG 3 cut(s) 41, 209, 383
SstI GAGCTC 1 cut(s) 382
StyI CCWWGG 2 cut(s) 28, 40
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 1 cut(s) 334
TaqI TCGA 5 cut(s) 229, 276, 296, 334, 435
TasI AATT 2 cut(s) 219, 262
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 226
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 3 cut(s) 92, 367, 370
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 1 cut(s) 71
XceI RCATGY 2 cut(s) 205, 346
XmaJI CCTAGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 3 cut(s) 41, 209, 383
ZraI GACGTC 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.