pycom05g28700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012590
Physical Location & Seq
Forward (+)
29325827 .. 29327082
1256 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28700.3

Sequence Viewer

Length: 444 bp
ATGGTTGCAGAGGATCTTGAAATGCACCATGGATGGCCCCTGGGGCTTGAGATCATGAACATGAGACTTAGAGTGGTGGAGAGCTTACCAGCTGCAGTTGTAGAACACCGGTCCTCGCACGTTCGCTCCACCAGTTTCACCTCCTTTTCGTCCTCCAACCTTGACACCGAGTCCTCAGCATCCTTCTTCCAAGACCACAGCATGTCACTAGGCCGACTAATTGGGATTCGAACGGGTGATAGAGGACGGTTGTACTTCCCCAATTCCATCCGCTTCGAAGAGCATGACAGGATTCCGATCAAGGGTTCGCAGTCTGAGGTCTCTAAGAGACGTCGAGTAGACATGTCTCGGCGTATCTGCATACCGCTGCTGCTTGGTGCTCTGGTAAAGATCAGCAGGAGCAAGAGCAAGCCTAAGAAGGCAATGTTTGATGCCAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.66

Weight (kDa)

10.38

Isoelectric Point (pI)

62.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 339
AciI CCGC 2 cut(s) 271, 365
AclWI GGATC 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 331
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 342
AgeI ACCGGT 1 cut(s) 108
AgsI TTSAA 1 cut(s) 20
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 2 cut(s) 84, 92
AluI AGCT 2 cut(s) 84, 92
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 4 cut(s) 58, 322, 325, 351
AlwI GGATC 1 cut(s) 21
AoxI GGCC 2 cut(s) 35, 211
ApeKI GCWGC 3 cut(s) 92, 367, 370
AsiGI ACCGGT 1 cut(s) 108
AspS9I GGNCC 2 cut(s) 36, 111
AsuHPI GGTGA 2 cut(s) 130, 248
AsuII TTCGAA 2 cut(s) 229, 276
AvaII GGWCC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 382
BbvCI CCTCAGC 1 cut(s) 175
BbvI GCAGC 3 cut(s) 79, 354, 357
BccI CCATC 2 cut(s) 27, 275
BciT130I CCWGG 1 cut(s) 41
BcoDI GTCTC 4 cut(s) 58, 322, 325, 351
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 43
BisI GCNGC 3 cut(s) 93, 368, 371
BlsI GCNGC 3 cut(s) 94, 369, 372
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 111
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 36, 111
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 2 cut(s) 188, 421
Bpu10I CCTNAGC 1 cut(s) 175
Bpu14I TTCGAA 2 cut(s) 229, 276
BpuEI CTTGAG 1 cut(s) 68
BsaBI GATNNNNATC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 331
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 28, 39, 40
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 3 cut(s) 28, 39, 40
BseGI GGATG 3 cut(s) 38, 179, 267
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 189, 306
BseNI ACTGG 1 cut(s) 132
BseXI GCAGC 3 cut(s) 79, 354, 357
BshFI GGCC 2 cut(s) 37, 213
BshTI ACCGGT 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 4 cut(s) 58, 322, 325, 351
BsmBI CGTCTC 1 cut(s) 322
BsnI GGCC 2 cut(s) 37, 213
Bso31I GGTCTC 1 cut(s) 325
Bsp119I TTCGAA 2 cut(s) 229, 276
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 4 cut(s) 13, 51, 297, 390
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 2 cut(s) 271, 365
BspANI GGCC 2 cut(s) 37, 213
BspCNI CTCAG 2 cut(s) 188, 307
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 1 cut(s) 21
BspQI GCTCTTC 1 cut(s) 273
BspT104I TTCGAA 2 cut(s) 229, 276
BspTNI GGTCTC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 108
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 28, 39, 40
BssMI GATC 4 cut(s) 13, 51, 297, 390
BssNI GRCGYC 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 273
BstACI GRCGYC 1 cut(s) 331
BstBI TTCGAA 2 cut(s) 229, 276
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 5 cut(s) 68, 175, 315, 324, 414
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 3 cut(s) 38, 179, 267
BstKTI GATC 4 cut(s) 16, 54, 300, 393
BstMAI GTCTC 4 cut(s) 58, 322, 325, 351
BstMBI GATC 4 cut(s) 13, 51, 297, 390
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 2 cut(s) 205, 346
BstSCI CCNGG 1 cut(s) 39
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 79, 354, 357
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 2 cut(s) 37, 213
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 3 cut(s) 38, 179, 267
Cac8I GCNNGC 1 cut(s) 410
CciI TCATGA 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 36, 111
Csp6I GTAC 1 cut(s) 253
CspAI ACCGGT 1 cut(s) 108
CviAII CATG 6 cut(s) 29, 55, 61, 202, 284, 343
CviJI RGCY 7 cut(s) 37, 46, 84, 92, 213, 412, 441
CviKI_1 RGCY 7 cut(s) 37, 46, 84, 92, 213, 412, 441
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 5 cut(s) 68, 175, 315, 324, 414
DpnI GATC 4 cut(s) 15, 53, 299, 392
DpnII GATC 4 cut(s) 13, 51, 297, 390
DriI GACNNNNNGTC 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 273
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 325
Eco47I GGWCC 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 6 cut(s) 32, 58, 64, 205, 287, 346
FaiI YATR 7 cut(s) 30, 56, 62, 203, 285, 344, 362
FatI CATG 6 cut(s) 28, 54, 60, 201, 283, 342
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 3 cut(s) 93, 368, 371
FokI GGATG 3 cut(s) 45, 166, 254
Fsp4HI GCNGC 3 cut(s) 93, 368, 371
FspBI CTAG 1 cut(s) 209
GluI GCNGC 3 cut(s) 93, 368, 371
HaeIII GGCC 2 cut(s) 37, 213
HapII CCGG 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 6 cut(s) 32, 58, 64, 205, 287, 346
HinfI GANTC 3 cut(s) 170, 226, 292
HpaII CCGG 1 cut(s) 109
HphI GGTGA 2 cut(s) 130, 248
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 297, 316
Hpy188III TCNNGA 2 cut(s) 17, 55
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 2 cut(s) 193, 412
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 120, 331
HpyCH4V TGCA 4 cut(s) 8, 25, 95, 360
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 5 cut(s) 68, 175, 315, 324, 414
HpySE526I ACGT 2 cut(s) 120, 331
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 6 cut(s) 32, 58, 64, 205, 287, 346
Kzo9I GATC 4 cut(s) 13, 51, 297, 390
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 131, 399
LpnPI CCDG 8 cut(s) 26, 53, 102, 122, 145, 274, 368, 382
Lsp1109I GCAGC 3 cut(s) 79, 354, 357
LweI GCATC 2 cut(s) 188, 421
MaeI CTAG 1 cut(s) 209
MaeII ACGT 2 cut(s) 120, 331
MaeIII GTNAC 1 cut(s) 204
MalI GATC 4 cut(s) 15, 53, 299, 392
MboI GATC 4 cut(s) 13, 51, 297, 390
MboII GAAGA 2 cut(s) 178, 290
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 2 cut(s) 219, 262
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 8 cut(s) 4, 124, 151, 163, 184, 236, 310, 431
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 2 cut(s) 92, 367
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 43
NcoI CCATGG 1 cut(s) 28
NdeII GATC 4 cut(s) 13, 51, 297, 390
NlaIII CATG 6 cut(s) 32, 58, 64, 205, 287, 346
NlaIV GGNNCC 1 cut(s) 38
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 204
NspI RCATGY 2 cut(s) 205, 346
NspV TTCGAA 2 cut(s) 229, 276
PagI TCATGA 1 cut(s) 54
PasI CCCWGGG 1 cut(s) 40
PciI ACATGT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 226, 292
PinAI ACCGGT 1 cut(s) 108
PkrI GCNGC 3 cut(s) 94, 369, 372
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PscI ACATGT 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 2 cut(s) 36, 111
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 273
SatI GCNGC 3 cut(s) 93, 368, 371
Sau3AI GATC 4 cut(s) 13, 51, 297, 390
Sau96I GGNCC 2 cut(s) 36, 111
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 382
SetI ASST 7 cut(s) 86, 94, 123, 143, 162, 321, 334
SfaNI GCATC 2 cut(s) 188, 421
SfcI CTRYAG 1 cut(s) 93
SfuI TTCGAA 2 cut(s) 229, 276
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 2 cut(s) 219, 262
SsiI CCGC 2 cut(s) 271, 365
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 2 cut(s) 123, 334
TaqI TCGA 3 cut(s) 229, 276, 334
TasI AATT 2 cut(s) 219, 262
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 226, 292
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 3 cut(s) 92, 367, 370
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 111
XceI RCATGY 2 cut(s) 205, 346
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 1 cut(s) 209
ZraI GACGTC 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.