MD10G1289800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012590
Physical Location & Seq
Forward (+)
37862077 .. 37864069
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1289800.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGTCACAGGCGTCGATCATGACATGCACCATGGATGGCCCCTAGGGCTTGAGATTATGAACGTGAGACTTAGAGTGGTGGAGAGCTTACCAGCTGCAGTTGTAGGGCGCCGGTCCTCGCACTTGCCCTCCGCCAGTTTCACCTCCTTCTCGTCCTCCAACCTTGACACCGAGTCCTCAGCATCCTTCTTCCAAGACCACAGCACGTCACTAGGCCGTCTGATTGGGCTTCGAACGGGTGATAGAGGACGTTTGTACTTACCAAATTCCATTCGCTTCGAAGAGCACGAGAGGATTTCGATTAGGGGTTCGCATTCTGAGGTGTCTACGAGACATCGAGTAGACATGTCTCGGCGTATCTGCATACCGTTGCTGCTTTGTGCTCTAGTAAAGATCAGCAGGAGTAAGAACAAGAGCAAGTCAAAGAAGGCAAAGTTTGATGGCAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.75

Weight (kDa)

10.6

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AccI GTMKAC 2 cut(s) 326, 342
AciI CCGC 1 cut(s) 132
AcsI RAATTY 1 cut(s) 265
AcyI GRCGYC 2 cut(s) 12, 109
AfaI GTAC 1 cut(s) 257
AflIII ACRYGT 1 cut(s) 345
AhdI GACNNNNNGTC 1 cut(s) 172
AjiI CACGTC 1 cut(s) 207
AluBI AGCT 2 cut(s) 87, 95
AluI AGCT 2 cut(s) 87, 95
Alw21I GWGCWC 2 cut(s) 288, 385
Alw26I GTCTC 3 cut(s) 61, 325, 354
AoxI GGCC 2 cut(s) 38, 214
ApeKI GCWGC 2 cut(s) 95, 373
ApoI RAATTY 1 cut(s) 265
AspA2I CCTAGG 1 cut(s) 43
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 39, 114
AsuHPI GGTGA 2 cut(s) 133, 251
AsuII TTCGAA 2 cut(s) 232, 279
AvaII GGWCC 1 cut(s) 114
AvrII CCTAGG 1 cut(s) 43
BanI GGYRCC 1 cut(s) 108
BauI CACGAG 1 cut(s) 287
Bbv12I GWGCWC 2 cut(s) 288, 385
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 82, 360
BccI CCATC 2 cut(s) 30, 434
BceAI ACGGC 1 cut(s) 201
BcoDI GTCTC 3 cut(s) 61, 325, 354
BfaI CTAG 3 cut(s) 44, 212, 386
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 112
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 2 cut(s) 96, 374
BlnI CCTAGG 1 cut(s) 43
BlsI GCNGC 2 cut(s) 97, 375
Bme18I GGWCC 1 cut(s) 114
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 39, 114
BmiI GGNNCC 2 cut(s) 41, 110
BmsI GCATC 1 cut(s) 191
Bpu10I CCTNAGC 1 cut(s) 178
Bpu14I TTCGAA 2 cut(s) 232, 279
BpuEI CTTGAG 1 cut(s) 71
BsaHI GRCGYC 2 cut(s) 12, 109
BsaJI CCNNGG 2 cut(s) 31, 43
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 31, 43
BseGI GGATG 2 cut(s) 41, 182
BseMII CTCAG 2 cut(s) 192, 309
BseNI ACTGG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 82, 360
BshFI GGCC 2 cut(s) 40, 216
BshNI GGYRCC 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 288, 385
BsiSI CCGG 1 cut(s) 112
BsmAI GTCTC 3 cut(s) 61, 325, 354
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 2 cut(s) 40, 216
Bsp119I TTCGAA 2 cut(s) 232, 279
Bsp1286I GDGCHC 2 cut(s) 288, 385
Bsp143I GATC 2 cut(s) 16, 393
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 1 cut(s) 132
BspANI GGCC 2 cut(s) 40, 216
BspCNI CTCAG 2 cut(s) 191, 310
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 41, 110
BspMAI CTGCAG 1 cut(s) 100
BspQI GCTCTTC 1 cut(s) 276
BspT104I TTCGAA 2 cut(s) 232, 279
BspT107I GGYRCC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 135
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 31, 43
BssMI GATC 2 cut(s) 16, 393
BssNI GRCGYC 2 cut(s) 12, 109
BssSI CACGAG 1 cut(s) 287
BssT1I CCWWGG 2 cut(s) 31, 43
Bst2BI CACGAG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 369
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 2 cut(s) 12, 109
BstBI TTCGAA 2 cut(s) 232, 279
BstDEI CTNAG 3 cut(s) 71, 178, 318
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 41, 182
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 2 cut(s) 19, 396
BstMAI GTCTC 3 cut(s) 61, 325, 354
BstMBI GATC 2 cut(s) 16, 393
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNSI RCATGY 2 cut(s) 28, 349
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 82, 360
BsuRI GGCC 2 cut(s) 40, 216
BtgI CCRYGG 1 cut(s) 31
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 41, 182
CciI TCATGA 1 cut(s) 19
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 2 cut(s) 39, 114
Csp6I GTAC 1 cut(s) 256
CviAII CATG 4 cut(s) 20, 25, 32, 346
CviJI RGCY 7 cut(s) 40, 49, 87, 95, 216, 229, 450
CviKI_1 RGCY 7 cut(s) 40, 49, 87, 95, 216, 229, 450
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 3 cut(s) 71, 178, 318
DinI GGCGCC 1 cut(s) 110
DpnI GATC 2 cut(s) 18, 395
DpnII GATC 2 cut(s) 16, 393
DriI GACNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 121
Eco130I CCWWGG 2 cut(s) 31, 43
Eco47I GGWCC 1 cut(s) 114
EcoT14I CCWWGG 2 cut(s) 31, 43
EgeI GGCGCC 1 cut(s) 110
EheI GGCGCC 1 cut(s) 110
ErhI CCWWGG 2 cut(s) 31, 43
FaeI CATG 4 cut(s) 23, 28, 35, 349
FaiI YATR 6 cut(s) 21, 26, 33, 59, 347, 365
FatI CATG 4 cut(s) 19, 24, 31, 345
FblI GTMKAC 2 cut(s) 326, 342
Fnu4HI GCNGC 2 cut(s) 96, 374
FokI GGATG 2 cut(s) 48, 169
Fsp4HI GCNGC 2 cut(s) 96, 374
FspBI CTAG 3 cut(s) 44, 212, 386
GlaI GCGC 1 cut(s) 110
GluI GCNGC 2 cut(s) 96, 374
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 2 cut(s) 40, 216
HapII CCGG 1 cut(s) 112
HhaI GCGC 1 cut(s) 111
Hin1I GRCGYC 2 cut(s) 12, 109
Hin1II CATG 4 cut(s) 23, 28, 35, 349
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 112
HphI GGTGA 2 cut(s) 133, 251
Hpy166II GTNNAC 2 cut(s) 327, 343
Hpy188I TCNGA 2 cut(s) 222, 319
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 2 cut(s) 327, 343
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 157, 196, 421
HpyCH4III ACNGT 1 cut(s) 369
HpyCH4IV ACGT 3 cut(s) 63, 206, 250
HpyCH4V TGCA 3 cut(s) 28, 98, 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 3 cut(s) 71, 178, 318
HpySE526I ACGT 3 cut(s) 63, 206, 250
Hsp92I GRCGYC 2 cut(s) 12, 109
Hsp92II CATG 4 cut(s) 23, 28, 35, 349
HspAI GCGC 1 cut(s) 109
KasI GGCGCC 1 cut(s) 108
Kzo9I GATC 2 cut(s) 16, 393
LguI GCTCTTC 1 cut(s) 276
LpnPI CCDG 4 cut(s) 105, 125, 148, 385
Lsp1109I GCAGC 2 cut(s) 82, 360
LweI GCATC 1 cut(s) 191
MaeI CTAG 3 cut(s) 44, 212, 386
MaeII ACGT 3 cut(s) 63, 206, 250
MaeIII GTNAC 2 cut(s) 4, 207
MalI GATC 2 cut(s) 18, 395
MboI GATC 2 cut(s) 16, 393
MboII GAAGA 2 cut(s) 181, 293
MhlI GDGCHC 2 cut(s) 288, 385
MluCI AATT 1 cut(s) 265
Mly113I GGCGCC 1 cut(s) 109
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 9 cut(s) 127, 139, 154, 166, 187, 239, 285, 313, 440
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 313
MwoI GCNNNNNNNGC 1 cut(s) 46
NarI GGCGCC 1 cut(s) 109
NcoI CCATGG 1 cut(s) 31
NdeII GATC 2 cut(s) 16, 393
NlaIII CATG 4 cut(s) 23, 28, 35, 349
NlaIV GGNNCC 2 cut(s) 41, 110
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 4, 207
NspI RCATGY 2 cut(s) 28, 349
NspV TTCGAA 2 cut(s) 232, 279
PagI TCATGA 1 cut(s) 19
PciI ACATGT 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 313
PkrI GCNGC 2 cut(s) 97, 375
PleI GAGTC 1 cut(s) 181
PluTI GGCGCC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 181
PscI ACATGT 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 41, 110
PspPI GGNCC 2 cut(s) 39, 114
PstI CTGCAG 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 276
SatI GCNGC 2 cut(s) 96, 374
Sau3AI GATC 2 cut(s) 16, 393
Sau96I GGNCC 2 cut(s) 39, 114
SchI GAGTC 1 cut(s) 182
SduI GDGCHC 2 cut(s) 288, 385
SetI ASST 8 cut(s) 66, 89, 97, 146, 165, 209, 253, 324
SfaNI GCATC 1 cut(s) 191
SfcI CTRYAG 1 cut(s) 96
SfoI GGCGCC 1 cut(s) 110
SfuI TTCGAA 2 cut(s) 232, 279
SinI GGWCC 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 1 cut(s) 265
SsiI CCGC 1 cut(s) 132
SspDI GGCGCC 1 cut(s) 108
SspMI CTAG 3 cut(s) 44, 212, 386
StyI CCWWGG 2 cut(s) 31, 43
TaaI ACNGT 1 cut(s) 369
TaiI ACGT 3 cut(s) 66, 209, 253
TaqI TCGA 5 cut(s) 15, 232, 279, 299, 337
TasI AATT 1 cut(s) 265
TatI WGTACW 1 cut(s) 255
TseFI GTSAC 2 cut(s) 4, 207
TseI GCWGC 2 cut(s) 95, 373
Tsp45I GTSAC 2 cut(s) 4, 207
TspDTI ATGAA 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 1 cut(s) 265
XceI RCATGY 2 cut(s) 28, 349
XmaJI CCTAGG 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 326, 342
XspI CTAG 3 cut(s) 44, 212, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.