MD05G1312300.v1.1

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011706
Physical Location & Seq
Reverse (-)
44203223 .. 44205766
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1312300.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGAATCAGGCAGAGGTCATGGAAAGAGGATCTTGGTGAAATACGAGGAAGAGGAAGAGGAGGAGGAGGAAGAAGAAGAAAGTAGGAGTGGGACACCATCTATAGTGGATGGTGAGGATGAGAGGAGAGATACAGTGATGATGATGAATAGTACTGCTCCTCCTCCTACAGGGAGAAGATCTGGTGCAGGAGGGTCTACAGCAGGGCCATGTTGTAAGGTAGATTGTTGCAATGCTGACTTGAGTGATTTAAAGCAATACTACCGCCGCCACAAGGTCTGTGACGTTCATGCCAAGGCTCCGGCGGTGTTCATGGGTGGAATCCGGCAGCGCTTTTGCCAGCAGTGTAGCAGGTTTCACAGCCTATCGGAGTTTGATGACCGTAAAAGGAGTTGTCGCCGGCGATTAGCTGGACACAATGAGCGGCGTCGGAAGACCTCCCTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

150

Amino Acids

16.97

Weight (kDa)

8.13

Isoelectric Point (pI)

93.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 72 - 145 3.7e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011706)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33810
fragaria_vesca FvH4_3g08880
malus_domestica MD05G1312300.v1.1 MD10G1291800.v1.1
prunus_persica Prupe.4G050400_v2.0.a1 Prupe.4G050400_v2.0.a1
pyrus_communis pycom05g28830 pycom10g24400
rosa_chinensis RchiOBHm_Chr5g0008871
rosa_laevigata RLG00000031612
rosa_multiflora Rmu_sc0004677.1_g000008
rosa_roxburghii Rroxscaffold_1G00067130 Rroxscaffold_1G00067750
rosa_rugosa Rorug04G0439900
rosa_samantha Rh5AG068500 Rh5BG065200 Rh5CG076400 Rh5DG064300
rosa_wichuraiana Rw5G006410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 197
AciI CCGC 4 cut(s) 265, 268, 305, 424
AclWI GGATC 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 427
AfaI GTAC 1 cut(s) 154
AfeI AGCGCT 1 cut(s) 332
AfiI CCNNNNNNNGG 2 cut(s) 170, 274
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
AlwI GGATC 1 cut(s) 38
Aor51HI AGCGCT 1 cut(s) 332
AoxI GGCC 1 cut(s) 206
ApeKI GCWGC 1 cut(s) 328
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 49, 125
BbsI GAAGAC 1 cut(s) 440
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 104, 106
BfmI CTRYAG 3 cut(s) 102, 168, 198
BfoI RGCGCY 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 342
BglII AGATCT 1 cut(s) 179
BisI GCNGC 3 cut(s) 268, 329, 425
BlsI GCNGC 3 cut(s) 269, 330, 426
BmcAI AGTACT 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 427
BsaJI CCNNGG 1 cut(s) 294
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 274
Bse118I RCCGGY 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 2 cut(s) 115, 124
BseLI CCNNNNNNNGG 2 cut(s) 170, 274
BseMI GCAATG 1 cut(s) 238
BseRI GAGGAG 6 cut(s) 74, 77, 80, 139, 150, 153
BseXI GCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 1 cut(s) 208
BsiSI CCGG 3 cut(s) 302, 325, 400
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 2 cut(s) 170, 274
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 30, 179
BspACI CCGC 4 cut(s) 265, 268, 305, 424
BspANI GGCC 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 300
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 2 cut(s) 30, 179
BssNI GRCGYC 1 cut(s) 427
BssT1I CCWWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 136, 383
Bst6I CTCTTC 2 cut(s) 45, 51
BstACI GRCGYC 1 cut(s) 427
BstC8I GCNNGC 2 cut(s) 341, 401
BstENI CCTNNNNNAGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 115, 124
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 2 cut(s) 33, 182
BstMBI GATC 2 cut(s) 30, 179
BstSFI CTRYAG 3 cut(s) 102, 168, 198
BstV1I GCAGC 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 440
BstX2I RGATCY 2 cut(s) 30, 179
BstYI RGATCY 2 cut(s) 30, 179
BsuRI GGCC 1 cut(s) 208
BtsCI GGATG 2 cut(s) 115, 124
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 141, 350
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 2 cut(s) 341, 401
CfoI GCGC 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 153
CviAII CATG 4 cut(s) 20, 210, 290, 313
CviJI RGCY 4 cut(s) 208, 299, 363, 410
CviKI_1 RGCY 4 cut(s) 208, 299, 363, 410
CviQI GTAC 1 cut(s) 153
DpnI GATC 2 cut(s) 32, 181
DpnII GATC 2 cut(s) 30, 179
DraI TTTAAA 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 45, 51
EarI CTCTTC 2 cut(s) 45, 51
Eco130I CCWWGG 1 cut(s) 294
Eco47III AGCGCT 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 4 cut(s) 23, 213, 293, 316
FaiI YATR 5 cut(s) 21, 104, 211, 291, 314
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 1 cut(s) 106
FatI CATG 4 cut(s) 19, 209, 289, 312
FblI GTMKAC 1 cut(s) 197
Fnu4HI GCNGC 3 cut(s) 268, 329, 425
FokI GGATG 2 cut(s) 122, 131
Fsp4HI GCNGC 3 cut(s) 268, 329, 425
GlaI GCGC 1 cut(s) 332
GluI GCNGC 3 cut(s) 268, 329, 425
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 1 cut(s) 208
HapII CCGG 3 cut(s) 302, 325, 400
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 427
Hin1II CATG 4 cut(s) 23, 213, 293, 316
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HinfI GANTC 2 cut(s) 5, 321
HpaII CCGG 3 cut(s) 302, 325, 400
HphI GGTGA 2 cut(s) 49, 125
Hpy166II GTNNAC 1 cut(s) 198
Hpy188I TCNGA 2 cut(s) 370, 432
Hpy8I GTNNAC 1 cut(s) 198
Hpy99I CGWCG 1 cut(s) 432
HpyCH4III ACNGT 2 cut(s) 136, 383
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 2 cut(s) 188, 231
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 427
Hsp92II CATG 4 cut(s) 23, 213, 293, 316
HspAI GCGC 1 cut(s) 331
KroI GCCGGC 1 cut(s) 399
KroNI GCCGGC 1 cut(s) 401
Kzo9I GATC 2 cut(s) 30, 179
LmnI GCTCC 2 cut(s) 163, 304
Lsp1109I GCAGC 1 cut(s) 340
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 281
MalI GATC 2 cut(s) 32, 181
MbiI CCGCTC 1 cut(s) 424
MboI GATC 2 cut(s) 30, 179
MboII GAAGA 7 cut(s) 62, 68, 83, 86, 89, 189, 445
MflI RGATCY 2 cut(s) 30, 179
MmeI TCCRAC 1 cut(s) 410
MreI CGCCGGCG 1 cut(s) 399
MroNI GCCGGC 1 cut(s) 399
MseI TTAA 1 cut(s) 251
MspI CCGG 3 cut(s) 302, 325, 400
NaeI GCCGGC 1 cut(s) 401
NdeII GATC 2 cut(s) 30, 179
NgoMIV GCCGGC 1 cut(s) 399
NlaIII CATG 4 cut(s) 23, 213, 293, 316
NlaIV GGNNCC 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 281
PdiI GCCGGC 1 cut(s) 401
PfeI GAWTC 2 cut(s) 5, 321
PkrI GCNGC 3 cut(s) 269, 330, 426
PspN4I GGNNCC 1 cut(s) 300
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 2 cut(s) 30, 179
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
SaqAI TTAA 1 cut(s) 251
SatI GCNGC 3 cut(s) 268, 329, 425
Sau3AI GATC 2 cut(s) 30, 179
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 1 cut(s) 154
SetI ASST 7 cut(s) 19, 222, 279, 288, 356, 412, 440
SfcI CTRYAG 3 cut(s) 102, 168, 198
SgrAI CRCCGGYG 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
SsiI CCGC 4 cut(s) 265, 268, 305, 424
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 136, 383
TaiI ACGT 1 cut(s) 288
TatI WGTACW 1 cut(s) 152
TauI GCSGC 2 cut(s) 270, 427
TfiI GAWTC 2 cut(s) 5, 321
Tru1I TTAA 1 cut(s) 251
Tru9I TTAA 1 cut(s) 251
TscAI CASTG 2 cut(s) 141, 350
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 1 cut(s) 328
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 3 cut(s) 161, 278, 301
TspRI CASTG 2 cut(s) 141, 350
XagI CCTNNNNNAGG 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 197
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.