Rroxscaffold_1G00067750

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011706
Physical Location & Seq
Reverse (-)
89005188 .. 89006798
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067750.1

Sequence Viewer

Length: 468 bp
ATGGAACCAAACAAAGCTCAAGGAAAGAGGAGCTTGAACTTCAAGACTGAGGAGGAGGAAGAGGAAGAAGAAGAGGATGAGGACGAACAGCATGCGAGTACGGAACTACTGCATGGAGAAGATGACAGGAGAAAAAGAGTGATGGTGATGAATAGTACTAGCTCTGCTAGTCCAAGCAAGAAAGGATCCGGTGCAGGGGGATCTACGACGACAAGGCCGTCTTGCCAAGCGCAGAGTTGCAATGCAGACCTGAGTGATGCAAAGCAATACTACCGCCGCCACAAGGTCTGCGATTTTCATTCCAAGGCTCCGGCGGTGATCGTCGGTGGTCTCCAACAGCGGTTTTGCCAGCAATGTAGCAGGTTTCATAACCTGTCAGAGTTCGATGAAAACAAAAGGAGTTGTCGCAGACGTTTAGCTGGGCACAACGAACGGCGCCGGAAAAGCTCCGCCGATTATCCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

155

Amino Acids

17.64

Weight (kDa)

7.65

Isoelectric Point (pI)

101.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 75 - 148 2.3e-33 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011706)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33810
fragaria_vesca FvH4_3g08880
malus_domestica MD05G1312300.v1.1 MD10G1291800.v1.1
prunus_persica Prupe.4G050400_v2.0.a1 Prupe.4G050400_v2.0.a1
pyrus_communis pycom05g28830 pycom10g24400
rosa_chinensis RchiOBHm_Chr5g0008871
rosa_laevigata RLG00000031612
rosa_multiflora Rmu_sc0004677.1_g000008
rosa_roxburghii Rroxscaffold_1G00067130 Rroxscaffold_1G00067750
rosa_rugosa Rorug04G0439900
rosa_samantha Rh5AG068500 Rh5BG065200 Rh5CG076400 Rh5DG064300
rosa_wichuraiana Rw5G006410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 435
AciI CCGC 5 cut(s) 274, 277, 314, 340, 450
AclWI GGATC 3 cut(s) 180, 193, 208
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 2 cut(s) 100, 157
AfiI CCNNNNNNNGG 2 cut(s) 195, 283
AgsI TTSAA 2 cut(s) 37, 43
AluBI AGCT 5 cut(s) 17, 33, 162, 419, 447
AluI AGCT 5 cut(s) 17, 33, 162, 419, 447
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 3 cut(s) 180, 193, 208
AoxI GGCC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 232, 438
AsuHPI GGTGA 2 cut(s) 157, 328
BaeGI GKGCMC 1 cut(s) 426
BamHI GGATCC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 435
BccI CCATC 1 cut(s) 136
BceAI ACGGC 2 cut(s) 202, 449
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 2 cut(s) 159, 168
BfoI RGCGCY 1 cut(s) 439
BfuAI ACCTGC 1 cut(s) 351
BisI GCNGC 1 cut(s) 277
BlsI GCNGC 1 cut(s) 278
BmcAI AGTACT 1 cut(s) 157
BmiI GGNNCC 4 cut(s) 6, 187, 309, 437
BmsI GCATC 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 436
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 283
Bse3DI GCAATG 2 cut(s) 247, 359
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 2 cut(s) 195, 283
BseMI GCAATG 2 cut(s) 247, 359
BseMII CTCAG 2 cut(s) 39, 242
BseRI GAGGAG 3 cut(s) 43, 65, 68
BseSI GKGCMC 1 cut(s) 426
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 1 cut(s) 217
BshNI GGYRCC 1 cut(s) 435
BsiSI CCGG 3 cut(s) 189, 311, 439
BslI CCNNNNNNNGG 2 cut(s) 195, 283
BsmAI GTCTC 1 cut(s) 335
BsnI GGCC 1 cut(s) 217
Bso31I GGTCTC 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 3 cut(s) 185, 200, 318
BspACI CCGC 5 cut(s) 274, 277, 314, 340, 450
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 2 cut(s) 40, 243
BspLI GGNNCC 4 cut(s) 6, 187, 309, 437
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 3 cut(s) 180, 193, 208
BspT107I GGYRCC 1 cut(s) 435
BspTNI GGTCTC 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 247, 359
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 3 cut(s) 185, 200, 318
BssNI GRCGYC 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 303
Bst6I CTCTTC 2 cut(s) 54, 66
BstACI GRCGYC 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 93, 350
BstDEI CTNAG 2 cut(s) 48, 251
BstF5I GGATG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 2 cut(s) 232, 438
BstKTI GATC 3 cut(s) 188, 203, 321
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 3 cut(s) 185, 200, 318
BstMWI GCNNNNNNNGC 1 cut(s) 444
BstNSI RCATGY 1 cut(s) 95
BstSLI GKGCMC 1 cut(s) 426
BstX2I RGATCY 2 cut(s) 185, 200
BstYI RGATCY 2 cut(s) 185, 200
BsuRI GGCC 1 cut(s) 217
BtsCI GGATG 1 cut(s) 82
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 93, 350
CfoI GCGC 2 cut(s) 232, 438
Csp6I GTAC 2 cut(s) 99, 156
CviAII CATG 2 cut(s) 92, 113
CviJI RGCY 7 cut(s) 17, 33, 162, 217, 308, 419, 447
CviKI_1 RGCY 7 cut(s) 17, 33, 162, 217, 308, 419, 447
CviQI GTAC 2 cut(s) 99, 156
DdeI CTNAG 2 cut(s) 48, 251
DinI GGCGCC 1 cut(s) 437
DpnI GATC 3 cut(s) 187, 202, 320
DpnII GATC 3 cut(s) 185, 200, 318
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 54, 66
EarI CTCTTC 2 cut(s) 54, 66
EciI GGCGGA 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 303
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 2 cut(s) 95, 116
FaiI YATR 3 cut(s) 93, 114, 369
FalI AAGNNNNNCTT 4 cut(s) 17, 49, 205, 237
FatI CATG 2 cut(s) 91, 112
Fnu4HI GCNGC 1 cut(s) 277
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 277
FspBI CTAG 2 cut(s) 159, 168
GlaI GCGC 2 cut(s) 231, 437
GluI GCNGC 1 cut(s) 277
GsaI CCCAGC 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 217
HapII CCGG 3 cut(s) 189, 311, 439
HhaI GCGC 2 cut(s) 232, 438
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 2 cut(s) 95, 116
Hin6I GCGC 2 cut(s) 230, 436
HinP1I GCGC 2 cut(s) 230, 436
HpaII CCGG 3 cut(s) 189, 311, 439
HphI GGTGA 2 cut(s) 157, 328
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 1 cut(s) 43
Hpy99I CGWCG 2 cut(s) 211, 326
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 5 cut(s) 112, 194, 240, 245, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 444
HpyF3I CTNAG 2 cut(s) 48, 251
HpySE526I ACGT 1 cut(s) 412
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 2 cut(s) 95, 116
HspAI GCGC 2 cut(s) 230, 436
KasI GGCGCC 1 cut(s) 435
Kzo9I GATC 3 cut(s) 185, 200, 318
LmnI GCTCC 3 cut(s) 30, 313, 452
LweI GCATC 1 cut(s) 247
MaeI CTAG 2 cut(s) 159, 168
MaeII ACGT 1 cut(s) 412
MalI GATC 3 cut(s) 187, 202, 320
MboI GATC 3 cut(s) 185, 200, 318
MboII GAAGA 5 cut(s) 71, 77, 80, 83, 131
MflI RGATCY 2 cut(s) 185, 200
MhlI GDGCHC 1 cut(s) 426
Mly113I GGCGCC 1 cut(s) 436
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 7 cut(s) 21, 43, 46, 49, 55, 67, 73
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 3 cut(s) 189, 311, 439
MwoI GCNNNNNNNGC 1 cut(s) 444
NarI GGCGCC 1 cut(s) 436
NdeII GATC 3 cut(s) 185, 200, 318
NlaIII CATG 2 cut(s) 95, 116
NlaIV GGNNCC 4 cut(s) 6, 187, 309, 437
NspI RCATGY 1 cut(s) 95
PaeI GCATGC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PkrI GCNGC 1 cut(s) 278
PluTI GGCGCC 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 419
PspN4I GGNNCC 4 cut(s) 6, 187, 309, 437
PsuI RGATCY 2 cut(s) 185, 200
RsaI GTAC 2 cut(s) 100, 157
RsaNI GTAC 2 cut(s) 99, 156
SatI GCNGC 1 cut(s) 277
Sau3AI GATC 3 cut(s) 185, 200, 318
ScaI AGTACT 1 cut(s) 157
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 1 cut(s) 247
SfoI GGCGCC 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
SphI GCATGC 1 cut(s) 95
SsiI CCGC 5 cut(s) 274, 277, 314, 340, 450
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 2 cut(s) 159, 168
StyI CCWWGG 1 cut(s) 303
TaiI ACGT 1 cut(s) 415
TaqI TCGA 1 cut(s) 384
TatI WGTACW 1 cut(s) 155
TauI GCSGC 1 cut(s) 279
TspDTI ATGAA 4 cut(s) 164, 287, 356, 402
TspGWI ACGGA 1 cut(s) 116
XceI RCATGY 1 cut(s) 95
XspI CTAG 2 cut(s) 159, 168
ZrmI AGTACT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.