MD10G1291800.v1.1

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011706
Physical Location & Seq
Reverse (-)
38022115 .. 38024690
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1291800.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGATTCAGGCAGAGCTCATGGAAAGAGGACCATGTCGAAATACGAGGTGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGGAAGAAGAAAGTAGGAGTAGGAGACCATCAATAGTGTATGGTGAGGATGAGGGGAGAGCAAGAGTGACGATGGTGAATAGTACTGCTGCTACTCCTACAGGGAGAAGATCTGGTGCAGGAGGGTCTACGGCAGGGCCATGTTGTCAGGTAGATAATTGCACTGCTGACTTGAGTGATTTAAAGCAATACTACCGCCGCCACAAGGTCTGTGATGTTCATGCCAAGGCTCCGTCGATAGTCGTGGGTGGATTCCGGCAGCGTTTTTGCCAGCAATGTAGCAGGTTTCATAGCCTATCAGAGTTTGATGACAGTAAAAGGAGTTGTCGCAGGCGATTAGCTGGACACAATGAGCGGCGTCGGAAAACTTCATCGGAATACCATGGACATGGAGGCACGTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

169

Amino Acids

19.05

Weight (kDa)

5.71

Isoelectric Point (pI)

103.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 82 - 155 4.4e-34 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011706)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33810
fragaria_vesca FvH4_3g08880
malus_domestica MD05G1312300.v1.1 MD10G1291800.v1.1
prunus_persica Prupe.4G050400_v2.0.a1 Prupe.4G050400_v2.0.a1
pyrus_communis pycom05g28830 pycom10g24400
rosa_chinensis RchiOBHm_Chr5g0008871
rosa_laevigata RLG00000031612
rosa_multiflora Rmu_sc0004677.1_g000008
rosa_roxburghii Rroxscaffold_1G00067130 Rroxscaffold_1G00067750
rosa_rugosa Rorug04G0439900
rosa_samantha Rh5AG068500 Rh5BG065200 Rh5CG076400 Rh5DG064300
rosa_wichuraiana Rw5G006410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 227
AciI CCGC 3 cut(s) 295, 298, 454
AcyI GRCGYC 1 cut(s) 457
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 1 cut(s) 304
AjiI CACGTC 1 cut(s) 498
AluBI AGCT 2 cut(s) 17, 440
AluI AGCT 2 cut(s) 17, 440
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 118
AoxI GGCC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 188, 358
AspS9I GGNCC 2 cut(s) 30, 236
AsuHPI GGTGA 2 cut(s) 155, 187
AvaII GGWCC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 175, 370
BccI CCATC 2 cut(s) 136, 166
BceAI ACGGC 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 118
BfmI CTRYAG 1 cut(s) 198
BfuAI ACCTGC 1 cut(s) 372
BglII AGATCT 1 cut(s) 209
BisI GCNGC 4 cut(s) 189, 298, 359, 455
BlsI GCNGC 4 cut(s) 190, 299, 360, 456
BmcAI AGTACT 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 30
BmgBI CACGTC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 30, 236
BmiI GGNNCC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 457
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 324, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 380
BseDI CCNNGG 2 cut(s) 324, 481
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 175, 370
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 1 cut(s) 355
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 1 cut(s) 238
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 209
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 3 cut(s) 295, 298, 454
BspANI GGCC 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 118
BsrBI CCGCTC 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 324, 481
BssMI GATC 1 cut(s) 209
BssNI GRCGYC 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 324, 481
Bst4CI ACNGT 1 cut(s) 413
Bst6I CTCTTC 3 cut(s) 60, 75, 81
BstACI GRCGYC 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 371, 431
BstDSI CCRYGG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 209
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 175, 370
BstX2I RGATCY 1 cut(s) 209
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 1 cut(s) 238
BtgI CCRYGG 1 cut(s) 481
BtrI CACGTC 1 cut(s) 498
BtsCI GGATG 1 cut(s) 154
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 261
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 2 cut(s) 371, 431
Cfr13I GGNCC 2 cut(s) 30, 236
CseI GACGC 1 cut(s) 446
Csp6I GTAC 1 cut(s) 183
CviAII CATG 6 cut(s) 20, 34, 240, 320, 482, 488
CviJI RGCY 5 cut(s) 17, 238, 329, 393, 440
CviKI_1 RGCY 5 cut(s) 17, 238, 329, 393, 440
CviQI GTAC 1 cut(s) 183
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
DraI TTTAAA 1 cut(s) 282
Eam1104I CTCTTC 3 cut(s) 60, 75, 81
EarI CTCTTC 3 cut(s) 60, 75, 81
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 324, 481
Eco24I GRGCYC 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoT14I CCWWGG 2 cut(s) 324, 481
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 2 cut(s) 324, 481
FaeI CATG 6 cut(s) 23, 37, 243, 323, 485, 491
FaiI YATR 8 cut(s) 21, 35, 141, 241, 321, 390, 483, 489
FatI CATG 6 cut(s) 19, 33, 239, 319, 481, 487
FblI GTMKAC 1 cut(s) 227
Fnu4HI GCNGC 4 cut(s) 189, 298, 359, 455
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 4 cut(s) 189, 298, 359, 455
GluI GCNGC 4 cut(s) 189, 298, 359, 455
HaeIII GGCC 1 cut(s) 238
HapII CCGG 1 cut(s) 355
HgaI GACGC 1 cut(s) 446
Hin1I GRCGYC 1 cut(s) 457
Hin1II CATG 6 cut(s) 23, 37, 243, 323, 485, 491
HinfI GANTC 2 cut(s) 5, 351
HpaII CCGG 1 cut(s) 355
HphI GGTGA 2 cut(s) 155, 187
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 3 cut(s) 400, 462, 475
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 2 cut(s) 337, 462
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 2 cut(s) 218, 261
HpySE526I ACGT 1 cut(s) 497
Hsp92I GRCGYC 1 cut(s) 457
Hsp92II CATG 6 cut(s) 23, 37, 243, 323, 485, 491
Kzo9I GATC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 2 cut(s) 175, 370
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 166
MalI GATC 1 cut(s) 211
MbiI CCGCTC 1 cut(s) 454
MboI GATC 1 cut(s) 209
MboII GAAGA 6 cut(s) 77, 92, 98, 116, 119, 219
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 2 cut(s) 256, 502
MmeI TCCRAC 1 cut(s) 440
MseI TTAA 2 cut(s) 281, 505
MslI CAYNNNNRTG 1 cut(s) 486
MspI CCGG 1 cut(s) 355
NcoI CCATGG 1 cut(s) 481
NdeII GATC 1 cut(s) 209
NlaIII CATG 6 cut(s) 23, 37, 243, 323, 485, 491
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 166
PfeI GAWTC 2 cut(s) 5, 351
PflFI GACNNNGTC 1 cut(s) 34
PkrI GCNGC 4 cut(s) 190, 299, 360, 456
Psp124BI GAGCTC 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 30, 236
PsuI RGATCY 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 34
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 486
SacI GAGCTC 1 cut(s) 19
SaqAI TTAA 2 cut(s) 281, 505
SatI GCNGC 4 cut(s) 189, 298, 359, 455
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 2 cut(s) 30, 236
ScaI AGTACT 1 cut(s) 184
SduI GDGCHC 1 cut(s) 19
SetI ASST 7 cut(s) 19, 51, 252, 309, 386, 442, 500
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 2 cut(s) 256, 502
SsiI CCGC 3 cut(s) 295, 298, 454
SstI GAGCTC 1 cut(s) 19
StyI CCWWGG 2 cut(s) 324, 481
TaaI ACNGT 1 cut(s) 413
TaiI ACGT 1 cut(s) 500
TaqI TCGA 2 cut(s) 38, 335
TasI AATT 2 cut(s) 256, 502
TatI WGTACW 1 cut(s) 182
TauI GCSGC 2 cut(s) 300, 457
TfiI GAWTC 2 cut(s) 5, 351
Tru1I TTAA 2 cut(s) 281, 505
Tru9I TTAA 2 cut(s) 281, 505
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 188, 358
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 3 cut(s) 308, 377, 459
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 1 cut(s) 268
Tth111I GACNNNGTC 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 227
ZrmI AGTACT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.