Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007917

Physical Location & Seq

Genomic Coordinates Forward (+)

45437425 .. 45441443

Gene Structure 4019 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1329600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGGGAATCGGTAGCAAAAACCCAGGTTCCAGAATTTGTATTTTCTCATTCGTTTTCTGCTGCTATCTTCCTCTGTTTTGCTCTGCGTCGAGTTCGATTTCGCAGGGCCAGTCTATACAAGATGGAGAAACGCTGATCTCAGATGGGCAAATTTTCGAACTGGGTTTCTTCCGTCCGGGGAATTCATCGTTCCGGTACGTCGGAATTCGGTATTACAACATTTCAGACCCGCCAGTGATCTGGGTTGCAAACAGAGAAAACCCAATTTCTGGTGAATCTGGAGTTTTGAAAATTGGAAGCGATGGAAATCTGGTTGTTTTGGATGGAAATGGCACCGCAGTTTGGTCTACTAACGCTTCAACCGCCACTAACACGACTGCCACTAACACGACCGCCACACTCAGTGACGAAGGAAGTCTTGTTCTTTCCGGCAGCGGCGATGCCGGCGAGGTCTACTGGCAGAGCTTTGATCATCCAGCGGACACTTTTTTGCCGGGGATGAAAGTTGAGGTGAATGATGCCATCGGAGAGAATCGGTTTTTGAGTTCGTGGAAGTCGGAAAGTGACCCTTCGGCCGGAGGTTACTCAATGGGCGTTGATCCTCGAGGATCGCCGCAGTTTGTGATATGGGAAGGATCAGAACGGCGGTGGCGAAGCGGGCATTGGAACAAGCAGATATTCATCGGAATGCCGACTATGCCAACGAATTATGAATCTGGTTTTAGGCTTAGTGATGAGAATGCGAATGGGAGCTCATATTTTACTTACAAACTATCAAGTACTTCGGAGAAGTTGAGGTTTCGGATAAGTTGGGACGGGTACGAGGAGCTGCTGAGATGGGTGGAAGAGAAGAACCAGTGGGAAGTGATAGAATCTCAGCCTAACAAGACCAACCAGTGTGAGTTTTACAACAAATGTGGAAAGTTTGGGGTGTGTAGTGCATCCGATGATTCGGGTTCGATTTGCAGTTGTATGCATGGATTTCAACCTAGGGACTCGGATCAATGGATGAAGGGAAATTGGTCGGAGGGGTGTTTGAGGAAAACCTTATTGCAGTGTCAGAGAAATAGTACTAATGGAACGGCGGCAAGTGATGAAAAAGATGGATTTGTAGGGATAAGAGGTGCCAAGTTGCCAGATTTCGCAGACTTAGTTAACGCAGGTCCAGGGGAGGGTTGTGAGGAGAAATGTCTTGTGAATTGTTCTTGTACTGCTTATGCATTTGTTGAAGGGATTGGTTGTATGATGTGGACCGGGGACTTGATTGACGTCGAACAATTTACATTGGGAGGAAACACGTTGCAAATTCGTGTTGCGCATTCGGATTTAGGTAACAAGAAAAAATTATCCACCGTTGTGATAGCAGTAATTTCAGTAGCGGGAGCACTATTCGTAGTCATCATCCTATTGCTTTTGTGGTGGTTCAAAGCAAAACTGAAAGTGCTGCCCATAAGTTCTTCAATTTCATGGTTAAGGGGCAGTGATACACCAATGCTCGATGCTGGAAAGAGTGAAGAATTCTCAACAGATACTTCAGGATCAGTTAACGTTTTTGCACAAGGGAATCAAGCAAATGGATCCGAATTACCGCTGTTCAATTTCAGTTGTGTAGCAGCTGCTACGAACAACTTTTCTGAAGAAAACAAGCTTGGGAAGGGGGGATTTGGTACTGTCTACAAGGGTAGCCTTCCAGGGCTACCACAAATAGCCGTAAAAAGACTTTCGAGAAGGTCTACTCAAGGGTTGGAGGAGTTCAAGAACGAGATTAGTCTGATTGCTAAGTTACAACACAGGAATCTCGTGAGGCTATTGGGCTGCTGCATTGAAGGAGAAGAAAAGATACTGGTTTATGAATACATGCCAAACAAAAGCTTGGATTTCTTTCTTTTCCATCCAGCCAACCGATCACTACTAGATTGGAGAAAACGCTTTACGATTATTGAAGGGATTGCACGAGGGCTCCTTTATCTCCATCGAGATTCAAGACTTAGAATTATTCATCGAGACCTAAAAGCTAGCAACATTTTGCTGGATGAAGATATGATCCCCAAAATTTCAGACTTTGGCATGGCAAGAATATTCGGGGGTAATGAAAAAGAAGAAAATACAGCCCGAGTTGTTGGCACCTACGGTTATATGTCACCTGAATATGCAATGGAAGGTCTGTTTTCGGTAAAGTCAGATGTTTATAGCTTTGGTGTATTGCTGCTAGAGATTGTGAGTGGCCGAAGGAACACTAGCTTGCGATCAACGGAACACTTAAGCCTTATTGGATATGCATGGCATCTTTGGAACGAGAATCGAGCAACGGACCTCATTGATCCTTCCATCGCAGAAACATGTTCCCAGAACGAAACTGAAGTATTGAGATGTATACATGTAGGGTTACTGTGTGTGCAAGACCATGCAGCTAGTAGACCAACCATGTCGGCCGTGGTGTTAATGCTGGAAAGTGAAGCTGCAAATCTTCAGTTGCCAGTAGAACCTATATTTACCTCAATTAAGAGACATGTGGTTGTGGATACAAAATTTAGTACAGAAGGCCAGGATATAGCACCCTCGAATGATGTAACGATTACAATGGTGGAAGGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

866

Amino Acids

95.41

Weight (kDa)

5.35

Isoelectric Point (pI)

42.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	79 - 185	1.1e-35	D-mannose binding lectin
S_locus_glycop	PF00954	217 - 331	6.1e-26	S-locus glycoprotein domain
PAN_2	PF08276	365 - 424	7.9e-17	PAN-like domain
Pkinase	PF00069	545 - 749	5.9e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	547 - 813	3.7e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007917)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61610 AT1G61610 AT4G21390 AT4G21390
fragaria_vesca	FvH4_3g03590 FvH4_3g03590 FvH4_3g03590 FvH4_3g03590 FvH4_3g03590
malus_domestica	MD05G1329600.v1.1 MD10G1306600.v1.1
prunus_persica	Prupe.4G033200_v2.0.a1
pyrus_communis	pycom05g30270 pycom10g25870
rosa_chinensis	RchiOBHm_Chr5g0005641
rosa_laevigata	RLG00000031314
rosa_multiflora	Rmu_co8421279.1_g000001
rosa_roxburghii	Rroxscaffold_1G00070190
rosa_rugosa	Rorug04G0417000
rosa_samantha	Rh5AG044400 Rh5BG043200 Rh5CG052000 Rh5DG043200
rosa_wichuraiana	Rw5G004470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1272
AbsI	CCTCGAGG	1 cut(s)	603
Acc16I	TGCGCA	1 cut(s)	1317
Acc36I	ACCTGC	1 cut(s)	1151
AccB1I	GGYRCC	3 cut(s)	332, 1124, 2123
AccB7I	CCANNNNNTGG	1 cut(s)	269
AccI	GTMKAC	6 cut(s)	347, 453, 1674, 1733, 2374, 2416
AclI	AACGTT	1 cut(s)	1548
AclWI	GGATC	9 cut(s)	593, 616, 643, 1008, 1546, 1572, 1585, 2038, 2315
AcoI	YGGCCR	3 cut(s)	573, 2224, 2430
AcsI	RAATTY	8 cut(s)	33, 150, 181, 204, 1305, 1517, 2052, 2528
AcuI	CTGAAG	4 cut(s)	1518, 1656, 2379, 2453
AcyI	GRCGYC	1 cut(s)	1269
AdeI	CACNNNGTG	1 cut(s)	404
AfaI	GTAC	7 cut(s)	197, 781, 821, 1072, 1210, 1669, 2536
AfiI	CCNNNNNNNGG	4 cut(s)	269, 342, 575, 1172
AflII	CTTAAG	1 cut(s)	2260
AflIII	ACRYGT	4 cut(s)	1296, 2339, 2377, 2509
AjnI	CCWGG	4 cut(s)	22, 1165, 1690, 2544
AjuI	GAANNNNNNNTTGG	4 cut(s)	646, 678, 1632, 1664
AleI	CACNNNNGTG	1 cut(s)	1355
AloI	GAACNNNNNNTCC	2 cut(s)	405, 437
Alw21I	GWGCWC	2 cut(s)	755, 1387
Alw26I	GTCTC	2 cut(s)	1998, 2500
AlwI	GGATC	9 cut(s)	593, 616, 643, 1008, 1546, 1572, 1585, 2038, 2315
Ama87I	CYCGRG	2 cut(s)	603, 2112
AoxI	GGCC	5 cut(s)	106, 573, 2224, 2430, 2542
ApoI	RAATTY	8 cut(s)	33, 150, 181, 204, 1305, 1517, 2052, 2528
AspA2I	CCTAGG	1 cut(s)	989
AspLEI	GCGC	1 cut(s)	1318
AspS9I	GGNCC	4 cut(s)	106, 1163, 1251, 2311
AsuC2I	CCSGG	3 cut(s)	177, 495, 1255
AsuHPI	GGTGA	3 cut(s)	284, 523, 2133
AsuII	TTCGAA	1 cut(s)	156
AsuNHI	GCTAGC	1 cut(s)	2015
AvaI	CYCGRG	2 cut(s)	603, 2112
AvaII	GGWCC	3 cut(s)	1163, 1251, 2311
AvrII	CCTAGG	1 cut(s)	989
BamHI	GGATCC	1 cut(s)	1577
BanI	GGYRCC	3 cut(s)	332, 1124, 2123
BanII	GRGCYC	2 cut(s)	755, 1962
BarI	GAAGNNNNNNTAC	2 cut(s)	1824, 1856
BauI	CACGAG	2 cut(s)	1799, 1953
Bbv12I	GWGCWC	2 cut(s)	755, 1387
BceAI	ACGGC	4 cut(s)	659, 1098, 1694, 2417
BciT130I	CCWGG	4 cut(s)	24, 1167, 1692, 2546
BciVI	GTATCC	1 cut(s)	2515
BclI	TGATCA	1 cut(s)	469
BcnI	CCSGG	3 cut(s)	177, 495, 1255
BcoDI	GTCTC	2 cut(s)	1998, 2500
BfaI	CTAG	6 cut(s)	990, 1913, 2016, 2210, 2238, 2412
BfrI	CTTAAG	1 cut(s)	2260
BfuAI	ACCTGC	1 cut(s)	1151
BfuI	GTATCC	1 cut(s)	2515
BlnI	CCTAGG	1 cut(s)	989
BmcAI	AGTACT	2 cut(s)	781, 1072
Bme1390I	CCNGG	7 cut(s)	24, 177, 495, 1167, 1255, 1692, 2546
Bme18I	GGWCC	3 cut(s)	1163, 1251, 2311
BmeT110I	CYCGRG	2 cut(s)	603, 2112
BmgT120I	GGNCC	4 cut(s)	106, 1163, 1251, 2311
BmiI	GGNNCC	6 cut(s)	28, 334, 1126, 1579, 1961, 2125
BmrFI	CCNGG	7 cut(s)	24, 177, 495, 1167, 1255, 1692, 2546
BmrI	ACTGGG	1 cut(s)	170
BmsI	GCATC	5 cut(s)	430, 508, 950, 1489, 2293
BmtI	GCTAGC	1 cut(s)	2019
BmuI	ACTGGG	1 cut(s)	170
BpmI	CTGGAG	1 cut(s)	300
Bpu14I	TTCGAA	1 cut(s)	156
BpuEI	CTTGAG	1 cut(s)	1722
BpuMI	CCSGG	3 cut(s)	177, 495, 1255
BsaBI	GATNNNNATC	2 cut(s)	306, 680
BsaHI	GRCGYC	1 cut(s)	1269
BsaI	GGTCTC	1 cut(s)	1998
BsaJI	CCNNGG	8 cut(s)	22, 176, 494, 989, 1166, 1254, 1691, 2433
BsaWI	WCCGGW	1 cut(s)	192
BsaXI	ACNNNNNCTCC	2 cut(s)	1944, 1974
Bsc4I	CCNNNNNNNGG	4 cut(s)	269, 342, 575, 1172
Bse118I	RCCGGY	1 cut(s)	443
Bse1I	ACTGG	8 cut(s)	109, 165, 233, 461, 856, 895, 1848, 2477
Bse3DI	GCAATG	1 cut(s)	2160
Bse8I	GATNNNNATC	2 cut(s)	306, 680
BseBI	CCWGG	4 cut(s)	24, 1167, 1692, 2546
BseDI	CCNNGG	8 cut(s)	22, 176, 494, 989, 1166, 1254, 1691, 2433
BseGI	GGATG	8 cut(s)	328, 472, 504, 941, 1014, 1401, 1891, 2038
BseJI	GATNNNNATC	2 cut(s)	306, 680
BseLI	CCNNNNNNNGG	4 cut(s)	269, 342, 575, 1172
BseMI	GCAATG	1 cut(s)	2160
BseMII	CTCAG	4 cut(s)	153, 415, 824, 890
BseNI	ACTGG	8 cut(s)	109, 165, 233, 461, 856, 895, 1848, 2477
BseRI	GAGGAG	3 cut(s)	839, 1196, 1763
BseX3I	CGGCCG	2 cut(s)	573, 2430
Bsh1285I	CGRYCG	3 cut(s)	393, 576, 2433
BshFI	GGCC	5 cut(s)	108, 575, 2226, 2432, 2544
BshNI	GGYRCC	3 cut(s)	332, 1124, 2123
BsiEI	CGRYCG	3 cut(s)	393, 576, 2433
BsiHKAI	GWGCWC	2 cut(s)	755, 1387
BsiHKCI	CYCGRG	2 cut(s)	603, 2112
BsiSI	CCGG	7 cut(s)	176, 193, 429, 444, 494, 576, 1254
BslFI	GGGAC	3 cut(s)	827, 1007, 1271
BslI	CCNNNNNNNGG	4 cut(s)	269, 342, 575, 1172
BsmAI	GTCTC	2 cut(s)	1998, 2500
BsmFI	GGGAC	3 cut(s)	827, 1007, 1271
BsmI	GAATGC	3 cut(s)	693, 745, 1318
BsnI	GGCC	5 cut(s)	108, 575, 2226, 2432, 2544
Bso31I	GGTCTC	1 cut(s)	1998
BsoBI	CYCGRG	2 cut(s)	603, 2112
Bsp119I	TTCGAA	1 cut(s)	156
Bsp1286I	GDGCHC	3 cut(s)	755, 1387, 1962
BspANI	GGCC	5 cut(s)	108, 575, 2226, 2432, 2544
BspCNI	CTCAG	4 cut(s)	152, 414, 825, 889
BspLI	GGNNCC	6 cut(s)	28, 334, 1126, 1579, 1961, 2125
BspMI	ACCTGC	1 cut(s)	1151
BspOI	GCTAGC	1 cut(s)	2019
BspPI	GGATC	9 cut(s)	593, 616, 643, 1008, 1546, 1572, 1585, 2038, 2315
BspT104I	TTCGAA	1 cut(s)	156
BspT107I	GGYRCC	3 cut(s)	332, 1124, 2123
BspTI	CTTAAG	1 cut(s)	2260
BspTNI	GGTCTC	1 cut(s)	1998
BsrDI	GCAATG	1 cut(s)	2160
BsrFI	RCCGGY	1 cut(s)	443
BsrI	ACTGG	8 cut(s)	109, 165, 233, 461, 856, 895, 1848, 2477
BssAI	RCCGGY	1 cut(s)	443
BssECI	CCNNGG	8 cut(s)	22, 176, 494, 989, 1166, 1254, 1691, 2433
BssNAI	GTATAC	1 cut(s)	2375
BssNI	GRCGYC	1 cut(s)	1269
BssSI	CACGAG	2 cut(s)	1799, 1953
BssT1I	CCWWGG	1 cut(s)	989
Bst1107I	GTATAC	1 cut(s)	2375
Bst2BI	CACGAG	2 cut(s)	1799, 1953
Bst2UI	CCWGG	4 cut(s)	24, 1167, 1692, 2546
Bst4CI	ACNGT	4 cut(s)	1354, 1672, 2132, 2391
Bst6I	CTCTTC	1 cut(s)	840
BstACI	GRCGYC	1 cut(s)	1269
BstAFI	CTTAAG	1 cut(s)	2260
BstBI	TTCGAA	1 cut(s)	156
BstC8I	GCNNGC	4 cut(s)	445, 659, 2017, 2243
BstDEI	CTNAG	8 cut(s)	139, 401, 728, 833, 876, 1150, 1779, 1988
BstDSI	CCRYGG	1 cut(s)	2433
BstF5I	GGATG	8 cut(s)	328, 472, 504, 941, 1014, 1401, 1891, 2038
BstHHI	GCGC	1 cut(s)	1318
BstMAI	GTCTC	2 cut(s)	1998, 2500
BstMCI	CGRYCG	3 cut(s)	393, 576, 2433
BstMWI	GCNNNNNNNGC	4 cut(s)	362, 444, 658, 697
BstNI	CCWGG	4 cut(s)	24, 1167, 1692, 2546
BstNSI	RCATGY	4 cut(s)	1861, 2343, 2381, 2513
BstSCI	CCNGG	7 cut(s)	22, 175, 493, 1165, 1253, 1690, 2544
BstX2I	RGATCY	1 cut(s)	1577
BstXI	CCANNNNNNTGG	1 cut(s)	240
BstYI	RGATCY	1 cut(s)	1577
BstZ17I	GTATAC	1 cut(s)	2375
BstZI	CGGCCG	2 cut(s)	573, 2430
BsuI	GTATCC	1 cut(s)	2515
BsuRI	GGCC	5 cut(s)	108, 575, 2226, 2432, 2544
BtgI	CCRYGG	1 cut(s)	2433
BtgZI	GCGATG	3 cut(s)	315, 453, 2314
BtsCI	GGATG	8 cut(s)	328, 472, 504, 941, 1014, 1401, 1891, 2038
BtsI	GCAGTG	2 cut(s)	1061, 1486
BtsIMutI	CAGTG	6 cut(s)	240, 409, 863, 902, 1061, 1486
BveI	ACCTGC	1 cut(s)	1151
Cac8I	GCNNGC	4 cut(s)	445, 659, 2017, 2243
CfoI	GCGC	1 cut(s)	1318
Cfr10I	RCCGGY	1 cut(s)	443
Cfr13I	GGNCC	4 cut(s)	106, 1163, 1251, 2311
CseI	GACGC	1 cut(s)	75
Csp6I	GTAC	7 cut(s)	196, 780, 820, 1071, 1209, 1668, 2535
CspCI	CAANNNNNGTGG	2 cut(s)	898, 933
CviQI	GTAC	7 cut(s)	196, 780, 820, 1071, 1209, 1668, 2535
DdeI	CTNAG	8 cut(s)	139, 401, 728, 833, 876, 1150, 1779, 1988
DraIII	CACNNNGTG	1 cut(s)	404
EaeI	YGGCCR	3 cut(s)	573, 2224, 2430
EagI	CGGCCG	2 cut(s)	573, 2430
Eam1104I	CTCTTC	1 cut(s)	840
EarI	CTCTTC	1 cut(s)	840
Ecl136II	GAGCTC	1 cut(s)	753
EclXI	CGGCCG	2 cut(s)	573, 2430
Eco130I	CCWWGG	1 cut(s)	989
Eco24I	GRGCYC	2 cut(s)	755, 1962
Eco31I	GGTCTC	1 cut(s)	1998
Eco47I	GGWCC	3 cut(s)	1163, 1251, 2311
Eco52I	CGGCCG	2 cut(s)	573, 2430
Eco53kI	GAGCTC	1 cut(s)	753
Eco57I	CTGAAG	4 cut(s)	1518, 1656, 2379, 2453
Eco88I	CYCGRG	2 cut(s)	603, 2112
EcoICRI	GAGCTC	1 cut(s)	753
EcoRI	GAATTC	3 cut(s)	181, 204, 1517
EcoRII	CCWGG	4 cut(s)	22, 1165, 1690, 2544
EcoT14I	CCWWGG	1 cut(s)	989
EcoT22I	ATGCAT	3 cut(s)	978, 1222, 2281
EcoT38I	GRGCYC	2 cut(s)	755, 1962
ErhI	CCWWGG	1 cut(s)	989
FalI	AAGNNNNNCTT	4 cut(s)	402, 434, 553, 585
FaqI	GGGAC	3 cut(s)	827, 1007, 1271
FauI	CCCGC	3 cut(s)	237, 650, 1372
FbaI	TGATCA	1 cut(s)	469
FblI	GTMKAC	6 cut(s)	347, 453, 1674, 1733, 2374, 2416
FokI	GGATG	8 cut(s)	335, 459, 511, 928, 1021, 1388, 1878, 2045
FriOI	GRGCYC	2 cut(s)	755, 1962
FspBI	CTAG	6 cut(s)	990, 1913, 2016, 2210, 2238, 2412
FspI	TGCGCA	1 cut(s)	1317
GlaI	GCGC	1 cut(s)	1317
GsuI	CTGGAG	1 cut(s)	300
HaeIII	GGCC	5 cut(s)	108, 575, 2226, 2432, 2544
HapII	CCGG	7 cut(s)	176, 193, 429, 444, 494, 576, 1254
HgaI	GACGC	1 cut(s)	75
HhaI	GCGC	1 cut(s)	1318
Hin1I	GRCGYC	1 cut(s)	1269
Hin6I	GCGC	1 cut(s)	1316
HinP1I	GCGC	1 cut(s)	1316
HincII	GTYRAC	2 cut(s)	1156, 1546
HindII	GTYRAC	2 cut(s)	1156, 1546
HindIII	AAGCTT	2 cut(s)	1646, 1870
HpaI	GTTAAC	2 cut(s)	1156, 1546
HpaII	CCGG	7 cut(s)	176, 193, 429, 444, 494, 576, 1254
HphI	GGTGA	3 cut(s)	284, 523, 2133
Hpy166II	GTNNAC	9 cut(s)	348, 454, 1156, 1251, 1546, 1675, 1734, 2375, 2417
Hpy188III	TCNNGA	9 cut(s)	30, 279, 1536, 1725, 1756, 1801, 1976, 1983, 2003
Hpy8I	GTNNAC	9 cut(s)	348, 454, 1156, 1251, 1546, 1675, 1734, 2375, 2417
Hpy99I	CGWCG	3 cut(s)	91, 203, 1274
HpyCH4III	ACNGT	4 cut(s)	1354, 1672, 2132, 2391
HpyCH4IV	ACGT	4 cut(s)	198, 1269, 1298, 1548
HpyF10VI	GCNNNNNNNGC	4 cut(s)	362, 444, 658, 697
HpyF3I	CTNAG	8 cut(s)	139, 401, 728, 833, 876, 1150, 1779, 1988
HpySE526I	ACGT	4 cut(s)	198, 1269, 1298, 1548
Hsp92I	GRCGYC	1 cut(s)	1269
HspAI	GCGC	1 cut(s)	1316
KroI	GCCGGC	1 cut(s)	443
KroNI	GCCGGC	1 cut(s)	445
Ksp22I	TGATCA	1 cut(s)	469
KspAI	GTTAAC	2 cut(s)	1156, 1546
LmnI	GCTCC	4 cut(s)	750, 826, 1382, 1965
LweI	GCATC	5 cut(s)	430, 508, 950, 1489, 2293
MaeI	CTAG	6 cut(s)	990, 1913, 2016, 2210, 2238, 2412
MaeII	ACGT	4 cut(s)	198, 1269, 1298, 1548
MaeIII	GTNAC	8 cut(s)	404, 563, 581, 1331, 1782, 2139, 2385, 2569
MflI	RGATCY	1 cut(s)	1577
MhlI	GDGCHC	3 cut(s)	755, 1387, 1962
MlyI	GAGTC	1 cut(s)	989
MmeI	TCCRAC	4 cut(s)	181, 537, 1005, 1725
Mph1103I	ATGCAT	3 cut(s)	978, 1222, 2281
MroNI	GCCGGC	1 cut(s)	443
MseI	TTAA	6 cut(s)	1155, 1472, 1545, 2261, 2441, 2502
MslI	CAYNNNNRTG	2 cut(s)	686, 1355
MspA1I	CMGCKG	4 cut(s)	435, 479, 1591, 1616
MspCI	CTTAAG	1 cut(s)	2260
MspI	CCGG	7 cut(s)	176, 193, 429, 444, 494, 576, 1254
MspR9I	CCNGG	7 cut(s)	24, 177, 495, 1167, 1255, 1692, 2546
Mva1269I	GAATGC	3 cut(s)	693, 745, 1318
MvaI	CCWGG	4 cut(s)	24, 1167, 1692, 2546
MwoI	GCNNNNNNNGC	4 cut(s)	362, 444, 658, 697
NaeI	GCCGGC	1 cut(s)	445
NciI	CCSGG	3 cut(s)	177, 495, 1255
NgoMIV	GCCGGC	1 cut(s)	443
NheI	GCTAGC	1 cut(s)	2015
NlaIV	GGNNCC	6 cut(s)	28, 334, 1126, 1579, 1961, 2125
NmuCI	GTSAC	3 cut(s)	404, 563, 2139
NsbI	TGCGCA	1 cut(s)	1317
NsiI	ATGCAT	3 cut(s)	978, 1222, 2281
NspI	RCATGY	4 cut(s)	1861, 2343, 2381, 2513
NspV	TTCGAA	1 cut(s)	156
OliI	CACNNNNGTG	1 cut(s)	1355
PaeR7I	CTCGAG	1 cut(s)	603
PciI	ACATGT	3 cut(s)	2339, 2377, 2509
PcsI	WCGNNNNNNNCGW	1 cut(s)	649
PctI	GAATGC	3 cut(s)	693, 745, 1318
PdiI	GCCGGC	1 cut(s)	445
PflMI	CCANNNNNTGG	1 cut(s)	269
PleI	GAGTC	1 cut(s)	989
PpsI	GAGTC	1 cut(s)	989
PscI	ACATGT	3 cut(s)	2339, 2377, 2509
Psp124BI	GAGCTC	1 cut(s)	755
Psp1406I	AACGTT	1 cut(s)	1548
Psp6I	CCWGG	4 cut(s)	22, 1165, 1690, 2544
PspGI	CCWGG	4 cut(s)	22, 1165, 1690, 2544
PspN4I	GGNNCC	6 cut(s)	28, 334, 1126, 1579, 1961, 2125
PspPI	GGNCC	4 cut(s)	106, 1163, 1251, 2311
PspXI	VCTCGAGB	1 cut(s)	603
PsuI	RGATCY	1 cut(s)	1577
PvuII	CAGCTG	1 cut(s)	1616
RsaI	GTAC	7 cut(s)	197, 781, 821, 1072, 1210, 1669, 2536
RsaNI	GTAC	7 cut(s)	196, 780, 820, 1071, 1209, 1668, 2535
RseI	CAYNNNNRTG	2 cut(s)	686, 1355
SacI	GAGCTC	1 cut(s)	755
SaqAI	TTAA	6 cut(s)	1155, 1472, 1545, 2261, 2441, 2502
Sau96I	GGNCC	4 cut(s)	106, 1163, 1251, 2311
ScaI	AGTACT	2 cut(s)	781, 1072
SchI	GAGTC	1 cut(s)	989
ScrFI	CCNGG	7 cut(s)	24, 177, 495, 1167, 1255, 1692, 2546
SduI	GDGCHC	3 cut(s)	755, 1387, 1962
SfaNI	GCATC	5 cut(s)	430, 508, 950, 1489, 2293
Sfr274I	CTCGAG	1 cut(s)	603
SfuI	TTCGAA	1 cut(s)	156
SinI	GGWCC	3 cut(s)	1163, 1251, 2311
SlaI	CTCGAG	1 cut(s)	603
SmiMI	CAYNNNNRTG	2 cut(s)	686, 1355
SmlI	CTYRAG	3 cut(s)	603, 1737, 2260
SmoI	CTYRAG	3 cut(s)	603, 1737, 2260
SspI	AATATT	1 cut(s)	2079
SspMI	CTAG	6 cut(s)	990, 1913, 2016, 2210, 2238, 2412
SstI	GAGCTC	1 cut(s)	755
StyD4I	CCNGG	7 cut(s)	22, 175, 493, 1165, 1253, 1690, 2544
StyI	CCWWGG	1 cut(s)	989
TaaI	ACNGT	4 cut(s)	1354, 1672, 2132, 2391
TaiI	ACGT	4 cut(s)	201, 1272, 1301, 1551
TatI	WGTACW	4 cut(s)	779, 1070, 1208, 2534
TauI	GCSGC	3 cut(s)	438, 616, 1088
Tru1I	TTAA	6 cut(s)	1155, 1472, 1545, 2261, 2441, 2502
Tru9I	TTAA	6 cut(s)	1155, 1472, 1545, 2261, 2441, 2502
TscAI	CASTG	6 cut(s)	240, 409, 863, 902, 1061, 1486
TseFI	GTSAC	3 cut(s)	404, 563, 2139
Tsp45I	GTSAC	3 cut(s)	404, 563, 2139
TspGWI	ACGGA	3 cut(s)	161, 2267, 2324
TspRI	CASTG	6 cut(s)	240, 409, 863, 902, 1061, 1486
Van91I	CCANNNNNTGG	1 cut(s)	269
Vha464I	CTTAAG	1 cut(s)	2260
VpaK11BI	GGWCC	3 cut(s)	1163, 1251, 2311
XapI	RAATTY	8 cut(s)	33, 150, 181, 204, 1305, 1517, 2052, 2528
XceI	RCATGY	4 cut(s)	1861, 2343, 2381, 2513
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2431
XhoI	CTCGAG	1 cut(s)	603
XmaJI	CCTAGG	1 cut(s)	989
XmiI	GTMKAC	6 cut(s)	347, 453, 1674, 1733, 2374, 2416
XspI	CTAG	6 cut(s)	990, 1913, 2016, 2210, 2238, 2412
ZraI	GACGTC	1 cut(s)	1270
ZrmI	AGTACT	2 cut(s)	781, 1072
Zsp2I	ATGCAT	3 cut(s)	978, 1222, 2281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.