Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007917

Physical Location & Seq

Genomic Coordinates Forward (+)

39230195 .. 39233884

Gene Structure 3690 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1306600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGAATGCCTGCACTTCTGTGAGTTCATCAATGGGAGTCGATAGAAAAAACCCAGGTACCAGAATTTGTAACTTCTCATTCCTTATTGTTTTCTGCTGGGCTCTCCCTCTGTTTTGCTCTGCGTCGAGTTCGATTTCGCGGGGGCAATCTATAAAAGATGGAGAGACTCTGATCTCGGATGGCCAAATATTCGAACTGGGTTTCTTCAGTCTGGGGAATTCATCGTCCCGGTACGTCGGAATTCGATATTACAAAATTTCGGACCCGTCAGTGATCTGGGTTGCAAACAGAGAAAACCCAATTTCTGATAAATCTGGAGTTTTGGAATTTAGAAGCGATGGAAATCTGGTTGTTTTGGATGGAAATGGCGCCACTGTTTGGTCTACTAACGCTTCAGCCGCCACTAACTCGACGGCGAGGCTCAATGACGAAGGAAGTCTTATTCTTTCCCGCCGCGGCGATGCCAGCATGGTCTATTGGCAGAGCTTTGACGATGCAGCGGACACATTTTTGCCGGGAATGAAAGTTGAGGTGAGTGATGAGATCGGAGAGAATCGGTTTCTGAGTTCGTGGAAGTCGGAAAGTGACCCTTCGGCCGGAGGGTACTCAATGGGCGTTGATCCTCGAGGATCGCCGCAGATTGTGATATGGGAAGGATCAAAACGGCGGTGGAGAAGCGGGCATTGGAACAAGCAGATATTCATTGGACTTCCGAATATGCCAACTACTTATGCATATGGTTTTAAGCTTAGTGATGAGAATGGGAAATCATACTTTACTTACAAGCCGTGGAATCCTTCGGATAAGTTGAGGTTTCGGATAAGGTGGGACGGGTACGAAGAGCAGCTGAAGTGGGCGGAAGAGAAGAGCCAGTGGGATGTGATACAATCTCAGCCTAACAAGACCAACCAATGTGAGTTTTACAACAAATGTGGAAGGTTTGGGGTGTGTAGTGCATCTGATGAATCGAGTTCAATTTGCCGTTGTATGCGTGGATTTCAACCGAGGAATTTGGATCAATGGACTGGGGGAAATTGGTCGGAAGGGTGTTATAGGAAAACCCCTTTGCAGTGTCAGGGAAATAGTACTAATGGAACGGTGGCAAGTGATGAAAAAGATGGATTTGTAGCGATAAGGTGTGCCAAGTTGCCGGATTTTGCAGACTTCGTTGTTGCAGTTCCAGGGGAGAGCTGCGAGGAGAAATGTCTCGAGAATTGTGCTTGTACTGCATATGCAAATGTTCAAGGGATAGGTTGTATGATGTGGACCGAAGACTTGATTGATGTCGAACAATTTACAAAGGGAGGAAACACATTGCATATTCGTGTTGCGCATTCAGATTTAGTGTTGCCCACCACTTCTTCAATTTCATGGTTAAGGGGAGGTGATACATCAACGCTCAATGCTGGAAAAAGTACAGAATTTTCAACAGATGCTTCAGGATCGGTAGATGTTTATGCAGAAGGGAATCAAGTAAATAGATCCGAATTACGGCTGTTAAATTTGAGTTGTGTAGCAGCTGCAACGAACAACTTTTGTGAAGAAAATAAGCTTGGGAAGGGGGGATTTGGTACTGTCTACAAGGGTAGCCTTCCAGGGCTACCACAAATAGCTGTAAAAAGACTTTCGAGAAGGTCTACTCAAGGATTGGAGGAGTTCAAGAATGAGATTAGTCTGATTGCTAAGTTACAACACAGGAATCTCGTGAGACTATTGGGCTGCTGCATTGAAGGAGAAGAAAAGATGCTGGTTTATGAATACATGCCAAACAAAAGCTTGGATTTCTTTCTCTTCGATCCAGCCAACCAATCACTACTAGATTGGAGAAAACGCTTCACAATTATTGAAGGGATTGCACGAGGGCTACTTTATCTCCATCGTGATTCAAGACTTAGAATTATTCATCGAGACCTAAAAGCTAGCAACATTTTGCTGGATGAAGATATGATTCCCAAAATTTCAGACTTTGGTATGGCAAGAATATTCGGGGGTAATGAAAATGAAGAAAATACAGCCCGAGTTGTTGGCACATACGGTTATATGTCACCTGAATATGCAATGGAAGGTCTGTTTTCAGTGAAGTCAGATGTCTATAGCTTTGGTGTATTGCTGCTAGAGATTGTGAGTGGCCGAAGGAACACAAGTTTGCGATCAACGGAACACTTAAGCCTTATTGGATATGCATGGCATCTTTGGAACGAGAATCGAGCAGTGGACCTCATTGATCCTTCCATCGCAGAAACATGTTCCCAGAATGAAACTGAATTATTGAGATGCATACATGTAGGGTTGCTGTGTGTGCAAGACTACGCAGCTAGTAGACCAACCATGCCGGCCGTGGTGTTGATGCTGGAAAGTGAAGCTGCAAATCTTCAGTTACCAGTACAACCTATATTTACTTCAATTAGGAGACCCGTGGATACAAAATTTGGTACAGAAGGCCAGGATGTAGCATCCTCGAACAATGTAACGATTACAGTGTTGGAAGGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

832

Amino Acids

92.4

Weight (kDa)

5.48

Isoelectric Point (pI)

45.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	91 - 192	1.4e-32	D-mannose binding lectin
S_locus_glycop	PF00954	224 - 336	1.5e-26	S-locus glycoprotein domain
PAN_2	PF08276	370 - 429	1.4e-16	PAN-like domain
Pkinase	PF00069	514 - 715	1.2e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	515 - 723	7.3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007917)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61610 AT1G61610 AT4G21390 AT4G21390
fragaria_vesca	FvH4_3g03590 FvH4_3g03590 FvH4_3g03590 FvH4_3g03590 FvH4_3g03590
malus_domestica	MD05G1329600.v1.1 MD10G1306600.v1.1
prunus_persica	Prupe.4G033200_v2.0.a1
pyrus_communis	pycom05g30270 pycom10g25870
rosa_chinensis	RchiOBHm_Chr5g0005641
rosa_laevigata	RLG00000031314
rosa_multiflora	Rmu_co8421279.1_g000001
rosa_roxburghii	Rroxscaffold_1G00070190
rosa_rugosa	Rorug04G0417000
rosa_samantha	Rh5AG044400 Rh5BG043200 Rh5CG052000 Rh5DG043200
rosa_wichuraiana	Rw5G004470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	624
Acc16I	TGCGCA	1 cut(s)	1332
Acc65I	GGTACC	1 cut(s)	56
AccB1I	GGYRCC	2 cut(s)	56, 368
AccB7I	CCANNNNNTGG	1 cut(s)	378
AccI	GTMKAC	4 cut(s)	383, 1578, 1637, 2320
AccII	CGCG	2 cut(s)	139, 456
AclWI	GGATC	8 cut(s)	614, 637, 664, 1023, 1450, 1476, 1790, 2219
AcoI	YGGCCR	4 cut(s)	181, 594, 2128, 2334
AcuI	CTGAAG	5 cut(s)	190, 378, 869, 1422, 2357
AcyI	GRCGYC	1 cut(s)	369
AfiI	CCNNNNNNNGG	3 cut(s)	378, 596, 1491
AflII	CTTAAG	1 cut(s)	2164
AflIII	ACRYGT	2 cut(s)	2243, 2281
AjnI	CCWGG	4 cut(s)	52, 1180, 1594, 2442
AjuI	GAANNNNNNNTTGG	4 cut(s)	667, 699, 1536, 1568
AleI	CACNNNNGTG	1 cut(s)	17
Alw26I	GTCTC	5 cut(s)	158, 1211, 1702, 1902, 2404
AlwI	GGATC	8 cut(s)	614, 637, 664, 1023, 1450, 1476, 1790, 2219
Ama87I	CYCGRG	3 cut(s)	624, 1208, 2016
AoxI	GGCC	5 cut(s)	181, 594, 2128, 2334, 2440
Asp718I	GGTACC	1 cut(s)	56
AspLEI	GCGC	2 cut(s)	371, 1333
AspS9I	GGNCC	3 cut(s)	262, 1266, 2215
AsuC2I	CCSGG	2 cut(s)	229, 516
AsuHPI	GGTGA	3 cut(s)	544, 1397, 2037
AsuII	TTCGAA	1 cut(s)	192
AsuNHI	GCTAGC	1 cut(s)	1919
AvaI	CYCGRG	3 cut(s)	624, 1208, 2016
AvaII	GGWCC	3 cut(s)	262, 1266, 2215
BaeI	ACNNNNGTAYC	2 cut(s)	40, 73
BalI	TGGCCA	1 cut(s)	183
BanI	GGYRCC	2 cut(s)	56, 368
BanII	GRGCYC	1 cut(s)	103
BauI	CACGAG	2 cut(s)	1703, 1857
BbsI	GAAGAC	1 cut(s)	1278
BccI	CCATC	7 cut(s)	152, 173, 332, 353, 1112, 1884, 2240
BceAI	ACGGC	6 cut(s)	429, 680, 772, 966, 1508, 2321
BcgI	CGANNNNNNTGC	2 cut(s)	1199, 1233
BciT130I	CCWGG	4 cut(s)	54, 1182, 1596, 2444
BciVI	GTATCC	1 cut(s)	2413
BcnI	CCSGG	2 cut(s)	229, 516
BcoDI	GTCTC	5 cut(s)	158, 1211, 1702, 1902, 2404
BfaI	CTAG	4 cut(s)	1817, 1920, 2114, 2316
BfmI	CTRYAG	1 cut(s)	2092
BfoI	RGCGCY	1 cut(s)	372
BfrI	CTTAAG	1 cut(s)	2164
BfuI	GTATCC	1 cut(s)	2413
BmcAI	AGTACT	1 cut(s)	1087
Bme1390I	CCNGG	6 cut(s)	54, 229, 516, 1182, 1596, 2444
Bme18I	GGWCC	3 cut(s)	262, 1266, 2215
BmeT110I	CYCGRG	3 cut(s)	624, 1208, 2016
BmgT120I	GGNCC	3 cut(s)	262, 1266, 2215
BmiI	GGNNCC	3 cut(s)	58, 264, 370
BmrFI	CCNGG	6 cut(s)	54, 229, 516, 1182, 1596, 2444
BmrI	ACTGGG	2 cut(s)	206, 1035
BmsI	GCATC	9 cut(s)	451, 484, 965, 1423, 1734, 2197, 2264, 2337, 2462
BmtI	GCTAGC	1 cut(s)	1923
BmuI	ACTGGG	2 cut(s)	206, 1035
BpiI	GAAGAC	1 cut(s)	1278
BpmI	CTGGAG	1 cut(s)	336
Bpu14I	TTCGAA	1 cut(s)	192
BpuEI	CTTGAG	1 cut(s)	1626
BpuMI	CCSGG	2 cut(s)	229, 516
BsaBI	GATNNNNATC	1 cut(s)	342
BsaHI	GRCGYC	1 cut(s)	369
BsaI	GGTCTC	2 cut(s)	1902, 2404
BsaJI	CCNNGG	8 cut(s)	52, 454, 788, 1004, 1181, 1595, 2337, 2415
BsaXI	ACNNNNNCTCC	2 cut(s)	1857, 1887
Bsc4I	CCNNNNNNNGG	3 cut(s)	378, 596, 1491
Bse118I	RCCGGY	1 cut(s)	2332
Bse1I	ACTGG	4 cut(s)	201, 871, 1030, 2381
Bse3DI	GCAATG	2 cut(s)	1313, 2064
Bse8I	GATNNNNATC	1 cut(s)	342
BseBI	CCWGG	4 cut(s)	54, 1182, 1596, 2444
BseDI	CCNNGG	8 cut(s)	52, 454, 788, 1004, 1181, 1595, 2337, 2415
BseGI	GGATG	6 cut(s)	184, 364, 883, 1942, 2452, 2453
BseJI	GATNNNNATC	1 cut(s)	342
BseLI	CCNNNNNNNGG	3 cut(s)	378, 596, 1491
BseMI	GCAATG	2 cut(s)	1313, 2064
BseMII	CTCAG	2 cut(s)	554, 905
BseNI	ACTGG	4 cut(s)	201, 871, 1030, 2381
BseRI	GAGGAG	2 cut(s)	1211, 1667
BseX3I	CGGCCG	2 cut(s)	594, 2334
BseYI	CCCAGC	1 cut(s)	96
Bsh1236I	CGCG	2 cut(s)	139, 456
Bsh1285I	CGRYCG	2 cut(s)	597, 2337
BshFI	GGCC	5 cut(s)	183, 596, 2130, 2336, 2442
BshNI	GGYRCC	2 cut(s)	56, 368
BsiEI	CGRYCG	2 cut(s)	597, 2337
BsiHKCI	CYCGRG	3 cut(s)	624, 1208, 2016
BsiSI	CCGG	5 cut(s)	229, 515, 597, 1151, 2333
BslFI	GGGAC	2 cut(s)	211, 842
BslI	CCNNNNNNNGG	3 cut(s)	378, 596, 1491
BsmAI	GTCTC	5 cut(s)	158, 1211, 1702, 1902, 2404
BsmFI	GGGAC	2 cut(s)	211, 842
BsmI	GAATGC	2 cut(s)	10, 1333
BsnI	GGCC	5 cut(s)	183, 596, 2130, 2336, 2442
Bso31I	GGTCTC	2 cut(s)	1902, 2404
BsoBI	CYCGRG	3 cut(s)	624, 1208, 2016
Bsp119I	TTCGAA	1 cut(s)	192
Bsp1286I	GDGCHC	1 cut(s)	103
BspANI	GGCC	5 cut(s)	183, 596, 2130, 2336, 2442
BspCNI	CTCAG	2 cut(s)	555, 904
BspFNI	CGCG	2 cut(s)	139, 456
BspLI	GGNNCC	3 cut(s)	58, 264, 370
BspOI	GCTAGC	1 cut(s)	1923
BspPI	GGATC	8 cut(s)	614, 637, 664, 1023, 1450, 1476, 1790, 2219
BspQI	GCTCTTC	2 cut(s)	834, 860
BspT104I	TTCGAA	1 cut(s)	192
BspT107I	GGYRCC	2 cut(s)	56, 368
BspTI	CTTAAG	1 cut(s)	2164
BspTNI	GGTCTC	2 cut(s)	1902, 2404
BsrDI	GCAATG	2 cut(s)	1313, 2064
BsrFI	RCCGGY	1 cut(s)	2332
BsrI	ACTGG	4 cut(s)	201, 871, 1030, 2381
BssAI	RCCGGY	1 cut(s)	2332
BssECI	CCNNGG	8 cut(s)	52, 454, 788, 1004, 1181, 1595, 2337, 2415
BssNI	GRCGYC	1 cut(s)	369
BssSI	CACGAG	2 cut(s)	1703, 1857
Bst2BI	CACGAG	2 cut(s)	1703, 1857
Bst2UI	CCWGG	4 cut(s)	54, 1182, 1596, 2444
Bst4CI	ACNGT	5 cut(s)	376, 1099, 1576, 2036, 2479
Bst6I	CTCTTC	4 cut(s)	834, 855, 860, 1796
BstACI	GRCGYC	1 cut(s)	369
BstAFI	CTTAAG	1 cut(s)	2164
BstBI	TTCGAA	1 cut(s)	192
BstC8I	GCNNGC	5 cut(s)	10, 466, 680, 1921, 2334
BstDEI	CTNAG	5 cut(s)	563, 749, 891, 1683, 1892
BstDSI	CCRYGG	4 cut(s)	454, 788, 2337, 2415
BstF5I	GGATG	6 cut(s)	184, 364, 883, 1942, 2452, 2453
BstFNI	CGCG	2 cut(s)	139, 456
BstH2I	RGCGCY	1 cut(s)	372
BstHHI	GCGC	2 cut(s)	371, 1333
BstMAI	GTCTC	5 cut(s)	158, 1211, 1702, 1902, 2404
BstMCI	CGRYCG	2 cut(s)	597, 2337
BstMWI	GCNNNNNNNGC	4 cut(s)	398, 465, 1226, 2299
BstNI	CCWGG	4 cut(s)	54, 1182, 1596, 2444
BstNSI	RCATGY	3 cut(s)	1765, 2247, 2285
BstSCI	CCNGG	6 cut(s)	52, 227, 514, 1180, 1594, 2442
BstSFI	CTRYAG	1 cut(s)	2092
BstUI	CGCG	2 cut(s)	139, 456
BstV2I	GAAGAC	1 cut(s)	1278
BstX2I	RGATCY	1 cut(s)	1481
BstYI	RGATCY	1 cut(s)	1481
BstZI	CGGCCG	2 cut(s)	594, 2334
BsuI	GTATCC	1 cut(s)	2413
BsuRI	GGCC	5 cut(s)	183, 596, 2130, 2336, 2442
BtgI	CCRYGG	4 cut(s)	454, 788, 2337, 2415
BtgZI	GCGATG	3 cut(s)	351, 474, 2218
BtsCI	GGATG	6 cut(s)	184, 364, 883, 1942, 2452, 2453
BtsI	GCAGTG	2 cut(s)	1076, 2217
BtsIMutI	CAGTG	7 cut(s)	276, 372, 878, 1076, 2082, 2217, 2484
Cac8I	GCNNGC	5 cut(s)	10, 466, 680, 1921, 2334
CfoI	GCGC	2 cut(s)	371, 1333
Cfr10I	RCCGGY	1 cut(s)	2332
Cfr13I	GGNCC	3 cut(s)	262, 1266, 2215
Cfr42I	CCGCGG	1 cut(s)	457
CseI	GACGC	1 cut(s)	111
CspCI	CAANNNNNGTGG	2 cut(s)	913, 948
CviAII	CATG	7 cut(s)	469, 1371, 1762, 2184, 2244, 2282, 2329
DdeI	CTNAG	5 cut(s)	563, 749, 891, 1683, 1892
DinI	GGCGCC	1 cut(s)	370
EaeI	YGGCCR	4 cut(s)	181, 594, 2128, 2334
EagI	CGGCCG	2 cut(s)	594, 2334
Eam1104I	CTCTTC	4 cut(s)	834, 855, 860, 1796
EarI	CTCTTC	4 cut(s)	834, 855, 860, 1796
EciI	GGCGGA	1 cut(s)	872
EclXI	CGGCCG	2 cut(s)	594, 2334
Eco24I	GRGCYC	1 cut(s)	103
Eco31I	GGTCTC	2 cut(s)	1902, 2404
Eco47I	GGWCC	3 cut(s)	262, 1266, 2215
Eco52I	CGGCCG	2 cut(s)	594, 2334
Eco57I	CTGAAG	5 cut(s)	190, 378, 869, 1422, 2357
Eco88I	CYCGRG	3 cut(s)	624, 1208, 2016
EcoRI	GAATTC	2 cut(s)	217, 240
EcoRII	CCWGG	4 cut(s)	52, 1180, 1594, 2442
EcoT22I	ATGCAT	3 cut(s)	736, 2185, 2279
EcoT38I	GRGCYC	1 cut(s)	103
EgeI	GGCGCC	1 cut(s)	370
EheI	GGCGCC	1 cut(s)	370
FaeI	CATG	7 cut(s)	472, 1374, 1765, 2187, 2247, 2285, 2332
FalI	AAGNNNNNCTT	4 cut(s)	423, 455, 574, 606
FaqI	GGGAC	2 cut(s)	211, 842
FatI	CATG	7 cut(s)	468, 1370, 1761, 2183, 2243, 2281, 2328
FauI	CCCGC	3 cut(s)	132, 458, 671
FauNDI	CATATG	2 cut(s)	736, 1231
FblI	GTMKAC	4 cut(s)	383, 1578, 1637, 2320
FokI	GGATG	6 cut(s)	191, 371, 890, 1949, 2440, 2459
FriOI	GRGCYC	1 cut(s)	103
FspBI	CTAG	4 cut(s)	1817, 1920, 2114, 2316
FspI	TGCGCA	1 cut(s)	1332
GlaI	GCGC	2 cut(s)	370, 1332
GsaI	CCCAGC	1 cut(s)	100
GsuI	CTGGAG	1 cut(s)	336
HaeII	RGCGCY	1 cut(s)	372
HaeIII	GGCC	5 cut(s)	183, 596, 2130, 2336, 2442
HapII	CCGG	5 cut(s)	229, 515, 597, 1151, 2333
HgaI	GACGC	1 cut(s)	111
HhaI	GCGC	2 cut(s)	371, 1333
Hin1I	GRCGYC	1 cut(s)	369
Hin1II	CATG	7 cut(s)	472, 1374, 1765, 2187, 2247, 2285, 2332
Hin6I	GCGC	2 cut(s)	369, 1331
HinP1I	GCGC	2 cut(s)	369, 1331
HindIII	AAGCTT	3 cut(s)	746, 1550, 1774
HpaII	CCGG	5 cut(s)	229, 515, 597, 1151, 2333
HphI	GGTGA	3 cut(s)	544, 1397, 2037
Hpy166II	GTNNAC	6 cut(s)	384, 1266, 1579, 1638, 2215, 2321
Hpy8I	GTNNAC	6 cut(s)	384, 1266, 1579, 1638, 2215, 2321
Hpy99I	CGWCG	3 cut(s)	127, 239, 415
HpyCH4III	ACNGT	5 cut(s)	376, 1099, 1576, 2036, 2479
HpyCH4IV	ACGT	1 cut(s)	234
HpyF10VI	GCNNNNNNNGC	4 cut(s)	398, 465, 1226, 2299
HpyF3I	CTNAG	5 cut(s)	563, 749, 891, 1683, 1892
HpySE526I	ACGT	1 cut(s)	234
Hsp92I	GRCGYC	1 cut(s)	369
Hsp92II	CATG	7 cut(s)	472, 1374, 1765, 2187, 2247, 2285, 2332
HspAI	GCGC	2 cut(s)	369, 1331
KasI	GGCGCC	1 cut(s)	368
KpnI	GGTACC	1 cut(s)	60
KroI	GCCGGC	1 cut(s)	2332
KroNI	GCCGGC	1 cut(s)	2334
KspI	CCGCGG	1 cut(s)	457
LguI	GCTCTTC	2 cut(s)	834, 860
LweI	GCATC	9 cut(s)	451, 484, 965, 1423, 1734, 2197, 2264, 2337, 2462
MaeI	CTAG	4 cut(s)	1817, 1920, 2114, 2316
MaeII	ACGT	1 cut(s)	234
MaeIII	GTNAC	6 cut(s)	68, 584, 1686, 2043, 2376, 2467
MflI	RGATCY	1 cut(s)	1481
MhlI	GDGCHC	1 cut(s)	103
MlsI	TGGCCA	1 cut(s)	183
MluNI	TGGCCA	1 cut(s)	183
Mly113I	GGCGCC	1 cut(s)	369
MlyI	GAGTC	2 cut(s)	45, 160
MmeI	TCCRAC	4 cut(s)	217, 558, 1020, 2463
Mox20I	TGGCCA	1 cut(s)	183
Mph1103I	ATGCAT	3 cut(s)	736, 2185, 2279
MroNI	GCCGGC	1 cut(s)	2332
MscI	TGGCCA	1 cut(s)	183
MseI	TTAA	4 cut(s)	744, 1376, 1499, 2165
MslI	CAYNNNNRTG	2 cut(s)	17, 1323
Msp20I	TGGCCA	1 cut(s)	183
MspA1I	CMGCKG	4 cut(s)	456, 500, 847, 1520
MspCI	CTTAAG	1 cut(s)	2164
MspI	CCGG	5 cut(s)	229, 515, 597, 1151, 2333
MspR9I	CCNGG	6 cut(s)	54, 229, 516, 1182, 1596, 2444
Mva1269I	GAATGC	2 cut(s)	10, 1333
MvaI	CCWGG	4 cut(s)	54, 1182, 1596, 2444
MvnI	CGCG	2 cut(s)	139, 456
MwoI	GCNNNNNNNGC	4 cut(s)	398, 465, 1226, 2299
NaeI	GCCGGC	1 cut(s)	2334
NarI	GGCGCC	1 cut(s)	369
NciI	CCSGG	2 cut(s)	229, 516
NdeI	CATATG	2 cut(s)	736, 1231
NgoMIV	GCCGGC	1 cut(s)	2332
NheI	GCTAGC	1 cut(s)	1919
NlaIII	CATG	7 cut(s)	472, 1374, 1765, 2187, 2247, 2285, 2332
NlaIV	GGNNCC	3 cut(s)	58, 264, 370
NmuCI	GTSAC	2 cut(s)	584, 2043
NsbI	TGCGCA	1 cut(s)	1332
NsiI	ATGCAT	3 cut(s)	736, 2185, 2279
NspI	RCATGY	3 cut(s)	1765, 2247, 2285
NspV	TTCGAA	1 cut(s)	192
OliI	CACNNNNGTG	1 cut(s)	17
PaeR7I	CTCGAG	2 cut(s)	624, 1208
PciI	ACATGT	2 cut(s)	2243, 2281
PciSI	GCTCTTC	2 cut(s)	834, 860
PctI	GAATGC	2 cut(s)	10, 1333
PdiI	GCCGGC	1 cut(s)	2334
PfeI	GAWTC	8 cut(s)	553, 793, 965, 1468, 1699, 1883, 1948, 2203
PflMI	CCANNNNNTGG	1 cut(s)	378
PleI	GAGTC	2 cut(s)	44, 160
PluTI	GGCGCC	1 cut(s)	372
PpsI	GAGTC	2 cut(s)	44, 160
PscI	ACATGT	2 cut(s)	2243, 2281
Psp6I	CCWGG	4 cut(s)	52, 1180, 1594, 2442
PspFI	CCCAGC	1 cut(s)	96
PspGI	CCWGG	4 cut(s)	52, 1180, 1594, 2442
PspN4I	GGNNCC	3 cut(s)	58, 264, 370
PspPI	GGNCC	3 cut(s)	262, 1266, 2215
PspXI	VCTCGAGB	1 cut(s)	624
PsuI	RGATCY	1 cut(s)	1481
PvuII	CAGCTG	2 cut(s)	847, 1520
RseI	CAYNNNNRTG	2 cut(s)	17, 1323
SacII	CCGCGG	1 cut(s)	457
SapI	GCTCTTC	2 cut(s)	834, 860
SaqAI	TTAA	4 cut(s)	744, 1376, 1499, 2165
Sau96I	GGNCC	3 cut(s)	262, 1266, 2215
ScaI	AGTACT	1 cut(s)	1087
SchI	GAGTC	2 cut(s)	45, 160
ScrFI	CCNGG	6 cut(s)	54, 229, 516, 1182, 1596, 2444
SduI	GDGCHC	1 cut(s)	103
SfaNI	GCATC	9 cut(s)	451, 484, 965, 1423, 1734, 2197, 2264, 2337, 2462
SfcI	CTRYAG	1 cut(s)	2092
SfoI	GGCGCC	1 cut(s)	370
Sfr274I	CTCGAG	2 cut(s)	624, 1208
Sfr303I	CCGCGG	1 cut(s)	457
SfuI	TTCGAA	1 cut(s)	192
SgrBI	CCGCGG	1 cut(s)	457
SinI	GGWCC	3 cut(s)	262, 1266, 2215
SlaI	CTCGAG	2 cut(s)	624, 1208
SmiMI	CAYNNNNRTG	2 cut(s)	17, 1323
SmlI	CTYRAG	4 cut(s)	624, 1208, 1641, 2164
SmoI	CTYRAG	4 cut(s)	624, 1208, 1641, 2164
SspDI	GGCGCC	1 cut(s)	368
SspI	AATATT	2 cut(s)	189, 1983
SspMI	CTAG	4 cut(s)	1817, 1920, 2114, 2316
StyD4I	CCNGG	6 cut(s)	52, 227, 514, 1180, 1594, 2442
TaaI	ACNGT	5 cut(s)	376, 1099, 1576, 2036, 2479
TaiI	ACGT	1 cut(s)	237
TaqII	GACCGA	1 cut(s)	1283
TatI	WGTACW	4 cut(s)	1085, 1223, 1415, 2383
TauI	GCSGC	4 cut(s)	401, 456, 459, 637
TfiI	GAWTC	8 cut(s)	553, 793, 965, 1468, 1699, 1883, 1948, 2203
Tru1I	TTAA	4 cut(s)	744, 1376, 1499, 2165
Tru9I	TTAA	4 cut(s)	744, 1376, 1499, 2165
TscAI	CASTG	7 cut(s)	276, 379, 878, 1076, 2082, 2217, 2484
TseFI	GTSAC	2 cut(s)	584, 2043
Tsp45I	GTSAC	2 cut(s)	584, 2043
TspGWI	ACGGA	1 cut(s)	2171
TspRI	CASTG	7 cut(s)	276, 379, 878, 1076, 2082, 2217, 2484
Van91I	CCANNNNNTGG	1 cut(s)	378
Vha464I	CTTAAG	1 cut(s)	2164
VpaK11BI	GGWCC	3 cut(s)	262, 1266, 2215
XceI	RCATGY	3 cut(s)	1765, 2247, 2285
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2335
XhoI	CTCGAG	2 cut(s)	624, 1208
XmiI	GTMKAC	4 cut(s)	383, 1578, 1637, 2320
XspI	CTAG	4 cut(s)	1817, 1920, 2114, 2316
ZrmI	AGTACT	1 cut(s)	1087
Zsp2I	ATGCAT	3 cut(s)	736, 2185, 2279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.