MD05G1334600.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010974
Physical Location & Seq
Forward (+)
45769490 .. 45771473
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1334600.v1.1.491

Sequence Viewer

Length: 486 bp
ATGTCTCTGACACCAATATCTTTGCTCACTCTCTATCTCCTTCTCTTCCTTCTTCCTCCCACCGCCACCTCAACCCTCCACAACCTCCTCCAAAGCCAAGGCCTCCCCGCCGGGCTCTTCCCGGACAATGTGAAGTCATACAATCTCGACCAGACGGGCCGCCTGGAGGTGCATCTGGACCGCTCGTGCATGACCAAGTTTGAGACCAGAGTCTACTTTGAAAGCGTAGTCAGAGCTAATCTCAGCTATGGAGTGCTCAGAGGCTTGGAGGGTTTGACTCAGGAGGAGCTTTTTCTTTGGCTGCCGGTGAAGGGAATCTCAGTGAGCGATCCGTCTTCTGGGCTGATTTTGTTTGACATTGGTGTTGCCCATAAGCAGCTTTCTCTCTCTCTCTTTGAAGACCCCCCGGTCTGCAAGCCTCAATCTCAAGGCATTTCACTGAAGAGTGAAGGAGGAAGGAAGATTGGATTTCAGGCCCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

162

Amino Acids

17.76

Weight (kDa)

6.82

Isoelectric Point (pI)

53.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 27 - 133 4.8e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61667 AT1G61667
fragaria_vesca FvH4_3g03180
malus_domestica MD05G1334600.v1.1 MD10G1309500.v1.1
prunus_persica Prupe.4G030700_v2.0.a1
pyrus_communis pycom05g30610 pycom10g26090
rosa_chinensis RchiOBHm_Chr5g0004811
rosa_laevigata RLG00000031262
rosa_multiflora Rmu_co7972276.1_g000001 Rmu_sc0008308.1_g000015 Rmu_sc0010871.1_g000007
rosa_roxburghii Rroxscaffold_1G00070800
rosa_rugosa Rorug04G0414000
rosa_samantha Rh5AG041800 Rh5BG041500 Rh5CG045400 Rh5DG041900
rosa_wichuraiana Rw5G004050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 213
AciI CCGC 4 cut(s) 63, 108, 160, 181
AclWI GGATC 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 461
AfiI CCNNNNNNNGG 3 cut(s) 166, 311, 338
AgsI TTSAA 2 cut(s) 221, 398
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 4 cut(s) 236, 246, 289, 379
AluI AGCT 4 cut(s) 236, 246, 289, 379
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 9, 197
AlwI GGATC 1 cut(s) 323
AoxI GGCC 3 cut(s) 100, 157, 474
ApeKI GCWGC 2 cut(s) 301, 376
AspS9I GGNCC 3 cut(s) 157, 178, 475
AsuC2I CCSGG 3 cut(s) 112, 122, 407
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 178
BanII GRGCYC 1 cut(s) 117
BauI CACGAG 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 327, 405
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 288, 388
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 3 cut(s) 112, 122, 407
BcoDI GTCTC 2 cut(s) 9, 197
BisI GCNGC 3 cut(s) 160, 302, 377
BlsI GCNGC 3 cut(s) 161, 303, 378
Bme1390I CCNGG 4 cut(s) 112, 122, 164, 407
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 3 cut(s) 157, 178, 475
BmrFI CCNGG 4 cut(s) 112, 122, 164, 407
BmsI GCATC 1 cut(s) 181
BoxI GACNNNNGTC 1 cut(s) 209
BpiI GAAGAC 2 cut(s) 327, 405
BplI GAGNNNNNCTC 2 cut(s) 225, 257
BpmI CTGGAG 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 411
BpuMI CCSGG 3 cut(s) 112, 122, 407
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 97, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 311, 338
Bse118I RCCGGY 1 cut(s) 304
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 97, 405
BseLI CCNNNNNNNGG 3 cut(s) 166, 311, 338
BseMII CTCAG 4 cut(s) 256, 271, 293, 333
BseRI GAGGAG 2 cut(s) 77, 299
BseXI GCAGC 2 cut(s) 288, 388
BshFI GGCC 3 cut(s) 102, 159, 476
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 4 cut(s) 111, 122, 305, 407
BslI CCNNNNNNNGG 3 cut(s) 166, 311, 338
BsmAI GTCTC 2 cut(s) 9, 197
BsnI GGCC 3 cut(s) 102, 159, 476
Bso31I GGTCTC 1 cut(s) 197
Bsp1286I GDGCHC 2 cut(s) 117, 258
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 4 cut(s) 63, 108, 160, 181
BspANI GGCC 3 cut(s) 102, 159, 476
BspCNI CTCAG 4 cut(s) 255, 270, 292, 332
BspPI GGATC 1 cut(s) 323
BspQI GCTCTTC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 97, 405
BssMI GATC 1 cut(s) 328
BssSI CACGAG 1 cut(s) 184
BssT1I CCWWGG 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 164
Bst6I CTCTTC 3 cut(s) 50, 122, 437
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 4 cut(s) 242, 257, 279, 319
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 2 cut(s) 9, 197
BstMBI GATC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 164
BstPAI GACNNNNGTC 1 cut(s) 209
BstSCI CCNGG 4 cut(s) 110, 120, 162, 405
BstV1I GCAGC 2 cut(s) 288, 388
BstV2I GAAGAC 2 cut(s) 327, 405
BsuRI GGCC 3 cut(s) 102, 159, 476
BtsIMutI CAGTG 2 cut(s) 327, 437
Cac8I GCNNGC 1 cut(s) 416
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 3 cut(s) 157, 178, 475
CviAII CATG 1 cut(s) 190
DdeI CTNAG 4 cut(s) 242, 257, 279, 319
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DrdI GACNNNNNNGTC 1 cut(s) 407
DseDI GACNNNNNNGTC 1 cut(s) 407
Eam1104I CTCTTC 3 cut(s) 50, 122, 437
EarI CTCTTC 3 cut(s) 50, 122, 437
Eco130I CCWWGG 1 cut(s) 97
Eco147I AGGCCT 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 1 cut(s) 193
FaiI YATR 4 cut(s) 139, 191, 249, 372
FatI CATG 1 cut(s) 189
FauI CCCGC 1 cut(s) 115
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 160, 302, 377
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 3 cut(s) 160, 302, 377
GluI GCNGC 3 cut(s) 160, 302, 377
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 3 cut(s) 102, 159, 476
HapII CCGG 4 cut(s) 111, 122, 305, 407
Hin1II CATG 1 cut(s) 193
HinfI GANTC 3 cut(s) 210, 277, 315
HpaII CCGG 4 cut(s) 111, 122, 305, 407
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 9, 233, 260
Hpy188III TCNNGA 3 cut(s) 146, 176, 281
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 5 cut(s) 50, 59, 304, 443, 450
HpyCH4V TGCA 3 cut(s) 172, 189, 414
HpyF3I CTNAG 4 cut(s) 242, 257, 279, 319
Hsp92II CATG 1 cut(s) 193
Kzo9I GATC 1 cut(s) 328
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 2 cut(s) 288, 388
LweI GCATC 1 cut(s) 181
MalI GATC 1 cut(s) 330
MbiI CCGCTC 1 cut(s) 183
MboI GATC 1 cut(s) 328
MboII GAAGA 7 cut(s) 37, 44, 109, 327, 410, 454, 472
MhlI GDGCHC 2 cut(s) 117, 258
MlyI GAGTC 2 cut(s) 219, 271
MspI CCGG 4 cut(s) 111, 122, 305, 407
MspR9I CCNGG 4 cut(s) 112, 122, 164, 407
MvaI CCWGG 1 cut(s) 164
NciI CCSGG 3 cut(s) 112, 122, 407
NdeII GATC 1 cut(s) 328
NlaIII CATG 1 cut(s) 193
PceI AGGCCT 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 315
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 3 cut(s) 161, 303, 378
PleI GAGTC 2 cut(s) 218, 271
PpsI GAGTC 2 cut(s) 218, 271
PshAI GACNNNNGTC 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspPI GGNCC 3 cut(s) 157, 178, 475
SapI GCTCTTC 1 cut(s) 122
SatI GCNGC 3 cut(s) 160, 302, 377
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 3 cut(s) 157, 178, 475
SchI GAGTC 2 cut(s) 219, 271
ScrFI CCNGG 4 cut(s) 112, 122, 164, 407
SduI GDGCHC 2 cut(s) 117, 258
SetI ASST 7 cut(s) 71, 87, 171, 238, 248, 291, 381
SfaNI GCATC 1 cut(s) 181
SinI GGWCC 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SseBI AGGCCT 1 cut(s) 102
SsiI CCGC 4 cut(s) 63, 108, 160, 181
StuI AGGCCT 1 cut(s) 102
StyD4I CCNGG 4 cut(s) 110, 120, 162, 405
StyI CCWWGG 1 cut(s) 97
TaqI TCGA 1 cut(s) 147
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 315
TscAI CASTG 2 cut(s) 327, 444
TseI GCWGC 2 cut(s) 301, 376
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 2 cut(s) 327, 444
VpaK11BI GGWCC 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.