Rw5G004050

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010974
Physical Location & Seq
Reverse (-)
4074715 .. 4076915
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004050.1

Sequence Viewer

Length: 483 bp
ATGTCTCTGACACCCGTATCTCTCTTCACTCTCTCTCTCCTCCTCCTCCTTCATCTTCCTTCCACAGTCACCTCATCTCTCCACAACCTCCTCCAGAGCCAAGGCCTCCCCGGCGGCCTCTTCCCCAACAACGTCAAGTCCTACAATCTCGACCCGATGGGCCGCCTCGAGGTCCACCTGGACTCTTCTTGCATGGCCAAGTTCGAGAATCGGGTCTACTTCGACAACACAGTCAGAGCCAATCTCAGCTTTGGTGTGCTTAAAGGCTTGGAGGGTCTGATTCAAGAAGAGCTGTTTCTGTGGTTACCAGTCAAAGGAATCTCAGTTAGTGATCCATCTTCTGGTTTGATTTTGTTTGATATTGGTGTTGCCCATAAGCAGCTTTCTCTTTCTCTCTTTGAAGACCCTCCTCTTTGTAACCCTCAAGGGGTTTCACTGAAGGAGGATGTGGTGAAGAACACTGGATTTGAGGCGCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

160

Amino Acids

17.52

Weight (kDa)

5.47

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 134 1.7e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61667 AT1G61667
fragaria_vesca FvH4_3g03180
malus_domestica MD05G1334600.v1.1 MD10G1309500.v1.1
prunus_persica Prupe.4G030700_v2.0.a1
pyrus_communis pycom05g30610 pycom10g26090
rosa_chinensis RchiOBHm_Chr5g0004811
rosa_laevigata RLG00000031262
rosa_multiflora Rmu_co7972276.1_g000001 Rmu_sc0008308.1_g000015 Rmu_sc0010871.1_g000007
rosa_roxburghii Rroxscaffold_1G00070800
rosa_rugosa Rorug04G0414000
rosa_samantha Rh5AG041800 Rh5BG041500 Rh5CG045400 Rh5DG041900
rosa_wichuraiana Rw5G004050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AbsI CCTCGAGG 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 341
AccI GTMKAC 1 cut(s) 216
AciI CCGC 2 cut(s) 114, 163
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 195
AcuI CTGAAG 1 cut(s) 458
AfiI CCNNNNNNNGG 4 cut(s) 169, 314, 341, 427
AgsI TTSAA 2 cut(s) 284, 401
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 3 cut(s) 249, 292, 382
AluI AGCT 3 cut(s) 249, 292, 382
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 4 cut(s) 103, 115, 160, 195
ApeKI GCWGC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 160, 172
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 61, 463
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 172
BaeI ACNNNNGTAYC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 391
BccI CCATC 2 cut(s) 151, 343
BciT130I CCWGG 1 cut(s) 179
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 9
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 3 cut(s) 115, 163, 380
BlsI GCNGC 3 cut(s) 116, 164, 381
Bme1390I CCNGG 2 cut(s) 111, 179
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 160, 172
BmrFI CCNGG 2 cut(s) 111, 179
BpiI GAAGAC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 408
BpuMI CCSGG 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 100, 109
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 314, 341, 427
Bse1I ACTGG 2 cut(s) 308, 466
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 100, 109
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 4 cut(s) 169, 314, 341, 427
BseMII CTCAG 2 cut(s) 259, 336
BseNI ACTGG 2 cut(s) 308, 466
BseRI GAGGAG 5 cut(s) 29, 32, 35, 80, 399
BseXI GCAGC 1 cut(s) 391
BshFI GGCC 4 cut(s) 105, 117, 162, 197
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 4 cut(s) 169, 314, 341, 427
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 4 cut(s) 105, 117, 162, 197
BsoBI CYCGRG 1 cut(s) 167
Bsp143I GATC 1 cut(s) 331
BspACI CCGC 2 cut(s) 114, 163
BspANI GGCC 4 cut(s) 105, 117, 162, 197
BspCNI CTCAG 2 cut(s) 258, 335
BspPI GGATC 1 cut(s) 326
BspQI GCTCTTC 1 cut(s) 282
BsrI ACTGG 2 cut(s) 308, 466
BssECI CCNNGG 2 cut(s) 100, 109
BssMI GATC 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 2 cut(s) 67, 232
Bst6I CTCTTC 4 cut(s) 29, 125, 190, 282
BstDEI CTNAG 2 cut(s) 245, 322
BstEII GGTNACC 1 cut(s) 303
BstF5I GGATG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 1 cut(s) 334
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNI CCWGG 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 109, 177
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 1 cut(s) 408
BsuRI GGCC 4 cut(s) 105, 117, 162, 197
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 434, 459
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 160, 172
CviAII CATG 1 cut(s) 193
DdeI CTNAG 2 cut(s) 245, 322
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 4 cut(s) 29, 125, 190, 282
EarI CTCTTC 4 cut(s) 29, 125, 190, 282
Eco130I CCWWGG 1 cut(s) 100
Eco147I AGGCCT 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 303
EcoO65I GGTNACC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 196
FaiI YATR 2 cut(s) 194, 375
FatI CATG 1 cut(s) 192
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 115, 163, 380
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 3 cut(s) 115, 163, 380
GlaI GCGC 1 cut(s) 474
GluI GCNGC 3 cut(s) 115, 163, 380
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 4 cut(s) 105, 117, 162, 197
HapII CCGG 1 cut(s) 111
HhaI GCGC 1 cut(s) 475
Hin1II CATG 1 cut(s) 196
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 4 cut(s) 182, 208, 280, 318
HpaII CCGG 1 cut(s) 111
HphI GGTGA 2 cut(s) 61, 463
Hpy166II GTNNAC 2 cut(s) 175, 217
Hpy188I TCNGA 3 cut(s) 9, 236, 279
Hpy188III TCNNGA 4 cut(s) 94, 149, 205, 284
Hpy8I GTNNAC 2 cut(s) 175, 217
HpyAV CCTTC 3 cut(s) 59, 69, 433
HpyCH4III ACNGT 2 cut(s) 67, 232
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 1 cut(s) 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 2 cut(s) 245, 322
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 1 cut(s) 196
HspAI GCGC 1 cut(s) 473
Kzo9I GATC 1 cut(s) 331
LguI GCTCTTC 1 cut(s) 282
LpnPI CCDG 7 cut(s) 107, 124, 164, 191, 321, 327, 447
Lsp1109I GCAGC 1 cut(s) 391
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 67, 303, 416
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 8 cut(s) 16, 47, 112, 177, 299, 330, 413, 466
MlsI TGGCCA 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 176
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MseI TTAA 1 cut(s) 261
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 111, 179
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 111
NciI CCSGG 1 cut(s) 111
NdeII GATC 1 cut(s) 331
NlaIII CATG 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 167
PceI AGGCCT 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 208, 280, 318
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 3 cut(s) 116, 164, 381
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 177
PspEI GGTNACC 1 cut(s) 303
PspGI CCWGG 1 cut(s) 177
PspPI GGNCC 2 cut(s) 160, 172
PspXI VCTCGAGB 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 3 cut(s) 115, 163, 380
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 2 cut(s) 160, 172
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 2 cut(s) 111, 179
SetI ASST 8 cut(s) 74, 90, 135, 174, 180, 251, 294, 384
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 172
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 167, 423
SmoI CTYRAG 2 cut(s) 167, 423
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 2 cut(s) 114, 163
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 2 cut(s) 109, 177
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 2 cut(s) 67, 232
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 150, 168, 204, 222
TauI GCSGC 2 cut(s) 117, 165
TfiI GAWTC 3 cut(s) 208, 280, 318
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 441, 466
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 2 cut(s) 441, 466
Van91I CCANNNNNTGG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.