Rroxscaffold_1G00070800

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010974
Physical Location & Seq
Forward (+)
91750574 .. 91751784
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070800.1

Sequence Viewer

Length: 501 bp
ATGTCTCTGACACCCGTATCTCTCTTCACTCTCTCTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCATCTTCCTTCCACAGTCGCCTCATCTCTCCACAACCTCCTCCAAAGCCAAGGCCTCCCCGGCGGCCTCTTCCCCAACAACGTCAAGTCCTACAATCTCGACCCGATGGGCCGCCTCGAGGTCCACCTGGACTCTTCTTGCATGGCCAAGTTCGAGAATCGGGTCTACTTCGACAACACGGTCAGAGCCAATCTCAGCTTTGGTGTGCTTAAAGGCTTGGAGGGTCTGATTCAAGAAGAGCTGTTTCTGTGGTTACCAGTCAAAGGAATCTCAGTTAGTGATCCATCTTCTGGTTTGATTTTGTTTGATATTGGTGTTGCCCATAAGCAGCTTTCTCTTTCTCTCTTTGAAGACCCTCCTCTTTGTAACCCTCAAGAGGTTTCACTGAAGGAGGATGTGGTGAAGAACACTGGATTTGAGGCGCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

166

Amino Acids

18.24

Weight (kDa)

5.25

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 34 - 140 1.9e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61667 AT1G61667
fragaria_vesca FvH4_3g03180
malus_domestica MD05G1334600.v1.1 MD10G1309500.v1.1
prunus_persica Prupe.4G030700_v2.0.a1
pyrus_communis pycom05g30610 pycom10g26090
rosa_chinensis RchiOBHm_Chr5g0004811
rosa_laevigata RLG00000031262
rosa_multiflora Rmu_co7972276.1_g000001 Rmu_sc0008308.1_g000015 Rmu_sc0010871.1_g000007
rosa_roxburghii Rroxscaffold_1G00070800
rosa_rugosa Rorug04G0414000
rosa_samantha Rh5AG041800 Rh5BG041500 Rh5CG045400 Rh5DG041900
rosa_wichuraiana Rw5G004050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AbsI CCTCGAGG 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 359
AccI GTMKAC 1 cut(s) 234
AciI CCGC 2 cut(s) 132, 181
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 213
AcuI CTGAAG 1 cut(s) 476
AfiI CCNNNNNNNGG 4 cut(s) 187, 332, 359, 445
AgsI TTSAA 2 cut(s) 302, 419
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 3 cut(s) 267, 310, 400
AluI AGCT 3 cut(s) 267, 310, 400
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 4 cut(s) 121, 133, 178, 213
ApeKI GCWGC 1 cut(s) 397
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 2 cut(s) 178, 190
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 1 cut(s) 190
BaeI ACNNNNGTAYC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 409
BccI CCATC 2 cut(s) 169, 361
BciT130I CCWGG 1 cut(s) 197
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 9
BglI GCCNNNNNGGC 1 cut(s) 129
BisI GCNGC 3 cut(s) 133, 181, 398
BlsI GCNGC 3 cut(s) 134, 182, 399
Bme1390I CCNGG 2 cut(s) 129, 197
Bme18I GGWCC 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 2 cut(s) 178, 190
BmrFI CCNGG 2 cut(s) 129, 197
BpiI GAAGAC 1 cut(s) 426
BplI GAGNNNNNCTC 2 cut(s) 246, 278
BpuEI CTTGAG 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 118, 127
Bsc4I CCNNNNNNNGG 4 cut(s) 187, 332, 359, 445
Bse1I ACTGG 2 cut(s) 326, 484
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 118, 127
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 4 cut(s) 187, 332, 359, 445
BseMII CTCAG 2 cut(s) 277, 354
BseNI ACTGG 2 cut(s) 326, 484
BseXI GCAGC 1 cut(s) 409
BshFI GGCC 4 cut(s) 123, 135, 180, 215
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 129
BslI CCNNNNNNNGG 4 cut(s) 187, 332, 359, 445
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 4 cut(s) 123, 135, 180, 215
BsoBI CYCGRG 1 cut(s) 185
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 2 cut(s) 132, 181
BspANI GGCC 4 cut(s) 123, 135, 180, 215
BspCNI CTCAG 2 cut(s) 276, 353
BspPI GGATC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 300
BsrI ACTGG 2 cut(s) 326, 484
BssECI CCNNGG 2 cut(s) 118, 127
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 85, 250
Bst6I CTCTTC 4 cut(s) 29, 143, 208, 300
BstDEI CTNAG 2 cut(s) 263, 340
BstEII GGTNACC 1 cut(s) 321
BstF5I GGATG 1 cut(s) 469
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNI CCWGG 1 cut(s) 197
BstPI GGTNACC 1 cut(s) 321
BstSCI CCNGG 2 cut(s) 127, 195
BstV1I GCAGC 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 426
BsuRI GGCC 4 cut(s) 123, 135, 180, 215
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 452, 477
CfoI GCGC 1 cut(s) 493
Cfr13I GGNCC 2 cut(s) 178, 190
CviAII CATG 1 cut(s) 211
DdeI CTNAG 2 cut(s) 263, 340
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 4 cut(s) 29, 143, 208, 300
EarI CTCTTC 4 cut(s) 29, 143, 208, 300
Eco130I CCWWGG 1 cut(s) 118
Eco147I AGGCCT 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 185
Eco91I GGTNACC 1 cut(s) 321
EcoO65I GGTNACC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 214
FaiI YATR 2 cut(s) 212, 393
FatI CATG 1 cut(s) 210
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 133, 181, 398
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 3 cut(s) 133, 181, 398
GlaI GCGC 1 cut(s) 492
GluI GCNGC 3 cut(s) 133, 181, 398
HaeIII GGCC 4 cut(s) 123, 135, 180, 215
HapII CCGG 1 cut(s) 129
HhaI GCGC 1 cut(s) 493
Hin1II CATG 1 cut(s) 214
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HinfI GANTC 4 cut(s) 200, 226, 298, 336
HpaII CCGG 1 cut(s) 129
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 2 cut(s) 193, 235
Hpy188I TCNGA 3 cut(s) 9, 254, 297
Hpy188III TCNNGA 4 cut(s) 167, 223, 302, 443
Hpy8I GTNNAC 2 cut(s) 193, 235
HpyAV CCTTC 3 cut(s) 77, 87, 451
HpyCH4III ACNGT 2 cut(s) 85, 250
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 2 cut(s) 263, 340
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 1 cut(s) 214
HspAI GCGC 1 cut(s) 491
Kzo9I GATC 1 cut(s) 349
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 6 cut(s) 142, 182, 209, 339, 345, 465
Lsp1109I GCAGC 1 cut(s) 409
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 2 cut(s) 321, 434
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 8 cut(s) 16, 65, 130, 195, 317, 348, 431, 484
MlsI TGGCCA 1 cut(s) 215
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 1 cut(s) 194
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 2 cut(s) 129, 197
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 129
NdeII GATC 1 cut(s) 349
NlaIII CATG 1 cut(s) 214
PaeR7I CTCGAG 1 cut(s) 185
PceI AGGCCT 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 300
PfeI GAWTC 3 cut(s) 226, 298, 336
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 3 cut(s) 134, 182, 399
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 195
PspEI GGTNACC 1 cut(s) 321
PspGI CCWGG 1 cut(s) 195
PspPI GGNCC 2 cut(s) 178, 190
PspXI VCTCGAGB 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 3 cut(s) 133, 181, 398
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 2 cut(s) 178, 190
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 129, 197
SetI ASST 8 cut(s) 108, 153, 192, 198, 269, 312, 402, 450
Sfr274I CTCGAG 1 cut(s) 185
SinI GGWCC 1 cut(s) 190
SlaI CTCGAG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 185, 441
SmoI CTYRAG 2 cut(s) 185, 441
SseBI AGGCCT 1 cut(s) 123
SsiI CCGC 2 cut(s) 132, 181
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 2 cut(s) 127, 195
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 85, 250
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 168, 186, 222, 240
TauI GCSGC 2 cut(s) 135, 183
TfiI GAWTC 3 cut(s) 226, 298, 336
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 459, 484
TseI GCWGC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 59
TspRI CASTG 2 cut(s) 459, 484
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.