Rh5BG041500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010974
Physical Location & Seq
Reverse (-)
3050319 .. 3050896
578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG041500.1

Sequence Viewer

Length: 486 bp
ATGTCTCTGACACCCGTATCTCTCTTCACTCTCTCTCTCCTCCTCCTCCTCCTTCATCTTCCTTCCACAGTCACCTCATCTCTCCACAACCTCCTCCAGAGCCAAGGCCTCCCCGGCGGCCTCTTCCCCAACAACGTCAAGTCCTACAATCTCGACCCGATGGGCCGCCTCGAGGTCCAGCTGGACTCTTCTTGCATGGCCAAGTTCGAGAACCGGGTCTACTTCGACAACACAGTCAGAGCCAATCTCAGCTTTGGTGTACTTAAAGGCTTGGAGGGTCTGATTCAAGAAGAGCTGTTTCTGTGGTTACCAGTCAAAGGAATCTCAGTTAGTGATCCATCATCTGGTTTGATTTTGTTTGATATTGGTGTTGCCCATAAGCAGCTTTCTCTTTCTCTCTTTGAAGACCCTCCTCTTTGTAACCCTCAAGGGGTTTCACTGAAGGAGGATGTGGTGAAGAACACTGGATTTGAGGCGCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

161

Amino Acids

17.63

Weight (kDa)

5.26

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 29 - 135 2.6e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61667 AT1G61667
fragaria_vesca FvH4_3g03180
malus_domestica MD05G1334600.v1.1 MD10G1309500.v1.1
prunus_persica Prupe.4G030700_v2.0.a1
pyrus_communis pycom05g30610 pycom10g26090
rosa_chinensis RchiOBHm_Chr5g0004811
rosa_laevigata RLG00000031262
rosa_multiflora Rmu_co7972276.1_g000001 Rmu_sc0008308.1_g000015 Rmu_sc0010871.1_g000007
rosa_roxburghii Rroxscaffold_1G00070800
rosa_rugosa Rorug04G0414000
rosa_samantha Rh5AG041800 Rh5BG041500 Rh5CG045400 Rh5DG041900
rosa_wichuraiana Rw5G004050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AbsI CCTCGAGG 1 cut(s) 170
AccB7I CCANNNNNTGG 1 cut(s) 344
AccI GTMKAC 1 cut(s) 219
AciI CCGC 2 cut(s) 117, 166
AclWI GGATC 1 cut(s) 329
AcoI YGGCCR 1 cut(s) 198
AcuI CTGAAG 1 cut(s) 461
AfaI GTAC 1 cut(s) 261
AfiI CCNNNNNNNGG 4 cut(s) 172, 317, 344, 430
AgsI TTSAA 2 cut(s) 287, 404
AluBI AGCT 4 cut(s) 181, 252, 295, 385
AluI AGCT 4 cut(s) 181, 252, 295, 385
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 4 cut(s) 106, 118, 163, 198
ApeKI GCWGC 1 cut(s) 382
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 2 cut(s) 163, 175
AsuC2I CCSGG 2 cut(s) 114, 215
AsuHPI GGTGA 2 cut(s) 64, 466
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 175
BaeI ACNNNNGTAYC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 394
BccI CCATC 2 cut(s) 154, 346
BcnI CCSGG 2 cut(s) 114, 215
BcoDI GTCTC 1 cut(s) 9
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 3 cut(s) 118, 166, 383
BlsI GCNGC 3 cut(s) 119, 167, 384
Bme1390I CCNGG 2 cut(s) 114, 215
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 163, 175
BmrFI CCNGG 2 cut(s) 114, 215
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 231, 263
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 411
BpuMI CCSGG 2 cut(s) 114, 215
BsaJI CCNNGG 2 cut(s) 103, 112
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 317, 344, 430
Bse1I ACTGG 2 cut(s) 311, 469
BseDI CCNNGG 2 cut(s) 103, 112
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 4 cut(s) 172, 317, 344, 430
BseMII CTCAG 2 cut(s) 262, 339
BseNI ACTGG 2 cut(s) 311, 469
BseRI GAGGAG 6 cut(s) 29, 32, 35, 38, 83, 402
BseXI GCAGC 1 cut(s) 394
BshFI GGCC 4 cut(s) 108, 120, 165, 200
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 114, 214
BslI CCNNNNNNNGG 4 cut(s) 172, 317, 344, 430
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 4 cut(s) 108, 120, 165, 200
BsoBI CYCGRG 1 cut(s) 170
Bsp143I GATC 1 cut(s) 334
BspACI CCGC 2 cut(s) 117, 166
BspANI GGCC 4 cut(s) 108, 120, 165, 200
BspCNI CTCAG 2 cut(s) 261, 338
BspPI GGATC 1 cut(s) 329
BspQI GCTCTTC 1 cut(s) 285
BsrI ACTGG 2 cut(s) 311, 469
BssECI CCNNGG 2 cut(s) 103, 112
BssMI GATC 1 cut(s) 334
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 2 cut(s) 70, 235
Bst6I CTCTTC 4 cut(s) 29, 128, 193, 285
BstDEI CTNAG 2 cut(s) 248, 325
BstEII GGTNACC 1 cut(s) 306
BstF5I GGATG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 334
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 112, 213
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 411
BsuRI GGCC 4 cut(s) 108, 120, 165, 200
BtsCI GGATG 1 cut(s) 454
BtsIMutI CAGTG 2 cut(s) 437, 462
CfoI GCGC 1 cut(s) 478
Cfr13I GGNCC 2 cut(s) 163, 175
Csp6I GTAC 1 cut(s) 260
CviAII CATG 1 cut(s) 196
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 2 cut(s) 248, 325
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 4 cut(s) 29, 128, 193, 285
EarI CTCTTC 4 cut(s) 29, 128, 193, 285
Eco130I CCWWGG 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 1 cut(s) 199
FaiI YATR 2 cut(s) 197, 378
FatI CATG 1 cut(s) 195
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 118, 166, 383
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 118, 166, 383
GlaI GCGC 1 cut(s) 477
GluI GCNGC 3 cut(s) 118, 166, 383
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 4 cut(s) 108, 120, 165, 200
HapII CCGG 2 cut(s) 114, 214
HhaI GCGC 1 cut(s) 478
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HinfI GANTC 3 cut(s) 185, 283, 321
HpaII CCGG 2 cut(s) 114, 214
HphI GGTGA 2 cut(s) 64, 466
Hpy166II GTNNAC 2 cut(s) 220, 260
Hpy188I TCNGA 3 cut(s) 9, 239, 282
Hpy188III TCNNGA 4 cut(s) 97, 152, 208, 287
Hpy8I GTNNAC 2 cut(s) 220, 260
HpyAV CCTTC 3 cut(s) 62, 72, 436
HpyCH4III ACNGT 2 cut(s) 70, 235
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 2 cut(s) 248, 325
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 476
Kzo9I GATC 1 cut(s) 334
LguI GCTCTTC 1 cut(s) 285
LpnPI CCDG 8 cut(s) 110, 127, 167, 191, 227, 324, 330, 450
Lsp1109I GCAGC 1 cut(s) 394
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 70, 306, 419
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 7 cut(s) 16, 50, 115, 180, 302, 416, 469
MlsI TGGCCA 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 179
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
MseI TTAA 1 cut(s) 264
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 2 cut(s) 114, 214
MspR9I CCNGG 2 cut(s) 114, 215
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 2 cut(s) 114, 215
NdeII GATC 1 cut(s) 334
NlaIII CATG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 170
PceI AGGCCT 1 cut(s) 108
PciSI GCTCTTC 1 cut(s) 285
PfeI GAWTC 2 cut(s) 283, 321
PflMI CCANNNNNTGG 1 cut(s) 344
PkrI GCNGC 3 cut(s) 119, 167, 384
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PspEI GGTNACC 1 cut(s) 306
PspPI GGNCC 2 cut(s) 163, 175
PspXI VCTCGAGB 1 cut(s) 170
PsrI GAACNNNNNNTAC 2 cut(s) 203, 235
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 3 cut(s) 118, 166, 383
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 2 cut(s) 163, 175
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 114, 215
SetI ASST 8 cut(s) 77, 93, 138, 177, 183, 254, 297, 387
Sfr274I CTCGAG 1 cut(s) 170
SinI GGWCC 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 170
SmlI CTYRAG 2 cut(s) 170, 426
SmoI CTYRAG 2 cut(s) 170, 426
SseBI AGGCCT 1 cut(s) 108
SsiI CCGC 2 cut(s) 117, 166
StuI AGGCCT 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 112, 213
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 2 cut(s) 70, 235
TaiI ACGT 1 cut(s) 138
TaqI TCGA 4 cut(s) 153, 171, 207, 225
TatI WGTACW 1 cut(s) 259
TauI GCSGC 2 cut(s) 120, 168
TfiI GAWTC 2 cut(s) 283, 321
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 2 cut(s) 444, 469
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 44
TspRI CASTG 2 cut(s) 444, 469
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.