MD05G1338800.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011139
Physical Location & Seq
Forward (+)
46013841 .. 46017085
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1338800.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGACTGGAGGGTCATTGGGGATTCGCTCAGGGAGTTATGGGTCTTTGGATAAACAGCTACAGAACAACAACATCAACAGCATCAACGGAGTGTTGCCAATCCAATCTGCAAGGAAGCCGTCGAAAATGCTCAAGGAGAAGGATAGGTTGTTCCATTGGATCTTCAAGTTTGCCGGCCGGAAGAAGGTTGGAATGCTGCTGCTCTGCCTCATCTCCGCCGCAGTTTTCGTCTGGGTTTTGTACGTTGGAAAAGGTGAAGATGGACAAGATGTTGTCAATGTCGGAAACATTAGTGTTAACAACAGCATCCCTTTAAGTTTTTCTGAATCCTCACGACAACATGGAGCTCAAAGTATCAACATGACTTTTCTGCTGGCGTTGCCCCCTCCTCCTCCTGCTGTTTTTCTGGGTTACACTCTTCCTCCTGGGCATCCATGTAATACATTCACTTTGCCTCCCCCACCAGCAGATAAAAAAAGAACTGGACCACGGCCTTGTCCAGTATGTTACCTTCCTGTGGAAGAAGCCATAGCCTTGATGCCGAAGGTCCCTTCATATTCTCCGGTTCTTAAGAATTTAACATACATTTATGAAGAGAATTTATCAAGAGACACAGAATTTGGAGGCTCAGATTTTGGTGGATACCCTACATTGAAGCAAAGGAATGATTCTTATGATATAAGAGAGTCAATGAGTGTGCACTGCGGATTTGTTAGAGGAAGTAAACCTGGGCGGAATACAGGATATGATATGGATGAGGTTGACCTAAATGATATGGAGCAGTGTAATGGAGTGGTTGTTGCATCGGCTATATTCGGAAATTTTGATGAGATAAACCAGCCAAGGAACATTAGTGATTATTCCAAGGAGACCGTTTGCTTCTATATGTTTATAGATAATGTGACAGAAGCTTACTTGAGGACATCTGGAAAAATGGGCAGCGATCGTAAAGTTGGTATATGGAGAACTGTGGTTGTGCATAATCCTCCTTACACAGATGGAAGGCGCACTGGGAAGATTCCCAAGCTTCTAGTGCATAGGATGTTTCCTAATGCCCGCTTTTCTTTATGGATTGATGGGAAACTTGAACTTGTCGTGGATCCATATCAAATTCTTGAAAGGTATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

377

Amino Acids

41.96

Weight (kDa)

8.81

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 193 - 376 4.7e-79 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28240
fragaria_vesca FvH4_3g09110 FvH4_3g09110 FvH4_3g09110 FvH4_3g09110
malus_domestica MD05G1338800.v1.1 MD10G1293800.v1.1
prunus_persica Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1
pyrus_communis pycom05g29010 pycom10g24570
rosa_chinensis RchiOBHm_Chr5g0008541
rosa_laevigata RLG00000031578
rosa_multiflora Rmu_sc0001665.1_g000015
rosa_roxburghii Rroxscaffold_1G00068020
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5BG062700 Rh5DG063400
rosa_wichuraiana Rw5G005560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AciI CCGC 5 cut(s) 216, 219, 705, 733, 1057
AclWI GGATC 3 cut(s) 167, 1094, 1107
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 5 cut(s) 574, 598, 617, 820, 1110
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 184, 517
AflII CTTAAG 1 cut(s) 569
AgsI TTSAA 4 cut(s) 166, 655, 1088, 1118
AjnI CCWGG 2 cut(s) 424, 727
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
AluBI AGCT 4 cut(s) 58, 347, 911, 1027
AluI AGCT 4 cut(s) 58, 347, 911, 1027
Alw21I GWGCWC 2 cut(s) 349, 702
Alw26I GTCTC 2 cut(s) 603, 863
Alw44I GTGCAC 1 cut(s) 698
AlwI GGATC 3 cut(s) 167, 1094, 1107
AoxI GGCC 2 cut(s) 175, 491
ApaLI GTGCAC 1 cut(s) 698
ApeKI GCWGC 3 cut(s) 196, 199, 939
ApoI RAATTY 5 cut(s) 574, 598, 617, 820, 1110
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AspLEI GCGC 1 cut(s) 1008
AspS9I GGNCC 2 cut(s) 485, 547
AsuHPI GGTGA 1 cut(s) 266
AvaII GGWCC 2 cut(s) 485, 547
BaeGI GKGCMC 1 cut(s) 702
BamHI GGATCC 1 cut(s) 1099
BanII GRGCYC 1 cut(s) 349
BarI GAAGNNNNNNTAC 2 cut(s) 495, 527
Bbv12I GWGCWC 2 cut(s) 349, 702
BbvI GCAGC 3 cut(s) 183, 186, 951
BccI CCATC 3 cut(s) 254, 992, 1070
BceAI ACGGC 2 cut(s) 103, 506
BciT130I CCWGG 2 cut(s) 426, 729
BciVI GTATCC 1 cut(s) 635
BcoDI GTCTC 2 cut(s) 603, 863
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 569
BfuI GTATCC 1 cut(s) 635
BisI GCNGC 4 cut(s) 197, 200, 219, 940
BlsI GCNGC 4 cut(s) 198, 201, 220, 941
Bme1390I CCNGG 2 cut(s) 426, 729
Bme18I GGWCC 2 cut(s) 485, 547
BmgT120I GGNCC 2 cut(s) 485, 547
BmiI GGNNCC 2 cut(s) 549, 1101
BmrFI CCNGG 2 cut(s) 426, 729
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 5 cut(s) 90, 315, 439, 528, 812
BmuI ACTGGG 1 cut(s) 1020
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 116, 937
BsaBI GATNNNNATC 1 cut(s) 1104
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 5 cut(s) 425, 488, 728, 842, 864
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 6 cut(s) 406, 436, 439, 469, 783, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 517
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 4 cut(s) 10, 487, 500, 1015
Bse8I GATNNNNATC 1 cut(s) 1104
BseBI CCWGG 2 cut(s) 426, 729
BseDI CCNNGG 5 cut(s) 425, 488, 728, 842, 864
BseGI GGATG 4 cut(s) 306, 430, 760, 1047
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 2 cut(s) 184, 517
BseMII CTCAG 2 cut(s) 42, 642
BseNI ACTGG 4 cut(s) 10, 487, 500, 1015
BseRI GAGGAG 2 cut(s) 378, 381
BseSI GKGCMC 1 cut(s) 702
BseX3I CGGCCG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 183, 186, 951
Bsh1285I CGRYCG 2 cut(s) 178, 946
BshFI GGCC 2 cut(s) 177, 493
BsiEI CGRYCG 2 cut(s) 178, 946
BsiHKAI GWGCWC 2 cut(s) 349, 702
BsiSI CCGG 3 cut(s) 174, 178, 563
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 2 cut(s) 184, 517
BsmAI GTCTC 2 cut(s) 603, 863
BsmFI GGGAC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 2 cut(s) 177, 493
Bso31I GGTCTC 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 349, 702
Bsp143I GATC 3 cut(s) 159, 943, 1099
BspACI CCGC 5 cut(s) 216, 219, 705, 733, 1057
BspANI GGCC 2 cut(s) 177, 493
BspCNI CTCAG 2 cut(s) 41, 641
BspLI GGNNCC 2 cut(s) 549, 1101
BspPI GGATC 3 cut(s) 167, 1094, 1107
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 863
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 4 cut(s) 10, 487, 500, 1015
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 5 cut(s) 425, 488, 728, 842, 864
BssMI GATC 3 cut(s) 159, 943, 1099
BssT1I CCWWGG 2 cut(s) 842, 864
Bst2UI CCWGG 2 cut(s) 426, 729
Bst4CI ACNGT 2 cut(s) 874, 970
Bst6I CTCTTC 2 cut(s) 423, 588
BstAFI CTTAAG 1 cut(s) 569
BstC8I GCNNGC 3 cut(s) 175, 375, 1057
BstDEI CTNAG 2 cut(s) 28, 628
BstDSI CCRYGG 1 cut(s) 488
BstF5I GGATG 4 cut(s) 306, 430, 760, 1047
BstHHI GCGC 1 cut(s) 1008
BstKTI GATC 3 cut(s) 162, 946, 1102
BstMAI GTCTC 2 cut(s) 603, 863
BstMBI GATC 3 cut(s) 159, 943, 1099
BstMCI CGRYCG 2 cut(s) 178, 946
BstMWI GCNNNNNNNGC 2 cut(s) 379, 1033
BstNI CCWGG 2 cut(s) 426, 729
BstSCI CCNGG 2 cut(s) 424, 727
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 702
BstV1I GCAGC 3 cut(s) 183, 186, 951
BstX2I RGATCY 2 cut(s) 159, 1099
BstYI RGATCY 2 cut(s) 159, 1099
BstZI CGGCCG 1 cut(s) 175
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 2 cut(s) 177, 493
BtgI CCRYGG 1 cut(s) 488
BtsCI GGATG 4 cut(s) 306, 430, 760, 1047
BtsI GCAGTG 2 cut(s) 700, 788
BtsIMutI CAGTG 3 cut(s) 700, 788, 1008
Cac8I GCNNGC 3 cut(s) 175, 375, 1057
CfoI GCGC 1 cut(s) 1008
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 2 cut(s) 485, 547
Csp6I GTAC 1 cut(s) 241
CviAII CATG 3 cut(s) 341, 361, 435
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 2 cut(s) 28, 628
DpnI GATC 3 cut(s) 161, 945, 1101
DpnII GATC 3 cut(s) 159, 943, 1099
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 175
EagI CGGCCG 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 423, 588
EarI CTCTTC 2 cut(s) 423, 588
EciI GGCGGA 2 cut(s) 205, 748
Ecl136II GAGCTC 1 cut(s) 347
EclXI CGGCCG 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 842, 864
Eco24I GRGCYC 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 863
Eco47I GGWCC 2 cut(s) 485, 547
Eco52I CGGCCG 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 424, 727
EcoT14I CCWWGG 2 cut(s) 842, 864
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 2 cut(s) 842, 864
FaeI CATG 3 cut(s) 344, 364, 438
FaqI GGGAC 1 cut(s) 533
FatI CATG 3 cut(s) 340, 360, 434
FauI CCCGC 1 cut(s) 1064
Fnu4HI GCNGC 4 cut(s) 197, 200, 219, 940
FokI GGATG 4 cut(s) 293, 417, 767, 1054
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 4 cut(s) 197, 200, 219, 940
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 1 cut(s) 1007
GluI GCNGC 4 cut(s) 197, 200, 219, 940
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 2 cut(s) 177, 493
HapII CCGG 3 cut(s) 174, 178, 563
HhaI GCGC 1 cut(s) 1008
Hin1II CATG 3 cut(s) 344, 364, 438
Hin6I GCGC 1 cut(s) 1006
HinP1I GCGC 1 cut(s) 1006
HincII GTYRAC 2 cut(s) 298, 763
HindII GTYRAC 2 cut(s) 298, 763
HindIII AAGCTT 2 cut(s) 909, 1025
HinfI GANTC 5 cut(s) 22, 326, 668, 686, 1018
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 3 cut(s) 174, 178, 563
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 4 cut(s) 298, 700, 725, 763
Hpy188I TCNGA 5 cut(s) 284, 325, 631, 818, 1130
Hpy188III TCNNGA 4 cut(s) 333, 606, 927, 1115
Hpy8I GTNNAC 4 cut(s) 298, 700, 725, 763
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 6 cut(s) 133, 178, 521, 538, 561, 996
HpyCH4III ACNGT 2 cut(s) 874, 970
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 110, 700, 803, 979, 1036
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 1033
HpyF3I CTNAG 2 cut(s) 28, 628
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 344, 364, 438
HspAI GCGC 1 cut(s) 1006
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 3 cut(s) 159, 943, 1099
LmnI GCTCC 2 cut(s) 344, 778
Lsp1109I GCAGC 3 cut(s) 183, 186, 951
LweI GCATC 5 cut(s) 90, 315, 439, 528, 812
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 3 cut(s) 410, 506, 901
MalI GATC 3 cut(s) 161, 945, 1101
MboI GATC 3 cut(s) 159, 943, 1099
MboII GAAGA 7 cut(s) 154, 193, 269, 410, 533, 605, 1027
MflI RGATCY 2 cut(s) 159, 1099
MhlI GDGCHC 2 cut(s) 349, 702
MluCI AATT 5 cut(s) 574, 598, 617, 820, 1110
MlyI GAGTC 1 cut(s) 695
MmeI TCCRAC 3 cut(s) 169, 226, 262
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 4 cut(s) 297, 314, 570, 578
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 3 cut(s) 174, 178, 563
MspR9I CCNGG 2 cut(s) 426, 729
Mva1269I GAATGC 1 cut(s) 198
MvaI CCWGG 2 cut(s) 426, 729
MwoI GCNNNNNNNGC 2 cut(s) 379, 1033
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 3 cut(s) 159, 943, 1099
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 344, 364, 438
NlaIV GGNNCC 2 cut(s) 549, 1101
NmuCI GTSAC 1 cut(s) 901
PctI GAATGC 1 cut(s) 198
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 4 cut(s) 22, 326, 668, 1018
PkrI GCNGC 4 cut(s) 198, 201, 220, 941
Ple19I CGATCG 1 cut(s) 946
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PpuMI RGGWCCY 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 424, 727
PspGI CCWGG 2 cut(s) 424, 727
PspN4I GGNNCC 2 cut(s) 549, 1101
PspPI GGNCC 2 cut(s) 485, 547
PspPPI RGGWCCY 1 cut(s) 547
PsuI RGATCY 2 cut(s) 159, 1099
PvuI CGATCG 1 cut(s) 946
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 4 cut(s) 297, 314, 570, 578
SatI GCNGC 4 cut(s) 197, 200, 219, 940
Sau3AI GATC 3 cut(s) 159, 943, 1099
Sau96I GGNCC 2 cut(s) 485, 547
SchI GAGTC 1 cut(s) 695
ScrFI CCNGG 2 cut(s) 426, 729
SduI GDGCHC 2 cut(s) 349, 702
SfaNI GCATC 5 cut(s) 90, 315, 439, 528, 812
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 2 cut(s) 485, 547
SmlI CTYRAG 3 cut(s) 131, 569, 916
SmoI CTYRAG 3 cut(s) 131, 569, 916
Sse9I AATT 5 cut(s) 574, 598, 617, 820, 1110
SsiI CCGC 5 cut(s) 216, 219, 705, 733, 1057
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 2 cut(s) 424, 727
StyI CCWWGG 2 cut(s) 842, 864
TaaI ACNGT 2 cut(s) 874, 970
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 122
TasI AATT 5 cut(s) 574, 598, 617, 820, 1110
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 4 cut(s) 22, 326, 668, 1018
Tru1I TTAA 4 cut(s) 297, 314, 570, 578
Tru9I TTAA 4 cut(s) 297, 314, 570, 578
TscAI CASTG 3 cut(s) 707, 788, 1015
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 3 cut(s) 196, 199, 939
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 2 cut(s) 543, 606
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 3 cut(s) 707, 788, 1015
Vha464I CTTAAG 1 cut(s) 569
VneI GTGCAC 1 cut(s) 698
VpaK11BI GGWCC 2 cut(s) 485, 547
XapI RAATTY 5 cut(s) 574, 598, 617, 820, 1110
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.