Prupe.4G048000_v2.0.a1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0011139
Physical Location & Seq
Reverse (-)
2289202 .. 2293658
4457 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G048000.1

Sequence Viewer

Length: 1752 bp
ATGACTGGAGGGTCATTGGGGATTCGTTCAGGGAGTTATGGGTCTTTGGATAAACAGCTACAGAACAACAACAACGGAGTGTCGCCAATCCAATCTGCACGTAAGCCTTCAAAGATGCTCAAGGAGAAGGAGAGGTTGTTCCATTGGATCTGCAAGTTTGCCGGCCGCAAAAAGGTTGGAATGTTGTTCCTCTGTCTCATCTCCGCCGCGGTTTTCGTCTGGGTTTTGTACGTTGGAAAAGGTGAAGATGAACAAGGCGTCAGTGTCGAAAACATTAGTGTTAACAATAGCATCACTATAAGTTATCTTGGATCCTTGCAAACACATGGACCGCAAAGTAACAACGTGACTTTTTCGCTAGCATTGCCTCCTCCTCCCCCTGCTGTATTTCTGGGTTACACCCTTCCTCCTGGGCATCCATGTAATCTATTCACATTGCCTCCCCCACCAGCAGATAAAAAAAGAACTGGACCACGGCCTTGTCCAGTATGTTACCTTCCTGTGGAGGAAGCCATAGCCTTGATGCCAAAGGTCCCTTCAGATTCTCCTGTTCTTAAGAATTTAACATACATTTATGAAGAAAATTTGTCTAGAGAAACAGAATTTGGAGGCTCAGACTTTGGTGGATATCCTACTTTGCAGCAAAGGAGTGATTCTTATGATATAAGAGAGTCAATGAGTGTGCACTGTGGATTTGTTGGTGGAAGTAAACCTGGACGCAATACAGGATATGATATGGATGAGGTTGACCTACATGATATGGAGCAGTGTCATGGTGTGGTTGTTGCATCAGCTATATTTGGGAATTTTGATGAGATAAACCAGCCGACAAACATTAGTGATTATTCCAAGGAGACCGTTTGCTTCTACATGTTTATAGATGAGGTGACGGAAGCTTATTTGAGGAGTTCTGGAAAACTGGGCAGCGATAGGAAAGTTGGTATATGGAGAACTGTTGTTGTGCATAACCCTCCTTATACGGATGGAAGGCGGACTGGGAAGATTCCTAAGCTTCTAGTGCATAGGATGTTTCCAAATGCCCGCTTTTCTTTATGGATTGATGGGAAGCTTGAACTTGTCGTGGATCCATATCAAATTCTTGAAAGGTTCTTGTGGAGAAAAAATGTAACTTTTGCAATTTCTAAACATTATAAACGTTTTGACGTGTTTACGGAAGCTGAGGCAAATAAAGCAGCTGGAAAATACAAGAATGCTTCTATTGACTTCCAGATTGAGTTTTACAAAAAGGAAGGTTTGACCCCATATTCCGAGGCAAAGCTTCCTATTATAAGTGATGTTCCTGAAGGATGTGTAATAATAAGGGAGCACGTCCCTATCAGCAACCTTCTCACTTGTCTTTGGTTCAATGAAGTTGATCGTTTTACTTCCAGGGACCAAATTAGTTTCTCCACTGTCAGAGACCACATCAGGGAAAGGACAAACTGGACTATCAATATGTTCTACGACTGTGAAAGGCGCAATTTTGTTGTTCAGAAATATCATAGAGATGTTTTGGAGCACCTGGCGCCTCGTGTTCCTGTGGCTGTTCCTCCACCACCACTATTACCGCCGCCGCCGCCGCCACCAGTTATAAGTAACGAACCACCAGTTCAACCATCAGTCGAAACTTTGCCCGAAAGGGTTGTAAATGGCCCAGGTAAGAGGGGCCCAAGGCGCGGGAGAGATCGAAGGTCTGGTTCTAGGCGTCACCGCAAAGTTGTGGCCGGTAACAGAGACATTGATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

584

Amino Acids

65.61

Weight (kDa)

8.92

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28240
fragaria_vesca FvH4_3g09110 FvH4_3g09110 FvH4_3g09110 FvH4_3g09110
malus_domestica MD05G1338800.v1.1 MD10G1293800.v1.1
prunus_persica Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1
pyrus_communis pycom05g29010 pycom10g24570
rosa_chinensis RchiOBHm_Chr5g0008541
rosa_laevigata RLG00000031578
rosa_multiflora Rmu_sc0001665.1_g000015
rosa_roxburghii Rroxscaffold_1G00068020
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5BG062700 Rh5DG063400
rosa_wichuraiana Rw5G005560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1152, 1289, 1592
AasI GACNNNNNNGTC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 1525
AccII CGCG 2 cut(s) 209, 1677
AclI AACGTT 1 cut(s) 1156
AclWI GGATC 5 cut(s) 155, 306, 319, 1079, 1092
AcoI YGGCCR 2 cut(s) 163, 1722
AcsI RAATTY 5 cut(s) 559, 583, 602, 805, 1095
AcuI CTGAAG 2 cut(s) 522, 1323
AcyI GRCGYC 3 cut(s) 258, 1526, 1705
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 4 cut(s) 172, 502, 1429, 1676
AflII CTTAAG 1 cut(s) 554
AflIII ACRYGT 2 cut(s) 870, 1164
AgsI TTSAA 6 cut(s) 111, 1073, 1103, 1366, 1613, 1746
AjiI CACGTC 2 cut(s) 1165, 1330
AjnI CCWGG 5 cut(s) 409, 712, 1388, 1521, 1654
AjuI GAANNNNNNNTTGG 4 cut(s) 520, 552, 588, 620
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 8 cut(s) 58, 794, 896, 1012, 1069, 1178, 1196, 1279
AluI AGCT 8 cut(s) 58, 794, 896, 1012, 1069, 1178, 1196, 1279
Alw21I GWGCWC 3 cut(s) 687, 1329, 1521
Alw26I GTCTC 4 cut(s) 200, 848, 1413, 1728
Alw44I GTGCAC 1 cut(s) 683
AlwI GGATC 5 cut(s) 155, 306, 319, 1079, 1092
AoxI GGCC 5 cut(s) 163, 476, 1651, 1666, 1722
ApaI GGGCCC 1 cut(s) 1670
ApaLI GTGCAC 1 cut(s) 683
ApeKI GCWGC 3 cut(s) 640, 924, 1193
ApoI RAATTY 5 cut(s) 559, 583, 602, 805, 1095
AspLEI GCGC 3 cut(s) 1479, 1528, 1677
AspS9I GGNCC 7 cut(s) 329, 470, 532, 1393, 1652, 1666, 1667
AsuHPI GGTGA 3 cut(s) 254, 898, 1700
AsuNHI GCTAGC 1 cut(s) 358
AvaII GGWCC 4 cut(s) 329, 470, 532, 1393
BaeGI GKGCMC 2 cut(s) 687, 1670
BamHI GGATCC 2 cut(s) 311, 1084
BanI GGYRCC 1 cut(s) 1525
BanII GRGCYC 1 cut(s) 1670
BarI GAAGNNNNNNTAC 2 cut(s) 480, 512
BauI CACGAG 1 cut(s) 1530
Bbv12I GWGCWC 3 cut(s) 687, 1329, 1521
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 3 cut(s) 652, 936, 1205
BccI CCATC 3 cut(s) 977, 1055, 1624
BceAI ACGGC 1 cut(s) 491
BciT130I CCWGG 5 cut(s) 411, 714, 1390, 1523, 1656
BcoDI GTCTC 4 cut(s) 200, 848, 1413, 1728
BfaI CTAG 4 cut(s) 359, 591, 1016, 1701
BfmI CTRYAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 1529
BfrI CTTAAG 1 cut(s) 554
BisI GCNGC 9 cut(s) 166, 207, 641, 925, 1194, 1571, 1574, 1577, 1580
BlsI GCNGC 9 cut(s) 167, 208, 642, 926, 1195, 1572, 1575, 1578, 1581
Bme1390I CCNGG 5 cut(s) 411, 714, 1390, 1523, 1656
Bme18I GGWCC 4 cut(s) 329, 470, 532, 1393
BmgBI CACGTC 2 cut(s) 1165, 1330
BmgT120I GGNCC 7 cut(s) 329, 470, 532, 1393, 1652, 1666, 1667
BmiI GGNNCC 7 cut(s) 313, 534, 1086, 1394, 1527, 1667, 1668
BmrFI CCNGG 5 cut(s) 411, 714, 1390, 1523, 1656
BmrI ACTGGG 2 cut(s) 929, 1005
BmsI GCATC 5 cut(s) 105, 300, 424, 513, 797
BmtI GCTAGC 1 cut(s) 362
BmuI ACTGGG 2 cut(s) 929, 1005
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 2 cut(s) 1008, 1179
BpuEI CTTGAG 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 1089
BsaHI GRCGYC 3 cut(s) 258, 1526, 1705
BsaI GGTCTC 2 cut(s) 848, 1413
BsaJI CCNNGG 8 cut(s) 207, 410, 473, 849, 1269, 1389, 1654, 1670
BsaXI ACNNNNNCTCC 6 cut(s) 122, 152, 391, 421, 424, 454
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 502, 1429, 1676
Bse118I RCCGGY 2 cut(s) 161, 1724
Bse1I ACTGG 8 cut(s) 10, 472, 485, 924, 1000, 1448, 1586, 1607
Bse3DI GCAATG 2 cut(s) 362, 434
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 5 cut(s) 411, 714, 1390, 1523, 1656
BseDI CCNNGG 8 cut(s) 207, 410, 473, 849, 1269, 1389, 1654, 1670
BseGI GGATG 5 cut(s) 415, 745, 988, 1032, 1313
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 4 cut(s) 172, 502, 1429, 1676
BseMI GCAATG 2 cut(s) 362, 434
BseMII CTCAG 2 cut(s) 627, 1170
BseNI ACTGG 8 cut(s) 10, 472, 485, 924, 1000, 1448, 1586, 1607
BseRI GAGGAG 3 cut(s) 360, 363, 919
BseSI GKGCMC 2 cut(s) 687, 1670
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 652, 936, 1205
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 2 cut(s) 209, 1677
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 165, 478, 1653, 1668, 1724
BshNI GGYRCC 1 cut(s) 1525
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 3 cut(s) 687, 1329, 1521
BsiSI CCGG 2 cut(s) 162, 1725
BslFI GGGAC 3 cut(s) 518, 1316, 1406
BslI CCNNNNNNNGG 4 cut(s) 172, 502, 1429, 1676
BsmAI GTCTC 4 cut(s) 200, 848, 1413, 1728
BsmFI GGGAC 3 cut(s) 518, 1316, 1406
BsmI GAATGC 1 cut(s) 1216
BsnI GGCC 5 cut(s) 165, 478, 1653, 1668, 1724
Bso31I GGTCTC 2 cut(s) 848, 1413
Bsp120I GGGCCC 1 cut(s) 1666
Bsp1286I GDGCHC 4 cut(s) 687, 1329, 1521, 1670
Bsp143I GATC 5 cut(s) 147, 311, 1084, 1375, 1684
BspANI GGCC 5 cut(s) 165, 478, 1653, 1668, 1724
BspCNI CTCAG 2 cut(s) 626, 1171
BspFNI CGCG 2 cut(s) 209, 1677
BspLI GGNNCC 7 cut(s) 313, 534, 1086, 1394, 1527, 1667, 1668
BspOI GCTAGC 1 cut(s) 362
BspPI GGATC 5 cut(s) 155, 306, 319, 1079, 1092
BspT107I GGYRCC 1 cut(s) 1525
BspTI CTTAAG 1 cut(s) 554
BspTNI GGTCTC 2 cut(s) 848, 1413
BsrDI GCAATG 2 cut(s) 362, 434
BsrFI RCCGGY 2 cut(s) 161, 1724
BsrI ACTGG 8 cut(s) 10, 472, 485, 924, 1000, 1448, 1586, 1607
BssAI RCCGGY 2 cut(s) 161, 1724
BssECI CCNNGG 8 cut(s) 207, 410, 473, 849, 1269, 1389, 1654, 1670
BssMI GATC 5 cut(s) 147, 311, 1084, 1375, 1684
BssNI GRCGYC 3 cut(s) 258, 1526, 1705
BssSI CACGAG 1 cut(s) 1530
BssT1I CCWWGG 2 cut(s) 849, 1670
Bst2BI CACGAG 1 cut(s) 1530
Bst2UI CCWGG 5 cut(s) 411, 714, 1390, 1523, 1656
Bst4CI ACNGT 5 cut(s) 689, 859, 955, 1414, 1469
BstACI GRCGYC 3 cut(s) 258, 1526, 1705
BstAFI CTTAAG 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 163, 360, 1042
BstDEI CTNAG 3 cut(s) 613, 1008, 1179
BstDSI CCRYGG 2 cut(s) 207, 473
BstF5I GGATG 5 cut(s) 415, 745, 988, 1032, 1313
BstFNI CGCG 2 cut(s) 209, 1677
BstH2I RGCGCY 1 cut(s) 1529
BstHHI GCGC 3 cut(s) 1479, 1528, 1677
BstKTI GATC 5 cut(s) 150, 314, 1087, 1378, 1687
BstMAI GTCTC 4 cut(s) 200, 848, 1413, 1728
BstMBI GATC 5 cut(s) 147, 311, 1084, 1375, 1684
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 7 cut(s) 364, 1018, 1190, 1525, 1576, 1579, 1674
BstNI CCWGG 5 cut(s) 411, 714, 1390, 1523, 1656
BstNSI RCATGY 1 cut(s) 874
BstSCI CCNGG 5 cut(s) 409, 712, 1388, 1521, 1654
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 2 cut(s) 687, 1670
BstUI CGCG 2 cut(s) 209, 1677
BstV1I GCAGC 3 cut(s) 652, 936, 1205
BstX2I RGATCY 3 cut(s) 147, 311, 1084
BstYI RGATCY 3 cut(s) 147, 311, 1084
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 5 cut(s) 165, 478, 1653, 1668, 1724
BtgI CCRYGG 2 cut(s) 207, 473
BtrI CACGTC 2 cut(s) 1165, 1330
BtsCI GGATG 5 cut(s) 415, 745, 988, 1032, 1313
BtsI GCAGTG 1 cut(s) 773
BtsIMutI CAGTG 4 cut(s) 268, 685, 773, 1410
Cac8I GCNNGC 3 cut(s) 163, 360, 1042
CfoI GCGC 3 cut(s) 1479, 1528, 1677
Cfr10I RCCGGY 2 cut(s) 161, 1724
Cfr13I GGNCC 7 cut(s) 329, 470, 532, 1393, 1652, 1666, 1667
Cfr42I CCGCGG 1 cut(s) 210
CseI GACGC 3 cut(s) 247, 726, 1694
Csp6I GTAC 1 cut(s) 229
CviAII CATG 5 cut(s) 326, 420, 755, 773, 871
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 613, 1008, 1179
DinI GGCGCC 1 cut(s) 1527
DpnI GATC 5 cut(s) 149, 313, 1086, 1377, 1686
DpnII GATC 5 cut(s) 147, 311, 1084, 1375, 1684
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 163, 1722
EagI CGGCCG 1 cut(s) 163
EciI GGCGGA 2 cut(s) 193, 1006
EclXI CGGCCG 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 849, 1670
Eco24I GRGCYC 1 cut(s) 1670
Eco31I GGTCTC 2 cut(s) 848, 1413
Eco32I GATATC 1 cut(s) 629
Eco47I GGWCC 4 cut(s) 329, 470, 532, 1393
Eco52I CGGCCG 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 522, 1323
EcoO109I RGGNCCY 2 cut(s) 532, 1666
EcoRII CCWGG 5 cut(s) 409, 712, 1388, 1521, 1654
EcoRV GATATC 1 cut(s) 629
EcoT14I CCWWGG 2 cut(s) 849, 1670
EcoT38I GRGCYC 1 cut(s) 1670
EgeI GGCGCC 1 cut(s) 1527
EheI GGCGCC 1 cut(s) 1527
ErhI CCWWGG 2 cut(s) 849, 1670
FaeI CATG 5 cut(s) 329, 423, 758, 776, 874
FaqI GGGAC 3 cut(s) 518, 1316, 1406
FatI CATG 5 cut(s) 325, 419, 754, 772, 870
FauI CCCGC 2 cut(s) 1049, 1670
Fnu4HI GCNGC 9 cut(s) 166, 207, 641, 925, 1194, 1571, 1574, 1577, 1580
FokI GGATG 5 cut(s) 402, 752, 995, 1039, 1320
FriOI GRGCYC 1 cut(s) 1670
Fsp4HI GCNGC 9 cut(s) 166, 207, 641, 925, 1194, 1571, 1574, 1577, 1580
FspBI CTAG 4 cut(s) 359, 591, 1016, 1701
GlaI GCGC 3 cut(s) 1478, 1527, 1676
GluI GCNGC 9 cut(s) 166, 207, 641, 925, 1194, 1571, 1574, 1577, 1580
GsuI CTGGAG 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 1529
HaeIII GGCC 5 cut(s) 165, 478, 1653, 1668, 1724
HapII CCGG 2 cut(s) 162, 1725
HgaI GACGC 3 cut(s) 247, 726, 1694
HhaI GCGC 3 cut(s) 1479, 1528, 1677
Hin1I GRCGYC 3 cut(s) 258, 1526, 1705
Hin1II CATG 5 cut(s) 329, 423, 758, 776, 874
Hin6I GCGC 3 cut(s) 1477, 1526, 1675
HinP1I GCGC 3 cut(s) 1477, 1526, 1675
HincII GTYRAC 2 cut(s) 283, 748
HindII GTYRAC 2 cut(s) 283, 748
HindIII AAGCTT 4 cut(s) 894, 1010, 1067, 1277
HinfI GANTC 6 cut(s) 22, 542, 653, 671, 1003, 1742
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 162, 1725
HphI GGTGA 3 cut(s) 254, 898, 1700
Hpy166II GTNNAC 5 cut(s) 283, 685, 710, 748, 1170
Hpy188I TCNGA 5 cut(s) 541, 616, 1270, 1418, 1494
Hpy188III TCNNGA 5 cut(s) 591, 912, 1100, 1228, 1301
Hpy8I GTNNAC 5 cut(s) 283, 685, 710, 748, 1170
HpyCH4III ACNGT 5 cut(s) 689, 859, 955, 1414, 1469
HpyCH4IV ACGT 6 cut(s) 100, 231, 345, 1156, 1164, 1329
HpyCH4V TGCA 9 cut(s) 98, 153, 319, 640, 685, 788, 964, 1021, 1136
HpyF10VI GCNNNNNNNGC 7 cut(s) 364, 1018, 1190, 1525, 1576, 1579, 1674
HpyF3I CTNAG 3 cut(s) 613, 1008, 1179
HpySE526I ACGT 6 cut(s) 100, 231, 345, 1156, 1164, 1329
Hsp92I GRCGYC 3 cut(s) 258, 1526, 1705
Hsp92II CATG 5 cut(s) 329, 423, 758, 776, 874
HspAI GCGC 3 cut(s) 1477, 1526, 1675
KasI GGCGCC 1 cut(s) 1525
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 283
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 5 cut(s) 147, 311, 1084, 1375, 1684
LmnI GCTCC 3 cut(s) 763, 1324, 1516
Lsp1109I GCAGC 3 cut(s) 652, 936, 1205
LweI GCATC 5 cut(s) 105, 300, 424, 513, 797
MaeI CTAG 4 cut(s) 359, 591, 1016, 1701
MaeII ACGT 6 cut(s) 100, 231, 345, 1156, 1164, 1329
MaeIII GTNAC 9 cut(s) 338, 346, 395, 491, 886, 1126, 1595, 1706, 1727
MalI GATC 5 cut(s) 149, 313, 1086, 1377, 1686
MboI GATC 5 cut(s) 147, 311, 1084, 1375, 1684
MboII GAAGA 3 cut(s) 257, 590, 1012
MflI RGATCY 3 cut(s) 147, 311, 1084
MhlI GDGCHC 4 cut(s) 687, 1329, 1521, 1670
MluCI AATT 8 cut(s) 559, 583, 602, 805, 1095, 1137, 1398, 1480
Mly113I GGCGCC 1 cut(s) 1526
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 2 cut(s) 157, 214
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 4 cut(s) 282, 555, 563, 1750
MslI CAYNNNNRTG 1 cut(s) 1506
MspA1I CMGCKG 2 cut(s) 209, 1196
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 2 cut(s) 162, 1725
MspR9I CCNGG 5 cut(s) 411, 714, 1390, 1523, 1656
Mva1269I GAATGC 1 cut(s) 1216
MvaI CCWGG 5 cut(s) 411, 714, 1390, 1523, 1656
MvnI CGCG 2 cut(s) 209, 1677
MwoI GCNNNNNNNGC 7 cut(s) 364, 1018, 1190, 1525, 1576, 1579, 1674
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 1526
NdeII GATC 5 cut(s) 147, 311, 1084, 1375, 1684
NgoMIV GCCGGC 1 cut(s) 161
NheI GCTAGC 1 cut(s) 358
NlaIII CATG 5 cut(s) 329, 423, 758, 776, 874
NlaIV GGNNCC 7 cut(s) 313, 534, 1086, 1394, 1527, 1667, 1668
NmuCI GTSAC 3 cut(s) 346, 886, 1706
NspI RCATGY 1 cut(s) 874
PciI ACATGT 1 cut(s) 870
PctI GAATGC 1 cut(s) 1216
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 5 cut(s) 22, 542, 653, 1003, 1742
PkrI GCNGC 9 cut(s) 167, 208, 642, 926, 1195, 1572, 1575, 1578, 1581
PleI GAGTC 1 cut(s) 679
PluTI GGCGCC 1 cut(s) 1529
PpsI GAGTC 1 cut(s) 679
Ppu21I YACGTR 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 532
PscI ACATGT 1 cut(s) 870
PsiI TTATAA 3 cut(s) 1152, 1289, 1592
Psp1406I AACGTT 1 cut(s) 1156
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 5 cut(s) 409, 712, 1388, 1521, 1654
PspGI CCWGG 5 cut(s) 409, 712, 1388, 1521, 1654
PspN4I GGNNCC 7 cut(s) 313, 534, 1086, 1394, 1527, 1667, 1668
PspOMI GGGCCC 1 cut(s) 1666
PspPI GGNCC 7 cut(s) 329, 470, 532, 1393, 1652, 1666, 1667
PspPPI RGGWCCY 1 cut(s) 532
PsuI RGATCY 3 cut(s) 147, 311, 1084
PvuII CAGCTG 1 cut(s) 1196
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 1506
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 4 cut(s) 282, 555, 563, 1750
SatI GCNGC 9 cut(s) 166, 207, 641, 925, 1194, 1571, 1574, 1577, 1580
Sau3AI GATC 5 cut(s) 147, 311, 1084, 1375, 1684
Sau96I GGNCC 7 cut(s) 329, 470, 532, 1393, 1652, 1666, 1667
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 5 cut(s) 411, 714, 1390, 1523, 1656
SduI GDGCHC 4 cut(s) 687, 1329, 1521, 1670
SfaNI GCATC 5 cut(s) 105, 300, 424, 513, 797
SfcI CTRYAG 1 cut(s) 59
SfoI GGCGCC 1 cut(s) 1527
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SinI GGWCC 4 cut(s) 329, 470, 532, 1393
SmiMI CAYNNNNRTG 1 cut(s) 1506
SmlI CTYRAG 2 cut(s) 119, 554
SmoI CTYRAG 2 cut(s) 119, 554
Sse9I AATT 8 cut(s) 559, 583, 602, 805, 1095, 1137, 1398, 1480
SspDI GGCGCC 1 cut(s) 1525
SspMI CTAG 4 cut(s) 359, 591, 1016, 1701
StyD4I CCNGG 5 cut(s) 409, 712, 1388, 1521, 1654
StyI CCWWGG 2 cut(s) 849, 1670
TaaI ACNGT 5 cut(s) 689, 859, 955, 1414, 1469
TaiI ACGT 6 cut(s) 103, 234, 348, 1159, 1167, 1332
TaqI TCGA 3 cut(s) 267, 1623, 1687
TasI AATT 8 cut(s) 559, 583, 602, 805, 1095, 1137, 1398, 1480
TauI GCSGC 6 cut(s) 168, 209, 1573, 1576, 1579, 1582
TfiI GAWTC 5 cut(s) 22, 542, 653, 1003, 1742
Tru1I TTAA 4 cut(s) 282, 555, 563, 1750
Tru9I TTAA 4 cut(s) 282, 555, 563, 1750
TscAI CASTG 4 cut(s) 268, 692, 773, 1417
TseFI GTSAC 3 cut(s) 346, 886, 1706
TseI GCWGC 3 cut(s) 640, 924, 1193
Tsp45I GTSAC 3 cut(s) 346, 886, 1706
TspDTI ATGAA 3 cut(s) 264, 591, 1383
TspGWI ACGGA 4 cut(s) 90, 905, 995, 1187
TspRI CASTG 4 cut(s) 268, 692, 773, 1417
Vha464I CTTAAG 1 cut(s) 554
VneI GTGCAC 1 cut(s) 683
VpaK11BI GGWCC 4 cut(s) 329, 470, 532, 1393
XapI RAATTY 5 cut(s) 559, 583, 602, 805, 1095
XbaI TCTAGA 1 cut(s) 590
XceI RCATGY 1 cut(s) 874
XspI CTAG 4 cut(s) 359, 591, 1016, 1701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.