pycom05g29010

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011139
Physical Location & Seq
Forward (+)
29564426 .. 29568610
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29010.9

Sequence Viewer

Length: 1791 bp
ATGACTGGAGGGTCATTGGGGATTCGCTCAGGGAGTTATGGTTCTTTGGAAAAACAGCTACAGAACAACAACATCAACAGCATCAACGGAGTGTTGCCAATCCAATCTGCAAGGAAGCCGTCGAAAATGCTCAAGGAGAAGGATAGGTTGTTCCATTGGATCGATCACTTCATGAGGGTACTGCAAGTTTGCCGGCCGGAAGAAGGTTGGAATGCTGCTGCTCTGCCTCATCTCCGCCGCAGTTTTCGTCTGGGTTTTGTACGTTGGAAAAGAGGTGAAGATGGACAAGAGGTTGTCAATGTCGGAAACATTAGTGTTAACAACAGCATCACTTTAAGTTTTTCTGAATCCTCAAGACAACATGGAGCTCAAAGTATCAACATGGCTTTTCCGCTGGCGTTGCCCCCTCCTCCTCCTGCTGCTTTTCTGGGTTACACTCTTCCTCCTGGGCATCCATGTAATACATTCACTTTGCCTCCCCCACCAGCAGATAAAAAAAGAACTGGACCACGACCTTGTCCAGTATGTTACCTTCCTGTGGAAGAAGCCATAGCCTTGATGCCGAAGGTCCCTTCATATTCTCCGGTTCTTAAGAATTTAACATACATTTATGAAGAGAATTTATCAAGAGATACAGAATTTGGAGGCTCAGATTTTGGTGGATACCCTACATTGAAGCAAAGGAGTGATTCTTATGATATAAGAGAGTCAATGAGTGTGCACTGCGGATTTGTTAAAGGAAGTAAACCTGGGCGGAATACAGGATATGATATGGATGAGGTTGACCTAAATGATATGGAGCAGTGTAATGGAGTGGTTGTTGCATCGGCTATATTTGGAAATTTTGATGAGATAAACCAGCCAAGGAACATTAGTGATTATTCCAAGGAGACCGTTTGCTTCTACATGTTTATAGATAATGTGACAGAAGCTTACTTGAGGACATCTGGAAAAATGGGCAGCGATCGGAAAGTTGGTATATGGAGAACTGTGGTTGTGCATAATCCTCCTTACACAGATGGAAGGCGCACTGGGAAGATTCCCAAGCTTCTAGTGCATAGGATGTTTCCTAATGCCCGCTTTTCTTTATGGATTGATGGGAAACTTGAACTTGTCGTGGATCCATATCAAATTCTTGAAAGGTTCTTGTGGAGAAAGAATGCAACTTTTGCAATTTCTAGACATTATAAACGCTTTGACGTGTTTACGGAAGCTGAGGCAAATAAAGCTGCTGGAAAATACGACAATGCCTCTATTGACTTCCAGGTTGAATTTTACAAAAGGGAAGGTTTGACCCCATATTCTGAGGAAAAGCTTCCCATTACAAGTGACGTTCCTGAAGGATGTGTTATAATAAGGGAGCATGTCCCCATCAGCAACCTCGCTGCTTGTCTTTGGTTCCATGAAGTTGATCGTTTTACTTCCAGGGATCAAATTAGTTTTTGCATAGTGAGAGACCACATTCGGGCAAAGACAAACTGGACTATCAGTATGTTCTACGACTGTGAAAGGCGCAATTTTGTTGTTCAGAAATATCATAGAGATGTATTGGAGCACATGGCTCCTCGTCTTCCTAAGGCTGTTCCTCCACCACCGCTATTACCACCACCACCTCCCTCACTCCCAGCTTTAAGTAACGAACCATCAGTTAAACCATCAACCGAAACTTCACCCGAAAGGGTTGTAAACGCCCCAGCTAAGAGGCCTCCAAGGCGCGGGAGAGATAGGAGGCCGGGCTCTAGGCGTCACCGCAAAGTTGTTGCCGGTGGTAGAGACATTGATTCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

597

Amino Acids

67.44

Weight (kDa)

9.26

Isoelectric Point (pI)

59.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28240
fragaria_vesca FvH4_3g09110 FvH4_3g09110 FvH4_3g09110 FvH4_3g09110
malus_domestica MD05G1338800.v1.1 MD10G1293800.v1.1
prunus_persica Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1
pyrus_communis pycom05g29010 pycom10g24570
rosa_chinensis RchiOBHm_Chr5g0008541
rosa_laevigata RLG00000031578
rosa_multiflora Rmu_sc0001665.1_g000015
rosa_roxburghii Rroxscaffold_1G00068020
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5BG062700 Rh5DG063400
rosa_wichuraiana Rw5G005560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1188, 1352
AasI GACNNNNNNGTC 1 cut(s) 10
AccII CGCG 1 cut(s) 1716
AciI CCGC 9 cut(s) 235, 238, 392, 726, 754, 1078, 1595, 1716, 1750
AclWI GGATC 4 cut(s) 167, 1115, 1128, 1437
AcoI YGGCCR 1 cut(s) 194
AcsI RAATTY 6 cut(s) 595, 619, 638, 841, 1131, 1271
AcuI CTGAAG 1 cut(s) 1359
AcyI GRCGYC 1 cut(s) 1744
AfaI GTAC 2 cut(s) 180, 261
AfiI CCNNNNNNNGG 4 cut(s) 203, 538, 1465, 1715
AflII CTTAAG 1 cut(s) 590
AflIII ACRYGT 2 cut(s) 906, 1200
AgsI TTSAA 4 cut(s) 676, 1109, 1139, 1271
AjiI CACGTC 1 cut(s) 1201
AjnI CCWGG 4 cut(s) 445, 748, 1263, 1424
AloI GAACNNNNNNTCC 4 cut(s) 25, 57, 134, 166
AluBI AGCT 9 cut(s) 58, 368, 932, 1048, 1214, 1229, 1315, 1628, 1697
AluI AGCT 9 cut(s) 58, 368, 932, 1048, 1214, 1229, 1315, 1628, 1697
Alw21I GWGCWC 3 cut(s) 370, 723, 1557
Alw26I GTCTC 3 cut(s) 884, 1449, 1767
Alw44I GTGCAC 1 cut(s) 719
AlwI GGATC 4 cut(s) 167, 1115, 1128, 1437
AoxI GGCC 3 cut(s) 194, 1703, 1730
ApaLI GTGCAC 1 cut(s) 719
ApeKI GCWGC 6 cut(s) 215, 218, 419, 960, 1229, 1385
ApoI RAATTY 6 cut(s) 595, 619, 638, 841, 1131, 1271
Asp700I GAANNNNTTC 1 cut(s) 1314
AspLEI GCGC 3 cut(s) 1029, 1515, 1716
AspS9I GGNCC 2 cut(s) 506, 568
AsuC2I CCSGG 1 cut(s) 1734
AsuHPI GGTGA 3 cut(s) 287, 1662, 1739
AvaII GGWCC 2 cut(s) 506, 568
AxyI CCTNAGG 1 cut(s) 1575
BaeGI GKGCMC 1 cut(s) 723
BamHI GGATCC 1 cut(s) 1120
BanII GRGCYC 2 cut(s) 370, 1739
BarI GAAGNNNNNNTAC 2 cut(s) 516, 548
BbsI GAAGAC 1 cut(s) 1562
Bbv12I GWGCWC 3 cut(s) 370, 723, 1557
BbvCI CCTCAGC 1 cut(s) 1215
BbvI GCAGC 6 cut(s) 202, 205, 406, 972, 1216, 1372
BccI CCATC 6 cut(s) 275, 1013, 1091, 1379, 1651, 1663
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 4 cut(s) 447, 750, 1265, 1426
BciVI GTATCC 1 cut(s) 656
BcnI CCSGG 1 cut(s) 1734
BcoDI GTCTC 3 cut(s) 884, 1449, 1767
BfaI CTAG 3 cut(s) 1052, 1179, 1740
BfmI CTRYAG 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 590
BfuI GTATCC 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 1711
BisI GCNGC 7 cut(s) 216, 219, 238, 420, 961, 1230, 1386
BlsI GCNGC 7 cut(s) 217, 220, 239, 421, 962, 1231, 1387
Bme1390I CCNGG 5 cut(s) 447, 750, 1265, 1426, 1734
Bme18I GGWCC 2 cut(s) 506, 568
BmgBI CACGTC 1 cut(s) 1201
BmgT120I GGNCC 2 cut(s) 506, 568
BmiI GGNNCC 4 cut(s) 570, 1122, 1400, 1563
BmrFI CCNGG 5 cut(s) 447, 750, 1265, 1426, 1734
BmrI ACTGGG 1 cut(s) 1041
BmsI GCATC 5 cut(s) 90, 336, 460, 549, 833
BmuI ACTGGG 1 cut(s) 1041
BpiI GAAGAC 1 cut(s) 1562
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 2 cut(s) 28, 1215
BpuEI CTTGAG 3 cut(s) 116, 337, 958
BpuMI CCSGG 1 cut(s) 1734
Bsa29I ATCGAT 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 1125
BsaHI GRCGYC 1 cut(s) 1744
BsaI GGTCTC 2 cut(s) 884, 1449
BsaJI CCNNGG 6 cut(s) 446, 749, 863, 885, 1425, 1709
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 8 cut(s) 25, 55, 427, 457, 460, 490, 804, 834
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 538, 1465, 1715
Bse118I RCCGGY 2 cut(s) 192, 1763
Bse1I ACTGG 5 cut(s) 10, 508, 521, 1036, 1484
Bse21I CCTNAGG 1 cut(s) 1575
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 4 cut(s) 447, 750, 1265, 1426
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 6 cut(s) 446, 749, 863, 885, 1425, 1709
BseGI GGATG 4 cut(s) 451, 781, 1068, 1349
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 4 cut(s) 203, 538, 1465, 1715
BseMII CTCAG 4 cut(s) 42, 663, 1206, 1296
BseNI ACTGG 5 cut(s) 10, 508, 521, 1036, 1484
BseRI GAGGAG 3 cut(s) 399, 402, 1554
BseSI GKGCMC 1 cut(s) 723
BseX3I CGGCCG 1 cut(s) 194
BseXI GCAGC 6 cut(s) 202, 205, 406, 972, 1216, 1372
BseYI CCCAGC 2 cut(s) 1624, 1693
Bsh1236I CGCG 1 cut(s) 1716
Bsh1285I CGRYCG 2 cut(s) 197, 967
BshFI GGCC 3 cut(s) 196, 1705, 1732
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 2 cut(s) 197, 967
BsiHKAI GWGCWC 3 cut(s) 370, 723, 1557
BsiSI CCGG 5 cut(s) 193, 197, 584, 1733, 1764
BslFI GGGAC 2 cut(s) 554, 1352
BslI CCNNNNNNNGG 4 cut(s) 203, 538, 1465, 1715
BsmAI GTCTC 3 cut(s) 884, 1449, 1767
BsmFI GGGAC 2 cut(s) 554, 1352
BsmI GAATGC 2 cut(s) 217, 1165
BsnI GGCC 3 cut(s) 196, 1705, 1732
Bso31I GGTCTC 2 cut(s) 884, 1449
Bsp1286I GDGCHC 4 cut(s) 370, 723, 1557, 1739
Bsp143I GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
BspACI CCGC 9 cut(s) 235, 238, 392, 726, 754, 1078, 1595, 1716, 1750
BspANI GGCC 3 cut(s) 196, 1705, 1732
BspCNI CTCAG 4 cut(s) 41, 662, 1207, 1297
BspDI ATCGAT 1 cut(s) 162
BspFNI CGCG 1 cut(s) 1716
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 4 cut(s) 570, 1122, 1400, 1563
BspPI GGATC 4 cut(s) 167, 1115, 1128, 1437
BspTI CTTAAG 1 cut(s) 590
BspTNI GGTCTC 2 cut(s) 884, 1449
BsrFI RCCGGY 2 cut(s) 192, 1763
BsrI ACTGG 5 cut(s) 10, 508, 521, 1036, 1484
BssAI RCCGGY 2 cut(s) 192, 1763
BssECI CCNNGG 6 cut(s) 446, 749, 863, 885, 1425, 1709
BssMI GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
BssNI GRCGYC 1 cut(s) 1744
BssT1I CCWWGG 3 cut(s) 863, 885, 1709
Bst2UI CCWGG 4 cut(s) 447, 750, 1265, 1426
Bst4CI ACNGT 3 cut(s) 895, 991, 1505
Bst6I CTCTTC 2 cut(s) 444, 609
BstACI GRCGYC 1 cut(s) 1744
BstAFI CTTAAG 1 cut(s) 590
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstC8I GCNNGC 3 cut(s) 194, 396, 1078
BstDEI CTNAG 6 cut(s) 28, 649, 1215, 1305, 1575, 1698
BstF5I GGATG 4 cut(s) 451, 781, 1068, 1349
BstFNI CGCG 1 cut(s) 1716
BstHHI GCGC 3 cut(s) 1029, 1515, 1716
BstKTI GATC 6 cut(s) 162, 166, 967, 1123, 1414, 1432
BstMAI GTCTC 3 cut(s) 884, 1449, 1767
BstMBI GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
BstMCI CGRYCG 2 cut(s) 197, 967
BstMWI GCNNNNNNNGC 5 cut(s) 400, 1054, 1169, 1226, 1711
BstNI CCWGG 4 cut(s) 447, 750, 1265, 1426
BstNSI RCATGY 2 cut(s) 910, 1367
BstSCI CCNGG 5 cut(s) 445, 748, 1263, 1424, 1732
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 723
BstUI CGCG 1 cut(s) 1716
BstV1I GCAGC 6 cut(s) 202, 205, 406, 972, 1216, 1372
BstV2I GAAGAC 1 cut(s) 1562
BstX2I RGATCY 1 cut(s) 1120
BstYI RGATCY 1 cut(s) 1120
BstZI CGGCCG 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 1575
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 3 cut(s) 196, 1705, 1732
BsuTUI ATCGAT 1 cut(s) 162
BtrI CACGTC 1 cut(s) 1201
BtsCI GGATG 4 cut(s) 451, 781, 1068, 1349
BtsI GCAGTG 2 cut(s) 721, 809
BtsIMutI CAGTG 3 cut(s) 721, 809, 1029
Cac8I GCNNGC 3 cut(s) 194, 396, 1078
CciI TCATGA 1 cut(s) 171
CfoI GCGC 3 cut(s) 1029, 1515, 1716
Cfr10I RCCGGY 2 cut(s) 192, 1763
Cfr13I GGNCC 2 cut(s) 506, 568
ClaI ATCGAT 1 cut(s) 162
CseI GACGC 1 cut(s) 1733
Csp6I GTAC 2 cut(s) 179, 260
CviAII CATG 8 cut(s) 172, 362, 382, 456, 907, 1364, 1403, 1558
CviQI GTAC 2 cut(s) 179, 260
DdeI CTNAG 6 cut(s) 28, 649, 1215, 1305, 1575, 1698
DpnI GATC 6 cut(s) 161, 165, 966, 1122, 1413, 1431
DpnII GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 194
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 2 cut(s) 444, 609
EarI CTCTTC 2 cut(s) 444, 609
EciI GGCGGA 2 cut(s) 224, 769
Ecl136II GAGCTC 1 cut(s) 368
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 3 cut(s) 863, 885, 1709
Eco147I AGGCCT 1 cut(s) 1705
Eco24I GRGCYC 2 cut(s) 370, 1739
Eco31I GGTCTC 2 cut(s) 884, 1449
Eco47I GGWCC 2 cut(s) 506, 568
Eco52I CGGCCG 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 1359
Eco81I CCTNAGG 1 cut(s) 1575
EcoICRI GAGCTC 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 4 cut(s) 445, 748, 1263, 1424
EcoT14I CCWWGG 3 cut(s) 863, 885, 1709
EcoT38I GRGCYC 2 cut(s) 370, 1739
ErhI CCWWGG 3 cut(s) 863, 885, 1709
FaeI CATG 8 cut(s) 175, 365, 385, 459, 910, 1367, 1406, 1561
FaqI GGGAC 2 cut(s) 554, 1352
FatI CATG 8 cut(s) 171, 361, 381, 455, 906, 1363, 1402, 1557
FauI CCCGC 2 cut(s) 1085, 1709
Fnu4HI GCNGC 7 cut(s) 216, 219, 238, 420, 961, 1230, 1386
FokI GGATG 4 cut(s) 438, 788, 1075, 1356
FriOI GRGCYC 2 cut(s) 370, 1739
Fsp4HI GCNGC 7 cut(s) 216, 219, 238, 420, 961, 1230, 1386
FspBI CTAG 3 cut(s) 1052, 1179, 1740
GlaI GCGC 3 cut(s) 1028, 1514, 1715
GluI GCNGC 7 cut(s) 216, 219, 238, 420, 961, 1230, 1386
GsaI CCCAGC 2 cut(s) 1628, 1697
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 3 cut(s) 196, 1705, 1732
HapII CCGG 5 cut(s) 193, 197, 584, 1733, 1764
HgaI GACGC 1 cut(s) 1733
HhaI GCGC 3 cut(s) 1029, 1515, 1716
Hin1I GRCGYC 1 cut(s) 1744
Hin1II CATG 8 cut(s) 175, 365, 385, 459, 910, 1367, 1406, 1561
Hin6I GCGC 3 cut(s) 1027, 1513, 1714
HinP1I GCGC 3 cut(s) 1027, 1513, 1714
HincII GTYRAC 2 cut(s) 319, 784
HindII GTYRAC 2 cut(s) 319, 784
HindIII AAGCTT 3 cut(s) 930, 1046, 1313
HinfI GANTC 6 cut(s) 22, 347, 689, 707, 1039, 1781
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 5 cut(s) 193, 197, 584, 1733, 1764
HphI GGTGA 3 cut(s) 287, 1662, 1739
Hpy166II GTNNAC 6 cut(s) 319, 721, 746, 784, 1206, 1687
Hpy188I TCNGA 6 cut(s) 305, 346, 652, 969, 1306, 1530
Hpy188III TCNNGA 7 cut(s) 172, 354, 627, 948, 1136, 1179, 1337
Hpy8I GTNNAC 6 cut(s) 319, 721, 746, 784, 1206, 1687
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 8 cut(s) 133, 197, 542, 559, 582, 1017, 1280, 1334
HpyCH4III ACNGT 3 cut(s) 895, 991, 1505
HpyCH4IV ACGT 3 cut(s) 262, 1200, 1332
HpyCH4V TGCA 9 cut(s) 110, 184, 721, 824, 1000, 1057, 1163, 1172, 1446
HpyF10VI GCNNNNNNNGC 5 cut(s) 400, 1054, 1169, 1226, 1711
HpyF3I CTNAG 6 cut(s) 28, 649, 1215, 1305, 1575, 1698
HpySE526I ACGT 3 cut(s) 262, 1200, 1332
Hsp92I GRCGYC 1 cut(s) 1744
Hsp92II CATG 8 cut(s) 175, 365, 385, 459, 910, 1367, 1406, 1561
HspAI GCGC 3 cut(s) 1027, 1513, 1714
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
LmnI GCTCC 5 cut(s) 365, 799, 1360, 1552, 1567
Lsp1109I GCAGC 6 cut(s) 202, 205, 406, 972, 1216, 1372
LweI GCATC 5 cut(s) 90, 336, 460, 549, 833
MaeI CTAG 3 cut(s) 1052, 1179, 1740
MaeII ACGT 3 cut(s) 262, 1200, 1332
MaeIII GTNAC 6 cut(s) 431, 527, 922, 1328, 1634, 1745
MalI GATC 6 cut(s) 161, 165, 966, 1122, 1413, 1431
MboI GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
MboII GAAGA 7 cut(s) 212, 290, 431, 554, 626, 1048, 1562
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 4 cut(s) 370, 723, 1557, 1739
MluCI AATT 9 cut(s) 595, 619, 638, 841, 1131, 1173, 1271, 1434, 1516
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 3 cut(s) 188, 245, 283
MroNI GCCGGC 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 1314
MseI TTAA 8 cut(s) 318, 335, 591, 599, 735, 1631, 1650, 1789
MslI CAYNNNNRTG 1 cut(s) 1542
MspA1I CMGCKG 1 cut(s) 394
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 5 cut(s) 193, 197, 584, 1733, 1764
MspR9I CCNGG 5 cut(s) 447, 750, 1265, 1426, 1734
Mva1269I GAATGC 2 cut(s) 217, 1165
MvaI CCWGG 4 cut(s) 447, 750, 1265, 1426
MvnI CGCG 1 cut(s) 1716
MwoI GCNNNNNNNGC 5 cut(s) 400, 1054, 1169, 1226, 1711
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 1 cut(s) 1734
NdeII GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 8 cut(s) 175, 365, 385, 459, 910, 1367, 1406, 1561
NlaIV GGNNCC 4 cut(s) 570, 1122, 1400, 1563
NmuCI GTSAC 3 cut(s) 922, 1328, 1745
NspI RCATGY 2 cut(s) 910, 1367
PagI TCATGA 1 cut(s) 171
PceI AGGCCT 1 cut(s) 1705
PciI ACATGT 1 cut(s) 906
PctI GAATGC 2 cut(s) 217, 1165
PdiI GCCGGC 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 1314
PfeI GAWTC 5 cut(s) 22, 347, 689, 1039, 1781
PflFI GACNNNGTC 1 cut(s) 516
PkrI GCNGC 7 cut(s) 217, 220, 239, 421, 962, 1231, 1387
Ple19I CGATCG 1 cut(s) 967
PleI GAGTC 1 cut(s) 715
PpsI GAGTC 1 cut(s) 715
PpuMI RGGWCCY 1 cut(s) 568
PscI ACATGT 1 cut(s) 906
PsiI TTATAA 2 cut(s) 1188, 1352
Psp124BI GAGCTC 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 4 cut(s) 445, 748, 1263, 1424
PspFI CCCAGC 2 cut(s) 1624, 1693
PspGI CCWGG 4 cut(s) 445, 748, 1263, 1424
PspN4I GGNNCC 4 cut(s) 570, 1122, 1400, 1563
PspPI GGNCC 2 cut(s) 506, 568
PspPPI RGGWCCY 1 cut(s) 568
PsuI RGATCY 1 cut(s) 1120
PsyI GACNNNGTC 1 cut(s) 516
PvuI CGATCG 1 cut(s) 967
RsaI GTAC 2 cut(s) 180, 261
RsaNI GTAC 2 cut(s) 179, 260
RseI CAYNNNNRTG 1 cut(s) 1542
SacI GAGCTC 1 cut(s) 370
SaqAI TTAA 8 cut(s) 318, 335, 591, 599, 735, 1631, 1650, 1789
SatI GCNGC 7 cut(s) 216, 219, 238, 420, 961, 1230, 1386
Sau3AI GATC 6 cut(s) 159, 163, 964, 1120, 1411, 1429
Sau96I GGNCC 2 cut(s) 506, 568
SchI GAGTC 1 cut(s) 716
ScrFI CCNGG 5 cut(s) 447, 750, 1265, 1426, 1734
SduI GDGCHC 4 cut(s) 370, 723, 1557, 1739
SfaNI GCATC 5 cut(s) 90, 336, 460, 549, 833
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 2 cut(s) 506, 568
SmiMI CAYNNNNRTG 1 cut(s) 1542
SmlI CTYRAG 4 cut(s) 131, 352, 590, 937
SmoI CTYRAG 4 cut(s) 131, 352, 590, 937
Sse9I AATT 9 cut(s) 595, 619, 638, 841, 1131, 1173, 1271, 1434, 1516
SseBI AGGCCT 1 cut(s) 1705
SsiI CCGC 9 cut(s) 235, 238, 392, 726, 754, 1078, 1595, 1716, 1750
SspMI CTAG 3 cut(s) 1052, 1179, 1740
SstI GAGCTC 1 cut(s) 370
StuI AGGCCT 1 cut(s) 1705
StyD4I CCNGG 5 cut(s) 445, 748, 1263, 1424, 1732
StyI CCWWGG 3 cut(s) 863, 885, 1709
TaaI ACNGT 3 cut(s) 895, 991, 1505
TaiI ACGT 3 cut(s) 265, 1203, 1335
TaqI TCGA 2 cut(s) 122, 162
TasI AATT 9 cut(s) 595, 619, 638, 841, 1131, 1173, 1271, 1434, 1516
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 5 cut(s) 22, 347, 689, 1039, 1781
Tru1I TTAA 8 cut(s) 318, 335, 591, 599, 735, 1631, 1650, 1789
Tru9I TTAA 8 cut(s) 318, 335, 591, 599, 735, 1631, 1650, 1789
TscAI CASTG 3 cut(s) 728, 809, 1036
TseFI GTSAC 3 cut(s) 922, 1328, 1745
TseI GCWGC 6 cut(s) 215, 218, 419, 960, 1229, 1385
Tsp45I GTSAC 3 cut(s) 922, 1328, 1745
TspDTI ATGAA 4 cut(s) 160, 564, 627, 1419
TspGWI ACGGA 2 cut(s) 102, 1223
TspRI CASTG 3 cut(s) 728, 809, 1036
Tth111I GACNNNGTC 1 cut(s) 516
Vha464I CTTAAG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 719
VpaK11BI GGWCC 2 cut(s) 506, 568
XapI RAATTY 6 cut(s) 595, 619, 638, 841, 1131, 1271
XbaI TCTAGA 1 cut(s) 1178
XceI RCATGY 2 cut(s) 910, 1367
XmnI GAANNNNTTC 1 cut(s) 1314
XspI CTAG 3 cut(s) 1052, 1179, 1740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.