MD10G1293800.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011139
Physical Location & Seq
Forward (+)
38205515 .. 38210460
4946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1293800.v1.1.491

Sequence Viewer

Length: 1767 bp
ATGACTGGAGGGTCATTGGGGATTCGATCAGGGAGTTATGGTTCTTTGGATAAACAGCTACACAACAACATCAACAGCATCAACGGAGTGTTGCCAATCCAATCTGCGCGTAAGCCTTCGAAAATGCTCAAGGAGAAGGAGAGGTTGTTCCACTGGATCTGCAAGTTTGCCGGCCGGAAGAAGGTTGGAATGCTGCTGCTCTGCCTCATCTCCGCAGCGGTTTTTGTCTGGGTTTTGTACGTTGGAAAAGGTGAAGACGGACAAGATGTTGTCAGTGTCGGAAACATTAGTGTTAACAACAGCATCACTTTAAGTTTTTCTGAATCCTCGCGAAAACATGGACCTGAAAGTAACAACATGACTTTTTCGCTGGCGTTGCCTCCTCCTCCTCCTGCTGTTTTTCTGGGTTACACTCTTCCTCCTGGACATCCATGTAATACATTCACTTTGCCTCCCCCACCAGCAGATAAAAAAAGAACTGGACCACGACCTTGTCCAGTATGTTACCTTCCTGTGGAAGAAGCCATAGCCTTGATGCCCAAGGTCCCTTCATATTCTCCAGTTCTGAAGAATTTAACATACATTTATGAAGAAAATTTATCTAGAAAAACAGAATTTGGAGGCTCAGAGTTTGGTGGATACCCTACATTGAAGCAAAGGAGTGATTCATATGATATAAGAGAGTCAATGAGTGTGCATTGTGGATTTGTTAGAGGAAGTAAACCTGGCCGGAATACTGGATATGATCTGGATGAGATTGACCTAAATGATATGGAGCAGTGTAATGGTGTGGTTGTTGCATCGGCTATATTTGGAAATTTTGATGAGATAAACCAGCCAAGGAACATTAGTGATTATTCCAAGGAGACCGTTTGCTTCTACATGTTTATAGATGATGTGACAGAAGCTTACTTGAGAACATCTGGGAAAATGGGCGGCGATAGGAAAGTTGGTATATGGAGAACTGTGGTTGTGCATAACCCTCCTTACACGGATGGAAGGCGCACTGGGAAGATTCCTAAGCTTTTAGTGCATAGGATGTTTCCTAATGCCCGTTTTTCCTTATGGATTGATGGGAAACTTGAACTTGTCGTGGATCCGTATCAAATTCTTGAAAGGTTCTTGTGGAGAAAGAATGCAACTTTTGCGATTTCTAGACATTATAAACGCTTTGACGTGTTTACTGAAGCTGAGGCAAATAAAGCTGCTGGAAAATACGACAATGCCTCTATTGACTTTCAGGTTGAATTTTACAAAAAGGAAGGTTTGACCCCATATTCTGAGGAAAAGCTTCCCATTACAAGTGACGTTCCTGAAGGATGTGTTATAATAAGGGAGCATGTCCCTATCAGCAACCTTGCCGCTTGTCTTTGGTTCCATGAAGTTGATCGTTTTACTTCCAGGGATCAGATTAGTTTTTCCACAGTGAGAGACCACATTCGCGAAAAGACAAACTGGACTATCAATATGTTCTACGACTGTGAAAGACGCAATTTTGTTGTTCAGAAATATCATAGAGATGTATTGGAGCACATGGCTCCTCCTCTTCCTAAGGCTGTTCCTCCACCACCGCTATTACCACCACCACCACCGTCACCCCCAGCTTTAAGTAACGAACCACCAGTTAAACCATCAACCGAAACTTCACCCGAGAGCATTGTAAAAGCCCCAGGTAAGAGGCCTCCAAGGCGTGGGAGAGATAGAAGGTCGGGCTCTAGACGTCACCGCAAAATCGTGGCCGGTGCTAGAGACATCGATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

589

Amino Acids

66.13

Weight (kDa)

9.17

Isoelectric Point (pI)

56.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 192 - 505 1.9e-148 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28240
fragaria_vesca FvH4_3g09110 FvH4_3g09110 FvH4_3g09110 FvH4_3g09110
malus_domestica MD05G1338800.v1.1 MD10G1293800.v1.1
prunus_persica Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1
pyrus_communis pycom05g29010 pycom10g24570
rosa_chinensis RchiOBHm_Chr5g0008541
rosa_laevigata RLG00000031578
rosa_multiflora Rmu_sc0001665.1_g000015
rosa_roxburghii Rroxscaffold_1G00068020
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5BG062700 Rh5DG063400
rosa_wichuraiana Rw5G005560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1164, 1328
AasI GACNNNNNNGTC 1 cut(s) 10
AatII GACGTC 1 cut(s) 1723
AccB7I CCANNNNNTGG 1 cut(s) 1691
AccII CGCG 3 cut(s) 109, 331, 1443
AciI CCGC 6 cut(s) 213, 218, 936, 1362, 1571, 1726
AclWI GGATC 4 cut(s) 164, 1091, 1104, 1413
AcoI YGGCCR 3 cut(s) 172, 727, 1737
AcsI RAATTY 6 cut(s) 571, 595, 614, 817, 1107, 1247
AcuI CTGAAG 3 cut(s) 587, 1206, 1335
AcyI GRCGYC 1 cut(s) 1720
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 3 cut(s) 181, 514, 1691
AflIII ACRYGT 2 cut(s) 882, 1176
AgsI TTSAA 5 cut(s) 652, 1085, 1115, 1247, 1761
AjiI CACGTC 1 cut(s) 1177
AjnI CCWGG 4 cut(s) 421, 724, 1400, 1669
AloI GAACNNNNNNTCC 4 cut(s) 25, 57, 131, 163
AluBI AGCT 7 cut(s) 58, 908, 1024, 1190, 1205, 1291, 1604
AluI AGCT 7 cut(s) 58, 908, 1024, 1190, 1205, 1291, 1604
Alw21I GWGCWC 1 cut(s) 1533
Alw26I GTCTC 3 cut(s) 860, 1425, 1743
AlwI GGATC 4 cut(s) 164, 1091, 1104, 1413
Ama87I CYCGRG 1 cut(s) 1649
AoxI GGCC 4 cut(s) 172, 727, 1679, 1737
ApeKI GCWGC 4 cut(s) 193, 196, 215, 1205
ApoI RAATTY 6 cut(s) 571, 595, 614, 817, 1107, 1247
Asp700I GAANNNNTTC 1 cut(s) 1290
AspLEI GCGC 2 cut(s) 109, 1005
AspS9I GGNCC 3 cut(s) 341, 482, 544
AsuHPI GGTGA 4 cut(s) 263, 1587, 1638, 1715
AsuII TTCGAA 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 1649
AvaII GGWCC 3 cut(s) 341, 482, 544
AxyI CCTNAGG 1 cut(s) 1551
BamHI GGATCC 1 cut(s) 1096
BanII GRGCYC 1 cut(s) 1715
BarI GAAGNNNNNNTAC 2 cut(s) 492, 524
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 1533
BbvCI CCTCAGC 1 cut(s) 1191
BbvI GCAGC 4 cut(s) 180, 183, 227, 1192
BccI CCATC 3 cut(s) 989, 1067, 1639
BciT130I CCWGG 4 cut(s) 423, 726, 1402, 1671
BciVI GTATCC 1 cut(s) 632
BcoDI GTCTC 3 cut(s) 860, 1425, 1743
BfaI CTAG 4 cut(s) 603, 1155, 1716, 1746
BfuI GTATCC 1 cut(s) 632
BglI GCCNNNNNGGC 1 cut(s) 1687
BisI GCNGC 6 cut(s) 194, 197, 216, 937, 1206, 1362
BlsI GCNGC 6 cut(s) 195, 198, 217, 938, 1207, 1363
Bme1390I CCNGG 4 cut(s) 423, 726, 1402, 1671
Bme18I GGWCC 3 cut(s) 341, 482, 544
BmeT110I CYCGRG 1 cut(s) 1649
BmgBI CACGTC 1 cut(s) 1177
BmgT120I GGNCC 3 cut(s) 341, 482, 544
BmiI GGNNCC 4 cut(s) 546, 1098, 1376, 1539
BmrFI CCNGG 4 cut(s) 423, 726, 1402, 1671
BmrI ACTGGG 1 cut(s) 1017
BmsI GCATC 4 cut(s) 87, 312, 525, 809
BmuI ACTGGG 1 cut(s) 1017
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 2 cut(s) 27, 543
Bpu10I CCTNAGC 2 cut(s) 1020, 1191
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 113, 934
Bsa29I ATCGAT 1 cut(s) 1755
BsaBI GATNNNNATC 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 1720
BsaI GGTCTC 2 cut(s) 860, 1425
BsaJI CCNNGG 6 cut(s) 540, 839, 861, 1401, 1669, 1685
BsaXI ACNNNNNCTCC 8 cut(s) 25, 55, 131, 161, 403, 433, 436, 466
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 514, 1691
Bse118I RCCGGY 2 cut(s) 170, 1739
Bse1I ACTGG 9 cut(s) 10, 158, 484, 497, 560, 742, 1012, 1460, 1622
Bse21I CCTNAGG 1 cut(s) 1551
Bse8I GATNNNNATC 1 cut(s) 1101
BseBI CCWGG 4 cut(s) 423, 726, 1402, 1671
BseCI ATCGAT 1 cut(s) 1755
BseDI CCNNGG 6 cut(s) 540, 839, 861, 1401, 1669, 1685
BseGI GGATG 5 cut(s) 427, 757, 1000, 1044, 1325
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 3 cut(s) 181, 514, 1691
BseMII CTCAG 3 cut(s) 639, 1182, 1272
BseNI ACTGG 9 cut(s) 10, 158, 484, 497, 560, 742, 1012, 1460, 1622
BseRI GAGGAG 5 cut(s) 372, 375, 378, 1530, 1533
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 4 cut(s) 180, 183, 227, 1192
BseYI CCCAGC 1 cut(s) 1600
Bsh1236I CGCG 3 cut(s) 109, 331, 1443
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 4 cut(s) 174, 729, 1681, 1739
BshVI ATCGAT 1 cut(s) 1755
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 1533
BsiHKCI CYCGRG 1 cut(s) 1649
BsiSI CCGG 4 cut(s) 171, 175, 730, 1740
BslFI GGGAC 2 cut(s) 530, 1328
BslI CCNNNNNNNGG 3 cut(s) 181, 514, 1691
BsmAI GTCTC 3 cut(s) 860, 1425, 1743
BsmFI GGGAC 2 cut(s) 530, 1328
BsmI GAATGC 2 cut(s) 195, 1141
BsnI GGCC 4 cut(s) 174, 729, 1681, 1739
Bso31I GGTCTC 2 cut(s) 860, 1425
BsoBI CYCGRG 1 cut(s) 1649
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 1533, 1715
Bsp143I GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
Bsp68I TCGCGA 2 cut(s) 331, 1443
BspACI CCGC 6 cut(s) 213, 218, 936, 1362, 1571, 1726
BspANI GGCC 4 cut(s) 174, 729, 1681, 1739
BspCNI CTCAG 3 cut(s) 638, 1183, 1273
BspDI ATCGAT 1 cut(s) 1755
BspFNI CGCG 3 cut(s) 109, 331, 1443
BspLI GGNNCC 4 cut(s) 546, 1098, 1376, 1539
BspPI GGATC 4 cut(s) 164, 1091, 1104, 1413
BspT104I TTCGAA 1 cut(s) 119
BspTNI GGTCTC 2 cut(s) 860, 1425
BsrFI RCCGGY 2 cut(s) 170, 1739
BsrI ACTGG 9 cut(s) 10, 158, 484, 497, 560, 742, 1012, 1460, 1622
BssAI RCCGGY 2 cut(s) 170, 1739
BssECI CCNNGG 6 cut(s) 540, 839, 861, 1401, 1669, 1685
BssMI GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
BssNI GRCGYC 1 cut(s) 1720
BssT1I CCWWGG 4 cut(s) 540, 839, 861, 1685
Bst2UI CCWGG 4 cut(s) 423, 726, 1402, 1671
Bst4CI ACNGT 5 cut(s) 871, 967, 1426, 1481, 1593
Bst6I CTCTTC 2 cut(s) 420, 1551
BstACI GRCGYC 1 cut(s) 1720
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 172, 372
BstDEI CTNAG 5 cut(s) 625, 1020, 1191, 1281, 1551
BstF5I GGATG 5 cut(s) 427, 757, 1000, 1044, 1325
BstFNI CGCG 3 cut(s) 109, 331, 1443
BstHHI GCGC 2 cut(s) 109, 1005
BstKTI GATC 6 cut(s) 29, 159, 748, 1099, 1390, 1408
BstMAI GTCTC 3 cut(s) 860, 1425, 1743
BstMBI GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 5 cut(s) 376, 1030, 1145, 1202, 1687
BstNI CCWGG 4 cut(s) 423, 726, 1402, 1671
BstNSI RCATGY 2 cut(s) 886, 1343
BstSCI CCNGG 4 cut(s) 421, 724, 1400, 1669
BstUI CGCG 3 cut(s) 109, 331, 1443
BstV1I GCAGC 4 cut(s) 180, 183, 227, 1192
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 2 cut(s) 156, 1096
BstYI RGATCY 2 cut(s) 156, 1096
BstZI CGGCCG 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 1755
Bsu36I CCTNAGG 1 cut(s) 1551
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 4 cut(s) 174, 729, 1681, 1739
BsuTUI ATCGAT 1 cut(s) 1755
BtrI CACGTC 1 cut(s) 1177
BtsCI GGATG 5 cut(s) 427, 757, 1000, 1044, 1325
BtsI GCAGTG 1 cut(s) 785
BtsIMutI CAGTG 5 cut(s) 151, 280, 785, 1005, 1431
BtuMI TCGCGA 2 cut(s) 331, 1443
Cac8I GCNNGC 2 cut(s) 172, 372
CfoI GCGC 2 cut(s) 109, 1005
Cfr10I RCCGGY 2 cut(s) 170, 1739
Cfr13I GGNCC 3 cut(s) 341, 482, 544
ClaI ATCGAT 1 cut(s) 1755
CseI GACGC 1 cut(s) 1497
Csp6I GTAC 1 cut(s) 238
CviAII CATG 7 cut(s) 338, 358, 432, 883, 1340, 1379, 1534
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 5 cut(s) 625, 1020, 1191, 1281, 1551
DpnI GATC 6 cut(s) 28, 158, 747, 1098, 1389, 1407
DpnII GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 3 cut(s) 172, 727, 1737
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 420, 1551
EarI CTCTTC 2 cut(s) 420, 1551
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 4 cut(s) 540, 839, 861, 1685
Eco147I AGGCCT 1 cut(s) 1681
Eco24I GRGCYC 1 cut(s) 1715
Eco31I GGTCTC 2 cut(s) 860, 1425
Eco47I GGWCC 3 cut(s) 341, 482, 544
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 3 cut(s) 587, 1206, 1335
Eco81I CCTNAGG 1 cut(s) 1551
Eco88I CYCGRG 1 cut(s) 1649
EcoO109I RGGNCCY 1 cut(s) 544
EcoRII CCWGG 4 cut(s) 421, 724, 1400, 1669
EcoT14I CCWWGG 4 cut(s) 540, 839, 861, 1685
EcoT38I GRGCYC 1 cut(s) 1715
ErhI CCWWGG 4 cut(s) 540, 839, 861, 1685
FaeI CATG 7 cut(s) 341, 361, 435, 886, 1343, 1382, 1537
FaqI GGGAC 2 cut(s) 530, 1328
FatI CATG 7 cut(s) 337, 357, 431, 882, 1339, 1378, 1533
FauNDI CATATG 1 cut(s) 670
Fnu4HI GCNGC 6 cut(s) 194, 197, 216, 937, 1206, 1362
FokI GGATG 5 cut(s) 414, 764, 1007, 1051, 1332
FriOI GRGCYC 1 cut(s) 1715
Fsp4HI GCNGC 6 cut(s) 194, 197, 216, 937, 1206, 1362
FspBI CTAG 4 cut(s) 603, 1155, 1716, 1746
GlaI GCGC 2 cut(s) 108, 1004
GluI GCNGC 6 cut(s) 194, 197, 216, 937, 1206, 1362
GsaI CCCAGC 1 cut(s) 1604
GsuI CTGGAG 2 cut(s) 27, 543
HaeIII GGCC 4 cut(s) 174, 729, 1681, 1739
HapII CCGG 4 cut(s) 171, 175, 730, 1740
HgaI GACGC 1 cut(s) 1497
HhaI GCGC 2 cut(s) 109, 1005
Hin1I GRCGYC 1 cut(s) 1720
Hin1II CATG 7 cut(s) 341, 361, 435, 886, 1343, 1382, 1537
Hin6I GCGC 2 cut(s) 107, 1003
HinP1I GCGC 2 cut(s) 107, 1003
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 3 cut(s) 906, 1022, 1289
HinfI GANTC 6 cut(s) 22, 323, 665, 683, 1015, 1757
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 4 cut(s) 171, 175, 730, 1740
HphI GGTGA 4 cut(s) 263, 1587, 1638, 1715
Hpy166II GTNNAC 3 cut(s) 295, 722, 1182
Hpy188I TCNGA 7 cut(s) 281, 322, 567, 628, 1282, 1410, 1506
Hpy188III TCNNGA 8 cut(s) 330, 603, 749, 1112, 1155, 1313, 1442, 1716
Hpy8I GTNNAC 3 cut(s) 295, 722, 1182
HpyAV CCTTC 9 cut(s) 126, 130, 175, 518, 558, 993, 1256, 1310, 1698
HpyCH4III ACNGT 5 cut(s) 871, 967, 1426, 1481, 1593
HpyCH4IV ACGT 4 cut(s) 240, 1176, 1308, 1720
HpyCH4V TGCA 6 cut(s) 162, 697, 800, 976, 1033, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 376, 1030, 1145, 1202, 1687
HpyF3I CTNAG 5 cut(s) 625, 1020, 1191, 1281, 1551
HpySE526I ACGT 4 cut(s) 240, 1176, 1308, 1720
Hsp92I GRCGYC 1 cut(s) 1720
Hsp92II CATG 7 cut(s) 341, 361, 435, 886, 1343, 1382, 1537
HspAI GCGC 2 cut(s) 107, 1003
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
LmnI GCTCC 4 cut(s) 775, 1336, 1528, 1543
Lsp1109I GCAGC 4 cut(s) 180, 183, 227, 1192
LweI GCATC 4 cut(s) 87, 312, 525, 809
MaeI CTAG 4 cut(s) 603, 1155, 1716, 1746
MaeII ACGT 4 cut(s) 240, 1176, 1308, 1720
MaeIII GTNAC 8 cut(s) 350, 407, 503, 898, 1304, 1593, 1610, 1721
MalI GATC 6 cut(s) 28, 158, 747, 1098, 1389, 1407
MboI GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
MboII GAAGA 8 cut(s) 190, 266, 407, 530, 580, 602, 1024, 1538
MflI RGATCY 2 cut(s) 156, 1096
MhlI GDGCHC 2 cut(s) 1533, 1715
MluCI AATT 7 cut(s) 571, 595, 614, 817, 1107, 1247, 1492
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 3 cut(s) 166, 223, 259
MroNI GCCGGC 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 1290
MseI TTAA 6 cut(s) 294, 311, 575, 1607, 1626, 1765
MslI CAYNNNNRTG 1 cut(s) 1518
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 4 cut(s) 171, 175, 730, 1740
MspR9I CCNGG 4 cut(s) 423, 726, 1402, 1671
Mva1269I GAATGC 2 cut(s) 195, 1141
MvaI CCWGG 4 cut(s) 423, 726, 1402, 1671
MvnI CGCG 3 cut(s) 109, 331, 1443
MwoI GCNNNNNNNGC 5 cut(s) 376, 1030, 1145, 1202, 1687
NaeI GCCGGC 1 cut(s) 172
NdeI CATATG 1 cut(s) 670
NdeII GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 7 cut(s) 341, 361, 435, 886, 1343, 1382, 1537
NlaIV GGNNCC 4 cut(s) 546, 1098, 1376, 1539
NmuCI GTSAC 4 cut(s) 898, 1304, 1593, 1721
NruI TCGCGA 2 cut(s) 331, 1443
NspI RCATGY 2 cut(s) 886, 1343
NspV TTCGAA 1 cut(s) 119
PceI AGGCCT 1 cut(s) 1681
PciI ACATGT 1 cut(s) 882
PctI GAATGC 2 cut(s) 195, 1141
PdiI GCCGGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 1290
PfeI GAWTC 5 cut(s) 22, 323, 665, 1015, 1757
PflFI GACNNNGTC 1 cut(s) 492
PflMI CCANNNNNTGG 1 cut(s) 1691
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 6 cut(s) 195, 198, 217, 938, 1207, 1363
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PpuMI RGGWCCY 1 cut(s) 544
PscI ACATGT 1 cut(s) 882
PsiI TTATAA 2 cut(s) 1164, 1328
Psp5II RGGWCCY 1 cut(s) 544
Psp6I CCWGG 4 cut(s) 421, 724, 1400, 1669
PspFI CCCAGC 1 cut(s) 1600
PspGI CCWGG 4 cut(s) 421, 724, 1400, 1669
PspN4I GGNNCC 4 cut(s) 546, 1098, 1376, 1539
PspPI GGNCC 3 cut(s) 341, 482, 544
PspPPI RGGWCCY 1 cut(s) 544
PsuI RGATCY 2 cut(s) 156, 1096
PsyI GACNNNGTC 1 cut(s) 492
RruI TCGCGA 2 cut(s) 331, 1443
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 1518
SaqAI TTAA 6 cut(s) 294, 311, 575, 1607, 1626, 1765
SatI GCNGC 6 cut(s) 194, 197, 216, 937, 1206, 1362
Sau3AI GATC 6 cut(s) 26, 156, 745, 1096, 1387, 1405
Sau96I GGNCC 3 cut(s) 341, 482, 544
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 4 cut(s) 423, 726, 1402, 1671
SduI GDGCHC 2 cut(s) 1533, 1715
SfaNI GCATC 4 cut(s) 87, 312, 525, 809
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 3 cut(s) 341, 482, 544
SmiMI CAYNNNNRTG 1 cut(s) 1518
SmlI CTYRAG 2 cut(s) 128, 913
SmoI CTYRAG 2 cut(s) 128, 913
Sse9I AATT 7 cut(s) 571, 595, 614, 817, 1107, 1247, 1492
SseBI AGGCCT 1 cut(s) 1681
SsiI CCGC 6 cut(s) 213, 218, 936, 1362, 1571, 1726
SspMI CTAG 4 cut(s) 603, 1155, 1716, 1746
StuI AGGCCT 1 cut(s) 1681
StyD4I CCNGG 4 cut(s) 421, 724, 1400, 1669
StyI CCWWGG 4 cut(s) 540, 839, 861, 1685
TaaI ACNGT 5 cut(s) 871, 967, 1426, 1481, 1593
TaiI ACGT 4 cut(s) 243, 1179, 1311, 1723
TaqI TCGA 3 cut(s) 25, 119, 1755
TasI AATT 7 cut(s) 571, 595, 614, 817, 1107, 1247, 1492
TauI GCSGC 2 cut(s) 939, 1364
TfiI GAWTC 5 cut(s) 22, 323, 665, 1015, 1757
Tru1I TTAA 6 cut(s) 294, 311, 575, 1607, 1626, 1765
Tru9I TTAA 6 cut(s) 294, 311, 575, 1607, 1626, 1765
TscAI CASTG 5 cut(s) 158, 280, 785, 1012, 1431
TseFI GTSAC 4 cut(s) 898, 1304, 1593, 1721
TseI GCWGC 4 cut(s) 193, 196, 215, 1205
Tsp45I GTSAC 4 cut(s) 898, 1304, 1593, 1721
TspDTI ATGAA 4 cut(s) 540, 603, 657, 1395
TspGWI ACGGA 4 cut(s) 99, 273, 1007, 1089
TspRI CASTG 5 cut(s) 158, 280, 785, 1012, 1431
Tth111I GACNNNGTC 1 cut(s) 492
Van91I CCANNNNNTGG 1 cut(s) 1691
VpaK11BI GGWCC 3 cut(s) 341, 482, 544
XapI RAATTY 6 cut(s) 571, 595, 614, 817, 1107, 1247
XbaI TCTAGA 3 cut(s) 602, 1154, 1715
XceI RCATGY 2 cut(s) 886, 1343
XmnI GAANNNNTTC 1 cut(s) 1290
XspI CTAG 4 cut(s) 603, 1155, 1716, 1746
ZraI GACGTC 1 cut(s) 1721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.