RLG00000031578

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011139
Physical Location & Seq
Reverse (-)
6352632 .. 6356936
4305 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031578

Sequence Viewer

Length: 1752 bp
ATGACTACAGGGTCATTGGGAATTCGTTCAGGGAGTTATGGGTCTCTGGATAAACAGGCACAGAACAACAGCATCAGCATCATCAACAACGGAGTGTTGCCTATTCAATCTGCACGAAAGCCTTCGAAAATGCTCAAGGAGAAGGAGAGGCTGTTCCATTCGATCTGCAAGTTTGCTGGCCGGAAAAAAGTGGGAATGCTGCTGCTGTGTGTAATCTCCGCTGCGGTTTTCGGATGGGTTTTGTATGTTGGAAAAGGTGAAGACTCACAAGAAGGCATCGCTCGCCAAAACATTAGTCTCAACAACAGCGTCATTTTAAGGAATCCTGAATCCTTGGTGAAAAACGACAAGCAAAGTTACAACATATCACCGCTAGCCTTGCCCCCTCCTCCTCCTGCTTTTTTCTTGGGTTATACTCTTCCTCCTGGGCATCCATGTAATATATTCACGTTGCCTCCCCCACCAGCAGATAAAAAGAGAACTGGACCACGGCCTTGTCCAGTATGTTATCTTCCTGTTGAAGAAGCCATAGCCTTAATGCCAAAGGTCCCTTCATATTCTCCTGTTCTTAAGAATTTGACATACATTTATGAAGAAAGTTTATCAAGAGAAACAGAATTTGGAGGCTCAGATTTTGGCGGATATCCTACTTTGAAGCAAAGGAGTGATTCATATGATATAAGAGAGTCAATGAGTGTGCATTGTGGGTTTGTCAAAGGAAGTAAACCTGGGCGTAATACAGGGTATGATATGGATGAGGATGACCTACATGATATGGAGCAGTGTCATGGTGTGGTTGTTGCATCAGCTATATTTGCAAATTTTGATGAGATAAACCAGCCAAGGAACATTAGCGATTATTCCAGGGAGACTGTTTGCTTCTACATGTTTATAGACGAGGAGACAGAAGCTTATCTGAAGAACAGTAGTAAACTGGACAGCAGAAAGAAAATTGGTGTATGGAGAACTGTTGTTATTCATAACCCACCTTATAAGGATGGAAGGCGCACTGGGAAGATTCCTAAGCTCCTAGTGCACAGAATGTTTCCCAATGCCCGCTTTTCCTTATGGATCGATGGGAAACTGGAGCTTGTTGTGGATCCATACCAAATTCTTGAAAGGTTTTTGTGGAGGAAGAATGCAACTTTTGCAATTTCTAGACATTATAAACGCTTTGATGTGCTTATGGAAGCTGAGGCAAATAAAGTTGCTGGAAAATACGACAATGCCTCTATTGACTTTCAGGTTGAATTTTACAAAAAGGAAGGTTTGACCCCATATTCTGAGGCAAAACTTCCCATTACAAGTGATGTTCCTGAAGGTTGTGTAATAATAAGGGAGCATGTCCCTATCAGCAACCTCTTCACTTGTCTTTGGTTCAATGAAGTTGATCGTTTTACTTCGAGGGACCAAATTAGTTTTTCAACTGTCAGAGATCACATTACGGCAAAGACAAACTGGACTATCAATATGTTCCTTGACTGTGAAAGGCGCAATTTTGTAGTTCAGAAATACCATAAAGATGTATTAGAGCACATGGCTCCTCATATTCCTGTTGCTGCTCTTCCACCACCACTACCGCCATCACTGCCACCACCAGCTTTGAGTAATGAACCACAAGTCAAACCAGTTGAAACGGCGCCTGAAAGGCCAGTAAGGAAGATCCAACCGGGGCGCCGGAGAGAAAGGAGGCCTGGTTCCAGACGTCACCGCAAAGTTATTACCGCAAATAGAGACACTGATACAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

584

Amino Acids

66.13

Weight (kDa)

9.15

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 193 - 506 1.9e-151 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28240
fragaria_vesca FvH4_3g09110 FvH4_3g09110 FvH4_3g09110 FvH4_3g09110
malus_domestica MD05G1338800.v1.1 MD10G1293800.v1.1
prunus_persica Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1 Prupe.4G048000_v2.0.a1
pyrus_communis pycom05g29010 pycom10g24570
rosa_chinensis RchiOBHm_Chr5g0008541
rosa_laevigata RLG00000031578
rosa_multiflora Rmu_sc0001665.1_g000015
rosa_roxburghii Rroxscaffold_1G00068020
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5BG062700 Rh5DG063400
rosa_wichuraiana Rw5G005560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 993, 1167
AasI GACNNNNNNGTC 1 cut(s) 10
AatII GACGTC 1 cut(s) 1708
AccB1I GGYRCC 2 cut(s) 1639, 1674
AciI CCGC 8 cut(s) 219, 224, 371, 639, 1057, 1580, 1711, 1725
AclWI GGATC 4 cut(s) 1079, 1094, 1107, 1657
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 6 cut(s) 21, 574, 617, 820, 1110, 1250
AcuI CTGAAG 2 cut(s) 938, 1338
AcyI GRCGYC 3 cut(s) 1640, 1675, 1705
AflII CTTAAG 1 cut(s) 569
AflIII ACRYGT 1 cut(s) 885
AgsI TTSAA 8 cut(s) 107, 521, 655, 1118, 1250, 1381, 1425, 1634
AjnI CCWGG 4 cut(s) 424, 727, 863, 1693
AjuI GAANNNNNNNTTGG 4 cut(s) 535, 567, 603, 635
AloI GAACNNNNNNTCC 4 cut(s) 137, 169, 1681, 1713
AluBI AGCT 6 cut(s) 809, 911, 1027, 1090, 1193, 1601
AluI AGCT 6 cut(s) 809, 911, 1027, 1090, 1193, 1601
Alw21I GWGCWC 2 cut(s) 1038, 1536
Alw26I GTCTC 5 cut(s) 48, 302, 863, 896, 1728
Alw44I GTGCAC 1 cut(s) 1034
AlwI GGATC 4 cut(s) 1079, 1094, 1107, 1657
AoxI GGCC 4 cut(s) 178, 491, 1649, 1691
ApaLI GTGCAC 1 cut(s) 1034
ApeKI GCWGC 4 cut(s) 199, 202, 221, 1559
ApoI RAATTY 6 cut(s) 21, 574, 617, 820, 1110, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 280, 312
Asp700I GAANNNNTTC 2 cut(s) 25, 121
AspLEI GCGC 4 cut(s) 1008, 1494, 1642, 1677
AspS9I GGNCC 3 cut(s) 485, 547, 1408
AsuC2I CCSGG 1 cut(s) 1671
AsuHPI GGTGA 4 cut(s) 269, 349, 360, 1700
AsuII TTCGAA 1 cut(s) 125
AsuNHI GCTAGC 1 cut(s) 373
AvaII GGWCC 3 cut(s) 485, 547, 1408
BaeGI GKGCMC 1 cut(s) 1038
BamHI GGATCC 1 cut(s) 1099
BanI GGYRCC 2 cut(s) 1639, 1674
BarI GAAGNNNNNNTAC 2 cut(s) 495, 527
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 1038, 1536
BbvCI CCTCAGC 1 cut(s) 1194
BbvI GCAGC 4 cut(s) 186, 189, 208, 1546
BccI CCATC 4 cut(s) 228, 992, 1070, 1591
BceAI ACGGC 3 cut(s) 506, 1461, 1653
BciT130I CCWGG 4 cut(s) 426, 729, 865, 1695
BcnI CCSGG 1 cut(s) 1671
BcoDI GTCTC 5 cut(s) 48, 302, 863, 896, 1728
BfaI CTAG 3 cut(s) 374, 1031, 1158
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 2 cut(s) 1643, 1678
BfrI CTTAAG 1 cut(s) 569
BglI GCCNNNNNGGC 1 cut(s) 1648
BisI GCNGC 4 cut(s) 200, 203, 222, 1560
BlsI GCNGC 4 cut(s) 201, 204, 223, 1561
Bme1390I CCNGG 5 cut(s) 426, 729, 865, 1671, 1695
Bme18I GGWCC 3 cut(s) 485, 547, 1408
BmgT120I GGNCC 3 cut(s) 485, 547, 1408
BmiI GGNNCC 7 cut(s) 549, 1101, 1409, 1542, 1641, 1676, 1699
BmrFI CCNGG 5 cut(s) 426, 729, 865, 1671, 1695
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 5 cut(s) 81, 87, 285, 439, 812
BmtI GCTAGC 1 cut(s) 377
BmuI ACTGGG 1 cut(s) 1020
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 1106
Bpu10I CCTNAGC 2 cut(s) 1023, 1194
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 1671
Bsa29I ATCGAT 1 cut(s) 1074
BsaHI GRCGYC 3 cut(s) 1640, 1675, 1705
BsaI GGTCTC 1 cut(s) 48
BsaJI CCNNGG 7 cut(s) 333, 425, 488, 728, 842, 864, 1670
BsaXI ACNNNNNCTCC 8 cut(s) 137, 167, 406, 436, 439, 469, 1681, 1711
Bse1I ACTGG 8 cut(s) 487, 500, 939, 1015, 1089, 1463, 1628, 1652
BseBI CCWGG 4 cut(s) 426, 729, 865, 1695
BseCI ATCGAT 1 cut(s) 1074
BseDI CCNNGG 7 cut(s) 333, 425, 488, 728, 842, 864, 1670
BseGI GGATG 5 cut(s) 239, 430, 760, 766, 1003
BseMII CTCAG 3 cut(s) 642, 1185, 1275
BseNI ACTGG 8 cut(s) 487, 500, 939, 1015, 1089, 1463, 1628, 1652
BseRI GAGGAG 4 cut(s) 378, 381, 914, 1533
BseSI GKGCMC 1 cut(s) 1038
BseXI GCAGC 4 cut(s) 186, 189, 208, 1546
BsgI GTGCAG 1 cut(s) 96
BshFI GGCC 4 cut(s) 180, 493, 1651, 1693
BshNI GGYRCC 2 cut(s) 1639, 1674
BshVI ATCGAT 1 cut(s) 1074
BsiHKAI GWGCWC 2 cut(s) 1038, 1536
BsiSI CCGG 3 cut(s) 181, 1670, 1678
BslFI GGGAC 3 cut(s) 533, 1331, 1421
BsmAI GTCTC 5 cut(s) 48, 302, 863, 896, 1728
BsmFI GGGAC 3 cut(s) 533, 1331, 1421
BsmI GAATGC 2 cut(s) 201, 1144
BsnI GGCC 4 cut(s) 180, 493, 1651, 1693
Bso31I GGTCTC 1 cut(s) 48
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 1038, 1536
Bsp143I GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
BspACI CCGC 8 cut(s) 219, 224, 371, 639, 1057, 1580, 1711, 1725
BspANI GGCC 4 cut(s) 180, 493, 1651, 1693
BspCNI CTCAG 3 cut(s) 641, 1186, 1276
BspDI ATCGAT 1 cut(s) 1074
BspLI GGNNCC 7 cut(s) 549, 1101, 1409, 1542, 1641, 1676, 1699
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 4 cut(s) 1079, 1094, 1107, 1657
BspQI GCTCTTC 1 cut(s) 1569
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 1639, 1674
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 48
BsrI ACTGG 8 cut(s) 487, 500, 939, 1015, 1089, 1463, 1628, 1652
BssECI CCNNGG 7 cut(s) 333, 425, 488, 728, 842, 864, 1670
BssMI GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
BssNI GRCGYC 3 cut(s) 1640, 1675, 1705
BssT1I CCWWGG 2 cut(s) 333, 842
Bst2UI CCWGG 4 cut(s) 426, 729, 865, 1695
Bst4CI ACNGT 5 cut(s) 874, 926, 970, 1429, 1484
Bst6I CTCTTC 3 cut(s) 423, 1367, 1569
BstACI GRCGYC 3 cut(s) 1640, 1675, 1705
BstAFI CTTAAG 1 cut(s) 569
BstAPI GCANNNNNTGC 1 cut(s) 1148
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 4 cut(s) 178, 283, 375, 1057
BstDEI CTNAG 4 cut(s) 628, 1023, 1194, 1284
BstDSI CCRYGG 1 cut(s) 488
BstF5I GGATG 5 cut(s) 239, 430, 760, 766, 1003
BstH2I RGCGCY 2 cut(s) 1643, 1678
BstHHI GCGC 4 cut(s) 1008, 1494, 1642, 1677
BstKTI GATC 6 cut(s) 165, 1074, 1102, 1393, 1438, 1665
BstMAI GTCTC 5 cut(s) 48, 302, 863, 896, 1728
BstMBI GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
BstMWI GCNNNNNNNGC 7 cut(s) 282, 379, 815, 1033, 1148, 1588, 1648
BstNI CCWGG 4 cut(s) 426, 729, 865, 1695
BstNSI RCATGY 2 cut(s) 889, 1346
BstSCI CCNGG 5 cut(s) 424, 727, 863, 1669, 1693
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 1038
BstV1I GCAGC 4 cut(s) 186, 189, 208, 1546
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 2 cut(s) 1099, 1662
BstYI RGATCY 2 cut(s) 1099, 1662
Bsu15I ATCGAT 1 cut(s) 1074
BsuRI GGCC 4 cut(s) 180, 493, 1651, 1693
BsuTUI ATCGAT 1 cut(s) 1074
BtgI CCRYGG 1 cut(s) 488
BtgZI GCGATG 1 cut(s) 262
BtsCI GGATG 5 cut(s) 239, 430, 760, 766, 1003
BtsI GCAGTG 2 cut(s) 788, 1586
BtsIMutI CAGTG 4 cut(s) 788, 1008, 1586, 1737
Cac8I GCNNGC 4 cut(s) 178, 283, 375, 1057
CfoI GCGC 4 cut(s) 1008, 1494, 1642, 1677
Cfr13I GGNCC 3 cut(s) 485, 547, 1408
ClaI ATCGAT 1 cut(s) 1074
CseI GACGC 1 cut(s) 298
CspCI CAANNNNNGTGG 2 cut(s) 1584, 1619
CviAII CATG 6 cut(s) 435, 770, 788, 886, 1343, 1537
DdeI CTNAG 4 cut(s) 628, 1023, 1194, 1284
DinI GGCGCC 2 cut(s) 1641, 1676
DpnI GATC 6 cut(s) 164, 1073, 1101, 1392, 1437, 1664
DpnII GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 3 cut(s) 423, 1367, 1569
EarI CTCTTC 3 cut(s) 423, 1367, 1569
EciI GGCGGA 1 cut(s) 654
Eco130I CCWWGG 2 cut(s) 333, 842
Eco147I AGGCCT 1 cut(s) 1693
Eco31I GGTCTC 1 cut(s) 48
Eco32I GATATC 1 cut(s) 644
Eco47I GGWCC 3 cut(s) 485, 547, 1408
Eco57I CTGAAG 2 cut(s) 938, 1338
EcoO109I RGGNCCY 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 4 cut(s) 424, 727, 863, 1693
EcoRV GATATC 1 cut(s) 644
EcoT14I CCWWGG 2 cut(s) 333, 842
EgeI GGCGCC 2 cut(s) 1641, 1676
EheI GGCGCC 2 cut(s) 1641, 1676
ErhI CCWWGG 2 cut(s) 333, 842
FaeI CATG 6 cut(s) 438, 773, 791, 889, 1346, 1540
FaqI GGGAC 3 cut(s) 533, 1331, 1421
FatI CATG 6 cut(s) 434, 769, 787, 885, 1342, 1536
FauI CCCGC 1 cut(s) 1064
FauNDI CATATG 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 200, 203, 222, 1560
FokI GGATG 5 cut(s) 246, 417, 767, 773, 1010
Fsp4HI GCNGC 4 cut(s) 200, 203, 222, 1560
FspBI CTAG 3 cut(s) 374, 1031, 1158
GlaI GCGC 4 cut(s) 1007, 1493, 1641, 1676
GluI GCNGC 4 cut(s) 200, 203, 222, 1560
GsuI CTGGAG 1 cut(s) 1106
HaeII RGCGCY 2 cut(s) 1643, 1678
HaeIII GGCC 4 cut(s) 180, 493, 1651, 1693
HapII CCGG 3 cut(s) 181, 1670, 1678
HgaI GACGC 1 cut(s) 298
HhaI GCGC 4 cut(s) 1008, 1494, 1642, 1677
Hin1I GRCGYC 3 cut(s) 1640, 1675, 1705
Hin1II CATG 6 cut(s) 438, 773, 791, 889, 1346, 1540
Hin6I GCGC 4 cut(s) 1006, 1492, 1640, 1675
HinP1I GCGC 4 cut(s) 1006, 1492, 1640, 1675
HindIII AAGCTT 1 cut(s) 909
HinfI GANTC 6 cut(s) 263, 322, 329, 668, 686, 1018
HpaII CCGG 3 cut(s) 181, 1670, 1678
HphI GGTGA 4 cut(s) 269, 349, 360, 1700
Hpy166II GTNNAC 3 cut(s) 725, 932, 1036
Hpy188I TCNGA 6 cut(s) 233, 631, 918, 1285, 1433, 1509
Hpy188III TCNNGA 7 cut(s) 47, 326, 606, 1115, 1158, 1316, 1701
Hpy8I GTNNAC 3 cut(s) 725, 932, 1036
HpyAV CCTTC 7 cut(s) 132, 136, 266, 561, 996, 1259, 1313
HpyCH4III ACNGT 5 cut(s) 874, 926, 970, 1429, 1484
HpyCH4IV ACGT 2 cut(s) 449, 1705
HpyCH4V TGCA 8 cut(s) 113, 168, 700, 803, 818, 1036, 1142, 1151
HpyF10VI GCNNNNNNNGC 7 cut(s) 282, 379, 815, 1033, 1148, 1588, 1648
HpyF3I CTNAG 4 cut(s) 628, 1023, 1194, 1284
HpySE526I ACGT 2 cut(s) 449, 1705
Hsp92I GRCGYC 3 cut(s) 1640, 1675, 1705
Hsp92II CATG 6 cut(s) 438, 773, 791, 889, 1346, 1540
HspAI GCGC 4 cut(s) 1006, 1492, 1640, 1675
KasI GGCGCC 2 cut(s) 1639, 1674
Kzo9I GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
LguI GCTCTTC 1 cut(s) 1569
LmnI GCTCC 5 cut(s) 778, 1032, 1087, 1339, 1546
Lsp1109I GCAGC 4 cut(s) 186, 189, 208, 1546
LweI GCATC 5 cut(s) 81, 87, 285, 439, 812
MaeI CTAG 3 cut(s) 374, 1031, 1158
MaeII ACGT 2 cut(s) 449, 1705
MaeIII GTNAC 2 cut(s) 356, 1706
MalI GATC 6 cut(s) 164, 1073, 1101, 1392, 1437, 1664
MboI GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
MflI RGATCY 2 cut(s) 1099, 1662
MhlI GDGCHC 2 cut(s) 1038, 1536
Mly113I GGCGCC 2 cut(s) 1640, 1675
MlyI GAGTC 2 cut(s) 257, 695
MmeI TCCRAC 2 cut(s) 229, 1690
MroXI GAANNNNTTC 2 cut(s) 25, 121
MseI TTAA 3 cut(s) 317, 536, 570
MslI CAYNNNNRTG 1 cut(s) 1521
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 3 cut(s) 181, 1670, 1678
MspR9I CCNGG 5 cut(s) 426, 729, 865, 1671, 1695
Mva1269I GAATGC 2 cut(s) 201, 1144
MvaI CCWGG 4 cut(s) 426, 729, 865, 1695
MwoI GCNNNNNNNGC 7 cut(s) 282, 379, 815, 1033, 1148, 1588, 1648
NarI GGCGCC 2 cut(s) 1640, 1675
NciI CCSGG 1 cut(s) 1671
NdeI CATATG 1 cut(s) 673
NdeII GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
NheI GCTAGC 1 cut(s) 373
NlaIII CATG 6 cut(s) 438, 773, 791, 889, 1346, 1540
NlaIV GGNNCC 7 cut(s) 549, 1101, 1409, 1542, 1641, 1676, 1699
NmuCI GTSAC 1 cut(s) 1706
NspI RCATGY 2 cut(s) 889, 1346
NspV TTCGAA 1 cut(s) 125
PceI AGGCCT 1 cut(s) 1693
PciI ACATGT 1 cut(s) 885
PciSI GCTCTTC 1 cut(s) 1569
PctI GAATGC 2 cut(s) 201, 1144
PdmI GAANNNNTTC 2 cut(s) 25, 121
PfeI GAWTC 4 cut(s) 322, 329, 668, 1018
PkrI GCNGC 4 cut(s) 201, 204, 223, 1561
PleI GAGTC 2 cut(s) 257, 694
PluTI GGCGCC 2 cut(s) 1643, 1678
PpsI GAGTC 2 cut(s) 257, 694
PpuMI RGGWCCY 1 cut(s) 547
PscI ACATGT 1 cut(s) 885
PsiI TTATAA 2 cut(s) 993, 1167
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 4 cut(s) 424, 727, 863, 1693
PspGI CCWGG 4 cut(s) 424, 727, 863, 1693
PspN4I GGNNCC 7 cut(s) 549, 1101, 1409, 1542, 1641, 1676, 1699
PspPI GGNCC 3 cut(s) 485, 547, 1408
PspPPI RGGWCCY 1 cut(s) 547
PsuI RGATCY 2 cut(s) 1099, 1662
RseI CAYNNNNRTG 1 cut(s) 1521
SapI GCTCTTC 1 cut(s) 1569
SaqAI TTAA 3 cut(s) 317, 536, 570
SatI GCNGC 4 cut(s) 200, 203, 222, 1560
Sau3AI GATC 6 cut(s) 162, 1071, 1099, 1390, 1435, 1662
Sau96I GGNCC 3 cut(s) 485, 547, 1408
SchI GAGTC 2 cut(s) 257, 695
ScrFI CCNGG 5 cut(s) 426, 729, 865, 1671, 1695
SduI GDGCHC 2 cut(s) 1038, 1536
SfaNI GCATC 5 cut(s) 81, 87, 285, 439, 812
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 2 cut(s) 1641, 1676
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 3 cut(s) 485, 547, 1408
SmiMI CAYNNNNRTG 1 cut(s) 1521
SmlI CTYRAG 2 cut(s) 134, 569
SmoI CTYRAG 2 cut(s) 134, 569
SseBI AGGCCT 1 cut(s) 1693
SsiI CCGC 8 cut(s) 219, 224, 371, 639, 1057, 1580, 1711, 1725
SspDI GGCGCC 2 cut(s) 1639, 1674
SspMI CTAG 3 cut(s) 374, 1031, 1158
StuI AGGCCT 1 cut(s) 1693
StyD4I CCNGG 5 cut(s) 424, 727, 863, 1669, 1693
StyI CCWWGG 2 cut(s) 333, 842
TaaI ACNGT 5 cut(s) 874, 926, 970, 1429, 1484
TaiI ACGT 2 cut(s) 452, 1708
TaqI TCGA 4 cut(s) 125, 161, 1074, 1403
TfiI GAWTC 4 cut(s) 322, 329, 668, 1018
Tru1I TTAA 3 cut(s) 317, 536, 570
Tru9I TTAA 3 cut(s) 317, 536, 570
TscAI CASTG 4 cut(s) 788, 1015, 1593, 1744
TseFI GTSAC 1 cut(s) 1706
TseI GCWGC 4 cut(s) 199, 202, 221, 1559
Tsp45I GTSAC 1 cut(s) 1706
TspDTI ATGAA 6 cut(s) 543, 606, 660, 968, 1398, 1626
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 4 cut(s) 788, 1015, 1593, 1744
Vha464I CTTAAG 1 cut(s) 569
VneI GTGCAC 1 cut(s) 1034
VpaK11BI GGWCC 3 cut(s) 485, 547, 1408
XapI RAATTY 6 cut(s) 21, 574, 617, 820, 1110, 1250
XbaI TCTAGA 1 cut(s) 1157
XceI RCATGY 2 cut(s) 889, 1346
XmnI GAANNNNTTC 2 cut(s) 25, 121
XspI CTAG 3 cut(s) 374, 1031, 1158
ZraI GACGTC 1 cut(s) 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.