Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009862

Physical Location & Seq

Genomic Coordinates Forward (+)

46166830 .. 46174626

Gene Structure 7797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1341000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGCTCATACAATCTGACGGAATTGTTCTCTTTGTTTGTTTGGGAAAAATTATGAAAAGGTTGAAAATCCTAGGTCAGAGGAAAATCTACTCGACGGCTCCAAGTGTGGCCACTCTTTGTTTAAGTAACATGGACTTGGACCCACCATTTTACAAGGCCTACAAGACCCTTTTCGACAAAACCGTCGACAAAGCCAACAACATCAACAACAACAACAAGGAGGAGGTGTTACAACAGTTTGTTATGGTGGAGGAATGTGAGCTGCCACTGATCAATCTGGGGCGGCTGGAGCTAGGAGAAAAAGAGAGGGAGGAGTGCATGACGGAGATAGCTAGGGCATCGCAAGAGTGGGGTTTCTTTCAGGTAGTGAATCATGGGATTTCAGGAGAGCTTCTTCAGAAGATGAAGAATGAGCAAGAGATGGTGTTCAAGCAATCTTTTGATAAGAAGAGGCAAGAGGACAAGTGCTTGAACTTTTTATCGGGAACTTACCGTTGGGGAACTCCTTCTGCCACATGCCTACGGCAGTTAGCATGGTCTGAAGCTTTTCACATTCCACTCAATGATACTTCTGCTACATCAGCTTCTGGTCTCAATTATACACTCAGCTCAACAATAGAACAATTTGCTACAACAATTTCAAGTTTGGCAGTGAAATTGGCTGAAATTTTGGCTGAGAAATTAGGTCACAAATCAACATTTTTCCAAGAAAATTGTTTTCCCAGCACTTGCTATCTTCGAATGAATCGTTATCCACCGTGCCCCATCCCTTCTGAGGTGTTTGGATTGATGCCACACACTGATAGTGATTTCCTCACAATATTGCATCAAGACCAGGTTGGCGGATTGCAGTTGATCAAAGACGATGAATGGATCGCAGTTAAACCTAATCCAGAGGCTCTAGTCATCAACGTTGGAGATCTATTTCAGGCATGGAGCAACAATGTGTACAAGAGCGTTCAACATCGAGTTGTCACGAACTTACAAGTGGAACGATTCTCAACTGCATATTTCTTATGTCCTTCATATGACACCGTGATACAGAGTTGCAGGGAGCCTTCTGTCTATAGAAAATTCAGCTTTGGGGAGTATAGGCGGCAAGTCCAAGAGGATGTTCAAAAACTGGGTTCGAAAATAGGTCTTCCTAGATTTGTCGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0009685 GO:0009987 GO:0010817 GO:0016101 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0042445 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0045543 GO:0051213 GO:0052635 GO:0055114 GO:0065007 GO:0065008 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

387

Amino Acids

44.03

Weight (kDa)

6.35

Isoelectric Point (pI)

48.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	88 - 188	3.2e-16	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	244 - 341	7.6e-33	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21200 AT4G21200 AT4G21200
fragaria_vesca	FvH4_3g02670
malus_domestica	MD05G1341000.v1.1 MD10G1314500.v1.1
prunus_persica	Prupe.4G026300_v2.0.a1 Prupe.4G026300_v2.0.a1
pyrus_communis	pycom10g26660
rosa_chinensis	RchiOBHm_Chr5g0003991
rosa_laevigata	RLG00000031195
rosa_multiflora	Rmu_sc0000547.1_g000014
rosa_roxburghii	Rroxscaffold_1G00071690 Rroxscaffold_1G00071970
rosa_rugosa	Rorug04G0408800 Rorug04G0408900
rosa_samantha	Rh5AG036600 Rh5BG035700 Rh5CG039200 Rh5DG035100
rosa_wichuraiana	Rw5G003320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	182
AccI	GTMKAC	1 cut(s)	186
AciI	CCGC	3 cut(s)	283, 843, 1096
AclI	AACGTT	1 cut(s)	912
AclWI	GGATC	1 cut(s)	881
AcoI	YGGCCR	1 cut(s)	108
AcsI	RAATTY	2 cut(s)	666, 1073
AcuI	CTGAAG	2 cut(s)	380, 561
AfaI	GTAC	1 cut(s)	950
AfiI	CCNNNNNNNGG	1 cut(s)	775
AgsI	TTSAA	6 cut(s)	64, 430, 472, 642, 962, 1118
AjnI	CCWGG	1 cut(s)	834
AjuI	GAANNNNNNNTTGG	4 cut(s)	478, 510, 1098, 1130
AluBI	AGCT	8 cut(s)	262, 292, 332, 391, 545, 584, 609, 1080
AluI	AGCT	8 cut(s)	262, 292, 332, 391, 545, 584, 609, 1080
Alw26I	GTCTC	1 cut(s)	596
AlwI	GGATC	1 cut(s)	881
AlwNI	CAGNNNCTG	1 cut(s)	587
AoxI	GGCC	2 cut(s)	108, 156
ApeKI	GCWGC	1 cut(s)	262
ApoI	RAATTY	2 cut(s)	666, 1073
Asp700I	GAANNNNTTC	2 cut(s)	505, 546
AspA2I	CCTAGG	1 cut(s)	70
AspS9I	GGNCC	1 cut(s)	139
AsuII	TTCGAA	2 cut(s)	739, 1130
AvaII	GGWCC	1 cut(s)	139
AvrII	CCTAGG	1 cut(s)	70
BaeGI	GKGCMC	1 cut(s)	764
BalI	TGGCCA	1 cut(s)	110
BbsI	GAAGAC	1 cut(s)	1133
BbvI	GCAGC	1 cut(s)	249
BccI	CCATC	2 cut(s)	415, 773
BceAI	ACGGC	2 cut(s)	111, 539
BciT130I	CCWGG	1 cut(s)	836
BclI	TGATCA	2 cut(s)	270, 855
BcoDI	GTCTC	1 cut(s)	596
BfaI	CTAG	5 cut(s)	71, 293, 333, 902, 1146
BfmI	CTRYAG	1 cut(s)	1066
BglII	AGATCT	1 cut(s)	919
BisI	GCNGC	3 cut(s)	263, 284, 1097
BlnI	CCTAGG	1 cut(s)	70
BlsI	GCNGC	3 cut(s)	264, 285, 1098
Bme1390I	CCNGG	1 cut(s)	836
Bme18I	GGWCC	1 cut(s)	139
BmgT120I	GGNCC	1 cut(s)	139
BmiI	GGNNCC	3 cut(s)	99, 141, 1056
BmrFI	CCNGG	1 cut(s)	836
BmrI	ACTGGG	1 cut(s)	1133
BmsI	GCATC	3 cut(s)	347, 780, 835
BmuI	ACTGGG	1 cut(s)	1133
BpiI	GAAGAC	1 cut(s)	1133
BpmI	CTGGAG	1 cut(s)	308
Bpu14I	TTCGAA	2 cut(s)	739, 1130
BsaI	GGTCTC	1 cut(s)	596
BsaJI	CCNNGG	1 cut(s)	70
BsaXI	ACNNNNNCTCC	2 cut(s)	212, 242
Bsc4I	CCNNNNNNNGG	1 cut(s)	775
Bse1I	ACTGG	1 cut(s)	1128
BseBI	CCWGG	1 cut(s)	836
BseDI	CCNNGG	1 cut(s)	70
BseGI	GGATG	2 cut(s)	765, 1117
BseLI	CCNNNNNNNGG	1 cut(s)	775
BseMII	CTCAG	3 cut(s)	619, 666, 765
BseNI	ACTGG	1 cut(s)	1128
BseRI	GAGGAG	2 cut(s)	236, 326
BseSI	GKGCMC	1 cut(s)	764
BseXI	GCAGC	1 cut(s)	249
BseYI	CCCAGC	1 cut(s)	722
BshFI	GGCC	2 cut(s)	110, 158
BslI	CCNNNNNNNGG	1 cut(s)	775
BsmAI	GTCTC	1 cut(s)	596
BsnI	GGCC	2 cut(s)	110, 158
Bso31I	GGTCTC	1 cut(s)	596
Bsp119I	TTCGAA	2 cut(s)	739, 1130
Bsp1286I	GDGCHC	1 cut(s)	764
Bsp1407I	TGTACA	1 cut(s)	948
Bsp143I	GATC	4 cut(s)	270, 855, 873, 919
BspACI	CCGC	3 cut(s)	283, 843, 1096
BspANI	GGCC	2 cut(s)	110, 158
BspCNI	CTCAG	3 cut(s)	618, 667, 766
BspLI	GGNNCC	3 cut(s)	99, 141, 1056
BspPI	GGATC	1 cut(s)	881
BspT104I	TTCGAA	2 cut(s)	739, 1130
BspTNI	GGTCTC	1 cut(s)	596
BsrGI	TGTACA	1 cut(s)	948
BsrI	ACTGG	1 cut(s)	1128
BssECI	CCNNGG	1 cut(s)	70
BssMI	GATC	4 cut(s)	270, 855, 873, 919
BssT1I	CCWWGG	1 cut(s)	70
Bst2UI	CCWGG	1 cut(s)	836
Bst4CI	ACNGT	5 cut(s)	184, 237, 494, 759, 1036
Bst6I	CTCTTC	1 cut(s)	443
BstAUI	TGTACA	1 cut(s)	948
BstBI	TTCGAA	2 cut(s)	739, 1130
BstDEI	CTNAG	3 cut(s)	605, 675, 774
BstF5I	GGATG	2 cut(s)	765, 1117
BstKTI	GATC	4 cut(s)	273, 858, 876, 922
BstMAI	GTCTC	1 cut(s)	596
BstMBI	GATC	4 cut(s)	270, 855, 873, 919
BstMWI	GCNNNNNNNGC	2 cut(s)	289, 581
BstNI	CCWGG	1 cut(s)	836
BstNSI	RCATGY	1 cut(s)	519
BstSCI	CCNGG	1 cut(s)	834
BstSFI	CTRYAG	1 cut(s)	1066
BstSLI	GKGCMC	1 cut(s)	764
BstV1I	GCAGC	1 cut(s)	249
BstV2I	GAAGAC	1 cut(s)	1133
BstX2I	RGATCY	1 cut(s)	919
BstYI	RGATCY	1 cut(s)	919
BsuRI	GGCC	2 cut(s)	110, 158
BtgZI	GCGATG	1 cut(s)	324
BtsCI	GGATG	2 cut(s)	765, 1117
BtsI	GCAGTG	1 cut(s)	657
BtsIMutI	CAGTG	3 cut(s)	266, 657, 798
CaiI	CAGNNNCTG	1 cut(s)	587
Cfr13I	GGNCC	1 cut(s)	139
CsiI	ACCWGGT	1 cut(s)	834
Csp6I	GTAC	1 cut(s)	949
CviAII	CATG	6 cut(s)	130, 319, 374, 516, 534, 933
CviQI	GTAC	1 cut(s)	949
DdeI	CTNAG	3 cut(s)	605, 675, 774
DpnI	GATC	4 cut(s)	272, 857, 875, 921
DpnII	GATC	4 cut(s)	270, 855, 873, 919
DrdI	GACNNNNNNGTC	1 cut(s)	182
DseDI	GACNNNNNNGTC	1 cut(s)	182
EaeI	YGGCCR	1 cut(s)	108
Eam1104I	CTCTTC	1 cut(s)	443
EarI	CTCTTC	1 cut(s)	443
EciI	GGCGGA	1 cut(s)	858
Eco130I	CCWWGG	1 cut(s)	70
Eco147I	AGGCCT	1 cut(s)	158
Eco31I	GGTCTC	1 cut(s)	596
Eco47I	GGWCC	1 cut(s)	139
Eco57I	CTGAAG	2 cut(s)	380, 561
EcoRII	CCWGG	1 cut(s)	834
EcoT14I	CCWWGG	1 cut(s)	70
ErhI	CCWWGG	1 cut(s)	70
FaeI	CATG	6 cut(s)	133, 322, 377, 519, 537, 936
FatI	CATG	6 cut(s)	129, 318, 373, 515, 533, 932
FauNDI	CATATG	1 cut(s)	1027
FbaI	TGATCA	2 cut(s)	270, 855
FblI	GTMKAC	1 cut(s)	186
Fnu4HI	GCNGC	3 cut(s)	263, 284, 1097
FokI	GGATG	2 cut(s)	752, 1124
Fsp4HI	GCNGC	3 cut(s)	263, 284, 1097
FspBI	CTAG	5 cut(s)	71, 293, 333, 902, 1146
GluI	GCNGC	3 cut(s)	263, 284, 1097
GsaI	CCCAGC	1 cut(s)	726
GsuI	CTGGAG	1 cut(s)	308
HaeIII	GGCC	2 cut(s)	110, 158
Hin1II	CATG	6 cut(s)	133, 322, 377, 519, 537, 936
HincII	GTYRAC	1 cut(s)	187
HindII	GTYRAC	1 cut(s)	187
HindIII	AAGCTT	1 cut(s)	543
HinfI	GANTC	3 cut(s)	370, 745, 996
Hpy166II	GTNNAC	2 cut(s)	187, 949
Hpy188I	TCNGA	5 cut(s)	16, 78, 399, 541, 775
Hpy188III	TCNNGA	5 cut(s)	384, 483, 830, 893, 976
Hpy8I	GTNNAC	2 cut(s)	187, 949
Hpy99I	CGWCG	2 cut(s)	97, 188
HpyAV	CCTTC	4 cut(s)	516, 780, 1032, 1068
HpyCH4III	ACNGT	5 cut(s)	184, 237, 494, 759, 1036
HpyCH4IV	ACGT	1 cut(s)	912
HpyCH4V	TGCA	5 cut(s)	318, 826, 850, 1007, 1050
HpyF10VI	GCNNNNNNNGC	2 cut(s)	289, 581
HpyF3I	CTNAG	3 cut(s)	605, 675, 774
HpySE526I	ACGT	1 cut(s)	912
Hsp92II	CATG	6 cut(s)	133, 322, 377, 519, 537, 936
Ksp22I	TGATCA	2 cut(s)	270, 855
Kzo9I	GATC	4 cut(s)	270, 855, 873, 919
LmnI	GCTCC	4 cut(s)	103, 289, 936, 1054
Lsp1109I	GCAGC	1 cut(s)	249
LweI	GCATC	3 cut(s)	347, 780, 835
MabI	ACCWGGT	1 cut(s)	834
MaeI	CTAG	5 cut(s)	71, 293, 333, 902, 1146
MaeII	ACGT	1 cut(s)	912
MaeIII	GTNAC	4 cut(s)	125, 228, 686, 973
MalI	GATC	4 cut(s)	272, 857, 875, 921
MboI	GATC	4 cut(s)	270, 855, 873, 919
MboII	GAAGA	6 cut(s)	386, 412, 418, 460, 728, 1133
MflI	RGATCY	1 cut(s)	919
MhlI	GDGCHC	1 cut(s)	764
MlsI	TGGCCA	1 cut(s)	110
MluNI	TGGCCA	1 cut(s)	110
MmeI	TCCRAC	1 cut(s)	895
Mox20I	TGGCCA	1 cut(s)	110
MroXI	GAANNNNTTC	2 cut(s)	505, 546
MscI	TGGCCA	1 cut(s)	110
MseI	TTAA	2 cut(s)	122, 882
Msp20I	TGGCCA	1 cut(s)	110
MspR9I	CCNGG	1 cut(s)	836
MvaI	CCWGG	1 cut(s)	836
MwoI	GCNNNNNNNGC	2 cut(s)	289, 581
NdeI	CATATG	1 cut(s)	1027
NdeII	GATC	4 cut(s)	270, 855, 873, 919
NlaIII	CATG	6 cut(s)	133, 322, 377, 519, 537, 936
NlaIV	GGNNCC	3 cut(s)	99, 141, 1056
NmuCI	GTSAC	2 cut(s)	686, 973
NspI	RCATGY	1 cut(s)	519
NspV	TTCGAA	2 cut(s)	739, 1130
PceI	AGGCCT	1 cut(s)	158
PcsI	WCGNNNNNNNCGW	2 cut(s)	180, 745
PdmI	GAANNNNTTC	2 cut(s)	505, 546
PfeI	GAWTC	3 cut(s)	370, 745, 996
PkrI	GCNGC	3 cut(s)	264, 285, 1098
Psp1406I	AACGTT	1 cut(s)	912
Psp6I	CCWGG	1 cut(s)	834
PspFI	CCCAGC	1 cut(s)	722
PspGI	CCWGG	1 cut(s)	834
PspN4I	GGNNCC	3 cut(s)	99, 141, 1056
PspPI	GGNCC	1 cut(s)	139
PstNI	CAGNNNCTG	1 cut(s)	587
PsuI	RGATCY	1 cut(s)	919
RsaI	GTAC	1 cut(s)	950
RsaNI	GTAC	1 cut(s)	949
SalI	GTCGAC	1 cut(s)	185
SaqAI	TTAA	2 cut(s)	122, 882
SatI	GCNGC	3 cut(s)	263, 284, 1097
Sau3AI	GATC	4 cut(s)	270, 855, 873, 919
Sau96I	GGNCC	1 cut(s)	139
ScrFI	CCNGG	1 cut(s)	836
SduI	GDGCHC	1 cut(s)	764
SexAI	ACCWGGT	1 cut(s)	834
SfaNI	GCATC	3 cut(s)	347, 780, 835
SfcI	CTRYAG	1 cut(s)	1066
SfuI	TTCGAA	2 cut(s)	739, 1130
SinI	GGWCC	1 cut(s)	139
SseBI	AGGCCT	1 cut(s)	158
SsiI	CCGC	3 cut(s)	283, 843, 1096
SspI	AATATT	1 cut(s)	822
SspMI	CTAG	5 cut(s)	71, 293, 333, 902, 1146
StuI	AGGCCT	1 cut(s)	158
StyD4I	CCNGG	1 cut(s)	834
StyI	CCWWGG	1 cut(s)	70
TaaI	ACNGT	5 cut(s)	184, 237, 494, 759, 1036
TaiI	ACGT	1 cut(s)	915
TaqI	TCGA	6 cut(s)	92, 174, 186, 739, 967, 1130
TatI	WGTACW	1 cut(s)	948
TauI	GCSGC	2 cut(s)	286, 1099
TfiI	GAWTC	3 cut(s)	370, 745, 996
Tru1I	TTAA	2 cut(s)	122, 882
Tru9I	TTAA	2 cut(s)	122, 882
TscAI	CASTG	3 cut(s)	273, 657, 805
TseFI	GTSAC	2 cut(s)	686, 973
TseI	GCWGC	1 cut(s)	262
Tsp45I	GTSAC	2 cut(s)	686, 973
TspDTI	ATGAA	5 cut(s)	68, 419, 758, 882, 1014
TspGWI	ACGGA	2 cut(s)	33, 338
TspRI	CASTG	3 cut(s)	273, 657, 805
VpaK11BI	GGWCC	1 cut(s)	139
XapI	RAATTY	2 cut(s)	666, 1073
XceI	RCATGY	1 cut(s)	519
XmaJI	CCTAGG	1 cut(s)	70
XmiI	GTMKAC	1 cut(s)	186
XmnI	GAANNNNTTC	2 cut(s)	505, 546
XspI	CTAG	5 cut(s)	71, 293, 333, 902, 1146

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.