Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006988

Physical Location & Seq

Genomic Coordinates Forward (+)

709114 .. 713950

Gene Structure 4837 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1006800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2811 bp

ATGAATTTAGGTTTTAGGGTTTTTGAATTGGTGTTTGTGTGTGTTGTTTGCTGGGTTTTGCCATTTGGATTTGGGTCTTCAAATTCAAGTTCAAGACCAGCTGTTGTGAAAATTGGAGCAATTTTCACCTTTGAGTCCACCATTGGAAGAGTTGCCAAGCTTGCCATTCAGGAAGCTGTCAAAGATGTCAACTCCAATTCCAGCATTCTCCATGGCACCAAGCTTGCTGTCAATATGCAGAGCTCCAATTGCAGTGGCTTTCTTGGAATGGTTCAAGCTCTGCAATTCATGGAGACTGATATTGTGGCCATTATAGGCCCACAATCTTCTGTTGTTGCCCATATCATATCCCATGTTTCAAATGAACTCCAAGTTCCCTTATTGTCATTTGCAGCCACAGACCCAACTCTCTCGTCCCTTCAGTTCCCCTTTTTCGTTAGGACAACACACAGTGACTTATATCAAATGACTGCTGTGGCTGAAATGGTTGATCATTACGGTTGGAAGGAGGTTATTGCCATTTATATTGATGATGATTACGGACGCAATGGAGTTTCAACTTTAGATGATAAACTTGCCGAAAGGCGCTGTAGAATTTCATACAAGTTAGGAATTCCCCCTGGACATGGAGCTACCCGGGGTGACCTCATGGATCTTCTTGTTAAGGTCTCGCAATTGGAATCTCGAGTTATAGTTCTTCATGTCAATACTGATTTAGGTCTCAATGTTCTCTCTGTGGCACAATATCTTCAAATGATGGGTGATGGATTTGTGTGGATAGCTACAGATTGGCTATCGTCTCTTTTGGATTCTGCTCTTCCTCTTTCTCCTGAGATCATGGATACGATGCAAGGAGTTCTTGTGCTGCGCCAACACACACCGGATTCTGATAGAAAGAGGGCCTTTTTCTCTAAGTGGAACAAGCTAACTGGTGGTTCCTTGGGACTGCATTCTTATGGGCTTTATGCTTATGATTCTGTTTGGCTGGTTGCTCATGCTATTGATGCATTTTTTAACCAGGGTGGGATTATTTCGTTCTCTAATGATTCTAGGATAAATTCTGTAGACAAAGGTGGTAGTCTTCACCTTGAGGCAATGAGCATATTTGATGATGGTCCGCTCCTATTGAAAAATATATTGCAGAGTAGTTTTCTTGGGTTGACAGGCCCTGTGAAGTTTGATTCAGACAGGTCTCTTGCTCTTCCAGCATATGATATTATTAACGTGCTTGGTACTGGGTTTCGACGAATTGGTTACTGGTGCAACTATTCTGGTTTATCAACTGTCCCCCCTGAGATGCTCTATTCGAAGCCACCTAATCGTTCAAGTGCAAGCCAACAATTATACAGCGTTGTCTGGCCAGGCGAGATATTGTCAAAGCCCCGTGGTTGGGTTTTCCCAAACAATGGGAAACAAATAAGAATTGGTGTGCCTATCCGGGTCAGTTACCGAGAATTTGTATCAAAAGTTCGGGGGACTGATAACATGTTCAAGGGTTTCTGCATAGATGTATTCACAGCTGCTGTAAACTTGTTGCCATATGCTGTTCCATATCGATTTATCCCTTTTGGAGATGGCAAGAACAACCCAAGCTACACAGAGCTTGTGACTTCCATTGCAACGGGTGTCTTTGATGCCGCTATTGGTGACATTGCCATTGTTACAAATCGTACAAAGAATGTGGATTTTTCACAGCCATATGCTGCATCTGGACTTGTTGTAGTGGCTCCGTTTAAGAAATTGAACTCCAGCGCTTGGGCTTTCTTTCGACCATTTACTGCACGTATGTGGGTTGTCACTGCTGCTTCCTTTCTTGTTGTTGGTATAGTTGTGTGGATTTTGGAGCACAGGATAAATGATGAGTTCAGAGGTCCGCCTAAAAAGCAATTAATAACCATCTTGTGGTTTAGCATCTCTACTCTGTTCTTTTCCCATAGAGAGAACACTGTGAGCACCCTTGGTCGTGTAGTGCTAATCATATGGCTCTTTGTGGTTTTGATAATCAACTCAAGTTACACTGCTAGTCTGACATCAATACTCACAGTGCAGCACCTATCTTCTCCTATTAAAGGAATTGAATCCTTGAAAAGTAGCGATGAGCCAATTGGCTATCAAGTTGGTTCCTTTGCTGAACATTATTTGAGTGAGGAACTTGGGATACCCAAGAACAGGCTTATCGCTCTTGGGTCTCCTCAAGCATACGCGCGGGCGCTCCAGCTTGGTCCTAAAAAGGGAGGTGTTGCTGCTGTGGTTGATGAACGCCCTTATGTTGAACTCTTCCTCTCATCGCAGTGCAAATTTAGGGTTGTAGGACCAGAGTTCACCAAAAGCGGATGGGGATTTGCATTTCCGCGGGACTCTCCATTAGCTGTTGACATGTCAACTGCAATTCTACAACTATCAGAGAATGGTGATCTGCAGCGTATCCATGATAAGTGGCTTATGCAAAGCTCTTGCAGTTTAGATACCACTGAACTTGAGTCAGACCGGCTTCATCTTAAAAGTTTTTGGGGCCTCTTTCTCATATGTGGAATTGCTTGCTCCGTTGCTCTCTTCATATATCTTTTGCAAGTTTTGAACAAGTTACGCCGTGCTGAAGTCACACAACGTGTTTCAACTAGTCCTGGCAACTCCCAATCTGGGCGTCTCAAGAGATTTTTATCTATAATTGATGAGCGGAAAGATCAGTCCAGTAGTGGAAGCAAAAGAAAGAAAATCGTTAGATCATTCTCCGACAATGTTACTGATGGCAAGTTGGAATTAAAACAAACAGAAATGACTGCTAGGAGTGACATTAATTCCAACAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007267 GO:0008066 GO:0008150 GO:0008324 GO:0009605 GO:0009606 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009629 GO:0009630 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031347 GO:0031349 GO:0032870 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0042742 GO:0043200 GO:0043207 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046873 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071395 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0080134 GO:0098542 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

937

Amino Acids

103.46

Weight (kDa)

8.74

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	36 - 336	1.6e-11	Periplasmic binding protein
ANF_receptor	PF01094	53 - 410	2.2e-95	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	489 - 576	1e-07	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	494 - 815	2.7e-10	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	594 - 847	9.6e-40	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42540 AT1G42540 AT1G42540 AT1G42540
fragaria_vesca	FvH4_5g25730 FvH4_5g25730 FvH4_5g25730 FvH4_5g25730 FvH4_5g25730 FvH4_5g25730
malus_domestica	MD06G1006800.v1.1
prunus_persica	Prupe.5G008300_v2.0.a1 Prupe.5G008300_v2.0.a1 Prupe.5G008300_v2.0.a1 Prupe.5G008300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0216081
rosa_laevigata	RLG00000002596
rosa_multiflora	Rmu_sc0009767.1_g000004
rosa_roxburghii	Rroxscaffold_3G00243670
rosa_rugosa	Rorug07G0158700
rosa_samantha	Rh7AG295700 Rh7BG288100 Rh7CG316100 Rh7DG298600
rosa_wichuraiana	Rw7G025150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	215
AccB7I	CCANNNNNTGG	3 cut(s)	1406, 1755, 1902
AccBSI	CCGCTC	2 cut(s)	1120, 2677
AccI	GTMKAC	1 cut(s)	1065
AccII	CGCG	3 cut(s)	2204, 2206, 2353
AciI	CCGC	8 cut(s)	1118, 1638, 1874, 2206, 2331, 2351, 2353, 2677
AclWI	GGATC	1 cut(s)	660
AcoI	YGGCCR	2 cut(s)	306, 1358
AcsI	RAATTY	7 cut(s)	4, 82, 594, 612, 1057, 1454, 2297
AcuI	CTGAAG	2 cut(s)	404, 2616
AcyI	GRCGYC	1 cut(s)	2644
AdeI	CACNNNGTG	2 cut(s)	452, 2609
AfaI	GTAC	2 cut(s)	1234, 1672
AfeI	AGCGCT	1 cut(s)	1753
AflIII	ACRYGT	3 cut(s)	1485, 2376, 2608
AhlI	ACTAGT	1 cut(s)	2618
AjnI	CCWGG	4 cut(s)	619, 1017, 1360, 2623
AleI	CACNNNNGTG	1 cut(s)	1786
Alw21I	GWGCWC	3 cut(s)	245, 1848, 1955
Alw26I	GTCTC	7 cut(s)	287, 673, 725, 804, 1197, 2193, 2651
AlwI	GGATC	1 cut(s)	660
AlwNI	CAGNNNCTG	2 cut(s)	1169, 1523
Ama87I	CYCGRG	2 cut(s)	636, 684
Aor51HI	AGCGCT	1 cut(s)	1753
AoxI	GGCC	6 cut(s)	306, 316, 900, 1165, 1358, 2512
ApeKI	GCWGC	8 cut(s)	392, 865, 1520, 1703, 1802, 2047, 2243, 2419
ApoI	RAATTY	7 cut(s)	4, 82, 594, 612, 1057, 1454, 2297
AseI	ATTAAT	2 cut(s)	1889, 2796
AspLEI	GCGC	5 cut(s)	588, 870, 1754, 2206, 2212
AspS9I	GGNCC	8 cut(s)	317, 900, 1115, 1166, 1871, 2222, 2312, 2512
AsuC2I	CCSGG	3 cut(s)	637, 638, 1439
AsuHPI	GGTGA	7 cut(s)	118, 653, 773, 1076, 1658, 2314, 2423
AsuII	TTCGAA	1 cut(s)	1307
AvaI	CYCGRG	2 cut(s)	636, 684
AvaII	GGWCC	4 cut(s)	1115, 1871, 2222, 2312
BalI	TGGCCA	2 cut(s)	308, 1360
BanI	GGYRCC	1 cut(s)	215
BanII	GRGCYC	1 cut(s)	245
BbsI	GAAGAC	2 cut(s)	69, 1073
Bbv12I	GWGCWC	3 cut(s)	245, 1848, 1955
BbvI	GCAGC	8 cut(s)	404, 852, 1507, 1690, 1789, 2059, 2230, 2431
BccI	CCATC	7 cut(s)	751, 758, 1106, 1568, 1904, 2328, 2741
BceAI	ACGGC	1 cut(s)	2574
BciT130I	CCWGG	4 cut(s)	621, 1019, 1362, 2625
BciVI	GTATCC	3 cut(s)	835, 2151, 2435
BclI	TGATCA	1 cut(s)	490
BcnI	CCSGG	3 cut(s)	637, 638, 1439
BcoDI	GTCTC	7 cut(s)	287, 673, 725, 804, 1197, 2193, 2651
BcuI	ACTAGT	1 cut(s)	2618
BfaI	CTAG	4 cut(s)	1050, 2022, 2619, 2784
BfmI	CTRYAG	4 cut(s)	589, 783, 1062, 2417
BfoI	RGCGCY	3 cut(s)	589, 1755, 2213
BfuI	GTATCC	3 cut(s)	835, 2151, 2435
BisI	GCNGC	9 cut(s)	393, 866, 1521, 1638, 1704, 1803, 2048, 2244, 2420
BlsI	GCNGC	9 cut(s)	394, 867, 1522, 1639, 1705, 1804, 2049, 2245, 2421
Bme1390I	CCNGG	7 cut(s)	621, 637, 638, 1019, 1362, 1439, 2625
Bme18I	GGWCC	4 cut(s)	1115, 1871, 2222, 2312
BmeT110I	CYCGRG	2 cut(s)	636, 684
BmgT120I	GGNCC	8 cut(s)	317, 900, 1115, 1166, 1871, 2222, 2312, 2512
BmiI	GGNNCC	5 cut(s)	217, 937, 1728, 2122, 2513
BmrFI	CCNGG	7 cut(s)	621, 637, 638, 1019, 1362, 1439, 2625
BmrI	ACTGGG	1 cut(s)	1245
BmsI	GCATC	6 cut(s)	837, 994, 1287, 1624, 1715, 1920
BmuI	ACTGGG	1 cut(s)	1245
BpiI	GAAGAC	2 cut(s)	69, 1073
BpmI	CTGGAG	2 cut(s)	1732, 2198
Bpu14I	TTCGAA	1 cut(s)	1307
BpuEI	CTTGAG	5 cut(s)	1109, 1993, 2178, 2498, 2633
BpuMI	CCSGG	3 cut(s)	637, 638, 1439
Bsa29I	ATCGAT	1 cut(s)	1555
BsaAI	YACGTR	1 cut(s)	1784
BsaBI	GATNNNNATC	1 cut(s)	2659
BsaHI	GRCGYC	1 cut(s)	2644
BsaI	GGTCTC	4 cut(s)	673, 725, 1197, 2193
BsaJI	CCNNGG	9 cut(s)	211, 619, 636, 637, 939, 1018, 1384, 1957, 2351
BsaWI	WCCGGW	1 cut(s)	880
BsaXI	ACNNNNNCTCC	2 cut(s)	846, 876
Bse118I	RCCGGY	1 cut(s)	2487
Bse1I	ACTGG	4 cut(s)	934, 1240, 1262, 2691
Bse3DI	GCAATG	4 cut(s)	553, 1101, 1614, 1650
Bse8I	GATNNNNATC	1 cut(s)	2659
BseBI	CCWGG	4 cut(s)	621, 1019, 1362, 2625
BseCI	ATCGAT	1 cut(s)	1555
BseDI	CCNNGG	9 cut(s)	211, 619, 636, 637, 939, 1018, 1384, 1957, 2351
BseGI	GGATG	1 cut(s)	2339
BseJI	GATNNNNATC	1 cut(s)	2659
BseMI	GCAATG	4 cut(s)	553, 1101, 1614, 1650
BseMII	CTCAG	2 cut(s)	822, 1284
BseNI	ACTGG	4 cut(s)	934, 1240, 1262, 2691
BseRI	GAGGAG	1 cut(s)	2181
BseXI	GCAGC	8 cut(s)	404, 852, 1507, 1690, 1789, 2059, 2230, 2431
BseYI	CCCAGC	1 cut(s)	51
BsgI	GTGCAG	2 cut(s)	1764, 2066
Bsh1236I	CGCG	3 cut(s)	2204, 2206, 2353
BshFI	GGCC	6 cut(s)	308, 318, 902, 1167, 1360, 2514
BshNI	GGYRCC	1 cut(s)	215
BshVI	ATCGAT	1 cut(s)	1555
BsiHKAI	GWGCWC	3 cut(s)	245, 1848, 1955
BsiHKCI	CYCGRG	2 cut(s)	636, 684
BsiSI	CCGG	4 cut(s)	637, 881, 1438, 2488
BslFI	GGGAC	5 cut(s)	400, 957, 1271, 1489, 2369
BsmAI	GTCTC	7 cut(s)	287, 673, 725, 804, 1197, 2193, 2651
BsmBI	CGTCTC	2 cut(s)	804, 2651
BsmFI	GGGAC	5 cut(s)	400, 957, 1271, 1489, 2369
BsmI	GAATGC	2 cut(s)	204, 949
BsnI	GGCC	6 cut(s)	308, 318, 902, 1167, 1360, 2514
Bso31I	GGTCTC	4 cut(s)	673, 725, 1197, 2193
BsoBI	CYCGRG	2 cut(s)	636, 684
Bsp119I	TTCGAA	1 cut(s)	1307
Bsp1286I	GDGCHC	3 cut(s)	245, 1848, 1955
Bsp143I	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
Bsp19I	CCATGG	1 cut(s)	211
BspACI	CCGC	8 cut(s)	1118, 1638, 1874, 2206, 2331, 2351, 2353, 2677
BspANI	GGCC	6 cut(s)	308, 318, 902, 1167, 1360, 2514
BspCNI	CTCAG	2 cut(s)	823, 1285
BspDI	ATCGAT	1 cut(s)	1555
BspFNI	CGCG	3 cut(s)	2204, 2206, 2353
BspLI	GGNNCC	5 cut(s)	217, 937, 1728, 2122, 2513
BspMAI	CTGCAG	1 cut(s)	2421
BspPI	GGATC	1 cut(s)	660
BspQI	GCTCTTC	2 cut(s)	822, 1206
BspT104I	TTCGAA	1 cut(s)	1307
BspT107I	GGYRCC	1 cut(s)	215
BspTNI	GGTCTC	4 cut(s)	673, 725, 1197, 2193
BsrBI	CCGCTC	2 cut(s)	1120, 2677
BsrDI	GCAATG	4 cut(s)	553, 1101, 1614, 1650
BsrFI	RCCGGY	1 cut(s)	2487
BsrI	ACTGG	4 cut(s)	934, 1240, 1262, 2691
BssAI	RCCGGY	1 cut(s)	2487
BssECI	CCNNGG	9 cut(s)	211, 619, 636, 637, 939, 1018, 1384, 1957, 2351
BssMI	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
BssNI	GRCGYC	1 cut(s)	2644
BssT1I	CCWWGG	3 cut(s)	211, 939, 1957
Bst2UI	CCWGG	4 cut(s)	621, 1019, 1362, 2625
Bst4CI	ACNGT	5 cut(s)	452, 500, 1285, 1948, 2044
Bst6I	CTCTTC	5 cut(s)	142, 822, 1206, 2282, 2558
BstACI	GRCGYC	1 cut(s)	2644
BstBAI	YACGTR	1 cut(s)	1784
BstBI	TTCGAA	1 cut(s)	1307
BstC8I	GCNNGC	5 cut(s)	162, 225, 1333, 2208, 2539
BstDEI	CTNAG	3 cut(s)	831, 912, 1293
BstDSI	CCRYGG	3 cut(s)	211, 1384, 2351
BstEII	GGTNACC	1 cut(s)	641
BstENI	CCTNNNNNAGG	1 cut(s)	2067
BstF5I	GGATG	1 cut(s)	2339
BstFNI	CGCG	3 cut(s)	2204, 2206, 2353
BstH2I	RGCGCY	3 cut(s)	589, 1755, 2213
BstHHI	GCGC	5 cut(s)	588, 870, 1754, 2206, 2212
BstKTI	GATC	6 cut(s)	493, 655, 837, 2416, 2686, 2726
BstMAI	GTCTC	7 cut(s)	287, 673, 725, 804, 1197, 2193, 2651
BstMBI	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
BstMWI	GCNNNNNNNGC	5 cut(s)	161, 249, 1004, 1205, 1882
BstNI	CCWGG	4 cut(s)	621, 1019, 1362, 2625
BstNSI	RCATGY	2 cut(s)	1489, 2380
BstPI	GGTNACC	1 cut(s)	641
BstSCI	CCNGG	7 cut(s)	619, 635, 636, 1017, 1360, 1437, 2623
BstSFI	CTRYAG	4 cut(s)	589, 783, 1062, 2417
BstUI	CGCG	3 cut(s)	2204, 2206, 2353
BstV1I	GCAGC	8 cut(s)	404, 852, 1507, 1690, 1789, 2059, 2230, 2431
BstV2I	GAAGAC	2 cut(s)	69, 1073
BstX2I	RGATCY	1 cut(s)	652
BstYI	RGATCY	1 cut(s)	652
Bsu15I	ATCGAT	1 cut(s)	1555
BsuI	GTATCC	3 cut(s)	835, 2151, 2435
BsuRI	GGCC	6 cut(s)	308, 318, 902, 1167, 1360, 2514
BsuTUI	ATCGAT	1 cut(s)	1555
BtgI	CCRYGG	3 cut(s)	211, 1384, 2351
BtgZI	GCGATG	2 cut(s)	2109, 2271
BtsCI	GGATG	1 cut(s)	2339
BtsI	GCAGTG	4 cut(s)	259, 1797, 2016, 2297
BtsIMutI	CAGTG	8 cut(s)	259, 457, 1797, 1944, 2016, 2049, 2297, 2469
Cac8I	GCNNGC	5 cut(s)	162, 225, 1333, 2208, 2539
CaiI	CAGNNNCTG	2 cut(s)	1169, 1523
CfoI	GCGC	5 cut(s)	588, 870, 1754, 2206, 2212
Cfr10I	RCCGGY	1 cut(s)	2487
Cfr13I	GGNCC	8 cut(s)	317, 900, 1115, 1166, 1871, 2222, 2312, 2512
Cfr42I	CCGCGG	1 cut(s)	2354
Cfr9I	CCCGGG	1 cut(s)	636
ClaI	ATCGAT	1 cut(s)	1555
CseI	GACGC	2 cut(s)	552, 2633
Csp6I	GTAC	2 cut(s)	1233, 1671
CspCI	CAANNNNNGTGG	6 cut(s)	235, 270, 1662, 1697, 2458, 2493
CviQI	GTAC	2 cut(s)	1233, 1671
DdeI	CTNAG	3 cut(s)	831, 912, 1293
DpnI	GATC	6 cut(s)	492, 654, 836, 2415, 2685, 2725
DpnII	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
DraIII	CACNNNGTG	2 cut(s)	452, 2609
EaeI	YGGCCR	2 cut(s)	306, 1358
Eam1104I	CTCTTC	5 cut(s)	142, 822, 1206, 2282, 2558
EarI	CTCTTC	5 cut(s)	142, 822, 1206, 2282, 2558
EciI	GGCGGA	1 cut(s)	1863
Ecl136II	GAGCTC	1 cut(s)	243
Eco130I	CCWWGG	3 cut(s)	211, 939, 1957
Eco24I	GRGCYC	1 cut(s)	245
Eco31I	GGTCTC	4 cut(s)	673, 725, 1197, 2193
Eco47I	GGWCC	4 cut(s)	1115, 1871, 2222, 2312
Eco47III	AGCGCT	1 cut(s)	1753
Eco53kI	GAGCTC	1 cut(s)	243
Eco57I	CTGAAG	2 cut(s)	404, 2616
Eco88I	CYCGRG	2 cut(s)	636, 684
Eco91I	GGTNACC	1 cut(s)	641
EcoICRI	GAGCTC	1 cut(s)	243
EcoNI	CCTNNNNNAGG	1 cut(s)	2067
EcoO109I	RGGNCCY	3 cut(s)	900, 1166, 2512
EcoO65I	GGTNACC	1 cut(s)	641
EcoRI	GAATTC	1 cut(s)	612
EcoRII	CCWGG	4 cut(s)	619, 1017, 1360, 2623
EcoT14I	CCWWGG	3 cut(s)	211, 939, 1957
EcoT22I	ATGCAT	1 cut(s)	1009
EcoT38I	GRGCYC	1 cut(s)	245
ErhI	CCWWGG	3 cut(s)	211, 939, 1957
Esp3I	CGTCTC	2 cut(s)	804, 2651
FalI	AAGNNNNNCTT	4 cut(s)	843, 875, 887, 919
FaqI	GGGAC	5 cut(s)	400, 957, 1271, 1489, 2369
FauI	CCCGC	2 cut(s)	2199, 2346
FauNDI	CATATG	5 cut(s)	1210, 1540, 1699, 1979, 2525
FbaI	TGATCA	1 cut(s)	490
FblI	GTMKAC	1 cut(s)	1065
Fnu4HI	GCNGC	9 cut(s)	393, 866, 1521, 1638, 1704, 1803, 2048, 2244, 2420
FokI	GGATG	1 cut(s)	2346
FriOI	GRGCYC	1 cut(s)	245
Fsp4HI	GCNGC	9 cut(s)	393, 866, 1521, 1638, 1704, 1803, 2048, 2244, 2420
FspBI	CTAG	4 cut(s)	1050, 2022, 2619, 2784
GlaI	GCGC	5 cut(s)	587, 869, 1753, 2205, 2211
GluI	GCNGC	9 cut(s)	393, 866, 1521, 1638, 1704, 1803, 2048, 2244, 2420
GsaI	CCCAGC	1 cut(s)	55
GsuI	CTGGAG	2 cut(s)	1732, 2198
HaeII	RGCGCY	3 cut(s)	589, 1755, 2213
HaeIII	GGCC	6 cut(s)	308, 318, 902, 1167, 1360, 2514
HapII	CCGG	4 cut(s)	637, 881, 1438, 2488
HgaI	GACGC	2 cut(s)	552, 2633
HhaI	GCGC	5 cut(s)	588, 870, 1754, 2206, 2212
Hin1I	GRCGYC	1 cut(s)	2644
Hin6I	GCGC	5 cut(s)	586, 868, 1752, 2204, 2210
HinP1I	GCGC	5 cut(s)	586, 868, 1752, 2204, 2210
HincII	GTYRAC	4 cut(s)	190, 1161, 2374, 2382
HindII	GTYRAC	4 cut(s)	190, 1161, 2374, 2382
HindIII	AAGCTT	2 cut(s)	158, 221
HpaII	CCGG	4 cut(s)	637, 881, 1438, 2488
HphI	GGTGA	7 cut(s)	118, 653, 773, 1076, 1658, 2314, 2423
Hpy166II	GTNNAC	8 cut(s)	138, 190, 1066, 1161, 1528, 2322, 2374, 2382
Hpy188I	TCNGA	7 cut(s)	889, 1186, 1868, 2028, 2404, 2485, 2734
Hpy188III	TCNNGA	6 cut(s)	93, 170, 684, 830, 1710, 2650
Hpy8I	GTNNAC	8 cut(s)	138, 190, 1066, 1161, 1528, 2322, 2374, 2382
Hpy99I	CGWCG	1 cut(s)	1248
HpyAV	CCTTC	2 cut(s)	428, 499
HpyCH4III	ACNGT	5 cut(s)	452, 500, 1285, 1948, 2044
HpyCH4IV	ACGT	3 cut(s)	1224, 1783, 2608
HpyF10VI	GCNNNNNNNGC	5 cut(s)	161, 249, 1004, 1205, 1882
HpyF3I	CTNAG	3 cut(s)	831, 912, 1293
HpySE526I	ACGT	3 cut(s)	1224, 1783, 2608
Hsp92I	GRCGYC	1 cut(s)	2644
HspAI	GCGC	5 cut(s)	586, 868, 1752, 2204, 2210
Ksp22I	TGATCA	1 cut(s)	490
KspI	CCGCGG	1 cut(s)	2354
Kzo9I	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
LguI	GCTCTTC	2 cut(s)	822, 1206
LmnI	GCTCC	8 cut(s)	116, 248, 629, 1125, 1732, 1843, 2217, 2546
Lsp1109I	GCAGC	8 cut(s)	404, 852, 1507, 1690, 1789, 2059, 2230, 2431
LweI	GCATC	6 cut(s)	837, 994, 1287, 1624, 1715, 1920
MaeI	CTAG	4 cut(s)	1050, 2022, 2619, 2784
MaeII	ACGT	3 cut(s)	1224, 1783, 2608
MalI	GATC	6 cut(s)	492, 654, 836, 2415, 2685, 2725
MbiI	CCGCTC	2 cut(s)	1120, 2677
MboI	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
MfeI	CAATTG	3 cut(s)	247, 674, 2103
MflI	RGATCY	1 cut(s)	652
MhlI	GDGCHC	3 cut(s)	245, 1848, 1955
MlsI	TGGCCA	2 cut(s)	308, 1360
MluNI	TGGCCA	2 cut(s)	308, 1360
MlyI	GAGTC	3 cut(s)	143, 2351, 2489
MmeI	TCCRAC	3 cut(s)	482, 2736, 2757
Mox20I	TGGCCA	2 cut(s)	308, 1360
Mph1103I	ATGCAT	1 cut(s)	1009
MscI	TGGCCA	2 cut(s)	308, 1360
MseI	TTAA	9 cut(s)	663, 1014, 1221, 1734, 1889, 2067, 2499, 2762, 2796
MslI	CAYNNNNRTG	3 cut(s)	954, 1786, 2290
Msp20I	TGGCCA	2 cut(s)	308, 1360
MspA1I	CMGCKG	3 cut(s)	101, 1520, 2353
MspI	CCGG	4 cut(s)	637, 881, 1438, 2488
MspR9I	CCNGG	7 cut(s)	621, 637, 638, 1019, 1362, 1439, 2625
MunI	CAATTG	3 cut(s)	247, 674, 2103
Mva1269I	GAATGC	2 cut(s)	204, 949
MvaI	CCWGG	4 cut(s)	621, 1019, 1362, 2625
MvnI	CGCG	3 cut(s)	2204, 2206, 2353
MwoI	GCNNNNNNNGC	5 cut(s)	161, 249, 1004, 1205, 1882
NciI	CCSGG	3 cut(s)	637, 638, 1439
NcoI	CCATGG	1 cut(s)	211
NdeI	CATATG	5 cut(s)	1210, 1540, 1699, 1979, 2525
NdeII	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
NlaIV	GGNNCC	5 cut(s)	217, 937, 1728, 2122, 2513
NmuCI	GTSAC	7 cut(s)	452, 641, 1606, 1646, 1795, 2599, 2789
NsiI	ATGCAT	1 cut(s)	1009
NspI	RCATGY	2 cut(s)	1489, 2380
NspV	TTCGAA	1 cut(s)	1307
OliI	CACNNNNGTG	1 cut(s)	1786
PaeR7I	CTCGAG	1 cut(s)	684
PciI	ACATGT	2 cut(s)	1485, 2376
PciSI	GCTCTTC	2 cut(s)	822, 1206
PctI	GAATGC	2 cut(s)	204, 949
PfeI	GAWTC	7 cut(s)	680, 809, 884, 974, 1046, 1181, 2078
PflMI	CCANNNNNTGG	3 cut(s)	1406, 1755, 1902
PkrI	GCNGC	9 cut(s)	394, 867, 1522, 1639, 1705, 1804, 2049, 2245, 2421
PleI	GAGTC	3 cut(s)	142, 2351, 2488
PpsI	GAGTC	3 cut(s)	142, 2351, 2488
Ppu21I	YACGTR	1 cut(s)	1784
PscI	ACATGT	2 cut(s)	1485, 2376
PshBI	ATTAAT	2 cut(s)	1889, 2796
Psp124BI	GAGCTC	1 cut(s)	245
Psp6I	CCWGG	4 cut(s)	619, 1017, 1360, 2623
PspEI	GGTNACC	1 cut(s)	641
PspFI	CCCAGC	1 cut(s)	51
PspGI	CCWGG	4 cut(s)	619, 1017, 1360, 2623
PspN4I	GGNNCC	5 cut(s)	217, 937, 1728, 2122, 2513
PspPI	GGNCC	8 cut(s)	317, 900, 1115, 1166, 1871, 2222, 2312, 2512
PsrI	GAACNNNNNNTAC	4 cut(s)	1452, 1484, 2142, 2174
PstI	CTGCAG	1 cut(s)	2421
PstNI	CAGNNNCTG	2 cut(s)	1169, 1523
PsuI	RGATCY	1 cut(s)	652
PvuII	CAGCTG	2 cut(s)	101, 1520
RsaI	GTAC	2 cut(s)	1234, 1672
RsaNI	GTAC	2 cut(s)	1233, 1671
RseI	CAYNNNNRTG	3 cut(s)	954, 1786, 2290
SacI	GAGCTC	1 cut(s)	245
SacII	CCGCGG	1 cut(s)	2354
SapI	GCTCTTC	2 cut(s)	822, 1206
SaqAI	TTAA	9 cut(s)	663, 1014, 1221, 1734, 1889, 2067, 2499, 2762, 2796
SatI	GCNGC	9 cut(s)	393, 866, 1521, 1638, 1704, 1803, 2048, 2244, 2420
Sau3AI	GATC	6 cut(s)	490, 652, 834, 2413, 2683, 2723
Sau96I	GGNCC	8 cut(s)	317, 900, 1115, 1166, 1871, 2222, 2312, 2512
SchI	GAGTC	3 cut(s)	143, 2351, 2489
ScrFI	CCNGG	7 cut(s)	621, 637, 638, 1019, 1362, 1439, 2625
SduI	GDGCHC	3 cut(s)	245, 1848, 1955
SfaNI	GCATC	6 cut(s)	837, 994, 1287, 1624, 1715, 1920
SfcI	CTRYAG	4 cut(s)	589, 783, 1062, 2417
Sfr274I	CTCGAG	1 cut(s)	684
Sfr303I	CCGCGG	1 cut(s)	2354
SfuI	TTCGAA	1 cut(s)	1307
SgrBI	CCGCGG	1 cut(s)	2354
SinI	GGWCC	4 cut(s)	1115, 1871, 2222, 2312
SlaI	CTCGAG	1 cut(s)	684
SmaI	CCCGGG	1 cut(s)	638
SmiMI	CAYNNNNRTG	3 cut(s)	954, 1786, 2290
SmlI	CTYRAG	6 cut(s)	684, 1088, 2008, 2193, 2477, 2648
SmoI	CTYRAG	6 cut(s)	684, 1088, 2008, 2193, 2477, 2648
SpeI	ACTAGT	1 cut(s)	2618
SsiI	CCGC	8 cut(s)	1118, 1638, 1874, 2206, 2331, 2351, 2353, 2677
SspMI	CTAG	4 cut(s)	1050, 2022, 2619, 2784
SstI	GAGCTC	1 cut(s)	245
StyD4I	CCNGG	7 cut(s)	619, 635, 636, 1017, 1360, 1437, 2623
StyI	CCWWGG	3 cut(s)	211, 939, 1957
TaaI	ACNGT	5 cut(s)	452, 500, 1285, 1948, 2044
TaiI	ACGT	3 cut(s)	1227, 1786, 2611
TaqI	TCGA	5 cut(s)	685, 1243, 1307, 1555, 1768
TauI	GCSGC	1 cut(s)	1640
TfiI	GAWTC	7 cut(s)	680, 809, 884, 974, 1046, 1181, 2078
Tru1I	TTAA	9 cut(s)	663, 1014, 1221, 1734, 1889, 2067, 2499, 2762, 2796
Tru9I	TTAA	9 cut(s)	663, 1014, 1221, 1734, 1889, 2067, 2499, 2762, 2796
TscAI	CASTG	8 cut(s)	259, 457, 1804, 1951, 2023, 2049, 2297, 2476
TseFI	GTSAC	7 cut(s)	452, 641, 1606, 1646, 1795, 2599, 2789
TseI	GCWGC	8 cut(s)	392, 865, 1520, 1703, 1802, 2047, 2243, 2419
Tsp45I	GTSAC	7 cut(s)	452, 641, 1606, 1646, 1795, 2599, 2789
TspDTI	ATGAA	8 cut(s)	17, 277, 378, 588, 689, 2271, 2483, 2545
TspGWI	ACGGA	3 cut(s)	555, 1719, 2533
TspMI	CCCGGG	1 cut(s)	636
TspRI	CASTG	8 cut(s)	259, 457, 1804, 1951, 2023, 2049, 2297, 2476
Van91I	CCANNNNNTGG	3 cut(s)	1406, 1755, 1902
VpaK11BI	GGWCC	4 cut(s)	1115, 1871, 2222, 2312
VspI	ATTAAT	2 cut(s)	1889, 2796
XagI	CCTNNNNNAGG	1 cut(s)	2067
XapI	RAATTY	7 cut(s)	4, 82, 594, 612, 1057, 1454, 2297
XceI	RCATGY	2 cut(s)	1489, 2380
XhoI	CTCGAG	1 cut(s)	684
XmaI	CCCGGG	1 cut(s)	636
XmiI	GTMKAC	1 cut(s)	1065
XspI	CTAG	4 cut(s)	1050, 2022, 2619, 2784
Zsp2I	ATGCAT	1 cut(s)	1009

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.