Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0006988

Physical Location & Seq

Genomic Coordinates Forward (+)

31447695 .. 31453013

Gene Structure 5319 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G025150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGTTGTCATTTAGGATGGATTTGATCTGGGTTTGTCCCTTGGTGTTTCTTTATCTTGGGGTGTTTTCATTTGGGTCTAGTAATAATCTTTCTTCAAGACCGGCTGTTGTCAACATTGGAGCTCTTTTCACGTTTGACTCTACGATTGGTAAGGTTGCCAAGATTGCTATTGAGGAAGCAGTAAAAGATGTCAACTCCAATTTCAGCATTCTCCATGGGACCAAACTTGTTCCGAAATTTCAGAATACCAATTGCAGTGGGTTTCTTGGAATGGTTGAAGCTTTGCAATTCATGGAGACTGATATCGTTGCCATTATAGGCCCACAATCTTCTGTGGTTGCCCATATTGTATCCCACATTGCAAATGAACTCCAAGTACCGCTATTGTCATTTGCAGCCACAGACCCAACTCTCTCAGCCCTTCAGTTCCCATATTTTATTAGGACAACACAAAGTGATTTGTATCAAATGACTGCAGTTGCTCAAATTGTTGATCATTATGGTTGGAGAGAGGTTATTGCCATTTTTGTAGATGATGATTATGGGCGTAATGGCATATCAGCTTTAGATGATAAACTTGCTGAAAGGCGCTGTAAAATTTCCTACAAGTTGGCAATTCCCCCAGGACCGGCAGCTAATCGGAGTGCCATCATGGATCTGCTTATTAAGGTTGCCTTATTGGAATCTCGCGTTATAGTTCTTCATGTGAATCCTGATTCAGGTTTCATGGTTCTTGCTGTGGCACAGTATCTTAAAATGATGGGTGATGGTTTTGTATGGATAGCTACAGATTGGCTTTCATCTGTTTTAGATTCTGCTTTGCCTCTTCCTTCTGAAATCATGGATACACTGCAAGGAGTTCTTGTTTTGCGGCTACATACACCAGATTCAGATAGAAAGAGGGCCTTCTTTTCTAAGTGGAACAAGCTAACCGGTGGTTCTTTGGGACTGCATACTTATGGGCTTCATGCTTATGATTCTGTTTGGCTGTTAGCTCATGCTATTGATGCGTTTTTCAACCAGGGTGGGGTTATTTCGTTCTCTAATGATTCTAGGATAAAAGCTGTAGAACAAGGTGATAGTCTTCACCTTGATGCAATGAGCATTTTTGATGATGGACCACTCCTGTTGAAGAACATATTGCAAAGTAACCTTGTTGGTTTGACAGGTCCCATTAAGTTTGATTCAGAAAGGGCTCTTGCTCTTCCAGCATATGATGTTATTAATGTGGTTGGAACTGGGTTTCGACGAATTGGTTACTGGTCCAACTATTCTGGGTTATCAACTGTACCTCCTGAGACTCTCTATTCAAAGCCACCTAATCGTTCAAGTGCAAATCAACAGTTATATAGTGTTATCTGGCCAGGGGAGTCATTATTGAAGCCTCGTGGTTGGGTTTTCCCAAACAATGGGATACAATTGAGAATTGGTGTACCTATTCGGGTCAGTTACCGGGAGTTTGTAACGCCAATTCAGGGAACTGATACTTTTAAGGGTTTCTGCATAGATGTATTCAATGCCGCTGTAAACTTGTTGCCATATGCTGTTCCATACAAGTTTATCCCTTTTGGTGATGGTCAAAAAAACCCAAGCTACACAGAGCTTGTGACTTCTATTACAACGGGGGTCTTTGATGCTGCAATTGGCGACATTGCCATTGTTACAAATCGAACGAAGATGGTGGATTTTACACAGCCATATGCTGCATCTGGACTTGTTGTACTGGCCCCCTTTAAAAAATTGAACTCTGGTGCTTGGACCTTCCTTCGACCATTTACTGCACACATGTGGATAGTCACTGCTGCTTCCTTTTTTGTTATTGGTATAGTTGTATGGATTTTGGAGCATAGAATAAATGATGAATTCAGGGGTCCACCAAAGAAGCAAGTTATCACCATCTTCTGGTTTAGCCTCTCAACTCTATTCTTTGCCCATAGAGAGAACACTGTGAGCACCCTTGGTCGAGTAGTGCTACTTATATGGCTGTTTGTGGTTTTGATAATCAACTCTAGTTACACTGCTAGTCTGACATCAATCCTCACAGTGCAGCAGCTATCTTCTCCTATCAAAGGAATTGAAAGCTTGAAAAACAGTGGCGAGCCAATCGGGTATCAAGTGGGTTCTTTTGCTGAGCATTATTTGAGTGAGGAGCTCGGCATATCCAAGTCCAGGCTCATTCCCCTTGCCTCTCCTCTAGCCTATGCTCAGGCACTTCAGCTTGGTCCTAAAAAGGGAGGTGTTGCTGCCGTGGTTGATGAACGCCCGTATGTTGAACTATTCCTCTCAGCACAGTGCAAATTTCGGATTGTAGGTCAAGAGTTCACCAAAGGCGGCTGGGGTTTTGCATTTCCTCGAGATTCTCCTTTAGCTGTTGACATATCAACAGCGATTCTACAATTATCGGAGAATGGTGATCTGCAACGGATCCATGATAAGTGGTTGGTGCAAACCACTTGCAGTTTTGAGAGCACTGAAATTGAATCAGATCAGCTTCAACTCAGAAGCTTTTGGGGCCTCTTTCTCATATGTGGAATTGCTTGCTTCATTGCTCTCCTTGTATATTTTTTGCAAATTGCGAACAAGTTACGCCATGCTGACCCCCCTCAGAGTGTTTCAACTAGTCCTGGTGTTTCACAATCTGGGCGAGTCCGGCGATTTTTATCATTAATTGACAAGAAGGCTGAACAGTCCAGTAGTGGAAGTAAAAAAAGGAAAGTTATTAGATCACTATCCGACAATGATAAGGATGGCAAGTTGGGTTGGCCTCCCAAGAAGAAACAAGAAGAAATGACTGATATGGGTGACATCAAGTCTAGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007267 GO:0008066 GO:0008150 GO:0008324 GO:0009605 GO:0009606 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009629 GO:0009630 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031347 GO:0031349 GO:0032870 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0042742 GO:0043200 GO:0043207 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046873 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071395 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0080134 GO:0098542 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

940

Amino Acids

103.74

Weight (kDa)

8.19

Isoelectric Point (pI)

40.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	46 - 338	3.7e-13	Periplasmic binding protein
ANF_receptor	PF01094	54 - 412	7e-93	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	490 - 576	1.2e-09	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	492 - 814	1.4e-10	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	594 - 847	2.6e-39	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42540 AT1G42540 AT1G42540 AT1G42540
fragaria_vesca	FvH4_5g25730 FvH4_5g25730 FvH4_5g25730 FvH4_5g25730 FvH4_5g25730 FvH4_5g25730
malus_domestica	MD06G1006800.v1.1
prunus_persica	Prupe.5G008300_v2.0.a1 Prupe.5G008300_v2.0.a1 Prupe.5G008300_v2.0.a1 Prupe.5G008300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0216081
rosa_laevigata	RLG00000002596
rosa_multiflora	Rmu_sc0009767.1_g000004
rosa_roxburghii	Rroxscaffold_3G00243670
rosa_rugosa	Rorug07G0158700
rosa_samantha	Rh7AG295700 Rh7BG288100 Rh7CG316100 Rh7DG298600
rosa_wichuraiana	Rw7G025150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	1409, 1902
AccII	CGCG	1 cut(s)	690
AciI	CCGC	4 cut(s)	380, 871, 1521, 2331
AclWI	GGATC	3 cut(s)	663, 2419, 2432
AcoI	YGGCCR	1 cut(s)	1361
AcsI	RAATTY	4 cut(s)	236, 597, 1862, 2297
AcuI	CTGAAG	2 cut(s)	407, 2198
AdeI	CACNNNGTG	1 cut(s)	455
AfaI	GTAC	4 cut(s)	378, 1290, 1434, 1722
AfiI	CCNNNNNNNGG	9 cut(s)	628, 719, 1027, 1409, 1475, 1902, 2069, 2169, 2231
AflIII	ACRYGT	1 cut(s)	1785
AgeI	ACCGGT	1 cut(s)	932
AhdI	GACNNNNNGTC	1 cut(s)	2809
AhlI	ACTAGT	1 cut(s)	2618
AjnI	CCWGG	5 cut(s)	622, 1020, 1363, 2168, 2623
AleI	CACNNNNGTG	1 cut(s)	1786
Alw21I	GWGCWC	4 cut(s)	124, 1955, 2154, 2471
Alw26I	GTCTC	2 cut(s)	290, 1292
AlwI	GGATC	3 cut(s)	663, 2419, 2432
Ama87I	CYCGRG	1 cut(s)	2352
AoxI	GGCC	6 cut(s)	319, 903, 1361, 1725, 2512, 2762
ApeKI	GCWGC	8 cut(s)	395, 632, 1637, 1703, 1802, 2047, 2050, 2243
ApoI	RAATTY	4 cut(s)	236, 597, 1862, 2297
AseI	ATTAAT	2 cut(s)	1224, 2666
AsiGI	ACCGGT	1 cut(s)	932
AspLEI	GCGC	1 cut(s)	591
AsuC2I	CCSGG	1 cut(s)	1454
AsuHPI	GGTGA	8 cut(s)	776, 1079, 1088, 1583, 1885, 2314, 2423, 2813
AvaI	CYCGRG	1 cut(s)	2352
AvaII	GGWCC	8 cut(s)	219, 626, 1118, 1169, 1263, 1758, 1871, 2222
BalI	TGGCCA	1 cut(s)	1363
BamHI	GGATCC	1 cut(s)	2424
BanII	GRGCYC	3 cut(s)	124, 1198, 2154
BauI	CACGAG	1 cut(s)	1386
BbsI	GAAGAC	1 cut(s)	1076
Bbv12I	GWGCWC	4 cut(s)	124, 1955, 2154, 2471
BbvI	GCAGC	8 cut(s)	407, 644, 1624, 1690, 1789, 2059, 2062, 2230
BccI	CCATC	9 cut(s)	10, 656, 754, 761, 1109, 1568, 1672, 1904, 2741
BceAI	ACGGC	1 cut(s)	2231
BciT130I	CCWGG	5 cut(s)	624, 1022, 1365, 2170, 2625
BciVI	GTATCC	3 cut(s)	361, 838, 1407
BclI	TGATCA	1 cut(s)	493
BcnI	CCSGG	1 cut(s)	1454
BcoDI	GTCTC	2 cut(s)	290, 1292
BcuI	ACTAGT	1 cut(s)	2618
BfaI	CTAG	7 cut(s)	78, 1053, 2010, 2022, 2195, 2619, 2814
BfmI	CTRYAG	3 cut(s)	474, 786, 1065
BfoI	RGCGCY	1 cut(s)	592
BfuI	GTATCC	3 cut(s)	361, 838, 1407
BlpI	GCTNAGC	1 cut(s)	2130
Bme1390I	CCNGG	6 cut(s)	624, 1022, 1365, 1454, 2170, 2625
Bme18I	GGWCC	8 cut(s)	219, 626, 1118, 1169, 1263, 1758, 1871, 2222
BmeRI	GACNNNNNGTC	1 cut(s)	2809
BmeT110I	CYCGRG	1 cut(s)	2352
BmiI	GGNNCC	6 cut(s)	220, 1171, 1728, 1872, 2426, 2513
BmrFI	CCNGG	6 cut(s)	624, 1022, 1365, 1454, 2170, 2625
BmrI	ACTGGG	1 cut(s)	1248
BmsI	GCATC	4 cut(s)	997, 1084, 1624, 1715
BmuI	ACTGGG	1 cut(s)	1248
BpiI	GAAGAC	1 cut(s)	1076
Bpu10I	CCTNAGC	1 cut(s)	2205
Bpu1102I	GCTNAGC	1 cut(s)	2130
BpuMI	CCSGG	1 cut(s)	1454
BsaBI	GATNNNNATC	3 cut(s)	462, 2659, 2728
BsaJI	CCNNGG	7 cut(s)	39, 214, 622, 1021, 1364, 1957, 2247
BsaWI	WCCGGW	1 cut(s)	932
BsaXI	ACNNNNNCTCC	4 cut(s)	849, 879, 1276, 1306
Bsc4I	CCNNNNNNNGG	9 cut(s)	628, 719, 1027, 1409, 1475, 1902, 2069, 2169, 2231
Bse118I	RCCGGY	3 cut(s)	100, 628, 932
Bse1I	ACTGG	4 cut(s)	1243, 1265, 1728, 2691
Bse3DI	GCAATG	4 cut(s)	357, 1104, 1650, 2544
Bse8I	GATNNNNATC	3 cut(s)	462, 2659, 2728
BseBI	CCWGG	5 cut(s)	624, 1022, 1365, 2170, 2625
BseDI	CCNNGG	7 cut(s)	39, 214, 622, 1021, 1364, 1957, 2247
BseGI	GGATG	2 cut(s)	21, 2752
BseJI	GATNNNNATC	3 cut(s)	462, 2659, 2728
BseLI	CCNNNNNNNGG	9 cut(s)	628, 719, 1027, 1409, 1475, 1902, 2069, 2169, 2231
BseMI	GCAATG	4 cut(s)	357, 1104, 1650, 2544
BseMII	CTCAG	7 cut(s)	429, 1287, 2121, 2219, 2298, 2512, 2618
BseNI	ACTGG	4 cut(s)	1243, 1265, 1728, 2691
BseRI	GAGGAG	2 cut(s)	2162, 2181
BseXI	GCAGC	8 cut(s)	407, 644, 1624, 1690, 1789, 2059, 2062, 2230
BseYI	CCCAGC	1 cut(s)	2334
BsgI	GTGCAG	2 cut(s)	1764, 2066
Bsh1236I	CGCG	1 cut(s)	690
BshFI	GGCC	6 cut(s)	321, 905, 1363, 1727, 2514, 2764
BshTI	ACCGGT	1 cut(s)	932
BsiHKAI	GWGCWC	4 cut(s)	124, 1955, 2154, 2471
BsiHKCI	CYCGRG	1 cut(s)	2352
BsiSI	CCGG	5 cut(s)	101, 629, 933, 1453, 2650
BslFI	GGGAC	4 cut(s)	21, 232, 960, 1155
BslI	CCNNNNNNNGG	9 cut(s)	628, 719, 1027, 1409, 1475, 1902, 2069, 2169, 2231
BsmAI	GTCTC	2 cut(s)	290, 1292
BsmFI	GGGAC	4 cut(s)	21, 232, 960, 1155
BsmI	GAATGC	1 cut(s)	207
BsnI	GGCC	6 cut(s)	321, 905, 1363, 1727, 2514, 2764
BsoBI	CYCGRG	1 cut(s)	2352
Bsp1286I	GDGCHC	5 cut(s)	124, 1198, 1955, 2154, 2471
Bsp143I	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
Bsp1720I	GCTNAGC	1 cut(s)	2130
Bsp19I	CCATGG	1 cut(s)	214
BspACI	CCGC	4 cut(s)	380, 871, 1521, 2331
BspANI	GGCC	6 cut(s)	321, 905, 1363, 1727, 2514, 2764
BspCNI	CTCAG	7 cut(s)	428, 1288, 2122, 2218, 2297, 2511, 2617
BspFNI	CGCG	1 cut(s)	690
BspLI	GGNNCC	6 cut(s)	220, 1171, 1728, 1872, 2426, 2513
BspMAI	CTGCAG	1 cut(s)	478
BspPI	GGATC	3 cut(s)	663, 2419, 2432
BspQI	GCTCTTC	1 cut(s)	1209
BsrDI	GCAATG	4 cut(s)	357, 1104, 1650, 2544
BsrFI	RCCGGY	3 cut(s)	100, 628, 932
BsrI	ACTGG	4 cut(s)	1243, 1265, 1728, 2691
BssAI	RCCGGY	3 cut(s)	100, 628, 932
BssECI	CCNNGG	7 cut(s)	39, 214, 622, 1021, 1364, 1957, 2247
BssMI	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
BssSI	CACGAG	1 cut(s)	1386
BssT1I	CCWWGG	3 cut(s)	39, 214, 1957
Bst2BI	CACGAG	1 cut(s)	1386
Bst2UI	CCWGG	5 cut(s)	624, 1022, 1365, 2170, 2625
Bst4CI	ACNGT	8 cut(s)	747, 1288, 1344, 1948, 2044, 2093, 2292, 2688
Bst6I	CTCTTC	2 cut(s)	831, 1209
BstC8I	GCNNGC	2 cut(s)	2099, 2539
BstDEI	CTNAG	8 cut(s)	415, 915, 1296, 2130, 2205, 2284, 2498, 2604
BstDSI	CCRYGG	2 cut(s)	214, 2247
BstENI	CCTNNNNNAGG	2 cut(s)	717, 2067
BstF5I	GGATG	2 cut(s)	21, 2752
BstFNI	CGCG	1 cut(s)	690
BstH2I	RGCGCY	1 cut(s)	592
BstHHI	GCGC	1 cut(s)	591
BstKTI	GATC	7 cut(s)	27, 496, 658, 2416, 2427, 2488, 2726
BstMAI	GTCTC	2 cut(s)	290, 1292
BstMBI	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
BstMWI	GCNNNNNNNGC	5 cut(s)	164, 1007, 1208, 2511, 2650
BstNI	CCWGG	5 cut(s)	624, 1022, 1365, 2170, 2625
BstNSI	RCATGY	1 cut(s)	1789
BstSCI	CCNGG	6 cut(s)	622, 1020, 1363, 1452, 2168, 2623
BstSFI	CTRYAG	3 cut(s)	474, 786, 1065
BstUI	CGCG	1 cut(s)	690
BstV1I	GCAGC	8 cut(s)	407, 644, 1624, 1690, 1789, 2059, 2062, 2230
BstV2I	GAAGAC	1 cut(s)	1076
BstX2I	RGATCY	2 cut(s)	655, 2424
BstYI	RGATCY	2 cut(s)	655, 2424
BsuI	GTATCC	3 cut(s)	361, 838, 1407
BsuRI	GGCC	6 cut(s)	321, 905, 1363, 1727, 2514, 2764
BtgI	CCRYGG	2 cut(s)	214, 2247
BtsCI	GGATG	2 cut(s)	21, 2752
BtsI	GCAGTG	4 cut(s)	262, 848, 1797, 2016
BtsIMutI	CAGTG	9 cut(s)	262, 848, 1797, 1944, 2016, 2049, 2098, 2297, 2469
Cac8I	GCNNGC	2 cut(s)	2099, 2539
CfoI	GCGC	1 cut(s)	591
Cfr10I	RCCGGY	3 cut(s)	100, 628, 932
Csp6I	GTAC	4 cut(s)	377, 1289, 1433, 1721
CspAI	ACCGGT	1 cut(s)	932
CspCI	CAANNNNNGTGG	6 cut(s)	238, 273, 1006, 1041, 1110, 1145
CviQI	GTAC	4 cut(s)	377, 1289, 1433, 1721
DdeI	CTNAG	8 cut(s)	415, 915, 1296, 2130, 2205, 2284, 2498, 2604
DpnI	GATC	7 cut(s)	26, 495, 657, 2415, 2426, 2487, 2725
DpnII	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
DraI	TTTAAA	1 cut(s)	1735
DraIII	CACNNNGTG	1 cut(s)	455
DriI	GACNNNNNGTC	1 cut(s)	2809
EaeI	YGGCCR	1 cut(s)	1361
Eam1104I	CTCTTC	2 cut(s)	831, 1209
Eam1105I	GACNNNNNGTC	1 cut(s)	2809
EarI	CTCTTC	2 cut(s)	831, 1209
Ecl136II	GAGCTC	2 cut(s)	122, 2152
Eco130I	CCWWGG	3 cut(s)	39, 214, 1957
Eco24I	GRGCYC	3 cut(s)	124, 1198, 2154
Eco32I	GATATC	1 cut(s)	304
Eco47I	GGWCC	8 cut(s)	219, 626, 1118, 1169, 1263, 1758, 1871, 2222
Eco53kI	GAGCTC	2 cut(s)	122, 2152
Eco57I	CTGAAG	2 cut(s)	407, 2198
Eco88I	CYCGRG	1 cut(s)	2352
EcoICRI	GAGCTC	2 cut(s)	122, 2152
EcoNI	CCTNNNNNAGG	2 cut(s)	717, 2067
EcoO109I	RGGNCCY	3 cut(s)	903, 1169, 2512
EcoRI	GAATTC	1 cut(s)	1862
EcoRII	CCWGG	5 cut(s)	622, 1020, 1363, 2168, 2623
EcoRV	GATATC	1 cut(s)	304
EcoT14I	CCWWGG	3 cut(s)	39, 214, 1957
EcoT38I	GRGCYC	3 cut(s)	124, 1198, 2154
ErhI	CCWWGG	3 cut(s)	39, 214, 1957
FalI	AAGNNNNNCTT	6 cut(s)	659, 691, 846, 878, 890, 922
FaqI	GGGAC	4 cut(s)	21, 232, 960, 1155
FauNDI	CATATG	4 cut(s)	1213, 1540, 1699, 2525
FbaI	TGATCA	1 cut(s)	493
FokI	GGATG	2 cut(s)	28, 2759
FriOI	GRGCYC	3 cut(s)	124, 1198, 2154
FspBI	CTAG	7 cut(s)	78, 1053, 2010, 2022, 2195, 2619, 2814
GlaI	GCGC	1 cut(s)	590
GsaI	CCCAGC	1 cut(s)	2338
HaeII	RGCGCY	1 cut(s)	592
HaeIII	GGCC	6 cut(s)	321, 905, 1363, 1727, 2514, 2764
HapII	CCGG	5 cut(s)	101, 629, 933, 1453, 2650
HhaI	GCGC	1 cut(s)	591
Hin6I	GCGC	1 cut(s)	589
HinP1I	GCGC	1 cut(s)	589
HincII	GTYRAC	3 cut(s)	112, 193, 2374
HindII	GTYRAC	3 cut(s)	112, 193, 2374
HindIII	AAGCTT	3 cut(s)	279, 2080, 2503
HpaII	CCGG	5 cut(s)	101, 629, 933, 1453, 2650
HphI	GGTGA	8 cut(s)	776, 1079, 1088, 1583, 1885, 2314, 2423, 2813
Hpy166II	GTNNAC	7 cut(s)	112, 193, 1433, 1528, 1874, 2322, 2374
Hpy188III	TCNNGA	6 cut(s)	96, 713, 1295, 1710, 2315, 2354
Hpy8I	GTNNAC	7 cut(s)	112, 193, 1433, 1528, 1874, 2322, 2374
Hpy99I	CGWCG	1 cut(s)	1251
HpyAV	CCTTC	6 cut(s)	431, 840, 916, 1771, 1775, 2671
HpyCH4III	ACNGT	8 cut(s)	747, 1288, 1344, 1948, 2044, 2093, 2292, 2688
HpyCH4IV	ACGT	1 cut(s)	131
HpyF10VI	GCNNNNNNNGC	5 cut(s)	164, 1007, 1208, 2511, 2650
HpyF3I	CTNAG	8 cut(s)	415, 915, 1296, 2130, 2205, 2284, 2498, 2604
HpySE526I	ACGT	1 cut(s)	131
HspAI	GCGC	1 cut(s)	589
Ksp22I	TGATCA	1 cut(s)	493
Kzo9I	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
LguI	GCTCTTC	1 cut(s)	1209
LmnI	GCTCC	3 cut(s)	119, 1843, 2149
Lsp1109I	GCAGC	8 cut(s)	407, 644, 1624, 1690, 1789, 2059, 2062, 2230
LweI	GCATC	4 cut(s)	997, 1084, 1624, 1715
MaeI	CTAG	7 cut(s)	78, 1053, 2010, 2022, 2195, 2619, 2814
MaeII	ACGT	1 cut(s)	131
MalI	GATC	7 cut(s)	26, 495, 657, 2415, 2426, 2487, 2725
MboI	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
MfeI	CAATTG	3 cut(s)	250, 1418, 1641
MflI	RGATCY	2 cut(s)	655, 2424
MhlI	GDGCHC	5 cut(s)	124, 1198, 1955, 2154, 2471
MlsI	TGGCCA	1 cut(s)	1363
MluNI	TGGCCA	1 cut(s)	1363
MlyI	GAGTC	4 cut(s)	131, 1294, 1379, 2655
MmeI	TCCRAC	4 cut(s)	485, 1213, 1290, 2757
Mox20I	TGGCCA	1 cut(s)	1363
MscI	TGGCCA	1 cut(s)	1363
MseI	TTAA	8 cut(s)	666, 753, 1176, 1224, 1491, 1734, 2666, 2821
MslI	CAYNNNNRTG	4 cut(s)	957, 972, 1092, 1786
Msp20I	TGGCCA	1 cut(s)	1363
MspA1I	CMGCKG	1 cut(s)	1523
MspI	CCGG	5 cut(s)	101, 629, 933, 1453, 2650
MspR9I	CCNGG	6 cut(s)	624, 1022, 1365, 1454, 2170, 2625
MunI	CAATTG	3 cut(s)	250, 1418, 1641
Mva1269I	GAATGC	1 cut(s)	207
MvaI	CCWGG	5 cut(s)	624, 1022, 1365, 2170, 2625
MvnI	CGCG	1 cut(s)	690
MwoI	GCNNNNNNNGC	5 cut(s)	164, 1007, 1208, 2511, 2650
NciI	CCSGG	1 cut(s)	1454
NcoI	CCATGG	1 cut(s)	214
NdeI	CATATG	4 cut(s)	1213, 1540, 1699, 2525
NdeII	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
NlaIV	GGNNCC	6 cut(s)	220, 1171, 1728, 1872, 2426, 2513
NmeAIII	GCCGAG	1 cut(s)	2133
NmuCI	GTSAC	3 cut(s)	1606, 1795, 2801
NspI	RCATGY	1 cut(s)	1789
OliI	CACNNNNGTG	1 cut(s)	1786
PaeR7I	CTCGAG	1 cut(s)	2352
PciI	ACATGT	1 cut(s)	1785
PciSI	GCTCTTC	1 cut(s)	1209
PctI	GAATGC	1 cut(s)	207
PflMI	CCANNNNNTGG	2 cut(s)	1409, 1902
PinAI	ACCGGT	1 cut(s)	932
PleI	GAGTC	4 cut(s)	131, 1294, 1378, 2654
PpsI	GAGTC	4 cut(s)	131, 1294, 1378, 2654
PpuMI	RGGWCCY	1 cut(s)	1169
PscI	ACATGT	1 cut(s)	1785
PshBI	ATTAAT	2 cut(s)	1224, 2666
Psp124BI	GAGCTC	2 cut(s)	124, 2154
Psp5II	RGGWCCY	1 cut(s)	1169
Psp6I	CCWGG	5 cut(s)	622, 1020, 1363, 2168, 2623
PspFI	CCCAGC	1 cut(s)	2334
PspGI	CCWGG	5 cut(s)	622, 1020, 1363, 2168, 2623
PspN4I	GGNNCC	6 cut(s)	220, 1171, 1728, 1872, 2426, 2513
PspPPI	RGGWCCY	1 cut(s)	1169
PsrI	GAACNNNNNNTAC	2 cut(s)	360, 392
PstI	CTGCAG	1 cut(s)	478
PsuI	RGATCY	2 cut(s)	655, 2424
RsaI	GTAC	4 cut(s)	378, 1290, 1434, 1722
RsaNI	GTAC	4 cut(s)	377, 1289, 1433, 1721
RseI	CAYNNNNRTG	4 cut(s)	957, 972, 1092, 1786
SacI	GAGCTC	2 cut(s)	124, 2154
SapI	GCTCTTC	1 cut(s)	1209
SaqAI	TTAA	8 cut(s)	666, 753, 1176, 1224, 1491, 1734, 2666, 2821
Sau3AI	GATC	7 cut(s)	24, 493, 655, 2413, 2424, 2485, 2723
SchI	GAGTC	4 cut(s)	131, 1294, 1379, 2655
ScrFI	CCNGG	6 cut(s)	624, 1022, 1365, 1454, 2170, 2625
SduI	GDGCHC	5 cut(s)	124, 1198, 1955, 2154, 2471
SfaNI	GCATC	4 cut(s)	997, 1084, 1624, 1715
SfcI	CTRYAG	3 cut(s)	474, 786, 1065
Sfr274I	CTCGAG	1 cut(s)	2352
SinI	GGWCC	8 cut(s)	219, 626, 1118, 1169, 1263, 1758, 1871, 2222
SlaI	CTCGAG	1 cut(s)	2352
SmiMI	CAYNNNNRTG	4 cut(s)	957, 972, 1092, 1786
SmlI	CTYRAG	1 cut(s)	2352
SmoI	CTYRAG	1 cut(s)	2352
SpeI	ACTAGT	1 cut(s)	2618
SsiI	CCGC	4 cut(s)	380, 871, 1521, 2331
SspMI	CTAG	7 cut(s)	78, 1053, 2010, 2022, 2195, 2619, 2814
SstI	GAGCTC	2 cut(s)	124, 2154
StyD4I	CCNGG	6 cut(s)	622, 1020, 1363, 1452, 2168, 2623
StyI	CCWWGG	3 cut(s)	39, 214, 1957
TaaI	ACNGT	8 cut(s)	747, 1288, 1344, 1948, 2044, 2093, 2292, 2688
TaiI	ACGT	1 cut(s)	134
TaqI	TCGA	5 cut(s)	1246, 1669, 1768, 1963, 2353
TatI	WGTACW	1 cut(s)	1720
TauI	GCSGC	3 cut(s)	874, 1523, 2334
Tru1I	TTAA	8 cut(s)	666, 753, 1176, 1224, 1491, 1734, 2666, 2821
Tru9I	TTAA	8 cut(s)	666, 753, 1176, 1224, 1491, 1734, 2666, 2821
TscAI	CASTG	9 cut(s)	262, 855, 1804, 1951, 2023, 2049, 2098, 2297, 2476
TseFI	GTSAC	3 cut(s)	1606, 1795, 2801
TseI	GCWGC	8 cut(s)	395, 632, 1637, 1703, 1802, 2047, 2050, 2243
Tsp45I	GTSAC	3 cut(s)	1606, 1795, 2801
TspGWI	ACGGA	1 cut(s)	2437
TspRI	CASTG	9 cut(s)	262, 855, 1804, 1951, 2023, 2049, 2098, 2297, 2476
Van91I	CCANNNNNTGG	2 cut(s)	1409, 1902
VpaK11BI	GGWCC	8 cut(s)	219, 626, 1118, 1169, 1263, 1758, 1871, 2222
VspI	ATTAAT	2 cut(s)	1224, 2666
XagI	CCTNNNNNAGG	2 cut(s)	717, 2067
XapI	RAATTY	4 cut(s)	236, 597, 1862, 2297
XceI	RCATGY	1 cut(s)	1789
XhoI	CTCGAG	1 cut(s)	2352
XspI	CTAG	7 cut(s)	78, 1053, 2010, 2022, 2195, 2619, 2814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.