MD06G1011600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011565
Physical Location & Seq
Forward (+)
1463717 .. 1465165
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1011600.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGAGGAGGGCATGGCGGAGAATGAAATATCACCACCGCACTCAATTCGCCCTCCCCCTTCTTCGCAAATCCACATCTCACCGGAATCCATCTCATCAGCCGTCAAGGTCTTCCTCGACATCCTAATCCGAACCTCACCCCAGCAAATCGGCGCCGCCCTCGCCTCCACCGGAATCATCCCCAGTGCCGAAGTCGTCATACAAGTCCTCAAACTCTCCTACGACTACCCCTTCTCCGCCGTAAAGTTCTTCCAATGGGCCGGGTTGGCCCACAAGCACTCGGCTCAGGCCTGGAACCTGATGGTCGACGTCCTCGGCCGCAACCGGCTTTTCGAGTCCATGTGGGACGCCATCCGCTCGATGCGACAAGAAAAAACCCTCTCGTTGCCCGCCTTCGCCGCCGCCTTCTCCAATTACTGCGCTGCCGGGAAGTTCAACGACGCCGTCATGACCTTTCAAGTCATGGACAAATATGACATCCCACAAGACGTTGTCGCCGCCAATTCCCTCCTCAGCGCAATGTGCCGCGCAGACGGAGGCGTTGAGAGGGCTCTGGACTTCCTCGAGACCATGAAATCGAAGCCCGAATATGGTCCGGATGGCGATTCGTTTGCCATTTTACTTGAAGGACTGGAGAAGGAAGGGGAGGTGGCGAAGGCGAAGACTACGTTTGGGGAGATGGTGGTGAAAGTTGGGTGGGGACTGGAGAACGTGGCAGCTTACGACGCGTTTTTGGCGACGCTGGTGAATGGGGGACAAGCGGAGGAGGCTGTCAAGTTCTTGAGAGTGATGAAGAAGAATGGCTGCTTGCCAGGTTTGAGATTCTTTTCCATCGCTCTTGATACTCTTGTTAAGCGAAAGGATTCAACGAATGCGATTTCGATTTGGGATGTTATGGTTGGTAATGGATTAATGCCTAATTTGTCAATGTACAATGCTATGATTGGATTGTTGTGTGATTGCAATGAGATCGATGACGCGTTTAGGCTCCTTGATCAGATGGTTTTTCATGGTGCTTTTCCGGATTCTTTCACTTACAATGCGATCTTTAAGTGTTTGATTAAGAACAACAAGGTTCGCGAGGCCGGTAAGTTTTTTGTGGAGATGGTGAAGAATGAGTGGCTTCCGACTCATGCTAATTTTGCTGCGGCCATCACCATGTTTTTTGATGGGGACGACCCCGAAATGGGGGTTGAGCTTTGGAACTCTATGCTTGAAAGCAACGTCGAGCCTCTTGACGCCGCTGCCAATGCCTTGCTTTTGGGCCTTTGTAAATTAGATAGGTTGTCGGAGTTGAAGAGGAATGCTGATGTTATGCTTGATAGAAGGATCAACATATATGAATCAACTATGGCGAGCTTGAAGAGTGCCTACTATAAGGATGGGAGAGGTGCAAGGGAGAAATATGATGGCTTGGCGAGGAGGTGGAAGACCTCACTGGTTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

483

Amino Acids

53.47

Weight (kDa)

5.4

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 114 - 225 8.9e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 278 - 420 1e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 302 - 335 7.7e-09 PPR repeat
PPR_2 PF13041 306 - 355 3.5e-13 PPR repeat family
PPR_2 PF13041 341 - 378 1.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 357
AccI GTMKAC 1 cut(s) 306
AccII CGCG 4 cut(s) 528, 728, 980, 1080
AccIII TCCGGA 2 cut(s) 595, 1021
AclWI GGATC 1 cut(s) 1337
AcoI YGGCCR 2 cut(s) 316, 1149
AcyI GRCGYC 5 cut(s) 153, 309, 348, 441, 1239
AfaI GTAC 1 cut(s) 932
AfiI CCNNNNNNNGG 4 cut(s) 590, 1186, 1187, 1188
AflIII ACRYGT 2 cut(s) 726, 978
AgsI TTSAA 7 cut(s) 436, 458, 626, 867, 1217, 1297, 1363
AjnI CCWGG 2 cut(s) 290, 811
AluBI AGCT 3 cut(s) 719, 1198, 1359
AluI AGCT 3 cut(s) 719, 1198, 1359
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 1 cut(s) 1337
Ama87I CYCGRG 1 cut(s) 563
Aor13HI TCCGGA 2 cut(s) 595, 1021
AoxI GGCC 7 cut(s) 258, 267, 288, 316, 1083, 1149, 1264
ApeKI GCWGC 5 cut(s) 422, 716, 804, 1145, 1244
AseI ATTAAT 1 cut(s) 911
Asp700I GAANNNNTTC 1 cut(s) 862
AspLEI GCGC 4 cut(s) 155, 422, 518, 530
AspS9I GGNCC 4 cut(s) 258, 268, 593, 1264
AsuC2I CCSGG 2 cut(s) 262, 427
AsuHPI GGTGA 7 cut(s) 24, 72, 129, 697, 757, 1120, 1147
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 593
BaeI ACNNNNGTAYC 2 cut(s) 834, 867
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 553
BarI GAAGNNNNNNTAC 4 cut(s) 183, 215, 1355, 1387
BbsI GAAGAC 3 cut(s) 103, 668, 1436
BbvCI CCTCAGC 1 cut(s) 512
BbvI GCAGC 5 cut(s) 409, 728, 791, 1132, 1231
BceAI ACGGC 3 cut(s) 86, 224, 428
BcgI CGANNNNNNTGC 6 cut(s) 29, 63, 593, 627, 952, 986
BciT130I CCWGG 2 cut(s) 292, 813
BclI TGATCA 1 cut(s) 994
BcnI CCSGG 2 cut(s) 262, 427
BcoDI GTCTC 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 156
BglI GCCNNNNNGGC 1 cut(s) 266
Bme1390I CCNGG 4 cut(s) 262, 292, 427, 813
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 4 cut(s) 258, 268, 593, 1264
BmiI GGNNCC 3 cut(s) 154, 296, 989
BmrFI CCNGG 4 cut(s) 262, 292, 427, 813
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 351
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 3 cut(s) 103, 668, 1436
BpmI CTGGAG 2 cut(s) 653, 725
Bpu10I CCTNAGC 2 cut(s) 285, 512
BpuEI CTTGAG 1 cut(s) 802
BpuMI CCSGG 2 cut(s) 262, 427
Bsa29I ATCGAT 1 cut(s) 972
BsaHI GRCGYC 5 cut(s) 153, 309, 348, 441, 1239
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 1 cut(s) 313
BsaWI WCCGGW 4 cut(s) 82, 170, 595, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 4 cut(s) 590, 1186, 1187, 1188
Bse118I RCCGGY 2 cut(s) 324, 1085
Bse1I ACTGG 4 cut(s) 183, 636, 708, 1443
Bse3DI GCAATG 2 cut(s) 525, 970
BseAI TCCGGA 2 cut(s) 595, 1021
BseBI CCWGG 2 cut(s) 292, 813
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 7 cut(s) 120, 177, 351, 477, 604, 895, 1387
BseLI CCNNNNNNNGG 4 cut(s) 590, 1186, 1187, 1188
BseMI GCAATG 2 cut(s) 525, 970
BseMII CTCAG 2 cut(s) 299, 526
BseNI ACTGG 4 cut(s) 183, 636, 708, 1443
BseRI GAGGAG 4 cut(s) 20, 500, 779, 1435
BseX3I CGGCCG 1 cut(s) 316
BseXI GCAGC 5 cut(s) 409, 728, 791, 1132, 1231
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 4 cut(s) 528, 728, 980, 1080
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 7 cut(s) 260, 269, 290, 318, 1085, 1151, 1266
BshNI GGYRCC 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 972
BsiEI CGRYCG 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 8 cut(s) 83, 171, 261, 325, 426, 596, 1022, 1086
BslFI GGGAC 4 cut(s) 359, 714, 768, 1187
BslI CCNNNNNNNGG 4 cut(s) 590, 1186, 1187, 1188
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 4 cut(s) 359, 714, 768, 1187
BsmI GAATGC 2 cut(s) 877, 1309
BsnI GGCC 7 cut(s) 260, 269, 290, 318, 1085, 1151, 1266
Bso31I GGTCTC 1 cut(s) 560
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 553
Bsp13I TCCGGA 2 cut(s) 595, 1021
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 4 cut(s) 969, 994, 1044, 1329
Bsp68I TCGCGA 1 cut(s) 1080
BspANI GGCC 7 cut(s) 260, 269, 290, 318, 1085, 1151, 1266
BspCNI CTCAG 2 cut(s) 298, 525
BspDI ATCGAT 1 cut(s) 972
BspEI TCCGGA 2 cut(s) 595, 1021
BspFNI CGCG 4 cut(s) 528, 728, 980, 1080
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 3 cut(s) 154, 296, 989
BspPI GGATC 1 cut(s) 1337
BspT107I GGYRCC 1 cut(s) 152
BspTNI GGTCTC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 357
BsrDI GCAATG 2 cut(s) 525, 970
BsrFI RCCGGY 2 cut(s) 324, 1085
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 4 cut(s) 183, 636, 708, 1443
BssAI RCCGGY 2 cut(s) 324, 1085
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 4 cut(s) 969, 994, 1044, 1329
BssNI GRCGYC 5 cut(s) 153, 309, 348, 441, 1239
Bst2UI CCWGG 2 cut(s) 292, 813
Bst6I CTCTTC 2 cut(s) 1292, 1358
BstACI GRCGYC 5 cut(s) 153, 309, 348, 441, 1239
BstAUI TGTACA 1 cut(s) 930
BstC8I GCNNGC 3 cut(s) 390, 809, 1357
BstDEI CTNAG 2 cut(s) 285, 512
BstF5I GGATG 7 cut(s) 120, 177, 351, 477, 604, 895, 1387
BstFNI CGCG 4 cut(s) 528, 728, 980, 1080
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 4 cut(s) 155, 422, 518, 530
BstKTI GATC 4 cut(s) 972, 997, 1047, 1332
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 4 cut(s) 969, 994, 1044, 1329
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 9 cut(s) 161, 266, 398, 522, 725, 734, 767, 1142, 1250
BstNI CCWGG 2 cut(s) 292, 813
BstSCI CCNGG 4 cut(s) 260, 290, 425, 811
BstUI CGCG 4 cut(s) 528, 728, 980, 1080
BstV1I GCAGC 5 cut(s) 409, 728, 791, 1132, 1231
BstV2I GAAGAC 3 cut(s) 103, 668, 1436
BstZI CGGCCG 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 7 cut(s) 260, 269, 290, 318, 1085, 1151, 1266
BsuTUI ATCGAT 1 cut(s) 972
BtgZI GCGATG 1 cut(s) 817
BtsCI GGATG 7 cut(s) 120, 177, 351, 477, 604, 895, 1387
BtsIMutI CAGTG 2 cut(s) 190, 1436
BtuMI TCGCGA 1 cut(s) 1080
Cac8I GCNNGC 3 cut(s) 390, 809, 1357
CciI TCATGA 1 cut(s) 447
CfoI GCGC 4 cut(s) 155, 422, 518, 530
Cfr10I RCCGGY 2 cut(s) 324, 1085
Cfr13I GGNCC 4 cut(s) 258, 268, 593, 1264
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 6 cut(s) 356, 449, 734, 748, 986, 1247
Csp6I GTAC 1 cut(s) 931
CviAII CATG 8 cut(s) 13, 340, 448, 463, 571, 1010, 1133, 1159
CviQI GTAC 1 cut(s) 931
DdeI CTNAG 2 cut(s) 285, 512
DinI GGCGCC 1 cut(s) 154
DpnI GATC 4 cut(s) 971, 996, 1046, 1331
DpnII GATC 4 cut(s) 969, 994, 1044, 1329
EaeI YGGCCR 2 cut(s) 316, 1149
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 1292, 1358
EarI CTCTTC 2 cut(s) 1292, 1358
EciI GGCGGA 2 cut(s) 32, 226
EclXI CGGCCG 1 cut(s) 316
Eco147I AGGCCT 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 553
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 593
Eco52I CGGCCG 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 290, 811
EcoT38I GRGCYC 1 cut(s) 553
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaeI CATG 8 cut(s) 16, 343, 451, 466, 574, 1013, 1136, 1162
FaqI GGGAC 4 cut(s) 359, 714, 768, 1187
FatI CATG 8 cut(s) 12, 339, 447, 462, 570, 1009, 1132, 1158
FauI CCCGC 1 cut(s) 397
FbaI TGATCA 1 cut(s) 994
FblI GTMKAC 1 cut(s) 306
FokI GGATG 7 cut(s) 107, 164, 338, 464, 611, 902, 1394
FriOI GRGCYC 1 cut(s) 553
GlaI GCGC 4 cut(s) 154, 421, 517, 529
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 2 cut(s) 653, 725
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 7 cut(s) 260, 269, 290, 318, 1085, 1151, 1266
HapII CCGG 8 cut(s) 83, 171, 261, 325, 426, 596, 1022, 1086
HgaI GACGC 6 cut(s) 356, 449, 734, 748, 986, 1247
HhaI GCGC 4 cut(s) 155, 422, 518, 530
Hin1I GRCGYC 5 cut(s) 153, 309, 348, 441, 1239
Hin1II CATG 8 cut(s) 16, 343, 451, 466, 574, 1013, 1136, 1162
Hin6I GCGC 4 cut(s) 153, 420, 516, 528
HinP1I GCGC 4 cut(s) 153, 420, 516, 528
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 9 cut(s) 86, 174, 335, 605, 822, 863, 1025, 1129, 1343
HpaII CCGG 8 cut(s) 83, 171, 261, 325, 426, 596, 1022, 1086
HphI GGTGA 7 cut(s) 24, 72, 129, 697, 757, 1120, 1147
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 4 cut(s) 131, 999, 1128, 1291
Hpy188III TCNNGA 9 cut(s) 448, 554, 565, 596, 781, 839, 1022, 1079, 1235
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 5 cut(s) 311, 443, 728, 742, 1229
HpyAV CCTTC 9 cut(s) 69, 241, 403, 415, 620, 631, 635, 649, 1320
HpyCH4IV ACGT 5 cut(s) 309, 489, 668, 711, 1224
HpyCH4V TGCA 2 cut(s) 963, 1394
HpyF10VI GCNNNNNNNGC 9 cut(s) 161, 266, 398, 522, 725, 734, 767, 1142, 1250
HpyF3I CTNAG 2 cut(s) 285, 512
HpySE526I ACGT 5 cut(s) 309, 489, 668, 711, 1224
Hsp92I GRCGYC 5 cut(s) 153, 309, 348, 441, 1239
Hsp92II CATG 8 cut(s) 16, 343, 451, 466, 574, 1013, 1136, 1162
HspAI GCGC 4 cut(s) 153, 420, 516, 528
KasI GGCGCC 1 cut(s) 152
Kpn2I TCCGGA 2 cut(s) 595, 1021
Ksp22I TGATCA 1 cut(s) 994
Kzo9I GATC 4 cut(s) 969, 994, 1044, 1329
LmnI GCTCC 1 cut(s) 993
Lsp1109I GCAGC 5 cut(s) 409, 728, 791, 1132, 1231
LweI GCATC 1 cut(s) 351
MaeII ACGT 5 cut(s) 309, 489, 668, 711, 1224
MalI GATC 4 cut(s) 971, 996, 1046, 1331
MbiI CCGCTC 1 cut(s) 357
MboI GATC 4 cut(s) 969, 994, 1044, 1329
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 6 cut(s) 45, 412, 502, 919, 1138, 1274
MluI ACGCGT 2 cut(s) 726, 978
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 2 cut(s) 344, 1123
MmeI TCCRAC 2 cut(s) 1151, 1269
MroI TCCGGA 2 cut(s) 595, 1021
MroXI GAANNNNTTC 1 cut(s) 862
MseI TTAA 4 cut(s) 852, 911, 1050, 1062
MslI CAYNNNNRTG 1 cut(s) 1157
MspA1I CMGCKG 1 cut(s) 1244
MspI CCGG 8 cut(s) 83, 171, 261, 325, 426, 596, 1022, 1086
MspR9I CCNGG 4 cut(s) 262, 292, 427, 813
Mva1269I GAATGC 2 cut(s) 877, 1309
MvaI CCWGG 2 cut(s) 292, 813
MvnI CGCG 4 cut(s) 528, 728, 980, 1080
MwoI GCNNNNNNNGC 9 cut(s) 161, 266, 398, 522, 725, 734, 767, 1142, 1250
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 2 cut(s) 262, 427
NdeII GATC 4 cut(s) 969, 994, 1044, 1329
NlaIII CATG 8 cut(s) 16, 343, 451, 466, 574, 1013, 1136, 1162
NlaIV GGNNCC 3 cut(s) 154, 296, 989
NmeAIII GCCGAG 2 cut(s) 260, 294
NruI TCGCGA 1 cut(s) 1080
PaeR7I CTCGAG 1 cut(s) 563
PagI TCATGA 1 cut(s) 447
PceI AGGCCT 1 cut(s) 290
PctI GAATGC 2 cut(s) 877, 1309
PdmI GAANNNNTTC 1 cut(s) 862
PfeI GAWTC 7 cut(s) 86, 174, 605, 822, 863, 1025, 1343
PflFI GACNNNGTC 2 cut(s) 443, 491
PleI GAGTC 2 cut(s) 343, 1123
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 2 cut(s) 343, 1123
PshBI ATTAAT 1 cut(s) 911
Psp6I CCWGG 2 cut(s) 290, 811
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 2 cut(s) 290, 811
PspN4I GGNNCC 3 cut(s) 154, 296, 989
PspPI GGNCC 4 cut(s) 258, 268, 593, 1264
PsyI GACNNNGTC 2 cut(s) 443, 491
RruI TCGCGA 1 cut(s) 1080
RsaI GTAC 1 cut(s) 932
RsaNI GTAC 1 cut(s) 931
RseI CAYNNNNRTG 1 cut(s) 1157
SalI GTCGAC 1 cut(s) 305
SaqAI TTAA 4 cut(s) 852, 911, 1050, 1062
Sau3AI GATC 4 cut(s) 969, 994, 1044, 1329
Sau96I GGNCC 4 cut(s) 258, 268, 593, 1264
SchI GAGTC 2 cut(s) 344, 1123
ScrFI CCNGG 4 cut(s) 262, 292, 427, 813
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 1 cut(s) 351
SfiI GGCCNNNNNGGCC 1 cut(s) 266
SfoI GGCGCC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 593
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 1157
SmlI CTYRAG 2 cut(s) 563, 781
SmoI CTYRAG 2 cut(s) 563, 781
Sse9I AATT 6 cut(s) 45, 412, 502, 919, 1138, 1274
SseBI AGGCCT 1 cut(s) 290
SspDI GGCGCC 1 cut(s) 152
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 4 cut(s) 260, 290, 425, 811
TaiI ACGT 5 cut(s) 312, 492, 671, 714, 1227
TaqI TCGA 9 cut(s) 117, 306, 333, 359, 564, 578, 881, 972, 1227
TasI AATT 6 cut(s) 45, 412, 502, 919, 1138, 1274
TatI WGTACW 1 cut(s) 930
TauI GCSGC 8 cut(s) 158, 321, 401, 404, 500, 528, 1151, 1244
TfiI GAWTC 7 cut(s) 86, 174, 605, 822, 863, 1025, 1343
Tru1I TTAA 4 cut(s) 852, 911, 1050, 1062
Tru9I TTAA 4 cut(s) 852, 911, 1050, 1062
TscAI CASTG 2 cut(s) 190, 1443
TseI GCWGC 5 cut(s) 422, 716, 804, 1145, 1244
TspDTI ATGAA 5 cut(s) 39, 587, 806, 998, 1356
TspGWI ACGGA 1 cut(s) 549
TspRI CASTG 2 cut(s) 190, 1443
Tth111I GACNNNGTC 2 cut(s) 443, 491
VpaK11BI GGWCC 1 cut(s) 593
VspI ATTAAT 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 862
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.