MD06G1025400.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013279
Physical Location & Seq
Forward (+)
3078722 .. 3081093
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1025400.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGTGCTTTGGGAGATCACATTAGGCACCGCCTATTTCTTGGGGCTCCGCCGCACTTACAGACTCGCCCTCAAGATTCAGCGCCGCCTCATCGGCCCTAACCACCCTAAGATCCGTGAATTTGCTCACAGGCGAACACGTGCTGTGTTTGATGTAGTTGTCAAGGTTCACAAGAACGTTCAGCAACGAGACATAGAAGTTGGTAGAAATCTTGGTAACCGGATCCTGCGCTGGCTCGATCGGATGAAACCTGAAGCTGAGATTCGGCGTCCCCTGGACAAACCTCCCACTGGTTCTAAGTCTAGCATGAGTATTACAAAGCAGGTAAACTCCTCCCAGCTCAAAACTCCTGGAAGCACTCAGATAACCAGAAATCAGGACTCTGACAGACAATTGTTTTCAACAATGACAAATATGTGGTCTAAACCGTTGCCTCCAATTACAATGATGATGAGATCACAAAAACCTTCTGGGAATATGACCCACTACCGCCACTTATGCATGAACGCGCCTCAACCATTAAGATCAAACTACAGTACAGGCGGGTTTGGGGGAGTTATACGAAATGATATAATGCAGTGGATGCTGCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

23.01

Weight (kDa)

11.42

Isoelectric Point (pI)

58.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32260 AT2G35480 AT2G35480
fragaria_vesca FvH4_5g22890 FvH4_5g22890
malus_domestica MD06G1025400.v1.1
prunus_persica Prupe.5G029700_v2.0.a1
pyrus_communis pycom06g02050
rosa_chinensis RchiOBHm_Chr7g0211591
rosa_laevigata RLG00000002996
rosa_multiflora Rmu_sc0003760.1_g000037
rosa_roxburghii Rroxscaffold_3G00247710
rosa_rugosa Rorug07G0126100
rosa_samantha Rh7AG258500 Rh7BG252800 Rh7CG275600 Rh7DG266100
rosa_wichuraiana Rw7G022110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 26
AccII CGCG 1 cut(s) 509
AciI CCGC 6 cut(s) 30, 49, 52, 85, 490, 543
AclI AACGTT 1 cut(s) 177
AclWI GGATC 3 cut(s) 106, 217, 230
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 273
AcvI CACGTG 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 268
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 137
AgsI TTSAA 1 cut(s) 402
AjnI CCWGG 2 cut(s) 273, 349
AluBI AGCT 2 cut(s) 257, 340
AluI AGCT 2 cut(s) 257, 340
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 3 cut(s) 106, 217, 230
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 1 cut(s) 119
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AspLEI GCGC 3 cut(s) 84, 231, 511
AspS9I GGNCC 1 cut(s) 95
BamHI GGATCC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 140
BbvI GCAGC 1 cut(s) 573
BciT130I CCWGG 2 cut(s) 275, 351
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 532
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 3 cut(s) 52, 85, 587
BlsI GCNGC 3 cut(s) 53, 86, 588
Bme1390I CCNGG 2 cut(s) 275, 351
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 3 cut(s) 28, 47, 224
BmrFI CCNGG 2 cut(s) 275, 351
BmsI GCATC 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 295
BseBI CCWGG 2 cut(s) 275, 351
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 249, 588
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 249, 374
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 1 cut(s) 322
BseXI GCAGC 1 cut(s) 573
BseYI CCCAGC 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 509
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 1 cut(s) 96
BshNI GGYRCC 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 241
BsiSI CCGG 1 cut(s) 220
BslFI GGGAC 1 cut(s) 255
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 255
BsnI GGCC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 6 cut(s) 15, 111, 222, 238, 455, 524
BspACI CCGC 6 cut(s) 30, 49, 52, 85, 490, 543
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 2 cut(s) 250, 373
BspFNI CGCG 1 cut(s) 509
BspLI GGNNCC 3 cut(s) 28, 47, 224
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 3 cut(s) 106, 217, 230
BspT107I GGYRCC 1 cut(s) 26
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 6 cut(s) 15, 111, 222, 238, 455, 524
BssNI GRCGYC 1 cut(s) 268
Bst2UI CCWGG 2 cut(s) 275, 351
Bst4CI ACNGT 2 cut(s) 429, 536
BstACI GRCGYC 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 583
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 4 cut(s) 108, 258, 297, 360
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 2 cut(s) 249, 588
BstFNI CGCG 1 cut(s) 509
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 3 cut(s) 84, 231, 511
BstKTI GATC 6 cut(s) 18, 114, 225, 241, 458, 527
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 6 cut(s) 15, 111, 222, 238, 455, 524
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 3 cut(s) 93, 498, 583
BstNI CCWGG 2 cut(s) 275, 351
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 2 cut(s) 273, 349
BstSFI CTRYAG 1 cut(s) 532
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 1 cut(s) 573
BstX2I RGATCY 2 cut(s) 111, 222
BstYI RGATCY 2 cut(s) 111, 222
BsuRI GGCC 1 cut(s) 96
BtsCI GGATG 2 cut(s) 249, 588
BtsI GCAGTG 1 cut(s) 584
BtsIMutI CAGTG 2 cut(s) 288, 584
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 233
CfoI GCGC 3 cut(s) 84, 231, 511
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 537
CviAII CATG 2 cut(s) 307, 502
CviJI RGCY 5 cut(s) 46, 96, 235, 257, 340
CviKI_1 RGCY 5 cut(s) 46, 96, 235, 257, 340
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 4 cut(s) 108, 258, 297, 360
DpnI GATC 6 cut(s) 17, 113, 224, 240, 457, 526
DpnII GATC 6 cut(s) 15, 111, 222, 238, 455, 524
EciI GGCGGA 1 cut(s) 38
Eco24I GRGCYC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 273
Eco72I CACGTG 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 273, 349
EcoT22I ATGCAT 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 48
FaeI CATG 2 cut(s) 310, 505
FaiI YATR 8 cut(s) 194, 308, 416, 479, 499, 503, 560, 572
FaqI GGGAC 1 cut(s) 255
FatI CATG 2 cut(s) 306, 501
FauI CCCGC 1 cut(s) 536
Fnu4HI GCNGC 3 cut(s) 52, 85, 587
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 52, 85, 587
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 83, 230, 510
GluI GCNGC 3 cut(s) 52, 85, 587
GsaI CCCAGC 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 1 cut(s) 96
HapII CCGG 1 cut(s) 220
HgaI GACGC 1 cut(s) 257
HhaI GCGC 3 cut(s) 84, 231, 511
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 2 cut(s) 310, 505
Hin6I GCGC 3 cut(s) 82, 229, 509
HinP1I GCGC 3 cut(s) 82, 229, 509
HinfI GANTC 4 cut(s) 63, 76, 262, 380
HpaII CCGG 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 169, 328
Hpy188I TCNGA 3 cut(s) 243, 363, 385
Hpy188III TCNNGA 2 cut(s) 73, 377
Hpy8I GTNNAC 2 cut(s) 169, 328
HpyAV CCTTC 1 cut(s) 477
HpyCH4III ACNGT 2 cut(s) 429, 536
HpyCH4IV ACGT 2 cut(s) 139, 177
HpyCH4V TGCA 3 cut(s) 501, 577, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 498, 583
HpyF3I CTNAG 4 cut(s) 108, 258, 297, 360
HpySE526I ACGT 2 cut(s) 139, 177
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 2 cut(s) 310, 505
HspAI GCGC 3 cut(s) 82, 229, 509
Kzo9I GATC 6 cut(s) 15, 111, 222, 238, 455, 524
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 1 cut(s) 573
LweI GCATC 1 cut(s) 573
MaeI CTAG 1 cut(s) 303
MaeII ACGT 2 cut(s) 139, 177
MaeIII GTNAC 1 cut(s) 215
MalI GATC 6 cut(s) 17, 113, 224, 240, 457, 526
MboI GATC 6 cut(s) 15, 111, 222, 238, 455, 524
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 2 cut(s) 111, 222
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 3 cut(s) 119, 392, 438
MlyI GAGTC 2 cut(s) 57, 374
MnlI CCTC 6 cut(s) 80, 98, 294, 343, 444, 522
Mph1103I ATGCAT 1 cut(s) 503
MseI TTAA 1 cut(s) 521
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 2 cut(s) 275, 351
MunI CAATTG 1 cut(s) 392
MvaI CCWGG 2 cut(s) 275, 351
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 93, 498, 583
NdeII GATC 6 cut(s) 15, 111, 222, 238, 455, 524
NlaIII CATG 2 cut(s) 310, 505
NlaIV GGNNCC 3 cut(s) 28, 47, 224
NsiI ATGCAT 1 cut(s) 503
PfeI GAWTC 2 cut(s) 76, 262
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 3 cut(s) 53, 86, 588
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 2 cut(s) 57, 374
PmaCI CACGTG 1 cut(s) 140
PmlI CACGTG 1 cut(s) 140
PpsI GAGTC 2 cut(s) 57, 374
Ppu21I YACGTR 1 cut(s) 140
Psp1406I AACGTT 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 273, 349
PspCI CACGTG 1 cut(s) 140
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 336
PspGI CCWGG 2 cut(s) 273, 349
PspN4I GGNNCC 3 cut(s) 28, 47, 224
PspPI GGNCC 1 cut(s) 95
PsuI RGATCY 2 cut(s) 111, 222
PvuI CGATCG 1 cut(s) 241
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 3 cut(s) 52, 85, 587
Sau3AI GATC 6 cut(s) 15, 111, 222, 238, 455, 524
Sau96I GGNCC 1 cut(s) 95
SchI GAGTC 2 cut(s) 57, 374
ScrFI CCNGG 2 cut(s) 275, 351
SduI GDGCHC 1 cut(s) 48
SetI ASST 9 cut(s) 142, 168, 180, 253, 259, 286, 327, 342, 469
SfaNI GCATC 1 cut(s) 573
SfcI CTRYAG 1 cut(s) 532
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 3 cut(s) 119, 392, 438
SsiI CCGC 6 cut(s) 30, 49, 52, 85, 490, 543
SspMI CTAG 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 273, 349
TaaI ACNGT 2 cut(s) 429, 536
TaiI ACGT 2 cut(s) 142, 180
TaqI TCGA 1 cut(s) 237
TasI AATT 3 cut(s) 119, 392, 438
TatI WGTACW 1 cut(s) 536
TauI GCSGC 2 cut(s) 54, 87
TfiI GAWTC 2 cut(s) 76, 262
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 2 cut(s) 295, 584
TseI GCWGC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 260, 518
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 2 cut(s) 295, 584
XapI RAATTY 1 cut(s) 119
XspI CTAG 1 cut(s) 303
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.