Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003712

Physical Location & Seq

Genomic Coordinates Reverse (-)

4785377 .. 4791628

Gene Structure 6252 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1037500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGTTTCAAGGTCCTATTCTAGCTTACTTGACCTTACTTCTGGTGACTCCCCAACTTTTGGCCGCGAGAAGAAGAGGTTTCCCCGAGTGGCAACTGTCGCTGGAGTATTGTCTGAGTTTGATGATGACAACAGTAATAGTGTTGGCTCTGATGCTCCGTCATCCATTGCTCAAGAACGGATGATCATAGTGGGAAATCAGCTTCCACTTCGGGCCCATCGGAGAGACGATGGCGAATGGTACTTTAGCTGGGATGAGGATTCACTTCTTTTGCAACTCAAAGATGGCCTGGGAGAAGATGTAGAAGTAGTCTATATTGGTTGCCTTAAAGAAGAAATTGACCCAAGTGAGCAAGATGATGTAGCCCAAACTTTGCTTGATACTTTCAAATGCGTCCCTGCATTTATCCCTCCTGATCTTTTTAGTAAATTTTATCATGGTTTTTGTAAACAACATTTGTGGCCTTTATTTCATTACATGCTTCCTCTCTCACCAGATCTTGGTGGTAGGTTTGATCGGTCTCTATGGCAAGCTTATGTTTCCGTGAACAAGATATTTGCAGATAAAGTAATGGAAGTGATTAGCCCTGATGATGATTTGGTGTGGGTTCATGACTACCATTTGATGGTGTTGCCAACATTTTTGAGGAAGAGGTTTAATAGATTGAGGCTTGGATTCTTTCTCCACAGCCCGTTCCCTTCGTCTGAGATATACAGGACACTTCCTGTAAGAGATGACCTTCTAAGAGCACTTCTGAATTCTGACCTTATTGGCTTCCATACTTTTGATTATGCTAGGCACTTCCTCTCTTGTTGTAGTAGAATGCTTGGCCTTTCATATCAATCTAAGCGAGGATACATCGGGCTCGAGTATTATGGTCGCACTGTAAGCATTAAGATTCTTCCTGTTGGGATACACATACACCAGCTCCAAAATGTGTTGAATCTTCCAGAGACTGAATCTAAAGTTGCCGAGTTGCAAGATCAGTTTAGGGGGAAAACTGTTTTGCTTGGGGTTGATGATATGGACATATTTAAAGGAATCAGCTTGAAACTTTTGGCCATGGAACAATTGCTTAGTCAACATCCTGATAAGAGGGGTAAGGTTGTTTTGGTTCAAGTTGCTAACCCTGCCAGAGGCCGGGGAAAAGATGTCCGGGAGGTCCAGGTAGAAACTGATGCCACAGTGAAGAGGATCAATGAGACATTTGGAAGGGAAGGATACAAACCTGTGGTCTTGATTGATACCCCGCTTCAGTTTTATGAGCGAATTGCTTATTATGTAATTGCGGAGTGTTGTCTTGTTACAGCGGTGAGGGATGGAATGAATCTTATACCCTATGAGTATATTATCTGCCGCCAAGGAAATGAGAAGCTAGATGAGACTTTAGGACTAAACCCAACAACCCCAAAGAAAAGCATGCTAGTGGTATCCGAGTTTATAGGGTGCTCTCCATCACTAAGTGGGGCAATCCGAGTCAACCCGTGGAACATTGATGCTGTTGCCGAAGCTATGGATTCTGCTCTTATGGTGCCAGAGGCAGAAAAACAGTTGCGACACGAGAAACATTATAGGTATGTCATTACACATGATGTTGCATATTGGGCGCGCAGCTTCTTGCAGGATCTTGAAAGGGCATGTAGGGATCATATGAGGAGAAGGTGCTGGGGAATTGGTTTTGGCTTAGGGTTCCGGGTGATTGTTTTGGATCCAAATTTCAGAAAGCTTTCAGTTGAACTTATTGTTTCAGCTTATAAAAGGACCAAGAATCGAGCGATTCTTTTGGATTATGATGGTGCTATGATGCTGCCGGGTTCAATTATTACCACTCCTAATACAGAGGCTGTTGGAATCTTGAACAATTTATGCAATGATCCCAAGAATGTTGTCTTTCTTGTTAGTGGGAAAGACAGAAAGACTTTAACCAAGTGGTTTTCTTCTTGTGAAAAGCTTGGGATTGCTGCAGAGCATGGCTATTATGTGAGGTCAAATCATAATGCCGACTGGGAAACTTGTATCTCTATTCCAGATTTTGATTGGAAACAGATTGCTGAGCCAGTAATGCAGTTATATACCGAAACAACCGATGGTTCTACCATAGAGACCAAAGAAAGTGCTCTTGTTTGGAATTATCAATACGCAGATCCAGATTTTGGCTTTTGTCAGGCTAAGGAGCTTCTAGATCACCTGGAAAGTGTTCTTGCCAATGAGCCAGTGTCTGTCAAGAGTGGTCAGCACATTGTAGAAGTTAAACCTCAGGGTGTCAACAAAGGCCTTGTAGCAGAACGTCTCCTCGTAGCAATGAAACAGAAAGCAATGCTTCCAGATTTTGTTCTGTGTATTGGGGATGACAGGTCCGACGAAGACATGTTTGAGGCGATAATGAGTTCAAGGGACTCTCTCTCTCCGGTGGCTGAAGTGTTTGCTTGCACTGTCGGTCAGAAGCCCAGCAAGGCCAAGTACTACCTGGAAGACACAACTGAGATTTTGAGAATGTTACAGGGGCTGGCCAATGCTTCGGAGCAGTCGGCCAAAAGCGCTGCAGCGCAGCTTTCTCAGCGAGTACTTGCTTTAGAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

861

Amino Acids

97.35

Weight (kDa)

5.71

Isoelectric Point (pI)

45.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	60 - 544	9.5e-189	Glycosyltransferase family 20
Trehalose_PPase	PF02358	594 - 826	9.4e-80	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17770 AT4G17770
fragaria_vesca	FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550
malus_domestica	MD06G1037500.v1.1
prunus_persica	Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1
pyrus_communis	pycom06g03100
rosa_chinensis	RchiOBHm_Chr7g0210321
rosa_laevigata	RLG00000003046
rosa_multiflora	Rmu_sc0004503.1_g000003 Rmu_sc0017069.1_g000008
rosa_roxburghii	Rroxscaffold_3G00248400
rosa_rugosa	Rorug07G0120600 Rorug07G0120700 Rorug07G0120800 Rorug07G0120800 Rorug07G0120900 Rorug07G0121000 Rorug07G0121000 Rorug07G0121000
rosa_samantha	Rh7BG248600
rosa_wichuraiana	Rw7G021630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1755
AccB1I	GGYRCC	1 cut(s)	1531
AccB7I	CCANNNNNTGG	4 cut(s)	59, 500, 625, 2153
AccII	CGCG	2 cut(s)	66, 1609
AciI	CCGC	5 cut(s)	64, 1250, 1289, 1310, 1357
AclWI	GGATC	7 cut(s)	1202, 1632, 1653, 1703, 1716, 1868, 2138
AcoI	YGGCCR	4 cut(s)	61, 1059, 2508, 2529
AcsI	RAATTY	3 cut(s)	428, 757, 1714
AcuI	CTGAAG	2 cut(s)	1238, 2436
AdeI	CACNNNGTG	1 cut(s)	1463
AfaI	GTAC	3 cut(s)	242, 2462, 2565
AfeI	AGCGCT	1 cut(s)	2539
AfiI	CCNNNNNNNGG	6 cut(s)	59, 500, 625, 1136, 1140, 2153
AflIII	ACRYGT	1 cut(s)	2367
AjnI	CCWGG	4 cut(s)	288, 1164, 2187, 2466
Alw21I	GWGCWC	3 cut(s)	751, 1451, 2119
Alw26I	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
AlwI	GGATC	7 cut(s)	1202, 1632, 1653, 1703, 1716, 1868, 2138
AlwNI	CAGNNNCTG	4 cut(s)	956, 1844, 2219, 2506
Ama87I	CYCGRG	2 cut(s)	84, 866
Aor51HI	AGCGCT	1 cut(s)	2539
ApaI	GGGCCC	1 cut(s)	217
ApeKI	GCWGC	6 cut(s)	1611, 1807, 1961, 2540, 2543, 2548
ApoI	RAATTY	3 cut(s)	428, 757, 1714
ArsI	GACNNNNNNTTYG	2 cut(s)	1218, 1250
Asp700I	GAANNNNTTC	2 cut(s)	1726, 2196
AspLEI	GCGC	4 cut(s)	1609, 1611, 2540, 2548
AspS9I	GGNCC	6 cut(s)	12, 213, 214, 1162, 1761, 2355
AsuC2I	CCSGG	4 cut(s)	1142, 1157, 1694, 1812
AsuHPI	GGTGA	5 cut(s)	56, 483, 1324, 1708, 2177
AvaI	CYCGRG	2 cut(s)	84, 866
AvaII	GGWCC	4 cut(s)	12, 1162, 1761, 2355
AxyI	CCTNAGG	1 cut(s)	2256
BaeGI	GKGCMC	1 cut(s)	217
BalI	TGGCCA	2 cut(s)	1061, 2510
BamHI	GGATCC	1 cut(s)	1708
BanI	GGYRCC	1 cut(s)	1531
BanII	GRGCYC	2 cut(s)	217, 867
BauI	CACGAG	1 cut(s)	1559
BbsI	GAAGAC	2 cut(s)	2370, 2478
Bbv12I	GWGCWC	3 cut(s)	751, 1451, 2119
BbvI	GCAGC	6 cut(s)	1623, 1794, 1948, 2527, 2555, 2560
BccI	CCATC	8 cut(s)	224, 225, 278, 619, 1313, 1462, 1787, 2081
BciT130I	CCWGG	4 cut(s)	290, 1166, 2189, 2468
BciVI	GTATCC	4 cut(s)	848, 906, 1214, 1441
BclI	TGATCA	1 cut(s)	183
BcnI	CCSGG	4 cut(s)	1142, 1157, 1694, 1812
BcoDI	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
BfaI	CTAG	5 cut(s)	21, 795, 1376, 1424, 2180
BfmI	CTRYAG	2 cut(s)	1962, 2541
BfoI	RGCGCY	1 cut(s)	2541
BfuI	GTATCC	4 cut(s)	848, 906, 1214, 1441
BglII	AGATCT	1 cut(s)	496
BisI	GCNGC	8 cut(s)	64, 1357, 1612, 1808, 1962, 2541, 2544, 2549
BlpI	GCTNAGC	1 cut(s)	2052
BlsI	GCNGC	8 cut(s)	65, 1358, 1613, 1809, 1963, 2542, 2545, 2550
BmcAI	AGTACT	2 cut(s)	2462, 2565
Bme1390I	CCNGG	8 cut(s)	290, 1142, 1157, 1166, 1694, 1812, 2189, 2468
Bme18I	GGWCC	4 cut(s)	12, 1162, 1761, 2355
BmeT110I	CYCGRG	2 cut(s)	84, 866
BmgT120I	GGNCC	6 cut(s)	12, 213, 214, 1162, 1761, 2355
BmiI	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
BmrFI	CCNGG	8 cut(s)	290, 1142, 1157, 1166, 1694, 1812, 2189, 2468
BmrI	ACTGGG	1 cut(s)	2014
BmsI	GCATC	4 cut(s)	142, 1168, 1486, 1794
BmuI	ACTGGG	1 cut(s)	2014
BpiI	GAAGAC	2 cut(s)	2370, 2478
BpmI	CTGGAG	1 cut(s)	123
Bpu10I	CCTNAGC	2 cut(s)	1684, 2169
Bpu1102I	GCTNAGC	1 cut(s)	2052
BpuEI	CTTGAG	1 cut(s)	156
BpuMI	CCSGG	4 cut(s)	1142, 1157, 1694, 1812
BsaI	GGTCTC	2 cut(s)	525, 2096
BsaJI	CCNNGG	5 cut(s)	289, 1062, 1141, 1360, 1484
BsaWI	WCCGGW	1 cut(s)	2407
BsaXI	ACNNNNNCTCC	4 cut(s)	285, 315, 912, 942
Bsc4I	CCNNNNNNNGG	6 cut(s)	59, 500, 625, 1136, 1140, 2153
Bse1I	ACTGG	3 cut(s)	2009, 2057, 2213
Bse21I	CCTNAGG	1 cut(s)	2256
Bse3DI	GCAATG	4 cut(s)	165, 1876, 2307, 2322
BseBI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
BseDI	CCNNGG	5 cut(s)	289, 1062, 1141, 1360, 1484
BseGI	GGATG	6 cut(s)	161, 186, 259, 1084, 1324, 2353
BseLI	CCNNNNNNNGG	6 cut(s)	59, 500, 625, 1136, 1140, 2153
BseMI	GCAATG	4 cut(s)	165, 1876, 2307, 2322
BseMII	CTCAG	6 cut(s)	105, 696, 2043, 2270, 2472, 2570
BseNI	ACTGG	3 cut(s)	2009, 2057, 2213
BsePI	GCGCGC	1 cut(s)	1607
BseRI	GAGGAG	2 cut(s)	1669, 2282
BseSI	GKGCMC	1 cut(s)	217
BseXI	GCAGC	6 cut(s)	1623, 1794, 1948, 2527, 2555, 2560
BseYI	CCCAGC	3 cut(s)	249, 1665, 2447
Bsh1236I	CGCG	2 cut(s)	66, 1609
BshNI	GGYRCC	1 cut(s)	1531
BsiHKAI	GWGCWC	3 cut(s)	751, 1451, 2119
BsiHKCI	CYCGRG	2 cut(s)	84, 866
BsiSI	CCGG	5 cut(s)	1141, 1156, 1693, 1811, 2408
BslFI	GGGAC	2 cut(s)	380, 2408
BslI	CCNNNNNNNGG	6 cut(s)	59, 500, 625, 1136, 1140, 2153
BsmAI	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
BsmBI	CGTCTC	2 cut(s)	219, 2294
BsmFI	GGGAC	2 cut(s)	380, 2408
BsmI	GAATGC	1 cut(s)	828
Bso31I	GGTCTC	2 cut(s)	525, 2096
BsoBI	CYCGRG	2 cut(s)	84, 866
Bsp120I	GGGCCC	1 cut(s)	213
Bsp1286I	GDGCHC	5 cut(s)	217, 751, 867, 1451, 2119
Bsp1720I	GCTNAGC	1 cut(s)	2052
Bsp19I	CCATGG	1 cut(s)	1062
BspACI	CCGC	5 cut(s)	64, 1250, 1289, 1310, 1357
BspCNI	CTCAG	6 cut(s)	106, 697, 2044, 2269, 2473, 2569
BspFNI	CGCG	2 cut(s)	66, 1609
BspHI	TCATGA	1 cut(s)	610
BspLI	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
BspMAI	CTGCAG	2 cut(s)	1966, 2545
BspPI	GGATC	7 cut(s)	1202, 1632, 1653, 1703, 1716, 1868, 2138
BspT107I	GGYRCC	1 cut(s)	1531
BspTNI	GGTCTC	2 cut(s)	525, 2096
BsrDI	GCAATG	4 cut(s)	165, 1876, 2307, 2322
BsrI	ACTGG	3 cut(s)	2009, 2057, 2213
BssECI	CCNNGG	5 cut(s)	289, 1062, 1141, 1360, 1484
BssHII	GCGCGC	1 cut(s)	1607
BssSI	CACGAG	1 cut(s)	1559
BssT1I	CCWWGG	2 cut(s)	1062, 1360
Bst2BI	CACGAG	1 cut(s)	1559
Bst2UI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
Bst4CI	ACNGT	7 cut(s)	97, 134, 886, 1003, 1186, 1551, 2434
Bst6I	CTCTTC	3 cut(s)	68, 644, 1184
BstC8I	GCNNGC	5 cut(s)	531, 1421, 1609, 2428, 2508
BstDSI	CCRYGG	2 cut(s)	1062, 1484
BstENI	CCTNNNNNAGG	1 cut(s)	1134
BstF5I	GGATG	6 cut(s)	161, 186, 259, 1084, 1324, 2353
BstFNI	CGCG	2 cut(s)	66, 1609
BstH2I	RGCGCY	1 cut(s)	2541
BstHHI	GCGC	4 cut(s)	1609, 1611, 2540, 2548
BstMAI	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
BstMWI	GCNNNNNNNGC	7 cut(s)	98, 888, 1130, 1604, 2062, 2537, 2557
BstNI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
BstNSI	RCATGY	4 cut(s)	481, 1423, 1641, 2371
BstSCI	CCNGG	8 cut(s)	288, 1140, 1155, 1164, 1692, 1810, 2187, 2466
BstSFI	CTRYAG	2 cut(s)	1962, 2541
BstSLI	GKGCMC	1 cut(s)	217
BstUI	CGCG	2 cut(s)	66, 1609
BstV1I	GCAGC	6 cut(s)	1623, 1794, 1948, 2527, 2555, 2560
BstV2I	GAAGAC	2 cut(s)	2370, 2478
BstX2I	RGATCY	4 cut(s)	496, 1624, 1708, 2143
BstYI	RGATCY	4 cut(s)	496, 1624, 1708, 2143
Bsu36I	CCTNAGG	1 cut(s)	2256
BsuI	GTATCC	4 cut(s)	848, 906, 1214, 1441
BtgI	CCRYGG	2 cut(s)	1062, 1484
BtsCI	GGATG	6 cut(s)	161, 186, 259, 1084, 1324, 2353
BtsIMutI	CAGTG	4 cut(s)	882, 1191, 2220, 2430
Cac8I	GCNNGC	5 cut(s)	531, 1421, 1609, 2428, 2508
CaiI	CAGNNNCTG	4 cut(s)	956, 1844, 2219, 2506
CciI	TCATGA	1 cut(s)	610
CfoI	GCGC	4 cut(s)	1609, 1611, 2540, 2548
Cfr13I	GGNCC	6 cut(s)	12, 213, 214, 1162, 1761, 2355
CseI	GACGC	1 cut(s)	382
Csp6I	GTAC	3 cut(s)	241, 2461, 2564
CspCI	CAANNNNNGTGG	2 cut(s)	440, 475
CviAII	CATG	9 cut(s)	437, 478, 611, 1063, 1420, 1589, 1638, 1970, 2368
CviQI	GTAC	3 cut(s)	241, 2461, 2564
DraI	TTTAAA	1 cut(s)	1036
DraIII	CACNNNGTG	1 cut(s)	1463
EaeI	YGGCCR	4 cut(s)	61, 1059, 2508, 2529
Eam1104I	CTCTTC	3 cut(s)	68, 644, 1184
EarI	CTCTTC	3 cut(s)	68, 644, 1184
Eco130I	CCWWGG	2 cut(s)	1062, 1360
Eco147I	AGGCCT	1 cut(s)	2274
Eco24I	GRGCYC	2 cut(s)	217, 867
Eco31I	GGTCTC	2 cut(s)	525, 2096
Eco47I	GGWCC	4 cut(s)	12, 1162, 1761, 2355
Eco47III	AGCGCT	1 cut(s)	2539
Eco57I	CTGAAG	2 cut(s)	1238, 2436
Eco81I	CCTNAGG	1 cut(s)	2256
Eco88I	CYCGRG	2 cut(s)	84, 866
EcoNI	CCTNNNNNAGG	1 cut(s)	1134
EcoO109I	RGGNCCY	1 cut(s)	12
EcoRI	GAATTC	1 cut(s)	757
EcoRII	CCWGG	4 cut(s)	288, 1164, 2187, 2466
EcoT14I	CCWWGG	2 cut(s)	1062, 1360
EcoT38I	GRGCYC	2 cut(s)	217, 867
ErhI	CCWWGG	2 cut(s)	1062, 1360
Esp3I	CGTCTC	2 cut(s)	219, 2294
FaeI	CATG	9 cut(s)	440, 481, 614, 1066, 1423, 1592, 1641, 1973, 2371
FalI	AAGNNNNNCTT	2 cut(s)	2304, 2336
FaqI	GGGAC	2 cut(s)	380, 2408
FatI	CATG	9 cut(s)	436, 477, 610, 1062, 1419, 1588, 1637, 1969, 2367
FauI	CCCGC	1 cut(s)	1257
FauNDI	CATATG	1 cut(s)	1650
FbaI	TGATCA	1 cut(s)	183
Fnu4HI	GCNGC	8 cut(s)	64, 1357, 1612, 1808, 1962, 2541, 2544, 2549
FokI	GGATG	6 cut(s)	148, 193, 266, 1071, 1331, 2360
FriOI	GRGCYC	2 cut(s)	217, 867
Fsp4HI	GCNGC	8 cut(s)	64, 1357, 1612, 1808, 1962, 2541, 2544, 2549
FspBI	CTAG	5 cut(s)	21, 795, 1376, 1424, 2180
GlaI	GCGC	4 cut(s)	1608, 1610, 2539, 2547
GluI	GCNGC	8 cut(s)	64, 1357, 1612, 1808, 1962, 2541, 2544, 2549
GsaI	CCCAGC	3 cut(s)	253, 1669, 2451
GsuI	CTGGAG	1 cut(s)	123
HaeII	RGCGCY	1 cut(s)	2541
HapII	CCGG	5 cut(s)	1141, 1156, 1693, 1811, 2408
HgaI	GACGC	1 cut(s)	382
HhaI	GCGC	4 cut(s)	1609, 1611, 2540, 2548
Hin1II	CATG	9 cut(s)	440, 481, 614, 1066, 1423, 1592, 1641, 1973, 2371
Hin6I	GCGC	4 cut(s)	1607, 1609, 2538, 2546
HinP1I	GCGC	4 cut(s)	1607, 1609, 2538, 2546
HincII	GTYRAC	3 cut(s)	1082, 1480, 2266
HindII	GTYRAC	3 cut(s)	1082, 1480, 2266
HindIII	AAGCTT	3 cut(s)	531, 1724, 1949
HpaII	CCGG	5 cut(s)	1141, 1156, 1693, 1811, 2408
HphI	GGTGA	5 cut(s)	56, 483, 1324, 1708, 2177
Hpy166II	GTNNAC	5 cut(s)	449, 547, 1082, 1480, 2266
Hpy8I	GTNNAC	5 cut(s)	449, 547, 1082, 1480, 2266
Hpy99I	CGWCG	1 cut(s)	2363
HpyAV	CCTTC	5 cut(s)	708, 749, 1206, 1211, 1653
HpyCH4III	ACNGT	7 cut(s)	97, 134, 886, 1003, 1186, 1551, 2434
HpyCH4IV	ACGT	1 cut(s)	2287
HpyF10VI	GCNNNNNNNGC	7 cut(s)	98, 888, 1130, 1604, 2062, 2537, 2557
HpySE526I	ACGT	1 cut(s)	2287
Hsp92II	CATG	9 cut(s)	440, 481, 614, 1066, 1423, 1592, 1641, 1973, 2371
HspAI	GCGC	4 cut(s)	1607, 1609, 2538, 2546
Ksp22I	TGATCA	1 cut(s)	183
LmnI	GCTCC	4 cut(s)	160, 933, 2173, 2521
Lsp1109I	GCAGC	6 cut(s)	1623, 1794, 1948, 2527, 2555, 2560
LweI	GCATC	4 cut(s)	142, 1168, 1486, 1794
MaeI	CTAG	5 cut(s)	21, 795, 1376, 1424, 2180
MaeII	ACGT	1 cut(s)	2287
MaeIII	GTNAC	3 cut(s)	44, 1303, 2496
MfeI	CAATTG	1 cut(s)	1070
MflI	RGATCY	4 cut(s)	496, 1624, 1708, 2143
MhlI	GDGCHC	5 cut(s)	217, 751, 867, 1451, 2119
MlsI	TGGCCA	2 cut(s)	1061, 2510
MluNI	TGGCCA	2 cut(s)	1061, 2510
MlyI	GAGTC	3 cut(s)	41, 1485, 2390
MmeI	TCCRAC	2 cut(s)	1828, 2382
Mox20I	TGGCCA	2 cut(s)	1061, 2510
MroXI	GAANNNNTTC	2 cut(s)	1726, 2196
MscI	TGGCCA	2 cut(s)	1061, 2510
MseI	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
MslI	CAYNNNNRTG	1 cut(s)	1424
Msp20I	TGGCCA	2 cut(s)	1061, 2510
MspA1I	CMGCKG	1 cut(s)	1310
MspI	CCGG	5 cut(s)	1141, 1156, 1693, 1811, 2408
MspR9I	CCNGG	8 cut(s)	290, 1142, 1157, 1166, 1694, 1812, 2189, 2468
MunI	CAATTG	1 cut(s)	1070
Mva1269I	GAATGC	1 cut(s)	828
MvaI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
MvnI	CGCG	2 cut(s)	66, 1609
MwoI	GCNNNNNNNGC	7 cut(s)	98, 888, 1130, 1604, 2062, 2537, 2557
NciI	CCSGG	4 cut(s)	1142, 1157, 1694, 1812
NcoI	CCATGG	1 cut(s)	1062
NdeI	CATATG	1 cut(s)	1650
NlaIII	CATG	9 cut(s)	440, 481, 614, 1066, 1423, 1592, 1641, 1973, 2371
NlaIV	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
NmeAIII	GCCGAG	1 cut(s)	997
NmuCI	GTSAC	1 cut(s)	44
NspI	RCATGY	4 cut(s)	481, 1423, 1641, 2371
PaeI	GCATGC	1 cut(s)	1423
PaeR7I	CTCGAG	1 cut(s)	866
PagI	TCATGA	1 cut(s)	610
PauI	GCGCGC	1 cut(s)	1607
PceI	AGGCCT	1 cut(s)	2274
PciI	ACATGT	1 cut(s)	2367
PctI	GAATGC	1 cut(s)	828
PdmI	GAANNNNTTC	2 cut(s)	1726, 2196
PflMI	CCANNNNNTGG	4 cut(s)	59, 500, 625, 2153
PfoI	TCCNGGA	1 cut(s)	1155
PkrI	GCNGC	8 cut(s)	65, 1358, 1613, 1809, 1963, 2542, 2545, 2550
PleI	GAGTC	3 cut(s)	41, 1484, 2390
PpsI	GAGTC	3 cut(s)	41, 1484, 2390
PpuMI	RGGWCCY	1 cut(s)	12
PscI	ACATGT	1 cut(s)	2367
PsiI	TTATAA	1 cut(s)	1755
Psp5II	RGGWCCY	1 cut(s)	12
Psp6I	CCWGG	4 cut(s)	288, 1164, 2187, 2466
PspFI	CCCAGC	3 cut(s)	249, 1665, 2447
PspGI	CCWGG	4 cut(s)	288, 1164, 2187, 2466
PspN4I	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
PspOMI	GGGCCC	1 cut(s)	213
PspPI	GGNCC	6 cut(s)	12, 213, 214, 1162, 1761, 2355
PspPPI	RGGWCCY	1 cut(s)	12
PspXI	VCTCGAGB	1 cut(s)	866
PstI	CTGCAG	2 cut(s)	1966, 2545
PstNI	CAGNNNCTG	4 cut(s)	956, 1844, 2219, 2506
PsuI	RGATCY	4 cut(s)	496, 1624, 1708, 2143
PteI	GCGCGC	1 cut(s)	1607
RsaI	GTAC	3 cut(s)	242, 2462, 2565
RsaNI	GTAC	3 cut(s)	241, 2461, 2564
RseI	CAYNNNNRTG	1 cut(s)	1424
SaqAI	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
SatI	GCNGC	8 cut(s)	64, 1357, 1612, 1808, 1962, 2541, 2544, 2549
Sau96I	GGNCC	6 cut(s)	12, 213, 214, 1162, 1761, 2355
ScaI	AGTACT	2 cut(s)	2462, 2565
SchI	GAGTC	3 cut(s)	41, 1485, 2390
ScrFI	CCNGG	8 cut(s)	290, 1142, 1157, 1166, 1694, 1812, 2189, 2468
SduI	GDGCHC	5 cut(s)	217, 751, 867, 1451, 2119
SfaNI	GCATC	4 cut(s)	142, 1168, 1486, 1794
SfcI	CTRYAG	2 cut(s)	1962, 2541
Sfr274I	CTCGAG	1 cut(s)	866
SinI	GGWCC	4 cut(s)	12, 1162, 1761, 2355
SlaI	CTCGAG	1 cut(s)	866
SmiMI	CAYNNNNRTG	1 cut(s)	1424
SmlI	CTYRAG	2 cut(s)	171, 866
SmoI	CTYRAG	2 cut(s)	171, 866
SphI	GCATGC	1 cut(s)	1423
SseBI	AGGCCT	1 cut(s)	2274
SsiI	CCGC	5 cut(s)	64, 1250, 1289, 1310, 1357
SspMI	CTAG	5 cut(s)	21, 795, 1376, 1424, 2180
StuI	AGGCCT	1 cut(s)	2274
StyD4I	CCNGG	8 cut(s)	288, 1140, 1155, 1164, 1692, 1810, 2187, 2466
StyI	CCWWGG	2 cut(s)	1062, 1360
TaaI	ACNGT	7 cut(s)	97, 134, 886, 1003, 1186, 1551, 2434
TaiI	ACGT	1 cut(s)	2290
TaqI	TCGA	2 cut(s)	867, 1771
TaqII	GACCGA	2 cut(s)	507, 2426
TatI	WGTACW	2 cut(s)	2460, 2563
TauI	GCSGC	2 cut(s)	66, 1359
Tru1I	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
Tru9I	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
TscAI	CASTG	4 cut(s)	889, 1191, 2220, 2437
TseFI	GTSAC	1 cut(s)	44
TseI	GCWGC	6 cut(s)	1611, 1807, 1961, 2540, 2543, 2548
Tsp45I	GTSAC	1 cut(s)	44
TspDTI	ATGAA	5 cut(s)	461, 599, 825, 1340, 2318
TspGWI	ACGGA	3 cut(s)	147, 193, 532
TspRI	CASTG	4 cut(s)	889, 1191, 2220, 2437
Van91I	CCANNNNNTGG	4 cut(s)	59, 500, 625, 2153
VpaK11BI	GGWCC	4 cut(s)	12, 1162, 1761, 2355
XagI	CCTNNNNNAGG	1 cut(s)	1134
XapI	RAATTY	3 cut(s)	428, 757, 1714
XbaI	TCTAGA	1 cut(s)	2179
XceI	RCATGY	4 cut(s)	481, 1423, 1641, 2371
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2034, 2464
XhoI	CTCGAG	1 cut(s)	866
XmnI	GAANNNNTTC	2 cut(s)	1726, 2196
XspI	CTAG	5 cut(s)	21, 795, 1376, 1424, 2180
ZrmI	AGTACT	2 cut(s)	2462, 2565

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.