Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003712

Physical Location & Seq

Genomic Coordinates Reverse (-)

22327210 .. 22331376

Gene Structure 4167 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG248600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGTTTCGAGATCTTATTCCAACTTACTTGACCTTACTTCTGGTGAATCCCTAACTTTTGGCCGTGAGAAGAAGAGGTTCCCGCGAGTTGCTACTGTTGCGGGAGTATTGTCTGAGCTAGATGATGATACCAGTAACAACAGTGTTGGCTCTGATGCTCCTTCGTCCATTTCTCAAGACCGGATGATCATAGTAGGGAATCAGCTTCCACTTCGGGCGCATAGAAGCGATGATGGGGAATGGTGCTTTAGCTGGGATGAGGATTCACTACTCTTGCAACTCAAAGATGGCCTTGGAGAAGATGTAGAAGTTATCTATATTGGTTGTCTAAAAGAAGAAATTGACCCGAGTGAGCAAGAGGATGTAGCCCAAACTTTGCTTGATACTTTTAAATGTGTCCCTGCATTTATCCCTCCAAACCTTTTCAGTAAATTTTATCATGGCTTCTGTAAGCAGCATTTGTGGCCTTTGTTTCACTACATGCTTCCTCTATCACCGGATCTCGGTGGCAGGTTTGATCGATCGCTGTGGCAAGCTTATGTTTCTGTGAACAAGATATTTGCAGATAAAGTGATGGAAGTTATTAGCCCGGATGAAGATTTTGTGTGGGTTCATGACTACCATTTGATGGTTTTGCCAACATTCTTGAGGAAGAGGTTTAATAGGTTGAAGCTGGGATTCTTTCTCCATAGTCCATTCCCTTCGTCTGAGATATACAGAACACTTCCAGTAAGGGATGACCTTCTAAGAGCACTTCTAAATTCTGATCTCATTGGCTTCCATACTTTTGATTATGCTAGGCACTTCCTTTCTTGTTGTAGTAGAATGCTTGGCCTCTCATATCAATCGAAGAGGGGATACATTGGGCTCGAGTATTATGGCCGCACAGTAAGCATTAAGATTCTTCCTGTTGGGATACATATAGGCCAGCTTCAATCTGTGTTGAATCTGCCAGAGACTGAATCTAAGGTTGCTGAGCTGCAAGATCAATTTAGGGGGAAAACTGTTTTGCTTGGGGTTGATGACATGGACATCTTTAAGGGAATTAGCTTGAAACTTTTGGCTATGGAGCAATTGCTTATACAACATCCTGATAAGAGGGGTAAAGTTGTTCTTATTCAAATTGCTAACCCGGCAAGGGGCCGGGGGAAAGATGTCCTGGAGGTCCAGTTAGAAACTGATGCCACAGTGAAGAGGATCAATGAGACATTTGGGAAGCAAGGATATAGTCCAGTGGTCTTGATCGATACCCCACTTCAGTTTTTTGAGCGAATTGCTTATTATGTTATTGCAGAGTGTTGTCTTGTTACAGCAGTGAGGGACGGGATGAATCTTATACCCTATGAGTACATAATATGCCGCCAAGGAAATGAGAAGCTGAATGAGACTTTAGGACTAAACCCAACAACCCCAAAGAAAAGCATGCTTGTGGTTTCTGAGTTTATTGGGTGTTCTCCATCACTAAGCGGAGCAATTCGAGTTAACCCATGGAACATTGATGCTGTAGTTGAAGCTATGGATTCTGCTCTTATGATCCCAGAAGGAGAAAAACAGTTGCGGCATGAGAAGCATTATAGGTATGTCAGTACACATGATGTTGCATATTGGGCACGTAGTTTCTTGCAGGATCTTGAAAGGGCATGTAAGGATCACATGAGGAGGAGATGCTGGGGAATTGGTTTTGGTTTAGGGTTCAGGGTAATTGCTTTGGATCCAAATTTCAGAAAGCTTTCAGTTGAGCATATTGTTTCAGCTTACAAGAGGACGAAATGTCGAGCAATTCTCTTGGATTATGATGGCGCTATGATGCTGCCGGGTTCAATTATTACCACACCTAATTCAGAGGCTATTGGAATCTTGAACAATTTATGCAATGACCCGAGGAATGTTGTATTTCTTGTTAGTGGGAAAGACAGGAAGACTTTAACTGAATGGTTCTCTTCTTGTGGGAAGCTTGGGATTGCAGCAGAGCATGGCTTTTTTGTGAGGACAAATCATAATGCTGATTGGGAAACGTGCTTCTCTGTTCCTGATTTTGATTGGAAACAGATTGCTGAACCAGTAATGCAGTTATACACTGAAACAACTGATGGTTCTAATATAGAGGCTAAAGAAAGTGCTCTTGTTTGGAATTACCAGTATGCAGATCCAGATTTTGGCTTCTGTCAGGCTAAGGAGCTTCTGGATCACCTGGAAAGTGTTCTTGCCAATGAGCCAGTTTCTGTCAAAAGTGGCCAGCACATTGTAGAAGTTAAACCTCAGGGTGTCAACAAGGGCCTTGTGGCAGAACGCCTCCTCTTAACAATGAAACAGAAGGCAATGCTCCCAGACTTTGTTCTCTGCATTGGGGATGACAGGTCCGATGAAGACATGTTTGAAGTGATAATGAGTGCAAGGGACTCTCTCTCTCCAGTCTCTGAAGTGTTTGCTTGCACTGTTGGTCGGAAGCCCAGCAAGGCCAAGTACTTCTTGGAAGACACAACTGAGATTGTGAGAATGTTACAGGTCCTTGCCAATGCTTCAGAGCAGGCGGCTAGAAGTACCCCACAACCTTCTCAGCGAGTGGTTACTGTAGAAAGCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

861

Amino Acids

97.31

Weight (kDa)

5.71

Isoelectric Point (pI)

45.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	61 - 545	2.5e-187	Glycosyltransferase family 20
Trehalose_PPase	PF02358	595 - 827	1.4e-80	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17770 AT4G17770
fragaria_vesca	FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550
malus_domestica	MD06G1037500.v1.1
prunus_persica	Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1
pyrus_communis	pycom06g03100
rosa_chinensis	RchiOBHm_Chr7g0210321
rosa_laevigata	RLG00000003046
rosa_multiflora	Rmu_sc0004503.1_g000003 Rmu_sc0017069.1_g000008
rosa_roxburghii	Rroxscaffold_3G00248400
rosa_rugosa	Rorug07G0120600 Rorug07G0120700 Rorug07G0120800 Rorug07G0120800 Rorug07G0120900 Rorug07G0121000 Rorug07G0121000 Rorug07G0121000
rosa_samantha	Rh7BG248600
rosa_wichuraiana	Rw7G021630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	501
AccB7I	CCANNNNNTGG	2 cut(s)	628, 2156
AccII	CGCG	1 cut(s)	85
AciI	CCGC	7 cut(s)	83, 101, 883, 1360, 1467, 1558, 2531
AclWI	GGATC	9 cut(s)	507, 1205, 1528, 1635, 1656, 1706, 1719, 2141, 2193
AcoI	YGGCCR	3 cut(s)	61, 880, 2233
AcsI	RAATTY	3 cut(s)	431, 760, 1717
AcuI	CTGAAG	3 cut(s)	1241, 2439, 2505
AfaI	GTAC	4 cut(s)	1349, 1588, 2465, 2542
AfiI	CCNNNNNNNGG	5 cut(s)	503, 628, 1138, 1139, 2156
AflIII	ACRYGT	1 cut(s)	2370
AhdI	GACNNNNNGTC	1 cut(s)	1770
AjnI	CCWGG	2 cut(s)	1158, 2190
Alw21I	GWGCWC	2 cut(s)	754, 2122
Alw26I	GTCTC	4 cut(s)	950, 1199, 1379, 2419
AlwI	GGATC	9 cut(s)	507, 1205, 1528, 1635, 1656, 1706, 1719, 2141, 2193
AlwNI	CAGNNNCTG	3 cut(s)	959, 2222, 2417
Ama87I	CYCGRG	3 cut(s)	346, 869, 1879
ApeKI	GCWGC	4 cut(s)	454, 979, 1810, 1964
ApoI	RAATTY	3 cut(s)	431, 760, 1717
Asp700I	GAANNNNTTC	3 cut(s)	77, 1729, 2199
AspLEI	GCGC	2 cut(s)	220, 1802
AspS9I	GGNCC	5 cut(s)	1141, 1165, 2275, 2358, 2506
AsuC2I	CCSGG	4 cut(s)	590, 1133, 1145, 1815
AsuHPI	GGTGA	3 cut(s)	56, 486, 2180
AvaI	CYCGRG	3 cut(s)	346, 869, 1879
AvaII	GGWCC	3 cut(s)	1165, 2358, 2506
AxyI	CCTNAGG	1 cut(s)	2259
BaeGI	GKGCMC	1 cut(s)	1612
BalI	TGGCCA	1 cut(s)	2235
BamHI	GGATCC	1 cut(s)	1711
BanII	GRGCYC	1 cut(s)	870
BarI	GAAGNNNNNNTAC	2 cut(s)	842, 874
BbsI	GAAGAC	3 cut(s)	1925, 2373, 2481
Bbv12I	GWGCWC	2 cut(s)	754, 2122
BbvI	GCAGC	4 cut(s)	466, 966, 1797, 1976
BccI	CCATC	7 cut(s)	227, 281, 568, 622, 1465, 1790, 2084
BceAI	ACGGC	1 cut(s)	48
BciT130I	CCWGG	2 cut(s)	1160, 2192
BciVI	GTATCC	2 cut(s)	851, 909
BclI	TGATCA	1 cut(s)	186
BcnI	CCSGG	4 cut(s)	590, 1133, 1145, 1815
BcoDI	GTCTC	4 cut(s)	950, 1199, 1379, 2419
BfaI	CTAG	3 cut(s)	119, 798, 2535
BfmI	CTRYAG	2 cut(s)	1503, 2571
BfoI	RGCGCY	1 cut(s)	1803
BfuAI	ACCTGC	1 cut(s)	501
BfuI	GTATCC	2 cut(s)	851, 909
BglII	AGATCT	1 cut(s)	11
BisI	GCNGC	8 cut(s)	455, 883, 980, 1360, 1559, 1811, 1965, 2532
BlpI	GCTNAGC	1 cut(s)	975
BlsI	GCNGC	8 cut(s)	456, 884, 981, 1361, 1560, 1812, 1966, 2533
BmcAI	AGTACT	1 cut(s)	2465
Bme1390I	CCNGG	6 cut(s)	590, 1133, 1145, 1160, 1815, 2192
Bme18I	GGWCC	3 cut(s)	1165, 2358, 2506
BmeRI	GACNNNNNGTC	1 cut(s)	1770
BmeT110I	CYCGRG	3 cut(s)	346, 869, 1879
BmgT120I	GGNCC	5 cut(s)	1141, 1165, 2275, 2358, 2506
BmiI	GGNNCC	3 cut(s)	80, 1142, 1713
BmrFI	CCNGG	6 cut(s)	590, 1133, 1145, 1160, 1815, 2192
BmsI	GCATC	5 cut(s)	145, 1171, 1489, 1655, 1797
BpiI	GAAGAC	3 cut(s)	1925, 2373, 2481
BplI	GAGNNNNNCTC	2 cut(s)	1767, 1799
BpmI	CTGGAG	2 cut(s)	1181, 2394
Bpu10I	CCTNAGC	1 cut(s)	2172
Bpu1102I	GCTNAGC	1 cut(s)	975
BpuEI	CTTGAG	2 cut(s)	159, 667
BpuMI	CCSGG	4 cut(s)	590, 1133, 1145, 1815
Bsa29I	ATCGAT	2 cut(s)	520, 1245
BsaAI	YACGTR	1 cut(s)	1613
BsaJI	CCNNGG	5 cut(s)	292, 1144, 1363, 1487, 1880
BsaWI	WCCGGW	2 cut(s)	180, 496
BsaXI	ACNNNNNCTCC	4 cut(s)	288, 318, 1154, 1184
Bsc4I	CCNNNNNNNGG	5 cut(s)	503, 628, 1138, 1139, 2156
Bse1I	ACTGG	8 cut(s)	132, 728, 1168, 1232, 2060, 2137, 2216, 2411
Bse21I	CCTNAGG	1 cut(s)	2259
Bse3DI	GCAATG	2 cut(s)	1879, 2325
BseBI	CCWGG	2 cut(s)	1160, 2192
BseCI	ATCGAT	2 cut(s)	520, 1245
BseDI	CCNNGG	5 cut(s)	292, 1144, 1363, 1487, 1880
BseGI	GGATG	8 cut(s)	189, 262, 367, 598, 742, 1087, 1332, 2356
BseLI	CCNNNNNNNGG	5 cut(s)	503, 628, 1138, 1139, 2156
BseMI	GCAATG	2 cut(s)	1879, 2325
BseMII	CTCAG	7 cut(s)	105, 699, 966, 1428, 2273, 2475, 2570
BseNI	ACTGG	8 cut(s)	132, 728, 1168, 1232, 2060, 2137, 2216, 2411
BseRI	GAGGAG	3 cut(s)	1672, 1675, 2285
BseSI	GKGCMC	1 cut(s)	1612
BseXI	GCAGC	4 cut(s)	466, 966, 1797, 1976
BseYI	CCCAGC	4 cut(s)	252, 673, 1668, 2450
Bsh1236I	CGCG	1 cut(s)	85
Bsh1285I	CGRYCG	1 cut(s)	524
BshVI	ATCGAT	2 cut(s)	520, 1245
BsiEI	CGRYCG	1 cut(s)	524
BsiHKAI	GWGCWC	2 cut(s)	754, 2122
BsiHKCI	CYCGRG	3 cut(s)	346, 869, 1879
BsiSI	CCGG	6 cut(s)	181, 497, 590, 1133, 1144, 1814
BslFI	GGGAC	3 cut(s)	383, 1334, 2411
BslI	CCNNNNNNNGG	5 cut(s)	503, 628, 1138, 1139, 2156
BsmAI	GTCTC	4 cut(s)	950, 1199, 1379, 2419
BsmFI	GGGAC	3 cut(s)	383, 1334, 2411
BsmI	GAATGC	1 cut(s)	831
BsoBI	CYCGRG	3 cut(s)	346, 869, 1879
Bsp1286I	GDGCHC	4 cut(s)	754, 870, 1612, 2122
Bsp1720I	GCTNAGC	1 cut(s)	975
Bsp19I	CCATGG	1 cut(s)	1487
BspACI	CCGC	7 cut(s)	83, 101, 883, 1360, 1467, 1558, 2531
BspCNI	CTCAG	7 cut(s)	106, 700, 967, 1429, 2272, 2476, 2569
BspDI	ATCGAT	2 cut(s)	520, 1245
BspFNI	CGCG	1 cut(s)	85
BspHI	TCATGA	1 cut(s)	613
BspLI	GGNNCC	3 cut(s)	80, 1142, 1713
BspMI	ACCTGC	1 cut(s)	501
BspPI	GGATC	9 cut(s)	507, 1205, 1528, 1635, 1656, 1706, 1719, 2141, 2193
BsrDI	GCAATG	2 cut(s)	1879, 2325
BsrI	ACTGG	8 cut(s)	132, 728, 1168, 1232, 2060, 2137, 2216, 2411
BssECI	CCNNGG	5 cut(s)	292, 1144, 1363, 1487, 1880
BssT1I	CCWWGG	3 cut(s)	292, 1363, 1487
Bst2UI	CCWGG	2 cut(s)	1160, 2192
Bst4CI	ACNGT	8 cut(s)	97, 143, 889, 1006, 1189, 1554, 2437, 2572
Bst6I	CTCTTC	5 cut(s)	68, 647, 845, 1187, 1945
BstBAI	YACGTR	1 cut(s)	1613
BstC8I	GCNNGC	6 cut(s)	534, 929, 1424, 2237, 2431, 2529
BstDSI	CCRYGG	1 cut(s)	1487
BstF5I	GGATG	8 cut(s)	189, 262, 367, 598, 742, 1087, 1332, 2356
BstFNI	CGCG	1 cut(s)	85
BstH2I	RGCGCY	1 cut(s)	1803
BstHHI	GCGC	2 cut(s)	220, 1802
BstMAI	GTCTC	4 cut(s)	950, 1199, 1379, 2419
BstMCI	CGRYCG	1 cut(s)	524
BstMWI	GCNNNNNNNGC	6 cut(s)	98, 463, 891, 1133, 1567, 1607
BstNI	CCWGG	2 cut(s)	1160, 2192
BstNSI	RCATGY	4 cut(s)	484, 1426, 1644, 2374
BstSCI	CCNGG	6 cut(s)	588, 1131, 1143, 1158, 1813, 2190
BstSFI	CTRYAG	2 cut(s)	1503, 2571
BstSLI	GKGCMC	1 cut(s)	1612
BstUI	CGCG	1 cut(s)	85
BstV1I	GCAGC	4 cut(s)	466, 966, 1797, 1976
BstV2I	GAAGAC	3 cut(s)	1925, 2373, 2481
BstX2I	RGATCY	5 cut(s)	11, 499, 1627, 1711, 2146
BstYI	RGATCY	5 cut(s)	11, 499, 1627, 1711, 2146
Bsu15I	ATCGAT	2 cut(s)	520, 1245
Bsu36I	CCTNAGG	1 cut(s)	2259
BsuI	GTATCC	2 cut(s)	851, 909
BsuTUI	ATCGAT	2 cut(s)	520, 1245
BtgI	CCRYGG	1 cut(s)	1487
BtgZI	GCGATG	1 cut(s)	243
BtsCI	GGATG	8 cut(s)	189, 262, 367, 598, 742, 1087, 1332, 2356
BtsI	GCAGTG	1 cut(s)	1320
BtsIMutI	CAGTG	6 cut(s)	148, 1194, 1239, 1320, 2076, 2433
BveI	ACCTGC	1 cut(s)	501
Cac8I	GCNNGC	6 cut(s)	534, 929, 1424, 2237, 2431, 2529
CaiI	CAGNNNCTG	3 cut(s)	959, 2222, 2417
CciI	TCATGA	1 cut(s)	613
CfoI	GCGC	2 cut(s)	220, 1802
Cfr13I	GGNCC	5 cut(s)	1141, 1165, 2275, 2358, 2506
ClaI	ATCGAT	2 cut(s)	520, 1245
Csp6I	GTAC	4 cut(s)	1348, 1587, 2464, 2541
CviQI	GTAC	4 cut(s)	1348, 1587, 2464, 2541
DraI	TTTAAA	1 cut(s)	391
DriI	GACNNNNNGTC	1 cut(s)	1770
EaeI	YGGCCR	3 cut(s)	61, 880, 2233
Eam1104I	CTCTTC	5 cut(s)	68, 647, 845, 1187, 1945
Eam1105I	GACNNNNNGTC	1 cut(s)	1770
EarI	CTCTTC	5 cut(s)	68, 647, 845, 1187, 1945
Eco130I	CCWWGG	3 cut(s)	292, 1363, 1487
Eco24I	GRGCYC	1 cut(s)	870
Eco47I	GGWCC	3 cut(s)	1165, 2358, 2506
Eco57I	CTGAAG	3 cut(s)	1241, 2439, 2505
Eco81I	CCTNAGG	1 cut(s)	2259
Eco88I	CYCGRG	3 cut(s)	346, 869, 1879
EcoO109I	RGGNCCY	2 cut(s)	2275, 2506
EcoRII	CCWGG	2 cut(s)	1158, 2190
EcoT14I	CCWWGG	3 cut(s)	292, 1363, 1487
EcoT38I	GRGCYC	1 cut(s)	870
ErhI	CCWWGG	3 cut(s)	292, 1363, 1487
FalI	AAGNNNNNCTT	6 cut(s)	276, 308, 1098, 1130, 2453, 2485
FaqI	GGGAC	3 cut(s)	383, 1334, 2411
FauI	CCCGC	2 cut(s)	90, 94
FbaI	TGATCA	1 cut(s)	186
Fnu4HI	GCNGC	8 cut(s)	455, 883, 980, 1360, 1559, 1811, 1965, 2532
FokI	GGATG	8 cut(s)	196, 269, 374, 605, 749, 1074, 1339, 2363
FriOI	GRGCYC	1 cut(s)	870
Fsp4HI	GCNGC	8 cut(s)	455, 883, 980, 1360, 1559, 1811, 1965, 2532
FspBI	CTAG	3 cut(s)	119, 798, 2535
GlaI	GCGC	2 cut(s)	219, 1801
GluI	GCNGC	8 cut(s)	455, 883, 980, 1360, 1559, 1811, 1965, 2532
GsaI	CCCAGC	4 cut(s)	256, 677, 1672, 2454
GsuI	CTGGAG	2 cut(s)	1181, 2394
HaeII	RGCGCY	1 cut(s)	1803
HapII	CCGG	6 cut(s)	181, 497, 590, 1133, 1144, 1814
HhaI	GCGC	2 cut(s)	220, 1802
Hin6I	GCGC	2 cut(s)	218, 1800
HinP1I	GCGC	2 cut(s)	218, 1800
HincII	GTYRAC	2 cut(s)	1483, 2269
HindII	GTYRAC	2 cut(s)	1483, 2269
HindIII	AAGCTT	3 cut(s)	534, 1727, 1952
HpaI	GTTAAC	1 cut(s)	1483
HpaII	CCGG	6 cut(s)	181, 497, 590, 1133, 1144, 1814
HphI	GGTGA	3 cut(s)	56, 486, 2180
Hpy166II	GTNNAC	4 cut(s)	550, 1483, 1589, 2269
Hpy8I	GTNNAC	4 cut(s)	550, 1483, 1589, 2269
HpyAV	CCTTC	6 cut(s)	171, 711, 752, 1535, 2308, 2562
HpyCH4III	ACNGT	8 cut(s)	97, 143, 889, 1006, 1189, 1554, 2437, 2572
HpyCH4IV	ACGT	2 cut(s)	1612, 2015
HpyF10VI	GCNNNNNNNGC	6 cut(s)	98, 463, 891, 1133, 1567, 1607
HpySE526I	ACGT	2 cut(s)	1612, 2015
HspAI	GCGC	2 cut(s)	218, 1800
Ksp22I	TGATCA	1 cut(s)	186
KspAI	GTTAAC	1 cut(s)	1483
LmnI	GCTCC	5 cut(s)	163, 1069, 1469, 2176, 2328
Lsp1109I	GCAGC	4 cut(s)	466, 966, 1797, 1976
LweI	GCATC	5 cut(s)	145, 1171, 1489, 1655, 1797
MaeI	CTAG	3 cut(s)	119, 798, 2535
MaeII	ACGT	2 cut(s)	1612, 2015
MaeIII	GTNAC	4 cut(s)	134, 1306, 2499, 2566
MfeI	CAATTG	1 cut(s)	1073
MflI	RGATCY	5 cut(s)	11, 499, 1627, 1711, 2146
MhlI	GDGCHC	4 cut(s)	754, 870, 1612, 2122
MlsI	TGGCCA	1 cut(s)	2235
MluNI	TGGCCA	1 cut(s)	2235
MlyI	GAGTC	1 cut(s)	2393
MmeI	TCCRAC	2 cut(s)	45, 2423
Mox20I	TGGCCA	1 cut(s)	2235
MroXI	GAANNNNTTC	3 cut(s)	77, 1729, 2199
MscI	TGGCCA	1 cut(s)	2235
MseI	TTAA	8 cut(s)	390, 660, 897, 1038, 1482, 1925, 2253, 2300
MslI	CAYNNNNRTG	1 cut(s)	1427
Msp20I	TGGCCA	1 cut(s)	2235
MspI	CCGG	6 cut(s)	181, 497, 590, 1133, 1144, 1814
MspR9I	CCNGG	6 cut(s)	590, 1133, 1145, 1160, 1815, 2192
MunI	CAATTG	1 cut(s)	1073
Mva1269I	GAATGC	1 cut(s)	831
MvaI	CCWGG	2 cut(s)	1160, 2192
MvnI	CGCG	1 cut(s)	85
MwoI	GCNNNNNNNGC	6 cut(s)	98, 463, 891, 1133, 1567, 1607
NciI	CCSGG	4 cut(s)	590, 1133, 1145, 1815
NcoI	CCATGG	1 cut(s)	1487
NlaIV	GGNNCC	3 cut(s)	80, 1142, 1713
NspI	RCATGY	4 cut(s)	484, 1426, 1644, 2374
PaeI	GCATGC	1 cut(s)	1426
PaeR7I	CTCGAG	1 cut(s)	869
PagI	TCATGA	1 cut(s)	613
PciI	ACATGT	1 cut(s)	2370
PctI	GAATGC	1 cut(s)	831
PdmI	GAANNNNTTC	3 cut(s)	77, 1729, 2199
PflMI	CCANNNNNTGG	2 cut(s)	628, 2156
PfoI	TCCNGGA	1 cut(s)	1158
PkrI	GCNGC	8 cut(s)	456, 884, 981, 1361, 1560, 1812, 1966, 2533
Ple19I	CGATCG	1 cut(s)	524
PleI	GAGTC	1 cut(s)	2393
PpsI	GAGTC	1 cut(s)	2393
Ppu21I	YACGTR	1 cut(s)	1613
PpuMI	RGGWCCY	1 cut(s)	2506
PscI	ACATGT	1 cut(s)	2370
Psp5II	RGGWCCY	1 cut(s)	2506
Psp6I	CCWGG	2 cut(s)	1158, 2190
PspFI	CCCAGC	4 cut(s)	252, 673, 1668, 2450
PspGI	CCWGG	2 cut(s)	1158, 2190
PspN4I	GGNNCC	3 cut(s)	80, 1142, 1713
PspPI	GGNCC	5 cut(s)	1141, 1165, 2275, 2358, 2506
PspPPI	RGGWCCY	1 cut(s)	2506
PspXI	VCTCGAGB	1 cut(s)	869
PstNI	CAGNNNCTG	3 cut(s)	959, 2222, 2417
PsuI	RGATCY	5 cut(s)	11, 499, 1627, 1711, 2146
PvuI	CGATCG	1 cut(s)	524
RsaI	GTAC	4 cut(s)	1349, 1588, 2465, 2542
RsaNI	GTAC	4 cut(s)	1348, 1587, 2464, 2541
RseI	CAYNNNNRTG	1 cut(s)	1427
SaqAI	TTAA	8 cut(s)	390, 660, 897, 1038, 1482, 1925, 2253, 2300
SatI	GCNGC	8 cut(s)	455, 883, 980, 1360, 1559, 1811, 1965, 2532
Sau96I	GGNCC	5 cut(s)	1141, 1165, 2275, 2358, 2506
ScaI	AGTACT	1 cut(s)	2465
SchI	GAGTC	1 cut(s)	2393
ScrFI	CCNGG	6 cut(s)	590, 1133, 1145, 1160, 1815, 2192
SduI	GDGCHC	4 cut(s)	754, 870, 1612, 2122
SfaNI	GCATC	5 cut(s)	145, 1171, 1489, 1655, 1797
SfcI	CTRYAG	2 cut(s)	1503, 2571
Sfr274I	CTCGAG	1 cut(s)	869
SinI	GGWCC	3 cut(s)	1165, 2358, 2506
SlaI	CTCGAG	1 cut(s)	869
SmiMI	CAYNNNNRTG	1 cut(s)	1427
SmlI	CTYRAG	3 cut(s)	174, 646, 869
SmoI	CTYRAG	3 cut(s)	174, 646, 869
SphI	GCATGC	1 cut(s)	1426
SsiI	CCGC	7 cut(s)	83, 101, 883, 1360, 1467, 1558, 2531
SspMI	CTAG	3 cut(s)	119, 798, 2535
StyD4I	CCNGG	6 cut(s)	588, 1131, 1143, 1158, 1813, 2190
StyI	CCWWGG	3 cut(s)	292, 1363, 1487
TaaI	ACNGT	8 cut(s)	97, 143, 889, 1006, 1189, 1554, 2437, 2572
TaiI	ACGT	2 cut(s)	1615, 2018
TaqI	TCGA	7 cut(s)	8, 520, 848, 870, 1245, 1477, 1774
TatI	WGTACW	3 cut(s)	1347, 1586, 2463
TauI	GCSGC	4 cut(s)	885, 1362, 1561, 2534
Tru1I	TTAA	8 cut(s)	390, 660, 897, 1038, 1482, 1925, 2253, 2300
Tru9I	TTAA	8 cut(s)	390, 660, 897, 1038, 1482, 1925, 2253, 2300
TscAI	CASTG	6 cut(s)	148, 1194, 1239, 1320, 2083, 2440
TseI	GCWGC	4 cut(s)	454, 979, 1810, 1964
TspDTI	ATGAA	5 cut(s)	602, 609, 1343, 2321, 2379
TspRI	CASTG	6 cut(s)	148, 1194, 1239, 1320, 2083, 2440
Van91I	CCANNNNNTGG	2 cut(s)	628, 2156
VpaK11BI	GGWCC	3 cut(s)	1165, 2358, 2506
XapI	RAATTY	3 cut(s)	431, 760, 1717
XceI	RCATGY	4 cut(s)	484, 1426, 1644, 2374
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2467
XhoI	CTCGAG	1 cut(s)	869
XmnI	GAANNNNTTC	3 cut(s)	77, 1729, 2199
XspI	CTAG	3 cut(s)	119, 798, 2535
ZrmI	AGTACT	1 cut(s)	2465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.