Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0003712

Physical Location & Seq

Genomic Coordinates Reverse (-)

3936085 .. 3941166

Gene Structure 5082 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g03100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGTTTCAAGGTCCTATTCTAGCTTACTTGACCTTACTTCTGGTGACTCCCCAACTTTTGGCCGCGAGAAGAAGAGGTTTCCCCGAGTGGCAACTGTCGCTGGAGTATTGTCTGAGTTTGATGATGACAACAGTAATAGCGTTGGCTCTGATGCTCCGTCATCCATTGCTCAAGAACGGATGATCATAGTGGGAAATCAGCTTCCACTTCGGGCCCATCGGAGAGACGATGGCGAATGGTACTTTAGCTGGGATGAGGATTCACTTCTTTTGCAACTCAAAGATGGCCTGGGAGAAGATGTAGAAGTAGTCTATATTGGTTGCCTTAAAGAAGAAATTGACCCAAGTGAGCAAGATGATGTAGCCCAAACTTTGCTTGATACTTTCAAATGCGTCCCTGCATTTATCCCACCTGACCTTTTTAGTAAATTTTATCATGGTTTTTGTAAACAACATTTGTGGCCTTTATTTCATTACATGCTTCCTCTCTCACCAGATCTTGGTGGTAGGTTTGATCGGTCTCTATGGCAAGCTTATGTTTCCGTGAACAAGATATTTGCAGATAAAGTAATGGAAGTGATTAGCCCTGATGATGATTTGGTGTGGGTTCATGACTACCATTTGATGGTGTTGCCAACATTTTTGAGGAAGAGGTTTAATAGATTGAGGCTTGGATTCTTTCTCCACAGCCCGTTCCCTTCGTCTGAGATATACAGGACACTTCCTGTAAGAGATGACCTTCTAAGAGCACTTCTGAATTCTGACCTTATTGGCTTCCATACTTTTGATTATGCTAGGCACTTCCTCTCTTGTTGTAGTAGAATGCTTGGCCTTTCATATCAATCTAAGCGAGGATACATCGGGCTTGAGTATTATGGTCGCACTGTAAGCATTAAGATTCTTCCTGTTGGGATACACTTACACCAGCTCCAAAATGTGTTGAATCTTCCAGAGACTGAATCTAAAGTTGCTGAGTTGCAAGATCAGTTTAGGGGGAAAACTGTTTTGCTTGGGGTTGATGATATGGACATATTTAAAGGAATCAGCTTGAAACTTTTGGCCATGGAACAATTGCTTATTCTACATCCTGATAAGAGGGGTAAGGTTGTTTTGGTTCAAGTTGCTAACCCTGCCAGAGGCCGGGGGAAAGATGTCCGAGAGGTCCAGGTAGAAACTGATGCCACAGTGAAGAGGATCAACGAGACATTTGGAAGGGAAGGATACAAACCTGTGGTCTTGATTGATACCCCGCTTCAGTTTTATGAGCGAATTGCTTATTATGCAATTGCGGAGTGTTGTCTTGTTACAGCGGTGAGGGATGGAATGAATCTTATACCCTATGAGTATATTATCTGCCGCCAAGGAAATGAGAAGCTAGATGAGACTTTAGGACTAAACCCAACAACCCCAAAGAAAAGCATGCTAGTGGTATCCGAGTTTATAGGATGCTCTCCATCACTAAGCGGGGCAATCCGAGTCAACCCATGGAACATTGATGCTGTTGCTGAAGCTATGGATTCTGCTCTTATGGTGCCAGAGGCAGAAAAACAGTTGCGACACGAGAAGCATTATAGGTATGTCATTACACATGATGTTGCATATTGGGCGCGCAGCTTCTTGCAGGATCTTGAAAGGGCATGTAGGGATCATATGAGGAGAAGGTGCTGGGGAATTGGTTTTGGCTTAGGGTTCCGGGTGATTGTTTTGGATCCAAATTTCAGAAAGCTTTCAGTTGAACTTATTGTTTCAGCTTATAAAAGGACCAAGAATCGAGCGATTCTTTTGGATTATGATGGTGCTATGATGCTGCCGGGTTCAATTATTACCACTCCTAATACAGAGGCTGTTGGAATCTTGAACAATTTATGCAATGATCCCAAGAATGTTGTCTTTCTTGTTAGTGGGAAAGACAGAAAGACTTTAACCAAGTGGTTTTCTTCTTGTGAAAAGCTTGGGATTGCAGCAGAGCATGGCTATTATGTGAGGTCAAATCATAATGCCGACTGGGAAACTTGTATCTCTATTCCAGATTTTGATTGGAAACAGATTGCTGAGCCAGTAATGCAGTTATATACCGAAACAACCGATGGTTCTACCATAGAGACCAAAGAAAGTGCTCTTGTTTGGAATTATCAATACGCAGATCCAGATTTTGGCTTTTGTCAGGCTAAGGAGCTTCTAGATCACCTGGAAAGTGTTCTTGCCAATGAACCAGTTTCTGTCAAGAGTGGTCAGCACATTGTAGAAGTTAAACCTCAGGGTGTCAACAAAGGCCTTGTAGCGGAACGTCTCCTCGTAGCAATGAAACAGAAAGCAATGCTTCCAGATTTTGTTCTGTGTATTGGGGATGACAGATCCGACGAAGACATGTTTGAGGCGATAATGAGTTCAAGGGACTCTCTCTCTCCGGTGGCTGAAGTGTTTGCTTGCACTGTCGGTCAGAAGCCCAGCAAGGCCAAGTACTACCTGGAAGACACAACTGAGATTCTGAGAATGTTACAGGGGCTAGCCAATGCTTCGGAGCAGGCGGCCAAAAGCGCTGCACCGCAACTTTCTCAGCGAGTGCTTACTTTAGAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

861

Amino Acids

97.38

Weight (kDa)

5.71

Isoelectric Point (pI)

45.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	60 - 544	1.1e-187	Glycosyltransferase family 20
Trehalose_PPase	PF02358	594 - 826	9.4e-80	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17770 AT4G17770
fragaria_vesca	FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550 FvH4_5g22550
malus_domestica	MD06G1037500.v1.1
prunus_persica	Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1 Prupe.5G031500_v2.0.a1
pyrus_communis	pycom06g03100
rosa_chinensis	RchiOBHm_Chr7g0210321
rosa_laevigata	RLG00000003046
rosa_multiflora	Rmu_sc0004503.1_g000003 Rmu_sc0017069.1_g000008
rosa_roxburghii	Rroxscaffold_3G00248400
rosa_rugosa	Rorug07G0120600 Rorug07G0120700 Rorug07G0120800 Rorug07G0120800 Rorug07G0120900 Rorug07G0121000 Rorug07G0121000 Rorug07G0121000
rosa_samantha	Rh7BG248600
rosa_wichuraiana	Rw7G021630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1755
AccB1I	GGYRCC	1 cut(s)	1531
AccB7I	CCANNNNNTGG	4 cut(s)	59, 500, 625, 2153
AccII	CGCG	2 cut(s)	66, 1609
AciI	CCGC	9 cut(s)	64, 1250, 1289, 1310, 1357, 1464, 2282, 2528, 2546
AclWI	GGATC	8 cut(s)	1202, 1632, 1653, 1703, 1716, 1868, 2138, 2349
AcoI	YGGCCR	3 cut(s)	61, 1059, 2529
AcsI	RAATTY	3 cut(s)	428, 757, 1714
AcuI	CTGAAG	3 cut(s)	1238, 1527, 2436
AfaI	GTAC	2 cut(s)	242, 2462
AfeI	AGCGCT	1 cut(s)	2539
AfiI	CCNNNNNNNGG	7 cut(s)	59, 500, 625, 1136, 1140, 2153, 2281
AflIII	ACRYGT	1 cut(s)	2367
AjnI	CCWGG	4 cut(s)	288, 1164, 2187, 2466
Alw21I	GWGCWC	2 cut(s)	751, 2119
Alw26I	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
AlwI	GGATC	8 cut(s)	1202, 1632, 1653, 1703, 1716, 1868, 2138, 2349
AlwNI	CAGNNNCTG	3 cut(s)	956, 1844, 2219
Ama87I	CYCGRG	1 cut(s)	84
Aor51HI	AGCGCT	1 cut(s)	2539
ApaI	GGGCCC	1 cut(s)	217
ApeKI	GCWGC	4 cut(s)	1611, 1807, 1961, 2540
ApoI	RAATTY	3 cut(s)	428, 757, 1714
ArsI	GACNNNNNNTTYG	2 cut(s)	1218, 1250
Asp700I	GAANNNNTTC	2 cut(s)	1726, 2196
AspLEI	GCGC	3 cut(s)	1609, 1611, 2540
AspS9I	GGNCC	5 cut(s)	12, 213, 214, 1162, 1761
AsuC2I	CCSGG	3 cut(s)	1142, 1694, 1812
AsuHPI	GGTGA	5 cut(s)	56, 483, 1324, 1708, 2177
AsuNHI	GCTAGC	1 cut(s)	2506
AvaI	CYCGRG	1 cut(s)	84
AvaII	GGWCC	3 cut(s)	12, 1162, 1761
AxyI	CCTNAGG	1 cut(s)	2256
BaeGI	GKGCMC	1 cut(s)	217
BalI	TGGCCA	1 cut(s)	1061
BamHI	GGATCC	1 cut(s)	1708
BanI	GGYRCC	1 cut(s)	1531
BanII	GRGCYC	1 cut(s)	217
BauI	CACGAG	1 cut(s)	1559
BbsI	GAAGAC	2 cut(s)	2370, 2478
Bbv12I	GWGCWC	2 cut(s)	751, 2119
BbvI	GCAGC	4 cut(s)	1623, 1794, 1973, 2527
BccI	CCATC	8 cut(s)	224, 225, 278, 619, 1313, 1462, 1787, 2081
BciT130I	CCWGG	4 cut(s)	290, 1166, 2189, 2468
BciVI	GTATCC	4 cut(s)	848, 906, 1214, 1441
BclI	TGATCA	1 cut(s)	183
BcnI	CCSGG	3 cut(s)	1142, 1694, 1812
BcoDI	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
BfaI	CTAG	6 cut(s)	21, 795, 1376, 1424, 2180, 2507
BfoI	RGCGCY	1 cut(s)	2541
BfuI	GTATCC	4 cut(s)	848, 906, 1214, 1441
BglII	AGATCT	1 cut(s)	496
BisI	GCNGC	7 cut(s)	64, 1357, 1612, 1808, 1962, 2529, 2541
BlpI	GCTNAGC	1 cut(s)	2052
BlsI	GCNGC	7 cut(s)	65, 1358, 1613, 1809, 1963, 2530, 2542
BmcAI	AGTACT	1 cut(s)	2462
Bme1390I	CCNGG	7 cut(s)	290, 1142, 1166, 1694, 1812, 2189, 2468
Bme18I	GGWCC	3 cut(s)	12, 1162, 1761
BmeT110I	CYCGRG	1 cut(s)	84
BmgT120I	GGNCC	5 cut(s)	12, 213, 214, 1162, 1761
BmiI	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
BmrFI	CCNGG	7 cut(s)	290, 1142, 1166, 1694, 1812, 2189, 2468
BmrI	ACTGGG	1 cut(s)	2014
BmsI	GCATC	5 cut(s)	142, 1168, 1436, 1486, 1794
BmtI	GCTAGC	1 cut(s)	2510
BmuI	ACTGGG	1 cut(s)	2014
BpiI	GAAGAC	2 cut(s)	2370, 2478
BpmI	CTGGAG	1 cut(s)	123
Bpu10I	CCTNAGC	2 cut(s)	1684, 2169
Bpu1102I	GCTNAGC	1 cut(s)	2052
BpuEI	CTTGAG	2 cut(s)	156, 887
BpuMI	CCSGG	3 cut(s)	1142, 1694, 1812
BsaI	GGTCTC	2 cut(s)	525, 2096
BsaJI	CCNNGG	5 cut(s)	289, 1062, 1141, 1360, 1484
BsaWI	WCCGGW	1 cut(s)	2407
BsaXI	ACNNNNNCTCC	4 cut(s)	285, 315, 912, 942
Bsc4I	CCNNNNNNNGG	7 cut(s)	59, 500, 625, 1136, 1140, 2153, 2281
Bse1I	ACTGG	3 cut(s)	2009, 2057, 2213
Bse21I	CCTNAGG	1 cut(s)	2256
Bse3DI	GCAATG	4 cut(s)	165, 1876, 2307, 2322
BseBI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
BseDI	CCNNGG	5 cut(s)	289, 1062, 1141, 1360, 1484
BseGI	GGATG	7 cut(s)	161, 186, 259, 1084, 1324, 1451, 2353
BseLI	CCNNNNNNNGG	7 cut(s)	59, 500, 625, 1136, 1140, 2153, 2281
BseMI	GCAATG	4 cut(s)	165, 1876, 2307, 2322
BseMII	CTCAG	8 cut(s)	105, 696, 963, 2043, 2270, 2472, 2480, 2570
BseNI	ACTGG	3 cut(s)	2009, 2057, 2213
BsePI	GCGCGC	1 cut(s)	1607
BseRI	GAGGAG	2 cut(s)	1669, 2282
BseSI	GKGCMC	1 cut(s)	217
BseXI	GCAGC	4 cut(s)	1623, 1794, 1973, 2527
BseYI	CCCAGC	3 cut(s)	249, 1665, 2447
BsgI	GTGCAG	1 cut(s)	2526
Bsh1236I	CGCG	2 cut(s)	66, 1609
BshNI	GGYRCC	1 cut(s)	1531
BsiHKAI	GWGCWC	2 cut(s)	751, 2119
BsiHKCI	CYCGRG	1 cut(s)	84
BsiSI	CCGG	4 cut(s)	1141, 1693, 1811, 2408
BslFI	GGGAC	2 cut(s)	380, 2408
BslI	CCNNNNNNNGG	7 cut(s)	59, 500, 625, 1136, 1140, 2153, 2281
BsmAI	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
BsmBI	CGTCTC	2 cut(s)	219, 2294
BsmFI	GGGAC	2 cut(s)	380, 2408
BsmI	GAATGC	1 cut(s)	828
Bso31I	GGTCTC	2 cut(s)	525, 2096
BsoBI	CYCGRG	1 cut(s)	84
Bsp120I	GGGCCC	1 cut(s)	213
Bsp1286I	GDGCHC	3 cut(s)	217, 751, 2119
Bsp1720I	GCTNAGC	1 cut(s)	2052
Bsp19I	CCATGG	2 cut(s)	1062, 1484
BspACI	CCGC	9 cut(s)	64, 1250, 1289, 1310, 1357, 1464, 2282, 2528, 2546
BspCNI	CTCAG	8 cut(s)	106, 697, 964, 2044, 2269, 2473, 2481, 2569
BspFNI	CGCG	2 cut(s)	66, 1609
BspHI	TCATGA	1 cut(s)	610
BspLI	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
BspOI	GCTAGC	1 cut(s)	2510
BspPI	GGATC	8 cut(s)	1202, 1632, 1653, 1703, 1716, 1868, 2138, 2349
BspT107I	GGYRCC	1 cut(s)	1531
BspTNI	GGTCTC	2 cut(s)	525, 2096
BsrDI	GCAATG	4 cut(s)	165, 1876, 2307, 2322
BsrI	ACTGG	3 cut(s)	2009, 2057, 2213
BssECI	CCNNGG	5 cut(s)	289, 1062, 1141, 1360, 1484
BssHII	GCGCGC	1 cut(s)	1607
BssSI	CACGAG	1 cut(s)	1559
BssT1I	CCWWGG	3 cut(s)	1062, 1360, 1484
Bst2BI	CACGAG	1 cut(s)	1559
Bst2UI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
Bst4CI	ACNGT	7 cut(s)	97, 134, 886, 1003, 1186, 1551, 2434
Bst6I	CTCTTC	3 cut(s)	68, 644, 1184
BstC8I	GCNNGC	6 cut(s)	531, 1421, 1609, 2428, 2508, 2526
BstDSI	CCRYGG	2 cut(s)	1062, 1484
BstENI	CCTNNNNNAGG	1 cut(s)	1134
BstF5I	GGATG	7 cut(s)	161, 186, 259, 1084, 1324, 1451, 2353
BstFNI	CGCG	2 cut(s)	66, 1609
BstH2I	RGCGCY	1 cut(s)	2541
BstHHI	GCGC	3 cut(s)	1609, 1611, 2540
BstMAI	GTCTC	7 cut(s)	219, 525, 947, 1196, 1376, 2096, 2294
BstMWI	GCNNNNNNNGC	7 cut(s)	98, 888, 1130, 1280, 1604, 2062, 2537
BstNI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
BstNSI	RCATGY	4 cut(s)	481, 1423, 1641, 2371
BstSCI	CCNGG	7 cut(s)	288, 1140, 1164, 1692, 1810, 2187, 2466
BstSLI	GKGCMC	1 cut(s)	217
BstUI	CGCG	2 cut(s)	66, 1609
BstV1I	GCAGC	4 cut(s)	1623, 1794, 1973, 2527
BstV2I	GAAGAC	2 cut(s)	2370, 2478
BstX2I	RGATCY	5 cut(s)	496, 1624, 1708, 2143, 2354
BstYI	RGATCY	5 cut(s)	496, 1624, 1708, 2143, 2354
Bsu36I	CCTNAGG	1 cut(s)	2256
BsuI	GTATCC	4 cut(s)	848, 906, 1214, 1441
BtgI	CCRYGG	2 cut(s)	1062, 1484
BtsCI	GGATG	7 cut(s)	161, 186, 259, 1084, 1324, 1451, 2353
BtsIMutI	CAGTG	3 cut(s)	882, 1191, 2430
Cac8I	GCNNGC	6 cut(s)	531, 1421, 1609, 2428, 2508, 2526
CaiI	CAGNNNCTG	3 cut(s)	956, 1844, 2219
CciI	TCATGA	1 cut(s)	610
CfoI	GCGC	3 cut(s)	1609, 1611, 2540
Cfr13I	GGNCC	5 cut(s)	12, 213, 214, 1162, 1761
CseI	GACGC	1 cut(s)	382
Csp6I	GTAC	2 cut(s)	241, 2461
CspCI	CAANNNNNGTGG	2 cut(s)	440, 475
CviQI	GTAC	2 cut(s)	241, 2461
DraI	TTTAAA	1 cut(s)	1036
EaeI	YGGCCR	3 cut(s)	61, 1059, 2529
Eam1104I	CTCTTC	3 cut(s)	68, 644, 1184
EarI	CTCTTC	3 cut(s)	68, 644, 1184
Eco130I	CCWWGG	3 cut(s)	1062, 1360, 1484
Eco147I	AGGCCT	1 cut(s)	2274
Eco24I	GRGCYC	1 cut(s)	217
Eco31I	GGTCTC	2 cut(s)	525, 2096
Eco47I	GGWCC	3 cut(s)	12, 1162, 1761
Eco47III	AGCGCT	1 cut(s)	2539
Eco57I	CTGAAG	3 cut(s)	1238, 1527, 2436
Eco81I	CCTNAGG	1 cut(s)	2256
Eco88I	CYCGRG	1 cut(s)	84
EcoNI	CCTNNNNNAGG	1 cut(s)	1134
EcoO109I	RGGNCCY	1 cut(s)	12
EcoRI	GAATTC	1 cut(s)	757
EcoRII	CCWGG	4 cut(s)	288, 1164, 2187, 2466
EcoT14I	CCWWGG	3 cut(s)	1062, 1360, 1484
EcoT38I	GRGCYC	1 cut(s)	217
ErhI	CCWWGG	3 cut(s)	1062, 1360, 1484
Esp3I	CGTCTC	2 cut(s)	219, 2294
FalI	AAGNNNNNCTT	2 cut(s)	2304, 2336
FaqI	GGGAC	2 cut(s)	380, 2408
FauI	CCCGC	2 cut(s)	1257, 1457
FauNDI	CATATG	1 cut(s)	1650
FbaI	TGATCA	1 cut(s)	183
Fnu4HI	GCNGC	7 cut(s)	64, 1357, 1612, 1808, 1962, 2529, 2541
FokI	GGATG	7 cut(s)	148, 193, 266, 1071, 1331, 1458, 2360
FriOI	GRGCYC	1 cut(s)	217
Fsp4HI	GCNGC	7 cut(s)	64, 1357, 1612, 1808, 1962, 2529, 2541
FspBI	CTAG	6 cut(s)	21, 795, 1376, 1424, 2180, 2507
GlaI	GCGC	3 cut(s)	1608, 1610, 2539
GluI	GCNGC	7 cut(s)	64, 1357, 1612, 1808, 1962, 2529, 2541
GsaI	CCCAGC	3 cut(s)	253, 1669, 2451
GsuI	CTGGAG	1 cut(s)	123
HaeII	RGCGCY	1 cut(s)	2541
HapII	CCGG	4 cut(s)	1141, 1693, 1811, 2408
HgaI	GACGC	1 cut(s)	382
HhaI	GCGC	3 cut(s)	1609, 1611, 2540
Hin6I	GCGC	3 cut(s)	1607, 1609, 2538
HinP1I	GCGC	3 cut(s)	1607, 1609, 2538
HincII	GTYRAC	2 cut(s)	1480, 2266
HindII	GTYRAC	2 cut(s)	1480, 2266
HindIII	AAGCTT	3 cut(s)	531, 1724, 1949
HpaII	CCGG	4 cut(s)	1141, 1693, 1811, 2408
HphI	GGTGA	5 cut(s)	56, 483, 1324, 1708, 2177
Hpy166II	GTNNAC	4 cut(s)	449, 547, 1480, 2266
Hpy8I	GTNNAC	4 cut(s)	449, 547, 1480, 2266
Hpy99I	CGWCG	1 cut(s)	2363
HpyAV	CCTTC	5 cut(s)	708, 749, 1206, 1211, 1653
HpyCH4III	ACNGT	7 cut(s)	97, 134, 886, 1003, 1186, 1551, 2434
HpyCH4IV	ACGT	1 cut(s)	2287
HpyF10VI	GCNNNNNNNGC	7 cut(s)	98, 888, 1130, 1280, 1604, 2062, 2537
HpySE526I	ACGT	1 cut(s)	2287
HspAI	GCGC	3 cut(s)	1607, 1609, 2538
Ksp22I	TGATCA	1 cut(s)	183
LmnI	GCTCC	4 cut(s)	160, 933, 2173, 2521
Lsp1109I	GCAGC	4 cut(s)	1623, 1794, 1973, 2527
LweI	GCATC	5 cut(s)	142, 1168, 1436, 1486, 1794
MaeI	CTAG	6 cut(s)	21, 795, 1376, 1424, 2180, 2507
MaeII	ACGT	1 cut(s)	2287
MaeIII	GTNAC	3 cut(s)	44, 1303, 2496
MfeI	CAATTG	2 cut(s)	1070, 1284
MflI	RGATCY	5 cut(s)	496, 1624, 1708, 2143, 2354
MhlI	GDGCHC	3 cut(s)	217, 751, 2119
MlsI	TGGCCA	1 cut(s)	1061
MluNI	TGGCCA	1 cut(s)	1061
MlyI	GAGTC	3 cut(s)	41, 1485, 2390
MmeI	TCCRAC	2 cut(s)	1828, 2382
Mox20I	TGGCCA	1 cut(s)	1061
MroXI	GAANNNNTTC	2 cut(s)	1726, 2196
MscI	TGGCCA	1 cut(s)	1061
MseI	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
MslI	CAYNNNNRTG	1 cut(s)	1424
Msp20I	TGGCCA	1 cut(s)	1061
MspA1I	CMGCKG	1 cut(s)	1310
MspI	CCGG	4 cut(s)	1141, 1693, 1811, 2408
MspR9I	CCNGG	7 cut(s)	290, 1142, 1166, 1694, 1812, 2189, 2468
MunI	CAATTG	2 cut(s)	1070, 1284
Mva1269I	GAATGC	1 cut(s)	828
MvaI	CCWGG	4 cut(s)	290, 1166, 2189, 2468
MvnI	CGCG	2 cut(s)	66, 1609
MwoI	GCNNNNNNNGC	7 cut(s)	98, 888, 1130, 1280, 1604, 2062, 2537
NciI	CCSGG	3 cut(s)	1142, 1694, 1812
NcoI	CCATGG	2 cut(s)	1062, 1484
NdeI	CATATG	1 cut(s)	1650
NheI	GCTAGC	1 cut(s)	2506
NlaIV	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
NmuCI	GTSAC	1 cut(s)	44
NspI	RCATGY	4 cut(s)	481, 1423, 1641, 2371
PaeI	GCATGC	1 cut(s)	1423
PagI	TCATGA	1 cut(s)	610
PauI	GCGCGC	1 cut(s)	1607
PceI	AGGCCT	1 cut(s)	2274
PciI	ACATGT	1 cut(s)	2367
PctI	GAATGC	1 cut(s)	828
PdmI	GAANNNNTTC	2 cut(s)	1726, 2196
PflMI	CCANNNNNTGG	4 cut(s)	59, 500, 625, 2153
PkrI	GCNGC	7 cut(s)	65, 1358, 1613, 1809, 1963, 2530, 2542
PleI	GAGTC	3 cut(s)	41, 1484, 2390
PpsI	GAGTC	3 cut(s)	41, 1484, 2390
PpuMI	RGGWCCY	1 cut(s)	12
PscI	ACATGT	1 cut(s)	2367
PsiI	TTATAA	1 cut(s)	1755
Psp5II	RGGWCCY	1 cut(s)	12
Psp6I	CCWGG	4 cut(s)	288, 1164, 2187, 2466
PspFI	CCCAGC	3 cut(s)	249, 1665, 2447
PspGI	CCWGG	4 cut(s)	288, 1164, 2187, 2466
PspN4I	GGNNCC	4 cut(s)	215, 1533, 1691, 1710
PspOMI	GGGCCC	1 cut(s)	213
PspPI	GGNCC	5 cut(s)	12, 213, 214, 1162, 1761
PspPPI	RGGWCCY	1 cut(s)	12
PstNI	CAGNNNCTG	3 cut(s)	956, 1844, 2219
PsuI	RGATCY	5 cut(s)	496, 1624, 1708, 2143, 2354
PteI	GCGCGC	1 cut(s)	1607
RsaI	GTAC	2 cut(s)	242, 2462
RsaNI	GTAC	2 cut(s)	241, 2461
RseI	CAYNNNNRTG	1 cut(s)	1424
SaqAI	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
SatI	GCNGC	7 cut(s)	64, 1357, 1612, 1808, 1962, 2529, 2541
Sau96I	GGNCC	5 cut(s)	12, 213, 214, 1162, 1761
ScaI	AGTACT	1 cut(s)	2462
SchI	GAGTC	3 cut(s)	41, 1485, 2390
ScrFI	CCNGG	7 cut(s)	290, 1142, 1166, 1694, 1812, 2189, 2468
SduI	GDGCHC	3 cut(s)	217, 751, 2119
SfaNI	GCATC	5 cut(s)	142, 1168, 1436, 1486, 1794
SinI	GGWCC	3 cut(s)	12, 1162, 1761
SmiMI	CAYNNNNRTG	1 cut(s)	1424
SmlI	CTYRAG	2 cut(s)	171, 866
SmoI	CTYRAG	2 cut(s)	171, 866
SphI	GCATGC	1 cut(s)	1423
SseBI	AGGCCT	1 cut(s)	2274
SsiI	CCGC	9 cut(s)	64, 1250, 1289, 1310, 1357, 1464, 2282, 2528, 2546
SspMI	CTAG	6 cut(s)	21, 795, 1376, 1424, 2180, 2507
StuI	AGGCCT	1 cut(s)	2274
StyD4I	CCNGG	7 cut(s)	288, 1140, 1164, 1692, 1810, 2187, 2466
StyI	CCWWGG	3 cut(s)	1062, 1360, 1484
TaaI	ACNGT	7 cut(s)	97, 134, 886, 1003, 1186, 1551, 2434
TaiI	ACGT	1 cut(s)	2290
TaqI	TCGA	1 cut(s)	1771
TaqII	GACCGA	2 cut(s)	507, 2426
TatI	WGTACW	1 cut(s)	2460
TauI	GCSGC	3 cut(s)	66, 1359, 2531
Tru1I	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
Tru9I	TTAA	6 cut(s)	327, 657, 894, 1035, 1922, 2250
TscAI	CASTG	3 cut(s)	889, 1191, 2437
TseFI	GTSAC	1 cut(s)	44
TseI	GCWGC	4 cut(s)	1611, 1807, 1961, 2540
Tsp45I	GTSAC	1 cut(s)	44
TspDTI	ATGAA	6 cut(s)	461, 599, 825, 1340, 2223, 2318
TspGWI	ACGGA	3 cut(s)	147, 193, 532
TspRI	CASTG	3 cut(s)	889, 1191, 2437
Van91I	CCANNNNNTGG	4 cut(s)	59, 500, 625, 2153
VpaK11BI	GGWCC	3 cut(s)	12, 1162, 1761
XagI	CCTNNNNNAGG	1 cut(s)	1134
XapI	RAATTY	3 cut(s)	428, 757, 1714
XbaI	TCTAGA	1 cut(s)	2179
XceI	RCATGY	4 cut(s)	481, 1423, 1641, 2371
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2034, 2464
XmnI	GAANNNNTTC	2 cut(s)	1726, 2196
XspI	CTAG	6 cut(s)	21, 795, 1376, 1424, 2180, 2507
ZrmI	AGTACT	1 cut(s)	2462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.