MD06G1067800.v1.1

Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015819
Physical Location & Seq
Forward (+)
15255488 .. 15256936
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1067800.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGACACCCATTCCCTTCCCCCTCCTTTCCATACGAGAGATTTCCACCTGCAGCAGCATCCTCAGTTCCTCCACCAGCAACAGAATTCCGAAGACGAGCAAACAGGAAGCAGTGGCCTTAACAAGGGACAGAAGAGAGAGCGGGATATGGACAACAATGACAGCGGTGGCAATGGAGGCGAATTAGGCAAAGAGCTCAATGTCACCATGTCCGGTGGTGACGGATCAGAAATGACGAGAAGGCCCCGAGGGAGACCTGCCGGATCCAAGAACAAGCCTAAGCCCCCCATCATCATCACCCGGGACAGCGCCAATGCGCTCCGCTCCCACGTCATGGAGATTGCCGACGGGTGTGACATAGTGGAGAGTGTCGCCACCTTTGCTCGCAGGCGCCAGAGAGGCGTCTGCATTATGAGCGGGACCGGCACAGTCACGAATGTGACCCTCAGGCAGCCCGCCTCCCCAGGGTCCGTAGTGACGTTGCACGGTCGATTCGAGATCTTATCACTTGCGGGATCATTTCTTCCCCCACCAGCCCCACCTGCCGCGACTGGTTTGACCATATATCTGGCAGGAGGGCAAGGGCAAGTTGTAGGGGGAAGCGTTGTGGGGACGCTAATTGCATCTGGCCCCGTTGTCATCATGGCAGCTTCGTTTAGCAACGCGGCATATGAGCGGCTTCCACTAGAGGAAGACGAGGGTCAACTGCCAATGCAAGGTGGAGGTGGAAGTGTTGGTTCTCCAACCGGGGTTGGTCATCAGAACCAGCAGCAGCAACAACCGCAGCACCAACAACTTTTGGCTGAAGCTGCGAACTCAAATGCGCCTCTTTTTCATGGGTTGCATCCCAATCTTCTGAATTCCATGGAATTGCCAGCTGAGGCAGCGTACTGGGCTACCAGCCGCCCGCCATTCTAA

Protein Analysis

306

Amino Acids

32.28

Weight (kDa)

6.25

Isoelectric Point (pI)

53.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 108 - 220 2.4e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12050 AT4G22810
fragaria_vesca FvH4_5g00910
malus_domestica MD04G1077100.v1.1 MD06G1067800.v1.1
prunus_persica Prupe.5G082200_v2.0.a1
pyrus_communis pycom04g06790 pycom06g06490
rosa_chinensis RchiOBHm_Chr7g0200471
rosa_laevigata RLG00000003723
rosa_multiflora Rmu_sc0000617.1_g000026
rosa_rugosa Rorug07G0060400
rosa_samantha Rh7AG186000 Rh7DG190100
rosa_wichuraiana Rw7G016310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 57, 550
Acc36I ACCTGC 3 cut(s) 57, 265, 550
AccB1I GGYRCC 1 cut(s) 390
AccB7I CCANNNNNTGG 1 cut(s) 334
AccBSI CCGCTC 4 cut(s) 142, 324, 417, 676
AccII CGCG 2 cut(s) 548, 665
AclWI GGATC 4 cut(s) 232, 258, 271, 523
AcsI RAATTY 2 cut(s) 85, 859
AcuI CTGAAG 1 cut(s) 825
AcyI GRCGYC 2 cut(s) 391, 402
AfaI GTAC 1 cut(s) 890
AfiI CCNNNNNNNGG 4 cut(s) 124, 334, 465, 716
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 463
AleI CACNNNNGTG 1 cut(s) 437
AluBI AGCT 4 cut(s) 196, 650, 809, 878
AluI AGCT 4 cut(s) 196, 650, 809, 878
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 247
AlwI GGATC 4 cut(s) 232, 258, 271, 523
Ama87I CYCGRG 2 cut(s) 246, 300
AoxI GGCC 3 cut(s) 115, 242, 628
ApeKI GCWGC 9 cut(s) 52, 55, 451, 647, 769, 772, 784, 809, 884
ApoI RAATTY 2 cut(s) 85, 859
AspLEI GCGC 4 cut(s) 311, 319, 393, 826
AspS9I GGNCC 4 cut(s) 243, 420, 468, 629
AsuC2I CCSGG 3 cut(s) 301, 302, 748
AsuHPI GGTGA 3 cut(s) 196, 230, 289
AvaI CYCGRG 2 cut(s) 246, 300
AvaII GGWCC 2 cut(s) 420, 468
AxyI CCTNAGG 1 cut(s) 446
BamHI GGATCC 1 cut(s) 263
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 99, 699
Bbv12I GWGCWC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 9 cut(s) 64, 67, 463, 659, 781, 784, 796, 796, 896
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 465
BcnI CCSGG 3 cut(s) 301, 302, 748
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 1 cut(s) 686
BfmI CTRYAG 1 cut(s) 50
BfoI RGCGCY 2 cut(s) 312, 394
BfuAI ACCTGC 3 cut(s) 57, 265, 550
BglI GCCNNNNNGGC 1 cut(s) 399
BglII AGATCT 1 cut(s) 498
Bme1390I CCNGG 4 cut(s) 301, 302, 465, 748
Bme18I GGWCC 2 cut(s) 420, 468
BmeT110I CYCGRG 2 cut(s) 246, 300
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 4 cut(s) 243, 420, 468, 629
BmiI GGNNCC 6 cut(s) 245, 265, 392, 421, 469, 631
BmrFI CCNGG 4 cut(s) 301, 302, 465, 748
BmrI ACTGGG 1 cut(s) 901
BmsI GCATC 3 cut(s) 67, 632, 853
BmuI ACTGGG 1 cut(s) 901
BoxI GACNNNNGTC 1 cut(s) 699
BpiI GAAGAC 2 cut(s) 99, 699
Bpu10I CCTNAGC 2 cut(s) 279, 879
BpuMI CCSGG 3 cut(s) 301, 302, 748
BsaHI GRCGYC 2 cut(s) 391, 402
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 6 cut(s) 247, 300, 463, 464, 747, 864
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 334, 465, 716
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 556, 896
Bse21I CCTNAGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 6 cut(s) 247, 300, 463, 464, 747, 864
BseGI GGATG 2 cut(s) 58, 844
BseLI CCNNNNNNNGG 4 cut(s) 124, 334, 465, 716
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 3 cut(s) 77, 460, 870
BseNI ACTGG 2 cut(s) 556, 896
BseXI GCAGC 9 cut(s) 64, 67, 463, 659, 781, 784, 796, 796, 896
Bsh1236I CGCG 2 cut(s) 548, 665
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 3 cut(s) 117, 244, 630
BshNI GGYRCC 1 cut(s) 390
BsiEI CGRYCG 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 2 cut(s) 246, 300
BsiSI CCGG 5 cut(s) 213, 261, 301, 423, 747
BslFI GGGAC 4 cut(s) 141, 317, 433, 625
BslI CCNNNNNNNGG 4 cut(s) 124, 334, 465, 716
BsmAI GTCTC 1 cut(s) 247
BsmFI GGGAC 4 cut(s) 141, 317, 433, 625
BsnI GGCC 3 cut(s) 117, 244, 630
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 2 cut(s) 246, 300
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 4 cut(s) 224, 263, 498, 515
Bsp19I CCATGG 1 cut(s) 864
BspANI GGCC 3 cut(s) 117, 244, 630
BspCNI CTCAG 3 cut(s) 76, 459, 871
BspFNI CGCG 2 cut(s) 548, 665
BspLI GGNNCC 6 cut(s) 245, 265, 392, 421, 469, 631
BspMAI CTGCAG 1 cut(s) 54
BspMI ACCTGC 3 cut(s) 57, 265, 550
BspPI GGATC 4 cut(s) 232, 258, 271, 523
BspT107I GGYRCC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 247
BsrBI CCGCTC 4 cut(s) 142, 324, 417, 676
BsrDI GCAATG 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 2 cut(s) 556, 896
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 6 cut(s) 247, 300, 463, 464, 747, 864
BssMI GATC 4 cut(s) 224, 263, 498, 515
BssNI GRCGYC 2 cut(s) 391, 402
BssT1I CCWWGG 1 cut(s) 864
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 2 cut(s) 430, 488
Bst6I CTCTTC 1 cut(s) 128
BstACI GRCGYC 2 cut(s) 391, 402
BstC8I GCNNGC 5 cut(s) 385, 389, 456, 876, 908
BstDEI CTNAG 4 cut(s) 63, 279, 446, 879
BstDSI CCRYGG 1 cut(s) 864
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 58, 844
BstFNI CGCG 2 cut(s) 548, 665
BstH2I RGCGCY 2 cut(s) 312, 394
BstHHI GCGC 4 cut(s) 311, 319, 393, 826
BstKTI GATC 4 cut(s) 227, 266, 501, 518
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 4 cut(s) 224, 263, 498, 515
BstMCI CGRYCG 1 cut(s) 490
BstNI CCWGG 1 cut(s) 465
BstPAI GACNNNNGTC 1 cut(s) 699
BstSCI CCNGG 4 cut(s) 299, 300, 463, 746
BstSFI CTRYAG 1 cut(s) 50
BstUI CGCG 2 cut(s) 548, 665
BstV1I GCAGC 9 cut(s) 64, 67, 463, 659, 781, 784, 796, 796, 896
BstV2I GAAGAC 2 cut(s) 99, 699
BstX2I RGATCY 2 cut(s) 263, 498
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 2 cut(s) 263, 498
Bsu36I CCTNAGG 1 cut(s) 446
BsuRI GGCC 3 cut(s) 117, 244, 630
BtgI CCRYGG 1 cut(s) 864
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 2 cut(s) 58, 844
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
BveI ACCTGC 3 cut(s) 57, 265, 550
Cac8I GCNNGC 5 cut(s) 385, 389, 456, 876, 908
CfoI GCGC 4 cut(s) 311, 319, 393, 826
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 4 cut(s) 243, 420, 468, 629
Cfr9I CCCGGG 1 cut(s) 300
CseI GACGC 2 cut(s) 391, 622
Csp6I GTAC 1 cut(s) 889
CviAII CATG 5 cut(s) 208, 334, 643, 836, 865
CviQI GTAC 1 cut(s) 889
DdeI CTNAG 4 cut(s) 63, 279, 446, 879
DinI GGCGCC 1 cut(s) 392
DpnI GATC 4 cut(s) 226, 265, 500, 517
DpnII GATC 4 cut(s) 224, 263, 498, 515
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 864
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 247
Eco47I GGWCC 2 cut(s) 420, 468
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 825
Eco81I CCTNAGG 1 cut(s) 446
Eco88I CYCGRG 2 cut(s) 246, 300
EcoICRI GAGCTC 1 cut(s) 196
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 243
EcoRI GAATTC 2 cut(s) 85, 859
EcoRII CCWGG 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 864
EcoT38I GRGCYC 1 cut(s) 198
EgeI GGCGCC 1 cut(s) 392
EheI GGCGCC 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 864
FaeI CATG 5 cut(s) 211, 337, 646, 839, 868
FaqI GGGAC 4 cut(s) 141, 317, 433, 625
FatI CATG 5 cut(s) 207, 333, 642, 835, 864
FauI CCCGC 5 cut(s) 135, 410, 463, 505, 915
FauNDI CATATG 1 cut(s) 670
FokI GGATG 2 cut(s) 45, 831
FriOI GRGCYC 1 cut(s) 198
FspBI CTAG 1 cut(s) 686
GlaI GCGC 4 cut(s) 310, 318, 392, 825
HaeII RGCGCY 2 cut(s) 312, 394
HaeIII GGCC 3 cut(s) 117, 244, 630
HapII CCGG 5 cut(s) 213, 261, 301, 423, 747
HgaI GACGC 2 cut(s) 391, 622
HhaI GCGC 4 cut(s) 311, 319, 393, 826
Hin1I GRCGYC 2 cut(s) 391, 402
Hin1II CATG 5 cut(s) 211, 337, 646, 839, 868
Hin6I GCGC 4 cut(s) 309, 317, 391, 824
HinP1I GCGC 4 cut(s) 309, 317, 391, 824
HincII GTYRAC 1 cut(s) 704
HindII GTYRAC 1 cut(s) 704
HinfI GANTC 1 cut(s) 492
HpaII CCGG 5 cut(s) 213, 261, 301, 423, 747
HphI GGTGA 3 cut(s) 196, 230, 289
Hpy166II GTNNAC 1 cut(s) 704
Hpy188I TCNGA 4 cut(s) 91, 229, 762, 858
Hpy188III TCNNGA 2 cut(s) 433, 496
Hpy8I GTNNAC 1 cut(s) 704
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 26, 234
HpyCH4III ACNGT 2 cut(s) 430, 488
HpyCH4IV ACGT 2 cut(s) 330, 479
HpyCH4V TGCA 6 cut(s) 52, 408, 484, 623, 715, 844
HpyF3I CTNAG 4 cut(s) 63, 279, 446, 879
HpySE526I ACGT 2 cut(s) 330, 479
Hsp92I GRCGYC 2 cut(s) 391, 402
Hsp92II CATG 5 cut(s) 211, 337, 646, 839, 868
HspAI GCGC 4 cut(s) 309, 317, 391, 824
KasI GGCGCC 1 cut(s) 390
Kzo9I GATC 4 cut(s) 224, 263, 498, 515
LmnI GCTCC 2 cut(s) 324, 329
Lsp1109I GCAGC 9 cut(s) 64, 67, 463, 659, 781, 784, 796, 796, 896
LweI GCATC 3 cut(s) 67, 632, 853
MaeI CTAG 1 cut(s) 686
MaeII ACGT 2 cut(s) 330, 479
MaeIII GTNAC 6 cut(s) 202, 218, 353, 430, 439, 475
MalI GATC 4 cut(s) 226, 265, 500, 517
MbiI CCGCTC 4 cut(s) 142, 324, 417, 676
MboI GATC 4 cut(s) 224, 263, 498, 515
MboII GAAGA 5 cut(s) 104, 145, 515, 704, 845
MflI RGATCY 2 cut(s) 263, 498
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 5 cut(s) 85, 182, 618, 859, 869
Mly113I GGCGCC 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 767
MseI TTAA 1 cut(s) 120
MslI CAYNNNNRTG 1 cut(s) 437
MspA1I CMGCKG 2 cut(s) 165, 878
MspI CCGG 5 cut(s) 213, 261, 301, 423, 747
MspR9I CCNGG 4 cut(s) 301, 302, 465, 748
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 2 cut(s) 548, 665
NarI GGCGCC 1 cut(s) 391
NciI CCSGG 3 cut(s) 301, 302, 748
NcoI CCATGG 1 cut(s) 864
NdeI CATATG 1 cut(s) 670
NdeII GATC 4 cut(s) 224, 263, 498, 515
NlaIII CATG 5 cut(s) 211, 337, 646, 839, 868
NlaIV GGNNCC 6 cut(s) 245, 265, 392, 421, 469, 631
NmuCI GTSAC 6 cut(s) 202, 218, 353, 430, 439, 475
OliI CACNNNNGTG 1 cut(s) 437
PaqCI CACCTGC 2 cut(s) 57, 550
PasI CCCWGGG 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PfeI GAWTC 1 cut(s) 492
PflMI CCANNNNNTGG 1 cut(s) 334
PluTI GGCGCC 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 6 cut(s) 245, 265, 392, 421, 469, 631
PspPI GGNCC 4 cut(s) 243, 420, 468, 629
PstI CTGCAG 1 cut(s) 54
PsuI RGATCY 2 cut(s) 263, 498
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 1 cut(s) 890
RsaNI GTAC 1 cut(s) 889
RseI CAYNNNNRTG 1 cut(s) 437
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 120
Sau3AI GATC 4 cut(s) 224, 263, 498, 515
Sau96I GGNCC 4 cut(s) 243, 420, 468, 629
ScrFI CCNGG 4 cut(s) 301, 302, 465, 748
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 3 cut(s) 67, 632, 853
SfcI CTRYAG 1 cut(s) 50
SfoI GGCGCC 1 cut(s) 392
SinI GGWCC 2 cut(s) 420, 468
SmaI CCCGGG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 437
Sse9I AATT 5 cut(s) 85, 182, 618, 859, 869
SspDI GGCGCC 1 cut(s) 390
SspMI CTAG 1 cut(s) 686
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 299, 300, 463, 746
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 2 cut(s) 430, 488
TaiI ACGT 2 cut(s) 333, 482
TaqI TCGA 2 cut(s) 490, 495
TasI AATT 5 cut(s) 85, 182, 618, 859, 869
TauI GCSGC 4 cut(s) 548, 668, 679, 906
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 6 cut(s) 202, 218, 353, 430, 439, 475
TseI GCWGC 9 cut(s) 52, 55, 451, 647, 769, 772, 784, 809, 884
Tsp45I GTSAC 6 cut(s) 202, 218, 353, 430, 439, 475
TspDTI ATGAA 1 cut(s) 824
TspGWI ACGGA 2 cut(s) 237, 460
TspMI CCCGGG 1 cut(s) 300
TspRI CASTG 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 420, 468
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 2 cut(s) 85, 859
XmaI CCCGGG 1 cut(s) 300
XspI CTAG 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.