MD06G1090900.v1.1

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013398
Physical Location & Seq
Reverse (-)
21980228 .. 21983473
3246 bp
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UTR
Exon/CDS
Intron
MD06G1090900.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGGGTCTTTGTTCGGTGACTGGCCGTCGTACAACCCTCACAACTTCAGCCAGCTCCGACCATCCGATCCTTCAAACCCTTCTTTAAAGAAAATGACACCTGCAACCTACTATCCTACTCACAACCGGACTCTTCCGCCACCTGATCAAGTGATAACTAATGAAGCCAAGAATATCCTTTTGAGGCACATGTATCAGCATTCTGAAGAGAAGTTGAGACAAAAGCGGGCAGCGCCAGAAAAACTCTCACCGGAGCCTGTATGCAAGCAACAGAGGTATTCTGTCTCAGATACTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1

Protein Analysis

100

Amino Acids

11.39

Weight (kDa)

9.37

Isoelectric Point (pI)

74.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDA1 PF10172 3 - 70 2e-27 Det1 complexing ubiquitin ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41560 AT5G41560
fragaria_vesca FvH4_5g03180 FvH4_5g03180 FvH4_5g03180 FvH4_5g03180
malus_domestica MD06G1090900.v1.1 MD14G1111700.v1.1
prunus_persica Prupe.5G106400_v2.0.a1
pyrus_communis pycom06g08650 pycom14g10650
rosa_chinensis RchiOBHm_Chr7g0196601
rosa_multiflora Rmu_sc0006080.1_g000005
rosa_roxburghii Rroxscaffold_3G00259170
rosa_rugosa Rorug07G0035200 Rorug07G0035300
rosa_samantha Rh7DG160300
rosa_wichuraiana Rw7G013910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 109
Acc36I ACCTGC 1 cut(s) 109
AciI CCGC 2 cut(s) 137, 226
AclWI GGATC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 23
AcuI CTGAAG 2 cut(s) 31, 225
AfaI GTAC 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 75
AhdI GACNNNNNGTC 1 cut(s) 25
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw26I GTCTC 2 cut(s) 211, 289
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 230
AspLEI GCGC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 29, 240
BbvI GCAGC 1 cut(s) 242
BccI CCATC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 10
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 211, 289
BfoI RGCGCY 1 cut(s) 236
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
BmeRI GACNNNNNGTC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 255
BsaWI WCCGGW 2 cut(s) 126, 250
Bse1I ACTGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 1 cut(s) 25
BsiSI CCGG 2 cut(s) 127, 251
BsmAI GTCTC 2 cut(s) 211, 289
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 25
Bsp143I GATC 2 cut(s) 67, 145
BspACI CCGC 2 cut(s) 137, 226
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 62
BsrI ACTGG 1 cut(s) 26
BssMI GATC 2 cut(s) 67, 145
Bst6I CTCTTC 2 cut(s) 138, 201
BstC8I GCNNGC 3 cut(s) 53, 228, 266
BstDEI CTNAG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 2 cut(s) 70, 148
BstMAI GTCTC 2 cut(s) 211, 289
BstMBI GATC 2 cut(s) 67, 145
BstMWI GCNNNNNNNGC 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 242
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 62
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 53, 228, 266
CaiI CAGNNNCTG 1 cut(s) 293
CfoI GCGC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 31
CviAII CATG 1 cut(s) 190
CviJI RGCY 5 cut(s) 25, 51, 55, 167, 256
CviKI_1 RGCY 5 cut(s) 25, 51, 55, 167, 256
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 286
DpnI GATC 2 cut(s) 69, 147
DpnII GATC 2 cut(s) 67, 145
DraI TTTAAA 1 cut(s) 87
DriI GACNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 138, 201
Eam1105I GACNNNNNGTC 1 cut(s) 25
EarI CTCTTC 2 cut(s) 138, 201
EciI GGCGGA 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 31, 225
FaeI CATG 1 cut(s) 193
FaiI YATR 2 cut(s) 191, 262
FatI CATG 1 cut(s) 189
FauI CCCGC 1 cut(s) 219
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 1 cut(s) 49
Fsp4HI GCNGC 1 cut(s) 231
GlaI GCGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 127, 251
HhaI GCGC 1 cut(s) 235
Hin1II CATG 1 cut(s) 193
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HinfI GANTC 1 cut(s) 130
HpaII CCGG 2 cut(s) 127, 251
HphI GGTGA 2 cut(s) 29, 240
Hpy188I TCNGA 4 cut(s) 59, 67, 205, 289
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 81, 90
HpyCH4V TGCA 2 cut(s) 104, 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 286
Hsp92II CATG 1 cut(s) 193
HspAI GCGC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 2 cut(s) 67, 145
LmnI GCTCC 2 cut(s) 60, 253
LpnPI CCDG 8 cut(s) 7, 65, 114, 140, 156, 249, 264, 270
Lsp1109I GCAGC 1 cut(s) 242
MaeIII GTNAC 1 cut(s) 17
MalI GATC 2 cut(s) 69, 147
MboI GATC 2 cut(s) 67, 145
MboII GAAGA 2 cut(s) 125, 218
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 3 cut(s) 48, 177, 267
MseI TTAA 1 cut(s) 86
MspI CCGG 2 cut(s) 127, 251
Mva1269I GAATGC 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 232
NdeII GATC 2 cut(s) 67, 145
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 193
PaqCI CACCTGC 1 cut(s) 109
PciI ACATGT 1 cut(s) 189
PctI GAATGC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
PscI ACATGT 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 255
PstNI CAGNNNCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 2 cut(s) 67, 145
SchI GAGTC 1 cut(s) 124
SetI ASST 5 cut(s) 57, 103, 110, 145, 278
SsiI CCGC 2 cut(s) 137, 226
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 177
XceI RCATGY 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.