MD14G1111700.v1.1

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013398
Physical Location & Seq
Reverse (-)
17963071 .. 17965152
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1111700.v1.1.491

Sequence Viewer

Length: 234 bp
ATGAATTACCAACATAGATTCATAAAAATGACACCTGCAACCTACCATCCTACCCACGACCGGACTCTTCCGCCACCTGATCAAGTGATAACTAATGAAGCCAAGAACATCCTTTTGAGGCACATGTATCAGCATTCTGAAGAGAAGTTGAGGCAAAAGCGGGCAGCGCCAGAAAAACTTTCACCAGAGCCTGGATGCAAGCAACTTAGGTCTTCTGTTTCAGATAATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1

Protein Analysis

78

Amino Acids

8.98

Weight (kDa)

9.51

Isoelectric Point (pI)

78.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDA1 PF10172 5 - 48 1.4e-16 Det1 complexing ubiquitin ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41560 AT5G41560
fragaria_vesca FvH4_5g03180 FvH4_5g03180 FvH4_5g03180 FvH4_5g03180
malus_domestica MD06G1090900.v1.1 MD14G1111700.v1.1
prunus_persica Prupe.5G106400_v2.0.a1
pyrus_communis pycom06g08650 pycom14g10650
rosa_chinensis RchiOBHm_Chr7g0196601
rosa_multiflora Rmu_sc0006080.1_g000005
rosa_roxburghii Rroxscaffold_3G00259170
rosa_rugosa Rorug07G0035200 Rorug07G0035300
rosa_samantha Rh7DG160300
rosa_wichuraiana Rw7G013910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AccB7I CCANNNNNTGG 1 cut(s) 191
AciI CCGC 2 cut(s) 71, 160
AcuI CTGAAG 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 60, 191
AflIII ACRYGT 1 cut(s) 123
AjnI CCWGG 1 cut(s) 190
AlwNI CAGNNNCTG 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 164
AspLEI GCGC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 176
BccI CCATC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 192
BclI TGATCA 1 cut(s) 79
BfaI CTAG 1 cut(s) 232
BfoI RGCGCY 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 192
BmsI GCATC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 204
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 191
BseBI CCWGG 1 cut(s) 192
BseGI GGATG 3 cut(s) 46, 108, 200
BseLI CCNNNNNNNGG 2 cut(s) 60, 191
BseXI GCAGC 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 61
BsiSI CCGG 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 60, 191
BsmI GAATGC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 2 cut(s) 71, 160
BspMI ACCTGC 1 cut(s) 43
BssMI GATC 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 192
Bst6I CTCTTC 2 cut(s) 72, 135
BstC8I GCNNGC 2 cut(s) 162, 200
BstDEI CTNAG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 46, 108, 200
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 1 cut(s) 82
BstMBI GATC 1 cut(s) 79
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 204
BtsCI GGATG 3 cut(s) 46, 108, 200
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 2 cut(s) 162, 200
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 169
CviAII CATG 1 cut(s) 124
CviJI RGCY 2 cut(s) 101, 190
CviKI_1 RGCY 2 cut(s) 101, 190
DdeI CTNAG 1 cut(s) 206
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
Eam1104I CTCTTC 2 cut(s) 72, 135
EarI CTCTTC 2 cut(s) 72, 135
EciI GGCGGA 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 190
FaeI CATG 1 cut(s) 127
FaiI YATR 3 cut(s) 15, 23, 125
FatI CATG 1 cut(s) 123
FauI CCCGC 1 cut(s) 153
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 3 cut(s) 33, 95, 207
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 1 cut(s) 232
GlaI GCGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 170
HapII CCGG 1 cut(s) 61
HhaI GCGC 1 cut(s) 169
Hin1II CATG 1 cut(s) 127
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 2 cut(s) 18, 64
HpaII CCGG 1 cut(s) 61
HphI GGTGA 1 cut(s) 174
Hpy188I TCNGA 2 cut(s) 139, 223
HpyCH4V TGCA 2 cut(s) 38, 198
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 1 cut(s) 206
Hsp92II CATG 1 cut(s) 127
HspAI GCGC 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 1 cut(s) 79
LpnPI CCDG 7 cut(s) 48, 74, 90, 177, 183, 198, 204
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 1 cut(s) 185
MaeI CTAG 1 cut(s) 232
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 3 cut(s) 59, 152, 204
MluCI AATT 1 cut(s) 4
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 2 cut(s) 111, 144
MslI CAYNNNNRTG 1 cut(s) 26
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 166
NdeII GATC 1 cut(s) 79
NlaIII CATG 1 cut(s) 127
NspI RCATGY 1 cut(s) 127
PaqCI CACCTGC 1 cut(s) 43
PciI ACATGT 1 cut(s) 123
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 18
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PscI ACATGT 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 26
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 79
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 1 cut(s) 192
SetI ASST 4 cut(s) 37, 44, 79, 212
SfaNI GCATC 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 26
Sse9I AATT 1 cut(s) 4
SsiI CCGC 2 cut(s) 71, 160
SspMI CTAG 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 190
TasI AATT 1 cut(s) 4
TfiI GAWTC 1 cut(s) 18
TseI GCWGC 1 cut(s) 164
TspDTI ATGAA 3 cut(s) 10, 17, 111
Van91I CCANNNNNTGG 1 cut(s) 191
XceI RCATGY 1 cut(s) 127
XspI CTAG 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.