Rorug07G0035300

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013398
Physical Location & Seq
Reverse (-)
2491557 .. 2493750
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0035300.1

Sequence Viewer

Length: 1059 bp
ATGGAAGAGCCCCAATCGTCTCAATCCGGGAAGCTTCTGCGATATGCGCTGCGATCTGTGACTAAACCGAAGGAGGAGAAGCCACCTGCCGCAGAATTGTCCAACCCCTCTGCGTCTAAGAGGGGAAGGCCTGCATCAAATGTGAGCAGAAGCGTGAGTGTGCTTGATCTTTCGGGCAAGGACAAGCCTGTCAAGCCACCCAGGAGGCTCTCTGTTCCTAACAAGGCGGCTGGGACTCCAGCTCCGAAACTGGGTGGGAACATCACTCCCATTTCCGAGGCTAGATCAAGGCGGTCTGCCAAAAGCGAAACGCCTGCTTCTGATGCTTCTACTAGGCTGACAAGTCGGAAGAAGTTCAGTATTCTGTCCTCAGAGTCATACTGGCTTTCGCAGATTAAGCTCTCCGAGGCTGCTGGCAAGCACTCAGTTTCACTTGGGTTTTTCAAACTAGCCTTGGAGGCGGGATGTGAGCAACCTCTTCAGCGATTGCGAGATGAGCTAAAAAGCTATGCACTCCGTCATAAACTTGGTGATGCTGACCTTGCAGCCCCACTGAAAGTGTTATTTGAGAGCTACGGTGTTGTAGAAAACGAGCAGTTGCAGGTCTCCGAAACCTGTTCCCACGTACCTGAAGAGGGGACTCGGTCTTCTGATGAAGATGCCAAGAGCTCTTCATCTACAATGGGAACTAGGAAACTGAAACCCAAGTCGTTGAACACTGATGCTGCTCAAGTTTCTCCGGTCAAAAACAAAGCCAAGAAGGAAATGCCTCAGAAGAGCACTCCTGCAACCAGGACTAGGGCATCATCTGTGGCAAAGAAGTCTTCAACTCCAGGACCTGTTTCTGACTCTGGGATGCGCAGGTCAACAGCAAAGAAACCCCACAACCCGATTAAGCAAGAAGCTAAGAATGAAAGGGATAGGAAGAAGCAGGGGAACAAATCTGCTGTTGAACAAGGCTCAGTTAGCGCTACATCTGCAGAGGAAGCACTGAATGAGAACAAAGAAAATGAGGATGCTGCCTCAATGGAGGAGATAAGTTTGACTGAAGTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1

Protein Analysis

352

Amino Acids

37.93

Weight (kDa)

9.75

Isoelectric Point (pI)

64.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41560 AT5G41560
fragaria_vesca FvH4_5g03180 FvH4_5g03180 FvH4_5g03180 FvH4_5g03180
malus_domestica MD06G1090900.v1.1 MD14G1111700.v1.1
prunus_persica Prupe.5G106400_v2.0.a1
pyrus_communis pycom06g08650 pycom14g10650
rosa_chinensis RchiOBHm_Chr7g0196601
rosa_multiflora Rmu_sc0006080.1_g000005
rosa_roxburghii Rroxscaffold_3G00259170
rosa_rugosa Rorug07G0035200 Rorug07G0035300
rosa_samantha Rh7DG160300
rosa_wichuraiana Rw7G013910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
AasI GACNNNNNNGTC 1 cut(s) 188
Acc16I TGCGCA 1 cut(s) 860
Acc36I ACCTGC 3 cut(s) 94, 592, 852
AciI CCGC 4 cut(s) 90, 227, 292, 461
AcuI CTGAAG 2 cut(s) 464, 651
AfaI GTAC 1 cut(s) 627
AfeI AGCGCT 1 cut(s) 970
AfiI CCNNNNNNNGG 3 cut(s) 251, 635, 798
AgsI TTSAA 4 cut(s) 445, 715, 828, 953
AjnI CCWGG 3 cut(s) 200, 791, 832
AluBI AGCT 8 cut(s) 34, 242, 400, 499, 507, 573, 669, 905
AluI AGCT 8 cut(s) 34, 242, 400, 499, 507, 573, 669, 905
Alw21I GWGCWC 2 cut(s) 671, 782
Alw26I GTCTC 2 cut(s) 24, 610
AlwNI CAGNNNCTG 1 cut(s) 839
Aor51HI AGCGCT 1 cut(s) 970
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 5 cut(s) 49, 410, 545, 725, 1019
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 3 cut(s) 49, 861, 971
AspS9I GGNCC 1 cut(s) 836
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 542
AvaII GGWCC 1 cut(s) 836
BanII GRGCYC 2 cut(s) 12, 671
BbsI GAAGAC 2 cut(s) 639, 816
Bbv12I GWGCWC 2 cut(s) 671, 782
BbvI GCAGC 5 cut(s) 36, 397, 557, 712, 1006
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 3 cut(s) 202, 793, 834
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 2 cut(s) 24, 610
BfaI CTAG 5 cut(s) 282, 333, 449, 690, 798
BfmI CTRYAG 1 cut(s) 978
BfoI RGCGCY 1 cut(s) 972
BfuAI ACCTGC 3 cut(s) 94, 592, 852
BglI GCCNNNNNGGC 1 cut(s) 458
BisI GCNGC 7 cut(s) 50, 90, 228, 411, 546, 726, 1020
BlsI GCNGC 7 cut(s) 51, 91, 229, 412, 547, 727, 1021
Bme1390I CCNGG 4 cut(s) 28, 202, 793, 834
Bme18I GGWCC 1 cut(s) 836
BmgT120I GGNCC 1 cut(s) 836
BmrFI CCNGG 4 cut(s) 28, 202, 793, 834
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 8 cut(s) 143, 313, 523, 649, 712, 812, 846, 1006
BmuI ACTGGG 1 cut(s) 260
BpiI GAAGAC 2 cut(s) 639, 816
BpmI CTGGAG 2 cut(s) 222, 816
BpuEI CTTGAG 1 cut(s) 714
BpuMI CCSGG 1 cut(s) 28
BsaAI YACGTR 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 4 cut(s) 200, 276, 405, 453
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 635, 798
Bse1I ACTGG 2 cut(s) 255, 386
BseBI CCWGG 3 cut(s) 202, 793, 834
BseDI CCNNGG 4 cut(s) 200, 276, 405, 453
BseGI GGATG 3 cut(s) 470, 861, 1021
BseLI CCNNNNNNNGG 3 cut(s) 251, 635, 798
BseMII CTCAG 4 cut(s) 384, 438, 785, 975
BseNI ACTGG 2 cut(s) 255, 386
BseRI GAGGAG 2 cut(s) 89, 1046
BseXI GCAGC 5 cut(s) 36, 397, 557, 712, 1006
BseYI CCCAGC 1 cut(s) 230
BshFI GGCC 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 671, 782
BsiSI CCGG 2 cut(s) 27, 740
BslFI GGGAC 2 cut(s) 247, 652
BslI CCNNNNNNNGG 3 cut(s) 251, 635, 798
BsmAI GTCTC 2 cut(s) 24, 610
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 2 cut(s) 247, 652
BsnI GGCC 1 cut(s) 130
Bso31I GGTCTC 1 cut(s) 610
Bsp1286I GDGCHC 3 cut(s) 12, 671, 782
Bsp143I GATC 3 cut(s) 53, 166, 284
BspACI CCGC 4 cut(s) 90, 227, 292, 461
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 4 cut(s) 383, 437, 784, 974
BspMAI CTGCAG 1 cut(s) 982
BspMI ACCTGC 3 cut(s) 94, 592, 852
BspQI GCTCTTC 2 cut(s) 676, 770
BspTNI GGTCTC 1 cut(s) 610
BsrI ACTGG 2 cut(s) 255, 386
BssECI CCNNGG 4 cut(s) 200, 276, 405, 453
BssMI GATC 3 cut(s) 53, 166, 284
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 202, 793, 834
Bst4CI ACNGT 1 cut(s) 578
Bst6I CTCTTC 4 cut(s) 483, 627, 676, 770
BstBAI YACGTR 1 cut(s) 625
BstC8I GCNNGC 4 cut(s) 132, 315, 415, 419
BstDEI CTNAG 6 cut(s) 117, 370, 424, 771, 906, 961
BstF5I GGATG 3 cut(s) 470, 861, 1021
BstH2I RGCGCY 1 cut(s) 972
BstHHI GCGC 3 cut(s) 49, 861, 971
BstKTI GATC 3 cut(s) 56, 169, 287
BstMAI GTCTC 2 cut(s) 24, 610
BstMBI GATC 3 cut(s) 53, 166, 284
BstNI CCWGG 3 cut(s) 202, 793, 834
BstSCI CCNGG 4 cut(s) 26, 200, 791, 832
BstSFI CTRYAG 1 cut(s) 978
BstV1I GCAGC 5 cut(s) 36, 397, 557, 712, 1006
BstV2I GAAGAC 2 cut(s) 639, 816
BsuRI GGCC 1 cut(s) 130
BtsCI GGATG 3 cut(s) 470, 861, 1021
BtsIMutI CAGTG 3 cut(s) 551, 717, 989
BveI ACCTGC 3 cut(s) 94, 592, 852
Cac8I GCNNGC 4 cut(s) 132, 315, 415, 419
CaiI CAGNNNCTG 1 cut(s) 839
CfoI GCGC 3 cut(s) 49, 861, 971
Cfr13I GGNCC 1 cut(s) 836
CseI GACGC 1 cut(s) 102
Csp6I GTAC 1 cut(s) 626
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 6 cut(s) 117, 370, 424, 771, 906, 961
DpnI GATC 3 cut(s) 55, 168, 286
DpnII GATC 3 cut(s) 53, 166, 284
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 4 cut(s) 483, 627, 676, 770
EarI CTCTTC 4 cut(s) 483, 627, 676, 770
Ecl136II GAGCTC 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 12, 671
Eco31I GGTCTC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 836
Eco47III AGCGCT 1 cut(s) 970
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 464, 651
EcoICRI GAGCTC 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 836
EcoRII CCWGG 3 cut(s) 200, 791, 832
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 2 cut(s) 12, 671
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 24
FaiI YATR 5 cut(s) 45, 379, 510, 522, 1057
FaqI GGGAC 2 cut(s) 247, 652
FauI CCCGC 1 cut(s) 454
Fnu4HI GCNGC 7 cut(s) 50, 90, 228, 411, 546, 726, 1020
FokI GGATG 3 cut(s) 477, 868, 1028
FriOI GRGCYC 2 cut(s) 12, 671
Fsp4HI GCNGC 7 cut(s) 50, 90, 228, 411, 546, 726, 1020
FspBI CTAG 5 cut(s) 282, 333, 449, 690, 798
FspI TGCGCA 1 cut(s) 860
GlaI GCGC 3 cut(s) 48, 860, 970
GluI GCNGC 7 cut(s) 50, 90, 228, 411, 546, 726, 1020
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 2 cut(s) 222, 816
HaeII RGCGCY 1 cut(s) 972
HaeIII GGCC 1 cut(s) 130
HapII CCGG 2 cut(s) 27, 740
HgaI GACGC 1 cut(s) 102
HhaI GCGC 3 cut(s) 49, 861, 971
Hin6I GCGC 3 cut(s) 47, 859, 969
HinP1I GCGC 3 cut(s) 47, 859, 969
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 4 cut(s) 235, 374, 640, 848
HpaII CCGG 2 cut(s) 27, 740
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 1 cut(s) 867
Hpy8I GTNNAC 1 cut(s) 867
HpyAV CCTTC 3 cut(s) 64, 120, 754
HpyCH4III ACNGT 1 cut(s) 578
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 7 cut(s) 134, 512, 545, 601, 788, 980, 1055
HpyF3I CTNAG 6 cut(s) 117, 370, 424, 771, 906, 961
HpySE526I ACGT 1 cut(s) 624
HspAI GCGC 3 cut(s) 47, 859, 969
Kzo9I GATC 3 cut(s) 53, 166, 284
LguI GCTCTTC 2 cut(s) 676, 770
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 5 cut(s) 36, 397, 557, 712, 1006
LweI GCATC 8 cut(s) 143, 313, 523, 649, 712, 812, 846, 1006
MaeI CTAG 5 cut(s) 282, 333, 449, 690, 798
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 1 cut(s) 58
MalI GATC 3 cut(s) 55, 168, 286
MboI GATC 3 cut(s) 53, 166, 284
MhlI GDGCHC 3 cut(s) 12, 671, 782
MluCI AATT 1 cut(s) 95
MlyI GAGTC 4 cut(s) 229, 383, 634, 842
MmeI TCCRAC 2 cut(s) 126, 326
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 396, 894
MspI CCGG 2 cut(s) 27, 740
MspR9I CCNGG 4 cut(s) 28, 202, 793, 834
MvaI CCWGG 3 cut(s) 202, 793, 834
NciI CCSGG 1 cut(s) 28
NdeII GATC 3 cut(s) 53, 166, 284
NmuCI GTSAC 1 cut(s) 58
NsbI TGCGCA 1 cut(s) 860
PaqCI CACCTGC 1 cut(s) 94
PceI AGGCCT 1 cut(s) 130
PciSI GCTCTTC 2 cut(s) 676, 770
PdmI GAANNNNTTC 1 cut(s) 353
PflFI GACNNNGTC 1 cut(s) 643
PfoI TCCNGGA 2 cut(s) 26, 832
PkrI GCNGC 7 cut(s) 51, 91, 229, 412, 547, 727, 1021
PleI GAGTC 4 cut(s) 229, 382, 634, 842
PpsI GAGTC 4 cut(s) 229, 382, 634, 842
Ppu21I YACGTR 1 cut(s) 625
PpuMI RGGWCCY 1 cut(s) 836
Psp124BI GAGCTC 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 3 cut(s) 200, 791, 832
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 3 cut(s) 200, 791, 832
PspPI GGNCC 1 cut(s) 836
PspPPI RGGWCCY 1 cut(s) 836
PstI CTGCAG 1 cut(s) 982
PstNI CAGNNNCTG 1 cut(s) 839
PsyI GACNNNGTC 1 cut(s) 643
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
SacI GAGCTC 1 cut(s) 671
SapI GCTCTTC 2 cut(s) 676, 770
SaqAI TTAA 2 cut(s) 396, 894
SatI GCNGC 7 cut(s) 50, 90, 228, 411, 546, 726, 1020
Sau3AI GATC 3 cut(s) 53, 166, 284
Sau96I GGNCC 1 cut(s) 836
SchI GAGTC 4 cut(s) 229, 383, 634, 842
ScrFI CCNGG 4 cut(s) 28, 202, 793, 834
SduI GDGCHC 3 cut(s) 12, 671, 782
SfaNI GCATC 8 cut(s) 143, 313, 523, 649, 712, 812, 846, 1006
SfcI CTRYAG 1 cut(s) 978
SinI GGWCC 1 cut(s) 836
SmlI CTYRAG 1 cut(s) 729
SmoI CTYRAG 1 cut(s) 729
Sse9I AATT 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 130
SsiI CCGC 4 cut(s) 90, 227, 292, 461
SspMI CTAG 5 cut(s) 282, 333, 449, 690, 798
SstI GAGCTC 1 cut(s) 671
StuI AGGCCT 1 cut(s) 130
StyD4I CCNGG 4 cut(s) 26, 200, 791, 832
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 578
TaiI ACGT 1 cut(s) 627
TaqII GACCGA 1 cut(s) 633
TasI AATT 1 cut(s) 95
TauI GCSGC 2 cut(s) 92, 230
Tru1I TTAA 2 cut(s) 396, 894
Tru9I TTAA 2 cut(s) 396, 894
TscAI CASTG 3 cut(s) 558, 724, 996
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 5 cut(s) 49, 410, 545, 725, 1019
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 3 cut(s) 663, 669, 927
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 3 cut(s) 558, 724, 996
Tth111I GACNNNGTC 1 cut(s) 643
VpaK11BI GGWCC 1 cut(s) 836
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 5 cut(s) 282, 333, 449, 690, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.