pycom06g08650

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013398
Physical Location & Seq
Reverse (-)
13868436 .. 13871044
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08650.3

Sequence Viewer

Length: 297 bp
ATGGGGTCTTTGTTCGGTGACTGGCCGTCGTACAACCCTCACAACTTCAGCCAGCTCCGACCATCCGATCCTTCAAACCCTTCTAAAATGACACCTGCAACCTACTATCCTACTCACGACCGGACTCTTCCGCCACCTGATCAAGTGATAACTAATGAAGCCAAGAATATCCTTTTGAGGCACATGTATCAGCATTCTGAAGAGAAGTTGAGACAAAAGAGGGCGGCGCCCGAAAAACTCTCACCGGAGCCTGTATGCAAGCAACGCAGGTATTCTGTCTCAGATACTGCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1

Protein Analysis

99

Amino Acids

11.31

Weight (kDa)

9.17

Isoelectric Point (pI)

77.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41560 AT5G41560
fragaria_vesca FvH4_5g03180 FvH4_5g03180 FvH4_5g03180 FvH4_5g03180
malus_domestica MD06G1090900.v1.1 MD14G1111700.v1.1
prunus_persica Prupe.5G106400_v2.0.a1
pyrus_communis pycom06g08650 pycom14g10650
rosa_chinensis RchiOBHm_Chr7g0196601
rosa_multiflora Rmu_sc0006080.1_g000005
rosa_roxburghii Rroxscaffold_3G00259170
rosa_rugosa Rorug07G0035200 Rorug07G0035300
rosa_samantha Rh7DG160300
rosa_wichuraiana Rw7G013910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 2 cut(s) 103, 258
AccB1I GGYRCC 1 cut(s) 226
AciI CCGC 2 cut(s) 131, 224
AclWI GGATC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 23
AcuI CTGAAG 2 cut(s) 31, 219
AcyI GRCGYC 1 cut(s) 227
AfaI GTAC 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 1 cut(s) 75
AhdI GACNNNNNGTC 1 cut(s) 25
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw26I GTCTC 2 cut(s) 205, 283
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 229
AsuHPI GGTGA 2 cut(s) 29, 234
BanI GGYRCC 1 cut(s) 226
BccI CCATC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 10
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 2 cut(s) 205, 283
BfoI RGCGCY 1 cut(s) 230
BfuAI ACCTGC 2 cut(s) 103, 258
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmeRI GACNNNNNGTC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 228, 249
BsaHI GRCGYC 1 cut(s) 227
BsaWI WCCGGW 2 cut(s) 120, 244
Bse1I ACTGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 26
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 25
BshNI GGYRCC 1 cut(s) 226
BsiEI CGRYCG 1 cut(s) 121
BsiSI CCGG 2 cut(s) 121, 245
BsmAI GTCTC 2 cut(s) 205, 283
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 1 cut(s) 25
Bsp143I GATC 2 cut(s) 67, 139
BspACI CCGC 2 cut(s) 131, 224
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 293
BspLI GGNNCC 2 cut(s) 228, 249
BspMI ACCTGC 2 cut(s) 103, 258
BspPI GGATC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 226
BsrI ACTGG 1 cut(s) 26
BssMI GATC 2 cut(s) 67, 139
BssNI GRCGYC 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 132, 195
BstACI GRCGYC 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 53, 260
BstDEI CTNAG 1 cut(s) 280
BstF5I GGATG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 2 cut(s) 70, 142
BstMAI GTCTC 2 cut(s) 205, 283
BstMBI GATC 2 cut(s) 67, 139
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 187
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 62
BveI ACCTGC 2 cut(s) 103, 258
Cac8I GCNNGC 2 cut(s) 53, 260
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 1 cut(s) 229
Csp6I GTAC 1 cut(s) 31
CviAII CATG 1 cut(s) 184
CviJI RGCY 5 cut(s) 25, 51, 55, 161, 250
CviKI_1 RGCY 5 cut(s) 25, 51, 55, 161, 250
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 280
DinI GGCGCC 1 cut(s) 228
DpnI GATC 2 cut(s) 69, 141
DpnII GATC 2 cut(s) 67, 139
DriI GACNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 132, 195
Eam1105I GACNNNNNGTC 1 cut(s) 25
EarI CTCTTC 2 cut(s) 132, 195
EciI GGCGGA 1 cut(s) 120
Eco57I CTGAAG 2 cut(s) 31, 219
EgeI GGCGCC 1 cut(s) 228
EheI GGCGCC 1 cut(s) 228
FaeI CATG 1 cut(s) 187
FaiI YATR 2 cut(s) 185, 256
FatI CATG 1 cut(s) 183
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 49
Fsp4HI GCNGC 1 cut(s) 225
GlaI GCGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 121, 245
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HinfI GANTC 1 cut(s) 124
HpaII CCGG 2 cut(s) 121, 245
HphI GGTGA 2 cut(s) 29, 234
Hpy188I TCNGA 4 cut(s) 59, 67, 199, 283
Hpy188III TCNNGA 1 cut(s) 116
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 81, 90
HpyCH4V TGCA 2 cut(s) 98, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 264
HpyF3I CTNAG 1 cut(s) 280
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 227
KasI GGCGCC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 67, 139
LmnI GCTCC 2 cut(s) 60, 247
LpnPI CCDG 8 cut(s) 7, 65, 108, 134, 150, 253, 258, 264
MaeIII GTNAC 1 cut(s) 17
MalI GATC 2 cut(s) 69, 141
MboI GATC 2 cut(s) 67, 139
MboII GAAGA 2 cut(s) 119, 212
Mly113I GGCGCC 1 cut(s) 227
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 3 cut(s) 48, 171, 213
MspI CCGG 2 cut(s) 121, 245
Mva1269I GAATGC 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 264
NarI GGCGCC 1 cut(s) 227
NdeII GATC 2 cut(s) 67, 139
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 2 cut(s) 228, 249
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 103
PciI ACATGT 1 cut(s) 183
PctI GAATGC 1 cut(s) 193
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 118
PluTI GGCGCC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 118
PscI ACATGT 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 228, 249
PstNI CAGNNNCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 67, 139
SchI GAGTC 1 cut(s) 118
SetI ASST 5 cut(s) 57, 97, 104, 139, 272
SfoI GGCGCC 1 cut(s) 228
SsiI CCGC 2 cut(s) 131, 224
SspDI GGCGCC 1 cut(s) 226
TauI GCSGC 1 cut(s) 227
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 171
XceI RCATGY 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.