Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0014435

Physical Location & Seq

Genomic Coordinates Forward (+)

25146041 .. 25150557

Gene Structure 4517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1113500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGCCGGAATCACGGTTTCCCAACTGTGACAACAGCTCTCCATTCCCGTTCCTCTCTTCTCTGCTTTTCCTTCTATTAGTCATGACTACAACAACGGTGGCAAAAGCCTCGACCATGACCGGCGCTAAAGCACCCCCTCCCTGCGTTTCCGATTCCGGCCCCGTAAAACCCACCACCCCATTGGTCGCCTTCCTCGAAAAACTCCAAGAAACCGCCCTTGCCACCTTCGGGAAAAAAGATTTCGACCCAAAATACTACGTTGACTTGTCGCTAAAGCTCGAATTGCCGGCGACTCAGAAGGCTTTCGATCAGCTGCCACGGTCGGCAGGTGGGTCCGTGTCGGCCAAAGATTTGAAGGAGTATGTGGATGAGTACTTCGGTGGTGCAGGGGAGGATATGGTGGTTGCAGAGCCGGTGGATTTCGTGCCGGAGCCGGAGGGGTTCCTGCCGAAGGTGAAGCACCCGGAAGTGAGGGCTTGGGCTCTGGAGGTGCATTCCCTTTGGAAGAACTTGAGCAGGAAGGTCTGTGGTGGGGTCCACAAGAAGCCTGAGCTGCACACACTGCTTCCTCTGCCTGAGGAGTTTGTGATTCCTGGTTCAAGGTTCAGGGAGGTTTATTACTGGGATTCTTACTGGGTGATTAGGGGCTTGCTAGCGAGTAAAATGTATGATACAGCAAAAAATATTGTGACCAATCTCCTTTCCTTGATAGAAGAATATGGATATGTCCTAAATGGTGCAAGGACCTATTACGCGAACAGAAGCCAGCCACCACTTCTGAGTGCAATGGTTCACGAAATATACAAGCGGACGGGCAATGTGGAATTGGCACGGAAGGCTCTCCCTTCACTGATGAAAGAACATGAGTTTTGGAATTCAGGTATTCATAAGGTAATTGTTCAGGATTCTGAAGCTCAGAATCATACTTTGAGTCGATACTACGCAATGTGGAACACACCGAGGCCTGAATCTTCAACCATTGACAAGGAATTGGCTTCCAATATCTCGAACCTTTTTGAGAAACAGCAATTTTACCGGGAAGTGGCATCAACTGCCGAGACTGGATGGGATTTCAGTACAAGATGGATGATGAACCATTCGGATTTTACAACGTTGGCTACCACATGGATTTTACCTGTTGATTTGAATGCATTCTTACTCAGGATGGAACATGACATTGCCTTCCTTGCAAAAGTCACAGGAGACTCTAAAATTTCTGAGCGCTTCCTGAAAGCCTCCGAAGCAAGGCATAAGGCCATAAAAGCTGTTTTCTGGGATGCAAAGAAGGGACAATGGCTAGATTACTGGCTCAACAGAAACACATGCAATGAGGAAGATCATACATGGGAAGCCTGTAACCAGAATCAGAATGTATACGCTTCAAATTTTGTCCCTTTGTGGCTTGAGCCGTTTTACTCAGATGCTTCTGTGGTAGAGAAAGTTACAAAAAGTCTTCGAAGCTCAGGACTGATTTGTCCTGCTGGTATTGCAACATCCCTCACAAACTCAACACAACAATGGGATTTTCCAAATGGTTGGGCCCCACTCCAACACATGATAGTTGAAGGTCTGTTAAATTCTGGATTAGAAGAAGCGAAATCAATGGCGGAAGACATTGCTGCGAGGTGGATCAGAACCAACTATGTTGCCTTCCAGAAAACACACACAATGCATGAAAAATATGATGTGCGAGAGTGCGGAGCAACCGGTGGTGGTGGTGAATACATACCCCAGACTGGATTTGGGTGGTCAAATGGAGTTGCATTGGCATTCTTGGAGGAGTTTGGATGGCCTCAAGACAGAAAGATAGATTGTGACCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004555 GO:0005575 GO:0005623 GO:0005886 GO:0005975 GO:0005984 GO:0005991 GO:0005993 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015927 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0044237 GO:0044238 GO:0044248 GO:0044262 GO:0044275 GO:0044464 GO:0046352 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Trehalase

Protein Analysis

608

Amino Acids

68.6

Weight (kDa)

5.76

Isoelectric Point (pI)

44.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trehalase	PF01204	79 - 596	9.1e-175	Trehalase
MGH1-like_GH	PF22422	252 - 565	1.8e-12	Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24040
fragaria_vesca	FvH4_5g11460 FvH4_5g11460 FvH4_5g11460 FvH4_5g11460
malus_domestica	MD06G1113500.v1.1
prunus_persica	Prupe.5G126300_v2.0.a1
pyrus_communis	pycom06g10610
rosa_chinensis	RchiOBHm_Chr7g0186361
rosa_laevigata	RLG00000004848
rosa_multiflora	Rmu_sc0000645.1_g000048
rosa_roxburghii	Rroxscaffold_3G00268180
rosa_rugosa	Rorug06G0473700
rosa_samantha	Rh7AG067600 Rh7CG080400 Rh7DG081000
rosa_wichuraiana	Rw7G006750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	317
AasI	GACNNNNNNGTC	1 cut(s)	1473
Acc36I	ACCTGC	1 cut(s)	317
AccI	GTMKAC	1 cut(s)	1374
AccII	CGCG	1 cut(s)	755
AciI	CCGC	4 cut(s)	213, 808, 1607, 1698
AclI	AACGTT	1 cut(s)	1112
AclWI	GGATC	1 cut(s)	1637
AcoI	YGGCCR	1 cut(s)	342
AcsI	RAATTY	4 cut(s)	874, 1212, 1384, 1576
AcuI	CTGAAG	1 cut(s)	930
AfaI	GTAC	2 cut(s)	374, 1078
AfeI	AGCGCT	1 cut(s)	1223
AfiI	CCNNNNNNNGG	5 cut(s)	228, 451, 1042, 1245, 1736
AgeI	ACCGGT	1 cut(s)	1706
AgsI	TTSAA	6 cut(s)	355, 600, 975, 1147, 1383, 1565
AjnI	CCWGG	1 cut(s)	592
AluBI	AGCT	7 cut(s)	36, 277, 313, 553, 914, 1265, 1461
AluI	AGCT	7 cut(s)	36, 277, 313, 553, 914, 1265, 1461
Alw26I	GTCTC	2 cut(s)	1052, 1197
AlwI	GGATC	1 cut(s)	1637
Aor51HI	AGCGCT	1 cut(s)	1223
AoxI	GGCC	6 cut(s)	157, 342, 962, 1254, 1539, 1790
ApaI	GGGCCC	1 cut(s)	1543
ApeKI	GCWGC	3 cut(s)	313, 553, 1619
ApoI	RAATTY	4 cut(s)	874, 1212, 1384, 1576
AsiGI	ACCGGT	1 cut(s)	1706
Asp700I	GAANNNNTTC	2 cut(s)	302, 1151
AspLEI	GCGC	2 cut(s)	125, 1224
AspS9I	GGNCC	6 cut(s)	158, 333, 535, 744, 1539, 1540
AsuC2I	CCSGG	2 cut(s)	464, 1037
AsuHPI	GGTGA	3 cut(s)	466, 649, 1730
AsuII	TTCGAA	1 cut(s)	1456
AsuNHI	GCTAGC	1 cut(s)	652
AvaII	GGWCC	3 cut(s)	333, 535, 744
AxyI	CCTNAGG	1 cut(s)	576
BaeGI	GKGCMC	1 cut(s)	1543
BanII	GRGCYC	2 cut(s)	484, 1543
BbsI	GAAGAC	2 cut(s)	1445, 1617
BbvI	GCAGC	3 cut(s)	300, 540, 1606
BccI	CCATC	4 cut(s)	1059, 1077, 1159, 1782
BceAI	ACGGC	1 cut(s)	1393
BcgI	CGANNNNNNTGC	2 cut(s)	90, 124
BciT130I	CCWGG	1 cut(s)	594
BcnI	CCSGG	2 cut(s)	464, 1037
BcoDI	GTCTC	2 cut(s)	1052, 1197
BfaI	CTAG	2 cut(s)	653, 1298
BfoI	RGCGCY	2 cut(s)	126, 1225
BfuAI	ACCTGC	1 cut(s)	317
BisI	GCNGC	3 cut(s)	314, 554, 1620
BlsI	GCNGC	3 cut(s)	315, 555, 1621
BmcAI	AGTACT	1 cut(s)	374
Bme1390I	CCNGG	3 cut(s)	464, 594, 1037
Bme18I	GGWCC	3 cut(s)	333, 535, 744
BmgT120I	GGNCC	6 cut(s)	158, 333, 535, 744, 1539, 1540
BmiI	GGNNCC	7 cut(s)	160, 334, 432, 443, 536, 1541, 1542
BmrFI	CCNGG	3 cut(s)	464, 594, 1037
BmrI	ACTGGG	2 cut(s)	631, 643
BmsI	GCATC	3 cut(s)	1055, 1267, 1411
BmtI	GCTAGC	1 cut(s)	656
BmuI	ACTGGG	2 cut(s)	631, 643
BpiI	GAAGAC	2 cut(s)	1445, 1617
BpmI	CTGGAG	1 cut(s)	506
Bpu10I	CCTNAGC	2 cut(s)	549, 1462
Bpu14I	TTCGAA	1 cut(s)	1456
BpuEI	CTTGAG	3 cut(s)	532, 1424, 1779
BpuMI	CCSGG	2 cut(s)	464, 1037
BsaJI	CCNNGG	2 cut(s)	317, 959
BsaWI	WCCGGW	1 cut(s)	1706
Bsc4I	CCNNNNNNNGG	5 cut(s)	228, 451, 1042, 1245, 1736
Bse118I	RCCGGY	4 cut(s)	119, 286, 412, 1706
Bse1I	ACTGG	5 cut(s)	626, 638, 1066, 1310, 1741
Bse21I	CCTNAGG	1 cut(s)	576
Bse3DI	GCAATG	6 cut(s)	792, 823, 951, 1176, 1333, 1614
BseBI	CCWGG	1 cut(s)	594
BseDI	CCNNGG	2 cut(s)	317, 959
BseGI	GGATG	7 cut(s)	373, 1070, 1092, 1170, 1282, 1493, 1793
BseLI	CCNNNNNNNGG	5 cut(s)	228, 451, 1042, 1245, 1736
BseMI	GCAATG	6 cut(s)	792, 823, 951, 1176, 1333, 1614
BseMII	CTCAG	9 cut(s)	308, 540, 567, 770, 929, 1174, 1209, 1431, 1476
BseNI	ACTGG	5 cut(s)	626, 638, 1066, 1310, 1741
BseRI	GAGGAG	2 cut(s)	593, 1793
BseSI	GKGCMC	1 cut(s)	1543
BseXI	GCAGC	3 cut(s)	300, 540, 1606
BsgI	GTGCAG	2 cut(s)	405, 539
Bsh1236I	CGCG	1 cut(s)	755
Bsh1285I	CGRYCG	1 cut(s)	323
BshFI	GGCC	6 cut(s)	159, 344, 964, 1256, 1541, 1792
BshTI	ACCGGT	1 cut(s)	1706
BsiEI	CGRYCG	1 cut(s)	323
BslFI	GGGAC	2 cut(s)	1302, 1376
BslI	CCNNNNNNNGG	5 cut(s)	228, 451, 1042, 1245, 1736
BsmAI	GTCTC	2 cut(s)	1052, 1197
BsmFI	GGGAC	2 cut(s)	1302, 1376
BsmI	GAATGC	4 cut(s)	493, 1151, 1153, 1769
BsnI	GGCC	6 cut(s)	159, 344, 964, 1256, 1541, 1792
Bsp119I	TTCGAA	1 cut(s)	1456
Bsp120I	GGGCCC	1 cut(s)	1539
Bsp1286I	GDGCHC	2 cut(s)	484, 1543
Bsp143I	GATC	3 cut(s)	307, 1336, 1629
BspACI	CCGC	4 cut(s)	213, 808, 1607, 1698
BspANI	GGCC	6 cut(s)	159, 344, 964, 1256, 1541, 1792
BspCNI	CTCAG	9 cut(s)	307, 541, 568, 771, 928, 1173, 1210, 1430, 1475
BspFNI	CGCG	1 cut(s)	755
BspHI	TCATGA	1 cut(s)	81
BspLI	GGNNCC	7 cut(s)	160, 334, 432, 443, 536, 1541, 1542
BspMI	ACCTGC	1 cut(s)	317
BspOI	GCTAGC	1 cut(s)	656
BspPI	GGATC	1 cut(s)	1637
BspT104I	TTCGAA	1 cut(s)	1456
BsrDI	GCAATG	6 cut(s)	792, 823, 951, 1176, 1333, 1614
BsrFI	RCCGGY	4 cut(s)	119, 286, 412, 1706
BsrI	ACTGG	5 cut(s)	626, 638, 1066, 1310, 1741
BssAI	RCCGGY	4 cut(s)	119, 286, 412, 1706
BssECI	CCNNGG	2 cut(s)	317, 959
BssMI	GATC	3 cut(s)	307, 1336, 1629
BssNAI	GTATAC	1 cut(s)	1375
Bst1107I	GTATAC	1 cut(s)	1375
Bst2UI	CCWGG	1 cut(s)	594
Bst4CI	ACNGT	4 cut(s)	15, 26, 97, 321
Bst6I	CTCTTC	1 cut(s)	61
BstAPI	GCANNNNNTGC	2 cut(s)	562, 1052
BstBI	TTCGAA	1 cut(s)	1456
BstC8I	GCNNGC	4 cut(s)	288, 650, 654, 767
BstDEI	CTNAG	9 cut(s)	294, 549, 576, 779, 915, 1160, 1218, 1417, 1462
BstDSI	CCRYGG	1 cut(s)	317
BstENI	CCTNNNNNAGG	1 cut(s)	449
BstF5I	GGATG	7 cut(s)	373, 1070, 1092, 1170, 1282, 1493, 1793
BstFNI	CGCG	1 cut(s)	755
BstH2I	RGCGCY	2 cut(s)	126, 1225
BstHHI	GCGC	2 cut(s)	125, 1224
BstKTI	GATC	3 cut(s)	310, 1339, 1632
BstMAI	GTCTC	2 cut(s)	1052, 1197
BstMBI	GATC	3 cut(s)	307, 1336, 1629
BstMCI	CGRYCG	1 cut(s)	323
BstNI	CCWGG	1 cut(s)	594
BstNSI	RCATGY	1 cut(s)	1326
BstSCI	CCNGG	3 cut(s)	462, 592, 1035
BstSLI	GKGCMC	1 cut(s)	1543
BstUI	CGCG	1 cut(s)	755
BstV1I	GCAGC	3 cut(s)	300, 540, 1606
BstV2I	GAAGAC	2 cut(s)	1445, 1617
BstXI	CCANNNNNNTGG	2 cut(s)	181, 1536
BstZ17I	GTATAC	1 cut(s)	1375
Bsu36I	CCTNAGG	1 cut(s)	576
BsuRI	GGCC	6 cut(s)	159, 344, 964, 1256, 1541, 1792
BtgI	CCRYGG	1 cut(s)	317
BtsCI	GGATG	7 cut(s)	373, 1070, 1092, 1170, 1282, 1493, 1793
BtsI	GCAGTG	1 cut(s)	560
BtsIMutI	CAGTG	2 cut(s)	560, 848
BveI	ACCTGC	1 cut(s)	317
Cac8I	GCNNGC	4 cut(s)	288, 650, 654, 767
CciI	TCATGA	1 cut(s)	81
CfoI	GCGC	2 cut(s)	125, 1224
Cfr10I	RCCGGY	4 cut(s)	119, 286, 412, 1706
Cfr13I	GGNCC	6 cut(s)	158, 333, 535, 744, 1539, 1540
Csp6I	GTAC	2 cut(s)	373, 1077
CspAI	ACCGGT	1 cut(s)	1706
CspCI	CAANNNNNGTGG	2 cut(s)	78, 113
CviAII	CATG	9 cut(s)	82, 115, 863, 1125, 1172, 1323, 1344, 1555, 1673
CviQI	GTAC	2 cut(s)	373, 1077
DdeI	CTNAG	9 cut(s)	294, 549, 576, 779, 915, 1160, 1218, 1417, 1462
DpnI	GATC	3 cut(s)	309, 1338, 1631
DpnII	GATC	3 cut(s)	307, 1336, 1629
DrdI	GACNNNNNNGTC	1 cut(s)	1473
DseDI	GACNNNNNNGTC	1 cut(s)	1473
EaeI	YGGCCR	1 cut(s)	342
Eam1104I	CTCTTC	1 cut(s)	61
EarI	CTCTTC	1 cut(s)	61
EciI	GGCGGA	1 cut(s)	1622
Eco147I	AGGCCT	1 cut(s)	964
Eco24I	GRGCYC	2 cut(s)	484, 1543
Eco47I	GGWCC	3 cut(s)	333, 535, 744
Eco47III	AGCGCT	1 cut(s)	1223
Eco57I	CTGAAG	1 cut(s)	930
Eco81I	CCTNAGG	1 cut(s)	576
EcoNI	CCTNNNNNAGG	1 cut(s)	449
EcoO109I	RGGNCCY	2 cut(s)	744, 1540
EcoRI	GAATTC	1 cut(s)	874
EcoRII	CCWGG	1 cut(s)	592
EcoT22I	ATGCAT	2 cut(s)	1153, 1674
EcoT38I	GRGCYC	2 cut(s)	484, 1543
FaeI	CATG	9 cut(s)	85, 118, 866, 1128, 1175, 1326, 1347, 1558, 1676
FaqI	GGGAC	2 cut(s)	1302, 1376
FatI	CATG	9 cut(s)	81, 114, 862, 1124, 1171, 1322, 1343, 1554, 1672
FblI	GTMKAC	1 cut(s)	1374
Fnu4HI	GCNGC	3 cut(s)	314, 554, 1620
FokI	GGATG	7 cut(s)	380, 1077, 1099, 1177, 1289, 1480, 1800
FriOI	GRGCYC	2 cut(s)	484, 1543
Fsp4HI	GCNGC	3 cut(s)	314, 554, 1620
FspBI	CTAG	2 cut(s)	653, 1298
GlaI	GCGC	2 cut(s)	124, 1223
GluI	GCNGC	3 cut(s)	314, 554, 1620
GsuI	CTGGAG	1 cut(s)	506
HaeII	RGCGCY	2 cut(s)	126, 1225
HaeIII	GGCC	6 cut(s)	159, 344, 964, 1256, 1541, 1792
HhaI	GCGC	2 cut(s)	125, 1224
Hin1II	CATG	9 cut(s)	85, 118, 866, 1128, 1175, 1326, 1347, 1558, 1676
Hin6I	GCGC	2 cut(s)	123, 1222
HinP1I	GCGC	2 cut(s)	123, 1222
HincII	GTYRAC	1 cut(s)	262
HindII	GTYRAC	1 cut(s)	262
HphI	GGTGA	3 cut(s)	466, 649, 1730
Hpy166II	GTNNAC	4 cut(s)	262, 538, 793, 1375
Hpy8I	GTNNAC	4 cut(s)	262, 538, 793, 1375
HpyCH4III	ACNGT	4 cut(s)	15, 26, 97, 321
HpyCH4IV	ACGT	2 cut(s)	258, 1112
HpyF3I	CTNAG	9 cut(s)	294, 549, 576, 779, 915, 1160, 1218, 1417, 1462
HpySE526I	ACGT	2 cut(s)	258, 1112
Hsp92II	CATG	9 cut(s)	85, 118, 866, 1128, 1175, 1326, 1347, 1558, 1676
HspAI	GCGC	2 cut(s)	123, 1222
KroI	GCCGGC	1 cut(s)	286
KroNI	GCCGGC	1 cut(s)	288
Kzo9I	GATC	3 cut(s)	307, 1336, 1629
LmnI	GCTCC	2 cut(s)	430, 1700
Lsp1109I	GCAGC	3 cut(s)	300, 540, 1606
LweI	GCATC	3 cut(s)	1055, 1267, 1411
MaeI	CTAG	2 cut(s)	653, 1298
MaeII	ACGT	2 cut(s)	258, 1112
MaeIII	GTNAC	6 cut(s)	26, 688, 1195, 1355, 1441, 1814
MalI	GATC	3 cut(s)	309, 1338, 1631
MboI	GATC	3 cut(s)	307, 1336, 1629
MboII	GAAGA	8 cut(s)	48, 517, 725, 963, 1346, 1445, 1601, 1622
MhlI	GDGCHC	2 cut(s)	484, 1543
MluCI	AATT	9 cut(s)	281, 824, 874, 894, 989, 1028, 1212, 1384, 1576
MlyI	GAGTC	3 cut(s)	286, 940, 1199
MmeI	TCCRAC	1 cut(s)	1573
Mph1103I	ATGCAT	2 cut(s)	1153, 1674
MroNI	GCCGGC	1 cut(s)	286
MroXI	GAANNNNTTC	2 cut(s)	302, 1151
MseI	TTAA	2 cut(s)	1574, 1822
MslI	CAYNNNNRTG	1 cut(s)	1516
MspA1I	CMGCKG	1 cut(s)	313
MspR9I	CCNGG	3 cut(s)	464, 594, 1037
Mva1269I	GAATGC	4 cut(s)	493, 1151, 1153, 1769
MvaI	CCWGG	1 cut(s)	594
MvnI	CGCG	1 cut(s)	755
NaeI	GCCGGC	1 cut(s)	288
NciI	CCSGG	2 cut(s)	464, 1037
NdeII	GATC	3 cut(s)	307, 1336, 1629
NgoMIV	GCCGGC	1 cut(s)	286
NheI	GCTAGC	1 cut(s)	652
NlaIII	CATG	9 cut(s)	85, 118, 866, 1128, 1175, 1326, 1347, 1558, 1676
NlaIV	GGNNCC	7 cut(s)	160, 334, 432, 443, 536, 1541, 1542
NmeAIII	GCCGAG	1 cut(s)	1081
NmuCI	GTSAC	4 cut(s)	26, 688, 1195, 1814
NsiI	ATGCAT	2 cut(s)	1153, 1674
NspI	RCATGY	1 cut(s)	1326
NspV	TTCGAA	1 cut(s)	1456
PagI	TCATGA	1 cut(s)	81
PaqCI	CACCTGC	1 cut(s)	317
PceI	AGGCCT	1 cut(s)	964
PcsI	WCGNNNNNNNCGW	1 cut(s)	192
PctI	GAATGC	4 cut(s)	493, 1151, 1153, 1769
PdiI	GCCGGC	1 cut(s)	288
PdmI	GAANNNNTTC	2 cut(s)	302, 1151
PfeI	GAWTC	8 cut(s)	8, 152, 589, 626, 905, 919, 968, 1363
PinAI	ACCGGT	1 cut(s)	1706
PkrI	GCNGC	3 cut(s)	315, 555, 1621
PleI	GAGTC	3 cut(s)	286, 939, 1199
PpsI	GAGTC	3 cut(s)	286, 939, 1199
PpuMI	RGGWCCY	1 cut(s)	744
Psp1406I	AACGTT	1 cut(s)	1112
Psp5II	RGGWCCY	1 cut(s)	744
Psp6I	CCWGG	1 cut(s)	592
PspGI	CCWGG	1 cut(s)	592
PspN4I	GGNNCC	7 cut(s)	160, 334, 432, 443, 536, 1541, 1542
PspOMI	GGGCCC	1 cut(s)	1539
PspPI	GGNCC	6 cut(s)	158, 333, 535, 744, 1539, 1540
PspPPI	RGGWCCY	1 cut(s)	744
PvuII	CAGCTG	1 cut(s)	313
RsaI	GTAC	2 cut(s)	374, 1078
RsaNI	GTAC	2 cut(s)	373, 1077
RseI	CAYNNNNRTG	1 cut(s)	1516
SaqAI	TTAA	2 cut(s)	1574, 1822
SatI	GCNGC	3 cut(s)	314, 554, 1620
Sau3AI	GATC	3 cut(s)	307, 1336, 1629
Sau96I	GGNCC	6 cut(s)	158, 333, 535, 744, 1539, 1540
ScaI	AGTACT	1 cut(s)	374
SchI	GAGTC	3 cut(s)	286, 940, 1199
ScrFI	CCNGG	3 cut(s)	464, 594, 1037
SduI	GDGCHC	2 cut(s)	484, 1543
SfaNI	GCATC	3 cut(s)	1055, 1267, 1411
SfuI	TTCGAA	1 cut(s)	1456
SinI	GGWCC	3 cut(s)	333, 535, 744
SmiMI	CAYNNNNRTG	1 cut(s)	1516
SmlI	CTYRAG	3 cut(s)	511, 1403, 1794
SmoI	CTYRAG	3 cut(s)	511, 1403, 1794
Sse9I	AATT	9 cut(s)	281, 824, 874, 894, 989, 1028, 1212, 1384, 1576
SseBI	AGGCCT	1 cut(s)	964
SsiI	CCGC	4 cut(s)	213, 808, 1607, 1698
SspI	AATATT	1 cut(s)	685
SspMI	CTAG	2 cut(s)	653, 1298
StuI	AGGCCT	1 cut(s)	964
StyD4I	CCNGG	3 cut(s)	462, 592, 1035
TaaI	ACNGT	4 cut(s)	15, 26, 97, 321
TaiI	ACGT	2 cut(s)	261, 1115
TaqI	TCGA	8 cut(s)	110, 195, 243, 279, 306, 934, 1007, 1456
TasI	AATT	9 cut(s)	281, 824, 874, 894, 989, 1028, 1212, 1384, 1576
TatI	WGTACW	2 cut(s)	372, 1076
TfiI	GAWTC	8 cut(s)	8, 152, 589, 626, 905, 919, 968, 1363
Tru1I	TTAA	2 cut(s)	1574, 1822
Tru9I	TTAA	2 cut(s)	1574, 1822
TscAI	CASTG	2 cut(s)	567, 855
TseFI	GTSAC	4 cut(s)	26, 688, 1195, 1814
TseI	GCWGC	3 cut(s)	313, 553, 1619
Tsp45I	GTSAC	4 cut(s)	26, 688, 1195, 1814
TspDTI	ATGAA	4 cut(s)	869, 875, 1106, 1689
TspGWI	ACGGA	2 cut(s)	325, 847
TspRI	CASTG	2 cut(s)	567, 855
VpaK11BI	GGWCC	3 cut(s)	333, 535, 744
XagI	CCTNNNNNAGG	1 cut(s)	449
XapI	RAATTY	4 cut(s)	874, 1212, 1384, 1576
XceI	RCATGY	1 cut(s)	1326
XcmI	CCANNNNNNNNNTGG	2 cut(s)	178, 1739
XmiI	GTMKAC	1 cut(s)	1374
XmnI	GAANNNNTTC	2 cut(s)	302, 1151
XspI	CTAG	2 cut(s)	653, 1298
ZrmI	AGTACT	1 cut(s)	374
Zsp2I	ATGCAT	2 cut(s)	1153, 1674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.