Rh7AG067600

Alpha-trehalose glucohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014435
Physical Location & Seq
Reverse (-)
4699605 .. 4703715
4111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG067600.1

Sequence Viewer

Length: 1767 bp
ATGGCGGACTCTCACATCCAATTCTCCTTAGTGTCGTTATTTTTTTCCTCTGTGGTTTTCTCCACCACCATGACTGTCACGAATGCGAGTGCTTCATGCGATTCCGGCCCCGTCAGGCCCACCACCCCATTGGTCACGTTCCTCGAACAAGTAACACAAACGGCGTTGTCCACCTTCGGAAAACCCCACTTCGACCCCAAACTCTACGTCGATTTGGCGCTCAAGTCCGACCTGTCATTCACCCAAGACGCCTTTCATGAGCTTCCCAGGTCCGCAAACGGGTCAACGTCGGTCAATGATCTCAATGAGTTTATACACAACTACTTTGAGGGCGCAGGGGACGATGTGGTGCTCGCTCAGCCGGAGGATTATGTCCCCGAGCCTGAAGGGTTCTTGCCCAAGGTGAAGAACCCGGAAGTGAGGGCCTGGGCTTTGGAGGTTCACTCGCTTTGGAAGAACTTGAGCCGGAAGGTGGCTGGTGGGGTCCACAAGAGGCCGGAGTTTCACACTCTGCTACCTCTGCCTGAGCAGTTCATTATTCCCGGTTCGAGGTTCAGAGAGGTTTATTATTGGGACTCTTATTGGGTTATTAGGGGATTGCTAGCAAGCAAAATGTATGATACAGCAAAAGCAATTGTGACTAATCTCATTTTCCTCATAGAAGAGTATGGTTACGTCCTTAATGGTGCAAGGGCATATTACACTAACAGGAGCCAACCTCCCCTTCTGAGTGCAATGGTTTCCGAAATCTACAAGAGGACAGGAGATTTGGAGTTCGCTAGAAAAGCTCTGCCCGCACTAATCAAAGAACACGAATTCTGGAATTCAGGTATTCATAAGGTAGTTATTCAGGATGACCAAGCTTGTAATCACACTTTAAGTCGATATTATGCAATGTGGAACCAACCCAGGCCTGAATCTTCAACCACTGACAAGGAACTTGCTTCCAAGATCGAGAATGTTTCTGAGAAACAGCATTTTTACCGCGAAGTAGCTTCAACTGCTGAGACTGGATGGGATTTCAGTACAAGATGGATGAGGAACCATTCAGATTTCACAACATTGTCCACCACATCCATTTTACCAGTTGATTTAAATGCCTTCTTACTGGGAATGGAACGTGACATTGCCTTCCTAGCAAAAGTTACAGGAGACAGCAGCAATTCTGATCACTTCCTGAAGGCTTCGGAAGCAAGACACAAGGCCATTAAATCTGTTTTCTGGAATGCAAAGAAGGGGCAATGGCTTGATTACTGGCTCAATGATAGCTCATGCAATTCAGAACCTCAGACATGGGAAGCTCGTAACCAAAATAAGAATGTATTTGCTTCAAACTTCATCCCTCTGTGGATCGAGTCATTTTACTCAGATACTTCTATGGTCCAGAAAGTTACTACAAGTCTTCAAAGTTCAGGCCTTCTTCGCGCTGCTGGAATTGCAACATCACTGACAAATTCAGGACAACAATGGGACTTTCCAAATGGTTGGGCTCCAATCCAACACATGATAGCTGAAGGTCTGGCAAGGTCTGGATCAGAAGAATCAAAGTTAGTGGGAGAAGAAATTGCTGTAAGGTGGATCAGAACCAATTATAAAGCCTACAAGAAAACAGGTGTGATGCATGAAAAATATGATGTGGAAAAGTGTGGAGAATTTGGAGGTGGTGGTGAATATGTACCCCAGACTGGTTTTGGGTGGTCAAATGGACTTGTATTGGCATTCTTGGAGGAGTTTGGATGGCCTCAAGATAGAAAGATAGATTGCTGA

Protein Analysis

588

Amino Acids

66.49

Weight (kDa)

5.79

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalase PF01204 64 - 579 4.6e-179 Trehalase
MGH1-like_GH PF22422 235 - 551 8.8e-14 Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24040
fragaria_vesca FvH4_5g11460 FvH4_5g11460 FvH4_5g11460 FvH4_5g11460
malus_domestica MD06G1113500.v1.1
prunus_persica Prupe.5G126300_v2.0.a1
pyrus_communis pycom06g10610
rosa_chinensis RchiOBHm_Chr7g0186361
rosa_laevigata RLG00000004848
rosa_multiflora Rmu_sc0000645.1_g000048
rosa_roxburghii Rroxscaffold_3G00268180
rosa_rugosa Rorug06G0473700
rosa_samantha Rh7AG067600 Rh7CG080400 Rh7DG081000
rosa_wichuraiana Rw7G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1593
AccII CGCG 2 cut(s) 987, 1425
AciI CCGC 4 cut(s) 5, 273, 795, 985
AclWI GGATC 3 cut(s) 1358, 1540, 1586
AcsI RAATTY 4 cut(s) 815, 823, 1453, 1652
AcuI CTGAAG 3 cut(s) 405, 1199, 1533
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 2 cut(s) 1027, 1677
AfiI CCNNNNNNNGG 4 cut(s) 279, 472, 549, 1685
AgsI TTSAA 4 cut(s) 924, 999, 1332, 1406
AjnI CCWGG 3 cut(s) 266, 425, 908
AjuI GAANNNNNNNTTGG 4 cut(s) 237, 269, 708, 740
AluBI AGCT 7 cut(s) 262, 788, 863, 995, 1269, 1301, 1511
AluI AGCT 7 cut(s) 262, 788, 863, 995, 1269, 1301, 1511
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 2 cut(s) 1001, 1146
AlwI GGATC 3 cut(s) 1358, 1540, 1586
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 8 cut(s) 106, 116, 423, 494, 911, 1203, 1414, 1739
ApeKI GCWGC 2 cut(s) 1158, 1427
ApoI RAATTY 4 cut(s) 815, 823, 1453, 1652
ArsI GACNNNNNNTTYG 4 cut(s) 192, 224, 751, 783
AspLEI GCGC 3 cut(s) 220, 335, 1427
AspS9I GGNCC 6 cut(s) 107, 117, 270, 423, 484, 1381
AsuC2I CCSGG 2 cut(s) 413, 543
AsuHPI GGTGA 3 cut(s) 232, 415, 1679
AsuNHI GCTAGC 1 cut(s) 601
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 270, 484, 1381
BanII GRGCYC 1 cut(s) 1492
BbsI GAAGAC 1 cut(s) 1394
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 1170, 1414
BccI CCATC 3 cut(s) 1008, 1026, 1731
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 3 cut(s) 268, 427, 910
BclI TGATCA 1 cut(s) 1168
BcnI CCSGG 2 cut(s) 413, 543
BcoDI GTCTC 2 cut(s) 1001, 1146
BfaI CTAG 3 cut(s) 602, 780, 1136
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 2 cut(s) 1159, 1428
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 2 cut(s) 1160, 1429
Bme1390I CCNGG 5 cut(s) 268, 413, 427, 543, 910
Bme18I GGWCC 3 cut(s) 270, 484, 1381
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 6 cut(s) 107, 117, 270, 423, 484, 1381
BmiI GGNNCC 6 cut(s) 109, 485, 713, 902, 1043, 1491
BmrFI CCNGG 5 cut(s) 268, 413, 427, 543, 910
BmrI ACTGGG 1 cut(s) 1118
BmsI GCATC 1 cut(s) 1608
BmtI GCTAGC 1 cut(s) 605
BmuI ACTGGG 1 cut(s) 1118
BpiI GAAGAC 1 cut(s) 1394
BplI GAGNNNNNCTC 4 cut(s) 428, 460, 703, 735
Bpu10I CCTNAGC 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 3 cut(s) 206, 481, 1728
BpuMI CCSGG 2 cut(s) 413, 543
BsaHI GRCGYC 1 cut(s) 249
BsaJI CCNNGG 4 cut(s) 266, 399, 426, 908
Bsc4I CCNNNNNNNGG 4 cut(s) 279, 472, 549, 1685
Bse1I ACTGG 5 cut(s) 1015, 1085, 1113, 1259, 1690
Bse3DI GCAATG 4 cut(s) 741, 900, 1125, 1247
BseBI CCWGG 3 cut(s) 268, 427, 910
BseDI CCNNGG 4 cut(s) 266, 399, 426, 908
BseGI GGATG 7 cut(s) 15, 859, 1019, 1041, 1073, 1338, 1742
BseLI CCNNNNNNNGG 4 cut(s) 279, 472, 549, 1685
BseMI GCAATG 4 cut(s) 741, 900, 1125, 1247
BseMII CTCAG 7 cut(s) 371, 516, 719, 957, 996, 1301, 1380
BseNI ACTGG 5 cut(s) 1015, 1085, 1113, 1259, 1690
BseRI GAGGAG 1 cut(s) 1742
BseXI GCAGC 2 cut(s) 1170, 1414
Bsh1236I CGCG 2 cut(s) 987, 1425
BshFI GGCC 8 cut(s) 108, 118, 425, 496, 913, 1205, 1416, 1741
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 6 cut(s) 105, 362, 413, 466, 497, 543
BslFI GGGAC 4 cut(s) 353, 359, 587, 1484
BslI CCNNNNNNNGG 4 cut(s) 279, 472, 549, 1685
BsmAI GTCTC 2 cut(s) 1001, 1146
BsmFI GGGAC 4 cut(s) 353, 359, 587, 1484
BsmI GAATGC 3 cut(s) 88, 1231, 1718
BsnI GGCC 8 cut(s) 108, 118, 425, 496, 913, 1205, 1416, 1741
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 354, 1492
Bsp143I GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
Bsp1720I GCTNAGC 1 cut(s) 357
BspACI CCGC 4 cut(s) 5, 273, 795, 985
BspANI GGCC 8 cut(s) 108, 118, 425, 496, 913, 1205, 1416, 1741
BspCNI CTCAG 7 cut(s) 370, 517, 720, 958, 997, 1300, 1379
BspFNI CGCG 2 cut(s) 987, 1425
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 6 cut(s) 109, 485, 713, 902, 1043, 1491
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 3 cut(s) 1358, 1540, 1586
BsrDI GCAATG 4 cut(s) 741, 900, 1125, 1247
BsrI ACTGG 5 cut(s) 1015, 1085, 1113, 1259, 1690
BssECI CCNNGG 4 cut(s) 266, 399, 426, 908
BssMI GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 3 cut(s) 268, 427, 910
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 249
BstC8I GCNNGC 4 cut(s) 354, 603, 607, 795
BstDEI CTNAG 8 cut(s) 28, 357, 525, 728, 966, 1005, 1287, 1366
BstF5I GGATG 7 cut(s) 15, 859, 1019, 1041, 1073, 1338, 1742
BstFNI CGCG 2 cut(s) 987, 1425
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 3 cut(s) 220, 335, 1427
BstKTI GATC 6 cut(s) 301, 954, 1171, 1353, 1535, 1581
BstMAI GTCTC 2 cut(s) 1001, 1146
BstMBI GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
BstNI CCWGG 3 cut(s) 268, 427, 910
BstSCI CCNGG 5 cut(s) 266, 411, 425, 541, 908
BstUI CGCG 2 cut(s) 987, 1425
BstV1I GCAGC 2 cut(s) 1170, 1414
BstV2I GAAGAC 1 cut(s) 1394
BstXI CCANNNNNNTGG 2 cut(s) 130, 1485
BsuRI GGCC 8 cut(s) 108, 118, 425, 496, 913, 1205, 1416, 1741
BtsCI GGATG 7 cut(s) 15, 859, 1019, 1041, 1073, 1338, 1742
BtsIMutI CAGTG 2 cut(s) 927, 1445
Cac8I GCNNGC 4 cut(s) 354, 603, 607, 795
CciI TCATGA 1 cut(s) 256
CfoI GCGC 3 cut(s) 220, 335, 1427
Cfr13I GGNCC 6 cut(s) 107, 117, 270, 423, 484, 1381
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 1026, 1676
CspCI CAANNNNNGTGG 2 cut(s) 1533, 1568
CviAII CATG 7 cut(s) 70, 96, 257, 1272, 1293, 1504, 1622
CviQI GTAC 2 cut(s) 1026, 1676
DdeI CTNAG 8 cut(s) 28, 357, 525, 728, 966, 1005, 1287, 1366
DpnI GATC 6 cut(s) 300, 953, 1170, 1352, 1534, 1580
DpnII GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
DraI TTTAAA 1 cut(s) 1095
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 399
Eco147I AGGCCT 2 cut(s) 913, 1416
Eco24I GRGCYC 1 cut(s) 1492
Eco47I GGWCC 3 cut(s) 270, 484, 1381
Eco57I CTGAAG 3 cut(s) 405, 1199, 1533
Eco88I CYCGRG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 423
EcoRI GAATTC 2 cut(s) 815, 823
EcoRII CCWGG 3 cut(s) 266, 425, 908
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 1623
EcoT38I GRGCYC 1 cut(s) 1492
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 7 cut(s) 73, 99, 260, 1275, 1296, 1507, 1625
FaqI GGGAC 4 cut(s) 353, 359, 587, 1484
FatI CATG 7 cut(s) 69, 95, 256, 1271, 1292, 1503, 1621
FauI CCCGC 1 cut(s) 802
FbaI TGATCA 1 cut(s) 1168
Fnu4HI GCNGC 2 cut(s) 1159, 1428
FokI GGATG 7 cut(s) 2, 866, 1026, 1048, 1060, 1325, 1749
FriOI GRGCYC 1 cut(s) 1492
Fsp4HI GCNGC 2 cut(s) 1159, 1428
FspBI CTAG 3 cut(s) 602, 780, 1136
GlaI GCGC 3 cut(s) 219, 334, 1426
GluI GCNGC 2 cut(s) 1159, 1428
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 8 cut(s) 108, 118, 425, 496, 913, 1205, 1416, 1741
HapII CCGG 6 cut(s) 105, 362, 413, 466, 497, 543
HgaI GACGC 1 cut(s) 257
HhaI GCGC 3 cut(s) 220, 335, 1427
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 7 cut(s) 73, 99, 260, 1275, 1296, 1507, 1625
Hin6I GCGC 3 cut(s) 218, 333, 1425
HinP1I GCGC 3 cut(s) 218, 333, 1425
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 6 cut(s) 8, 101, 575, 917, 1355, 1541
HpaII CCGG 6 cut(s) 105, 362, 413, 466, 497, 543
HphI GGTGA 3 cut(s) 232, 415, 1679
Hpy166II GTNNAC 5 cut(s) 171, 285, 442, 487, 1068
Hpy8I GTNNAC 5 cut(s) 171, 285, 442, 487, 1068
Hpy99I CGWCG 2 cut(s) 212, 292
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 5 cut(s) 137, 207, 287, 675, 1120
HpyCH4V TGCA 7 cut(s) 689, 734, 893, 1229, 1275, 1439, 1621
HpyF3I CTNAG 8 cut(s) 28, 357, 525, 728, 966, 1005, 1287, 1366
HpySE526I ACGT 5 cut(s) 137, 207, 287, 675, 1120
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 7 cut(s) 73, 99, 260, 1275, 1296, 1507, 1625
HspAI GCGC 3 cut(s) 218, 333, 1425
Ksp22I TGATCA 1 cut(s) 1168
Kzo9I GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
LmnI GCTCC 2 cut(s) 711, 1495
Lsp1109I GCAGC 2 cut(s) 1170, 1414
LweI GCATC 1 cut(s) 1608
MaeI CTAG 3 cut(s) 602, 780, 1136
MaeII ACGT 5 cut(s) 137, 207, 287, 675, 1120
MaeIII GTNAC 9 cut(s) 76, 133, 151, 637, 671, 1121, 1144, 1304, 1390
MalI GATC 6 cut(s) 300, 953, 1170, 1352, 1534, 1580
MboI GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
MboII GAAGA 8 cut(s) 418, 466, 674, 912, 1394, 1412, 1550, 1571
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 2 cut(s) 354, 1492
MlyI GAGTC 3 cut(s) 2, 569, 1364
MmeI TCCRAC 2 cut(s) 252, 1522
Mph1103I ATGCAT 1 cut(s) 1623
MseI TTAA 4 cut(s) 681, 878, 1094, 1209
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 6 cut(s) 105, 362, 413, 466, 497, 543
MspR9I CCNGG 5 cut(s) 268, 413, 427, 543, 910
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 3 cut(s) 88, 1231, 1718
MvaI CCWGG 3 cut(s) 268, 427, 910
MvnI CGCG 2 cut(s) 987, 1425
NciI CCSGG 2 cut(s) 413, 543
NdeII GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 7 cut(s) 73, 99, 260, 1275, 1296, 1507, 1625
NlaIV GGNNCC 6 cut(s) 109, 485, 713, 902, 1043, 1491
NmuCI GTSAC 4 cut(s) 76, 133, 637, 1121
NsiI ATGCAT 1 cut(s) 1623
PagI TCATGA 1 cut(s) 256
PceI AGGCCT 2 cut(s) 913, 1416
PctI GAATGC 3 cut(s) 88, 1231, 1718
PfeI GAWTC 3 cut(s) 101, 917, 1541
PkrI GCNGC 2 cut(s) 1160, 1429
PleI GAGTC 3 cut(s) 2, 569, 1363
PpsI GAGTC 3 cut(s) 2, 569, 1363
PsiI TTATAA 1 cut(s) 1593
Psp6I CCWGG 3 cut(s) 266, 425, 908
PspGI CCWGG 3 cut(s) 266, 425, 908
PspN4I GGNNCC 6 cut(s) 109, 485, 713, 902, 1043, 1491
PspPI GGNCC 6 cut(s) 107, 117, 270, 423, 484, 1381
RsaI GTAC 2 cut(s) 1027, 1677
RsaNI GTAC 2 cut(s) 1026, 1676
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 4 cut(s) 681, 878, 1094, 1209
SatI GCNGC 2 cut(s) 1159, 1428
Sau3AI GATC 6 cut(s) 298, 951, 1168, 1350, 1532, 1578
Sau96I GGNCC 6 cut(s) 107, 117, 270, 423, 484, 1381
SchI GAGTC 3 cut(s) 2, 569, 1364
ScrFI CCNGG 5 cut(s) 268, 413, 427, 543, 910
SduI GDGCHC 2 cut(s) 354, 1492
SfaNI GCATC 1 cut(s) 1608
SinI GGWCC 3 cut(s) 270, 484, 1381
SmiI ATTTAAAT 1 cut(s) 1095
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 3 cut(s) 221, 460, 1743
SmoI CTYRAG 3 cut(s) 221, 460, 1743
SseBI AGGCCT 2 cut(s) 913, 1416
SsiI CCGC 4 cut(s) 5, 273, 795, 985
SspMI CTAG 3 cut(s) 602, 780, 1136
StuI AGGCCT 2 cut(s) 913, 1416
StyD4I CCNGG 5 cut(s) 266, 411, 425, 541, 908
StyI CCWWGG 1 cut(s) 399
SwaI ATTTAAAT 1 cut(s) 1095
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 5 cut(s) 140, 210, 290, 678, 1123
TaqI TCGA 7 cut(s) 144, 192, 210, 548, 883, 954, 1353
TaqII GACCGA 1 cut(s) 280
TatI WGTACW 1 cut(s) 1025
TfiI GAWTC 3 cut(s) 101, 917, 1541
Tru1I TTAA 4 cut(s) 681, 878, 1094, 1209
Tru9I TTAA 4 cut(s) 681, 878, 1094, 1209
TscAI CASTG 2 cut(s) 934, 1452
TseFI GTSAC 4 cut(s) 76, 133, 637, 1121
TseI GCWGC 2 cut(s) 1158, 1427
Tsp45I GTSAC 4 cut(s) 76, 133, 637, 1121
TspDTI ATGAA 6 cut(s) 84, 245, 523, 824, 1327, 1638
TspRI CASTG 2 cut(s) 934, 1452
VpaK11BI GGWCC 3 cut(s) 270, 484, 1381
XapI RAATTY 4 cut(s) 815, 823, 1453, 1652
XcmI CCANNNNNNNNNTGG 2 cut(s) 127, 1688
XspI CTAG 3 cut(s) 602, 780, 1136
Zsp2I ATGCAT 1 cut(s) 1623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.