Rw7G006750

Alpha-trehalose glucohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014435
Physical Location & Seq
Forward (+)
5764709 .. 5768152
3444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006750.1

Sequence Viewer

Length: 1722 bp
ATGACTGTCACGAATGCGAGTGCTTCATGCGATTCCGGCCCCGTCAGGCCCACCACCCCATTGGTCACGTTCCTCGAACAAGTAACACAAACGGCGTTGTCCACCTTCGGAAAACCCCACTTCGACCCCAAACTCTACGTCGATTTGGCGCTCAAGTCCGACCTGTCATTCACCCAAGACGCCTTTCATGAGCTTCCCAGGTCCGCAAACGGGTCAACGTCGGTCAATGATCTCAATGAGTTTATACACAACTACTTTGAGGGCGCAGGGGACGATGTGGTGCTTGCTCAGCCGGAGGATTATGTCCCCGAGCCTGAAGGGTTCTTGCCCAAGGTGAAGAACCCGGAAGTGAGGGCCTGGGCTTTGGAGGTTCACTCGCTTTGGAAGAACTTGAGCCGGAAGGTGGCTGGTGGGGTCCACAAGACGCCGGAGTTTCACACTCTGCTACCTCTGCCTGAGCAGTTCATTATTCCCGGTTCGAGGTTCAGAGAGGTTTATTATTGGGACTCTTATTGGGTTATTAGGGGATTGCTAGCAAGCAAAATGTATGATACAGCAAAAGCAATTGTGACTAATCTCATTTTCCTCATAGAAGAGTATGGTTACGTCCTTAATGGTGCAAGGGCATATTACACTAACAGGAGCCAACCTCCCCTTCTGAGTGCAATGGTTTCCGAAATCTACAAGAGGACAGGAGATTTGGAGTTCGCTAGAAAAGCTCTGCCCGCACTAATCAAAGAACACGAATTCTGGAATTCAGGTATTCATAAGGTAGTTATTCAGGATGACCAAGCTTGTAATCACACTTTAAGTCGATATTATGCAATGTGGAACCAACCCAGGCCTGAATCTTCAACCACTGACAAGGAACTTGCTTCCAAGATCGAGAATGTTTCTGAGAAACAGCATTTTTACCGCGAAGTAGCTTCAACTGCTGAGACTGGATGGGATTTCAGTACAAGATGGATGAGGAACCATTCAGATTTCACAACATTGTCCACCACATCCATTTTACCAGTTGATTTAAATGCCTTCTTACTGGGAATGGAACGTGACATTGCCTTCCTAGCAAAAGTTACAGGAGACAGCAGCAATTCTGATCACTTCCTGAAGGCTTCGGAAGCAAGACACAAGGCCATTAAATCTGTTTTCTGGAATGCAAAGAAGGGGCAATGGCTTGATTACTGGCTCAATGATAGCTCATGCAATTCAGAACCTCAGACATGGGAAGCTCGTAACCAAAATAAGAATGTATTTGCTTCAAACTTCATCCCTCTGTGGACCGAGTCATTTTACTCAGCTCTTGCTCTATGTGTGAGTGAAGATACTTCTATGGTCCAGAAAGTTACTACAAGTCTTCAAAGTTCAGGCCTTCTTCGCGCTGCTGGAATTGCAACATCACTGACAAATTCAGGACAACAATGGGACTTTCCAAATGGTTGGGCTCCAATCCAACACATGATAGCTGAAGGTCTGGCAAGGTCTGGATCAGAAGAATCAAAGTTAGTGGGAGAAGAAATTGCTGTAAGGTGGATCAGAACCAATTATAAAGCCTACAAGAAAACAGGTGTGATGCATGAAAAATATGATGTGGAAAAGTGTGGAGAATTTGGAGGTGGTGGTGAATATGTACCCCAGACTGGTTTTGGGTGGTCAAATGGACTTGTATTGGCATTCTTGGAGGAGTTTGGATGGCCTCAAGATAGAAAGATAGATTGCTGA

Protein Analysis

573

Amino Acids

64.67

Weight (kDa)

5.67

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalase PF01204 41 - 564 2.8e-177 Trehalase
MGH1-like_GH PF22422 212 - 536 2e-13 Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24040
fragaria_vesca FvH4_5g11460 FvH4_5g11460 FvH4_5g11460 FvH4_5g11460
malus_domestica MD06G1113500.v1.1
prunus_persica Prupe.5G126300_v2.0.a1
pyrus_communis pycom06g10610
rosa_chinensis RchiOBHm_Chr7g0186361
rosa_laevigata RLG00000004848
rosa_multiflora Rmu_sc0000645.1_g000048
rosa_roxburghii Rroxscaffold_3G00268180
rosa_rugosa Rorug06G0473700
rosa_samantha Rh7AG067600 Rh7CG080400 Rh7DG081000
rosa_wichuraiana Rw7G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1548
AccII CGCG 2 cut(s) 918, 1380
AciI CCGC 3 cut(s) 204, 726, 916
AclWI GGATC 2 cut(s) 1495, 1541
AcsI RAATTY 4 cut(s) 746, 754, 1408, 1607
AcuI CTGAAG 3 cut(s) 336, 1130, 1488
AcyI GRCGYC 2 cut(s) 180, 425
AfaI GTAC 2 cut(s) 958, 1632
AfiI CCNNNNNNNGG 4 cut(s) 210, 403, 480, 1640
AgsI TTSAA 4 cut(s) 855, 930, 1263, 1361
AjnI CCWGG 3 cut(s) 197, 356, 839
AjuI GAANNNNNNNTTGG 4 cut(s) 168, 200, 639, 671
AluBI AGCT 8 cut(s) 193, 719, 794, 926, 1200, 1232, 1301, 1466
AluI AGCT 8 cut(s) 193, 719, 794, 926, 1200, 1232, 1301, 1466
Alw26I GTCTC 2 cut(s) 932, 1077
AlwI GGATC 2 cut(s) 1495, 1541
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 7 cut(s) 37, 47, 354, 842, 1134, 1369, 1694
ApeKI GCWGC 2 cut(s) 1089, 1382
ApoI RAATTY 4 cut(s) 746, 754, 1408, 1607
ArsI GACNNNNNNTTYG 4 cut(s) 123, 155, 682, 714
AspLEI GCGC 3 cut(s) 151, 266, 1382
AspS9I GGNCC 7 cut(s) 38, 48, 201, 354, 415, 1281, 1336
AsuC2I CCSGG 2 cut(s) 344, 474
AsuHPI GGTGA 3 cut(s) 163, 346, 1634
AsuNHI GCTAGC 1 cut(s) 532
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 4 cut(s) 201, 415, 1281, 1336
BanII GRGCYC 1 cut(s) 1447
BbsI GAAGAC 1 cut(s) 1349
BbvI GCAGC 2 cut(s) 1101, 1369
BccI CCATC 3 cut(s) 939, 957, 1686
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 3 cut(s) 199, 358, 841
BclI TGATCA 1 cut(s) 1099
BcnI CCSGG 2 cut(s) 344, 474
BcoDI GTCTC 2 cut(s) 932, 1077
BfaI CTAG 3 cut(s) 533, 711, 1067
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 2 cut(s) 1090, 1383
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 2 cut(s) 1091, 1384
Bme1390I CCNGG 5 cut(s) 199, 344, 358, 474, 841
Bme18I GGWCC 4 cut(s) 201, 415, 1281, 1336
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 7 cut(s) 38, 48, 201, 354, 415, 1281, 1336
BmiI GGNNCC 6 cut(s) 40, 416, 644, 833, 974, 1446
BmrFI CCNGG 5 cut(s) 199, 344, 358, 474, 841
BmrI ACTGGG 1 cut(s) 1049
BmsI GCATC 1 cut(s) 1563
BmtI GCTAGC 1 cut(s) 536
BmuI ACTGGG 1 cut(s) 1049
BpiI GAAGAC 1 cut(s) 1349
BplI GAGNNNNNCTC 4 cut(s) 359, 391, 634, 666
Bpu10I CCTNAGC 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 3 cut(s) 137, 412, 1683
BpuMI CCSGG 2 cut(s) 344, 474
BsaHI GRCGYC 2 cut(s) 180, 425
BsaJI CCNNGG 4 cut(s) 197, 330, 357, 839
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 403, 480, 1640
Bse1I ACTGG 5 cut(s) 946, 1016, 1044, 1190, 1645
Bse3DI GCAATG 4 cut(s) 672, 831, 1056, 1178
BseBI CCWGG 3 cut(s) 199, 358, 841
BseDI CCNNGG 4 cut(s) 197, 330, 357, 839
BseGI GGATG 6 cut(s) 790, 950, 972, 1004, 1269, 1697
BseLI CCNNNNNNNGG 4 cut(s) 210, 403, 480, 1640
BseMI GCAATG 4 cut(s) 672, 831, 1056, 1178
BseMII CTCAG 7 cut(s) 302, 447, 650, 888, 927, 1232, 1311
BseNI ACTGG 5 cut(s) 946, 1016, 1044, 1190, 1645
BseRI GAGGAG 1 cut(s) 1697
BseXI GCAGC 2 cut(s) 1101, 1369
Bsh1236I CGCG 2 cut(s) 918, 1380
BshFI GGCC 7 cut(s) 39, 49, 356, 844, 1136, 1371, 1696
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 6 cut(s) 36, 293, 344, 397, 428, 474
BslFI GGGAC 4 cut(s) 284, 290, 518, 1439
BslI CCNNNNNNNGG 4 cut(s) 210, 403, 480, 1640
BsmAI GTCTC 2 cut(s) 932, 1077
BsmFI GGGAC 4 cut(s) 284, 290, 518, 1439
BsmI GAATGC 3 cut(s) 19, 1162, 1673
BsnI GGCC 7 cut(s) 39, 49, 356, 844, 1136, 1371, 1696
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 1447
Bsp143I GATC 5 cut(s) 229, 882, 1099, 1487, 1533
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 3 cut(s) 204, 726, 916
BspANI GGCC 7 cut(s) 39, 49, 356, 844, 1136, 1371, 1696
BspCNI CTCAG 7 cut(s) 301, 448, 651, 889, 928, 1231, 1310
BspFNI CGCG 2 cut(s) 918, 1380
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 6 cut(s) 40, 416, 644, 833, 974, 1446
BspOI GCTAGC 1 cut(s) 536
BspPI GGATC 2 cut(s) 1495, 1541
BsrDI GCAATG 4 cut(s) 672, 831, 1056, 1178
BsrI ACTGG 5 cut(s) 946, 1016, 1044, 1190, 1645
BssECI CCNNGG 4 cut(s) 197, 330, 357, 839
BssMI GATC 5 cut(s) 229, 882, 1099, 1487, 1533
BssNI GRCGYC 2 cut(s) 180, 425
BssT1I CCWWGG 1 cut(s) 330
Bst2UI CCWGG 3 cut(s) 199, 358, 841
Bst4CI ACNGT 1 cut(s) 7
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 2 cut(s) 180, 425
BstC8I GCNNGC 4 cut(s) 285, 534, 538, 726
BstDEI CTNAG 7 cut(s) 288, 456, 659, 897, 936, 1218, 1297
BstF5I GGATG 6 cut(s) 790, 950, 972, 1004, 1269, 1697
BstFNI CGCG 2 cut(s) 918, 1380
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 3 cut(s) 151, 266, 1382
BstKTI GATC 5 cut(s) 232, 885, 1102, 1490, 1536
BstMAI GTCTC 2 cut(s) 932, 1077
BstMBI GATC 5 cut(s) 229, 882, 1099, 1487, 1533
BstNI CCWGG 3 cut(s) 199, 358, 841
BstSCI CCNGG 5 cut(s) 197, 342, 356, 472, 839
BstUI CGCG 2 cut(s) 918, 1380
BstV1I GCAGC 2 cut(s) 1101, 1369
BstV2I GAAGAC 1 cut(s) 1349
BstXI CCANNNNNNTGG 2 cut(s) 61, 1440
BsuRI GGCC 7 cut(s) 39, 49, 356, 844, 1136, 1371, 1696
BtsCI GGATG 6 cut(s) 790, 950, 972, 1004, 1269, 1697
BtsIMutI CAGTG 2 cut(s) 858, 1400
Cac8I GCNNGC 4 cut(s) 285, 534, 538, 726
CciI TCATGA 1 cut(s) 187
CfoI GCGC 3 cut(s) 151, 266, 1382
Cfr13I GGNCC 7 cut(s) 38, 48, 201, 354, 415, 1281, 1336
CseI GACGC 2 cut(s) 188, 433
Csp6I GTAC 2 cut(s) 957, 1631
CspCI CAANNNNNGTGG 2 cut(s) 1488, 1523
CviAII CATG 6 cut(s) 27, 188, 1203, 1224, 1459, 1577
CviQI GTAC 2 cut(s) 957, 1631
DdeI CTNAG 7 cut(s) 288, 456, 659, 897, 936, 1218, 1297
DpnI GATC 5 cut(s) 231, 884, 1101, 1489, 1535
DpnII GATC 5 cut(s) 229, 882, 1099, 1487, 1533
DraI TTTAAA 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 330
Eco147I AGGCCT 2 cut(s) 844, 1371
Eco24I GRGCYC 1 cut(s) 1447
Eco47I GGWCC 4 cut(s) 201, 415, 1281, 1336
Eco57I CTGAAG 3 cut(s) 336, 1130, 1488
Eco88I CYCGRG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 354
EcoRI GAATTC 2 cut(s) 746, 754
EcoRII CCWGG 3 cut(s) 197, 356, 839
EcoT14I CCWWGG 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 1578
EcoT38I GRGCYC 1 cut(s) 1447
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 6 cut(s) 30, 191, 1206, 1227, 1462, 1580
FaqI GGGAC 4 cut(s) 284, 290, 518, 1439
FatI CATG 6 cut(s) 26, 187, 1202, 1223, 1458, 1576
FauI CCCGC 1 cut(s) 733
FbaI TGATCA 1 cut(s) 1099
Fnu4HI GCNGC 2 cut(s) 1090, 1383
FokI GGATG 6 cut(s) 797, 957, 979, 991, 1256, 1704
FriOI GRGCYC 1 cut(s) 1447
Fsp4HI GCNGC 2 cut(s) 1090, 1383
FspBI CTAG 3 cut(s) 533, 711, 1067
GlaI GCGC 3 cut(s) 150, 265, 1381
GluI GCNGC 2 cut(s) 1090, 1383
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 7 cut(s) 39, 49, 356, 844, 1136, 1371, 1696
HapII CCGG 6 cut(s) 36, 293, 344, 397, 428, 474
HgaI GACGC 2 cut(s) 188, 433
HhaI GCGC 3 cut(s) 151, 266, 1382
Hin1I GRCGYC 2 cut(s) 180, 425
Hin1II CATG 6 cut(s) 30, 191, 1206, 1227, 1462, 1580
Hin6I GCGC 3 cut(s) 149, 264, 1380
HinP1I GCGC 3 cut(s) 149, 264, 1380
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 792
HinfI GANTC 5 cut(s) 32, 506, 848, 1286, 1496
HpaII CCGG 6 cut(s) 36, 293, 344, 397, 428, 474
HphI GGTGA 3 cut(s) 163, 346, 1634
Hpy166II GTNNAC 6 cut(s) 102, 216, 373, 418, 999, 1281
Hpy8I GTNNAC 6 cut(s) 102, 216, 373, 418, 999, 1281
Hpy99I CGWCG 2 cut(s) 143, 223
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 5 cut(s) 68, 138, 218, 606, 1051
HpyCH4V TGCA 7 cut(s) 620, 665, 824, 1160, 1206, 1394, 1576
HpyF3I CTNAG 7 cut(s) 288, 456, 659, 897, 936, 1218, 1297
HpySE526I ACGT 5 cut(s) 68, 138, 218, 606, 1051
Hsp92I GRCGYC 2 cut(s) 180, 425
Hsp92II CATG 6 cut(s) 30, 191, 1206, 1227, 1462, 1580
HspAI GCGC 3 cut(s) 149, 264, 1380
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 5 cut(s) 229, 882, 1099, 1487, 1533
LmnI GCTCC 2 cut(s) 642, 1450
Lsp1109I GCAGC 2 cut(s) 1101, 1369
LweI GCATC 1 cut(s) 1563
MaeI CTAG 3 cut(s) 533, 711, 1067
MaeII ACGT 5 cut(s) 68, 138, 218, 606, 1051
MaeIII GTNAC 9 cut(s) 7, 64, 82, 568, 602, 1052, 1075, 1235, 1345
MalI GATC 5 cut(s) 231, 884, 1101, 1489, 1535
MboI GATC 5 cut(s) 229, 882, 1099, 1487, 1533
MboII GAAGA 9 cut(s) 349, 397, 605, 843, 1334, 1349, 1367, 1505, 1526
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 1447
MlyI GAGTC 2 cut(s) 500, 1295
MmeI TCCRAC 2 cut(s) 183, 1477
Mph1103I ATGCAT 1 cut(s) 1578
MseI TTAA 4 cut(s) 612, 809, 1025, 1140
MspI CCGG 6 cut(s) 36, 293, 344, 397, 428, 474
MspR9I CCNGG 5 cut(s) 199, 344, 358, 474, 841
MunI CAATTG 1 cut(s) 564
Mva1269I GAATGC 3 cut(s) 19, 1162, 1673
MvaI CCWGG 3 cut(s) 199, 358, 841
MvnI CGCG 2 cut(s) 918, 1380
NciI CCSGG 2 cut(s) 344, 474
NdeII GATC 5 cut(s) 229, 882, 1099, 1487, 1533
NheI GCTAGC 1 cut(s) 532
NlaIII CATG 6 cut(s) 30, 191, 1206, 1227, 1462, 1580
NlaIV GGNNCC 6 cut(s) 40, 416, 644, 833, 974, 1446
NmuCI GTSAC 4 cut(s) 7, 64, 568, 1052
NsiI ATGCAT 1 cut(s) 1578
PagI TCATGA 1 cut(s) 187
PceI AGGCCT 2 cut(s) 844, 1371
PctI GAATGC 3 cut(s) 19, 1162, 1673
PfeI GAWTC 3 cut(s) 32, 848, 1496
PflFI GACNNNGTC 1 cut(s) 1285
PkrI GCNGC 2 cut(s) 1091, 1384
PleI GAGTC 2 cut(s) 500, 1294
PpsI GAGTC 2 cut(s) 500, 1294
PsiI TTATAA 1 cut(s) 1548
Psp6I CCWGG 3 cut(s) 197, 356, 839
PspGI CCWGG 3 cut(s) 197, 356, 839
PspN4I GGNNCC 6 cut(s) 40, 416, 644, 833, 974, 1446
PspPI GGNCC 7 cut(s) 38, 48, 201, 354, 415, 1281, 1336
PsyI GACNNNGTC 1 cut(s) 1285
RsaI GTAC 2 cut(s) 958, 1632
RsaNI GTAC 2 cut(s) 957, 1631
SaqAI TTAA 4 cut(s) 612, 809, 1025, 1140
SatI GCNGC 2 cut(s) 1090, 1383
Sau3AI GATC 5 cut(s) 229, 882, 1099, 1487, 1533
Sau96I GGNCC 7 cut(s) 38, 48, 201, 354, 415, 1281, 1336
SchI GAGTC 2 cut(s) 500, 1295
ScrFI CCNGG 5 cut(s) 199, 344, 358, 474, 841
SduI GDGCHC 1 cut(s) 1447
SfaNI GCATC 1 cut(s) 1563
SinI GGWCC 4 cut(s) 201, 415, 1281, 1336
SmiI ATTTAAAT 1 cut(s) 1026
SmlI CTYRAG 3 cut(s) 152, 391, 1698
SmoI CTYRAG 3 cut(s) 152, 391, 1698
SseBI AGGCCT 2 cut(s) 844, 1371
SsiI CCGC 3 cut(s) 204, 726, 916
SspMI CTAG 3 cut(s) 533, 711, 1067
StuI AGGCCT 2 cut(s) 844, 1371
StyD4I CCNGG 5 cut(s) 197, 342, 356, 472, 839
StyI CCWWGG 1 cut(s) 330
SwaI ATTTAAAT 1 cut(s) 1026
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 5 cut(s) 71, 141, 221, 609, 1054
TaqI TCGA 6 cut(s) 75, 123, 141, 479, 814, 885
TaqII GACCGA 2 cut(s) 211, 1298
TatI WGTACW 1 cut(s) 956
TfiI GAWTC 3 cut(s) 32, 848, 1496
Tru1I TTAA 4 cut(s) 612, 809, 1025, 1140
Tru9I TTAA 4 cut(s) 612, 809, 1025, 1140
TscAI CASTG 2 cut(s) 865, 1407
TseFI GTSAC 4 cut(s) 7, 64, 568, 1052
TseI GCWGC 2 cut(s) 1089, 1382
Tsp45I GTSAC 4 cut(s) 7, 64, 568, 1052
TspDTI ATGAA 6 cut(s) 15, 176, 454, 755, 1258, 1593
TspRI CASTG 2 cut(s) 865, 1407
Tth111I GACNNNGTC 1 cut(s) 1285
VpaK11BI GGWCC 4 cut(s) 201, 415, 1281, 1336
XapI RAATTY 4 cut(s) 746, 754, 1408, 1607
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 1643
XspI CTAG 3 cut(s) 533, 711, 1067
Zsp2I ATGCAT 1 cut(s) 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.