Rroxscaffold_3G00268180

Alpha-trehalose glucohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014435
Physical Location & Seq
Reverse (-)
61314191 .. 61317619
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268180.1

Sequence Viewer

Length: 1710 bp
ATGGTTTTCTCCACCACCATGACTGTCACGAATGCTTCATGCGATTCCGGCCCCGTCAAGCCCACCACCCCGTTGGTCACGTTCCTCGAACAAGTAACACAAACGGCTCTATCCACCTTCGGAAAACCCCACTTCGACCCCAAACTCTACGTCGATTTGGCGCTCAAGTCCGACCTGTCATTGACCCAAGACGCCTTTCATGAGCTTCCCAGGTCCGCAAATGGGTCAACGTCGGTCAATGATCTCAATGAGTTTATACACAACTACTTTGAGGGCGCAGGGGACGATGTGGTGCTCGCTCAGCCGGAGGATTATGTCCCCGAGCCTGAAGGGTTCTTGCCCAAGGTGAAGAACCCGGAAGTGAGGGCCTGGGCTTTGGAGGTTCACTCGCTTTGGAAGAACTTGAGCCGGAAGGTGGCTGGTGGGGTCCACAAGAGGCCGGAGTTTCACACTCTGCTACCTCTGCCTGAGCAGTTCATTATTCCCGGTTCGAGGTTCAGAGAGGTTTATTATTGGGACTCTTATTGGGTTATTAGGGGATTGCTAGCAAGCAAAATGTATGATACAGCAAAAGCAATTGTGACTAATCTCATTTTCCTCATAGAAGAGTATGGTTACGTCCTTAATGGTGCAAGGGCCTATTACACTAACAGGAGCCAACCACCCCTTCTGAGTGCAATGGTTTCCGAAATCTACAAGAGGACAGGAGATTTGGAGTTCGCTAGAAAAGTTCTGCCCGCACTGATCAAAGAACACGAATTCTGGAATTCAGGTATTCATAAGGTAGTTATTCAGGATGACCAAGCTTGTAATCACACTTTAAGTCGATATTATGCAATGTGGAACCAACCCAGGCCTGAATCTTCAACCACTGACAAGGAACTTGCTTCCAAGATCGATAATGTTTCTGAGAAACAGCATTTTTACCGCGAAGTGGCTTCAACTGCTGAGACTGGATGGGATTTCAGTACAAGATGGATGAGGAACCATTCAGATTTCACAACATTGTCCACCACATCCATTTTACCAGTTGATTTAAATGCCTTCATACTGGGAATGGAACGTGACATCGCCTTCCTAGCAAAAGTTACAGGAGACAGCGGCAATTCTGATCACTTCCTGAAGGCTTCGGAAGCAAGACACAAGGCCATTAAATCTGTTTTCTGGAATGCAAAGAAGGGGCAATGGCTTGATTACTGGCTCAATGATAGCTCATGCAATTCAGAACCTCAGACATGGGAAGCTCGTAACCAAAATAAGAATGTATTTGCTTCAAACTTCATCCCTTTGTGGATTGAGTCATTTTACTCAGATACTTCTATGGTCCAGAAAGTTACTACAAGTCTTCAAAGTTCAGGCCTTCTTCGCGCGGCTGGAATTGCAACATCACTGACAAATTCAGGACAACAATGGGACTTTCCAAATGGTTGGGCTCCAATCCAACACATGATAGCTGAAGGTCTGGCAAGGTCTGGATCAAAAGAATCAAAGTTAGTGGGAGAAGAAATTGCTGAAAGGTGGATCAGAACCAATTATAAAGCCTACAAGAAAACAGGTGTGATGCATGAAAAATATGATGTGGAAAAGTGTGGAGAATTTGGAGGTGGTGGTGAATATGTACCCCAGACTGGTTTTGGGTGGTCAAATGGACTTGTATTGGCATTCTTGGAGGAGTTTGGATGGCCTCAAGATAGAAAGATAGATTGCTGA

Protein Analysis

569

Amino Acids

64.41

Weight (kDa)

5.89

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalase PF01204 45 - 560 3.7e-179 Trehalase
MGH1-like_GH PF22422 216 - 535 4.1e-14 Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24040
fragaria_vesca FvH4_5g11460 FvH4_5g11460 FvH4_5g11460 FvH4_5g11460
malus_domestica MD06G1113500.v1.1
prunus_persica Prupe.5G126300_v2.0.a1
pyrus_communis pycom06g10610
rosa_chinensis RchiOBHm_Chr7g0186361
rosa_laevigata RLG00000004848
rosa_multiflora Rmu_sc0000645.1_g000048
rosa_roxburghii Rroxscaffold_3G00268180
rosa_rugosa Rorug06G0473700
rosa_samantha Rh7AG067600 Rh7CG080400 Rh7DG081000
rosa_wichuraiana Rw7G006750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AccII CGCG 3 cut(s) 930, 1368, 1370
AciI CCGC 5 cut(s) 216, 738, 928, 1101, 1370
AclWI GGATC 2 cut(s) 1483, 1529
AcsI RAATTY 4 cut(s) 758, 766, 1396, 1595
AcuI CTGAAG 3 cut(s) 348, 1142, 1476
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 970, 1620
AfiI CCNNNNNNNGG 5 cut(s) 222, 415, 492, 934, 1628
AgsI TTSAA 4 cut(s) 867, 942, 1275, 1349
AjnI CCWGG 3 cut(s) 209, 368, 851
AjuI GAANNNNNNNTTGG 4 cut(s) 180, 212, 651, 683
AluBI AGCT 5 cut(s) 205, 806, 1212, 1244, 1454
AluI AGCT 5 cut(s) 205, 806, 1212, 1244, 1454
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 944, 1089
AlwI GGATC 2 cut(s) 1483, 1529
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 8 cut(s) 49, 366, 437, 636, 854, 1146, 1357, 1682
ApoI RAATTY 4 cut(s) 758, 766, 1396, 1595
ArsI GACNNNNNNTTYG 4 cut(s) 135, 167, 694, 726
AspLEI GCGC 3 cut(s) 163, 278, 1370
AspS9I GGNCC 6 cut(s) 50, 213, 366, 427, 636, 1324
AsuC2I CCSGG 2 cut(s) 356, 486
AsuHPI GGTGA 2 cut(s) 358, 1622
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 3 cut(s) 213, 427, 1324
BanII GRGCYC 1 cut(s) 1435
BbsI GAAGAC 1 cut(s) 1337
Bbv12I GWGCWC 1 cut(s) 297
BccI CCATC 3 cut(s) 951, 969, 1674
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 3 cut(s) 211, 370, 853
BclI TGATCA 2 cut(s) 744, 1111
BcnI CCSGG 2 cut(s) 356, 486
BcoDI GTCTC 2 cut(s) 944, 1089
BfaI CTAG 3 cut(s) 545, 723, 1079
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 2 cut(s) 1102, 1371
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 2 cut(s) 1103, 1372
Bme1390I CCNGG 5 cut(s) 211, 356, 370, 486, 853
Bme18I GGWCC 3 cut(s) 213, 427, 1324
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 6 cut(s) 50, 213, 366, 427, 636, 1324
BmiI GGNNCC 6 cut(s) 52, 428, 656, 845, 986, 1434
BmrFI CCNGG 5 cut(s) 211, 356, 370, 486, 853
BmrI ACTGGG 1 cut(s) 1061
BmsI GCATC 1 cut(s) 1551
BmtI GCTAGC 1 cut(s) 548
BmuI ACTGGG 1 cut(s) 1061
BpiI GAAGAC 1 cut(s) 1337
BplI GAGNNNNNCTC 2 cut(s) 371, 403
Bpu10I CCTNAGC 1 cut(s) 468
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 3 cut(s) 149, 424, 1671
BpuMI CCSGG 2 cut(s) 356, 486
Bsa29I ATCGAT 1 cut(s) 897
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 209, 342, 369, 851
Bsc4I CCNNNNNNNGG 5 cut(s) 222, 415, 492, 934, 1628
Bse1I ACTGG 5 cut(s) 958, 1028, 1056, 1202, 1633
Bse3DI GCAATG 3 cut(s) 684, 843, 1190
BseBI CCWGG 3 cut(s) 211, 370, 853
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 4 cut(s) 209, 342, 369, 851
BseGI GGATG 6 cut(s) 802, 962, 984, 1016, 1281, 1685
BseLI CCNNNNNNNGG 5 cut(s) 222, 415, 492, 934, 1628
BseMI GCAATG 3 cut(s) 684, 843, 1190
BseMII CTCAG 7 cut(s) 314, 459, 662, 900, 939, 1244, 1323
BseNI ACTGG 5 cut(s) 958, 1028, 1056, 1202, 1633
BseRI GAGGAG 1 cut(s) 1685
Bsh1236I CGCG 3 cut(s) 930, 1368, 1370
BshFI GGCC 8 cut(s) 51, 368, 439, 638, 856, 1148, 1359, 1684
BshVI ATCGAT 1 cut(s) 897
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 6 cut(s) 48, 305, 356, 409, 440, 486
BslFI GGGAC 4 cut(s) 296, 302, 530, 1427
BslI CCNNNNNNNGG 5 cut(s) 222, 415, 492, 934, 1628
BsmAI GTCTC 2 cut(s) 944, 1089
BsmFI GGGAC 4 cut(s) 296, 302, 530, 1427
BsmI GAATGC 3 cut(s) 37, 1174, 1661
BsnI GGCC 8 cut(s) 51, 368, 439, 638, 856, 1148, 1359, 1684
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 297, 1435
Bsp143I GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 5 cut(s) 216, 738, 928, 1101, 1370
BspANI GGCC 8 cut(s) 51, 368, 439, 638, 856, 1148, 1359, 1684
BspCNI CTCAG 7 cut(s) 313, 460, 663, 901, 940, 1243, 1322
BspDI ATCGAT 1 cut(s) 897
BspFNI CGCG 3 cut(s) 930, 1368, 1370
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 6 cut(s) 52, 428, 656, 845, 986, 1434
BspOI GCTAGC 1 cut(s) 548
BspPI GGATC 2 cut(s) 1483, 1529
BsrDI GCAATG 3 cut(s) 684, 843, 1190
BsrI ACTGG 5 cut(s) 958, 1028, 1056, 1202, 1633
BssECI CCNNGG 4 cut(s) 209, 342, 369, 851
BssMI GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 3 cut(s) 211, 370, 853
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 600
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 4 cut(s) 297, 546, 550, 738
BstDEI CTNAG 7 cut(s) 300, 468, 671, 909, 948, 1230, 1309
BstF5I GGATG 6 cut(s) 802, 962, 984, 1016, 1281, 1685
BstFNI CGCG 3 cut(s) 930, 1368, 1370
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 3 cut(s) 163, 278, 1370
BstKTI GATC 6 cut(s) 244, 747, 897, 1114, 1478, 1524
BstMAI GTCTC 2 cut(s) 944, 1089
BstMBI GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
BstMWI GCNNNNNNNGC 8 cut(s) 48, 301, 463, 944, 1079, 1133, 1365, 1379
BstNI CCWGG 3 cut(s) 211, 370, 853
BstSCI CCNGG 5 cut(s) 209, 354, 368, 484, 851
BstUI CGCG 3 cut(s) 930, 1368, 1370
BstV2I GAAGAC 1 cut(s) 1337
BstXI CCANNNNNNTGG 2 cut(s) 73, 1428
Bsu15I ATCGAT 1 cut(s) 897
BsuRI GGCC 8 cut(s) 51, 368, 439, 638, 856, 1148, 1359, 1684
BsuTUI ATCGAT 1 cut(s) 897
BtgZI GCGATG 1 cut(s) 1054
BtsCI GGATG 6 cut(s) 802, 962, 984, 1016, 1281, 1685
BtsIMutI CAGTG 3 cut(s) 740, 870, 1388
Cac8I GCNNGC 4 cut(s) 297, 546, 550, 738
CciI TCATGA 1 cut(s) 199
CfoI GCGC 3 cut(s) 163, 278, 1370
Cfr13I GGNCC 6 cut(s) 50, 213, 366, 427, 636, 1324
ClaI ATCGAT 1 cut(s) 897
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 969, 1619
CspCI CAANNNNNGTGG 2 cut(s) 1476, 1511
CviAII CATG 7 cut(s) 19, 39, 200, 1215, 1236, 1447, 1565
CviQI GTAC 2 cut(s) 969, 1619
DdeI CTNAG 7 cut(s) 300, 468, 671, 909, 948, 1230, 1309
DpnI GATC 6 cut(s) 243, 746, 896, 1113, 1477, 1523
DpnII GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
DraI TTTAAA 1 cut(s) 1038
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 342
Eco147I AGGCCT 2 cut(s) 856, 1359
Eco24I GRGCYC 1 cut(s) 1435
Eco47I GGWCC 3 cut(s) 213, 427, 1324
Eco57I CTGAAG 3 cut(s) 348, 1142, 1476
Eco88I CYCGRG 1 cut(s) 320
EcoO109I RGGNCCY 2 cut(s) 366, 636
EcoRI GAATTC 2 cut(s) 758, 766
EcoRII CCWGG 3 cut(s) 209, 368, 851
EcoT14I CCWWGG 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 1566
EcoT38I GRGCYC 1 cut(s) 1435
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 7 cut(s) 22, 42, 203, 1218, 1239, 1450, 1568
FaqI GGGAC 4 cut(s) 296, 302, 530, 1427
FatI CATG 7 cut(s) 18, 38, 199, 1214, 1235, 1446, 1564
FauI CCCGC 1 cut(s) 745
FbaI TGATCA 2 cut(s) 744, 1111
Fnu4HI GCNGC 2 cut(s) 1102, 1371
FokI GGATG 6 cut(s) 809, 969, 991, 1003, 1268, 1692
FriOI GRGCYC 1 cut(s) 1435
Fsp4HI GCNGC 2 cut(s) 1102, 1371
FspBI CTAG 3 cut(s) 545, 723, 1079
GlaI GCGC 3 cut(s) 162, 277, 1369
GluI GCNGC 2 cut(s) 1102, 1371
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 8 cut(s) 51, 368, 439, 638, 856, 1148, 1359, 1684
HapII CCGG 6 cut(s) 48, 305, 356, 409, 440, 486
HgaI GACGC 1 cut(s) 200
HhaI GCGC 3 cut(s) 163, 278, 1370
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 7 cut(s) 22, 42, 203, 1218, 1239, 1450, 1568
Hin6I GCGC 3 cut(s) 161, 276, 1368
HinP1I GCGC 3 cut(s) 161, 276, 1368
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HindIII AAGCTT 1 cut(s) 804
HinfI GANTC 5 cut(s) 44, 518, 860, 1298, 1484
HpaII CCGG 6 cut(s) 48, 305, 356, 409, 440, 486
HphI GGTGA 2 cut(s) 358, 1622
Hpy166II GTNNAC 4 cut(s) 228, 385, 430, 1011
Hpy8I GTNNAC 4 cut(s) 228, 385, 430, 1011
Hpy99I CGWCG 2 cut(s) 155, 235
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 5 cut(s) 80, 150, 230, 618, 1063
HpyCH4V TGCA 7 cut(s) 632, 677, 836, 1172, 1218, 1382, 1564
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 301, 463, 944, 1079, 1133, 1365, 1379
HpyF3I CTNAG 7 cut(s) 300, 468, 671, 909, 948, 1230, 1309
HpySE526I ACGT 5 cut(s) 80, 150, 230, 618, 1063
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 7 cut(s) 22, 42, 203, 1218, 1239, 1450, 1568
HspAI GCGC 3 cut(s) 161, 276, 1368
Ksp22I TGATCA 2 cut(s) 744, 1111
Kzo9I GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
LmnI GCTCC 2 cut(s) 654, 1438
LweI GCATC 1 cut(s) 1551
MaeI CTAG 3 cut(s) 545, 723, 1079
MaeII ACGT 5 cut(s) 80, 150, 230, 618, 1063
MaeIII GTNAC 9 cut(s) 25, 76, 94, 580, 614, 1064, 1087, 1247, 1333
MalI GATC 6 cut(s) 243, 746, 896, 1113, 1477, 1523
MboI GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
MboII GAAGA 7 cut(s) 361, 409, 617, 855, 1337, 1355, 1514
MfeI CAATTG 1 cut(s) 576
MhlI GDGCHC 2 cut(s) 297, 1435
MlyI GAGTC 2 cut(s) 512, 1307
MmeI TCCRAC 2 cut(s) 195, 1465
Mph1103I ATGCAT 1 cut(s) 1566
MseI TTAA 4 cut(s) 624, 821, 1037, 1152
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 1101
MspI CCGG 6 cut(s) 48, 305, 356, 409, 440, 486
MspR9I CCNGG 5 cut(s) 211, 356, 370, 486, 853
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 3 cut(s) 37, 1174, 1661
MvaI CCWGG 3 cut(s) 211, 370, 853
MvnI CGCG 3 cut(s) 930, 1368, 1370
MwoI GCNNNNNNNGC 8 cut(s) 48, 301, 463, 944, 1079, 1133, 1365, 1379
NciI CCSGG 2 cut(s) 356, 486
NdeII GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
NheI GCTAGC 1 cut(s) 544
NlaIII CATG 7 cut(s) 22, 42, 203, 1218, 1239, 1450, 1568
NlaIV GGNNCC 6 cut(s) 52, 428, 656, 845, 986, 1434
NmuCI GTSAC 4 cut(s) 25, 76, 580, 1064
NsiI ATGCAT 1 cut(s) 1566
PagI TCATGA 1 cut(s) 199
PceI AGGCCT 2 cut(s) 856, 1359
PctI GAATGC 3 cut(s) 37, 1174, 1661
PfeI GAWTC 3 cut(s) 44, 860, 1484
PkrI GCNGC 2 cut(s) 1103, 1372
PleI GAGTC 2 cut(s) 512, 1306
PpsI GAGTC 2 cut(s) 512, 1306
PsiI TTATAA 1 cut(s) 1536
Psp6I CCWGG 3 cut(s) 209, 368, 851
PspGI CCWGG 3 cut(s) 209, 368, 851
PspN4I GGNNCC 6 cut(s) 52, 428, 656, 845, 986, 1434
PspPI GGNCC 6 cut(s) 50, 213, 366, 427, 636, 1324
RsaI GTAC 2 cut(s) 970, 1620
RsaNI GTAC 2 cut(s) 969, 1619
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 4 cut(s) 624, 821, 1037, 1152
SatI GCNGC 2 cut(s) 1102, 1371
Sau3AI GATC 6 cut(s) 241, 744, 894, 1111, 1475, 1521
Sau96I GGNCC 6 cut(s) 50, 213, 366, 427, 636, 1324
SchI GAGTC 2 cut(s) 512, 1307
ScrFI CCNGG 5 cut(s) 211, 356, 370, 486, 853
SduI GDGCHC 2 cut(s) 297, 1435
SfaNI GCATC 1 cut(s) 1551
SinI GGWCC 3 cut(s) 213, 427, 1324
SmiI ATTTAAAT 1 cut(s) 1038
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 3 cut(s) 164, 403, 1686
SmoI CTYRAG 3 cut(s) 164, 403, 1686
SseBI AGGCCT 2 cut(s) 856, 1359
SsiI CCGC 5 cut(s) 216, 738, 928, 1101, 1370
SspMI CTAG 3 cut(s) 545, 723, 1079
StuI AGGCCT 2 cut(s) 856, 1359
StyD4I CCNGG 5 cut(s) 209, 354, 368, 484, 851
StyI CCWWGG 1 cut(s) 342
SwaI ATTTAAAT 1 cut(s) 1038
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 5 cut(s) 83, 153, 233, 621, 1066
TaqI TCGA 6 cut(s) 87, 135, 153, 491, 826, 897
TaqII GACCGA 1 cut(s) 223
TatI WGTACW 1 cut(s) 968
TauI GCSGC 2 cut(s) 1104, 1373
TfiI GAWTC 3 cut(s) 44, 860, 1484
Tru1I TTAA 4 cut(s) 624, 821, 1037, 1152
Tru9I TTAA 4 cut(s) 624, 821, 1037, 1152
TscAI CASTG 3 cut(s) 747, 877, 1395
TseFI GTSAC 4 cut(s) 25, 76, 580, 1064
Tsp45I GTSAC 4 cut(s) 25, 76, 580, 1064
TspDTI ATGAA 7 cut(s) 27, 188, 466, 767, 1036, 1270, 1581
TspRI CASTG 3 cut(s) 747, 877, 1395
VpaK11BI GGWCC 3 cut(s) 213, 427, 1324
XapI RAATTY 4 cut(s) 758, 766, 1396, 1595
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 1631
XspI CTAG 3 cut(s) 545, 723, 1079
Zsp2I ATGCAT 1 cut(s) 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.