MD06G1120400.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015993
Physical Location & Seq
Forward (+)
26067917 .. 26070086
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1120400.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGAGGCCCCTAAGATTTCTCATTTCCTTCTTCTATGTGCAATCTGCCCAAATTTTGTTGTGCTTTTGAGTGCCTTTCCATGTTTTGCTTTCACTTCATTTGTGTTTGGAGATTCTCTAGTGGACGCAGGAAACAATGACTACATTTTCACACTCTCAAAGGCTGATTCTCCTCCTTATGGAATAGATTTCAAGCCTTCTGGTGGTCAACCAACTGGTCGATTCACAAATGGTCGAACCGTATCGGACATTGTAGGTCAAGCTCTTGGTGCTAAGTCATTTCCTCCACCTTATCTAGCCCCAAACACTCGAGCAAACTCAGTCCTCAAAGGAATTAATTATGCTTCTGGAGCCTCAGGAATATTGGATGAAACAGGAGTTTTGTTTATTGGAAGAGTCCCACTGCGCGAGCAAGTGAATAATTTTGAGGAAAGCAGATATGACATGGTGAAAATAATGGGAGAGAATAAAACAAAGGAATTCTTAAAGAAGGCAATCTTCTCGGTGACAATTGGATCCAATGATGTTCTCAACTATTTCCAACCGACCATACCATTTTTTGGTGATGACAAGGTCTCTCCTTCCATGTTCCAAGATTTCATGGTCTCTAACTTGACCATACAGCTTAAGCGACTGCACGAGCTGGGAGCTAGAAAGTTCGTTGTGGTAGGGGTTGGACCCATAGGATGCATACCATTTATTCGTGCCATCCATTTGTTACCAAGTGGACAGTGTTTTGCTGAGGTGAATGAATTAATCCAAGGCTACAACACGAAGCTTAATGGAGTTTTGGATCAAATTAACCAAGAGCTGGGTCCTGAAGCGATCTTTGTCTATGCAAACTCCTTTGATATCTTCACGAAGATCATAGTAAATTATCATCAATATGGTAGGTTGGTAACTAGGGTTTTAAAAGTAGACATACCCTTCCATTCGTTTTTAGTATATGATCAAGGGTTTGCGAATGCAAATGGACCATGCTGTGGAGGTTACTTTCCGCCGTTTGTTTGCTTTAAGAGCCGCGATGCAAGCAGAAGCTCTGCATTGTGTGACGATCGATCGAAGTACGTGTTTTGGGATGCATATCATCCTACAGAAGCTGCTAATATGATCATAGCAGAGGGGTTACTTGATGGAGATAAAAGTATTAGCTCTCCCATTAACATCCGTGAGCTCTACAACTATAACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

398

Amino Acids

43.85

Weight (kDa)

6.1

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 375 3.9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41890
fragaria_vesca FvH4_5g10830
malus_domestica MD06G1120400.v1.1
prunus_persica Prupe.5G130900_v2.0.a1
pyrus_communis pycom06g11170
rosa_chinensis RchiOBHm_Chr7g0187331
rosa_laevigata RLG00000004794
rosa_multiflora Rmu_sc0001758.1_g000012
rosa_roxburghii Rroxscaffold_3G00267600
rosa_rugosa Rorug06G0480600.1
rosa_samantha Rh7AG085100 Rh7BG077500 Rh7CG086100 Rh7DG087200
rosa_wichuraiana Rw7G007480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 560, 811, 983
AccI GTMKAC 1 cut(s) 918
AccII CGCG 2 cut(s) 408, 1023
AciI CCGC 2 cut(s) 998, 1021
AclWI GGATC 3 cut(s) 510, 523, 801
AcsI RAATTY 2 cut(s) 52, 479
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 1 cut(s) 1067
AfiI CCNNNNNNNGG 5 cut(s) 179, 203, 560, 811, 983
AflII CTTAAG 1 cut(s) 626
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 1 cut(s) 193
Alw21I GWGCWC 1 cut(s) 1176
Alw26I GTCTC 2 cut(s) 580, 610
AlwI GGATC 3 cut(s) 510, 523, 801
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 1 cut(s) 1100
ApoI RAATTY 2 cut(s) 52, 479
AseI ATTAAT 2 cut(s) 336, 755
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 4 cut(s) 7, 677, 815, 974
AsuHPI GGTGA 4 cut(s) 460, 517, 575, 757
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 3 cut(s) 677, 815, 974
AxyI CCTNAGG 1 cut(s) 355
BamHI GGATCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 1176
BauI CACGAG 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 1176
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 1 cut(s) 1087
BccI CCATC 2 cut(s) 716, 1127
BceAI ACGGC 1 cut(s) 985
BcgI CGANNNNNNTGC 2 cut(s) 483, 517
BclI TGATCA 2 cut(s) 949, 1110
BcoDI GTCTC 2 cut(s) 580, 610
BfaI CTAG 5 cut(s) 119, 296, 651, 903, 1192
BfmI CTRYAG 1 cut(s) 1092
BfrI CTTAAG 1 cut(s) 626
BisI GCNGC 2 cut(s) 1021, 1101
BlsI GCNGC 2 cut(s) 1022, 1102
Bme18I GGWCC 3 cut(s) 677, 815, 974
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 4 cut(s) 7, 677, 815, 974
BmiI GGNNCC 5 cut(s) 9, 352, 517, 679, 816
BmsI GCATC 3 cut(s) 677, 1015, 1069
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 741
Bsa29I ATCGAT 1 cut(s) 1057
BsaAI YACGTR 1 cut(s) 1069
BsaBI GATNNNNATC 1 cut(s) 1083
BsaI GGTCTC 2 cut(s) 580, 610
BsaJI CCNNGG 1 cut(s) 760
BsaXI ACNNNNNCTCC 1 cut(s) 1172
Bsc4I CCNNNNNNNGG 5 cut(s) 179, 203, 560, 811, 983
Bse1I ACTGG 1 cut(s) 220
Bse21I CCTNAGG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 1083
BseCI ATCGAT 1 cut(s) 1057
BseDI CCNNGG 1 cut(s) 760
BseGI GGATG 6 cut(s) 373, 692, 708, 1084, 1087, 1164
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 5 cut(s) 179, 203, 560, 811, 983
BseMII CTCAG 3 cut(s) 333, 369, 732
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 1087
BseYI CCCAGC 2 cut(s) 643, 811
BsgI GTGCAG 1 cut(s) 620
Bsh1236I CGCG 2 cut(s) 408, 1023
Bsh1285I CGRYCG 2 cut(s) 1057, 1061
BshFI GGCC 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 1057
BsiEI CGRYCG 2 cut(s) 1057, 1061
BsiHKAI GWGCWC 1 cut(s) 1176
BsiHKCI CYCGRG 1 cut(s) 309
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 5 cut(s) 179, 203, 560, 811, 983
BsmAI GTCTC 2 cut(s) 580, 610
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 970
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 2 cut(s) 580, 610
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 1176
Bsp143I GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
BspACI CCGC 2 cut(s) 998, 1021
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 3 cut(s) 332, 368, 733
BspDI ATCGAT 1 cut(s) 1057
BspFNI CGCG 2 cut(s) 408, 1023
BspLI GGNNCC 5 cut(s) 9, 352, 517, 679, 816
BspPI GGATC 3 cut(s) 510, 523, 801
BspTI CTTAAG 1 cut(s) 626
BspTNI GGTCTC 2 cut(s) 580, 610
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 760
BssMI GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 1 cut(s) 760
Bst2BI CACGAG 1 cut(s) 638
Bst4CI ACNGT 2 cut(s) 241, 732
Bst6I CTCTTC 1 cut(s) 388
BstAFI CTTAAG 1 cut(s) 626
BstBAI YACGTR 1 cut(s) 1069
BstC8I GCNNGC 2 cut(s) 410, 1030
BstDEI CTNAG 5 cut(s) 12, 273, 319, 355, 741
BstF5I GGATG 6 cut(s) 373, 692, 708, 1084, 1087, 1164
BstFNI CGCG 2 cut(s) 408, 1023
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 8 cut(s) 518, 796, 828, 867, 952, 1057, 1061, 1113
BstMAI GTCTC 2 cut(s) 580, 610
BstMBI GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
BstMCI CGRYCG 2 cut(s) 1057, 1061
BstMWI GCNNNNNNNGC 4 cut(s) 269, 350, 1017, 1029
BstSFI CTRYAG 1 cut(s) 1092
BstUI CGCG 2 cut(s) 408, 1023
BstV1I GCAGC 1 cut(s) 1087
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
Bsu15I ATCGAT 1 cut(s) 1057
Bsu36I CCTNAGG 1 cut(s) 355
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 1057
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 6 cut(s) 373, 692, 708, 1084, 1087, 1164
BtsI GCAGTG 1 cut(s) 401
BtsIMutI CAGTG 2 cut(s) 401, 737
Cac8I GCNNGC 2 cut(s) 410, 1030
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 4 cut(s) 7, 677, 815, 974
ClaI ATCGAT 1 cut(s) 1057
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 1066
CviAII CATG 5 cut(s) 81, 445, 586, 601, 978
CviQI GTAC 1 cut(s) 1066
DdeI CTNAG 5 cut(s) 12, 273, 319, 355, 741
DpnI GATC 8 cut(s) 517, 795, 827, 866, 951, 1056, 1060, 1112
DpnII GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
DraI TTTAAA 1 cut(s) 912
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
EciI GGCGGA 1 cut(s) 987
Ecl136II GAGCTC 1 cut(s) 1174
Eco130I CCWWGG 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 1176
Eco31I GGTCTC 2 cut(s) 580, 610
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 3 cut(s) 677, 815, 974
Eco53kI GAGCTC 1 cut(s) 1174
Eco57I CTGAAG 1 cut(s) 840
Eco81I CCTNAGG 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 1174
EcoO109I RGGNCCY 2 cut(s) 7, 815
EcoRI GAATTC 1 cut(s) 479
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 760
EcoT22I ATGCAT 2 cut(s) 692, 1084
EcoT38I GRGCYC 1 cut(s) 1176
ErhI CCWWGG 1 cut(s) 760
FaeI CATG 5 cut(s) 84, 448, 589, 604, 981
FalI AAGNNNNNCTT 4 cut(s) 467, 499, 482, 514
FaqI GGGAC 1 cut(s) 383
FatI CATG 5 cut(s) 80, 444, 585, 600, 977
FbaI TGATCA 2 cut(s) 949, 1110
FblI GTMKAC 1 cut(s) 918
Fnu4HI GCNGC 2 cut(s) 1021, 1101
FokI GGATG 6 cut(s) 380, 695, 699, 1074, 1091, 1151
FriOI GRGCYC 1 cut(s) 1176
Fsp4HI GCNGC 2 cut(s) 1021, 1101
FspBI CTAG 5 cut(s) 119, 296, 651, 903, 1192
GlaI GCGC 1 cut(s) 407
GluI GCNGC 2 cut(s) 1021, 1101
GsaI CCCAGC 2 cut(s) 647, 815
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 1 cut(s) 8
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 408
Hin1II CATG 5 cut(s) 84, 448, 589, 604, 981
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 4 cut(s) 113, 167, 222, 396
HphI GGTGA 4 cut(s) 460, 517, 575, 757
Hpy166II GTNNAC 4 cut(s) 124, 209, 728, 919
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 4 cut(s) 348, 357, 818, 859
Hpy8I GTNNAC 4 cut(s) 124, 209, 728, 919
HpyAV CCTTC 5 cut(s) 38, 207, 484, 591, 937
HpyCH4III ACNGT 2 cut(s) 241, 732
HpyCH4IV ACGT 1 cut(s) 1068
HpyCH4V TGCA 8 cut(s) 41, 637, 690, 839, 968, 1028, 1043, 1082
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 350, 1017, 1029
HpyF3I CTNAG 5 cut(s) 12, 273, 319, 355, 741
HpySE526I ACGT 1 cut(s) 1068
Hsp92II CATG 5 cut(s) 84, 448, 589, 604, 981
HspAI GCGC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 949, 1110
Kzo9I GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
LmnI GCTCC 2 cut(s) 350, 647
LpnPI CCDG 9 cut(s) 114, 186, 201, 333, 342, 360, 629, 797, 831
Lsp1109I GCAGC 1 cut(s) 1087
LweI GCATC 3 cut(s) 677, 1015, 1069
MaeI CTAG 5 cut(s) 119, 296, 651, 903, 1192
MaeII ACGT 1 cut(s) 1068
MaeIII GTNAC 6 cut(s) 505, 717, 898, 989, 1049, 1125
MalI GATC 8 cut(s) 517, 795, 827, 866, 951, 1056, 1060, 1112
MboI GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
MboII GAAGA 5 cut(s) 23, 405, 490, 847, 874
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 1176
MluCI AATT 9 cut(s) 52, 333, 337, 421, 479, 510, 752, 798, 874
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 2 cut(s) 565, 655
MnlI CCTC 8 cut(s) 183, 294, 335, 364, 421, 736, 980, 1114
Mph1103I ATGCAT 2 cut(s) 692, 1084
MseI TTAA 9 cut(s) 336, 485, 627, 755, 780, 801, 911, 1014, 1161
MslI CAYNNNNRTG 1 cut(s) 885
MspCI CTTAAG 1 cut(s) 626
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 970
MvnI CGCG 2 cut(s) 408, 1023
MwoI GCNNNNNNNGC 4 cut(s) 269, 350, 1017, 1029
NdeII GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
NlaIII CATG 5 cut(s) 84, 448, 589, 604, 981
NlaIV GGNNCC 5 cut(s) 9, 352, 517, 679, 816
NmuCI GTSAC 2 cut(s) 505, 1049
NsiI ATGCAT 2 cut(s) 692, 1084
PaeR7I CTCGAG 1 cut(s) 309
PctI GAATGC 1 cut(s) 970
PfeI GAWTC 3 cut(s) 113, 167, 222
PflFI GACNNNGTC 1 cut(s) 572
PflMI CCANNNNNTGG 3 cut(s) 560, 811, 983
PkrI GCNGC 2 cut(s) 1022, 1102
Ple19I CGATCG 2 cut(s) 1057, 1061
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Ppu21I YACGTR 1 cut(s) 1069
PpuMI RGGWCCY 1 cut(s) 815
PshBI ATTAAT 2 cut(s) 336, 755
Psp124BI GAGCTC 1 cut(s) 1176
Psp5II RGGWCCY 1 cut(s) 815
PspFI CCCAGC 2 cut(s) 643, 811
PspN4I GGNNCC 5 cut(s) 9, 352, 517, 679, 816
PspPI GGNCC 4 cut(s) 7, 677, 815, 974
PspPPI RGGWCCY 1 cut(s) 815
PspXI VCTCGAGB 1 cut(s) 309
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 1 cut(s) 515
PsyI GACNNNGTC 1 cut(s) 572
PvuI CGATCG 2 cut(s) 1057, 1061
RsaI GTAC 1 cut(s) 1067
RsaNI GTAC 1 cut(s) 1066
RseI CAYNNNNRTG 1 cut(s) 885
SacI GAGCTC 1 cut(s) 1176
SaqAI TTAA 9 cut(s) 336, 485, 627, 755, 780, 801, 911, 1014, 1161
SatI GCNGC 2 cut(s) 1021, 1101
Sau3AI GATC 8 cut(s) 515, 793, 825, 864, 949, 1054, 1058, 1110
Sau96I GGNCC 4 cut(s) 7, 677, 815, 974
SchI GAGTC 1 cut(s) 405
SduI GDGCHC 1 cut(s) 1176
SfaNI GCATC 3 cut(s) 677, 1015, 1069
SfcI CTRYAG 1 cut(s) 1092
Sfr274I CTCGAG 1 cut(s) 309
SinI GGWCC 3 cut(s) 677, 815, 974
SlaI CTCGAG 1 cut(s) 309
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 2 cut(s) 309, 626
SmoI CTYRAG 2 cut(s) 309, 626
Sse9I AATT 9 cut(s) 52, 333, 337, 421, 479, 510, 752, 798, 874
SsiI CCGC 2 cut(s) 998, 1021
SspI AATATT 1 cut(s) 363
SspMI CTAG 5 cut(s) 119, 296, 651, 903, 1192
SstI GAGCTC 1 cut(s) 1176
StyI CCWWGG 1 cut(s) 760
TaaI ACNGT 2 cut(s) 241, 732
TaiI ACGT 1 cut(s) 1071
TaqI TCGA 5 cut(s) 220, 235, 310, 1057, 1061
TasI AATT 9 cut(s) 52, 333, 337, 421, 479, 510, 752, 798, 874
TauI GCSGC 1 cut(s) 1023
TfiI GAWTC 3 cut(s) 113, 167, 222
Tru1I TTAA 9 cut(s) 336, 485, 627, 755, 780, 801, 911, 1014, 1161
Tru9I TTAA 9 cut(s) 336, 485, 627, 755, 780, 801, 911, 1014, 1161
TscAI CASTG 2 cut(s) 408, 737
TseFI GTSAC 2 cut(s) 505, 1049
TseI GCWGC 1 cut(s) 1100
Tsp45I GTSAC 2 cut(s) 505, 1049
TspDTI ATGAA 4 cut(s) 87, 384, 589, 765
TspGWI ACGGA 1 cut(s) 1157
TspRI CASTG 2 cut(s) 408, 737
Tth111I GACNNNGTC 1 cut(s) 572
Van91I CCANNNNNTGG 3 cut(s) 560, 811, 983
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 3 cut(s) 677, 815, 974
VspI ATTAAT 2 cut(s) 336, 755
XapI RAATTY 2 cut(s) 52, 479
XhoI CTCGAG 1 cut(s) 309
XmiI GTMKAC 1 cut(s) 918
XspI CTAG 5 cut(s) 119, 296, 651, 903, 1192
Zsp2I ATGCAT 2 cut(s) 692, 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.