Rroxscaffold_3G00267600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015993
Physical Location & Seq
Reverse (-)
60681122 .. 60683793
2672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267600.1

Sequence Viewer

Length: 1074 bp
ATGGAGGCCCTTCAGAAAACTTGTCAGTCTATTCTTCTATGTACAGTTTTCATTGTTCTTTGTTTGGAGGCTTCCCATTGCTTTGCTTTCACTTCGTTCGTGTTTGGGGACTCTCTGGTTGATGCAGGAAACAATGACTACATCTTCACGCTCTCGAAAGCTGACTCTCCTCCTTATGGCATCGATTTTAAGGCTTCAGGTGGACGACCCACCGGTAGGTTCACAAATGGTCGAACCATATCTGACATCATAGATTGGAAGAGTCCACTGCGTGAGCAAGTAAATAATTTTGAGCAGAGCAGAAGTTACATGGTAAACGTAATGGGAGAGAGAAGCACAAGAGAATTTCTAAAGAAGGCAATCTTCTCGATCACAATTGGATCCAACGATGTTCTCAACTATGTCCAACCATCCATACCATTTTTTGGTCATGACAAAGTCTCACCCAACACGTTCCAACATTTCATGGTCTCTAACTTGACAATACAACTCAAGCGACTGCACGAGTTGGGAGCTAGAAAGTTCATTGTGGTTGGAGTTGGACCATTGGGTTGCATACCCTTCGTTCGTGCCGTTAATCTCTTACCAAGTGGTCACTGCTTTGGTGAAGTGAATGATTTGATCCAAGGCTACAATAAGAAACTCAATGGTTTGTTGGATCGACTGAACCAGGAGTTGGGTCCTGAAGCTATCTTTGTGTATGCAAATTCTTATGACATCTTCATGAAGATCATAGTGAATTATCATCAATATGGTAAAACTAGGGCTAACCTTTTGTTTATTTTTATTATTGAGATCGTTTATTTTGATCTGTTTCGATATGTTTGGTATTTGCTAGGGTTTAAGGATGCGGATGCTCCTTGTTGTGGTGGATACTTTCCACTATTTCTTTGCTTCATGGGAAGGAACACAAGCTCTCCCCTGTGTGGTGATCGATCCAAGTATGTGTTTTGGGATGCTTATCATCCTACAGAGGCTGCTAATGTTATCATAGCTAAGAGGTTGCTTGATGGAGATGAAAGTATTAGCTCACCTATTAACATCCGGGAGCTTTATAACTACAATTCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

40.42

Weight (kDa)

6.66

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 335 4.1e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41890
fragaria_vesca FvH4_5g10830
malus_domestica MD06G1120400.v1.1
prunus_persica Prupe.5G130900_v2.0.a1
pyrus_communis pycom06g11170
rosa_chinensis RchiOBHm_Chr7g0187331
rosa_laevigata RLG00000004794
rosa_multiflora Rmu_sc0001758.1_g000012
rosa_roxburghii Rroxscaffold_3G00267600
rosa_rugosa Rorug06G0480600.1
rosa_samantha Rh7AG085100 Rh7BG077500 Rh7CG086100 Rh7DG087200
rosa_wichuraiana Rw7G007480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1056
AccB7I CCANNNNNTGG 2 cut(s) 425, 676
AciI CCGC 1 cut(s) 851
AclWI GGATC 5 cut(s) 375, 388, 616, 666, 930
AcsI RAATTY 2 cut(s) 344, 706
AcuI CTGAAG 2 cut(s) 180, 705
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 6 cut(s) 176, 216, 425, 676, 866, 926
AflIII ACRYGT 1 cut(s) 450
AgeI ACCGGT 1 cut(s) 212
AjnI CCWGG 1 cut(s) 669
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AluBI AGCT 7 cut(s) 161, 515, 689, 915, 995, 1029, 1051
AluI AGCT 7 cut(s) 161, 515, 689, 915, 995, 1029, 1051
Alw26I GTCTC 2 cut(s) 445, 475
AlwI GGATC 5 cut(s) 375, 388, 616, 666, 930
AlwNI CAGNNNCTG 1 cut(s) 977
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 1 cut(s) 977
ApoI RAATTY 2 cut(s) 344, 706
AsiGI ACCGGT 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 7, 542, 680
AsuC2I CCSGG 1 cut(s) 1046
AsuHPI GGTGA 4 cut(s) 435, 617, 941, 1023
AvaII GGWCC 2 cut(s) 542, 680
BamHI GGATCC 1 cut(s) 380
BauI CACGAG 1 cut(s) 503
BbvI GCAGC 1 cut(s) 964
BccI CCATC 2 cut(s) 418, 1004
BceAI ACGGC 1 cut(s) 557
BcgI CGANNNNNNTGC 4 cut(s) 348, 382, 544, 578
BciT130I CCWGG 1 cut(s) 671
BciVI GTATCC 1 cut(s) 866
BcnI CCSGG 1 cut(s) 1046
BcoDI GTCTC 2 cut(s) 445, 475
BfaI CTAG 3 cut(s) 516, 762, 836
BfmI CTRYAG 1 cut(s) 969
BfuI GTATCC 1 cut(s) 866
BisI GCNGC 1 cut(s) 978
BlsI GCNGC 1 cut(s) 979
Bme1390I CCNGG 2 cut(s) 671, 1046
Bme18I GGWCC 2 cut(s) 542, 680
BmgT120I GGNCC 3 cut(s) 7, 542, 680
BmiI GGNNCC 2 cut(s) 382, 681
BmrFI CCNGG 2 cut(s) 671, 1046
BmsI GCATC 5 cut(s) 112, 189, 838, 844, 946
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 1046
Bsa29I ATCGAT 2 cut(s) 183, 934
BsaBI GATNNNNATC 1 cut(s) 960
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 625
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 216, 425, 676, 866, 926
Bse118I RCCGGY 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 1 cut(s) 671
BseCI ATCGAT 2 cut(s) 183, 934
BseDI CCNNGG 1 cut(s) 625
BseGI GGATG 6 cut(s) 410, 853, 859, 961, 964, 1041
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 6 cut(s) 176, 216, 425, 676, 866, 926
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 1059
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 964
BsgI GTGCAG 1 cut(s) 485
BshFI GGCC 1 cut(s) 8
BshTI ACCGGT 1 cut(s) 212
BshVI ATCGAT 2 cut(s) 183, 934
BsiSI CCGG 2 cut(s) 213, 1045
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 6 cut(s) 176, 216, 425, 676, 866, 926
BsmAI GTCTC 2 cut(s) 445, 475
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 41
Bsp143I GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
BspACI CCGC 1 cut(s) 851
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 1060
BspDI ATCGAT 2 cut(s) 183, 934
BspHI TCATGA 2 cut(s) 430, 723
BspLI GGNNCC 2 cut(s) 382, 681
BspPI GGATC 5 cut(s) 375, 388, 616, 666, 930
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 625
BssMI GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 1 cut(s) 625
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 254
BstAUI TGTACA 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 996, 1068
BstF5I GGATG 6 cut(s) 410, 853, 859, 961, 964, 1041
BstKTI GATC 9 cut(s) 372, 383, 624, 661, 732, 798, 811, 934, 938
BstMAI GTCTC 2 cut(s) 445, 475
BstMBI GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
BstNI CCWGG 1 cut(s) 671
BstSCI CCNGG 2 cut(s) 669, 1044
BstSFI CTRYAG 1 cut(s) 969
BstV1I GCAGC 1 cut(s) 964
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
Bsu15I ATCGAT 2 cut(s) 183, 934
BsuI GTATCC 1 cut(s) 866
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 2 cut(s) 183, 934
BtsCI GGATG 6 cut(s) 410, 853, 859, 961, 964, 1041
BtsI GCAGTG 2 cut(s) 266, 595
BtsIMutI CAGTG 2 cut(s) 266, 595
CaiI CAGNNNCTG 1 cut(s) 977
CciI TCATGA 2 cut(s) 430, 723
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 7, 542, 680
ClaI ATCGAT 2 cut(s) 183, 934
Csp6I GTAC 1 cut(s) 42
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 5 cut(s) 310, 431, 466, 724, 898
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 2 cut(s) 996, 1068
DpnI GATC 9 cut(s) 371, 382, 623, 660, 731, 797, 810, 933, 937
DpnII GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
DraIII CACNNNGTG 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 542, 680
Eco57I CTGAAG 2 cut(s) 180, 705
EcoO109I RGGNCCY 2 cut(s) 7, 680
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 625
FaeI CATG 5 cut(s) 313, 434, 469, 727, 901
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 122
FatI CATG 5 cut(s) 309, 430, 465, 723, 897
Fnu4HI GCNGC 1 cut(s) 978
FokI GGATG 6 cut(s) 397, 860, 866, 951, 968, 1028
Fsp4HI GCNGC 1 cut(s) 978
FspBI CTAG 3 cut(s) 516, 762, 836
GluI GCNGC 1 cut(s) 978
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 213, 1045
Hin1II CATG 5 cut(s) 313, 434, 469, 727, 901
HinfI GANTC 3 cut(s) 110, 164, 262
HpaII CCGG 2 cut(s) 213, 1045
HphI GGTGA 4 cut(s) 435, 617, 941, 1023
Hpy166II GTNNAC 4 cut(s) 203, 222, 266, 316
Hpy188I TCNGA 3 cut(s) 15, 244, 1069
Hpy188III TCNNGA 5 cut(s) 154, 367, 431, 683, 724
Hpy8I GTNNAC 4 cut(s) 203, 222, 266, 316
HpyAV CCTTC 4 cut(s) 20, 349, 571, 897
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 2 cut(s) 318, 452
HpyCH4V TGCA 4 cut(s) 125, 502, 555, 704
HpyF3I CTNAG 2 cut(s) 996, 1068
HpySE526I ACGT 2 cut(s) 318, 452
Hsp92II CATG 5 cut(s) 313, 434, 469, 727, 901
Kzo9I GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
LmnI GCTCC 3 cut(s) 512, 862, 1048
LpnPI CCDG 9 cut(s) 101, 111, 183, 226, 656, 683, 696, 935, 1058
Lsp1109I GCAGC 1 cut(s) 964
LweI GCATC 5 cut(s) 112, 189, 838, 844, 946
MaeI CTAG 3 cut(s) 516, 762, 836
MaeII ACGT 2 cut(s) 318, 452
MaeIII GTNAC 2 cut(s) 305, 593
MalI GATC 9 cut(s) 371, 382, 623, 660, 731, 797, 810, 933, 937
MboI GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
MboII GAAGA 6 cut(s) 26, 136, 271, 355, 712, 739
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 1 cut(s) 380
MluCI AATT 6 cut(s) 286, 344, 375, 706, 739, 1063
MlyI GAGTC 3 cut(s) 104, 158, 271
MmeI TCCRAC 6 cut(s) 408, 430, 481, 514, 520, 636
MnlI CCTC 4 cut(s) 61, 180, 967, 993
MseI TTAA 4 cut(s) 189, 576, 843, 1038
MslI CAYNNNNRTG 2 cut(s) 722, 750
MspI CCGG 2 cut(s) 213, 1045
MspR9I CCNGG 2 cut(s) 671, 1046
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 671
NciI CCSGG 1 cut(s) 1046
NdeII GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
NlaIII CATG 5 cut(s) 313, 434, 469, 727, 901
NlaIV GGNNCC 2 cut(s) 382, 681
NmuCI GTSAC 1 cut(s) 593
PagI TCATGA 2 cut(s) 430, 723
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PflFI GACNNNGTC 1 cut(s) 437
PflMI CCANNNNNTGG 2 cut(s) 425, 676
PfoI TCCNGGA 1 cut(s) 1044
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 1 cut(s) 979
PleI GAGTC 3 cut(s) 104, 158, 270
PpsI GAGTC 3 cut(s) 104, 158, 270
PpuMI RGGWCCY 1 cut(s) 680
PsiI TTATAA 1 cut(s) 1056
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 669
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 382, 681
PspPI GGNCC 3 cut(s) 7, 542, 680
PspPPI RGGWCCY 1 cut(s) 680
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 1 cut(s) 380
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 2 cut(s) 722, 750
SaqAI TTAA 4 cut(s) 189, 576, 843, 1038
SatI GCNGC 1 cut(s) 978
Sau3AI GATC 9 cut(s) 369, 380, 621, 658, 729, 795, 808, 931, 935
Sau96I GGNCC 3 cut(s) 7, 542, 680
SchI GAGTC 3 cut(s) 104, 158, 271
ScrFI CCNGG 2 cut(s) 671, 1046
SfaNI GCATC 5 cut(s) 112, 189, 838, 844, 946
SfcI CTRYAG 1 cut(s) 969
SinI GGWCC 2 cut(s) 542, 680
SmiMI CAYNNNNRTG 2 cut(s) 722, 750
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 6 cut(s) 286, 344, 375, 706, 739, 1063
SsiI CCGC 1 cut(s) 851
SspMI CTAG 3 cut(s) 516, 762, 836
StyD4I CCNGG 2 cut(s) 669, 1044
StyI CCWWGG 1 cut(s) 625
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 2 cut(s) 321, 455
TaqI TCGA 7 cut(s) 155, 183, 232, 368, 661, 817, 934
TasI AATT 6 cut(s) 286, 344, 375, 706, 739, 1063
TatI WGTACW 1 cut(s) 41
Tru1I TTAA 4 cut(s) 189, 576, 843, 1038
Tru9I TTAA 4 cut(s) 189, 576, 843, 1038
TscAI CASTG 2 cut(s) 273, 602
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 1 cut(s) 977
Tsp45I GTSAC 1 cut(s) 593
TspDTI ATGAA 7 cut(s) 40, 454, 514, 712, 740, 886, 1032
TspRI CASTG 2 cut(s) 273, 602
Tth111I GACNNNGTC 1 cut(s) 437
Van91I CCANNNNNTGG 2 cut(s) 425, 676
VpaK11BI GGWCC 2 cut(s) 542, 680
XapI RAATTY 2 cut(s) 344, 706
XspI CTAG 3 cut(s) 516, 762, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.