Rh7BG077500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0015993
Physical Location & Seq
Reverse (-)
5476449 .. 5483862
7414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG077500.1

Sequence Viewer

Length: 1203 bp
ATGGAGGTCCTTCAGAAAACTTGTCAGTCTATTCTTCTATGTACAGTTTTCATTGTTCTTTGTTTGGAGGCTTCCCCTTGCTTTGCTTTCACTTCGTTCGTGTTTGGGGACTCTCTGGTTGATGCAGGAAACAATGACTACATCTTCACGCTCTCGAAAGCCGACTCTCCTCCTTATGGCATCGATTTTAAGGCTTCAGGTGGACGACCCACCGGTAGGTTCACAAATGGTCGAACCATATCGGACATCATAGGTCAAGAACTTGGAGCCAAATCATTTCCACCACCTTATCTAGCCCCAGACACCCAAGCCAGCTCAATTCTCAGAGGAATTAATTATGCTTCTGGAGCCTCCGGAATATTAGATGAAACAGGGTTTTTGTTTATTGGAAGAGTCCCACTGCGTGAGCAAGTAAATAATTTTGAGCAGAGCAGAAGTTACATGGTAAACGTAATGGGAGAGAGAAGCACAAGAGAATTTCTAAAGAAGGCAATCTTCTCGATCACAATTGGATCCAACGATGTTCTCAACTATGTCCAACCATCCATACCATTTTTTGGTCATGACAAAGTCTCACCCAACACGTTCCAACATTTCATGGTCTCTAACTTGACAATACAACTCAAGCGACTGCACGAGTTGGGAGCTAGAAAGTTCATTGTGGTTGGAGTTGGACCACTGGGTTGCATACCCTTCATTCGTGCCGTTAATCTTTTACCAAGTGGTCACTGCTTTGGTGAAGTGAATGATTTGATCCAAGGCTACAATAAGAAACTCAATGGTTTGTTGGATCAACTGAACCAGGTGTTGGGTCGTGAAGCTATCTTTGTGTATGCAAATTCTTATGACATCTTCATGAAGATCATAGTGAATTATCATCAATATGGTAAAACTAGGGCTAACCTTTTGTTTATTTTTATTATTGTGATCGTTTATTTTGATCTGTTTCGATATGTTTGGTATTTGCTAGGGTTTAAGGATGCGGACGCACCGTGTTGTGGTGGATACTTTCCACCATTTCTTTGCTTCATGGGAAGGAACACAAGCTCTCCCCTGTGTGGTGATCGATCCAAGTATGTGTTTTGGGATGCTTATCATCCAACAGAGGCTGCTAATGTCATCATAGCTAAGAGGTTGCTTGATGGAGATGAAAGTATTAGCTCACCTATTAATATCCGGGAGCTTTATAACTACAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

400

Amino Acids

44.7

Weight (kDa)

7.51

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 379 1.6e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41890
fragaria_vesca FvH4_5g10830
malus_domestica MD06G1120400.v1.1
prunus_persica Prupe.5G130900_v2.0.a1
pyrus_communis pycom06g11170
rosa_chinensis RchiOBHm_Chr7g0187331
rosa_laevigata RLG00000004794
rosa_multiflora Rmu_sc0001758.1_g000012
rosa_roxburghii Rroxscaffold_3G00267600
rosa_rugosa Rorug06G0480600.1
rosa_samantha Rh7AG085100 Rh7BG077500 Rh7CG086100 Rh7DG087200
rosa_wichuraiana Rw7G007480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1188
AccB7I CCANNNNNTGG 2 cut(s) 557, 808
AccIII TCCGGA 1 cut(s) 353
AciI CCGC 1 cut(s) 983
AclWI GGATC 5 cut(s) 507, 520, 748, 798, 1062
AcsI RAATTY 2 cut(s) 476, 838
AcuI CTGAAG 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 404
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 6 cut(s) 176, 216, 557, 808, 998, 1058
AflIII ACRYGT 1 cut(s) 582
AgeI ACCGGT 1 cut(s) 212
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 2 cut(s) 791, 823
AluBI AGCT 7 cut(s) 315, 647, 821, 1047, 1127, 1161, 1183
AluI AGCT 7 cut(s) 315, 647, 821, 1047, 1127, 1161, 1183
Alw26I GTCTC 2 cut(s) 577, 607
AlwI GGATC 5 cut(s) 507, 520, 748, 798, 1062
AlwNI CAGNNNCTG 1 cut(s) 1109
Aor13HI TCCGGA 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 1109
ApoI RAATTY 2 cut(s) 476, 838
AseI ATTAAT 2 cut(s) 333, 1170
AsiGI ACCGGT 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 7, 674
AsuC2I CCSGG 1 cut(s) 1178
AsuHPI GGTGA 4 cut(s) 567, 749, 1073, 1155
AvaII GGWCC 2 cut(s) 7, 674
BamHI GGATCC 1 cut(s) 512
BauI CACGAG 1 cut(s) 635
BbvI GCAGC 1 cut(s) 1096
BccI CCATC 2 cut(s) 550, 1136
BceAI ACGGC 1 cut(s) 689
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 1 cut(s) 998
BcnI CCSGG 1 cut(s) 1178
BcoDI GTCTC 2 cut(s) 577, 607
BfaI CTAG 4 cut(s) 293, 648, 894, 968
BfuI GTATCC 1 cut(s) 998
BisI GCNGC 1 cut(s) 1110
BlsI GCNGC 1 cut(s) 1111
Bme1390I CCNGG 2 cut(s) 803, 1178
Bme18I GGWCC 2 cut(s) 7, 674
BmgT120I GGNCC 2 cut(s) 7, 674
BmiI GGNNCC 3 cut(s) 268, 349, 514
BmrFI CCNGG 2 cut(s) 803, 1178
BmrI ACTGGG 1 cut(s) 689
BmsI GCATC 4 cut(s) 112, 189, 970, 1078
BmuI ACTGGG 1 cut(s) 689
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 1 cut(s) 1178
Bsa29I ATCGAT 2 cut(s) 183, 1066
BsaBI GATNNNNATC 1 cut(s) 1092
BsaI GGTCTC 1 cut(s) 607
BsaJI CCNNGG 1 cut(s) 757
BsaWI WCCGGW 2 cut(s) 212, 353
BsaXI ACNNNNNCTCC 2 cut(s) 1033, 1063
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 216, 557, 808, 998, 1058
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 1092
BseAI TCCGGA 1 cut(s) 353
BseBI CCWGG 1 cut(s) 803
BseCI ATCGAT 2 cut(s) 183, 1066
BseDI CCNNGG 1 cut(s) 757
BseGI GGATG 4 cut(s) 542, 985, 1093, 1096
BseJI GATNNNNATC 1 cut(s) 1092
BseLI CCNNNNNNNGG 6 cut(s) 176, 216, 557, 808, 998, 1058
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 684
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 1096
BsgI GTGCAG 1 cut(s) 617
BshTI ACCGGT 1 cut(s) 212
BshVI ATCGAT 2 cut(s) 183, 1066
BsiSI CCGG 3 cut(s) 213, 354, 1177
BslFI GGGAC 2 cut(s) 122, 380
BslI CCNNNNNNNGG 6 cut(s) 176, 216, 557, 808, 998, 1058
BsmAI GTCTC 2 cut(s) 577, 607
BsmFI GGGAC 2 cut(s) 122, 380
Bso31I GGTCTC 1 cut(s) 607
Bsp13I TCCGGA 1 cut(s) 353
Bsp1407I TGTACA 1 cut(s) 41
Bsp143I GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
BspACI CCGC 1 cut(s) 983
BspCNI CTCAG 1 cut(s) 336
BspDI ATCGAT 2 cut(s) 183, 1066
BspEI TCCGGA 1 cut(s) 353
BspHI TCATGA 2 cut(s) 562, 855
BspLI GGNNCC 3 cut(s) 268, 349, 514
BspPI GGATC 5 cut(s) 507, 520, 748, 798, 1062
BspTNI GGTCTC 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 41
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 757
BssMI GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
BssSI CACGAG 1 cut(s) 635
BssT1I CCWWGG 1 cut(s) 757
Bst2BI CACGAG 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 2 cut(s) 46, 993
Bst6I CTCTTC 1 cut(s) 385
BstAUI TGTACA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 3 cut(s) 323, 1128, 1200
BstF5I GGATG 4 cut(s) 542, 985, 1093, 1096
BstKTI GATC 9 cut(s) 504, 515, 756, 793, 864, 930, 943, 1066, 1070
BstMAI GTCTC 2 cut(s) 577, 607
BstMBI GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 1 cut(s) 803
BstSCI CCNGG 2 cut(s) 801, 1176
BstV1I GCAGC 1 cut(s) 1096
BstX2I RGATCY 1 cut(s) 512
BstYI RGATCY 1 cut(s) 512
Bsu15I ATCGAT 2 cut(s) 183, 1066
BsuI GTATCC 1 cut(s) 998
BsuTUI ATCGAT 2 cut(s) 183, 1066
BtsCI GGATG 4 cut(s) 542, 985, 1093, 1096
BtsI GCAGTG 2 cut(s) 398, 727
BtsIMutI CAGTG 3 cut(s) 398, 677, 727
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 1109
CciI TCATGA 2 cut(s) 562, 855
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 7, 674
ClaI ATCGAT 2 cut(s) 183, 1066
CseI GACGC 1 cut(s) 995
CsiI ACCWGGT 1 cut(s) 801
Csp6I GTAC 1 cut(s) 42
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 5 cut(s) 442, 563, 598, 856, 1030
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 3 cut(s) 323, 1128, 1200
DpnI GATC 9 cut(s) 503, 514, 755, 792, 863, 929, 942, 1065, 1069
DpnII GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
DraIII CACNNNGTG 1 cut(s) 404
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 757
Eco31I GGTCTC 1 cut(s) 607
Eco47I GGWCC 2 cut(s) 7, 674
Eco57I CTGAAG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 757
FaeI CATG 5 cut(s) 445, 566, 601, 859, 1033
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 2 cut(s) 122, 380
FatI CATG 5 cut(s) 441, 562, 597, 855, 1029
Fnu4HI GCNGC 1 cut(s) 1110
FokI GGATG 4 cut(s) 529, 992, 1083, 1100
Fsp4HI GCNGC 1 cut(s) 1110
FspBI CTAG 4 cut(s) 293, 648, 894, 968
GluI GCNGC 1 cut(s) 1110
GsuI CTGGAG 1 cut(s) 366
HapII CCGG 3 cut(s) 213, 354, 1177
HgaI GACGC 1 cut(s) 995
Hin1II CATG 5 cut(s) 445, 566, 601, 859, 1033
HinfI GANTC 3 cut(s) 110, 164, 393
HpaII CCGG 3 cut(s) 213, 354, 1177
HphI GGTGA 4 cut(s) 567, 749, 1073, 1155
Hpy166II GTNNAC 3 cut(s) 203, 222, 448
Hpy188I TCNGA 3 cut(s) 15, 244, 326
Hpy188III TCNNGA 8 cut(s) 154, 257, 345, 354, 499, 563, 815, 856
Hpy8I GTNNAC 3 cut(s) 203, 222, 448
HpyAV CCTTC 4 cut(s) 20, 481, 703, 1029
HpyCH4III ACNGT 2 cut(s) 46, 993
HpyCH4IV ACGT 2 cut(s) 450, 584
HpyCH4V TGCA 4 cut(s) 125, 634, 687, 836
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 3 cut(s) 323, 1128, 1200
HpySE526I ACGT 2 cut(s) 450, 584
Hsp92II CATG 5 cut(s) 445, 566, 601, 859, 1033
Kpn2I TCCGGA 1 cut(s) 353
Kzo9I GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
LmnI GCTCC 4 cut(s) 266, 347, 644, 1180
Lsp1109I GCAGC 1 cut(s) 1096
LweI GCATC 4 cut(s) 112, 189, 970, 1078
MabI ACCWGGT 1 cut(s) 801
MaeI CTAG 4 cut(s) 293, 648, 894, 968
MaeII ACGT 2 cut(s) 450, 584
MaeIII GTNAC 2 cut(s) 437, 725
MalI GATC 9 cut(s) 503, 514, 755, 792, 863, 929, 942, 1065, 1069
MboI GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
MboII GAAGA 6 cut(s) 26, 136, 402, 487, 844, 871
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 512
MluCI AATT 9 cut(s) 318, 330, 334, 418, 476, 507, 838, 871, 1195
MlyI GAGTC 3 cut(s) 104, 158, 402
MmeI TCCRAC 7 cut(s) 540, 562, 613, 646, 652, 768, 1124
MnlI CCTC 6 cut(s) 61, 180, 320, 361, 1099, 1125
MroI TCCGGA 1 cut(s) 353
MseI TTAA 5 cut(s) 189, 333, 708, 975, 1170
MslI CAYNNNNRTG 2 cut(s) 854, 882
MspI CCGG 3 cut(s) 213, 354, 1177
MspR9I CCNGG 2 cut(s) 803, 1178
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 1 cut(s) 347
NciI CCSGG 1 cut(s) 1178
NdeII GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
NlaIII CATG 5 cut(s) 445, 566, 601, 859, 1033
NlaIV GGNNCC 3 cut(s) 268, 349, 514
NmuCI GTSAC 1 cut(s) 725
PagI TCATGA 2 cut(s) 562, 855
PflFI GACNNNGTC 1 cut(s) 569
PflMI CCANNNNNTGG 2 cut(s) 557, 808
PfoI TCCNGGA 1 cut(s) 1176
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 1 cut(s) 1111
PleI GAGTC 3 cut(s) 104, 158, 401
PpsI GAGTC 3 cut(s) 104, 158, 401
PpuMI RGGWCCY 1 cut(s) 7
PshBI ATTAAT 2 cut(s) 333, 1170
PsiI TTATAA 1 cut(s) 1188
Psp5II RGGWCCY 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 3 cut(s) 268, 349, 514
PspPI GGNCC 2 cut(s) 7, 674
PspPPI RGGWCCY 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 1109
PsuI RGATCY 1 cut(s) 512
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 2 cut(s) 854, 882
SaqAI TTAA 5 cut(s) 189, 333, 708, 975, 1170
SatI GCNGC 1 cut(s) 1110
Sau3AI GATC 9 cut(s) 501, 512, 753, 790, 861, 927, 940, 1063, 1067
Sau96I GGNCC 2 cut(s) 7, 674
SchI GAGTC 3 cut(s) 104, 158, 402
ScrFI CCNGG 2 cut(s) 803, 1178
SexAI ACCWGGT 1 cut(s) 801
SfaNI GCATC 4 cut(s) 112, 189, 970, 1078
SinI GGWCC 2 cut(s) 7, 674
SmiMI CAYNNNNRTG 2 cut(s) 854, 882
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 9 cut(s) 318, 330, 334, 418, 476, 507, 838, 871, 1195
SsiI CCGC 1 cut(s) 983
SspI AATATT 1 cut(s) 360
SspMI CTAG 4 cut(s) 293, 648, 894, 968
StyD4I CCNGG 2 cut(s) 801, 1176
StyI CCWWGG 1 cut(s) 757
TaaI ACNGT 2 cut(s) 46, 993
TaiI ACGT 2 cut(s) 453, 587
TaqI TCGA 6 cut(s) 155, 183, 232, 500, 949, 1066
TasI AATT 9 cut(s) 318, 330, 334, 418, 476, 507, 838, 871, 1195
TatI WGTACW 1 cut(s) 41
Tru1I TTAA 5 cut(s) 189, 333, 708, 975, 1170
Tru9I TTAA 5 cut(s) 189, 333, 708, 975, 1170
TscAI CASTG 3 cut(s) 405, 684, 734
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 1 cut(s) 1109
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 9 cut(s) 40, 381, 586, 646, 685, 844, 872, 1018, 1164
TspRI CASTG 3 cut(s) 405, 684, 734
Tth111I GACNNNGTC 1 cut(s) 569
Van91I CCANNNNNTGG 2 cut(s) 557, 808
VpaK11BI GGWCC 2 cut(s) 7, 674
VspI ATTAAT 2 cut(s) 333, 1170
XapI RAATTY 2 cut(s) 476, 838
XspI CTAG 4 cut(s) 293, 648, 894, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.