Rh7CG086100

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0015993
Physical Location & Seq
Forward (+)
6496295 .. 6499112
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG086100.1

Sequence Viewer

Length: 963 bp
ATGGAGGTCCTTCAGAAAACTTGTCAGTCTATTCTTCTATGTACAGTTTTCATTGTTCTTTGTTTGGAGGCTTCCCCTTGCTTTGCTTTCACTTCGTTCGTGTTTGGGGACTCTCTGGTTGATGCAGGAAACAATGACTACATCTTCACGCTCTCGAAAGCCGACTCGCCTCCTTATGGCATCGATTTTAAGGCTTCAGGTGGACGACCCACCGGTAGGTTCACAAATGGTCGAACCATATCGGACATCATAGGTCAAGAACTTGGAGCCAAATCATTTCCACCACCTTATCTAGCCCCAGACACCGAAGCCAGCTCAATTCTCAGAGGAATTAATTATGCTTCTGGAGCCTCCGGAATATTAGATGAAACAGGGTTCTTGTTTATTGGAAGAGTCCCACTGCGTGAGCAAGTAAATAATTTTGAGCAGAGCAGAAGTTACATGGTAAACGTAATGGGAGAGAGAAGCACAAGAGAATTTCTAAAGAAGGCAATCTTCTCGATCACAATTGGATCCAACGATGTTCTCAACTATGTCCAACCATCCATACCATTTTTTGGTCATGACAAAGTCTCACCCAACACGTTCCAACATTTCATGGTCTCTAACTTGACAATACAACTCAAGCGACTGCACGAGTTGGGAGCTAGAAAGTTCATTGTGGTTGGAGTTGGACCACTGGGTTGCATACCCTTCATTCGTGCCGTTAATCTTTTACAAAGTGGTCACTGCTTTGGTGAAGTGAATGATTTGATCCAAGGCTACAATAAGAAACTCAATGGTTTGTTGGATCAACTGAACCAGGTGTTGGGTCCTGAAGCTATCTTTGTGTATGCAAATTCTTATGACATCTTCATGAAGATCATAGTGAATTATCATCAATATGGTAAAACTAGGGCTAACCTTTTGTTTATTTTTATTATTGTGATCGTTTATTTTGATCTGTTTCGATCAATTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

320

Amino Acids

35.57

Weight (kDa)

7.57

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 283 4.6e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41890
fragaria_vesca FvH4_5g10830
malus_domestica MD06G1120400.v1.1
prunus_persica Prupe.5G130900_v2.0.a1
pyrus_communis pycom06g11170
rosa_chinensis RchiOBHm_Chr7g0187331
rosa_laevigata RLG00000004794
rosa_multiflora Rmu_sc0001758.1_g000012
rosa_roxburghii Rroxscaffold_3G00267600
rosa_rugosa Rorug06G0480600.1
rosa_samantha Rh7AG085100 Rh7BG077500 Rh7CG086100 Rh7DG087200
rosa_wichuraiana Rw7G007480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 557, 808
AccIII TCCGGA 1 cut(s) 353
AclWI GGATC 4 cut(s) 507, 520, 748, 798
AcsI RAATTY 2 cut(s) 476, 838
AcuI CTGAAG 2 cut(s) 180, 837
AdeI CACNNNGTG 1 cut(s) 404
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 4 cut(s) 176, 216, 557, 808
AflIII ACRYGT 1 cut(s) 582
AgeI ACCGGT 1 cut(s) 212
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 2 cut(s) 791, 823
AluBI AGCT 3 cut(s) 315, 647, 821
AluI AGCT 3 cut(s) 315, 647, 821
Alw26I GTCTC 2 cut(s) 577, 607
AlwI GGATC 4 cut(s) 507, 520, 748, 798
Aor13HI TCCGGA 1 cut(s) 353
ApoI RAATTY 2 cut(s) 476, 838
AseI ATTAAT 1 cut(s) 333
AsiGI ACCGGT 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 7, 674, 812
AsuHPI GGTGA 2 cut(s) 567, 749
AvaII GGWCC 3 cut(s) 7, 674, 812
BamHI GGATCC 1 cut(s) 512
BauI CACGAG 1 cut(s) 635
BccI CCATC 1 cut(s) 550
BceAI ACGGC 1 cut(s) 689
BcgI CGANNNNNNTGC 3 cut(s) 480, 514, 939
BciT130I CCWGG 1 cut(s) 803
BcoDI GTCTC 2 cut(s) 577, 607
BfaI CTAG 4 cut(s) 293, 648, 894, 961
Bme1390I CCNGG 1 cut(s) 803
Bme18I GGWCC 3 cut(s) 7, 674, 812
BmgT120I GGNCC 3 cut(s) 7, 674, 812
BmiI GGNNCC 4 cut(s) 268, 349, 514, 813
BmrFI CCNGG 1 cut(s) 803
BmrI ACTGGG 1 cut(s) 689
BmsI GCATC 2 cut(s) 112, 189
BmuI ACTGGG 1 cut(s) 689
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 608
Bsa29I ATCGAT 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 607
BsaJI CCNNGG 1 cut(s) 757
BsaWI WCCGGW 2 cut(s) 212, 353
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 216, 557, 808
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 353
BseBI CCWGG 1 cut(s) 803
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 757
BseGI GGATG 1 cut(s) 542
BseLI CCNNNNNNNGG 4 cut(s) 176, 216, 557, 808
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 684
BsgI GTGCAG 1 cut(s) 617
BshTI ACCGGT 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 183
BsiSI CCGG 2 cut(s) 213, 354
BslFI GGGAC 2 cut(s) 122, 380
BslI CCNNNNNNNGG 4 cut(s) 176, 216, 557, 808
BsmAI GTCTC 2 cut(s) 577, 607
BsmFI GGGAC 2 cut(s) 122, 380
Bso31I GGTCTC 1 cut(s) 607
Bsp13I TCCGGA 1 cut(s) 353
Bsp1407I TGTACA 1 cut(s) 41
Bsp143I GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
BspCNI CTCAG 1 cut(s) 336
BspDI ATCGAT 1 cut(s) 183
BspEI TCCGGA 1 cut(s) 353
BspHI TCATGA 2 cut(s) 562, 855
BspLI GGNNCC 4 cut(s) 268, 349, 514, 813
BspPI GGATC 4 cut(s) 507, 520, 748, 798
BspTNI GGTCTC 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 41
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 757
BssMI GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
BssSI CACGAG 1 cut(s) 635
BssT1I CCWWGG 1 cut(s) 757
Bst2BI CACGAG 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 385
BstAUI TGTACA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 542
BstKTI GATC 8 cut(s) 504, 515, 756, 793, 864, 930, 943, 953
BstMAI GTCTC 2 cut(s) 577, 607
BstMBI GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 1 cut(s) 803
BstSCI CCNGG 1 cut(s) 801
BstX2I RGATCY 1 cut(s) 512
BstYI RGATCY 1 cut(s) 512
Bsu15I ATCGAT 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 183
BtsCI GGATG 1 cut(s) 542
BtsI GCAGTG 2 cut(s) 398, 727
BtsIMutI CAGTG 3 cut(s) 398, 677, 727
Cac8I GCNNGC 1 cut(s) 313
CciI TCATGA 2 cut(s) 562, 855
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 7, 674, 812
ClaI ATCGAT 1 cut(s) 183
CsiI ACCWGGT 1 cut(s) 801
Csp6I GTAC 1 cut(s) 42
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 4 cut(s) 442, 563, 598, 856
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 1 cut(s) 323
DpnI GATC 8 cut(s) 503, 514, 755, 792, 863, 929, 942, 952
DpnII GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
DraIII CACNNNGTG 1 cut(s) 404
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 757
Eco31I GGTCTC 1 cut(s) 607
Eco47I GGWCC 3 cut(s) 7, 674, 812
Eco57I CTGAAG 2 cut(s) 180, 837
EcoO109I RGGNCCY 2 cut(s) 7, 812
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 757
FaeI CATG 4 cut(s) 445, 566, 601, 859
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 2 cut(s) 122, 380
FatI CATG 4 cut(s) 441, 562, 597, 855
FokI GGATG 1 cut(s) 529
FspBI CTAG 4 cut(s) 293, 648, 894, 961
GsuI CTGGAG 1 cut(s) 366
HapII CCGG 2 cut(s) 213, 354
Hin1II CATG 4 cut(s) 445, 566, 601, 859
HinfI GANTC 3 cut(s) 110, 164, 393
HpaII CCGG 2 cut(s) 213, 354
HphI GGTGA 2 cut(s) 567, 749
Hpy166II GTNNAC 3 cut(s) 203, 222, 448
Hpy188I TCNGA 3 cut(s) 15, 244, 326
Hpy188III TCNNGA 8 cut(s) 154, 257, 345, 354, 499, 563, 815, 856
Hpy8I GTNNAC 3 cut(s) 203, 222, 448
HpyAV CCTTC 3 cut(s) 20, 481, 703
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 2 cut(s) 450, 584
HpyCH4V TGCA 4 cut(s) 125, 634, 687, 836
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 2 cut(s) 450, 584
Hsp92II CATG 4 cut(s) 445, 566, 601, 859
Kpn2I TCCGGA 1 cut(s) 353
Kzo9I GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
LmnI GCTCC 3 cut(s) 266, 347, 644
LweI GCATC 2 cut(s) 112, 189
MabI ACCWGGT 1 cut(s) 801
MaeI CTAG 4 cut(s) 293, 648, 894, 961
MaeII ACGT 2 cut(s) 450, 584
MaeIII GTNAC 2 cut(s) 437, 725
MalI GATC 8 cut(s) 503, 514, 755, 792, 863, 929, 942, 952
MboI GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
MboII GAAGA 6 cut(s) 26, 136, 402, 487, 844, 871
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 512
MluCI AATT 9 cut(s) 318, 330, 334, 418, 476, 507, 838, 871, 954
MlyI GAGTC 3 cut(s) 104, 158, 402
MmeI TCCRAC 6 cut(s) 540, 562, 613, 646, 652, 768
MnlI CCTC 4 cut(s) 61, 180, 320, 361
MroI TCCGGA 1 cut(s) 353
MseI TTAA 3 cut(s) 189, 333, 708
MslI CAYNNNNRTG 2 cut(s) 854, 882
MspI CCGG 2 cut(s) 213, 354
MspR9I CCNGG 1 cut(s) 803
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeII GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
NlaIII CATG 4 cut(s) 445, 566, 601, 859
NlaIV GGNNCC 4 cut(s) 268, 349, 514, 813
NmuCI GTSAC 1 cut(s) 725
PagI TCATGA 2 cut(s) 562, 855
PflFI GACNNNGTC 1 cut(s) 569
PflMI CCANNNNNTGG 2 cut(s) 557, 808
PinAI ACCGGT 1 cut(s) 212
PleI GAGTC 3 cut(s) 104, 158, 401
PpsI GAGTC 3 cut(s) 104, 158, 401
PpuMI RGGWCCY 2 cut(s) 7, 812
PshBI ATTAAT 1 cut(s) 333
Psp5II RGGWCCY 2 cut(s) 7, 812
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 4 cut(s) 268, 349, 514, 813
PspPI GGNCC 3 cut(s) 7, 674, 812
PspPPI RGGWCCY 2 cut(s) 7, 812
PsuI RGATCY 1 cut(s) 512
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 2 cut(s) 854, 882
SaqAI TTAA 3 cut(s) 189, 333, 708
Sau3AI GATC 8 cut(s) 501, 512, 753, 790, 861, 927, 940, 950
Sau96I GGNCC 3 cut(s) 7, 674, 812
SchI GAGTC 3 cut(s) 104, 158, 402
ScrFI CCNGG 1 cut(s) 803
SexAI ACCWGGT 1 cut(s) 801
SfaNI GCATC 2 cut(s) 112, 189
SinI GGWCC 3 cut(s) 7, 674, 812
SmiMI CAYNNNNRTG 2 cut(s) 854, 882
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 9 cut(s) 318, 330, 334, 418, 476, 507, 838, 871, 954
SspI AATATT 1 cut(s) 360
SspMI CTAG 4 cut(s) 293, 648, 894, 961
StyD4I CCNGG 1 cut(s) 801
StyI CCWWGG 1 cut(s) 757
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 2 cut(s) 453, 587
TaqI TCGA 5 cut(s) 155, 183, 232, 500, 949
TasI AATT 9 cut(s) 318, 330, 334, 418, 476, 507, 838, 871, 954
TatI WGTACW 1 cut(s) 41
Tru1I TTAA 3 cut(s) 189, 333, 708
Tru9I TTAA 3 cut(s) 189, 333, 708
TscAI CASTG 3 cut(s) 405, 684, 734
TseFI GTSAC 1 cut(s) 725
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 7 cut(s) 40, 381, 586, 646, 685, 844, 872
TspRI CASTG 3 cut(s) 405, 684, 734
Tth111I GACNNNGTC 1 cut(s) 569
Van91I CCANNNNNTGG 2 cut(s) 557, 808
VpaK11BI GGWCC 3 cut(s) 7, 674, 812
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 2 cut(s) 476, 838
XspI CTAG 4 cut(s) 293, 648, 894, 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.