Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013973

Physical Location & Seq

Genomic Coordinates Reverse (-)

27274168 .. 27276911

Gene Structure 2744 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1129200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGAGAATTTGGAAGCTGGGTTTTGCTGGGTTTCTTGTTGCTGCCTTTGTGGTGGGGTTTGGTTCAGGGGAGCAGGCCCGCACTGAGAGAATTTCAGGTAGTGCTGGTGATGTCTTGGATGATGACCCAGTGGGAAGGTTGAAAGTTTTCGTTTATGAGCTTCCAAGCAAATACAACAAGAAGATTCTGCAGAAGGATCCCAGATGCCTCAACCACATGTTTGCAGCTGAGATATTTATGCATCGGTTTCTCCTGTCTAGCCCCGTCCGAACCCTTAATCCTGAAGAAGCAGATTGGTTTTATACTCCTGTATACACCACTTGTGACCTGACACCCAATGGCCTTCCGTTGCCTTTCAAGTCACCACGGATGATGAGAAGTGCAATACAACTTATTTCTTCGAATTGGCCATATTGGAACCGGACAGAAGGGGCTGATCATTTTTTCGTAGTGCCTCATGACTTTGGAGCATGTTTTCATTATCTGGAAGAGAAGGCTATTGAAAGAGGAATTCTTCCCATGCTCCAACGCGCAACTTTGGTTCAGACTTTTGGGCAAAAAAATCATGTTTGCTTGAAAGAGGGCTCAATTACAGTTCCTCCTTATGCTCCTCCACAGAAGATGCAAACCCACCTAATTCCTGAGAAAACTCCTCGGTCCATCTTTGTTTACTTCCGAGGATTATTTTATGATGTGGGAAATGATCCCGAAGGTGGTTATTATGCAAGAGGTGCACGAGCAGCAGTGTGGGAGAACTTCAAAGACAATCCGCTTTTTGACATCTCTACCGAGCACCCCACCACATACTATGAGGACATGCAACGAGCAGTCTTTTGTTTGTGCCCTCTCGGCTGGGCTCCCTGGAGTCCAAGATTGGTCGAAGCAGTGATTTTTGGCTGCATTCCTGTTATCATAGCAGACGACATTGTTCTACCCTTTGCCGATGCCATCCCTTGGGAAGAGATTGGCGTGTTTGTTGATGAGAAGGACGTCCCCAACTTGGACACCATACTCACTTCAATCCCACCTGAACTGATATTGAGGAAGCAGAGATTGCTCGCAAACCCTTCAATGAAGCAAGCAATGTTATTCCCACAGCCAGCCGAATCAGGAGATGCTTTCCACCAAGTTCTGAATGGACTCGCGCGCAAGTTGCCGCACAGCCCTGATGTTTTCTTGAAACCAGGTGAGAAGATCCTGAACTGGACAGCAGGGCCGGTGGGCGACCTGAAGCCTTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0010413 GO:0010417 GO:0012505 GO:0015020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016763 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045491 GO:0045492 GO:0047517 GO:0070589 GO:0070592 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0080116 GO:0097708 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

414

Amino Acids

46.74

Weight (kDa)

6.09

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	46 - 341	6.1e-76	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61840
fragaria_vesca	FvH4_5g09690
malus_domestica	MD06G1129200.v1.1 MD14G1146200.v1.1
prunus_persica	Prupe.5G140500_v2.0.a1
pyrus_communis	pycom06g12080 pycom14g12110
rosa_chinensis	RchiOBHm_Chr7g0188551
rosa_laevigata	RLG00000004684
rosa_multiflora	Rmu_sc0001576.1_g000011 Rmu_sc0016427.1_g000011
rosa_roxburghii	Rroxscaffold_3G00266260
rosa_samantha	Rh7AG096100 Rh7BG097900 Rh7CG098900 Rh7DG098100
rosa_wichuraiana	Rw7G008300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	993
AccB7I	CCANNNNNTGG	1 cut(s)	954
AccI	GTMKAC	1 cut(s)	312
AccII	CGCG	3 cut(s)	531, 1145, 1147
AciI	CCGC	3 cut(s)	79, 770, 1157
AclWI	GGATC	4 cut(s)	191, 204, 698, 1189
AcoI	YGGCCR	1 cut(s)	407
AcsI	RAATTY	3 cut(s)	6, 90, 510
AcuI	CTGAAG	1 cut(s)	303
AcyI	GRCGYC	1 cut(s)	990
AfiI	CCNNNNNNNGG	3 cut(s)	713, 954, 1000
AflIII	ACRYGT	1 cut(s)	216
AgsI	TTSAA	8 cut(s)	142, 358, 503, 577, 760, 1020, 1071, 1180
AjnI	CCWGG	2 cut(s)	860, 1183
AloI	GAACNNNNNNTCC	2 cut(s)	262, 294
AluBI	AGCT	3 cut(s)	16, 160, 227
AluI	AGCT	3 cut(s)	16, 160, 227
Alw21I	GWGCWC	2 cut(s)	736, 795
Alw44I	GTGCAC	1 cut(s)	732
AlwI	GGATC	4 cut(s)	191, 204, 698, 1189
AoxI	GGCC	4 cut(s)	75, 340, 407, 1214
ApaLI	GTGCAC	1 cut(s)	732
ApeKI	GCWGC	4 cut(s)	41, 224, 740, 897
ApoI	RAATTY	3 cut(s)	6, 90, 510
Asp700I	GAANNNNTTC	1 cut(s)	146
AspLEI	GCGC	3 cut(s)	533, 1147, 1149
AspS9I	GGNCC	3 cut(s)	76, 657, 1214
AsuHPI	GGTGA	3 cut(s)	119, 354, 1199
AsuII	TTCGAA	1 cut(s)	401
AvaII	GGWCC	1 cut(s)	657
BaeGI	GKGCMC	2 cut(s)	736, 845
BalI	TGGCCA	1 cut(s)	409
BamHI	GGATCC	1 cut(s)	196
BanII	GRGCYC	2 cut(s)	587, 859
BauI	CACGAG	1 cut(s)	735
Bbv12I	GWGCWC	2 cut(s)	736, 795
BbvI	GCAGC	4 cut(s)	28, 236, 752, 884
BccI	CCATC	2 cut(s)	668, 956
BciT130I	CCWGG	2 cut(s)	862, 1185
BclI	TGATCA	1 cut(s)	436
BfaI	CTAG	1 cut(s)	258
BfmI	CTRYAG	1 cut(s)	188
BglI	GCCNNNNNGGC	1 cut(s)	849
BisI	GCNGC	5 cut(s)	42, 225, 741, 898, 1157
BlsI	GCNGC	5 cut(s)	43, 226, 742, 899, 1158
Bme1390I	CCNGG	2 cut(s)	862, 1185
Bme18I	GGWCC	1 cut(s)	657
BmgT120I	GGNCC	3 cut(s)	76, 657, 1214
BmiI	GGNNCC	3 cut(s)	198, 419, 858
BmrFI	CCNGG	2 cut(s)	862, 1185
BmrI	ACTGGG	1 cut(s)	122
BmsI	GCATC	5 cut(s)	194, 250, 612, 934, 1105
BmuI	ACTGGG	1 cut(s)	122
BpmI	CTGGAG	1 cut(s)	883
Bpu14I	TTCGAA	1 cut(s)	401
BsaHI	GRCGYC	1 cut(s)	990
BsaJI	CCNNGG	6 cut(s)	365, 653, 676, 860, 953, 1235
BsaWI	WCCGGW	1 cut(s)	420
BsaXI	ACNNNNNCTCC	2 cut(s)	583, 613
Bsc4I	CCNNNNNNNGG	3 cut(s)	713, 954, 1000
Bse118I	RCCGGY	1 cut(s)	1216
Bse1I	ACTGG	2 cut(s)	128, 1208
Bse3DI	GCAATG	1 cut(s)	1089
BseBI	CCWGG	2 cut(s)	862, 1185
BseDI	CCNNGG	6 cut(s)	365, 653, 676, 860, 953, 1235
BseGI	GGATG	3 cut(s)	124, 375, 948
BseLI	CCNNNNNNNGG	3 cut(s)	713, 954, 1000
BseMI	GCAATG	1 cut(s)	1089
BseMII	CTCAG	3 cut(s)	75, 219, 633
BseNI	ACTGG	2 cut(s)	128, 1208
BsePI	GCGCGC	1 cut(s)	1145
BseRI	GAGGAG	2 cut(s)	600, 642
BseSI	GKGCMC	2 cut(s)	736, 845
BseXI	GCAGC	4 cut(s)	28, 236, 752, 884
BseYI	CCCAGC	3 cut(s)	16, 26, 852
Bsh1236I	CGCG	3 cut(s)	531, 1145, 1147
BshFI	GGCC	4 cut(s)	77, 342, 409, 1216
BsiHKAI	GWGCWC	2 cut(s)	736, 795
BsiSI	CCGG	2 cut(s)	421, 1217
BslFI	GGGAC	1 cut(s)	977
BslI	CCNNNNNNNGG	3 cut(s)	713, 954, 1000
BsmFI	GGGAC	1 cut(s)	977
BsmI	GAATGC	1 cut(s)	900
BsnI	GGCC	4 cut(s)	77, 342, 409, 1216
Bsp119I	TTCGAA	1 cut(s)	401
Bsp1286I	GDGCHC	5 cut(s)	587, 736, 795, 845, 859
Bsp143I	GATC	4 cut(s)	196, 436, 703, 1194
BspACI	CCGC	3 cut(s)	79, 770, 1157
BspANI	GGCC	4 cut(s)	77, 342, 409, 1216
BspCNI	CTCAG	3 cut(s)	76, 220, 634
BspFNI	CGCG	3 cut(s)	531, 1145, 1147
BspHI	TCATGA	1 cut(s)	457
BspLI	GGNNCC	3 cut(s)	198, 419, 858
BspMAI	CTGCAG	1 cut(s)	192
BspPI	GGATC	4 cut(s)	191, 204, 698, 1189
BspT104I	TTCGAA	1 cut(s)	401
BsrDI	GCAATG	1 cut(s)	1089
BsrFI	RCCGGY	1 cut(s)	1216
BsrI	ACTGG	2 cut(s)	128, 1208
BssAI	RCCGGY	1 cut(s)	1216
BssECI	CCNNGG	6 cut(s)	365, 653, 676, 860, 953, 1235
BssHII	GCGCGC	1 cut(s)	1145
BssMI	GATC	4 cut(s)	196, 436, 703, 1194
BssNAI	GTATAC	1 cut(s)	313
BssNI	GRCGYC	1 cut(s)	990
BssSI	CACGAG	1 cut(s)	735
BssT1I	CCWWGG	2 cut(s)	953, 1235
Bst1107I	GTATAC	1 cut(s)	313
Bst2BI	CACGAG	1 cut(s)	735
Bst2UI	CCWGG	2 cut(s)	862, 1185
Bst4CI	ACNGT	1 cut(s)	595
Bst6I	CTCTTC	2 cut(s)	483, 954
BstACI	GRCGYC	1 cut(s)	990
BstAPI	GCANNNNNTGC	2 cut(s)	731, 1054
BstBI	TTCGAA	1 cut(s)	401
BstC8I	GCNNGC	6 cut(s)	75, 79, 1059, 1080, 1101, 1147
BstDEI	CTNAG	3 cut(s)	84, 228, 642
BstDSI	CCRYGG	1 cut(s)	365
BstF5I	GGATG	3 cut(s)	124, 375, 948
BstFNI	CGCG	3 cut(s)	531, 1145, 1147
BstHHI	GCGC	3 cut(s)	533, 1147, 1149
BstKTI	GATC	4 cut(s)	199, 439, 706, 1197
BstMBI	GATC	4 cut(s)	196, 436, 703, 1194
BstMWI	GCNNNNNNNGC	5 cut(s)	731, 740, 849, 1054, 1153
BstNI	CCWGG	2 cut(s)	862, 1185
BstNSI	RCATGY	3 cut(s)	220, 474, 820
BstSCI	CCNGG	2 cut(s)	860, 1183
BstSFI	CTRYAG	1 cut(s)	188
BstSLI	GKGCMC	2 cut(s)	736, 845
BstUI	CGCG	3 cut(s)	531, 1145, 1147
BstV1I	GCAGC	4 cut(s)	28, 236, 752, 884
BstX2I	RGATCY	2 cut(s)	196, 1194
BstYI	RGATCY	2 cut(s)	196, 1194
BstZ17I	GTATAC	1 cut(s)	313
BsuRI	GGCC	4 cut(s)	77, 342, 409, 1216
BtgI	CCRYGG	1 cut(s)	365
BtsCI	GGATG	3 cut(s)	124, 375, 948
BtsI	GCAGTG	2 cut(s)	750, 891
BtsIMutI	CAGTG	4 cut(s)	81, 135, 750, 891
Cac8I	GCNNGC	6 cut(s)	75, 79, 1059, 1080, 1101, 1147
CciI	TCATGA	1 cut(s)	457
CfoI	GCGC	3 cut(s)	533, 1147, 1149
Cfr10I	RCCGGY	1 cut(s)	1216
Cfr13I	GGNCC	3 cut(s)	76, 657, 1214
CsiI	ACCWGGT	1 cut(s)	1183
CviAII	CATG	6 cut(s)	217, 458, 471, 520, 566, 817
DdeI	CTNAG	3 cut(s)	84, 228, 642
DpnI	GATC	4 cut(s)	198, 438, 705, 1196
DpnII	GATC	4 cut(s)	196, 436, 703, 1194
EaeI	YGGCCR	1 cut(s)	407
Eam1104I	CTCTTC	2 cut(s)	483, 954
EarI	CTCTTC	2 cut(s)	483, 954
Eco130I	CCWWGG	2 cut(s)	953, 1235
Eco24I	GRGCYC	2 cut(s)	587, 859
Eco47I	GGWCC	1 cut(s)	657
Eco57I	CTGAAG	1 cut(s)	303
EcoRI	GAATTC	1 cut(s)	510
EcoRII	CCWGG	2 cut(s)	860, 1183
EcoT14I	CCWWGG	2 cut(s)	953, 1235
EcoT22I	ATGCAT	1 cut(s)	243
EcoT38I	GRGCYC	2 cut(s)	587, 859
ErhI	CCWWGG	2 cut(s)	953, 1235
FaeI	CATG	6 cut(s)	220, 461, 474, 523, 569, 820
FaqI	GGGAC	1 cut(s)	977
FatI	CATG	6 cut(s)	216, 457, 470, 519, 565, 816
FauI	CCCGC	1 cut(s)	86
FbaI	TGATCA	1 cut(s)	436
FblI	GTMKAC	1 cut(s)	312
Fnu4HI	GCNGC	5 cut(s)	42, 225, 741, 898, 1157
FokI	GGATG	3 cut(s)	131, 382, 935
FriOI	GRGCYC	2 cut(s)	587, 859
Fsp4HI	GCNGC	5 cut(s)	42, 225, 741, 898, 1157
FspBI	CTAG	1 cut(s)	258
GlaI	GCGC	3 cut(s)	532, 1146, 1148
GluI	GCNGC	5 cut(s)	42, 225, 741, 898, 1157
GsaI	CCCAGC	3 cut(s)	20, 30, 856
GsuI	CTGGAG	1 cut(s)	883
HaeIII	GGCC	4 cut(s)	77, 342, 409, 1216
HapII	CCGG	2 cut(s)	421, 1217
HhaI	GCGC	3 cut(s)	533, 1147, 1149
Hin1I	GRCGYC	1 cut(s)	990
Hin1II	CATG	6 cut(s)	220, 461, 474, 523, 569, 820
Hin6I	GCGC	3 cut(s)	531, 1145, 1147
HinP1I	GCGC	3 cut(s)	531, 1145, 1147
HinfI	GANTC	4 cut(s)	184, 865, 1106, 1140
HpaII	CCGG	2 cut(s)	421, 1217
HphI	GGTGA	3 cut(s)	119, 354, 1199
Hpy166II	GTNNAC	3 cut(s)	313, 670, 734
Hpy188I	TCNGA	4 cut(s)	269, 546, 677, 1134
Hpy188III	TCNNGA	8 cut(s)	281, 458, 485, 641, 707, 1110, 1177, 1198
Hpy8I	GTNNAC	3 cut(s)	313, 670, 734
HpyAV	CCTTC	8 cut(s)	129, 187, 353, 422, 487, 704, 979, 1077
HpyCH4III	ACNGT	1 cut(s)	595
HpyCH4IV	ACGT	1 cut(s)	990
HpyCH4V	TGCA	9 cut(s)	190, 224, 241, 383, 625, 725, 734, 820, 900
HpyF10VI	GCNNNNNNNGC	5 cut(s)	731, 740, 849, 1054, 1153
HpyF3I	CTNAG	3 cut(s)	84, 228, 642
HpySE526I	ACGT	1 cut(s)	990
Hsp92I	GRCGYC	1 cut(s)	990
Hsp92II	CATG	6 cut(s)	220, 461, 474, 523, 569, 820
HspAI	GCGC	3 cut(s)	531, 1145, 1147
Ksp22I	TGATCA	1 cut(s)	436
Kzo9I	GATC	4 cut(s)	196, 436, 703, 1194
LmnI	GCTCC	5 cut(s)	70, 467, 528, 613, 862
Lsp1109I	GCAGC	4 cut(s)	28, 236, 752, 884
LweI	GCATC	5 cut(s)	194, 250, 612, 934, 1105
MabI	ACCWGGT	1 cut(s)	1183
MaeI	CTAG	1 cut(s)	258
MaeII	ACGT	1 cut(s)	990
MaeIII	GTNAC	2 cut(s)	323, 360
MalI	GATC	4 cut(s)	198, 438, 705, 1196
MboI	GATC	4 cut(s)	196, 436, 703, 1194
MboII	GAAGA	8 cut(s)	193, 296, 390, 500, 506, 631, 971, 1204
MflI	RGATCY	2 cut(s)	196, 1194
MhlI	GDGCHC	5 cut(s)	587, 736, 795, 845, 859
MlsI	TGGCCA	1 cut(s)	409
MluCI	AATT	6 cut(s)	6, 90, 403, 510, 588, 636
MluNI	TGGCCA	1 cut(s)	409
MlyI	GAGTC	2 cut(s)	874, 1134
MmeI	TCCRAC	1 cut(s)	550
Mox20I	TGGCCA	1 cut(s)	409
Mph1103I	ATGCAT	1 cut(s)	243
MroXI	GAANNNNTTC	1 cut(s)	146
MscI	TGGCCA	1 cut(s)	409
MseI	TTAA	1 cut(s)	276
Msp20I	TGGCCA	1 cut(s)	409
MspA1I	CMGCKG	1 cut(s)	227
MspI	CCGG	2 cut(s)	421, 1217
MspR9I	CCNGG	2 cut(s)	862, 1185
Mva1269I	GAATGC	1 cut(s)	900
MvaI	CCWGG	2 cut(s)	862, 1185
MvnI	CGCG	3 cut(s)	531, 1145, 1147
MwoI	GCNNNNNNNGC	5 cut(s)	731, 740, 849, 1054, 1153
NdeII	GATC	4 cut(s)	196, 436, 703, 1194
NlaIII	CATG	6 cut(s)	220, 461, 474, 523, 569, 820
NlaIV	GGNNCC	3 cut(s)	198, 419, 858
NmeAIII	GCCGAG	1 cut(s)	828
NmuCI	GTSAC	2 cut(s)	323, 360
NsiI	ATGCAT	1 cut(s)	243
NspI	RCATGY	3 cut(s)	220, 474, 820
NspV	TTCGAA	1 cut(s)	401
PagI	TCATGA	1 cut(s)	457
PauI	GCGCGC	1 cut(s)	1145
PciI	ACATGT	1 cut(s)	216
PctI	GAATGC	1 cut(s)	900
PdmI	GAANNNNTTC	1 cut(s)	146
PfeI	GAWTC	2 cut(s)	184, 1106
PflMI	CCANNNNNTGG	1 cut(s)	954
PkrI	GCNGC	5 cut(s)	43, 226, 742, 899, 1158
PleI	GAGTC	2 cut(s)	873, 1134
PpsI	GAGTC	2 cut(s)	873, 1134
PscI	ACATGT	1 cut(s)	216
Psp6I	CCWGG	2 cut(s)	860, 1183
PspFI	CCCAGC	3 cut(s)	16, 26, 852
PspGI	CCWGG	2 cut(s)	860, 1183
PspN4I	GGNNCC	3 cut(s)	198, 419, 858
PspPI	GGNCC	3 cut(s)	76, 657, 1214
PstI	CTGCAG	1 cut(s)	192
PsuI	RGATCY	2 cut(s)	196, 1194
PteI	GCGCGC	1 cut(s)	1145
PvuII	CAGCTG	1 cut(s)	227
SaqAI	TTAA	1 cut(s)	276
SatI	GCNGC	5 cut(s)	42, 225, 741, 898, 1157
Sau3AI	GATC	4 cut(s)	196, 436, 703, 1194
Sau96I	GGNCC	3 cut(s)	76, 657, 1214
SchI	GAGTC	2 cut(s)	874, 1134
ScrFI	CCNGG	2 cut(s)	862, 1185
SduI	GDGCHC	5 cut(s)	587, 736, 795, 845, 859
SexAI	ACCWGGT	1 cut(s)	1183
SfaNI	GCATC	5 cut(s)	194, 250, 612, 934, 1105
SfcI	CTRYAG	1 cut(s)	188
SfuI	TTCGAA	1 cut(s)	401
SinI	GGWCC	1 cut(s)	657
Sse9I	AATT	6 cut(s)	6, 90, 403, 510, 588, 636
SsiI	CCGC	3 cut(s)	79, 770, 1157
SspMI	CTAG	1 cut(s)	258
StyD4I	CCNGG	2 cut(s)	860, 1183
StyI	CCWWGG	2 cut(s)	953, 1235
TaaI	ACNGT	1 cut(s)	595
TaiI	ACGT	1 cut(s)	993
TaqI	TCGA	2 cut(s)	401, 879
TaqII	GACCGA	1 cut(s)	645
TasI	AATT	6 cut(s)	6, 90, 403, 510, 588, 636
TauI	GCSGC	1 cut(s)	1159
TfiI	GAWTC	2 cut(s)	184, 1106
Tru1I	TTAA	1 cut(s)	276
Tru9I	TTAA	1 cut(s)	276
TscAI	CASTG	4 cut(s)	88, 135, 750, 891
TseFI	GTSAC	2 cut(s)	323, 360
TseI	GCWGC	4 cut(s)	41, 224, 740, 897
Tsp45I	GTSAC	2 cut(s)	323, 360
TspDTI	ATGAA	2 cut(s)	467, 1088
TspGWI	ACGGA	2 cut(s)	336, 382
TspRI	CASTG	4 cut(s)	88, 135, 750, 891
Van91I	CCANNNNNTGG	1 cut(s)	954
VneI	GTGCAC	1 cut(s)	732
VpaK11BI	GGWCC	1 cut(s)	657
XapI	RAATTY	3 cut(s)	6, 90, 510
XceI	RCATGY	3 cut(s)	220, 474, 820
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1133
XmiI	GTMKAC	1 cut(s)	312
XmnI	GAANNNNTTC	1 cut(s)	146
XspI	CTAG	1 cut(s)	258
ZraI	GACGTC	1 cut(s)	991
Zsp2I	ATGCAT	1 cut(s)	243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.