MD06G1129400.v1.1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015624
Physical Location & Seq
Forward (+)
27292415 .. 27294762
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1129400.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGAGTGCAAGGAAACCATGAAACATGAAGCAGAAGAAGCAGTGACATTGACGAGGATCCGTCGTGTGAGAGAATTTGTTGAAGGCAAGGATCCATCCTACAAGGAGGTGGATGATCTCACAATAAGGAGGTTCCTGCGTGCTCGCGATTTGGATATAGAGCAGGCAGCTGCAATGTTCCTCAAGTACATGAAATGGAGACGCGATTTTGTTCCTAAAGGTTCTATTTGTACTTCTGAGATTCCAAATCAAATTGTTCAGAACAAGATGTTTTTACAGGGATCGGACAAGAAAGGATGCCCCATATCAGTTATCCTTGGTTATAGACATTTTCAAGACAGCCTCAAGGAATTCAAGCGTTATGTAGTCTATGGACTCGACAAGATATGTGCAAAGATTCCGCCAGGACAGAAGTTCATTGCCATAGGAGATCTCGAAGGCTGGGGATATTCAAACAGTGACGTCCGAGGTTACCTTGGAGCTCTTTCAATTTTGCAGGACTACTATCCGGAAAGGCTAGGGAAGGTGTACATCGTCCATGCTCCTCTCATATTCATGACGGTTTGGAAAATCGTTTACCCTTTTATCGACAACAAAACTAAGAAGAAGATAGTATTCGTCGAAAACAAAATGCTGAAATCAACTCTGCTCGAAGAGATTGATGAGAGCCAGCTTCCGGAGATATATGGAGGCAAACTGCCTTTGGTTCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

239

Amino Acids

27.63

Weight (kDa)

8.97

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 88 - 231 2.4e-33 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 99 - 231 3.8e-11 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g09680
malus_domestica MD06G1129400.v1.1 MD14G1146600.v1.1
prunus_persica Prupe.5G140600_v2.0.a1
pyrus_communis pycom06g12120 pycom14g12120
rosa_chinensis RchiOBHm_Chr7g0188571
rosa_laevigata RLG00000004683
rosa_multiflora Rmu_sc0001576.1_g000012
rosa_roxburghii Rroxscaffold_3G00266250
rosa_rugosa Rorug06G0491300
rosa_samantha Rh7AG096300 Rh7BG098000 Rh7CG099000 Rh7DG098300
rosa_wichuraiana Rw7G008310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
AccII CGCG 2 cut(s) 147, 204
AccIII TCCGGA 2 cut(s) 508, 676
AciI CCGC 1 cut(s) 401
AclWI GGATC 5 cut(s) 52, 65, 86, 99, 289
AcsI RAATTY 2 cut(s) 74, 350
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 3 cut(s) 188, 232, 530
AfiI CCNNNNNNNGG 1 cut(s) 676
AgsI TTSAA 5 cut(s) 83, 335, 355, 453, 489
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 3 cut(s) 170, 482, 673
AluI AGCT 3 cut(s) 170, 482, 673
Alw21I GWGCWC 2 cut(s) 145, 484
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 5 cut(s) 52, 65, 86, 99, 289
Aor13HI TCCGGA 2 cut(s) 508, 676
ApeKI GCWGC 2 cut(s) 167, 170
ApoI RAATTY 2 cut(s) 74, 350
BamHI GGATCC 2 cut(s) 57, 91
BanII GRGCYC 1 cut(s) 484
Bbv12I GWGCWC 2 cut(s) 145, 484
BbvI GCAGC 2 cut(s) 157, 179
BccI CCATC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 405
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 518, 715
BglII AGATCT 1 cut(s) 430
BisI GCNGC 2 cut(s) 168, 171
BlsI GCNGC 2 cut(s) 169, 172
Bme1390I CCNGG 1 cut(s) 405
BmiI GGNNCC 4 cut(s) 59, 93, 134, 708
BmrFI CCNGG 1 cut(s) 405
BmsI GCATC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 167, 329
BsaHI GRCGYC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 316, 466, 475
BsaWI WCCGGW 2 cut(s) 508, 676
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse3DI GCAATG 2 cut(s) 180, 417
BseAI TCCGGA 2 cut(s) 508, 676
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 3 cut(s) 316, 466, 475
BseGI GGATG 3 cut(s) 95, 118, 302
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 2 cut(s) 180, 417
BseMII CTCAG 1 cut(s) 228
BseRI GAGGAG 1 cut(s) 534
BseXI GCAGC 2 cut(s) 157, 179
BseYI CCCAGC 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 147, 204
BsiHKAI GWGCWC 2 cut(s) 145, 484
BsiSI CCGG 2 cut(s) 509, 677
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
Bsp1286I GDGCHC 2 cut(s) 145, 484
Bsp13I TCCGGA 2 cut(s) 508, 676
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 5 cut(s) 57, 91, 115, 281, 430
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 1 cut(s) 401
BspCNI CTCAG 1 cut(s) 229
BspEI TCCGGA 2 cut(s) 508, 676
BspFNI CGCG 2 cut(s) 147, 204
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 4 cut(s) 59, 93, 134, 708
BspPI GGATC 5 cut(s) 52, 65, 86, 99, 289
BsrDI GCAATG 2 cut(s) 180, 417
BsrGI TGTACA 1 cut(s) 528
BssECI CCNNGG 3 cut(s) 316, 466, 475
BssMI GATC 5 cut(s) 57, 91, 115, 281, 430
BssNI GRCGYC 1 cut(s) 462
BssT1I CCWWGG 2 cut(s) 316, 475
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 458, 562
Bst6I CTCTTC 1 cut(s) 648
BstACI GRCGYC 1 cut(s) 462
BstAUI TGTACA 1 cut(s) 528
BstC8I GCNNGC 4 cut(s) 141, 145, 165, 671
BstDEI CTNAG 2 cut(s) 237, 600
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 3 cut(s) 95, 118, 302
BstFNI CGCG 2 cut(s) 147, 204
BstKTI GATC 5 cut(s) 60, 94, 118, 284, 433
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 5 cut(s) 57, 91, 115, 281, 430
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 1 cut(s) 405
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 403
BstUI CGCG 2 cut(s) 147, 204
BstV1I GCAGC 2 cut(s) 157, 179
BstX2I RGATCY 3 cut(s) 57, 91, 430
BstYI RGATCY 3 cut(s) 57, 91, 430
BtsCI GGATG 3 cut(s) 95, 118, 302
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 463
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 4 cut(s) 141, 145, 165, 671
CciI TCATGA 1 cut(s) 555
CseI GACGC 1 cut(s) 210
Csp6I GTAC 3 cut(s) 187, 231, 529
CviAII CATG 5 cut(s) 19, 26, 190, 539, 556
CviJI RGCY 7 cut(s) 170, 342, 441, 482, 517, 669, 673
CviKI_1 RGCY 7 cut(s) 170, 342, 441, 482, 517, 669, 673
CviQI GTAC 3 cut(s) 187, 231, 529
DdeI CTNAG 2 cut(s) 237, 600
DpnI GATC 5 cut(s) 59, 93, 117, 283, 432
DpnII GATC 5 cut(s) 57, 91, 115, 281, 430
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
EciI GGCGGA 1 cut(s) 390
Ecl136II GAGCTC 1 cut(s) 482
Eco130I CCWWGG 2 cut(s) 316, 475
Eco24I GRGCYC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 482
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 482
EcoO65I GGTNACC 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 316, 475
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 2 cut(s) 316, 475
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 5 cut(s) 22, 29, 193, 542, 559
FatI CATG 5 cut(s) 18, 25, 189, 538, 555
Fnu4HI GCNGC 2 cut(s) 168, 171
FokI GGATG 3 cut(s) 82, 125, 309
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 2 cut(s) 168, 171
FspBI CTAG 2 cut(s) 518, 715
GluI GCNGC 2 cut(s) 168, 171
GsaI CCCAGC 1 cut(s) 445
HapII CCGG 2 cut(s) 509, 677
HgaI GACGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 5 cut(s) 22, 29, 193, 542, 559
HinfI GANTC 3 cut(s) 241, 375, 397
HpaII CCGG 2 cut(s) 509, 677
Hpy166II GTNNAC 2 cut(s) 529, 577
Hpy188I TCNGA 4 cut(s) 238, 261, 286, 467
Hpy188III TCNNGA 6 cut(s) 146, 335, 434, 509, 556, 677
Hpy8I GTNNAC 2 cut(s) 529, 577
Hpy99I CGWCG 2 cut(s) 66, 623
HpyAV CCTTC 3 cut(s) 77, 431, 517
HpyCH4III ACNGT 2 cut(s) 458, 562
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 4 cut(s) 9, 173, 392, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 2 cut(s) 237, 600
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 5 cut(s) 22, 29, 193, 542, 559
Kpn2I TCCGGA 2 cut(s) 508, 676
Kzo9I GATC 5 cut(s) 57, 91, 115, 281, 430
LmnI GCTCC 2 cut(s) 479, 547
Lsp1109I GCAGC 2 cut(s) 157, 179
LweI GCATC 1 cut(s) 287
MaeI CTAG 2 cut(s) 518, 715
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 43, 458, 470
MalI GATC 5 cut(s) 59, 93, 117, 283, 432
MboI GATC 5 cut(s) 57, 91, 115, 281, 430
MboII GAAGA 4 cut(s) 47, 616, 619, 665
MflI RGATCY 3 cut(s) 57, 91, 430
MhlI GDGCHC 2 cut(s) 145, 484
MluCI AATT 4 cut(s) 74, 252, 350, 489
MlyI GAGTC 1 cut(s) 369
MnlI CCTC 8 cut(s) 48, 100, 123, 191, 353, 461, 555, 683
MroI TCCGGA 2 cut(s) 508, 676
MslI CAYNNNNRTG 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 509, 677
MspR9I CCNGG 1 cut(s) 405
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 2 cut(s) 147, 204
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 5 cut(s) 57, 91, 115, 281, 430
NlaIII CATG 5 cut(s) 22, 29, 193, 542, 559
NlaIV GGNNCC 4 cut(s) 59, 93, 134, 708
NmuCI GTSAC 2 cut(s) 43, 458
NruI TCGCGA 1 cut(s) 147
PagI TCATGA 1 cut(s) 555
PfeI GAWTC 2 cut(s) 241, 397
PkrI GCNGC 2 cut(s) 169, 172
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 403
PspEI GGTNACC 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 441
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 4 cut(s) 59, 93, 134, 708
PsuI RGATCY 3 cut(s) 57, 91, 430
PvuII CAGCTG 1 cut(s) 170
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 3 cut(s) 188, 232, 530
RsaNI GTAC 3 cut(s) 187, 231, 529
RseI CAYNNNNRTG 1 cut(s) 554
SacI GAGCTC 1 cut(s) 484
SatI GCNGC 2 cut(s) 168, 171
Sau3AI GATC 5 cut(s) 57, 91, 115, 281, 430
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 2 cut(s) 145, 484
SfaNI GCATC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 2 cut(s) 182, 344
SmoI CTYRAG 2 cut(s) 182, 344
Sse9I AATT 4 cut(s) 74, 252, 350, 489
SsiI CCGC 1 cut(s) 401
SspMI CTAG 2 cut(s) 518, 715
SstI GAGCTC 1 cut(s) 484
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 2 cut(s) 316, 475
TaaI ACNGT 2 cut(s) 458, 562
TaiI ACGT 1 cut(s) 465
TaqI TCGA 5 cut(s) 378, 435, 588, 621, 651
TasI AATT 4 cut(s) 74, 252, 350, 489
TatI WGTACW 3 cut(s) 186, 230, 528
TfiI GAWTC 2 cut(s) 241, 397
TscAI CASTG 2 cut(s) 48, 463
TseFI GTSAC 2 cut(s) 43, 458
TseI GCWGC 2 cut(s) 167, 170
Tsp45I GTSAC 2 cut(s) 43, 458
TspDTI ATGAA 5 cut(s) 35, 42, 206, 406, 544
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 2 cut(s) 48, 463
XapI RAATTY 2 cut(s) 74, 350
XspI CTAG 2 cut(s) 518, 715
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.