pycom06g12120

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015624
Physical Location & Seq
Forward (+)
17280569 .. 17284123
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12120.5

Sequence Viewer

Length: 621 bp
ATGAAAAACGAAGTGGATGATCTCACAATAAGGAGGTTCCTGCGTGCTCGCGACTTGGATATAGAGCAGGCATCTGCAATGTTCCTCAAGTACATGAAATGGAGACGTGATTTTGTTCCTAAAGGTTCTGTTACTGCTTCTGAGGTTCCAAACCAAATTGCTCAGAACAAGATGTTTTTACAGGGATCGGACAAGAAAGGATGCCCCATATCAGTTATCCTTGGTTATAGACATTTTCAAGACAGCCTCGAGGAATTCAAGCGTTTTGTAGTCTATGGACTCGACAAGATATGTGCAAAGATTCCGCCAGGACAGAAGTTCATTGCCATAGGAGATCTCGAAGGCTGGGGATATTCAAACAGTGACGTCCGAGGTTACCTTGGAGCTCTTTCAATTTTGCAGGACTACTATCCGGAAAGGCTAGGGAAGATGTACATCGTTCATGCTCCTCTCATATTCATGACAGTTTGGAAAATCGTTTACCCTTTTATCGACAACAAAACTAAGAAGAAGATAGTATTCGTCGAAAACAAAATGCTGAAATCAACTCTGCTCGAAGAGATTGATGAGAGCCAGCTTCCGGAGATATATGGAGGCAAACTGCCTTTGGTTCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

207

Amino Acids

23.76

Weight (kDa)

9.04

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g09680
malus_domestica MD06G1129400.v1.1 MD14G1146600.v1.1
prunus_persica Prupe.5G140600_v2.0.a1
pyrus_communis pycom06g12120 pycom14g12120
rosa_chinensis RchiOBHm_Chr7g0188571
rosa_laevigata RLG00000004683
rosa_multiflora Rmu_sc0001576.1_g000012
rosa_roxburghii Rroxscaffold_3G00266250
rosa_rugosa Rorug06G0491300
rosa_samantha Rh7AG096300 Rh7BG098000 Rh7CG099000 Rh7DG098300
rosa_wichuraiana Rw7G008310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 369
AbsI CCTCGAGG 1 cut(s) 248
AccII CGCG 1 cut(s) 51
AccIII TCCGGA 2 cut(s) 412, 580
AciI CCGC 1 cut(s) 305
AclWI GGATC 1 cut(s) 193
AcsI RAATTY 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 2 cut(s) 92, 434
AfiI CCNNNNNNNGG 1 cut(s) 580
AgsI TTSAA 4 cut(s) 239, 259, 357, 393
AjiI CACGTC 1 cut(s) 107
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 2 cut(s) 386, 577
AluI AGCT 2 cut(s) 386, 577
Alw21I GWGCWC 2 cut(s) 49, 388
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 1 cut(s) 193
Ama87I CYCGRG 1 cut(s) 248
Aor13HI TCCGGA 2 cut(s) 412, 580
ApoI RAATTY 1 cut(s) 254
AvaI CYCGRG 1 cut(s) 248
BanII GRGCYC 1 cut(s) 388
Bbv12I GWGCWC 2 cut(s) 49, 388
BciT130I CCWGG 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 2 cut(s) 422, 619
BglII AGATCT 1 cut(s) 334
Bme1390I CCNGG 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 107
BmiI GGNNCC 3 cut(s) 38, 147, 612
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 2 cut(s) 80, 191
BpuEI CTTGAG 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 3 cut(s) 220, 370, 379
BsaWI WCCGGW 2 cut(s) 412, 580
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse3DI GCAATG 2 cut(s) 84, 321
Bse8I GATNNNNATC 1 cut(s) 434
BseAI TCCGGA 2 cut(s) 412, 580
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 3 cut(s) 220, 370, 379
BseGI GGATG 2 cut(s) 22, 206
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMI GCAATG 2 cut(s) 84, 321
BseMII CTCAG 2 cut(s) 132, 176
BseRI GAGGAG 1 cut(s) 438
BseYI CCCAGC 1 cut(s) 345
Bsh1236I CGCG 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 49, 388
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 413, 581
BslI CCNNNNNNNGG 1 cut(s) 580
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 49, 388
Bsp13I TCCGGA 2 cut(s) 412, 580
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 3 cut(s) 19, 185, 334
Bsp68I TCGCGA 1 cut(s) 51
BspACI CCGC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 133, 175
BspEI TCCGGA 2 cut(s) 412, 580
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 3 cut(s) 38, 147, 612
BspPI GGATC 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 84, 321
BsrGI TGTACA 1 cut(s) 432
BssECI CCNNGG 3 cut(s) 220, 370, 379
BssMI GATC 3 cut(s) 19, 185, 334
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 2 cut(s) 220, 379
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 362, 466
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 1 cut(s) 366
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 4 cut(s) 45, 49, 69, 575
BstDEI CTNAG 3 cut(s) 141, 162, 504
BstEII GGTNACC 1 cut(s) 374
BstF5I GGATG 2 cut(s) 22, 206
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 3 cut(s) 22, 188, 337
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 3 cut(s) 19, 185, 334
BstNI CCWGG 1 cut(s) 309
BstPI GGTNACC 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 307
BstUI CGCG 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BtrI CACGTC 1 cut(s) 107
BtsCI GGATG 2 cut(s) 22, 206
BtsIMutI CAGTG 1 cut(s) 367
BtuMI TCGCGA 1 cut(s) 51
Cac8I GCNNGC 4 cut(s) 45, 49, 69, 575
CciI TCATGA 1 cut(s) 459
Csp6I GTAC 2 cut(s) 91, 433
CviAII CATG 3 cut(s) 94, 443, 460
CviJI RGCY 6 cut(s) 246, 345, 386, 421, 573, 577
CviKI_1 RGCY 6 cut(s) 246, 345, 386, 421, 573, 577
CviQI GTAC 2 cut(s) 91, 433
DdeI CTNAG 3 cut(s) 141, 162, 504
DpnI GATC 3 cut(s) 21, 187, 336
DpnII GATC 3 cut(s) 19, 185, 334
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 220, 379
Eco24I GRGCYC 1 cut(s) 388
Eco53kI GAGCTC 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 248
Eco91I GGTNACC 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 220, 379
EcoT38I GRGCYC 1 cut(s) 388
ErhI CCWWGG 2 cut(s) 220, 379
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 3 cut(s) 97, 446, 463
FatI CATG 3 cut(s) 93, 442, 459
FokI GGATG 2 cut(s) 29, 213
FriOI GRGCYC 1 cut(s) 388
FspBI CTAG 2 cut(s) 422, 619
GsaI CCCAGC 1 cut(s) 349
HapII CCGG 2 cut(s) 413, 581
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 3 cut(s) 97, 446, 463
HinfI GANTC 2 cut(s) 279, 301
HpaII CCGG 2 cut(s) 413, 581
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 4 cut(s) 142, 165, 190, 371
Hpy188III TCNNGA 6 cut(s) 50, 239, 338, 413, 460, 581
Hpy8I GTNNAC 1 cut(s) 481
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 2 cut(s) 362, 466
HpyCH4IV ACGT 2 cut(s) 106, 366
HpyCH4V TGCA 3 cut(s) 77, 296, 400
HpyF3I CTNAG 3 cut(s) 141, 162, 504
HpySE526I ACGT 2 cut(s) 106, 366
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 3 cut(s) 97, 446, 463
Kpn2I TCCGGA 2 cut(s) 412, 580
Kzo9I GATC 3 cut(s) 19, 185, 334
LmnI GCTCC 2 cut(s) 383, 451
LweI GCATC 2 cut(s) 80, 191
MaeI CTAG 2 cut(s) 422, 619
MaeII ACGT 2 cut(s) 106, 366
MaeIII GTNAC 3 cut(s) 130, 362, 374
MalI GATC 3 cut(s) 21, 187, 336
MboI GATC 3 cut(s) 19, 185, 334
MboII GAAGA 4 cut(s) 439, 520, 523, 569
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 49, 388
MluCI AATT 3 cut(s) 156, 254, 393
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 8 cut(s) 27, 95, 136, 244, 257, 365, 459, 587
MroI TCCGGA 2 cut(s) 412, 580
MslI CAYNNNNRTG 1 cut(s) 458
MspI CCGG 2 cut(s) 413, 581
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 51
NdeII GATC 3 cut(s) 19, 185, 334
NlaIII CATG 3 cut(s) 97, 446, 463
NlaIV GGNNCC 3 cut(s) 38, 147, 612
NmuCI GTSAC 1 cut(s) 362
NruI TCGCGA 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 248
PagI TCATGA 1 cut(s) 459
PfeI GAWTC 1 cut(s) 301
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 307
PspEI GGTNACC 1 cut(s) 374
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 3 cut(s) 38, 147, 612
PspXI VCTCGAGB 1 cut(s) 248
PsuI RGATCY 1 cut(s) 334
RruI TCGCGA 1 cut(s) 51
RsaI GTAC 2 cut(s) 92, 434
RsaNI GTAC 2 cut(s) 91, 433
RseI CAYNNNNRTG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 388
Sau3AI GATC 3 cut(s) 19, 185, 334
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 2 cut(s) 49, 388
SetI ASST 9 cut(s) 38, 109, 127, 147, 369, 376, 381, 388, 579
SfaNI GCATC 2 cut(s) 80, 191
Sfr274I CTCGAG 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 2 cut(s) 86, 248
SmoI CTYRAG 2 cut(s) 86, 248
Sse9I AATT 3 cut(s) 156, 254, 393
SsiI CCGC 1 cut(s) 305
SspMI CTAG 2 cut(s) 422, 619
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 220, 379
TaaI ACNGT 2 cut(s) 362, 466
TaiI ACGT 2 cut(s) 109, 369
TaqI TCGA 6 cut(s) 249, 282, 339, 492, 525, 555
TasI AATT 3 cut(s) 156, 254, 393
TatI WGTACW 2 cut(s) 90, 432
TfiI GAWTC 1 cut(s) 301
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 362
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 5 cut(s) 17, 110, 310, 431, 448
TspRI CASTG 1 cut(s) 367
XapI RAATTY 1 cut(s) 254
XhoI CTCGAG 1 cut(s) 248
XspI CTAG 2 cut(s) 422, 619
ZraI GACGTC 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.