Prupe.5G140600_v2.0.a1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015624
Physical Location & Seq
Forward (+)
13105967 .. 13109492
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G140600.1

Sequence Viewer

Length: 810 bp
ATGTTGGCGTTGAGTTCAAATGTAAACGATCATTACAAGGAGGAAAGAAGCATGGACAGAAAAGAAGATGTACTGCTTGGAGAAGCTTCACATGCAGATAATAATGATATTCAAGAAAGTAGCAGTGAGTTGGAGGAGCTGCCCAAAATTCGTCTCATGAGAGCATTTGTTGAAGCCCAGGATCCTTCCTCCAAGGAAGTGGATGACCCGACTATCAGAAGGTTCCTGCATGCGCGCGATTTCGACATAAGCCAGGCATCTGCCATGCTCCTCAAGTACTTGAAATGGAGACGAACTTTTGTTCCCAACGGTTTCATTTCTGCTTCAGAGGTGCCAAACCAAATTGCACAGAACAAGATGTTTTTACAGGGATCAGACAAGAAGGGACGTCCAATAGCGGTTGTCTGTGGCAATAGACACTTTCAAGACGGCCTTAAGGAATTCAAGCGTTATGTAGTTTATGGCCTCGACAAGACATGTGCAAGGATCCCACCAGGACAGGAGAAGTTTATTGCCATAGGAGACCTTGAAGGATGGGGATATTCAAACAGTGACATCCGAGGGTACCTTGGAGCTCTATCGATTTTGCAGGATTACTATCCAGAACGACTAGGGAAGCTGTTTATCGTCCATGCGCCGCTGGTATTTATGACAGTTTGGAAAATCGTTTACCCTTTCATTGACAACAAAACTAAGAAGAAGATAGTATTTGTGGAGAACAAAATGCTGAAATCAACCCTGCTGGAAGAGATCGACGAAAGCCAACTTCCGGAGATATACGGAGGCCAACTGCCGTTGGTTCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

270

Amino Acids

30.77

Weight (kDa)

6.25

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g09680
malus_domestica MD06G1129400.v1.1 MD14G1146600.v1.1
prunus_persica Prupe.5G140600_v2.0.a1
pyrus_communis pycom06g12120 pycom14g12120
rosa_chinensis RchiOBHm_Chr7g0188571
rosa_laevigata RLG00000004683
rosa_multiflora Rmu_sc0001576.1_g000012
rosa_roxburghii Rroxscaffold_3G00266250
rosa_rugosa Rorug06G0491300
rosa_samantha Rh7AG096300 Rh7BG098000 Rh7CG099000 Rh7DG098300
rosa_wichuraiana Rw7G008310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 391
Acc65I GGTACC 1 cut(s) 564
AccB1I GGYRCC 2 cut(s) 331, 564
AccII CGCG 2 cut(s) 235, 237
AccIII TCCGGA 1 cut(s) 769
AciI CCGC 2 cut(s) 398, 638
AclWI GGATC 5 cut(s) 176, 189, 379, 481, 494
AcsI RAATTY 2 cut(s) 147, 440
AcuI CTGAAG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 388
AfaI GTAC 3 cut(s) 72, 278, 566
AfiI CCNNNNNNNGG 1 cut(s) 769
AflII CTTAAG 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 476
AgsI TTSAA 8 cut(s) 18, 113, 173, 283, 425, 445, 530, 546
AjnI CCWGG 3 cut(s) 177, 252, 493
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 4 cut(s) 86, 139, 575, 619
AluI AGCT 4 cut(s) 86, 139, 575, 619
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 3 cut(s) 158, 283, 516
AlwI GGATC 5 cut(s) 176, 189, 379, 481, 494
Aor13HI TCCGGA 1 cut(s) 769
AoxI GGCC 3 cut(s) 430, 463, 784
ApeKI GCWGC 1 cut(s) 139
ApoI RAATTY 2 cut(s) 147, 440
Asp718I GGTACC 1 cut(s) 564
AspLEI GCGC 3 cut(s) 235, 237, 637
BamHI GGATCC 2 cut(s) 181, 486
BanI GGYRCC 2 cut(s) 331, 564
BanII GRGCYC 1 cut(s) 577
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 1 cut(s) 126
BccI CCATC 1 cut(s) 528
BceAI ACGGC 2 cut(s) 445, 778
BciT130I CCWGG 3 cut(s) 179, 254, 495
BcoDI GTCTC 3 cut(s) 158, 283, 516
BfaI CTAG 2 cut(s) 611, 808
BfrI CTTAAG 1 cut(s) 434
BisI GCNGC 2 cut(s) 140, 638
BlsI GCNGC 2 cut(s) 141, 639
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 3 cut(s) 179, 254, 495
BmiI GGNNCC 6 cut(s) 183, 224, 333, 488, 566, 801
BmrFI CCNGG 3 cut(s) 179, 254, 495
BmsI GCATC 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 581
BsaBI GATNNNNATC 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 388
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 4 cut(s) 177, 192, 559, 568
BsaWI WCCGGW 1 cut(s) 769
Bsc4I CCNNNNNNNGG 1 cut(s) 769
Bse8I GATNNNNATC 1 cut(s) 597
BseAI TCCGGA 1 cut(s) 769
BseBI CCWGG 3 cut(s) 179, 254, 495
BseCI ATCGAT 1 cut(s) 581
BseDI CCNNGG 4 cut(s) 177, 192, 559, 568
BseGI GGATG 3 cut(s) 208, 539, 555
BseJI GATNNNNATC 1 cut(s) 597
BseLI CCNNNNNNNGG 1 cut(s) 769
BsePI GCGCGC 1 cut(s) 233
BseRI GAGGAG 2 cut(s) 149, 260
BseXI GCAGC 1 cut(s) 126
Bsh1236I CGCG 2 cut(s) 235, 237
BshFI GGCC 3 cut(s) 432, 465, 786
BshNI GGYRCC 2 cut(s) 331, 564
BshVI ATCGAT 1 cut(s) 581
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 1 cut(s) 770
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 1 cut(s) 769
BsmAI GTCTC 3 cut(s) 158, 283, 516
BsmBI CGTCTC 2 cut(s) 158, 283
BsmFI GGGAC 1 cut(s) 399
BsnI GGCC 3 cut(s) 432, 465, 786
Bso31I GGTCTC 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 577
Bsp13I TCCGGA 1 cut(s) 769
Bsp143I GATC 5 cut(s) 28, 181, 371, 486, 750
BspACI CCGC 2 cut(s) 398, 638
BspANI GGCC 3 cut(s) 432, 465, 786
BspDI ATCGAT 1 cut(s) 581
BspEI TCCGGA 1 cut(s) 769
BspFNI CGCG 2 cut(s) 235, 237
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 6 cut(s) 183, 224, 333, 488, 566, 801
BspPI GGATC 5 cut(s) 176, 189, 379, 481, 494
BspT107I GGYRCC 2 cut(s) 331, 564
BspTI CTTAAG 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 516
BssECI CCNNGG 4 cut(s) 177, 192, 559, 568
BssHII GCGCGC 1 cut(s) 233
BssMI GATC 5 cut(s) 28, 181, 371, 486, 750
BssNI GRCGYC 1 cut(s) 388
BssT1I CCWWGG 2 cut(s) 192, 568
Bst2UI CCWGG 3 cut(s) 179, 254, 495
Bst4CI ACNGT 3 cut(s) 311, 551, 655
Bst6I CTCTTC 1 cut(s) 741
BstACI GRCGYC 1 cut(s) 388
BstAFI CTTAAG 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 231, 235
BstDEI CTNAG 1 cut(s) 693
BstF5I GGATG 3 cut(s) 208, 539, 555
BstFNI CGCG 2 cut(s) 235, 237
BstHHI GCGC 3 cut(s) 235, 237, 637
BstKTI GATC 5 cut(s) 31, 184, 374, 489, 753
BstMAI GTCTC 3 cut(s) 158, 283, 516
BstMBI GATC 5 cut(s) 28, 181, 371, 486, 750
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 3 cut(s) 179, 254, 495
BstNSI RCATGY 3 cut(s) 95, 233, 480
BstSCI CCNGG 3 cut(s) 177, 252, 493
BstUI CGCG 2 cut(s) 235, 237
BstV1I GCAGC 1 cut(s) 126
BstX2I RGATCY 2 cut(s) 181, 486
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 2 cut(s) 181, 486
Bsu15I ATCGAT 1 cut(s) 581
BsuRI GGCC 3 cut(s) 432, 465, 786
BsuTUI ATCGAT 1 cut(s) 581
BtsCI GGATG 3 cut(s) 208, 539, 555
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 2 cut(s) 130, 556
Cac8I GCNNGC 2 cut(s) 231, 235
CciI TCATGA 1 cut(s) 156
CfoI GCGC 3 cut(s) 235, 237, 637
ClaI ATCGAT 1 cut(s) 581
Csp6I GTAC 3 cut(s) 71, 277, 565
CviAII CATG 7 cut(s) 52, 92, 157, 230, 265, 477, 632
CviQI GTAC 3 cut(s) 71, 277, 565
DdeI CTNAG 1 cut(s) 693
DpnI GATC 5 cut(s) 30, 183, 373, 488, 752
DpnII GATC 5 cut(s) 28, 181, 371, 486, 750
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 2 cut(s) 192, 568
Eco24I GRGCYC 1 cut(s) 577
Eco31I GGTCTC 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 3 cut(s) 177, 252, 493
EcoT14I CCWWGG 2 cut(s) 192, 568
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 2 cut(s) 192, 568
Esp3I CGTCTC 2 cut(s) 158, 283
FaeI CATG 7 cut(s) 55, 95, 160, 233, 268, 480, 635
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 399
FatI CATG 7 cut(s) 51, 91, 156, 229, 264, 476, 631
Fnu4HI GCNGC 2 cut(s) 140, 638
FokI GGATG 3 cut(s) 215, 542, 546
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 2 cut(s) 140, 638
FspBI CTAG 2 cut(s) 611, 808
GlaI GCGC 3 cut(s) 234, 236, 636
GluI GCNGC 2 cut(s) 140, 638
HaeIII GGCC 3 cut(s) 432, 465, 786
HapII CCGG 1 cut(s) 770
HhaI GCGC 3 cut(s) 235, 237, 637
Hin1I GRCGYC 1 cut(s) 388
Hin1II CATG 7 cut(s) 55, 95, 160, 233, 268, 480, 635
Hin6I GCGC 3 cut(s) 233, 235, 635
HinP1I GCGC 3 cut(s) 233, 235, 635
HindIII AAGCTT 1 cut(s) 84
HpaII CCGG 1 cut(s) 770
Hpy166II GTNNAC 2 cut(s) 25, 670
Hpy188I TCNGA 4 cut(s) 218, 328, 376, 560
Hpy188III TCNNGA 5 cut(s) 113, 157, 425, 602, 770
Hpy8I GTNNAC 2 cut(s) 25, 670
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 4 cut(s) 195, 213, 376, 524
HpyCH4III ACNGT 3 cut(s) 311, 551, 655
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 5 cut(s) 95, 229, 347, 482, 589
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 1 cut(s) 693
HpySE526I ACGT 1 cut(s) 388
Hsp92I GRCGYC 1 cut(s) 388
Hsp92II CATG 7 cut(s) 55, 95, 160, 233, 268, 480, 635
HspAI GCGC 3 cut(s) 233, 235, 635
Kpn2I TCCGGA 1 cut(s) 769
KpnI GGTACC 1 cut(s) 568
Kzo9I GATC 5 cut(s) 28, 181, 371, 486, 750
LmnI GCTCC 3 cut(s) 136, 273, 572
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 1 cut(s) 266
MaeI CTAG 2 cut(s) 611, 808
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 1 cut(s) 551
MalI GATC 5 cut(s) 30, 183, 373, 488, 752
MboI GATC 5 cut(s) 28, 181, 371, 486, 750
MboII GAAGA 4 cut(s) 77, 709, 712, 758
MflI RGATCY 2 cut(s) 181, 486
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 3 cut(s) 147, 342, 440
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 8 cut(s) 34, 127, 199, 281, 322, 476, 554, 776
MroI TCCGGA 1 cut(s) 769
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 640
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 1 cut(s) 770
MspR9I CCNGG 3 cut(s) 179, 254, 495
MvaI CCWGG 3 cut(s) 179, 254, 495
MvnI CGCG 2 cut(s) 235, 237
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeII GATC 5 cut(s) 28, 181, 371, 486, 750
NlaIII CATG 7 cut(s) 55, 95, 160, 233, 268, 480, 635
NlaIV GGNNCC 6 cut(s) 183, 224, 333, 488, 566, 801
NmuCI GTSAC 1 cut(s) 551
NspI RCATGY 3 cut(s) 95, 233, 480
PaeI GCATGC 1 cut(s) 233
PagI TCATGA 1 cut(s) 156
PauI GCGCGC 1 cut(s) 233
PciI ACATGT 1 cut(s) 476
PkrI GCNGC 2 cut(s) 141, 639
PscI ACATGT 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 577
Psp6I CCWGG 3 cut(s) 177, 252, 493
PspGI CCWGG 3 cut(s) 177, 252, 493
PspN4I GGNNCC 6 cut(s) 183, 224, 333, 488, 566, 801
PsuI RGATCY 2 cut(s) 181, 486
PteI GCGCGC 1 cut(s) 233
RsaI GTAC 3 cut(s) 72, 278, 566
RsaNI GTAC 3 cut(s) 71, 277, 565
SacI GAGCTC 1 cut(s) 577
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 2 cut(s) 140, 638
Sau3AI GATC 5 cut(s) 28, 181, 371, 486, 750
ScaI AGTACT 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 179, 254, 495
SduI GDGCHC 1 cut(s) 577
SetI ASST 9 cut(s) 88, 141, 224, 333, 391, 528, 570, 577, 621
SfaNI GCATC 1 cut(s) 266
SmlI CTYRAG 2 cut(s) 272, 434
SmoI CTYRAG 2 cut(s) 272, 434
SphI GCATGC 1 cut(s) 233
Sse9I AATT 3 cut(s) 147, 342, 440
SsiI CCGC 2 cut(s) 398, 638
SspMI CTAG 2 cut(s) 611, 808
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 3 cut(s) 177, 252, 493
StyI CCWWGG 2 cut(s) 192, 568
TaaI ACNGT 3 cut(s) 311, 551, 655
TaiI ACGT 1 cut(s) 391
TaqI TCGA 4 cut(s) 243, 468, 581, 753
TasI AATT 3 cut(s) 147, 342, 440
TatI WGTACW 2 cut(s) 70, 276
TauI GCSGC 1 cut(s) 640
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 2 cut(s) 130, 556
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 1 cut(s) 139
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 2 cut(s) 304, 667
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 2 cut(s) 130, 556
Vha464I CTTAAG 1 cut(s) 434
XapI RAATTY 2 cut(s) 147, 440
XceI RCATGY 3 cut(s) 95, 233, 480
XspI CTAG 2 cut(s) 611, 808
ZraI GACGTC 1 cut(s) 389
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.