MD14G1146600.v1.1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015624
Physical Location & Seq
Forward (+)
23923521 .. 23925140
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1146600.v1.1.491

Sequence Viewer

Length: 729 bp
ATGCTCGAGGGCATGCAGGTAGTGTCTGAGATGGAGTGCAAGGAAACCATGAAGGATGAAGCAGAAGAAGCAGGGACACTGATGAGAATTCGTCGTGTGAGAGAATTCGTCGAAGGCAAAGATCCATCCTCCAAGGAAGTGGATGATCTGACGATAAGAAGGTTCCTGCGTGCTCGTGATTTGGATATAGAGCAGGCATCTGCAATGTTCCTCAAGTACTTGAAATGGAGACGCGATTTTGTTCCTAAAGGTTCTATTTCTGCTTCCGAGATTCCAAACCAAATTGCACAGAACACGATGTTTTTACAGGGATCAGACAAGAATGGATACCCCATAGCAGTTATCCTTGGATATAGACACCTTCAAGACAGCCTCAAGGAATTCAAGCGTAAGTTTTGTAGTCTATGGTCTCGACAAGATATGTGCAAAGATTCCGCCAGGACAGGAGAAGTTCATTGCCATAGGATATCTCAAAGGCTGAAGATATTCAAACAGCGACCTCCGAGGTTACCTTGGAGCTCTTTCAATTTTGCAGGGAAGATGTACATCGTCCATGCGCCTCTCATATTCATGAGGGTTTGGAAAATCGTTTACCCTTTTATCGACAACAAAACTAAGAAGATAGTATTCGTCGAAAACAAAATGCTGAAATCAACTCTGCTCGAAGAGATTGATGAGAGCCGGCTTCCCGAGATATACGGAGGCAAACTACCGTTGGTTCCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

243

Amino Acids

28.34

Weight (kDa)

9.44

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 178 - 235 1.8e-12 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g09680
malus_domestica MD06G1129400.v1.1 MD14G1146600.v1.1
prunus_persica Prupe.5G140600_v2.0.a1
pyrus_communis pycom06g12120 pycom14g12120
rosa_chinensis RchiOBHm_Chr7g0188571
rosa_laevigata RLG00000004683
rosa_multiflora Rmu_sc0001576.1_g000012
rosa_roxburghii Rroxscaffold_3G00266250
rosa_rugosa Rorug06G0491300
rosa_samantha Rh7AG096300 Rh7BG098000 Rh7CG099000 Rh7DG098300
rosa_wichuraiana Rw7G008310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccII CGCG 1 cut(s) 234
AciI CCGC 1 cut(s) 435
AclWI GGATC 2 cut(s) 116, 319
AcsI RAATTY 3 cut(s) 87, 104, 380
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 2 cut(s) 218, 545
AgsI TTSAA 5 cut(s) 223, 365, 385, 490, 526
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 1 cut(s) 519
AluI AGCT 1 cut(s) 519
Alw21I GWGCWC 2 cut(s) 175, 521
Alw26I GTCTC 2 cut(s) 223, 414
AlwI GGATC 2 cut(s) 116, 319
Ama87I CYCGRG 2 cut(s) 5, 689
ApoI RAATTY 3 cut(s) 87, 104, 380
Asp700I GAANNNNTTC 1 cut(s) 485
AspLEI GCGC 1 cut(s) 559
AvaI CYCGRG 2 cut(s) 5, 689
BanII GRGCYC 1 cut(s) 521
BauI CACGAG 1 cut(s) 174
Bbv12I GWGCWC 2 cut(s) 175, 521
BccI CCATC 2 cut(s) 25, 133
BciT130I CCWGG 1 cut(s) 439
BciVI GTATCC 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 223, 414
BfaI CTAG 1 cut(s) 727
BfuAI ACCTGC 1 cut(s) 7
BfuI GTATCC 1 cut(s) 320
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 439
BmeT110I CYCGRG 2 cut(s) 5, 689
BmiI GGNNCC 2 cut(s) 164, 720
BmrFI CCNGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 206
BpuEI CTTGAG 2 cut(s) 197, 359
BsaBI GATNNNNATC 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 4 cut(s) 132, 346, 503, 512
Bse118I RCCGGY 1 cut(s) 681
Bse3DI GCAATG 2 cut(s) 210, 454
Bse8I GATNNNNATC 1 cut(s) 545
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 4 cut(s) 132, 346, 503, 512
BseGI GGATG 3 cut(s) 61, 125, 148
BseJI GATNNNNATC 1 cut(s) 545
BseMI GCAATG 2 cut(s) 210, 454
BseMII CTCAG 1 cut(s) 18
Bsh1236I CGCG 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 175, 521
BsiHKCI CYCGRG 2 cut(s) 5, 689
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 1 cut(s) 88
BsmAI GTCTC 2 cut(s) 223, 414
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 88
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 2 cut(s) 5, 689
Bsp1286I GDGCHC 2 cut(s) 175, 521
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 3 cut(s) 121, 145, 311
BspACI CCGC 1 cut(s) 435
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 164, 720
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 2 cut(s) 116, 319
BspTNI GGTCTC 1 cut(s) 414
BsrDI GCAATG 2 cut(s) 210, 454
BsrFI RCCGGY 1 cut(s) 681
BsrGI TGTACA 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 4 cut(s) 132, 346, 503, 512
BssMI GATC 3 cut(s) 121, 145, 311
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 3 cut(s) 132, 346, 512
Bst2BI CACGAG 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 1 cut(s) 714
Bst6I CTCTTC 1 cut(s) 660
BstAUI TGTACA 1 cut(s) 543
BstC8I GCNNGC 4 cut(s) 14, 171, 195, 683
BstDEI CTNAG 2 cut(s) 27, 615
BstEII GGTNACC 1 cut(s) 507
BstF5I GGATG 3 cut(s) 61, 125, 148
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 3 cut(s) 124, 148, 314
BstMAI GTCTC 2 cut(s) 223, 414
BstMBI GATC 3 cut(s) 121, 145, 311
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 1 cut(s) 16
BstPI GGTNACC 1 cut(s) 507
BstSCI CCNGG 1 cut(s) 437
BstUI CGCG 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 121
BsuI GTATCC 1 cut(s) 320
BtsCI GGATG 3 cut(s) 61, 125, 148
BtsIMutI CAGTG 1 cut(s) 77
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 4 cut(s) 14, 171, 195, 683
CciI TCATGA 1 cut(s) 570
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 1 cut(s) 681
CseI GACGC 1 cut(s) 240
Csp6I GTAC 2 cut(s) 217, 544
CviAII CATG 4 cut(s) 13, 49, 554, 571
CviJI RGCY 5 cut(s) 372, 478, 519, 681, 685
CviKI_1 RGCY 5 cut(s) 372, 478, 519, 681, 685
CviQI GTAC 2 cut(s) 217, 544
DdeI CTNAG 2 cut(s) 27, 615
DpnI GATC 3 cut(s) 123, 147, 313
DpnII GATC 3 cut(s) 121, 145, 311
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 1 cut(s) 424
Ecl136II GAGCTC 1 cut(s) 519
Eco130I CCWWGG 3 cut(s) 132, 346, 512
Eco24I GRGCYC 1 cut(s) 521
Eco31I GGTCTC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 500
Eco88I CYCGRG 2 cut(s) 5, 689
Eco91I GGTNACC 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 519
EcoO65I GGTNACC 1 cut(s) 507
EcoRI GAATTC 3 cut(s) 87, 104, 380
EcoRII CCWGG 1 cut(s) 437
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 3 cut(s) 132, 346, 512
EcoT38I GRGCYC 1 cut(s) 521
ErhI CCWWGG 3 cut(s) 132, 346, 512
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 4 cut(s) 16, 52, 557, 574
FaqI GGGAC 1 cut(s) 88
FatI CATG 4 cut(s) 12, 48, 553, 570
FokI GGATG 3 cut(s) 68, 112, 155
FriOI GRGCYC 1 cut(s) 521
FspBI CTAG 1 cut(s) 727
GlaI GCGC 1 cut(s) 558
HapII CCGG 1 cut(s) 682
HgaI GACGC 1 cut(s) 240
HhaI GCGC 1 cut(s) 559
Hin1II CATG 4 cut(s) 16, 52, 557, 574
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HinfI GANTC 2 cut(s) 271, 431
HpaII CCGG 1 cut(s) 682
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 5 cut(s) 28, 150, 268, 316, 504
Hpy188III TCNNGA 5 cut(s) 176, 365, 411, 571, 689
Hpy8I GTNNAC 1 cut(s) 592
Hpy99I CGWCG 3 cut(s) 96, 113, 635
HpyAV CCTTC 4 cut(s) 46, 107, 153, 371
HpyCH4III ACNGT 1 cut(s) 714
HpyCH4V TGCA 6 cut(s) 16, 39, 203, 287, 426, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 2 cut(s) 27, 615
Hsp92II CATG 4 cut(s) 16, 52, 557, 574
HspAI GCGC 1 cut(s) 557
KroI GCCGGC 1 cut(s) 681
KroNI GCCGGC 1 cut(s) 683
Kzo9I GATC 3 cut(s) 121, 145, 311
LmnI GCTCC 1 cut(s) 516
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 727
MaeIII GTNAC 1 cut(s) 507
MalI GATC 3 cut(s) 123, 147, 313
MboI GATC 3 cut(s) 121, 145, 311
MboII GAAGA 5 cut(s) 77, 493, 550, 631, 677
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 2 cut(s) 175, 521
MluCI AATT 5 cut(s) 87, 104, 282, 380, 526
MnlI CCTC 8 cut(s) 139, 221, 383, 498, 510, 567, 570, 695
MroNI GCCGGC 1 cut(s) 681
MroXI GAANNNNTTC 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 569
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 1 cut(s) 439
MvaI CCWGG 1 cut(s) 439
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 683
NdeII GATC 3 cut(s) 121, 145, 311
NgoMIV GCCGGC 1 cut(s) 681
NlaIII CATG 4 cut(s) 16, 52, 557, 574
NlaIV GGNNCC 2 cut(s) 164, 720
NspI RCATGY 1 cut(s) 16
PaeI GCATGC 1 cut(s) 16
PaeR7I CTCGAG 1 cut(s) 5
PagI TCATGA 1 cut(s) 570
PdiI GCCGGC 1 cut(s) 683
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 2 cut(s) 271, 431
Psp124BI GAGCTC 1 cut(s) 521
Psp6I CCWGG 1 cut(s) 437
PspEI GGTNACC 1 cut(s) 507
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 2 cut(s) 164, 720
PspXI VCTCGAGB 1 cut(s) 5
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 218, 545
RsaNI GTAC 2 cut(s) 217, 544
RseI CAYNNNNRTG 1 cut(s) 569
SacI GAGCTC 1 cut(s) 521
Sau3AI GATC 3 cut(s) 121, 145, 311
ScaI AGTACT 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 439
SduI GDGCHC 2 cut(s) 175, 521
SetI ASST 8 cut(s) 21, 164, 253, 363, 502, 509, 514, 521
SfaNI GCATC 1 cut(s) 206
Sfr274I CTCGAG 1 cut(s) 5
SlaI CTCGAG 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 3 cut(s) 5, 212, 374
SmoI CTYRAG 3 cut(s) 5, 212, 374
SphI GCATGC 1 cut(s) 16
Sse9I AATT 5 cut(s) 87, 104, 282, 380, 526
SsiI CCGC 1 cut(s) 435
SspMI CTAG 1 cut(s) 727
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 437
StyI CCWWGG 3 cut(s) 132, 346, 512
TaaI ACNGT 1 cut(s) 714
TaqI TCGA 6 cut(s) 6, 111, 412, 603, 633, 663
TasI AATT 5 cut(s) 87, 104, 282, 380, 526
TatI WGTACW 2 cut(s) 216, 543
TfiI GAWTC 2 cut(s) 271, 431
TscAI CASTG 1 cut(s) 84
TspDTI ATGAA 4 cut(s) 65, 72, 443, 559
TspGWI ACGGA 1 cut(s) 714
TspRI CASTG 1 cut(s) 84
XapI RAATTY 3 cut(s) 87, 104, 380
XceI RCATGY 1 cut(s) 16
XhoI CTCGAG 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 1 cut(s) 727
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.