RchiOBHm_Chr7g0188571

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015624
Physical Location & Seq
Forward (+)
8025392 .. 8028313
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16838

Sequence Viewer

Length: 801 bp
ATGGATTCTGATATAATACCTGAGTTGGAGTCCAAGAAAACCATGAAAGATGAAGCAGAACAAGTGGTACCAAGGGTAAATACAAACGACGAGATGAAAAGCAGCACTCAAGAAATGGAGGAGATGAGAAGAATTCATCTTATGAGAGCATTTGTTGAAGCAAAAGATCCTTTATCCAAGGTAGTGGATGATCTGATGTTAAGAAGGTTCCTACGTGCTCGTGATTTAGATGTAGAGCAGGCATCAGCCATGTTCCTGAAGTACTTGAAATGGAGACAAGCTTTTGCTCCTAATGGTTCAATTTCTGCTTCAGAAGTACCAAATCAAATTGCACAGAACAAGATGTTTCTACAGGGATCAGACAAGAGAGGATGTCCAATAGCAGTTCTGCTAGGTGCTAGACATTTTCAAGTCAAAGGGGGCCTCAAGGAGTTCAAGCGTTATGTAGTCTACGCTTTCGACAAGATATGTGCAAGGATGCCACCAGGACAGGAGAAGTTTATCTTCATCGGTGATCTTGAGGGTTGGGGATATTCAAACAGTGATATCCGAGCATACTTAGGAGCTGTATCCATTTTGCAGGACTACTATCCAGAAAGATTAGGGAAGATGTTCGTTGTCCATGTGCCTTTTTTGTTTACGACAGTTTGGAAAATCATTTGCCCTTTCATTGACAACAAAACTAAGGAGAAGATAGTATTTGTTGAGAACAAAATGCTAAAATCAACTCTGCTTGAAGAGATTGATGAAAGCCAACTTCCTGAAAAATATGGAGGAAAACTGACATTGGTTCCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

266

Amino Acids

30.61

Weight (kDa)

8.27

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 113 - 259 1.6e-36 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 124 - 261 1.4e-08 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g09680
malus_domestica MD06G1129400.v1.1 MD14G1146600.v1.1
prunus_persica Prupe.5G140600_v2.0.a1
pyrus_communis pycom06g12120 pycom14g12120
rosa_chinensis RchiOBHm_Chr7g0188571
rosa_laevigata RLG00000004683
rosa_multiflora Rmu_sc0001576.1_g000012
rosa_roxburghii Rroxscaffold_3G00266250
rosa_rugosa Rorug06G0491300
rosa_samantha Rh7AG096300 Rh7BG098000 Rh7CG099000 Rh7DG098300
rosa_wichuraiana Rw7G008310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 450
AclWI GGATC 2 cut(s) 161, 364
AcsI RAATTY 1 cut(s) 132
AcuI CTGAAG 2 cut(s) 278, 294
AfaI GTAC 3 cut(s) 69, 263, 318
AgsI TTSAA 7 cut(s) 158, 268, 300, 410, 436, 537, 737
AjnI CCWGG 1 cut(s) 484
AluBI AGCT 2 cut(s) 281, 566
AluI AGCT 2 cut(s) 281, 566
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 2 cut(s) 161, 364
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 1 cut(s) 102
ApoI RAATTY 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 611
Asp718I GGTACC 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 524
BanI GGYRCC 1 cut(s) 67
BauI CACGAG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 486
BciVI GTATCC 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 2 cut(s) 392, 399
BfmI CTRYAG 1 cut(s) 350
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 4 cut(s) 69, 209, 422, 792
BmrFI CCNGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 251, 468
BpuEI CTTGAG 3 cut(s) 93, 410, 539
BsaAI YACGTR 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 71, 177
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 71, 177
BseGI GGATG 3 cut(s) 193, 377, 483
BseMII CTCAG 1 cut(s) 12
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 1 cut(s) 114
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 220
BsmAI GTCTC 1 cut(s) 268
BsnI GGCC 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 4 cut(s) 166, 190, 356, 514
BspANI GGCC 1 cut(s) 423
BspCNI CTCAG 1 cut(s) 13
BspLI GGNNCC 4 cut(s) 69, 209, 422, 792
BspPI GGATC 2 cut(s) 161, 364
BspT107I GGYRCC 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 71, 177
BssMI GATC 4 cut(s) 166, 190, 356, 514
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 2 cut(s) 71, 177
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 2 cut(s) 542, 646
Bst6I CTCTTC 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 3 cut(s) 21, 559, 684
BstF5I GGATG 3 cut(s) 193, 377, 483
BstKTI GATC 4 cut(s) 169, 193, 359, 517
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 4 cut(s) 166, 190, 356, 514
BstNI CCWGG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 350
BstV1I GCAGC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 166
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 1 cut(s) 166
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 1 cut(s) 423
BtsCI GGATG 3 cut(s) 193, 377, 483
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 421
Csp6I GTAC 3 cut(s) 68, 262, 317
CviAII CATG 3 cut(s) 43, 250, 623
CviJI RGCY 5 cut(s) 248, 281, 423, 566, 753
CviKI_1 RGCY 5 cut(s) 248, 281, 423, 566, 753
CviQI GTAC 3 cut(s) 68, 262, 317
DdeI CTNAG 3 cut(s) 21, 559, 684
DpnI GATC 4 cut(s) 168, 192, 358, 516
DpnII GATC 4 cut(s) 166, 190, 356, 514
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 2 cut(s) 71, 177
Eco32I GATATC 1 cut(s) 547
Eco57I CTGAAG 2 cut(s) 278, 294
EcoO109I RGGNCCY 1 cut(s) 421
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 484
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 2 cut(s) 71, 177
ErhI CCWWGG 2 cut(s) 71, 177
FaeI CATG 3 cut(s) 46, 253, 626
FaiI YATR 9 cut(s) 14, 44, 143, 251, 444, 469, 556, 624, 771
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FatI CATG 3 cut(s) 42, 249, 622
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 103
FokI GGATG 3 cut(s) 200, 384, 490
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 2 cut(s) 392, 399
GluI GCNGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 423
Hin1II CATG 3 cut(s) 46, 253, 626
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 2 cut(s) 5, 29
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 2 cut(s) 451, 639
Hpy188I TCNGA 5 cut(s) 10, 195, 313, 361, 551
Hpy188III TCNNGA 6 cut(s) 110, 221, 256, 518, 593, 761
Hpy8I GTNNAC 2 cut(s) 451, 639
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 2 cut(s) 542, 646
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 3 cut(s) 332, 473, 580
HpyF3I CTNAG 3 cut(s) 21, 559, 684
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 3 cut(s) 46, 253, 626
KpnI GGTACC 1 cut(s) 71
Kzo9I GATC 4 cut(s) 166, 190, 356, 514
LmnI GCTCC 2 cut(s) 292, 563
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 2 cut(s) 251, 468
MaeI CTAG 2 cut(s) 392, 399
MaeII ACGT 1 cut(s) 214
MalI GATC 4 cut(s) 168, 192, 358, 516
MboI GATC 4 cut(s) 166, 190, 356, 514
MboII GAAGA 5 cut(s) 141, 496, 619, 703, 749
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 3 cut(s) 132, 300, 327
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 5 cut(s) 112, 362, 434, 514, 767
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 486
MvaI CCWGG 1 cut(s) 486
NdeII GATC 4 cut(s) 166, 190, 356, 514
NlaIII CATG 3 cut(s) 46, 253, 626
NlaIV GGNNCC 4 cut(s) 69, 209, 422, 792
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
Ppu21I YACGTR 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 4 cut(s) 69, 209, 422, 792
PspPI GGNCC 1 cut(s) 421
PsrI GAACNNNNNNTAC 2 cut(s) 51, 83
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 3 cut(s) 69, 263, 318
RsaNI GTAC 3 cut(s) 68, 262, 317
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 1 cut(s) 103
Sau3AI GATC 4 cut(s) 166, 190, 356, 514
Sau96I GGNCC 1 cut(s) 421
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 1 cut(s) 220
SetI ASST 7 cut(s) 22, 183, 209, 217, 283, 397, 568
SfaNI GCATC 2 cut(s) 251, 468
SfcI CTRYAG 1 cut(s) 350
SmlI CTYRAG 3 cut(s) 108, 425, 518
SmoI CTYRAG 3 cut(s) 108, 425, 518
Sse9I AATT 3 cut(s) 132, 300, 327
SspMI CTAG 2 cut(s) 392, 399
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 2 cut(s) 71, 177
TaaI ACNGT 2 cut(s) 542, 646
TaiI ACGT 1 cut(s) 217
TaqI TCGA 1 cut(s) 459
TasI AATT 3 cut(s) 132, 300, 327
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 547
TseI GCWGC 1 cut(s) 102
TspDTI ATGAA 7 cut(s) 59, 66, 110, 125, 496, 658, 762
TspRI CASTG 1 cut(s) 547
XapI RAATTY 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 2 cut(s) 392, 399
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.