Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012346

Physical Location & Seq

Genomic Coordinates Forward (+)

30544585 .. 30548593

Gene Structure 4009 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1165300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGCCACTGGATTTGTCGTCGGAGCAAGACACCGACGATGCCGATATCGAGTTCGTTGAGGTTGATCCCACTGGTCGATACGGTCGGTATAAAGAGGTTCTAGGAAAAGGGGCTTTCAAGAAAGTATATCGAGCATTTGATGAATTGGAAGGAATAGAGGTAGCTTGGAATCAGGTTAAGGTGGCGGATCTACTACGCAACTCCGAAGATTTAGAGAGACTGTATTCGGAAGTTCATTTGCTTAAGACTCTAAAGCACAAGAACATAATTAAGTTTTACAATTCGTGGGTCGATGCCAAGCATGAGAACATCAACCTTATTACGGAGATTTTCACTTCGGGAACTTTACGACAGTACCGGAGAAAGCATAAGCACGTTGATTTGAGGGCGTTGAAGAAATGGTCTAGGCAGATCTTAGAAGGCCTTTGGTATCTTCATAATCATGACCCCCCAGTAATCCATCGAGATTTGAAGTGTGACAACATTTTTGTCAATGGGAACCAAGGTGAAGTCAAAATTGGTGACCTAGGACTGGCTGCTATTCTTCGACAGGCTCGTTCAGCTCACAGTGTCATTGGTACTCCCGAGTTTATGGCGCCAGAACTTTACGAAGAGGAATACAATGAGCTTGTAGACATTTATGCCTTTGGCATGTGCGTGCTAGAGTTGGTGACCTTTGAGTATCCATATGTTGAATGTTCAAATGCTGCTCAAATATTCAAGAAAGTGACATCAGGAGTAAAGCCAGCATCGTTGGCAAAAGTGACAGATCCTGGAGTTAAAGCATTCATAGAAAAATGCATTGTGAAAGTTTCAGAACGCTTGCCTGCAAAAGAACTTCTTATGGATCCTTTTCTCCAATCAGATGACGATCGTGAAAGTATAACTCGCTCTCTACGACCCACTCATTATTCAGATGCAGAAGGCAGTTCTGATCAGATTGATATCGAGACAAAGGATATGGACCCTTCTCATGAGACAAATCGGGACTTCAGTGTGCAAGGTCAGAGGAAAGATGACACGATATTTCTGAAATTACGAATAGCAGATTCTACAGGTCATTTTCGAAATATTCACTTTCCATTTGACATTGGGGAAGACACTGCAATTGCTGTTGCTAGTGAAATGGTTCAAGAGTTAGACCTGACAGATCAAGATGTTTCAACAATTGCTGAACTAATCGACTCTGAAATCCATTCCCACATTCCAGATTGGCCGATCGGCGAAAATTCTGGAGATCATATTGATGGTGAGGCCTCAACTCCTTATAGCCATGCTTCTGAGTATAATGAGGATGCTTCCCCCTTGATACACGAGGACCTTCTTTCTAGTAATCTTGCATTAGAAAGATTGCCTTCAGGCCGCAAGTACTGGTGTGAATCACCCAAGGAAGGCGGTGGAAATTCTCCAGGGAAGTATGTCATTACCAATTTGAATTCTCCAGCGGATCCAGTCACTGCAGGAGGTGCTGAAGAAAACGAGAACTCTCCTGGTAGCAGCCCTAGGGAGGAAGATGATGAAAATGGTGCTGGCCCCTCAAATTCACCAAATGGTGGTGTTGAGGAAGATGAGAAATCTCCTGGTGGCCATAGAGAGGAAAATAATCCAAATGGTGATGGAGCCTCTGAATCACCGAGTGGTGATAGTCATGCAGCTACTAATCCACATCTTATTAATGGAGTTCATCCATTTGAATCAGAAGAAGTAAAGATCATTGCCGAGAAACTTGAGAATCTATTGGTAGAACAGAAAAAGGAGTTAGACGAGCTCAAGAGGAAGCATGAATTAGCAATAGCAGACCTATTGAAAGAACTGCCACCAGTTTTCTGTCAAAAAGTTTTAGATATATGTAAGATGAGATTTCCAGACTACAAAATGCAGTATGAGGGAGACAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

633

Amino Acids

70.86

Weight (kDa)

4.8

Isoelectric Point (pI)

47.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	31 - 286	5.6e-50	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	31 - 273	2.2e-35	Protein tyrosine and serine/threonine kinase
OSR1_C	PF12202	344 - 398	7.6e-06	Oxidative-stress-responsive kinase 1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48260 AT3G48260
fragaria_vesca	FvH4_5g06360 FvH4_5g06360
malus_domestica	MD06G1165300.v1.1 MD14G1170800.v1.1
prunus_persica	Prupe.5G164900_v2.0.a1 Prupe.5G164900_v2.0.a1
pyrus_communis	pycom14g14270
rosa_chinensis	RchiOBHm_Chr7g0192481
rosa_laevigata	RLG00000004362
rosa_multiflora	Rmu_sc0003108.1_g000028
rosa_roxburghii	Rroxscaffold_3G00262990
rosa_rugosa	Rorug07G0001700
rosa_samantha	Rh7AG125800 Rh7BG128100 Rh7CG129700 Rh7DG129600
rosa_wichuraiana	Rw7G010830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	595
AccB7I	CCANNNNNTGG	1 cut(s)	1553
AccI	GTMKAC	1 cut(s)	633
AciI	CCGC	4 cut(s)	185, 1363, 1395, 1445
AclWI	GGATC	7 cut(s)	59, 195, 764, 842, 855, 1442, 1455
AcoI	YGGCCR	2 cut(s)	1214, 1585
AcsI	RAATTY	4 cut(s)	1228, 1402, 1435, 1540
AcuI	CTGAAG	3 cut(s)	976, 1341, 1491
AcyI	GRCGYC	1 cut(s)	596
AdeI	CACNNNGTG	1 cut(s)	1637
AfaI	GTAC	3 cut(s)	356, 580, 1370
AfiI	CCNNNNNNNGG	6 cut(s)	322, 532, 1391, 1507, 1553, 1594
AflII	CTTAAG	1 cut(s)	242
AjnI	CCWGG	4 cut(s)	772, 1408, 1489, 1579
AjuI	GAANNNNNNNTTGG	2 cut(s)	501, 533
AluBI	AGCT	6 cut(s)	164, 563, 628, 1655, 1768, 1896
AluI	AGCT	6 cut(s)	164, 563, 628, 1655, 1768, 1896
Alw21I	GWGCWC	1 cut(s)	1770
Alw26I	GTCTC	4 cut(s)	211, 944, 971, 1884
AlwI	GGATC	7 cut(s)	59, 195, 764, 842, 855, 1442, 1455
AlwNI	CAGNNNCTG	2 cut(s)	773, 1457
Ama87I	CYCGRG	1 cut(s)	584
AoxI	GGCC	6 cut(s)	421, 1214, 1254, 1360, 1531, 1585
ApeKI	GCWGC	4 cut(s)	536, 707, 1497, 1652
ApoI	RAATTY	4 cut(s)	1228, 1402, 1435, 1540
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
AseI	ATTAAT	1 cut(s)	1674
Asp700I	GAANNNNTTC	1 cut(s)	1128
AspA2I	CCTAGG	2 cut(s)	526, 1502
AspLEI	GCGC	1 cut(s)	598
AspS9I	GGNCC	3 cut(s)	964, 1318, 1532
AsuHPI	GGTGA	9 cut(s)	518, 533, 682, 1262, 1374, 1536, 1623, 1625, 1652
AsuII	TTCGAA	1 cut(s)	1066
AvaI	CYCGRG	1 cut(s)	584
AvaII	GGWCC	2 cut(s)	964, 1318
AvrII	CCTAGG	2 cut(s)	526, 1502
BaeI	ACNNNNGTAYC	2 cut(s)	338, 371
BalI	TGGCCA	1 cut(s)	1587
BamHI	GGATCC	2 cut(s)	847, 1447
BanI	GGYRCC	1 cut(s)	595
BanII	GRGCYC	1 cut(s)	1770
BauI	CACGAG	1 cut(s)	1313
BbsI	GAAGAC	1 cut(s)	1104
Bbv12I	GWGCWC	1 cut(s)	1770
BbvI	GCAGC	4 cut(s)	523, 694, 1509, 1664
BccI	CCATC	3 cut(s)	468, 1241, 1610
BciT130I	CCWGG	4 cut(s)	774, 1410, 1491, 1581
BciVI	GTATCC	1 cut(s)	693
BclI	TGATCA	1 cut(s)	934
BcoDI	GTCTC	4 cut(s)	211, 944, 971, 1884
BfaI	CTAG	7 cut(s)	101, 405, 527, 662, 1119, 1329, 1503
BfmI	CTRYAG	2 cut(s)	1053, 1458
BfoI	RGCGCY	1 cut(s)	599
BfrI	CTTAAG	1 cut(s)	242
BfuI	GTATCC	1 cut(s)	693
BglII	AGATCT	1 cut(s)	411
BisI	GCNGC	5 cut(s)	537, 708, 1363, 1498, 1653
BlnI	CCTAGG	2 cut(s)	526, 1502
BlsI	GCNGC	5 cut(s)	538, 709, 1364, 1499, 1654
BmcAI	AGTACT	1 cut(s)	1370
Bme1390I	CCNGG	4 cut(s)	774, 1410, 1491, 1581
Bme18I	GGWCC	2 cut(s)	964, 1318
BmeT110I	CYCGRG	1 cut(s)	584
BmgT120I	GGNCC	3 cut(s)	964, 1318, 1532
BmiI	GGNNCC	7 cut(s)	500, 597, 849, 966, 1449, 1534, 1621
BmrFI	CCNGG	4 cut(s)	774, 1410, 1491, 1581
BmrI	ACTGGG	1 cut(s)	446
BmsI	GCATC	5 cut(s)	28, 283, 758, 907, 1285
BmuI	ACTGGG	1 cut(s)	446
BpiI	GAAGAC	1 cut(s)	1104
BpmI	CTGGAG	4 cut(s)	795, 1254, 1392, 1425
Bpu14I	TTCGAA	1 cut(s)	1066
BpuEI	CTTGAG	2 cut(s)	1748, 1754
BsaBI	GATNNNNATC	2 cut(s)	870, 944
BsaHI	GRCGYC	1 cut(s)	596
BsaJI	CCNNGG	5 cut(s)	502, 526, 1386, 1409, 1502
BsaWI	WCCGGW	1 cut(s)	357
Bsc4I	CCNNNNNNNGG	6 cut(s)	322, 532, 1391, 1507, 1553, 1594
Bse1I	ACTGG	7 cut(s)	12, 76, 452, 537, 1376, 1451, 1820
Bse3DI	GCAATG	1 cut(s)	1713
Bse8I	GATNNNNATC	2 cut(s)	870, 944
BseBI	CCWGG	4 cut(s)	774, 1410, 1491, 1581
BseDI	CCNNGG	5 cut(s)	502, 526, 1386, 1409, 1502
BseGI	GGATG	2 cut(s)	1300, 1684
BseJI	GATNNNNATC	2 cut(s)	870, 944
BseLI	CCNNNNNNNGG	6 cut(s)	322, 532, 1391, 1507, 1553, 1594
BseMI	GCAATG	1 cut(s)	1713
BseMII	CTCAG	1 cut(s)	1272
BseNI	ACTGG	7 cut(s)	12, 76, 452, 537, 1376, 1451, 1820
BseXI	GCAGC	4 cut(s)	523, 694, 1509, 1664
Bsh1285I	CGRYCG	3 cut(s)	85, 874, 1221
BshFI	GGCC	6 cut(s)	423, 1216, 1256, 1362, 1533, 1587
BshNI	GGYRCC	1 cut(s)	595
BsiEI	CGRYCG	3 cut(s)	85, 874, 1221
BsiHKAI	GWGCWC	1 cut(s)	1770
BsiHKCI	CYCGRG	1 cut(s)	584
BsiSI	CCGG	1 cut(s)	358
BslFI	GGGAC	1 cut(s)	1001
BslI	CCNNNNNNNGG	6 cut(s)	322, 532, 1391, 1507, 1553, 1594
BsmAI	GTCTC	4 cut(s)	211, 944, 971, 1884
BsmFI	GGGAC	1 cut(s)	1001
BsmI	GAATGC	1 cut(s)	785
BsnI	GGCC	6 cut(s)	423, 1216, 1256, 1362, 1533, 1587
BsoBI	CYCGRG	1 cut(s)	584
Bsp119I	TTCGAA	1 cut(s)	1066
Bsp1286I	GDGCHC	1 cut(s)	1770
BspACI	CCGC	4 cut(s)	185, 1363, 1395, 1445
BspANI	GGCC	6 cut(s)	423, 1216, 1256, 1362, 1533, 1587
BspCNI	CTCAG	1 cut(s)	1273
BspHI	TCATGA	2 cut(s)	442, 973
BspLI	GGNNCC	7 cut(s)	500, 597, 849, 966, 1449, 1534, 1621
BspMAI	CTGCAG	1 cut(s)	1462
BspPI	GGATC	7 cut(s)	59, 195, 764, 842, 855, 1442, 1455
BspT104I	TTCGAA	1 cut(s)	1066
BspT107I	GGYRCC	1 cut(s)	595
BspTI	CTTAAG	1 cut(s)	242
BsrDI	GCAATG	1 cut(s)	1713
BsrI	ACTGG	7 cut(s)	12, 76, 452, 537, 1376, 1451, 1820
BssECI	CCNNGG	5 cut(s)	502, 526, 1386, 1409, 1502
BssNI	GRCGYC	1 cut(s)	596
BssSI	CACGAG	1 cut(s)	1313
BssT1I	CCWWGG	4 cut(s)	502, 526, 1386, 1502
Bst2BI	CACGAG	1 cut(s)	1313
Bst2UI	CCWGG	4 cut(s)	774, 1410, 1491, 1581
Bst4CI	ACNGT	4 cut(s)	83, 222, 354, 569
Bst6I	CTCTTC	1 cut(s)	606
BstACI	GRCGYC	1 cut(s)	596
BstAFI	CTTAAG	1 cut(s)	242
BstAPI	GCANNNNNTGC	1 cut(s)	1466
BstBI	TTCGAA	1 cut(s)	1066
BstC8I	GCNNGC	5 cut(s)	659, 747, 824, 828, 1531
BstDEI	CTNAG	2 cut(s)	415, 1281
BstEII	GGTNACC	2 cut(s)	521, 670
BstF5I	GGATG	2 cut(s)	1300, 1684
BstH2I	RGCGCY	1 cut(s)	599
BstHHI	GCGC	1 cut(s)	598
BstMAI	GTCTC	4 cut(s)	211, 944, 971, 1884
BstMCI	CGRYCG	3 cut(s)	85, 874, 1221
BstMWI	GCNNNNNNNGC	3 cut(s)	560, 755, 1466
BstNI	CCWGG	4 cut(s)	774, 1410, 1491, 1581
BstNSI	RCATGY	1 cut(s)	655
BstPI	GGTNACC	2 cut(s)	521, 670
BstSCI	CCNGG	4 cut(s)	772, 1408, 1489, 1579
BstSFI	CTRYAG	2 cut(s)	1053, 1458
BstV1I	GCAGC	4 cut(s)	523, 694, 1509, 1664
BstV2I	GAAGAC	1 cut(s)	1104
BstX2I	RGATCY	5 cut(s)	187, 411, 769, 847, 1447
BstYI	RGATCY	5 cut(s)	187, 411, 769, 847, 1447
BsuI	GTATCC	1 cut(s)	693
BsuRI	GGCC	6 cut(s)	423, 1216, 1256, 1362, 1533, 1587
BtsCI	GGATG	2 cut(s)	1300, 1684
BtsI	GCAGTG	2 cut(s)	1101, 1455
BtsIMutI	CAGTG	6 cut(s)	5, 69, 574, 1000, 1101, 1455
Cac8I	GCNNGC	5 cut(s)	659, 747, 824, 828, 1531
CaiI	CAGNNNCTG	2 cut(s)	773, 1457
CciI	TCATGA	2 cut(s)	442, 973
CfoI	GCGC	1 cut(s)	598
Cfr13I	GGNCC	3 cut(s)	964, 1318, 1532
Csp6I	GTAC	3 cut(s)	355, 579, 1369
CspCI	CAANNNNNGTGG	1 cut(s)	29
CviAII	CATG	7 cut(s)	302, 443, 652, 974, 1274, 1649, 1781
CviQI	GTAC	3 cut(s)	355, 579, 1369
DdeI	CTNAG	2 cut(s)	415, 1281
DinI	GGCGCC	1 cut(s)	597
DraIII	CACNNNGTG	1 cut(s)	1637
EaeI	YGGCCR	2 cut(s)	1214, 1585
Eam1104I	CTCTTC	1 cut(s)	606
EarI	CTCTTC	1 cut(s)	606
EciI	GGCGGA	1 cut(s)	200
Ecl136II	GAGCTC	1 cut(s)	1768
Eco130I	CCWWGG	4 cut(s)	502, 526, 1386, 1502
Eco147I	AGGCCT	2 cut(s)	423, 1256
Eco24I	GRGCYC	1 cut(s)	1770
Eco32I	GATATC	2 cut(s)	46, 946
Eco47I	GGWCC	2 cut(s)	964, 1318
Eco53kI	GAGCTC	1 cut(s)	1768
Eco57I	CTGAAG	3 cut(s)	976, 1341, 1491
Eco88I	CYCGRG	1 cut(s)	584
Eco91I	GGTNACC	2 cut(s)	521, 670
EcoICRI	GAGCTC	1 cut(s)	1768
EcoO109I	RGGNCCY	1 cut(s)	1318
EcoO65I	GGTNACC	2 cut(s)	521, 670
EcoRI	GAATTC	1 cut(s)	1435
EcoRII	CCWGG	4 cut(s)	772, 1408, 1489, 1579
EcoRV	GATATC	2 cut(s)	46, 946
EcoT14I	CCWWGG	4 cut(s)	502, 526, 1386, 1502
EcoT22I	ATGCAT	1 cut(s)	803
EcoT38I	GRGCYC	1 cut(s)	1770
EgeI	GGCGCC	1 cut(s)	597
EheI	GGCGCC	1 cut(s)	597
ErhI	CCWWGG	4 cut(s)	502, 526, 1386, 1502
FaeI	CATG	7 cut(s)	305, 446, 655, 977, 1277, 1652, 1784
FalI	AAGNNNNNCTT	4 cut(s)	825, 857, 1339, 1371
FaqI	GGGAC	1 cut(s)	1001
FatI	CATG	7 cut(s)	301, 442, 651, 973, 1273, 1648, 1780
FauNDI	CATATG	1 cut(s)	688
FbaI	TGATCA	1 cut(s)	934
FblI	GTMKAC	1 cut(s)	633
Fnu4HI	GCNGC	5 cut(s)	537, 708, 1363, 1498, 1653
FokI	GGATG	2 cut(s)	1307, 1671
FriOI	GRGCYC	1 cut(s)	1770
Fsp4HI	GCNGC	5 cut(s)	537, 708, 1363, 1498, 1653
FspBI	CTAG	7 cut(s)	101, 405, 527, 662, 1119, 1329, 1503
GlaI	GCGC	1 cut(s)	597
GluI	GCNGC	5 cut(s)	537, 708, 1363, 1498, 1653
GsuI	CTGGAG	4 cut(s)	795, 1254, 1392, 1425
HaeII	RGCGCY	1 cut(s)	599
HaeIII	GGCC	6 cut(s)	423, 1216, 1256, 1362, 1533, 1587
HapII	CCGG	1 cut(s)	358
HhaI	GCGC	1 cut(s)	598
Hin1I	GRCGYC	1 cut(s)	596
Hin1II	CATG	7 cut(s)	305, 446, 655, 977, 1277, 1652, 1784
Hin6I	GCGC	1 cut(s)	596
HinP1I	GCGC	1 cut(s)	596
HinfI	GANTC	8 cut(s)	169, 247, 1049, 1184, 1379, 1628, 1694, 1732
HpaII	CCGG	1 cut(s)	358
HphI	GGTGA	9 cut(s)	518, 533, 682, 1262, 1374, 1536, 1623, 1625, 1652
Hpy166II	GTNNAC	1 cut(s)	634
Hpy8I	GTNNAC	1 cut(s)	634
Hpy99I	CGWCG	2 cut(s)	22, 38
HpyAV	CCTTC	7 cut(s)	143, 413, 917, 978, 1331, 1365, 1385
HpyCH4III	ACNGT	4 cut(s)	83, 222, 354, 569
HpyCH4IV	ACGT	1 cut(s)	375
HpyCH4V	TGCA	9 cut(s)	801, 830, 920, 1000, 1106, 1340, 1460, 1652, 1879
HpyF10VI	GCNNNNNNNGC	3 cut(s)	560, 755, 1466
HpyF3I	CTNAG	2 cut(s)	415, 1281
HpySE526I	ACGT	1 cut(s)	375
Hsp92I	GRCGYC	1 cut(s)	596
Hsp92II	CATG	7 cut(s)	305, 446, 655, 977, 1277, 1652, 1784
HspAI	GCGC	1 cut(s)	596
KasI	GGCGCC	1 cut(s)	595
Ksp22I	TGATCA	1 cut(s)	934
LmnI	GCTCC	2 cut(s)	22, 1619
Lsp1109I	GCAGC	4 cut(s)	523, 694, 1509, 1664
LweI	GCATC	5 cut(s)	28, 283, 758, 907, 1285
MaeI	CTAG	7 cut(s)	101, 405, 527, 662, 1119, 1329, 1503
MaeII	ACGT	1 cut(s)	375
MaeIII	GTNAC	6 cut(s)	476, 521, 670, 727, 763, 1453
MfeI	CAATTG	2 cut(s)	1107, 1167
MflI	RGATCY	5 cut(s)	187, 411, 769, 847, 1447
MhlI	GDGCHC	1 cut(s)	1770
MlsI	TGGCCA	1 cut(s)	1587
MluNI	TGGCCA	1 cut(s)	1587
Mly113I	GGCGCC	1 cut(s)	596
MlyI	GAGTC	2 cut(s)	241, 1178
Mox20I	TGGCCA	1 cut(s)	1587
Mph1103I	ATGCAT	1 cut(s)	803
MroXI	GAANNNNTTC	1 cut(s)	1128
MscI	TGGCCA	1 cut(s)	1587
MseI	TTAA	5 cut(s)	177, 243, 270, 780, 1674
MslI	CAYNNNNRTG	3 cut(s)	441, 656, 1245
Msp20I	TGGCCA	1 cut(s)	1587
MspA1I	CMGCKG	1 cut(s)	1445
MspCI	CTTAAG	1 cut(s)	242
MspI	CCGG	1 cut(s)	358
MspR9I	CCNGG	4 cut(s)	774, 1410, 1491, 1581
MunI	CAATTG	2 cut(s)	1107, 1167
Mva1269I	GAATGC	1 cut(s)	785
MvaI	CCWGG	4 cut(s)	774, 1410, 1491, 1581
MwoI	GCNNNNNNNGC	3 cut(s)	560, 755, 1466
NarI	GGCGCC	1 cut(s)	596
NdeI	CATATG	1 cut(s)	688
NlaIII	CATG	7 cut(s)	305, 446, 655, 977, 1277, 1652, 1784
NlaIV	GGNNCC	7 cut(s)	500, 597, 849, 966, 1449, 1534, 1621
NmeAIII	GCCGAG	1 cut(s)	1744
NmuCI	GTSAC	6 cut(s)	476, 521, 670, 727, 763, 1453
NsiI	ATGCAT	1 cut(s)	803
NspI	RCATGY	1 cut(s)	655
NspV	TTCGAA	1 cut(s)	1066
PagI	TCATGA	2 cut(s)	442, 973
PceI	AGGCCT	2 cut(s)	423, 1256
PcsI	WCGNNNNNNNCGW	2 cut(s)	553, 895
PctI	GAATGC	1 cut(s)	785
PdmI	GAANNNNTTC	1 cut(s)	1128
PfeI	GAWTC	6 cut(s)	169, 1049, 1379, 1628, 1694, 1732
PflMI	CCANNNNNTGG	1 cut(s)	1553
PfoI	TCCNGGA	1 cut(s)	772
PkrI	GCNGC	5 cut(s)	538, 709, 1364, 1499, 1654
Ple19I	CGATCG	2 cut(s)	874, 1221
PleI	GAGTC	2 cut(s)	241, 1178
PluTI	GGCGCC	1 cut(s)	599
PpsI	GAGTC	2 cut(s)	241, 1178
PpuMI	RGGWCCY	1 cut(s)	1318
PshBI	ATTAAT	1 cut(s)	1674
Psp124BI	GAGCTC	1 cut(s)	1770
Psp5II	RGGWCCY	1 cut(s)	1318
Psp6I	CCWGG	4 cut(s)	772, 1408, 1489, 1579
PspEI	GGTNACC	2 cut(s)	521, 670
PspGI	CCWGG	4 cut(s)	772, 1408, 1489, 1579
PspN4I	GGNNCC	7 cut(s)	500, 597, 849, 966, 1449, 1534, 1621
PspPI	GGNCC	3 cut(s)	964, 1318, 1532
PspPPI	RGGWCCY	1 cut(s)	1318
PstI	CTGCAG	1 cut(s)	1462
PstNI	CAGNNNCTG	2 cut(s)	773, 1457
PsuI	RGATCY	5 cut(s)	187, 411, 769, 847, 1447
PvuI	CGATCG	2 cut(s)	874, 1221
RsaI	GTAC	3 cut(s)	356, 580, 1370
RsaNI	GTAC	3 cut(s)	355, 579, 1369
RseI	CAYNNNNRTG	3 cut(s)	441, 656, 1245
SacI	GAGCTC	1 cut(s)	1770
SaqAI	TTAA	5 cut(s)	177, 243, 270, 780, 1674
SatI	GCNGC	5 cut(s)	537, 708, 1363, 1498, 1653
Sau96I	GGNCC	3 cut(s)	964, 1318, 1532
ScaI	AGTACT	1 cut(s)	1370
SchI	GAGTC	2 cut(s)	241, 1178
ScrFI	CCNGG	4 cut(s)	774, 1410, 1491, 1581
SduI	GDGCHC	1 cut(s)	1770
SfaNI	GCATC	5 cut(s)	28, 283, 758, 907, 1285
SfcI	CTRYAG	2 cut(s)	1053, 1458
SfoI	GGCGCC	1 cut(s)	597
SfuI	TTCGAA	1 cut(s)	1066
SinI	GGWCC	2 cut(s)	964, 1318
SmiMI	CAYNNNNRTG	3 cut(s)	441, 656, 1245
SmlI	CTYRAG	3 cut(s)	242, 1727, 1769
SmoI	CTYRAG	3 cut(s)	242, 1727, 1769
SseBI	AGGCCT	2 cut(s)	423, 1256
SsiI	CCGC	4 cut(s)	185, 1363, 1395, 1445
SspDI	GGCGCC	1 cut(s)	595
SspI	AATATT	2 cut(s)	717, 1072
SspMI	CTAG	7 cut(s)	101, 405, 527, 662, 1119, 1329, 1503
SstI	GAGCTC	1 cut(s)	1770
StuI	AGGCCT	2 cut(s)	423, 1256
StyD4I	CCNGG	4 cut(s)	772, 1408, 1489, 1579
StyI	CCWWGG	4 cut(s)	502, 526, 1386, 1502
TaaI	ACNGT	4 cut(s)	83, 222, 354, 569
TaiI	ACGT	1 cut(s)	378
TaqI	TCGA	9 cut(s)	48, 76, 130, 291, 463, 547, 948, 1066, 1182
TatI	WGTACW	1 cut(s)	1368
TauI	GCSGC	1 cut(s)	1365
TfiI	GAWTC	6 cut(s)	169, 1049, 1379, 1628, 1694, 1732
Tru1I	TTAA	5 cut(s)	177, 243, 270, 780, 1674
Tru9I	TTAA	5 cut(s)	177, 243, 270, 780, 1674
TscAI	CASTG	6 cut(s)	12, 76, 574, 1000, 1108, 1462
TseFI	GTSAC	6 cut(s)	476, 521, 670, 727, 763, 1453
TseI	GCWGC	4 cut(s)	536, 707, 1497, 1652
Tsp45I	GTSAC	6 cut(s)	476, 521, 670, 727, 763, 1453
TspDTI	ATGAA	7 cut(s)	156, 224, 425, 778, 1533, 1673, 1797
TspGWI	ACGGA	1 cut(s)	338
TspRI	CASTG	6 cut(s)	12, 76, 574, 1000, 1108, 1462
Van91I	CCANNNNNTGG	1 cut(s)	1553
Vha464I	CTTAAG	1 cut(s)	242
VpaK11BI	GGWCC	2 cut(s)	964, 1318
VspI	ATTAAT	1 cut(s)	1674
XapI	RAATTY	4 cut(s)	1228, 1402, 1435, 1540
XceI	RCATGY	1 cut(s)	655
XmaJI	CCTAGG	2 cut(s)	526, 1502
XmiI	GTMKAC	1 cut(s)	633
XmnI	GAANNNNTTC	1 cut(s)	1128
XspI	CTAG	7 cut(s)	101, 405, 527, 662, 1119, 1329, 1503
ZrmI	AGTACT	1 cut(s)	1370
Zsp2I	ATGCAT	1 cut(s)	803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.